mRNA_M-pyrifera_M_contig8554.19938.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig8554.19938.1
Unique NamemRNA_M-pyrifera_M_contig8554.19938.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig8554.19938.1 vs. uniprot
Match: D8LTU1_ECTSI (DAO domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LTU1_ECTSI)

HSP 1 Score: 177 bits (448), Expect = 5.300e-52
Identity = 89/108 (82.41%), Postives = 96/108 (88.89%), Query Frame = 1 
Query:    1 VGASTAYHLALRGIKSVVVERATVAAAASGKAGGFLAGGWGSGPTVRMHEVGFAMHEELAGTLGVTSYRKIPTLQVNGARKGSSPAPWLDGKASGSMMDPDTAQVRPL 324
            VGASTAYHLALRG+K +V+ER+ VAAAASGKAGGFLAGGWGSGPTV MHE+ F MHE+LA TLGVTSYRKIPTL VNG R GSSPA WLDGKASGS+MD DTAQV PL
Sbjct:   20 VGASTAYHLALRGVKPIVIERSNVAAAASGKAGGFLAGGWGSGPTVPMHEISFDMHEKLAETLGVTSYRKIPTLSVNGGRGGSSPAAWLDGKASGSLMDSDTAQVTPL 127
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BLAST of mRNA_M-pyrifera_M_contig8554.19938.1 vs. uniprot
Match: A0A835ZIK8_9STRA (FAD dependent oxidoreductase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZIK8_9STRA)

HSP 1 Score: 129 bits (325), Expect = 7.120e-34
Identity = 67/109 (61.47%), Postives = 82/109 (75.23%), Query Frame = 1 
Query:    1 VGASTAYHLALRGIKSVVVERATVAAAASGKAGGFLAGGWGSGPTVRMHEVGFAMHEELAGTLGVTSYRKIPTLQVN-GARKGSSPAPWLDGKASGSMMDPDTAQVRPL 324
            + AS AYHL  +G+K +V+ER+ VAAAASGK+GGFLA  WGSGP V++H   FA+HE+LA  L V S+RK+PTL V  G RKG + A WLDGKAS S+MD  TAQV PL
Sbjct:   19 IAASIAYHLTQKGVKPIVLERSEVAAAASGKSGGFLARDWGSGPAVQLHTASFALHEKLAKELTVESFRKLPTLSVKVGGRKGKNAASWLDGKASSSLMDDGTAQVTPL 127
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BLAST of mRNA_M-pyrifera_M_contig8554.19938.1 vs. uniprot
Match: A0A7S0QTC4_9CRYP (Hypothetical protein (Fragment) n=1 Tax=Cryptomonas curvata TaxID=233186 RepID=A0A7S0QTC4_9CRYP)

HSP 1 Score: 122 bits (306), Expect = 6.340e-33
Identity = 69/113 (61.06%), Postives = 81/113 (71.68%), Query Frame = 1 
Query:    1 VGASTAYHLALRGIKSVVVERATVAAAASGKAGGFLAGGWGSGP-TVRMHEVGFAMHEELAGTLGVTSYRKIPTLQVNGAR---KGSSPAPWLDGK-ASGSMMDPDTAQVRPL 324
            +GAS AYHL+ RG++ +VVER+ V AAASGKAGGFLAGGWG G  T ++H V F MHEELA  LG+ S+RKI TL V G R   KG  P  WLDG+ A  S+MD  TAQV PL
Sbjct:   79 IGASIAYHLSKRGVQPLVVERSKVGAAASGKAGGFLAGGWGDGSVTQQLHRVSFRMHEELAKELGIKSFRKIETLSVTGGRGGAKGKMPVSWLDGQIAKASLMDKWTAQVTPL 191
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BLAST of mRNA_M-pyrifera_M_contig8554.19938.1 vs. uniprot
Match: A0A0G4HCU1_9ALVE (DAO domain-containing protein n=1 Tax=Chromera velia CCMP2878 TaxID=1169474 RepID=A0A0G4HCU1_9ALVE)

