mRNA_M-pyrifera_M_contig77478.18240.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig77478.18240.1
Unique NamemRNA_M-pyrifera_M_contig77478.18240.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig77478.18240.1 vs. uniprot
Match: A0A4D9CTT8_9STRA (Thioredoxin domain-containing protein n=1 Tax=Nannochloropsis salina CCMP1776 TaxID=1027361 RepID=A0A4D9CTT8_9STRA)

HSP 1 Score: 148 bits (374), Expect = 3.020e-42
Identity = 79/141 (56.03%), Postives = 99/141 (70.21%), Query Frame = 1
Query:   31 AAILGAVGSWGLYTPGRTAVTLISSKKEFAQEVTKFDGVALVEFYAPWCGHCKNLVPEWEKAAKALKGVAKVVAVDATEHAELASKYGVRGYPTIKVFGADKRSPSDYQGGRTAKEIVSGAKKAIGELLGKRMGGSGGSKG 453
            AA+L  + +  LY+     V L  +++ F ++V K +GV LVEFYA WCGHCKNLVPEWEKAA+ALKGV  V AVDA+ H  L  KY V+G+PTIKVFG DKR P DYQGGRTAK+IV+    A   L+ +R+ G GG +G
Sbjct:   21 AAVLLPLPTKALYSARSDVVQL--TEENFKEKVLKAEGVVLVEFYAAWCGHCKNLVPEWEKAAQALKGVVTVAAVDASVHQGLGQKYDVKGFPTIKVFGGDKRKPGDYQGGRTAKDIVNEGLGAARALVKERLNGGGGREG 159          
BLAST of mRNA_M-pyrifera_M_contig77478.18240.1 vs. uniprot
Match: A0A8J5XM45_DIALT (Uncharacterized protein n=1 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J5XM45_DIALT)

HSP 1 Score: 152 bits (383), Expect = 3.100e-41
Identity = 78/135 (57.78%), Postives = 91/135 (67.41%), Query Frame = 1
Query:   40 LGAVGSWGLYTPGRTAVTLISSKKEFAQEVTKFDGVALVEFYAPWCGHCKNLVPEWEKAAKALKGVAKVVAVDATEHAELASKYGVRGYPTIKVFGADKRSPSDYQGGRTAKEIVSGAKKAIGELLGKRMGGSGG 444
            L +VG+       R  V L+     F +EV  FDGV  VEFYAPWCGHCK+LVP+W +AAKALKGV KV AVDAT    LA KYG++G+PTIK+FGADKR PSDY G R A  IV     A  EL+G R+GG GG
Sbjct:   10 LASVGAASALYSARDGVVLVDGAS-FQKEVLDFDGVVAVEFYAPWCGHCKSLVPDWARAAKALKGVVKVAAVDATAEEALARKYGIKGFPTIKLFGADKRRPSDYGGARDAPSIVQSCLNAARELVGSRIGGQGG 143          
BLAST of mRNA_M-pyrifera_M_contig77478.18240.1 vs. uniprot
Match: A0A6V1Q1R9_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A6V1Q1R9_HETAK)

HSP 1 Score: 148 bits (374), Expect = 6.920e-40
Identity = 71/99 (71.72%), Postives = 79/99 (79.80%), Query Frame = 1
Query:   88 VTLISSKKEFAQEVTKFDGVALVEFYAPWCGHCKNLVPEWEKAAKALKGVAKVVAVDATEHAELASKYGVRGYPTIKVFGADKRSPSDYQGGRTAKEIV 384
            V  IS   +F ++V K DGV LVEFYAPWCGHC+NLVPEWEKAAK LKGV  V AVDAT+   LA KYGV+GYPTIKVFGADKR+P+DYQG RTA  IV
Sbjct:   30 VEQISGNVDFREKVLKSDGVWLVEFYAPWCGHCRNLVPEWEKAAKILKGVVNVAAVDATQDQSLAQKYGVQGYPTIKVFGADKRNPTDYQGQRTADAIV 128          
BLAST of mRNA_M-pyrifera_M_contig77478.18240.1 vs. uniprot
Match: UPI001C08AA9B (protein disulfide-isomerase A6 n=2 Tax=Alosa TaxID=34772 RepID=UPI001C08AA9B)

