mRNA_M-pyrifera_M_contig73307.17388.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig73307.17388.1
Unique NamemRNA_M-pyrifera_M_contig73307.17388.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig73307.17388.1 vs. uniprot
Match: A0A2E7C126_9GAMM (DNA topoisomerase 1 n=9 Tax=Halieaceae TaxID=1706372 RepID=A0A2E7C126_9GAMM)

HSP 1 Score: 721 bits (1862), Expect = 2.000e-254
Identity = 345/367 (94.01%), Postives = 351/367 (95.64%), Query Frame = 1
Query:    1 RGYVRLENKRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEERLDTVAQGQLDWRDLLDSFYADFRQLLETAEEAEPAGMQPNEPTPTDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLRTKHRCKLCNTAMDSYLIDEQRKLHVCGNNPDCPGYEVEKGKFKIKGYDGPTLVCDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRNGEAAPPKMDPVPMPELTCEKVEDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHTSEIDPKYSFLFSAPVADGDGNRTQVRYSRKAAEQYVMTEQDGKPTGWRAVYRNGAWQVEEGKSTSSAKPA 1101
            RGYVRLENKRFYAEKMGDIVTQRLQNSF ELLDYGFTASMEERLDTVAQG+LDWRDLLDSFYADFRQLLE AEEAEP GMQPNEPTPTDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLR KHRC LCN AMDSYLIDEQRKLHVCGNNPDCPGYEVE GKFKIKGYDGPTL CDKCGAEMQLKTGRFGKYFGCTAE CKNTRKLLRNGEAAPPKMDPVPMPEL CEKV+DHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPH +EIDPKYSFLFSAPV D DGNRTQVRYSRKAAEQYVMTE+DGKPTGWRAVYRNG WQ EEGKST++AK A
Sbjct:  518 RGYVRLENKRFYAEKMGDIVTQRLQNSFTELLDYGFTASMEERLDTVAQGELDWRDLLDSFYADFRQLLEKAEEAEPVGMQPNEPTPTDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLRKKHRCTLCNAAMDSYLIDEQRKLHVCGNNPDCPGYEVETGKFKIKGYDGPTLACDKCGAEMQLKTGRFGKYFGCTAEACKNTRKLLRNGEAAPPKMDPVPMPELACEKVDDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHKNEIDPKYSFLFSAPVKDDDGNRTQVRYSRKAAEQYVMTERDGKPTGWRAVYRNGKWQTEEGKSTAAAKGA 884          
BLAST of mRNA_M-pyrifera_M_contig73307.17388.1 vs. uniprot
Match: A0A6C0UBC2_9GAMM (DNA topoisomerase 1 n=1 Tax=Kineobactrum salinum TaxID=2708301 RepID=A0A6C0UBC2_9GAMM)

HSP 1 Score: 696 bits (1795), Expect = 4.250e-244
Identity = 334/366 (91.26%), Postives = 346/366 (94.54%), Query Frame = 1
Query:    1 RGYVRLENKRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEERLDTVAQGQLDWRDLLDSFYADFRQLLETAEEAEPAGMQPNEPTPTDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLRTKHRCKLCNTAMDSYLIDEQRKLHVCGNNPDCPGYEVEKGKFKIKGYDGPTLVCDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRNGEAAPPKMDPVPMPELTCEKVEDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHTSEIDPKYSFLFSAPVADGDGNRTQVRYSRKAAEQYVMTEQDGKPTGWRAVYRNGAWQVEEGKSTSSAKP 1098
            RGYVRLENKRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEE LD VAQGQLDWR LLD+FYADFR LLE AEE EPAGMQPNEPT TDI CSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLR+KHRCKLCN AMDSYLIDEQRKLHVCGNNPDCPG+EVE+GKFKIKGYDGPTL CDKCGA+MQLKTGRFGKYFGCT+E CKNTRKLLR+GEAAPPKMDPVPMPEL C KVEDHYILRDGAAGLFLAASQFPRNRETRAP+VDELLPH SEIDPKYSFLFSAP  D DGNRTQVRYSRKAAEQYVMTE DGKPTGWRAVYR+G W+VEEGK+ +SAKP
Sbjct:  518 RGYVRLENKRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEEHLDEVAQGQLDWRQLLDTFYADFRGLLEKAEEPEPAGMQPNEPTMTDIACSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLRSKHRCKLCNAAMDSYLIDEQRKLHVCGNNPDCPGFEVEQGKFKIKGYDGPTLECDKCGAQMQLKTGRFGKYFGCTSESCKNTRKLLRSGEAAPPKMDPVPMPELACAKVEDHYILRDGAAGLFLAASQFPRNRETRAPYVDELLPHKSEIDPKYSFLFSAPTVDPDGNRTQVRYSRKAAEQYVMTEHDGKPTGWRAVYRHGKWEVEEGKA-ASAKP 882          
BLAST of mRNA_M-pyrifera_M_contig73307.17388.1 vs. uniprot
Match: A0A2N5Y2E2_9GAMM (DNA topoisomerase 1 n=1 Tax=Kineobactrum sediminis TaxID=1905677 RepID=A0A2N5Y2E2_9GAMM)