HSP 1 Score: 125 bits (313), Expect = 7.690e-32
Identity = 68/110 (61.82%), Postives = 81/110 (73.64%), Query Frame = 1 
Query:    4 GASTAYHLALRGIKSV---VVERATVAAAASGKAGGFLAGGWGSGPTVRMHEVGFAMHEELAGTLGVTSYRKIPTLQVNGARKGSSPAPWLDGKASGSMMDPDTAQVRPL 324
            G STAYHLA +G  S+   VVER+ VAAAASGKAGGFLA  WGSGPT  +H++ F MH ELA  L + S+R I TL V+G RKG + APWL+GKAS + MD +TAQV PL
Sbjct:   67 GVSTAYHLAKKGGNSLDITVVERSGVAAAASGKAGGFLARDWGSGPTDALHKISFRMHAELAKELNIESFRPIQTLSVSGGRKGKNAAPWLNGKASSTHMDSNTAQVTPL 176
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BLAST of mRNA_M-pyrifera_M_contig8554.19938.1 vs. uniprot
Match: A0A7S0CUG7_MICPS (Hypothetical protein n=1 Tax=Micromonas pusilla TaxID=38833 RepID=A0A7S0CUG7_MICPS)

HSP 1 Score: 115 bits (287), Expect = 3.770e-28
Identity = 66/111 (59.46%), Postives = 76/111 (68.47%), Query Frame = 1 
Query:    4 GASTAYHLALRGIKSVVVERATVAAAASGKAGGFLAGGWGSGP-TVRMHEVGFAMHEELAGTLGVTSYRKIPTLQVNGARKGSS---PAPWLDGK-ASGSMMDPDTAQVRP 321
            G +TAY LA RG+ S +VER  +AAAASGKAGGFLAGGWG G  T  +H V FA+HE LA TLG+T+YRKIPTL V G    S    P  WLDG  A   +MD +TAQV P
Sbjct:   51 GCATAYFLAQRGVPSTIVERVEIAAAASGKAGGFLAGGWGDGGVTQELHRVSFALHETLAETLGLTTYRKIPTLSVAGGTSLSDQKPPVSWLDGDVARAELMDENTAQVTP 161
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BLAST of mRNA_M-pyrifera_M_contig8554.19938.1 vs. uniprot
Match: A0A7S0IJ01_9EUKA (Hypothetical protein n=1 Tax=Calcidiscus leptoporus TaxID=127549 RepID=A0A7S0IJ01_9EUKA)

HSP 1 Score: 113 bits (282), Expect = 1.120e-27
Identity = 63/112 (56.25%), Postives = 79/112 (70.54%), Query Frame = 1 
Query:    4 GASTAYHLALRGIKSVVVERATVAAAASGKAGGFLAGGWGSGPTVRMHEVGFAMHEELAGTLGVTSYRKIPTLQVNGARKGSSP---APWLDGK-ASGSMMDPDT-AQVRPL 324
            G++ AY+L  RG+K +VVE+ +VAAAASGK GGFLA  WGSGPT ++H+V +A+HEELA  LG+ SYRKIP L V   R+G       PWLDG+ A+   MD D  AQV PL
Sbjct:   17 GSALAYYLTRRGVKPIVVEQHSVAAAASGKGGGFLARDWGSGPTTQLHKVSYALHEELARELGIKSYRKIPVLSVEPGRRGKRTQDICPWLDGEIANSKYMDRDGGAQVAPL 128
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BLAST of mRNA_M-pyrifera_M_contig8554.19938.1 vs. uniprot
Match: A0A7S0EYM8_9EUKA (Hypothetical protein n=1 Tax=Phaeocystis antarctica TaxID=33657 RepID=A0A7S0EYM8_9EUKA)

