mRNA_M-pyrifera_M_contig88635.20579.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig88635.20579.1
Unique NamemRNA_M-pyrifera_M_contig88635.20579.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig88635.20579.1 vs. uniprot
Match: A0A5A8DTK3_CAFRO (Cytochrome b-c1 complex subunit Rieske, mitochondrial n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DTK3_CAFRO)

HSP 1 Score: 246 bits (629), Expect = 2.610e-80
Identity = 112/164 (68.29%), Postives = 140/164 (85.37%), Query Frame = 1 
Query:    1 RASTYFLLGGAKALYASAARIAIIRAVTTMTASADVLALASLEVDVSSIPAGQTVTVKWRGKPVFIRHRSPKQVEEVSGGTPAGLRDPADDAERTQKPDWLIVMGICTHLGCVPMADMGDYGGWFCPCHASHYDGSGRIRQGPAPLNLEVPPYKFIDDNTVLLG 492
            RA TYF+LGGAK +YAS  R AI+ A+ TM+ASADVLALAS+EVD+ +IP  +TVTVKWRGKPVFIR+R+ +Q+ E +  TPAGLRDP  DAERT+ P WL+++GICTHLGCVPM++ GDY GW+CPCH SHYDGSGRIR+GPAPLNLE+PPYKF+ ++ ++LG
Sbjct:   80 RAFTYFVLGGAKFVYASTVRFAIVNALATMSASADVLALASVEVDLGAIPLSETVTVKWRGKPVFIRNRTEEQIAECAD-TPAGLRDPQTDAERTENPKWLVILGICTHLGCVPMSNAGDYNGWYCPCHGSHYDGSGRIRRGPAPLNLEIPPYKFVGEDKLILG 242
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BLAST of mRNA_M-pyrifera_M_contig88635.20579.1 vs. uniprot
Match: A0A812MJ19_9DINO (Cytochrome b-c1 complex subunit Rieske, mitochondrial n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A812MJ19_9DINO)

HSP 1 Score: 238 bits (607), Expect = 3.200e-77
Identity = 108/164 (65.85%), Postives = 137/164 (83.54%), Query Frame = 1 
Query:    1 RASTYFLLGGAKALYASAARIAIIRAVTTMTASADVLALASLEVDVSSIPAGQTVTVKWRGKPVFIRHRSPKQVEEVSGGTPAGLRDPADDAERTQKPDWLIVMGICTHLGCVPMADMGDYGGWFCPCHASHYDGSGRIRQGPAPLNLEVPPYKFIDDNTVLLG 492
            RA TYF+LGGA+ LYASA R+A++R + T++ASADVLALA+LEVD+ ++  G+ +TVKWRGKPVF++HR+  Q+ EV+     GLRDP  DAERTQ P WL+V+GICTHLGCVP+A+ G++ GWFCPCH SHYDGSGRIRQGPAPLNLEVPPYKF+  + ++LG
Sbjct:   62 RAFTYFVLGGARFLYASAVRVAVVRTLATLSASADVLALANLEVDLKAVQPGECITVKWRGKPVFVKHRTEDQIAEVAAPL-QGLRDPQTDAERTQDPKWLVVLGICTHLGCVPIANAGNFKGWFCPCHGSHYDGSGRIRQGPAPLNLEVPPYKFVGGDKLILG 224
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BLAST of mRNA_M-pyrifera_M_contig88635.20579.1 vs. uniprot
Match: W2M1B7_PHYPR (Cytochrome b-c1 complex subunit Rieske, mitochondrial n=10 Tax=Peronosporaceae TaxID=4777 RepID=W2M1B7_PHYPR)