HSP 1 Score: 689 bits (1777), Expect = 3.870e-241
Identity = 327/361 (90.58%), Postives = 338/361 (93.63%), Query Frame = 1
Query:    1 RGYVRLENKRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEERLDTVAQGQLDWRDLLDSFYADFRQLLETAEEAEPAGMQPNEPTPTDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLRTKHRCKLCNTAMDSYLIDEQRKLHVCGNNPDCPGYEVEKGKFKIKGYDGPTLVCDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRNGEAAPPKMDPVPMPELTCEKVEDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHTSEIDPKYSFLFSAPVADGDGNRTQVRYSRKAAEQYVMTEQDGKPTGWRAVYRNGAWQVEEGKST 1083
            RGYVRLENKRFYAEKMGDIVTQRLQNSF ELLDYGFTA MEERLD VAQG+LDWR LLD FYADFR+LL+TA E EPAGMQPNEPT TDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLR+KHRC LCN AMDSYLIDEQRKLHVCGNNPDCPG+EVE+GKFKIKGYDGP L CDKCGAEMQLKTGRFGKYFGCT+E CKNTRKLLR+GEAAPPKMDPVPMPEL CEKVEDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPH  EIDPKY+FLFSAPV D +GNR QVRYSRK AEQYVMTEQDGKPTGWRA YRNG WQ EEGKS+
Sbjct:  518 RGYVRLENKRFYAEKMGDIVTQRLQNSFTELLDYGFTARMEERLDEVAQGELDWRQLLDMFYADFRELLDTAVEPEPAGMQPNEPTMTDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLRSKHRCALCNAAMDSYLIDEQRKLHVCGNNPDCPGFEVEEGKFKIKGYDGPVLECDKCGAEMQLKTGRFGKYFGCTSETCKNTRKLLRSGEAAPPKMDPVPMPELACEKVEDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHQDEIDPKYTFLFSAPVVDPEGNRCQVRYSRKTAEQYVMTEQDGKPTGWRAFYRNGVWQAEEGKSS 878          
BLAST of mRNA_M-pyrifera_M_contig73307.17388.1 vs. uniprot
Match: A0A2D8IQ76_9GAMM (DNA topoisomerase 1 n=2 Tax=Haliea sp. TaxID=1932666 RepID=A0A2D8IQ76_9GAMM)

