mRNA_M-pyrifera_M_contig86703.20167.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig86703.20167.1
Unique NamemRNA_M-pyrifera_M_contig86703.20167.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig86703.20167.1 vs. uniprot
Match: A0A0G4F917_VITBC (Uncharacterized protein n=2 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4F917_VITBC)

HSP 1 Score: 71.2 bits (173), Expect = 2.720e-11
Identity = 51/178 (28.65%), Postives = 80/178 (44.94%), Query Frame = 1
Query:    7 KEVKGNILKEARILE---------ALKDHPNFPEFLGTCYHSGFTHIVMHFERSGNALEYLSSKHRQLRENHMLFLARGVVQALQALHNEGIAHRDVTADNVLVHIDKLNKVVGILADLGNAIPWEWDWSGSHQAHALYEPPELISSLEHKMFPEGLPPVIDMRPADVFQFGVTLWEM 513
            KE +   ++EAR+L          A   HPN   F+G C  + +  IV  +  +G+  + L  +H++L++  +L + +G+  A+  LH  GI H D+ + N+L+      K    L D G A+      S  H+               H M PE L        ADV+ FG+ LWEM
Sbjct:  444 KEFRRTGIEEARVLREFATEVSTLAQLRHPNIALFMGICVEAKYQCIVSEYVGNGSLFDLLHRRHQKLQQLSLLQICQGICSAMAYLHGLGIIHCDLKSSNILLDYSLNPK----LCDFGLALNLLRQRSSKHRGRV---------GTPHWMAPEVLRGAECTPAADVYSFGILLWEM 608          
BLAST of mRNA_M-pyrifera_M_contig86703.20167.1 vs. uniprot
Match: A0A5J4YEC3_9CHLO (Cytochrome P450 n=1 Tax=Trebouxia sp. A1-2 TaxID=2608996 RepID=A0A5J4YEC3_9CHLO)

HSP 1 Score: 66.2 bits (160), Expect = 1.510e-9
Identity = 51/181 (28.18%), Postives = 82/181 (45.30%), Query Frame = 1
Query:   10 EVKGNILKEARILEALKDHPNFPEFLGTCYHSGFTHIVMHFERSGN---ALEYLSSKHRQLR-ENHMLFLARGVVQALQALHNEGIAHRDVTADNVLVHIDK---------LNKVVGILADLGNAIPWEWDWSGSHQAHALYEPPELISSLEHKMFPEGLPPVIDMRPADVFQFGVTLWEM 513
            E   NI +E ++ + L    N  +F G+C   G   +V+ +   G+   AL+ L SK  QLR  N    +A  V++ L  LH  G+ HRD+ + NVL+  D          L  ++G  ++ G+++          QA   Y  PE++    HK              AD+F +G+ LWE+
Sbjct:  630 EAADNIDRELQMWKVLSFDKNLVQFYGSCEQDGKVLLVLEYMEGGDLRHALKGLGSKAAQLRWHNKGAHIALDVIKGLHFLHTHGVLHRDIKSGNVLLSGDYSQAKICDVGLAHIMGSTSNTGHSV----------QATFAYAAPEML--FNHKC----------NEKADIFSYGIVLWEI 788          
BLAST of mRNA_M-pyrifera_M_contig86703.20167.1 vs. uniprot
Match: A0A015LU85_RHIIW (Bck1p n=4 Tax=Rhizophagus irregularis TaxID=588596 RepID=A0A015LU85_RHIIW)