HSP 1 Score: 237 bits (605), Expect = 4.800e-77
Identity = 107/164 (65.24%), Postives = 137/164 (83.54%), Query Frame = 1 
Query:    1 RASTYFLLGGAKALYASAARIAIIRAVTTMTASADVLALASLEVDVSSIPAGQTVTVKWRGKPVFIRHRSPKQVEEVSGGTPAGLRDPADDAERTQKPDWLIVMGICTHLGCVPMADMGDYGGWFCPCHASHYDGSGRIRQGPAPLNLEVPPYKFIDDNTVLLG 492
            RA TYF+LGGA+  YA+AAR+A+++ V +M+ASADVLALA+ E D+SSI  G TVTVKWRGKP+FI+HR+ K+++  +    + LRDP  DA R QKP+WL+V+G+CTHLGCVP +D G+YGGWFCPCH SHYD SGRIR+GPAPLNLE+PPYKFI+D+ +LLG
Sbjct:   52 RAFTYFMLGGARVAYATAARLAVVKFVGSMSASADVLALATAEFDLSSINEGSTVTVKWRGKPIFIKHRTAKEIDISNQVDVSNLRDPETDAVRVQKPEWLVVLGVCTHLGCVPTSDAGEYGGWFCPCHGSHYDLSGRIRKGPAPLNLEIPPYKFIEDDKILLG 215
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BLAST of mRNA_M-pyrifera_M_contig88635.20579.1 vs. uniprot
Match: M4B2Y1_HYAAE (Cytochrome b-c1 complex subunit Rieske, mitochondrial n=1 Tax=Hyaloperonospora arabidopsidis (strain Emoy2) TaxID=559515 RepID=M4B2Y1_HYAAE)

HSP 1 Score: 234 bits (598), Expect = 5.940e-76
Identity = 106/164 (64.63%), Postives = 136/164 (82.93%), Query Frame = 1 
Query:    1 RASTYFLLGGAKALYASAARIAIIRAVTTMTASADVLALASLEVDVSSIPAGQTVTVKWRGKPVFIRHRSPKQVEEVSGGTPAGLRDPADDAERTQKPDWLIVMGICTHLGCVPMADMGDYGGWFCPCHASHYDGSGRIRQGPAPLNLEVPPYKFIDDNTVLLG 492
            RA TYF+LGGA+  YA+AAR+A+++ V +M+ASADVLAL++ E D+S+I  G TVTVKWRGKP+FI+HR+ K+++       + LRDP  DA R QKP+WL+V+G+CTHLGCVP ++ G+YGGWFCPCH SHYD SGRIR+GPAPLNLE+PPYKFI+DN VLLG
Sbjct:   54 RAFTYFMLGGARVAYATAARLAVVKFVGSMSASADVLALSTAEFDLSNISEGSTVTVKWRGKPIFIKHRTAKEIDISEQVDVSHLRDPETDAVRVQKPEWLVVLGVCTHLGCVPTSNAGEYGGWFCPCHGSHYDLSGRIRKGPAPLNLEIPPYKFIEDNKVLLG 217
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BLAST of mRNA_M-pyrifera_M_contig88635.20579.1 vs. uniprot
Match: H3GII1_PHYRM (Cytochrome b-c1 complex subunit Rieske, mitochondrial n=13 Tax=Peronosporaceae TaxID=4777 RepID=H3GII1_PHYRM)

HSP 1 Score: 234 bits (596), Expect = 1.160e-75
Identity = 104/164 (63.41%), Postives = 136/164 (82.93%), Query Frame = 1 
Query:    1 RASTYFLLGGAKALYASAARIAIIRAVTTMTASADVLALASLEVDVSSIPAGQTVTVKWRGKPVFIRHRSPKQVEEVSGGTPAGLRDPADDAERTQKPDWLIVMGICTHLGCVPMADMGDYGGWFCPCHASHYDGSGRIRQGPAPLNLEVPPYKFIDDNTVLLG 492
            RA TYF+LGGA+  YA+AAR+A+++ V +M+ASADVLALA+ E D+S+I  G TVTVKWRGKP+FI+HR+ K+++  +      LRDP  DA R QKP+WL+V+G+CTHLGCVP +D G+YGGWFCPCH SHYD SGRIR+GPAPLNLE+PPY+F+++N +LLG
Sbjct:   53 RAFTYFMLGGARVAYATAARLAVVKFVGSMSASADVLALATAEFDLSNINEGSTVTVKWRGKPIFIKHRTAKEIDISNQVDVGNLRDPETDAVRVQKPEWLVVLGVCTHLGCVPTSDAGEYGGWFCPCHGSHYDLSGRIRKGPAPLNLEIPPYEFLEENKILLG 216
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BLAST of mRNA_M-pyrifera_M_contig88635.20579.1 vs. uniprot
Match: A0A2D4CB52_PYTIN (Cytochrome b-c1 complex subunit Rieske, mitochondrial n=2 Tax=Pythium TaxID=4797 RepID=A0A2D4CB52_PYTIN)