HSP 1 Score: 111 bits (278), Expect = 5.410e-27
Identity = 64/112 (57.14%), Postives = 79/112 (70.54%), Query Frame = 1 
Query:    4 GASTAYHLALRGIKSVVVERATVAAAASGKAGGFLAGGWGSGPTVRMHEVGFAMHEELAGTLGVTSYRKIPTLQVNGARKGSSPA---PWLDGK-ASGSMMDPDT-AQVRPL 324
            G++ AY+L  RGIK +VVE+ +VAAAASGK GGFLA  WGSGPT ++H+V FA+H ELA TLGV SYRKIP L+V+  ++        PWLDG+ A  S MD D  AQV P 
Sbjct:   32 GSALAYYLTQRGIKPIVVEQHSVAAAASGKGGGFLARDWGSGPTTQLHKVSFALHAELAETLGVKSYRKIPVLRVSPGKRTKRTTDICPWLDGEIADSSYMDEDGGAQVAPF 143
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BLAST of mRNA_M-pyrifera_M_contig8554.19938.1 vs. uniprot
Match: A0A7S3AF44_9EUKA (Hypothetical protein n=1 Tax=Haptolina ericina TaxID=156174 RepID=A0A7S3AF44_9EUKA)

HSP 1 Score: 111 bits (277), Expect = 6.240e-27
Identity = 63/112 (56.25%), Postives = 79/112 (70.54%), Query Frame = 1 
Query:    4 GASTAYHLALRGIKSVVVERATVAAAASGKAGGFLAGGWGSGPTVRMHEVGFAMHEELAGTLGVTSYRKIPTLQV---NGARKGSSPAPWLDGKA-SGSMMDPDT-AQVRPL 324
            GA+ AY+L LRG+K ++V+R +VAAAASGK GGFLA  WGSGPTV +H+V FA+HEELA  L + SYRKIP L V      ++ +   PWLDG+    SMMD +  AQV PL
Sbjct:   18 GAALAYYLTLRGVKPLIVDRHSVAAAASGKGGGFLARDWGSGPTVALHKVSFALHEELAKELDIQSYRKIPVLSVAPGKRTKRTTDLCPWLDGEIRDSSMMDDEGGAQVAPL 129
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BLAST of mRNA_M-pyrifera_M_contig8554.19938.1 vs. uniprot
Match: A0A7S1J696_9EUGL (Hypothetical protein n=1 Tax=Eutreptiella gymnastica TaxID=73025 RepID=A0A7S1J696_9EUGL)

HSP 1 Score: 110 bits (274), Expect = 4.370e-26
Identity = 64/113 (56.64%), Postives = 75/113 (66.37%), Query Frame = 1 
Query:    1 VGASTAYHLALRGIKSVVVERATVAAAASGKAGGFLAGGWGSGPTVRMHEVGFAMHEELAGTLGVTSYRKIPTLQVNGARKGSSPAP-----WLDGK-ASGSMMDPDTAQVRP 321
            +GAS AY+L L+G K  VVER  VAAAA GK GGFLAGGWG G T ++H   F +HEELA  L + SYRKIPT+ V     GS P P     WLDG  A+  +MDP+TAQV P
Sbjct:  110 IGASIAYYLTLKGFKPTVVERKEVAAAAGGKGGGFLAGGWGYGVTEQLHTKSFELHEELARELDLKSYRKIPTISVCA---GSRPNPSKVCSWLDGNIATSRLMDPNTAQVTP 219
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BLAST of mRNA_M-pyrifera_M_contig8554.19938.1 vs. uniprot
Match: C1E2M4_MICCC (DAO domain-containing protein n=2 Tax=Micromonas TaxID=38832 RepID=C1E2M4_MICCC)