HSP 1 Score: 65.1 bits (157), Expect = 3.940e-9
Identity = 49/165 (29.70%), Postives = 76/165 (46.06%), Query Frame = 1
Query:   25 ILKEARILEALKDHPNFPEFLGTCYHSGFTHIVMHFERSGNALEYLSSKHRQLRENHMLFLARGVVQALQALHNEGIAHRDVTADNVLVHIDKLNKVVGILADLGNAIPWEWDWSGSHQAHAL--YEPPELISSLEHKMFPEGLPPVIDMRPADVFQFGVTLWEM 513
            I+ E ++   +  H N   F G         +VM +   G+   YL     +L  N    LA  +  A++ LHNEGI H D+ A NVLVH DK+      LAD G +   +  +S S     +  Y  P  ++++++K     L P      +DV+  GV LW++
Sbjct:   72 IINELKMQREVDYHANIIRFYGISKFKNQYLLVMEYADCGSLQSYLRKNFNKLEWNDKYQLAFQLANAVECLHNEGIIHCDLHAQNVLVHQDKIK-----LADFGLSRKIDDIYSNSTDVLGVVPYVDPRYLNNIKNKNQLYKLNP-----KSDVYSVGVLLWQI 226          
BLAST of mRNA_M-pyrifera_M_contig86703.20167.1 vs. uniprot
Match: J9I5I7_9SPIT (Serine/threonine protein kinase n=1 Tax=Oxytricha trifallax TaxID=1172189 RepID=J9I5I7_9SPIT)

HSP 1 Score: 63.9 bits (154), Expect = 9.300e-9
Identity = 47/172 (27.33%), Postives = 81/172 (47.09%), Query Frame = 1
Query:    1 MSKEVKGNILKEARILEALKDHPNFPEFLGTCYHSG-FTHIVMHFERSGNALEYLSSKHRQLRENHMLFLARGVVQALQALHNEGIAHRDVTADNVLVHIDKLNKVVGILADLGNAIPWEWDWSGSHQAHALYEPPELISSLEHKMFPEGLPPVIDMRPADVFQFGVTLWEM 513
            ++K+   + +KE  ++  L+ HPN   ++G  + +  F +++  F   G+  E L  K   L ++  + +A+ +  ALQ +H + I H D+ + N+L++ D   K+     D G A   E     +H                H M PE L     + PADV+ +GV LWEM
Sbjct:  503 LTKKAVKDFIKEIEVVNQLR-HPNIVLYMGVTFDTNNFYYMITEFVNKGSLFELLHQKKIPLDDDKTMKIAKQMAMALQYIHRKKILHCDLKSQNILLNDDWTVKI----CDFGLARYREKFQKDNHGKIGT----------PHWMAPEILRGEKYLEPADVYSYGVILWEM 659          
BLAST of mRNA_M-pyrifera_M_contig86703.20167.1 vs. uniprot
Match: A0A1X7S5T4_ZYMTR (Protein kinase domain-containing protein n=4 Tax=Zymoseptoria TaxID=1047167 RepID=A0A1X7S5T4_ZYMTR)

HSP 1 Score: 63.5 bits (153), Expect = 1.380e-8
Identity = 42/152 (27.63%), Postives = 71/152 (46.71%), Query Frame = 1
Query:   61 DHPNFPEFLGTCYHSGFTHIVMHFERSGNALEYLSSKHRQLRENHMLFLARGVVQALQALHNEGIAHRDVTADNVLVHIDKLNKVVGILADLGNAIPWEWDWSGSHQAHALYEPPELISSLEHKM-FPEGLPPVIDMRPADVFQFGVTLWEM 513
            DH N  ++LG C    ++  +     SG ++     KH +  E  +  L R  +  L  LH+EGI HRD+ ADN+L+ +D   K+        +A P+  D + S Q    +  PE+I +    +  P G    +D+R +  +   V +W +
Sbjct: 1506 DHVNIVQYLG-CEKKEYSISIFLEYISGGSVGSCLRKHGKFEEPVVSSLTRQTLGGLAYLHSEGILHRDLKADNILLDLDGTCKISDFGISKRSANPYNNDITNSMQGSVFWMAPEVIRAQSQALSVPSGANDGVDVRMSQGYSAKVDIWSL 1656          
BLAST of mRNA_M-pyrifera_M_contig86703.20167.1 vs. uniprot
Match: UPI001F5C97FE (serine/threonine-protein kinase STY46-like isoform X2 n=2 Tax=Lolium rigidum TaxID=89674 RepID=UPI001F5C97FE)