HSP 1 Score: 675 bits (1741), Expect = 5.380e-236
Identity = 318/360 (88.33%), Postives = 334/360 (92.78%), Query Frame = 1
Query:    1 RGYVRLENKRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEERLDTVAQGQLDWRDLLDSFYADFRQLLETAEEAEPAGMQPNEPTPTDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLRTKHRCKLCNTAMDSYLIDEQRKLHVCGNNPDCPGYEVEKGKFKIKGYDGPTLVCDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRNGEAAPPKMDPVPMPELTCEKVEDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHTSEIDPKYSFLFSAPVADGDGNRTQVRYSRKAAEQYVMTEQDGKPTGWRAVYRNGAWQVEEGKS 1080
            RGYVRLEN+RFYAEKMGDIVTQRLQNSFAELLDYGFTASMEE LD VAQG+LDW+ LLD FYADFR  LE AEE +P GMQPNEPT TDIPC+KCGRPMQIRTASTGVFLGCSGY+LPPKERCK+TINL PGDE IREDADDEAESRLLR+KHRC+LCN AMDSYLIDEQRKLHVCGNNPDCPGYEVE GKFKIKGYDGPTL CDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRNGE APPKMDPVPMPEL CEKV+DHY+LRDGAAGLFLAASQFPRNRETRAPFVDELLPH  EIDPKY+FLF APV D +GNRTQVRYSRK+AEQYVM+E DGK TGWRAVYRNG WQ EEGK+
Sbjct:  518 RGYVRLENRRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEEHLDDVAQGELDWKQLLDRFYADFRAQLERAEEPQPVGMQPNEPTMTDIPCAKCGRPMQIRTASTGVFLGCSGYSLPPKERCKHTINLIPGDEVIREDADDEAESRLLRSKHRCRLCNAAMDSYLIDEQRKLHVCGNNPDCPGYEVESGKFKIKGYDGPTLECDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRNGEPAPPKMDPVPMPELACEKVDDHYVLRDGAAGLFLAASQFPRNRETRAPFVDELLPHRDEIDPKYTFLFDAPVTDPEGNRTQVRYSRKSAEQYVMSEVDGKATGWRAVYRNGRWQPEEGKA 877          
BLAST of mRNA_M-pyrifera_M_contig73307.17388.1 vs. uniprot
Match: A0A5C8ZZF0_9GAMM (DNA topoisomerase 1 n=4 Tax=Parahaliea TaxID=1857932 RepID=A0A5C8ZZF0_9GAMM)

HSP 1 Score: 660 bits (1703), Expect = 1.820e-230
Identity = 310/365 (84.93%), Postives = 329/365 (90.14%), Query Frame = 1
Query:    1 RGYVRLENKRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEERLDTVAQGQLDWRDLLDSFYADFRQLLETAEEAEPAGMQPNEPTPTDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLRTKHRCKLCNTAMDSYLIDEQRKLHVCGNNPDCPGYEVEKGKFKIKGYDGPTLVCDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRNGEAAPPKMDPVPMPELTCEKVEDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHTSEIDPKYSFLFSAPVADGDGNRTQVRYSRKAAEQYVMTEQDGKPTGWRAVYRNGAWQVEEGKSTSSAK 1095
            RGYVRLENKRFYAEKMG+IVT+RLQ SF ELLDYGFT+SMEERLD VAQG LDWR+LLD FY +F  LLE AEE +P GMQPNEPT TDIPC  CGRPMQIRTASTGVFLGCSGYALPPKERCK T+NL PGDEAIREDADDEAESRLLR+KHRCK+CN AMDSYLIDEQRKLHVCGNNPDC G+EVE+GKFKIKGYDGPTL CDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLR+GE APPKMDPVPMPEL CEKVEDHY+LRDGAAGLFLAASQFPRNRETRAP+VDELLPH  EIDPKYSFLF APV DGDGNR+QVRYSRK  EQYVMTE DGK TGW+A +    WQVEEGK+T + K
Sbjct:  518 RGYVRLENKRFYAEKMGEIVTERLQKSFTELLDYGFTSSMEERLDEVAQGALDWRELLDRFYGEFSDLLEKAEEPQPEGMQPNEPTMTDIPCKACGRPMQIRTASTGVFLGCSGYALPPKERCKQTVNLVPGDEAIREDADDEAESRLLRSKHRCKICNAAMDSYLIDEQRKLHVCGNNPDCSGFEVEQGKFKIKGYDGPTLECDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRSGEPAPPKMDPVPMPELACEKVEDHYVLRDGAAGLFLAASQFPRNRETRAPYVDELLPHRDEIDPKYSFLFDAPVTDGDGNRSQVRYSRKTKEQYVMTEVDGKATGWKAFHNGSRWQVEEGKATKTTK 882          
BLAST of mRNA_M-pyrifera_M_contig73307.17388.1 vs. uniprot
Match: A0A095VPH9_9GAMM (DNA topoisomerase 1 n=2 Tax=Halieaceae TaxID=1706372 RepID=A0A095VPH9_9GAMM)