HSP 1 Score: 233 bits (594), Expect = 2.660e-75
Identity = 106/164 (64.63%), Postives = 135/164 (82.32%), Query Frame = 1 
Query:    1 RASTYFLLGGAKALYASAARIAIIRAVTTMTASADVLALASLEVDVSSIPAGQTVTVKWRGKPVFIRHRSPKQVEEVSGGTPAGLRDPADDAERTQKPDWLIVMGICTHLGCVPMADMGDYGGWFCPCHASHYDGSGRIRQGPAPLNLEVPPYKFIDDNTVLLG 492
            RA TYF+LGGA+  YA+AAR+A+I+ V +M+ASADVLALA+ E D+S+I  G TVTVKWRGKP+FI+HRS K++   +    + LRDP  DAER + P WL+V+G+CTHLGCVP ++ G+YGGWFCPCH SHYD SGRIR+GPAPLNLE+PPY+FI+DN +LLG
Sbjct:   57 RAFTYFMLGGARVAYATAARLAVIKFVGSMSASADVLALATAEFDLSNIDEGATVTVKWRGKPIFIKHRSEKEIALSNDVNLSNLRDPETDAERVKDPKWLVVLGVCTHLGCVPTSNAGEYGGWFCPCHGSHYDLSGRIRKGPAPLNLEIPPYQFIEDNKILLG 220
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BLAST of mRNA_M-pyrifera_M_contig88635.20579.1 vs. uniprot
Match: A0A5D6XVF7_9STRA (Cytochrome b-c1 complex subunit Rieske, mitochondrial n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XVF7_9STRA)

HSP 1 Score: 232 bits (591), Expect = 6.880e-75
Identity = 105/164 (64.02%), Postives = 134/164 (81.71%), Query Frame = 1 
Query:    1 RASTYFLLGGAKALYASAARIAIIRAVTTMTASADVLALASLEVDVSSIPAGQTVTVKWRGKPVFIRHRSPKQVEEVSGGTPAGLRDPADDAERTQKPDWLIVMGICTHLGCVPMADMGDYGGWFCPCHASHYDGSGRIRQGPAPLNLEVPPYKFIDDNTVLLG 492
            RA TYFLLGGA+  YA+A R+A+I+ V +M+ASADVLALA+ E D+S+I  G TVTVKWRGKP+FI+HR+ K++E  +    + LRDP  DA R Q P WL+V+G+CTHLGCVP ++ G+YGGWFCPCH SHYD SGRIR+GPAPLNLE+PPYKF+++N +LLG
Sbjct:   54 RAFTYFLLGGARVAYATAGRLAVIKFVGSMSASADVLALATAEFDLSNIDEGATVTVKWRGKPIFIKHRTDKEIELSNAVNVSNLRDPETDAVRVQDPKWLVVLGVCTHLGCVPTSNAGEYGGWFCPCHGSHYDLSGRIRKGPAPLNLEIPPYKFLENNKILLG 217
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BLAST of mRNA_M-pyrifera_M_contig88635.20579.1 vs. uniprot
Match: A0A7S3K5M4_9STRA (Cytochrome b-c1 complex subunit Rieske, mitochondrial n=1 Tax=Aureoumbra lagunensis TaxID=44058 RepID=A0A7S3K5M4_9STRA)