HSP 1 Score: 63.2 bits (152), Expect = 1.530e-8
Identity = 47/174 (27.01%), Postives = 81/174 (46.55%), Query Frame = 1
Query:    1 MSKEVKGNILKEARILEALKDHPNFPEFLGTCYHSGFTHIVMHFERSGNALEYLSSKHRQLRENHMLFLARGVVQALQALHNEGIAHRDVTADNVLVHIDKLNKVVGILADLGNAIPWEWDWSGSHQAHA---LYEPPELISSLEHKMFPEGLPPVIDMRPADVFQFGVTLWEM 513
            +S ++  +  +E  I+  ++ H N  +F+G C      +I+  F   G+  +YL  K+   +   +L +A  + + +  LH   I HRD+   N+L+  +K+ KV    AD G  +    D SG   A      +  PE+I   EHK +            AD+F FG+ +WE+
Sbjct:  203 ISADMYQDFAQEVYIMRKVR-HKNVVQFIGACTRQPNLYIITDFMSGGSVYDYLHKKNSSFKLPEILRVATDIAKGMNYLHQNNIIHRDLKTANLLMDENKVVKV----ADFG--VARVKDQSGVMTAETGTYRWMAPEVI---EHKPYD---------HKADIFSFGIVVWEL 357          
BLAST of mRNA_M-pyrifera_M_contig86703.20167.1 vs. uniprot
Match: UPI001FB10E1A (serine/threonine/tyrosine-protein kinase HT1-like n=1 Tax=Impatiens glandulifera TaxID=253017 RepID=UPI001FB10E1A)

HSP 1 Score: 62.8 bits (151), Expect = 1.770e-8
Identity = 43/156 (27.56%), Postives = 71/156 (45.51%), Query Frame = 1
Query:   64 HPNFPEFLGTCYHSGFTHIVMHFERSGNALEYLSSKHR-QLRENHMLFLARGVVQALQALHNEGIAHRDVTADNVLVHIDKLNKVVGILADLGNAI-----PWEWDWSGSHQAHALYEPPELISSLEHKMFPEGLPPVIDMRPADVFQFGVTLWEM 513
            HPN  +F+  C       I++ +   GN   YL+ K    L    +L LA  + + +  LH+EG+ HRD+ ++N+L++ D   KV    AD G +          +W G+   +  +  PE+I    +             R  DV+ FG+ LWE+
Sbjct:  132 HPNIVQFIAACRKPPVNLIIIEYMSQGNLRMYLNKKEPYSLSTETILRLALDISRGMSYLHSEGVIHRDLKSNNLLLNDDMRVKV----ADFGTSCLETQCRKRREWKGNKGTYR-WMAPEMIKEKAYN------------RKVDVYSFGIVLWEL 270          
BLAST of mRNA_M-pyrifera_M_contig86703.20167.1 vs. uniprot
Match: UPI00092E8D38 (serine/threonine-protein kinase STY46-like isoform X5 n=1 Tax=Lupinus angustifolius TaxID=3871 RepID=UPI00092E8D38)

HSP 1 Score: 62.8 bits (151), Expect = 2.110e-8
Identity = 49/175 (28.00%), Postives = 82/175 (46.86%), Query Frame = 1
Query:    1 MSKEVKGNILKEARILEALKDHPNFPEFLGTCYHSGFTHIVMHFERSGNALEYLSSKHRQLRENHMLFLARGVVQALQALHNEGIAHRDVTADNVLVHIDKLNKVVGILADLGNAIPWEWDWSGSHQAHALYEPPELISSLEHKMFPEGLPPVIDMRP----ADVFQFGVTLWEM 513
            +++ ++    +E  IL  +K H N  E +G C      +IV  +   G+  ++L  +   L  + +L +A  V + L  LHN GI HRD+ + N+L+      K V  +AD G A               L +P  ++++ E   +    P VI+  P    AD+F FG+ LWE+
Sbjct:  284 LNENIQREFSREVYILRKIK-HKNVIELIGACTIPSSLYIVTEYMSGGSMYDFLHIQKAVLTLSSLLKVAIDVSEGLHCLHNHGIIHRDLKSANLLMD----GKGVVKIADFGVA--------------RLLDPTGIMTA-ETGTYRWMAPEVIEHNPYSHKADIFSFGIILWEL 438          
BLAST of mRNA_M-pyrifera_M_contig86703.20167.1 vs. uniprot
Match: A0A453N1F2_AEGTS (Protein kinase domain-containing protein n=1 Tax=Aegilops tauschii subsp. strangulata TaxID=200361 RepID=A0A453N1F2_AEGTS)