HSP 1 Score: 108 bits (270), Expect = 1.090e-25
Identity = 61/112 (54.46%), Postives = 76/112 (67.86%), Query Frame = 1 
Query:    1 VGASTAYHLALRGIKSVVVERATVAAAASGKAGGFLAGGWGSGPTVR-MHEVGFAMHEELAGTLGVTSYRKIPTLQVNGARKGSS--PAPWLDGK-ASGSMMDPDTAQVRPL 324
            +G S AY L+ RG+   VVER  +AAAASGK+GGFLAGGWG G   + +H   F +HE+LA  LG+ +YRKIPTL V G R+ ++  P  WLDG  AS  +MD  TAQV PL
Sbjct:   55 IGNSCAYFLSRRGVPCTVVERVEIAAAASGKSGGFLAGGWGDGSVTQPLHRESFRLHEQLAAELGLRTYRKIPTLSVAGGRRMAADPPVSWLDGDVASARLMDDATAQVTPL 166
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig8554.19938.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LTU1_ECTSI5.300e-5282.41DAO domain-containing protein n=2 Tax=Ectocarpus T... [more]
A0A835ZIK8_9STRA7.120e-3461.47FAD dependent oxidoreductase n=1 Tax=Tribonema min... [more]
A0A7S0QTC4_9CRYP6.340e-3361.06Hypothetical protein (Fragment) n=1 Tax=Cryptomona... [more]
A0A0G4HCU1_9ALVE7.690e-3261.82DAO domain-containing protein n=1 Tax=Chromera vel... [more]
A0A7S0CUG7_MICPS3.770e-2859.46Hypothetical protein n=1 Tax=Micromonas pusilla Ta... [more]
A0A7S0IJ01_9EUKA1.120e-2756.25Hypothetical protein n=1 Tax=Calcidiscus leptoporu... [more]
A0A7S0EYM8_9EUKA5.410e-2757.14Hypothetical protein n=1 Tax=Phaeocystis antarctic... [more]
A0A7S3AF44_9EUKA6.240e-2756.25Hypothetical protein n=1 Tax=Haptolina ericina Tax... [more]
A0A7S1J696_9EUGL4.370e-2656.64Hypothetical protein n=1 Tax=Eutreptiella gymnasti... [more]
C1E2M4_MICCC1.090e-2554.46DAO domain-containing protein n=2 Tax=Micromonas T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig8554contigM-pyrifera_M_contig8554:9298..11653 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score176.0
Seed ortholog evalue9.3e-42
Seed eggNOG ortholog2880.D8LTU1
KEGG koko:K01228
KEGG ReactionR05979
KEGG Pathwayko00510,ko01100,ko04141,map00510,map01100,map04141
KEGG ModuleM00073
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005768,GO:0005770,GO:0006810,GO:0007049,GO:0008150,GO:0008152,GO:0009987,GO:0012505,GO:0016192,GO:0016197,GO:0016482,GO:0016491,GO:0022414,GO:0031410,GO:0031982,GO:0042147,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044424,GO:0044444,GO:0044464,GO:0046907,GO:0051179,GO:0051234,GO:0051321,GO:0051641,GO:0051649,GO:0055114,GO:0097708
EggNOG free text desc.retrograde transport, endosome to Golgi
EggNOG OGsCOG0665@1,KOG2852@2759
EC3.2.1.106
COG Functional cat.E
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko04147
Exons2
Model size324
Cds size324
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816764.7923717-CDS-M-pyrifera_M_contig8554:9297..95221622816764.7923717-CDS-M-pyrifera_M_contig8554:9297..9522Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8554 9298..9522 +
1692278164.882175-CDS-M-pyrifera_M_contig8554:9297..95221692278164.882175-CDS-M-pyrifera_M_contig8554:9297..9522Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8554 9298..9522 +
1622816764.805447-CDS-M-pyrifera_M_contig8554:11554..116531622816764.805447-CDS-M-pyrifera_M_contig8554:11554..11653Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8554 11555..11653 +
1692278164.8939986-CDS-M-pyrifera_M_contig8554:11554..116531692278164.8939986-CDS-M-pyrifera_M_contig8554:11554..11653Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8554 11555..11653 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig8554.19938.1prot_M-pyrifera_M_contig8554.19938.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig8554 9298..11653 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig8554.19938.1