HSP 1 Score: 230 bits (586), Expect = 4.090e-74
Identity = 106/164 (64.63%), Postives = 131/164 (79.88%), Query Frame = 1 
Query:    1 RASTYFLLGGAKALYASAARIAIIRAVTTMTASADVLALASLEVDVSSIPAGQTVTVKWRGKPVFIRHRSPKQVEEVSGGTPAGLRDPADDAERTQKPDWLIVMGICTHLGCVPMADMGDYGGWFCPCHASHYDGSGRIRQGPAPLNLEVPPYKFIDDNTVLLG 492
            R  TY ++G  K +YASA RIA I+ + +M AS DVLALAS E DVS++ AG+T+TVKWRGKPVFIRHR+ ++V E  G +   LRDP  DA+R Q+ DWL+VMGICTHLGCVP+A+ G+Y GWFCPCH SHYD SGRIR+GPAPLNLEVPPYKF+D + +L+G
Sbjct:   55 RGFTYLVMGANKFIYASAVRIAAIKFIASMAASKDVLALASAEFDVSAVEAGKTITVKWRGKPVFIRHRTSEEVAEAEGVSLDALRDPQSDADRVQEKDWLVVMGICTHLGCVPIANAGEYNGWFCPCHGSHYDVSGRIRKGPAPLNLEVPPYKFLDSDKLLVG 218
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BLAST of mRNA_M-pyrifera_M_contig88635.20579.1 vs. uniprot
Match: F0W758_9STRA (Cytochrome b-c1 complex subunit Rieske, mitochondrial n=2 Tax=Albugo TaxID=65356 RepID=F0W758_9STRA)

HSP 1 Score: 229 bits (585), Expect = 6.200e-74
Identity = 103/164 (62.80%), Postives = 135/164 (82.32%), Query Frame = 1 
Query:    1 RASTYFLLGGAKALYASAARIAIIRAVTTMTASADVLALASLEVDVSSIPAGQTVTVKWRGKPVFIRHRSPKQVEEVSGGTPAGLRDPADDAERTQKPDWLIVMGICTHLGCVPMADMGDYGGWFCPCHASHYDGSGRIRQGPAPLNLEVPPYKFIDDNTVLLG 492
            RA TYF+LGGA+ +YA+AAR+A+I+ V +M+ASADVLALA+ E D+SSI  G TVTVKWRGKP+FI+HR+ +++E  +    + LRDP  D  R + P WL+V+G+CTHLGCVP+++ GDY GWFCPCH SHYD SGRIR+GPAPLNLE+PPY+F++DN +LLG
Sbjct:   57 RAFTYFVLGGARIMYATAARLAVIKFVGSMSASADVLALATAEFDLSSIDEGATVTVKWRGKPIFIKHRTDQEIESANDVPVSSLRDPETDDVRVKDPKWLVVLGVCTHLGCVPISNAGDYKGWFCPCHGSHYDLSGRIRKGPAPLNLEIPPYQFLEDNKILLG 220
[Close]
BLAST of mRNA_M-pyrifera_M_contig88635.20579.1 vs. uniprot
Match: K3WIU0_GLOUD (Cytochrome b-c1 complex subunit Rieske, mitochondrial n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WIU0_GLOUD)