HSP 1 Score: 62.4 bits (150), Expect = 2.270e-8
Identity = 49/174 (28.16%), Postives = 80/174 (45.98%), Query Frame = 1
Query:    1 MSKEVKGNILKEARILEALKDHPNFPEFLGTCYHSGFTHIVMHFERSGNALEYLSSKHRQLRENHMLFLARGVVQALQALHNEGIAHRDVTADNVLVHIDKLNKVVGILADLGNAIPWEWDWSGSHQAHA---LYEPPELISSLEHKMFPEGLPPVIDMRPADVFQFGVTLWEM 513
            +S ++  +  +E  I+  ++ H N  +F+G C      +I+  F   G+  +YL  K    +   +L +A  + + +  LH   I HRD+   N+L+  +K+ KV    AD G  +    D SG   A      +  PE+I   EHK +            ADVF FG+ LWE+
Sbjct:   81 ISADMYRDFAQEVYIMRKVR-HRNVVQFIGACTRQPNLYIITDFMSGGSVYDYLHKKGSSFKLPEILRVATDISKGMSYLHQNNIIHRDLKTANLLMDENKVVKV----ADFG--VARVKDTSGVMTAETGTYRWMAPEVI---EHKPYD---------HKADVFSFGIVLWEL 235          
BLAST of mRNA_M-pyrifera_M_contig86703.20167.1 vs. uniprot
Match: A0A453N1Z2_AEGTS (Protein kinase domain-containing protein n=2 Tax=Triticinae TaxID=1648030 RepID=A0A453N1Z2_AEGTS)