HSP 1 Score: 646 bits (1667), Expect = 6.700e-225
Identity = 301/367 (82.02%), Postives = 329/367 (89.65%), Query Frame = 1
Query:    1 RGYVRLENKRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEERLDTVAQGQLDWRDLLDSFYADFRQLLETAEEAEPAGMQPNEPTPTDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLRTKHRCKLCNTAMDSYLIDEQRKLHVCGNNPDCPGYEVEKGKFKIKGYDGPTLVCDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRNGEAAPPKMDPVPMPELTCEKVEDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHTSEIDPKYSFLFSAPVADGDGNRTQVRYSRKAAEQYVMTEQDGKPTGWRAVYRNGAWQVEEGKSTSSAKPA 1101
            RGYVRLENKRFYAEKMGDIVT RLQ SFA+LLDY FTA+MEE+LD +A+G +DWR+LLD FYADFR  LE AE+  P GMQPNEPT TDIPC KCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINL PGDEAIREDAD+EAESRLLR+KHRCKLCNTAMDSYLIDE+RKLHVCGNNPDCPG+EVE+G+FKIKGYDGP + CDKCGAEMQLKTGRFGKYFGCTAE CKNTRKLLR+GEAAPPKMDPVPMPEL CEKVEDHY+LRDGAAGLFLAASQFPRNRETRAPFVDELLPH +EIDPKY ++  AP  D  GNR+QVRYSRK+ EQYVMTE DGK TGW+A+YR+G WQ+E   +  + K A
Sbjct:  520 RGYVRLENKRFYAEKMGDIVTHRLQESFADLLDYNFTATMEEQLDEIAEGAMDWRELLDRFYADFRARLERAEQPRPEGMQPNEPTMTDIPCDKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLIPGDEAIREDADEEAESRLLRSKHRCKLCNTAMDSYLIDEKRKLHVCGNNPDCPGFEVEEGRFKIKGYDGPVIECDKCGAEMQLKTGRFGKYFGCTAEGCKNTRKLLRSGEAAPPKMDPVPMPELACEKVEDHYVLRDGAAGLFLAASQFPRNRETRAPFVDELLPHRNEIDPKYGYILEAPAEDDAGNRSQVRYSRKSREQYVMTENDGKATGWKALYRDGRWQIEAASAGGARKAA 886          
BLAST of mRNA_M-pyrifera_M_contig73307.17388.1 vs. uniprot
Match: A0A7W2YI36_9GAMM (DNA topoisomerase 1 n=1 Tax=Sediminihaliea albiluteola TaxID=2758564 RepID=A0A7W2YI36_9GAMM)

HSP 1 Score: 640 bits (1650), Expect = 2.340e-222
Identity = 307/367 (83.65%), Postives = 330/367 (89.92%), Query Frame = 1
Query:    1 RGYVRLENKRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEERLDTVAQGQLDWRDLLDSFYADFRQLLETAEEAEPAGMQPNEPTPTDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLRTKHRCKLCNTAMDSYLIDEQRKLHVCGNNPDCPGYEVEKGKFKIKGYDGPTLVCDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRNGEAAPPKMDPVPMPELTCEKVEDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHTSEIDPKYSFLFSAPVADGDGNRTQVRYSRKAAEQYVMTEQDGKPTGWRAVYRNGAWQVEEGKSTSSAKPA 1101
            RGYVRLENKRFYAEKMGDIVTQRLQ SF++LLDYGFTA+MEERLD VAQG+LDWR LLDSFY +F+QLLE AEE +P GMQPNEPT TDIPCS+CGRPMQIRTASTGVFLGCSGYALPPKERCK+TINLT GDEAI E  D++AESRLLR+K RC LCNTAMDSYL+DE+RKLHVCGNNPDC G+EVE+GKFKIKGYDGP L CDKCGAEMQLKTGRFGKYFGCTAE CKNTRKLLR+GEAAPPKMDPVPMPEL C KV+DHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPH SEIDPKY+FLF+APV D DGNRTQVRYSRK  EQYVMTE DGK TGW+A Y  G WQV     T++AK A
Sbjct:  518 RGYVRLENKRFYAEKMGDIVTQRLQKSFSDLLDYGFTAAMEERLDQVAQGELDWRVLLDSFYEEFQQLLEQAEEPQPLGMQPNEPTMTDIPCSQCGRPMQIRTASTGVFLGCSGYALPPKERCKHTINLTSGDEAIPEGDDEDAESRLLRSKRRCPLCNTAMDSYLMDEKRKLHVCGNNPDCSGFEVEQGKFKIKGYDGPVLECDKCGAEMQLKTGRFGKYFGCTAEGCKNTRKLLRSGEAAPPKMDPVPMPELACVKVDDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHQSEIDPKYAFLFTAPVVDDDGNRTQVRYSRKTKEQYVMTEVDGKATGWKAFYEGGRWQV-----TATAKKA 879          
BLAST of mRNA_M-pyrifera_M_contig73307.17388.1 vs. uniprot
Match: A0A2N5YF41_9GAMM (DNA topoisomerase 1 n=3 Tax=Halioglobus TaxID=1217416 RepID=A0A2N5YF41_9GAMM)