HSP 1 Score: 229 bits (583), Expect = 1.130e-73
Identity = 106/164 (64.63%), Postives = 132/164 (80.49%), Query Frame = 1 
Query:    1 RASTYFLLGGAKALYASAARIAIIRAVTTMTASADVLALASLEVDVSSIPAGQTVTVKWRGKPVFIRHRSPKQVEEVSGGTPAGLRDPADDAERTQKPDWLIVMGICTHLGCVPMADMGDYGGWFCPCHASHYDGSGRIRQGPAPLNLEVPPYKFIDDNTVLLG 492
            RA TYFLLGGA+  YA+A R+A+I+ V +M+ASADVLALA+ E D+S+I  G TVTVKWRGKP+FI+HR+ K++   +    A L+DP  D  R Q P WLIV+G+CTHLGCVP ++ G+YGGWFCPCH SHYD SGRIR+GPAPLNLE+PPYKFI++N VLLG
Sbjct:   54 RAFTYFLLGGARVAYATAGRLAVIKFVGSMSASADVLALATAEFDLSNIDEGATVTVKWRGKPIFIKHRTDKEITISNEVNVANLKDPETDDVRVQNPKWLIVLGVCTHLGCVPTSNAGEYGGWFCPCHGSHYDLSGRIRKGPAPLNLEIPPYKFIEENKVLLG 217
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig88635.20579.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8DTK3_CAFRO2.610e-8068.29Cytochrome b-c1 complex subunit Rieske, mitochondr... [more]
A0A812MJ19_9DINO3.200e-7765.85Cytochrome b-c1 complex subunit Rieske, mitochondr... [more]
W2M1B7_PHYPR4.800e-7765.24Cytochrome b-c1 complex subunit Rieske, mitochondr... [more]
M4B2Y1_HYAAE5.940e-7664.63Cytochrome b-c1 complex subunit Rieske, mitochondr... [more]
H3GII1_PHYRM1.160e-7563.41Cytochrome b-c1 complex subunit Rieske, mitochondr... [more]
A0A2D4CB52_PYTIN2.660e-7564.63Cytochrome b-c1 complex subunit Rieske, mitochondr... [more]
A0A5D6XVF7_9STRA6.880e-7564.02Cytochrome b-c1 complex subunit Rieske, mitochondr... [more]
A0A7S3K5M4_9STRA4.090e-7464.63Cytochrome b-c1 complex subunit Rieske, mitochondr... [more]
F0W758_9STRA6.200e-7462.80Cytochrome b-c1 complex subunit Rieske, mitochondr... [more]
K3WIU0_GLOUD1.130e-7364.63Cytochrome b-c1 complex subunit Rieske, mitochondr... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig88635contigM-pyrifera_M_contig88635:2..496 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score240.0
Seed ortholog evalue8e-61
Seed eggNOG ortholog4787.PITG_05840T0
KEGG koko:K00411
KEGG Pathwayko00190,ko01100,ko02020,ko04260,ko04714,ko04932,ko05010,ko05012,ko05016,map00190,map01100,map02020,map04260,map04714,map04932,map05010,map05012,map05016
KEGG ModuleM00151,M00152
Hectar predicted targeting categorysignal peptide
GOsGO:0003674,GO:0003824,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005740,GO:0005743,GO:0005746,GO:0005750,GO:0006091,GO:0006950,GO:0008121,GO:0008150,GO:0008152,GO:0009055,GO:0009060,GO:0009266,GO:0009408,GO:0009628,GO:0009987,GO:0015980,GO:0016020,GO:0016491,GO:0016679,GO:0016681,GO:0019866,GO:0022900,GO:0031090,GO:0031966,GO:0031967,GO:0031975,GO:0032991,GO:0043167,GO:0043169,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044422,GO:0044424,GO:0044425,GO:0044429,GO:0044444,GO:0044446,GO:0044455,GO:0044464,GO:0045275,GO:0045333,GO:0046872,GO:0050896,GO:0055114,GO:0070069,GO:0070469,GO:0098796,GO:0098798,GO:0098800,GO:0098803,GO:1902494,GO:1990204
EggNOG free text desc.Component of the ubiquinol-cytochrome c reductase complex (complex III or cytochrome b-c1 complex), which is a respiratory chain that generates an electrochemical potential coupled to ATP synthesis
EggNOG OGs3QE83@4776,COG0723@1,KOG1671@2759
EC1.10.2.2
COG Functional cat.C
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Exons1
Model size495
Cds size495
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816796.6518362-CDS-M-pyrifera_M_contig88635:1..4961622816796.6518362-CDS-M-pyrifera_M_contig88635:1..496Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig88635 2..496 +
1692278183.9008212-CDS-M-pyrifera_M_contig88635:1..4961692278183.9008212-CDS-M-pyrifera_M_contig88635:1..496Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig88635 2..496 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig88635.20579.1prot_M-pyrifera_M_contig88635.20579.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig88635 2..496 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig88635.20579.1