>prot_M-pyrifera_M_contig8554.19938.1 ID=prot_M-pyrifera_M_contig8554.19938.1|Name=mRNA_M-pyrifera_M_contig8554.19938.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=108bp
VGASTAYHLALRGIKSVVVERATVAAAASGKAGGFLAGGWGSGPTVRMHE
VGFAMHEELAGTLGVTSYRKIPTLQVNGARKGSSPAPWLDGKASGSMMDP
DTAQVRPL
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mRNA from alignment at M-pyrifera_M_contig8554:9298..11653+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig8554.19938.1 ID=mRNA_M-pyrifera_M_contig8554.19938.1|Name=mRNA_M-pyrifera_M_contig8554.19938.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=2356bp|location=Sequence derived from alignment at M-pyrifera_M_contig8554:9298..11653+ (Macrocystis pyrifera P11B4 male)
GTCGGCGCAAGCACCGCATACCACCTGGCACTACGGGGCATCAAGTCGGT
CGTGGTCGAGCGCGCTACCGTCGCCGCCGCCGCAAGCGGTAAAGCGGGAG
GGTTTTTGGCTGGGGGTTGGGGTAGCGGGCCGACCGTGCGCATGCACGAG
GTCGGCTTCGCGATGCACGAGGAGCTGGCCGGCACGCTGGGAGTGACTTC
GTACCGCAAGATTCCTACTCTCCAGGTGGGTCCTTGTTTTAGACGTGGCT
GTTGTCGTTGCCCGTCACACAAGTGCCCGGGACCCCCCCCCCACCTTGCG
TAAGGTGGACGTAAAACAATCCCTTGTGGAGATTTTGAGCAATGTCTATA
GCAAAACCCACAACGCGTGGACAACATTTAGATTGGTACGCCTAATGGGG
TGCTACTGTTTTCAACGTCTGCACAACGATGTTCTTGTGCCCACGTTGTG
CAGGGGGAGGGTCCCGGCCACCTCTGCAGGGTGGACGTTGTCGTCTGCGT
CGGTGATGTCGGTGTTGCACGGGTGGACAACCCCGCCCAAGGCATGCCTT
GGTGCTAGCCGGGGTCGAAAAGCCATACCATGCATTGAGGACATGTTGGT
ATGAAAATCACAGCAAAGTGGCGTCCAAACGTACGTACGTACGTACAGTC
TCAAGATTGAGTGGATGCTGTGACGATTTTCAGCTTGACAACGTGTCCTC
GATGTGTTGCGCAATGGTTCGGCTCTCGATGTTGGGTTTCGTACCGGGTG
GACCTGTACACAGGTGTGTCGTCGAGGTGTCGTCGTTGTTGTCGCGGTCG
GTATTGTAGCCGTAGTCCTTGTTGGCGATGTAGCATTAGTGTGAAACGGT
TTCCACCATGGGAGGCGGTGTCGACAGGAAGCGAGATAGTGTTACACAAG
TCGCTCTTTTTTTGTTTTGTTCTTTTTTATCACCGGTGCCGTCGTCATCA
TTGCCGCTGCCATCGTCAGCATCGGCGTCATTACCATGGTTGTTGATGGT
GATGTGGGGGTCCGTGTCAAACGTTTGCATGGCATCCGGCGGTTCCGGCA
GCAAAACGAGACGACTGTGACGCGCAACTCGCTCCGGTTTTGTAACCGTC
CGTCACTGTCGTGGTCACGCTTGTCTCCGTCGACTCCATTGCCGTTGACG
ACGTCATCGTGTCGGCATTGCCGCCGTCGCTGTCGTCATCGGTGGTGATG
GTGTGGGGCCAGTGTCGAACGGTTCTAGAGGGCTGTATCGTCAGGAAACG
AGGCAATGCGGCACAACTCGCTTCCTTTTTGTAACCGTCGTCCCTGTCAT
CGTCACGTTTGTCGCCGTCGACATTACTGTAATTTGTCGGTTTTGTCGTC
ATTGCTGTTGCCTTTTGGCGTTGTCGTCGTCGCTGAGGAGGTTGTGGGCC
CGGTGTCGAACGGTTCTAGGAGGTAGTCTCGTCAAGAAAACGAGACGACA
ACGACGCACAGATCGCTCCAGTTTTGTAACCGTCACCATTCTCGTTGTCA
TCGAGGATGTGTGTGGGACTCTTGTCGTCAAGAACCTTGGCAAAAGCGCA
CAACTCGCTTGTGCTGGGTCATCGTCGTCGCCGTTGTCGTCGTTGTAGTC
CATGATGGTTATCGCTTTTAGAGCTGCAGTCCCGTTTTGGAGTCAAACTA
CTTGGAATTTGAGTGGTTTGCCCCTTGAGCGGGACTGAAGTCCTAAAAGG
GGGTAATCGGCGATATGAAACCGTTCTGCAGCTGTATTGGTAGGCGTTAT
TGTCCGCGGAAAGCAGGGCAAGATTGTAGCGCGCTACTCGCACCTCTCGC
GGTAGCCAATGCCGTCATCTTTGTGTCGTGGATATTTTACCGTTGTCTTT
GATGGTAATGTGGGGGCGGCTTCGAATGGTTCGATGTGGTAAGCTCGGCA
ACAAGCGCGGAAAATTCGCGCCTGAATCACACATCGGTCGCATGCTTGTT
CAGGTTTCAACACCCTCAGGCAGGCATTGGGCTGCCGTGCCCAGGTGGTT
GTTTTATCCTTTTTCGGGCAAGGAACGAGGCGTGCAGGACGTGATGCGCA
GGGGTGTCCTGTCCTTCTCGGATTAGTGCTACGTTTTTGTAGTATGCGCC
TCCCTCCCCCCCGTCCTCTCTCCACCCCTTCCCCATCCCACCCGACCCCC
CTCCTCTCTCCACCCCTTCCCTACCCCACTCGACCCCACGCCACCCCACC
CCATTCCTTGTCGCGGTTGTGATTGTCGCTTGTGTCGGGCCTGGTTTTGA
CACAAAGGTGAACGGGGCACGGAAAGGATCCTCACCGGCACCTTGGCTGG
ATGGGAAGGCTTCGGGGTCGATGATGGATCCCGACACAGCTCAGGTGCGA
CCATTG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig8554:9298..11653+