HSP 1 Score: 61.6 bits (148), Expect = 2.340e-8
Identity = 46/153 (30.07%), Postives = 69/153 (45.10%), Query Frame = 1
Query:   64 HPNFPEFLGTCYHSGFTHIVMHFERSGNALEYLSSKHRQLRENHMLFLARGVVQALQALHNEGIAHRDVTADNVLVHIDKLNKVVGILADLGNAIPWEWDWSGSHQAHA---LYEPPELISSLEHKMFPEGLPPVIDMRPADVFQFGVTLWEM 513
            H N  +F+G C      +I+  F   G+  +YL  K    +   +L +A  + + +  LH   I HRD+   N+L+  +K+ KV    AD G  +    D SG   A      +  PE+I   EHK +            ADVF FG+ LWE+
Sbjct:   12 HRNVVQFIGACTRQPNLYIITDFMSGGSVYDYLHKKGSSFKLPEILRVATDISKGMSYLHQNNIIHRDLKTANLLMDENKVVKV----ADFG--VARVKDTSGVMTAETGTYRWMAPEVI---EHKPYD---------HKADVFSFGIVLWEL 146          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig86703.20167.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A0G4F917_VITBC2.720e-1128.65Uncharacterized protein n=2 Tax=Vitrella brassicaf... [more]
A0A5J4YEC3_9CHLO1.510e-928.18Cytochrome P450 n=1 Tax=Trebouxia sp. A1-2 TaxID=2... [more]
A0A015LU85_RHIIW3.940e-929.70Bck1p n=4 Tax=Rhizophagus irregularis TaxID=588596... [more]
J9I5I7_9SPIT9.300e-927.33Serine/threonine protein kinase n=1 Tax=Oxytricha ... [more]
A0A1X7S5T4_ZYMTR1.380e-827.63Protein kinase domain-containing protein n=4 Tax=Z... [more]
UPI001F5C97FE1.530e-827.01serine/threonine-protein kinase STY46-like isoform... [more]
UPI001FB10E1A1.770e-827.56serine/threonine/tyrosine-protein kinase HT1-like ... [more]
UPI00092E8D382.110e-828.00serine/threonine-protein kinase STY46-like isoform... [more]
A0A453N1F2_AEGTS2.270e-828.16Protein kinase domain-containing protein n=1 Tax=A... [more]
A0A453N1Z2_AEGTS2.340e-830.07Protein kinase domain-containing protein n=2 Tax=T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig86703contigM-pyrifera_M_contig86703:8..520 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeArthropoda
Seed ortholog score78.6
Seed ortholog evalue3.2e-12
Seed eggNOG ortholog103372.F4WNV8
KEGG koko:K08287,ko:K08841,ko:K08842
Hectar predicted targeting categoryother localisation
GOsGO:0001700,GO:0003674,GO:0003824,GO:0004672,GO:0004674,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0006464,GO:0006468,GO:0006793,GO:0006796,GO:0006807,GO:0006996,GO:0007010,GO:0007015,GO:0007275,GO:0007399,GO:0008150,GO:0008152,GO:0009790,GO:0009792,GO:0009966,GO:0009968,GO:0009987,GO:0010453,GO:0010454,GO:0010646,GO:0010648,GO:0010721,GO:0016043,GO:0016301,GO:0016310,GO:0016740,GO:0016772,GO:0016773,GO:0019538,GO:0022008,GO:0022603,GO:0023051,GO:0023057,GO:0030029,GO:0030036,GO:0030154,GO:0032501,GO:0032502,GO:0036211,GO:0043170,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044424,GO:0044464,GO:0045177,GO:0045501,GO:0045595,GO:0045596,GO:0045664,GO:0045665,GO:0045676,GO:0045677,GO:0045873,GO:0046532,GO:0046533,GO:0048519,GO:0048523,GO:0048583,GO:0048585,GO:0048699,GO:0048731,GO:0048856,GO:0048869,GO:0050767,GO:0050768,GO:0050789,GO:0050793,GO:0050794,GO:0051093,GO:0051239,GO:0051241,GO:0051960,GO:0051961,GO:0060284,GO:0065007,GO:0071704,GO:0071840,GO:0097435,GO:0140096,GO:1901564,GO:2000026,GO:2000027
EggNOG free text desc.Protein tyrosine kinase
EggNOG OGs2QTCP@2759,38BED@33154,3BCNJ@33208,3CX11@33213,3SG4H@50557,41W2S@6656,46E9U@7399,COG0515@1
EC2.7.12.1
COG Functional cat.T
Best tax levelHymenoptera
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko01001
Exons1
Model size513
Cds size513
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816776.2917175-CDS-M-pyrifera_M_contig86703:7..5201622816776.2917175-CDS-M-pyrifera_M_contig86703:7..520Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig86703 8..520 +
1692278171.7215781-CDS-M-pyrifera_M_contig86703:7..5201692278171.7215781-CDS-M-pyrifera_M_contig86703:7..520Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig86703 8..520 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig86703.20167.1prot_M-pyrifera_M_contig86703.20167.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig86703 8..520 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig86703.20167.1