HSP 1 Score: 148 bits (374), Expect = 7.040e-40
Identity = 84/151 (55.63%), Postives = 106/151 (70.20%), Query Frame = 1
Query:    4 MRSI--VVLASAAILGAVGSWGLYTPGRTAVTLISSKKEFAQEVTKFDGVALVEFYAPWCGHCKNLVPEWEKAAKALKGVAKVVAVDATEHAELASKYGVRGYPTIKVFGADKRSPSDYQGGRTAKEIVSGAKKAIGELLGKRMGG-SGGS 447
            MRS+  V+L S A+L      GLY+P    V L  S   F +EV + D + LVEFYAPWCGHCKNLVP+W+KAA ALKG+ KV AVDA +H  L  +YGVRG+PTIKVFGA+K  P+DYQGGR+++ IV  A  A+  L+  R+GG +GGS
Sbjct:    1 MRSVLGVLLCSWAVLSV---HGLYSPSDDVVELTPSN--FNREVLQSDSLWLVEFYAPWCGHCKNLVPDWKKAATALKGIVKVGAVDADQHKSLGGQYGVRGFPTIKVFGANKNKPADYQGGRSSQAIVDEAINALRSLVKDRLGGRTGGS 146          
BLAST of mRNA_M-pyrifera_M_contig77478.18240.1 vs. uniprot
Match: A0A5A8DQB4_CAFRO (Uncharacterized protein n=2 Tax=Sar TaxID=2698737 RepID=A0A5A8DQB4_CAFRO)

HSP 1 Score: 148 bits (374), Expect = 7.660e-40
Identity = 78/141 (55.32%), Postives = 99/141 (70.21%), Query Frame = 1
Query:   19 VLASAAILGAVG---SWGLYTPGRTAVTLISSKKEFAQEVTKFDGVALVEFYAPWCGHCKNLVPEWEKAAKALKGVAKVVAVDATEHAELASKYGVRGYPTIKVFGADKRSPSDYQGGRTAKEIVSGAKKAIGELLGKRMG 432
            VLA+AA+LG      +  LY+     + +  +   F  EVTK DGV  VEFYAPWCGHCKNL PEW++AA ALKGVAKVVA+DA+  + +ASK+GV+G+PTIK+FGA+KRSPSDY G RT+  IV    K +  L+ KR G
Sbjct:    7 VLAAAAVLGLAAVKPAAALYSKRDDVIEVTDAT--FKDEVTKADGVVAVEFYAPWCGHCKNLAPEWKRAAAALKGVAKVVALDASSGSSVASKFGVQGFPTIKIFGANKRSPSDYNGARTSDGIVDAVMKEVRALVAKRTG 145          
BLAST of mRNA_M-pyrifera_M_contig77478.18240.1 vs. uniprot
Match: A0A3P8X1D4_CYNSE (Protein disulfide-isomerase A6 n=2 Tax=Cynoglossus semilaevis TaxID=244447 RepID=A0A3P8X1D4_CYNSE)

HSP 1 Score: 147 bits (371), Expect = 1.810e-39
Identity = 76/137 (55.47%), Postives = 99/137 (72.26%), Query Frame = 1
Query:   64 LYTPGRTAVTLISSKKEFAQEVTKFDGVALVEFYAPWCGHCKNLVPEWEKAAKALKGVAKVVAVDATEHAELASKYGVRGYPTIKVFGADKRSPSDYQGGRTAKEIVSGAKKAIGELLGKRMGG-SGGSKGRKEQGG 471
            LY+P    V L SS   F +EV + D + LVEFYAPWCGHC+NL P+W+KAA ALKG+ KV AVDA +H  L  +YGVRG+PTIK+FGA+K  P +YQGGR+++ IV GA  A+  L+ +R+ G SGGS G++  GG
Sbjct:   20 LYSPSDDVVELTSSN--FNREVIQSDSLWLVEFYAPWCGHCRNLAPDWKKAATALKGIVKVGAVDADQHNSLGGQYGVRGFPTIKIFGANKNKPEEYQGGRSSQAIVDGAMNALRNLVKERLSGKSGGSGGQQSSGG 154          
BLAST of mRNA_M-pyrifera_M_contig77478.18240.1 vs. uniprot
Match: A0A6P7I1I6_9TELE (Protein disulfide-isomerase A6 n=2 Tax=Parambassis ranga TaxID=210632 RepID=A0A6P7I1I6_9TELE)