>mRNA_M-pyrifera_M_contig8554.19938.1 ID=mRNA_M-pyrifera_M_contig8554.19938.1|Name=mRNA_M-pyrifera_M_contig8554.19938.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=648bp|location=Sequence derived from alignment at M-pyrifera_M_contig8554:9298..11653+ (Macrocystis pyrifera P11B4 male)
GTCGGCGCAAGCACCGCATACCACCTGGCACTACGGGGCATCAAGTCGGT
CGTGGTCGAGCGCGCTACCGTCGCCGCCGCCGCAAGCGGTAAAGCGGGAG
GGTTTTTGGCTGGGGGTTGGGGTAGCGGGCCGACCGTGCGCATGCACGAG
GTCGGCTTCGCGATGCACGAGGAGCTGGCCGGCACGCTGGGAGTGACTTC
GTACCGCAAGATTCCTACTCTCCAGGTCGGCGCAAGCACCGCATACCACC
TGGCACTACGGGGCATCAAGTCGGTCGTGGTCGAGCGCGCTACCGTCGCC
GCCGCCGCAAGCGGTAAAGCGGGAGGGTTTTTGGCTGGGGGTTGGGGTAG
CGGGCCGACCGTGCGCATGCACGAGGTCGGCTTCGCGATGCACGAGGAGC
TGGCCGGCACGCTGGGAGTGACTTCGTACCGCAAGATTCCTACTCTCCAG
GTGAACGGGGCACGGAAAGGATCCTCACCGGCACCTTGGCTGGATGGGAA
GGCTTCGGGGTCGATGATGGATCCCGACACAGCTCAGGTGCGACCATTGG
TGAACGGGGCACGGAAAGGATCCTCACCGGCACCTTGGCTGGATGGGAAG
GCTTCGGGGTCGATGATGGATCCCGACACAGCTCAGGTGCGACCATTG
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