mRNA_M-pyrifera_M_contig79133.18579.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig79133.18579.1
Unique NamemRNA_M-pyrifera_M_contig79133.18579.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig79133.18579.1 vs. uniprot
Match: A0A3D5DBR8_9GAMM (Ligand-gated channel (Fragment) n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A3D5DBR8_9GAMM)

HSP 1 Score: 220 bits (560), Expect = 4.390e-67
Identity = 110/195 (56.41%), Postives = 134/195 (68.72%), Query Frame = 1
Query:    1 FINDISDFTFLRLNDRNGDGVADRVEDDFGVTGMIVDEDDALLLVNQAQADAFFWGLELEAEATVFNNDYGLLDLRLWTDRVSGEFDDGANVPRLPPPRFGGALNWSRGAYYAQLAATRTTEQDELAALETRTGAFTELNVQAGYRAQVGKTE-LSLFARGSNLLDETQRRHVSLVKDLAPRPGIAGLIGVRLRY 582
            F ND+SDF FL+  D N DG ADRVE +F  TG IVDEDDALLLVN  Q DA FWG E+E   T+ ++  G ++LR W+D V GE D G NVPRLPP RFGG L W RG  YA ++ TRTT+QD+ AALET T  ++ +N+ AGY   VG    ++ FARG+NL +E  RRH S VK LAP PGI+GL G+R  Y
Sbjct:  226 FYNDVSDFVFLQSGDSNADGNADRVESNFLQTGEIVDEDDALLLVNSVQTDAEFWGFEVETRMTLIDDSRGSVELRAWSDYVDGELDSGGNVPRLPPLRFGGGLEWERGPAYAGVSVTRTTKQDDAAALETDTDGYSMVNIHAGYTVPVGNNRSITFFARGTNLANEDARRHTSFVKHLAPLPGISGLFGIRASY 420          
BLAST of mRNA_M-pyrifera_M_contig79133.18579.1 vs. uniprot
Match: D5BWN4_NITHN (TonB-dependent receptor n=4 Tax=Nitrosococcus TaxID=1227 RepID=D5BWN4_NITHN)

HSP 1 Score: 176 bits (447), Expect = 2.400e-48
Identity = 90/194 (46.39%), Postives = 128/194 (65.98%), Query Frame = 1
Query:    1 FINDISDFTFLRLNDRNGDGVADRVEDDFGVTGMIVDEDDALLLVNQAQADAFFWGLELEAEATVFNNDYGLLDLRLWTDRVSGEFDDGANVPRLPPPRFGGALNWSRGAYYAQLAATRTTEQDELAALETRTGAFTELNVQAGYRAQVGKTELSLFARGSNLLDETQRRHVSLVKDLAPRPGIAGLIGVRLRY 582
            F N I+DF FL+  D+NGDGVADRV     + G +V + +ALLLVNQ QADA F+G+E E    +F++  G LDLRLWTD VSGE  +G+N+PR+ P RFG  L++ RG +Y  +   R   Q++ A LET T  ++ L+V  GY+  +G  + ++F RG+NLLDE  RRH S +K+ AP PG + ++G+   +
Sbjct:  503 FANFINDFIFLQERDQNGDGVADRVN----LEGELVIDPEALLLVNQRQADAEFFGVEFETVLGLFDDHRGKLDLRLWTDYVSGELSEGSNLPRITPLRFGSELDYERGPWYTGINIMRVQRQEDTALLETETKGYSMLSVHMGYQLALGPVQYTIFLRGTNLLDEEARRHTSFLKNQAPLPGRSAMVGITATF 692          
BLAST of mRNA_M-pyrifera_M_contig79133.18579.1 vs. uniprot
Match: Q3JB21_NITOC (TonB-dependent receptor n=7 Tax=Nitrosococcus TaxID=1227 RepID=Q3JB21_NITOC)