HSP 1 Score: 146 bits (368), Expect = 5.580e-39
Identity = 72/136 (52.94%), Postives = 96/136 (70.59%), Query Frame = 1
Query:   64 LYTPGRTAVTLISSKKEFAQEVTKFDGVALVEFYAPWCGHCKNLVPEWEKAAKALKGVAKVVAVDATEHAELASKYGVRGYPTIKVFGADKRSPSDYQGGRTAKEIVSGAKKAIGELLGKRMGGSGGSKGRKEQGG 471
            LY+P    V L SS   F +EV + D + LVEFYAPWCGHC+NL P+W+KAA ALKG+ K+ AVDA +H  L S+YG+RG+PTIK+FGA+K  P +YQGGR+++ IV GA  A+  L+ +R+ G  G  G  +Q G
Sbjct:   20 LYSPSDDVVELTSSN--FNREVIQSDSLWLVEFYAPWCGHCQNLAPDWKKAATALKGIVKIGAVDADQHKSLGSQYGIRGFPTIKIFGANKNKPEEYQGGRSSQAIVDGAMNALRTLVKERLSGKSGGSGYNKQSG 153          
BLAST of mRNA_M-pyrifera_M_contig77478.18240.1 vs. uniprot
Match: A0A811LPV5_9BILA (Protein disulfide-isomerase n=1 Tax=Bursaphelenchus okinawaensis TaxID=465554 RepID=A0A811LPV5_9BILA)

HSP 1 Score: 145 bits (366), Expect = 9.890e-39
Identity = 71/113 (62.83%), Postives = 87/113 (76.99%), Query Frame = 1
Query:  115 FAQEVTKFDGVALVEFYAPWCGHCKNLVPEWEKAAKALKGVAKVVAVDATEHAELASKYGVRGYPTIKVFGADKRSPSDYQGGRTAKEIVSGAKKAIGELLGKRMGGSGGSKG 453
            F +EV  +DGV LVEF+APWCGHCKNLVPE++KAAKALKG+AKV AVDAT+   LAS+YGV+GYPTIKVFGADK+ P+D+ G RTA+     A   I ++  KR+GG   S G
Sbjct:   33 FKKEVLDYDGVVLVEFFAPWCGHCKNLVPEYKKAAKALKGLAKVAAVDATQAQGLASQYGVQGYPTIKVFGADKKKPTDHNGARTAQAFTDSALNEIRKIANKRLGGKSTSSG 145          
BLAST of mRNA_M-pyrifera_M_contig77478.18240.1 vs. uniprot
Match: A0A7N8WRS8_9TELE (Protein disulfide-isomerase A6 n=2 Tax=Mastacembelus armatus TaxID=205130 RepID=A0A7N8WRS8_9TELE)

HSP 1 Score: 145 bits (365), Expect = 1.410e-38
Identity = 73/134 (54.48%), Postives = 95/134 (70.90%), Query Frame = 1
Query:   64 LYTPGRTAVTLISSKKEFAQEVTKFDGVALVEFYAPWCGHCKNLVPEWEKAAKALKGVAKVVAVDATEHAELASKYGVRGYPTIKVFGADKRSPSDYQGGRTAKEIVSGAKKAIGELLGKRMGGSGGSKGRKEQ 465
            LY+     V L SS   F +EV + D + LVEFYAPWCGHC+NL P+W+KAA ALKG+ KV AVDA +H  L S+YGVRG+PTIK+FGA+K  P +YQGGR+++ IV GA  A+  L+ +R+ G  GS G  +Q
Sbjct:   35 LYSASDDVVDLTSSN--FNREVIQSDSLWLVEFYAPWCGHCQNLAPDWKKAATALKGIVKVGAVDADQHKSLGSQYGVRGFPTIKIFGANKNKPEEYQGGRSSQAIVDGAMNALRALVKERLSGKSGSSGYNKQ 166          
BLAST of mRNA_M-pyrifera_M_contig77478.18240.1 vs. uniprot
Match: A0A3Q3L5P8_9TELE (Protein disulfide-isomerase A6 n=5 Tax=Percomorphaceae TaxID=1489872 RepID=A0A3Q3L5P8_9TELE)