>prot_M-pyrifera_M_contig88635.20579.1 ID=prot_M-pyrifera_M_contig88635.20579.1|Name=mRNA_M-pyrifera_M_contig88635.20579.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=165bp
RASTYFLLGGAKALYASAARIAIIRAVTTMTASADVLALASLEVDVSSIP
AGQTVTVKWRGKPVFIRHRSPKQVEEVSGGTPAGLRDPADDAERTQKPDW
LIVMGICTHLGCVPMADMGDYGGWFCPCHASHYDGSGRIRQGPAPLNLEV
PPYKFIDDNTVLLG*
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mRNA from alignment at M-pyrifera_M_contig88635:2..496+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig88635.20579.1 ID=mRNA_M-pyrifera_M_contig88635.20579.1|Name=mRNA_M-pyrifera_M_contig88635.20579.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=495bp|location=Sequence derived from alignment at M-pyrifera_M_contig88635:2..496+ (Macrocystis pyrifera P11B4 male)
CGCGCTTCTACGTATTTCCTGTTGGGCGGGGCCAAGGCCCTGTACGCTAG
CGCGGCTCGCATTGCGATCATCCGCGCGGTGACGACGATGACGGCATCTG
CGGACGTGTTGGCCTTGGCTTCGTTGGAGGTGGACGTGTCCTCGATTCCT
GCGGGGCAGACTGTGACGGTCAAGTGGCGAGGCAAGCCCGTGTTCATTCG
CCACCGCAGCCCCAAACAGGTGGAGGAGGTGTCGGGAGGAACACCTGCTG
GTTTGCGAGACCCGGCTGATGACGCGGAACGCACCCAGAAGCCAGACTGG
CTCATCGTGATGGGCATCTGCACCCATTTGGGATGTGTCCCCATGGCGGA
CATGGGAGACTACGGGGGATGGTTCTGCCCCTGCCACGCCTCGCACTACG
ACGGGTCCGGCAGAATCAGACAAGGACCAGCTCCCCTCAACCTGGAAGTC
CCACCCTACAAGTTCATCGACGACAACACTGTGCTTCTCGGGTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig88635:2..496+

>mRNA_M-pyrifera_M_contig88635.20579.1 ID=mRNA_M-pyrifera_M_contig88635.20579.1|Name=mRNA_M-pyrifera_M_contig88635.20579.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=990bp|location=Sequence derived from alignment at M-pyrifera_M_contig88635:2..496+ (Macrocystis pyrifera P11B4 male)
CGCGCTTCTACGTATTTCCTGTTGGGCGGGGCCAAGGCCCTGTACGCTAG
CGCGGCTCGCATTGCGATCATCCGCGCGGTGACGACGATGACGGCATCTG
CGGACGTGTTGGCCTTGGCTTCGTTGGAGGTGGACGTGTCCTCGATTCCT
GCGGGGCAGACTGTGACGGTCAAGTGGCGAGGCAAGCCCGTGTTCATTCG
CCACCGCAGCCCCAAACAGGTGGAGGAGGTGTCGGGAGGAACACCTGCTG
GTTTGCGAGACCCGGCTGATGACGCGGAACGCACCCAGAAGCCAGACTGG
CTCATCGTGATGGGCATCTGCACCCATTTGGGATGTGTCCCCATGGCGGA
CATGGGAGACTACGGGGGATGGTTCTGCCCCTGCCACGCCTCGCACTACG
ACGGGTCCGGCAGAATCAGACAAGGACCAGCTCCCCTCAACCTGGAAGTC
CCACCCTACAAGTTCATCGACGACAACACTGTGCTTCTCGGGTGACGCGC
TTCTACGTATTTCCTGTTGGGCGGGGCCAAGGCCCTGTACGCTAGCGCGG
CTCGCATTGCGATCATCCGCGCGGTGACGACGATGACGGCATCTGCGGAC
GTGTTGGCCTTGGCTTCGTTGGAGGTGGACGTGTCCTCGATTCCTGCGGG
GCAGACTGTGACGGTCAAGTGGCGAGGCAAGCCCGTGTTCATTCGCCACC
GCAGCCCCAAACAGGTGGAGGAGGTGTCGGGAGGAACACCTGCTGGTTTG
CGAGACCCGGCTGATGACGCGGAACGCACCCAGAAGCCAGACTGGCTCAT
CGTGATGGGCATCTGCACCCATTTGGGATGTGTCCCCATGGCGGACATGG
GAGACTACGGGGGATGGTTCTGCCCCTGCCACGCCTCGCACTACGACGGG
TCCGGCAGAATCAGACAAGGACCAGCTCCCCTCAACCTGGAAGTCCCACC
CTACAAGTTCATCGACGACAACACTGTGCTTCTCGGGTGA
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