HSP 1 Score: 176 bits (445), Expect = 4.470e-48
Identity = 94/194 (48.45%), Postives = 124/194 (63.92%), Query Frame = 1
Query:    1 FINDISDFTFLRLNDRNGDGVADRVEDDFGVTGMIVDEDDALLLVNQAQADAFFWGLELEAEATVFNNDYGLLDLRLWTDRVSGEFDDGANVPRLPPPRFGGALNWSRGAYYAQLAATRTTEQDELAALETRTGAFTELNVQAGYRAQVGKTELSLFARGSNLLDETQRRHVSLVKDLAPRPGIAGLIGVRLRY 582
            F N ISDF FL+  D+NGDG+ADRV     + G    + D LLLVN+ QADA F+G+E E    +F +  G LDLRLWTD V G+  +GAN+PR+ P RFG  L++ RG  YA +   R   QDE AALET TG +T L V  GYR  +G  + ++F RG+NLL+E  RRH S +K+ AP PG + ++GV   +
Sbjct:  500 FANFISDFIFLQEQDKNGDGIADRVN----LEGEPSMDPDGLLLVNEKQADAEFFGVEFETVIGLFEDHRGKLDLRLWTDYVRGKLKNGANLPRITPLRFGSELDYERGPGYAGINLMRVQAQDETAALETETGGYTLLKVYMGYRFALGPVQYAVFLRGTNLLNEKARRHTSFLKNQAPLPGRSAMVGVNATF 689          
BLAST of mRNA_M-pyrifera_M_contig79133.18579.1 vs. uniprot
Match: A0A6L8EJ60_9GAMM (TonB-dependent receptor n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A6L8EJ60_9GAMM)

HSP 1 Score: 155 bits (392), Expect = 1.950e-40
Identity = 91/199 (45.73%), Postives = 118/199 (59.30%), Query Frame = 1
Query:    1 FINDISDFTFLRLNDRNGDGVADRVEDDFG-----VTGMIVDEDDALLLVNQAQADAFFWGLELEAEATVFNNDYGLLDLRLWTDRVSGEFDDGANVPRLPPPRFGGALNWSRGAYYAQLAATRTTEQDELAALETRTGAFTELNVQAGYRAQVGKTELSLFARGSNLLDETQRRHVSLVKDLAPRPGIAGLIGVRLRY 582
            F N I D+ + +  D NGDG ADRV + FG     +    VDE+  L LV  +Q DA F G E E  A + +++ G + LRLW D V GE  +  N+ R+ P RFG  L +SRG  YA +  TR  +QD  A LET T  +  L+V A Y  + G   ++LFARGSNLLDE  RRHVS VKD AP PG +G+ GVR+ +
Sbjct:  576 FYNRIDDYIYYQEQDLNGDGFADRVTEFFGGNPANILAPGVDEE--LFLVYISQDDAEFLGFEAETRARLMDDNNGRMVLRLWADYVEGERSNNTNLARITPWRFGSGLTYSRGDLYAAVNYTRVNKQDSTAPLETETDGYHLLDVHAEYTFRTGGNRVTLFARGSNLLDEEIRRHVSFVKDQAPLPGRSGIFGVRVGF 772          
BLAST of mRNA_M-pyrifera_M_contig79133.18579.1 vs. uniprot
Match: A4BUR0_9GAMM (TonB-dependent receptor n=1 Tax=Nitrococcus mobilis Nb-231 TaxID=314278 RepID=A4BUR0_9GAMM)

HSP 1 Score: 148 bits (373), Expect = 4.880e-38
Identity = 83/194 (42.78%), Postives = 114/194 (58.76%), Query Frame = 1
Query:    1 FINDISDFTFLRLNDRNGDGVADRVEDDFGVTGMIVDEDDALLLVNQAQADAFFWGLELEAEATVFNNDYGLLDLRLWTDRVSGEFDDGANVPRLPPPRFGGALNWSRGAYYAQLAATRTTEQDELAALETRTGAFTELNVQAGYRAQVGKTELSLFARGSNLLDETQRRHVSLVKDLAPRPGIAGLIGVRLRY 582
            F+N I +F F +  DR+GDG ADRV+D+    G        LLLV+  Q DA F+G E+EA   +F+N  G LD RL+ D V G+   G ++PR+ P R G  L++ RG   A L  T+   Q+  A LETRTG +T L++   Y   V   E  +F RG NLLDET RRH S +KD AP  G + ++GV +++
Sbjct:  479 FVNLIDNFIFAQSQDRDGDGSADRVDDEGNPGGN-------LLLVDYTQTDALFFGAEMEALYGLFDNANGTLDARLFGDWVRGQLSGGPDLPRISPARLGFGLDYHRGPLEANLETTQVFTQNATAPLETRTGGYTMLDLGLSYTLYVDPLETKVFLRGRNLLDETARRHNSFIKDRAPLQGRSAIVGVNIQF 665          
BLAST of mRNA_M-pyrifera_M_contig79133.18579.1 vs. uniprot
Match: A0A259EY51_9PROT (TonB-dependent receptor n=1 Tax=Hydrogenophilales bacterium 17-61-9 TaxID=1970524 RepID=A0A259EY51_9PROT)