HSP 1 Score: 145 bits (365), Expect = 1.430e-38
Identity = 73/134 (54.48%), Postives = 95/134 (70.90%), Query Frame = 1
Query:   64 LYTPGRTAVTLISSKKEFAQEVTKFDGVALVEFYAPWCGHCKNLVPEWEKAAKALKGVAKVVAVDATEHAELASKYGVRGYPTIKVFGADKRSPSDYQGGRTAKEIVSGAKKAIGELLGKRMGGSGGSKGRKEQ 465
            LY+     V L SS   F +EV + D + LVEFYAPWCGHC+NL P+W+KAA ALKG+ KV AVDA +H  L S+YGVRG+PTIK+FGA+K  P +YQGGR+++ IV GA  A+  L+ +R+ G  GS G  +Q
Sbjct:   20 LYSASDDVVDLTSSN--FNREVIQSDSLWLVEFYAPWCGHCQNLAPDWKKAATALKGIVKVGAVDADQHKSLGSQYGVRGFPTIKIFGANKNKPEEYQGGRSSQAIVDGAMNALRALVKERLSGKSGSSGYNKQ 151          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig77478.18240.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A4D9CTT8_9STRA3.020e-4256.03Thioredoxin domain-containing protein n=1 Tax=Nann... [more]
A0A8J5XM45_DIALT3.100e-4157.78Uncharacterized protein n=1 Tax=Diacronema lutheri... [more]
A0A6V1Q1R9_HETAK6.920e-4071.72Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
UPI001C08AA9B7.040e-4055.63protein disulfide-isomerase A6 n=2 Tax=Alosa TaxID... [more]
A0A5A8DQB4_CAFRO7.660e-4055.32Uncharacterized protein n=2 Tax=Sar TaxID=2698737 ... [more]
A0A3P8X1D4_CYNSE1.810e-3955.47Protein disulfide-isomerase A6 n=2 Tax=Cynoglossus... [more]
A0A6P7I1I6_9TELE5.580e-3952.94Protein disulfide-isomerase A6 n=2 Tax=Parambassis... [more]
A0A811LPV5_9BILA9.890e-3962.83Protein disulfide-isomerase n=1 Tax=Bursaphelenchu... [more]
A0A7N8WRS8_9TELE1.410e-3854.48Protein disulfide-isomerase A6 n=2 Tax=Mastacembel... [more]
A0A3Q3L5P8_9TELE1.430e-3854.48Protein disulfide-isomerase A6 n=5 Tax=Percomorpha... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig77478contigM-pyrifera_M_contig77478:414..1007 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score150.6
Seed ortholog evalue6.1e-34
Seed eggNOG ortholog244447.XP_008311310.1
Preferred namePDIA6
KEGG koko:K09584
KEGG Pathwayko04141,map04141
Hectar predicted targeting categorysignal peptide
GOsGO:0001775,GO:0003674,GO:0003756,GO:0003824,GO:0005488,GO:0005509,GO:0005575,GO:0005576,GO:0005615,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005788,GO:0005789,GO:0005790,GO:0005793,GO:0005829,GO:0005886,GO:0006457,GO:0006464,GO:0006807,GO:0006810,GO:0006897,GO:0006909,GO:0006950,GO:0006986,GO:0007154,GO:0007155,GO:0007165,GO:0007596,GO:0007599,GO:0008150,GO:0008152,GO:0009611,GO:0009987,GO:0010033,GO:0012505,GO:0015036,GO:0015037,GO:0016020,GO:0016192,GO:0016491,GO:0016667,GO:0016853,GO:0016860,GO:0016864,GO:0019538,GO:0022610,GO:0023052,GO:0030100,GO:0030168,GO:0030968,GO:0031410,GO:0031974,GO:0031982,GO:0031984,GO:0032501,GO:0032879,GO:0032991,GO:0033554,GO:0034109,GO:0034620,GO:0034663,GO:0034976,GO:0035966,GO:0035967,GO:0036211,GO:0036498,GO:0042060,GO:0042175,GO:0042221,GO:0042470,GO:0043167,GO:0043169,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043277,GO:0043412,GO:0043687,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044421,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0045807,GO:0046872,GO:0048518,GO:0048522,GO:0048770,GO:0050764,GO:0050766,GO:0050789,GO:0050794,GO:0050817,GO:0050878,GO:0050896,GO:0051049,GO:0051050,GO:0051128,GO:0051130,GO:0051179,GO:0051234,GO:0051716,GO:0055114,GO:0060627,GO:0065007,GO:0065008,GO:0070013,GO:0070527,GO:0070887,GO:0071310,GO:0071704,GO:0071944,GO:0097708,GO:0098609,GO:0098657,GO:0098827,GO:0140096,GO:1901564,GO:2000425,GO:2000427
EggNOG free text desc.Protein disulfide isomerase-related protein (provisional)
EggNOG OGs38DXF@33154,3BBEW@33208,3CSZI@33213,48155@7711,48Z1C@7742,49UJK@7898,COG0526@1,KOG0191@2759
EC5.3.4.1
COG Functional cat.O
Best tax levelActinopterygii
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03110,ko04131
Exons2
Model size471
Cds size468
Stop0
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig77478.18240.1prot_M-pyrifera_M_contig77478.18240.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig77478 417..1007 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816675.647673-UTR-M-pyrifera_M_contig77478:413..4161622816675.647673-UTR-M-pyrifera_M_contig77478:413..416Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig77478 414..416 +
1692278112.8269067-UTR-M-pyrifera_M_contig77478:413..4161692278112.8269067-UTR-M-pyrifera_M_contig77478:413..416Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig77478 414..416 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816675.6604004-CDS-M-pyrifera_M_contig77478:416..5861622816675.6604004-CDS-M-pyrifera_M_contig77478:416..586Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig77478 417..586 +
1692278112.8380718-CDS-M-pyrifera_M_contig77478:416..5861692278112.8380718-CDS-M-pyrifera_M_contig77478:416..586Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig77478 417..586 +
1622816675.6722674-CDS-M-pyrifera_M_contig77478:709..10071622816675.6722674-CDS-M-pyrifera_M_contig77478:709..1007Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig77478 710..1007 +
1692278112.8472044-CDS-M-pyrifera_M_contig77478:709..10071692278112.8472044-CDS-M-pyrifera_M_contig77478:709..1007Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig77478 710..1007 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig77478.18240.1