>prot_M-pyrifera_M_contig86703.20167.1 ID=prot_M-pyrifera_M_contig86703.20167.1|Name=mRNA_M-pyrifera_M_contig86703.20167.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=171bp
MSKEVKGNILKEARILEALKDHPNFPEFLGTCYHSGFTHIVMHFERSGNA
LEYLSSKHRQLRENHMLFLARGVVQALQALHNEGIAHRDVTADNVLVHID
KLNKVVGILADLGNAIPWEWDWSGSHQAHALYEPPELISSLEHKMFPEGL
PPVIDMRPADVFQFGVTLWEM
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mRNA from alignment at M-pyrifera_M_contig86703:8..520+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig86703.20167.1 ID=mRNA_M-pyrifera_M_contig86703.20167.1|Name=mRNA_M-pyrifera_M_contig86703.20167.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=513bp|location=Sequence derived from alignment at M-pyrifera_M_contig86703:8..520+ (Macrocystis pyrifera P11B4 male)
ATGTCTAAAGAAGTAAAAGGCAACATTCTGAAGGAGGCCCGAATCCTGGA GGCGCTCAAGGACCACCCGAATTTCCCCGAGTTTCTGGGCACTTGCTATC ACAGTGGGTTCACTCACATTGTGATGCACTTCGAGAGGAGCGGCAATGCG TTGGAATACCTTTCTTCGAAGCACCGACAGCTGAGGGAGAACCACATGCT CTTCCTCGCACGGGGGGTGGTGCAGGCTTTGCAGGCGTTGCACAACGAAG GCATAGCTCACAGAGACGTGACCGCTGACAACGTGCTGGTGCACATCGAC AAGCTCAACAAGGTAGTCGGCATTCTGGCAGACCTCGGCAACGCCATCCC CTGGGAGTGGGACTGGTCTGGCAGTCACCAAGCCCACGCACTCTACGAGC CACCCGAGCTCATCTCCAGCCTCGAGCACAAGATGTTCCCAGAGGGCCTC CCGCCAGTGATTGACATGCGACCTGCAGACGTGTTCCAGTTCGGCGTTAC GTTGTGGGAGATG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig86703:8..520+

>mRNA_M-pyrifera_M_contig86703.20167.1 ID=mRNA_M-pyrifera_M_contig86703.20167.1|Name=mRNA_M-pyrifera_M_contig86703.20167.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1026bp|location=Sequence derived from alignment at M-pyrifera_M_contig86703:8..520+ (Macrocystis pyrifera P11B4 male)
ATGTCTAAAGAAGTAAAAGGCAACATTCTGAAGGAGGCCCGAATCCTGGA
GGCGCTCAAGGACCACCCGAATTTCCCCGAGTTTCTGGGCACTTGCTATC
ACAGTGGGTTCACTCACATTGTGATGCACTTCGAGAGGAGCGGCAATGCG
TTGGAATACCTTTCTTCGAAGCACCGACAGCTGAGGGAGAACCACATGCT
CTTCCTCGCACGGGGGGTGGTGCAGGCTTTGCAGGCGTTGCACAACGAAG
GCATAGCTCACAGAGACGTGACCGCTGACAACGTGCTGGTGCACATCGAC
AAGCTCAACAAGGTAGTCGGCATTCTGGCAGACCTCGGCAACGCCATCCC
CTGGGAGTGGGACTGGTCTGGCAGTCACCAAGCCCACGCACTCTACGAGC
CACCCGAGCTCATCTCCAGCCTCGAGCACAAGATGTTCCCAGAGGGCCTC
CCGCCAGTGATTGACATGCGACCTGCAGACGTGTTCCAGTTCGGCGTTAC
GTTGTGGGAGATGATGTCTAAAGAAGTAAAAGGCAACATTCTGAAGGAGG
CCCGAATCCTGGAGGCGCTCAAGGACCACCCGAATTTCCCCGAGTTTCTG
GGCACTTGCTATCACAGTGGGTTCACTCACATTGTGATGCACTTCGAGAG
GAGCGGCAATGCGTTGGAATACCTTTCTTCGAAGCACCGACAGCTGAGGG
AGAACCACATGCTCTTCCTCGCACGGGGGGTGGTGCAGGCTTTGCAGGCG
TTGCACAACGAAGGCATAGCTCACAGAGACGTGACCGCTGACAACGTGCT
GGTGCACATCGACAAGCTCAACAAGGTAGTCGGCATTCTGGCAGACCTCG
GCAACGCCATCCCCTGGGAGTGGGACTGGTCTGGCAGTCACCAAGCCCAC
GCACTCTACGAGCCACCCGAGCTCATCTCCAGCCTCGAGCACAAGATGTT
CCCAGAGGGCCTCCCGCCAGTGATTGACATGCGACCTGCAGACGTGTTCC
AGTTCGGCGTTACGTTGTGGGAGATG
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