HSP 1 Score: 137 bits (345), Expect = 3.870e-34
Identity = 85/195 (43.59%), Postives = 113/195 (57.95%), Query Frame = 1
Query:    1 FINDISDFTFLRLNDRNGDGVADRVEDDFGVTGMIVDEDDALLLVNQAQADAFFWGLELEAEATVFNNDYGLLDLRLWTDRVSGEFDDGANVPRLPPPRFGGALNWSRGAYYAQLAATRTTEQDELAALETRTGAFTELNVQAGYRAQVGKT-ELSLFARGSNLLDETQRRHVSLVKDLAPRPGIAGLIGVRLRY 582
            F N I ++ F R  D +GDGVADRV+D        +D D   L+ N AQ  A F+G E EA  T+     G LDLRL+ D V G+ D G NVPR PP RFG  L+   GA+ A L+  R   Q+ LA LET T A+T  N +  YR +  K+   ++F +G NLLD   R H S +KD+AP PG A ++G+R ++
Sbjct:  491 FHNRIGNYIFERSADTDGDGVADRVDD-----AGTLDPDGEYLVQNVAQTGARFYGAEAEAVFTLLP---GALDLRLFADTVRGKLDRGGNVPRTPPHRFGLQLDHQAGAWAANLSVVRAARQNRLAELETATPAYTLANAEVSYRIKQDKSIGYTVFVQGKNLLDNEIRVHTSYLKDVAPLPGRAFVLGLRGQF 677          
BLAST of mRNA_M-pyrifera_M_contig79133.18579.1 vs. uniprot
Match: F8GGH8_NITSI (TonB-dependent receptor n=1 Tax=Nitrosomonas sp. (strain Is79A3) TaxID=261292 RepID=F8GGH8_NITSI)

HSP 1 Score: 135 bits (340), Expect = 1.920e-33
Identity = 79/192 (41.15%), Postives = 112/192 (58.33%), Query Frame = 1
Query:    1 FINDISDFTFLRLNDRNGDGVADRVEDDFGVTGMIVDEDDALLLVNQAQADAFFWGLELEAEATVFNNDYGLLDLRLWTDRVSGEFDDGANVPRLPPPRFGGALNWSRGAYYAQLAATRTTEQDELAALETRTGAFTELNVQAGYRAQVGKT-ELSLFARGSNLLDETQRRHVSLVKDLAPRPGIAGLIGVR 573
            F N ++D+ F +  D NGDG ADRV D+      ++D+  A L+ + +Q  A F+GLE EA   +  +    L+LRL+TD V G   D  N+PRL P RFG  L++ + A+ A    TR T QD +A LET T  +T +N + GY  ++ K+   +LF +G NLLD   R H S +KD AP PG A + G+R
Sbjct:  497 FYNYVNDYIFQQSRDSNGDGFADRVNDE-----GLLDKHGAFLVQDFSQTRAKFYGLEAEAIIALLPD---TLNLRLFTDIVYGRLKDNGNIPRLTPQRFGFDLDYRKNAWQANFNLTRVTRQDRVAVLETETSGYTLMNAEMGYHMKLAKSVHYTLFLQGRNLLDSDMRVHTSFLKDAAPLPGRAIVAGIR 680          
BLAST of mRNA_M-pyrifera_M_contig79133.18579.1 vs. uniprot
Match: A0A2V3W6T8_9PROT (Iron complex outermembrane receptor protein n=1 Tax=Nitrosomonas sp. Nm84 TaxID=200124 RepID=A0A2V3W6T8_9PROT)