>prot_M-pyrifera_M_contig77478.18240.1 ID=prot_M-pyrifera_M_contig77478.18240.1|Name=mRNA_M-pyrifera_M_contig77478.18240.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=156bp
MRSIVVLASAAILGAVGSWGLYTPGRTAVTLISSKKEFAQEVTKFDGVAL
VEFYAPWCGHCKNLVPEWEKAAKALKGVAKVVAVDATEHAELASKYGVRG
YPTIKVFGADKRSPSDYQGGRTAKEIVSGAKKAIGELLGKRMGGSGGSKG
RKEQGG
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mRNA from alignment at M-pyrifera_M_contig77478:414..1007+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig77478.18240.1 ID=mRNA_M-pyrifera_M_contig77478.18240.1|Name=mRNA_M-pyrifera_M_contig77478.18240.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=594bp|location=Sequence derived from alignment at M-pyrifera_M_contig77478:414..1007+ (Macrocystis pyrifera P11B4 male)
GCGATGCGGTCCATTGTGGTCCTTGCTTCTGCCGCCATTTTGGGCGCAGT GGGCAGCTGGGGTCTGTACACCCCTGGTCGCACTGCGGTGACTCTGATTT CGTCGAAGAAGGAGTTTGCGCAGGAAGTGACCAAGTTTGATGGCGTCGCG CTGGTGGAATTCTATGCTCCCTGGTACGTGTCTGCGGGTGTGGCGTAGTG TTGAGTGGGTTGGCGGGTGTTGGGAGTTGGGTGGAGGGAGTGTTGCTTGG CCACGTGTCTGCGTTTTGAGGTTTTTGTGTTTTTTCTCCGAGGCAGGTGC GGGCACTGCAAGAACCTGGTGCCGGAGTGGGAGAAGGCGGCCAAGGCCTT GAAGGGAGTGGCGAAGGTGGTGGCGGTGGACGCGACAGAGCACGCAGAGC TCGCGTCCAAGTATGGGGTGCGTGGATATCCCACGATCAAGGTGTTTGGG GCGGACAAGCGCAGCCCCTCCGACTACCAGGGCGGGCGCACGGCGAAGGA GATCGTGTCTGGCGCCAAAAAAGCGATCGGAGAGCTTCTGGGAAAGCGAA TGGGTGGGAGCGGGGGTAGCAAGGGTCGAAAAGAGCAAGGAGGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig77478:414..1007+

>mRNA_M-pyrifera_M_contig77478.18240.1 ID=mRNA_M-pyrifera_M_contig77478.18240.1|Name=mRNA_M-pyrifera_M_contig77478.18240.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=936bp|location=Sequence derived from alignment at M-pyrifera_M_contig77478:414..1007+ (Macrocystis pyrifera P11B4 male)
ATGCGGTCCATTGTGGTCCTTGCTTCTGCCGCCATTTTGGGCGCAGTGGG
CAGCTGGGGTCTGTACACCCCTGGTCGCACTGCGGTGACTCTGATTTCGT
CGAAGAAGGAGTTTGCGCAGGAAGTGACCAAGTTTGATGGCGTCGCGCTG
GTGGAATTCTATGCTCCCTGATGCGGTCCATTGTGGTCCTTGCTTCTGCC
GCCATTTTGGGCGCAGTGGGCAGCTGGGGTCTGTACACCCCTGGTCGCAC
TGCGGTGACTCTGATTTCGTCGAAGAAGGAGTTTGCGCAGGAAGTGACCA
AGTTTGATGGCGTCGCGCTGGTGGAATTCTATGCTCCCTGGTGCGGGCAC
TGCAAGAACCTGGTGCCGGAGTGGGAGAAGGCGGCCAAGGCCTTGAAGGG
AGTGGCGAAGGTGGTGGCGGTGGACGCGACAGAGCACGCAGAGCTCGCGT
CCAAGTATGGGGTGCGTGGATATCCCACGATCAAGGTGTTTGGGGCGGAC
AAGCGCAGCCCCTCCGACTACCAGGGCGGGCGCACGGCGAAGGAGATCGT
GTCTGGCGCCAAAAAAGCGATCGGAGAGCTTCTGGGAAAGCGAATGGGTG
GGAGCGGGGGTAGCAAGGGTCGAAAAGAGCAAGGAGGAGTGCGGGCACTG
CAAGAACCTGGTGCCGGAGTGGGAGAAGGCGGCCAAGGCCTTGAAGGGAG
TGGCGAAGGTGGTGGCGGTGGACGCGACAGAGCACGCAGAGCTCGCGTCC
AAGTATGGGGTGCGTGGATATCCCACGATCAAGGTGTTTGGGGCGGACAA
GCGCAGCCCCTCCGACTACCAGGGCGGGCGCACGGCGAAGGAGATCGTGT
CTGGCGCCAAAAAAGCGATCGGAGAGCTTCTGGGAAAGCGAATGGGTGGG
AGCGGGGGTAGCAAGGGTCGAAAAGAGCAAGGAGGA
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