HSP 1 Score: 135 bits (339), Expect = 2.630e-33
Identity = 79/192 (41.15%), Postives = 113/192 (58.85%), Query Frame = 1
Query:    1 FINDISDFTFLRLNDRNGDGVADRVEDDFGVTGMIVDEDDALLLVNQAQADAFFWGLELEAEATVFNNDYGLLDLRLWTDRVSGEFDDGANVPRLPPPRFGGALNWSRGAYYAQLAATRTTEQDELAALETRTGAFTELNVQAGYRAQVGKT-ELSLFARGSNLLDETQRRHVSLVKDLAPRPGIAGLIGVR 573
            F N ++D+ F +  D NGDG ADRV D+      ++D   A L+ + AQ  A F+GLE EA  T+      +L+LRL+TD V G   D  N+PRL P RFG  +++ + A+ A    TR  +QD +A LET T  +T +N + GY  ++ K+   +LF +G NLLD   R H S +KD+AP PG A + G+R
Sbjct:  497 FYNYVNDYIFQQSRDSNGDGFADRVNDE-----GVLDSRGAFLVQDFAQTRAKFYGLEAEAIVTLLPE---MLNLRLFTDIVYGRLKDNGNIPRLTPQRFGFDVDFKKNAWSANFNLTRVVQQDRVAVLETETPGYTLMNAEMGYHMKLTKSVNYTLFLQGRNLLDSDMRVHTSFLKDIAPLPGRAIVGGIR 680          
BLAST of mRNA_M-pyrifera_M_contig79133.18579.1 vs. uniprot
Match: A0A286C0N8_9PROT (Iron complex outermembrane recepter protein n=2 Tax=unclassified Nitrosovibrio TaxID=2624428 RepID=A0A286C0N8_9PROT)

HSP 1 Score: 134 bits (336), Expect = 6.820e-33
Identity = 82/192 (42.71%), Postives = 116/192 (60.42%), Query Frame = 1
Query:    1 FINDISDFTFLRLNDRNGDGVADRVEDDFGVTGMIVDEDDALLLVNQAQADAFFWGLELEAEATVFNNDYGLLDLRLWTDRVSGEFDDGANVPRLPPPRFGGALNWSRGAYYAQLAATRTTEQDELAALETRTGAFTELNVQAGYRAQVGKTE-LSLFARGSNLLDETQRRHVSLVKDLAPRPGIAGLIGVR 573
            F N I ++ F+R  D NGDGVADR +D    TG + D D   L+ N AQ  A F+G E EA  T+  ++   +DLRL+ D V G  D+G NVPR+ P RFG   N   G + + ++ATR   Q++LA LET T  +T +NV+  YR +  ++  + +F +G NLLDE  R H S +K+ AP PG A ++G+R
Sbjct:  499 FHNWIGNYIFVRSADINGDGVADRADD----TGTL-DPDGEFLVQNFAQGGARFYGAEAEAIFTLKPDE---IDLRLFADYVRGRLDNGGNVPRITPLRFGLEFNHRTGPWTSNVSATRVMRQNDLAELETSTPGYTLVNVEVSYRIKETRSNGIKIFLQGKNLLDEEMRVHNSFLKNFAPLPGRALVVGLR 682          
BLAST of mRNA_M-pyrifera_M_contig79133.18579.1 vs. uniprot
Match: A0A358JL75_9PROT (TonB-dependent receptor (Fragment) n=1 Tax=Nitrosomonas sp. TaxID=42353 RepID=A0A358JL75_9PROT)

HSP 1 Score: 132 bits (331), Expect = 9.580e-33
Identity = 79/192 (41.15%), Postives = 113/192 (58.85%), Query Frame = 1
Query:    1 FINDISDFTFLRLNDRNGDGVADRVEDDFGVTGMIVDEDDALLLVNQAQADAFFWGLELEAEATVFNNDYGLLDLRLWTDRVSGEFDDGANVPRLPPPRFGGALNWSRGAYYAQLAATRTTEQDELAALETRTGAFTELNVQAGYRAQVGKTEL-SLFARGSNLLDETQRRHVSLVKDLAPRPGIAGLIGVR 573
            F N I D+ F R  D NGDG+ADRV D+    G + D +   L+ + AQ  A F+GLE EA   +       L++RL+TD V G   +  N+PRL P RFG  +++ +G +      TRTT Q+ +A LET T  +T +N +  YR + G++   +LF +G NLLDE  R H S +KD+AP PG A ++G+R
Sbjct:  312 FYNHIHDYIFQRNRDSNGDGLADRVNDE----GEL-DINGPFLVQDFAQTRAHFYGLEAEARIILLPE---RLNMRLFTDIVYGRLKNNGNIPRLTPQRFGWEMDYFQGNWQGNFNVTRTTRQNRVATLETETPGYTLMNAELSYRFKRGESAYHTLFLQGRNLLDEDMRVHTSFLKDVAPLPGRAIVVGIR 495          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig79133.18579.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3D5DBR8_9GAMM4.390e-6756.41Ligand-gated channel (Fragment) n=1 Tax=Gammaprote... [more]
D5BWN4_NITHN2.400e-4846.39TonB-dependent receptor n=4 Tax=Nitrosococcus TaxI... [more]
Q3JB21_NITOC4.470e-4848.45TonB-dependent receptor n=7 Tax=Nitrosococcus TaxI... [more]
A0A6L8EJ60_9GAMM1.950e-4045.73TonB-dependent receptor n=1 Tax=Gammaproteobacteri... [more]
A4BUR0_9GAMM4.880e-3842.78TonB-dependent receptor n=1 Tax=Nitrococcus mobili... [more]
A0A259EY51_9PROT3.870e-3443.59TonB-dependent receptor n=1 Tax=Hydrogenophilales ... [more]
F8GGH8_NITSI1.920e-3341.15TonB-dependent receptor n=1 Tax=Nitrosomonas sp. (... [more]
A0A2V3W6T8_9PROT2.630e-3341.15Iron complex outermembrane receptor protein n=1 Ta... [more]
A0A286C0N8_9PROT6.820e-3342.71Iron complex outermembrane recepter protein n=2 Ta... [more]
A0A358JL75_9PROT9.580e-3341.15TonB-dependent receptor (Fragment) n=1 Tax=Nitroso... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig79133contigM-pyrifera_M_contig79133:384..968 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score179.1
Seed ortholog evalue2e-42
Seed eggNOG ortholog105559.Nwat_1623
Preferred namephuR
KEGG koko:K02014
KEGG TC1.B.14
Hectar predicted targeting categoryother localisation
EggNOG free text desc.TonB-dependent Receptor Plug
EggNOG OGs1MUZG@1224,1RP7I@1236,1WXPY@135613,COG4206@1,COG4206@2
COG Functional cat.H
Best tax levelChromatiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko02000
Exons1
Model size585
Cds size585
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816694.3595815-CDS-M-pyrifera_M_contig79133:383..9681622816694.3595815-CDS-M-pyrifera_M_contig79133:383..968Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig79133 384..968 -
1692278123.977465-CDS-M-pyrifera_M_contig79133:383..9681692278123.977465-CDS-M-pyrifera_M_contig79133:383..968Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig79133 384..968 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig79133.18579.1prot_M-pyrifera_M_contig79133.18579.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig79133 384..968 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig79133.18579.1

>prot_M-pyrifera_M_contig79133.18579.1 ID=prot_M-pyrifera_M_contig79133.18579.1|Name=mRNA_M-pyrifera_M_contig79133.18579.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=195bp
FINDISDFTFLRLNDRNGDGVADRVEDDFGVTGMIVDEDDALLLVNQAQA
DAFFWGLELEAEATVFNNDYGLLDLRLWTDRVSGEFDDGANVPRLPPPRF
GGALNWSRGAYYAQLAATRTTEQDELAALETRTGAFTELNVQAGYRAQVG
KTELSLFARGSNLLDETQRRHVSLVKDLAPRPGIAGLIGVRLRY*
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mRNA from alignment at M-pyrifera_M_contig79133:384..968-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig79133.18579.1 ID=mRNA_M-pyrifera_M_contig79133.18579.1|Name=mRNA_M-pyrifera_M_contig79133.18579.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=585bp|location=Sequence derived from alignment at M-pyrifera_M_contig79133:384..968- (Macrocystis pyrifera P11B4 male)
TTCATCAACGACATCTCGGACTTTACGTTTTTGCGCCTGAACGATCGCAA CGGCGACGGGGTCGCCGACCGGGTGGAAGATGATTTCGGTGTGACGGGCA TGATCGTCGATGAGGACGACGCCCTGTTGCTCGTCAACCAGGCGCAGGCG GACGCGTTCTTCTGGGGCCTCGAGCTCGAGGCCGAAGCGACGGTATTCAA CAACGACTACGGGCTCCTGGATTTGCGTCTGTGGACGGATCGGGTCAGCG GTGAATTCGACGACGGTGCCAACGTTCCCCGGCTCCCGCCGCCGCGTTTC GGTGGGGCGCTCAACTGGTCGCGTGGCGCCTACTATGCGCAGCTCGCGGC CACGCGCACGACGGAGCAGGATGAACTCGCGGCGCTCGAAACCCGCACCG GTGCGTTCACGGAGCTGAACGTACAGGCCGGTTATCGCGCGCAGGTGGGC AAAACGGAATTGTCGCTGTTCGCGCGCGGTTCAAACCTGCTTGACGAAAC GCAGCGCCGCCACGTTTCGCTGGTGAAAGACCTCGCGCCGCGGCCGGGAA TAGCGGGTCTGATCGGCGTACGGCTGCGCTACTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig79133:384..968-

>mRNA_M-pyrifera_M_contig79133.18579.1 ID=mRNA_M-pyrifera_M_contig79133.18579.1|Name=mRNA_M-pyrifera_M_contig79133.18579.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1170bp|location=Sequence derived from alignment at M-pyrifera_M_contig79133:384..968- (Macrocystis pyrifera P11B4 male)
TTCATCAACGACATCTCGGACTTTACGTTTTTGCGCCTGAACGATCGCAA
CGGCGACGGGGTCGCCGACCGGGTGGAAGATGATTTCGGTGTGACGGGCA
TGATCGTCGATGAGGACGACGCCCTGTTGCTCGTCAACCAGGCGCAGGCG
GACGCGTTCTTCTGGGGCCTCGAGCTCGAGGCCGAAGCGACGGTATTCAA
CAACGACTACGGGCTCCTGGATTTGCGTCTGTGGACGGATCGGGTCAGCG
GTGAATTCGACGACGGTGCCAACGTTCCCCGGCTCCCGCCGCCGCGTTTC
GGTGGGGCGCTCAACTGGTCGCGTGGCGCCTACTATGCGCAGCTCGCGGC
CACGCGCACGACGGAGCAGGATGAACTCGCGGCGCTCGAAACCCGCACCG
GTGCGTTCACGGAGCTGAACGTACAGGCCGGTTATCGCGCGCAGGTGGGC
AAAACGGAATTGTCGCTGTTCGCGCGCGGTTCAAACCTGCTTGACGAAAC
GCAGCGCCGCCACGTTTCGCTGGTGAAAGACCTCGCGCCGCGGCCGGGAA
TAGCGGGTCTGATCGGCGTACGGCTGCGCTACTAGTTCATCAACGACATC
TCGGACTTTACGTTTTTGCGCCTGAACGATCGCAACGGCGACGGGGTCGC
CGACCGGGTGGAAGATGATTTCGGTGTGACGGGCATGATCGTCGATGAGG
ACGACGCCCTGTTGCTCGTCAACCAGGCGCAGGCGGACGCGTTCTTCTGG
GGCCTCGAGCTCGAGGCCGAAGCGACGGTATTCAACAACGACTACGGGCT
CCTGGATTTGCGTCTGTGGACGGATCGGGTCAGCGGTGAATTCGACGACG
GTGCCAACGTTCCCCGGCTCCCGCCGCCGCGTTTCGGTGGGGCGCTCAAC
TGGTCGCGTGGCGCCTACTATGCGCAGCTCGCGGCCACGCGCACGACGGA
GCAGGATGAACTCGCGGCGCTCGAAACCCGCACCGGTGCGTTCACGGAGC
TGAACGTACAGGCCGGTTATCGCGCGCAGGTGGGCAAAACGGAATTGTCG
CTGTTCGCGCGCGGTTCAAACCTGCTTGACGAAACGCAGCGCCGCCACGT
TTCGCTGGTGAAAGACCTCGCGCCGCGGCCGGGAATAGCGGGTCTGATCG
GCGTACGGCTGCGCTACTAG
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