HSP 1 Score: 634 bits (1636), Expect = 1.800e-220
Identity = 299/365 (81.92%), Postives = 319/365 (87.40%), Query Frame = 1
Query:    1 RGYVRLENKRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEERLDTVAQGQLDWRDLLDSFYADFRQLLETAEEAEPAGMQPNEPTPTDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLRTKHRCKLCNTAMDSYLIDEQRKLHVCGNNPDCPGYEVEKGKFKIKGYDGPTLVCDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRNGEAAPPKMDPVPMPELTCEKVEDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHTSEIDPKYSFLFSAPVADGDGNRTQVRYSRKAAEQYVMTEQDGKPTGWRAVYRNGAWQVEEGKSTSSAK 1095
            RGYVRLENKRFYAEKMGDIVTQRLQ SF +LLDYGFTA+MEERLD VA G+ DWRDLLD+FYA+F  LLETA E EP GMQPNEPT TDI C KCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLT GDEAIREDADDEAESR L  +HRC  CN+AMDSYLIDE RKLHVCGNNPDC G+EVE GKFKIKGY+GPTL CDKCG+EMQLK GRFGKYFGCT E+CKNTRKLLRNGE APPKMDPVPMPEL CEKV+DHY+LRDGAAG+FLAAS FPRNRETRAPFVDELLPH   IDPKY+F+FSAP  D DGNR QVRYSRK  EQYVMTE+DGK TGW+A YR G W VE+ KS +  K
Sbjct:  519 RGYVRLENKRFYAEKMGDIVTQRLQRSFTDLLDYGFTANMEERLDDVADGKRDWRDLLDAFYAEFSDLLETANEPEPVGMQPNEPTMTDIACDKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTSGDEAIREDADDEAESRQLLERHRCTKCNSAMDSYLIDEDRKLHVCGNNPDCDGFEVEHGKFKIKGYEGPTLECDKCGSEMQLKNGRFGKYFGCTNEECKNTRKLLRNGEPAPPKMDPVPMPELACEKVDDHYVLRDGAAGMFLAASGFPRNRETRAPFVDELLPHKEAIDPKYTFIFSAPTEDDDGNRAQVRYSRKTKEQYVMTEKDGKATGWKAYYRGGKWDVEQSKSRAKKK 883          
BLAST of mRNA_M-pyrifera_M_contig73307.17388.1 vs. uniprot
Match: UPI00167A3737 (type I DNA topoisomerase n=1 Tax=Halioglobus pacificus TaxID=930806 RepID=UPI00167A3737)

HSP 1 Score: 633 bits (1633), Expect = 5.430e-220
Identity = 299/360 (83.06%), Postives = 320/360 (88.89%), Query Frame = 1
Query:    1 RGYVRLENKRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEERLDTVAQGQLDWRDLLDSFYADFRQLLETAEEAEPAGMQPNEPTPTDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLRTKHRCKLCNTAMDSYLIDEQRKLHVCGNNPDCPGYEVEKGKFKIKGYDGPTLVCDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRNGEAAPPKMDPVPMPELTCEKVEDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHTSEIDPKYSFLFSAPVADGDGNRTQVRYSRKAAEQYVMTEQDGKPTGWRAVYRNGAWQVEEGKS 1080
            RGYVRLENKRFYAEKMGDIVT+RLQ SF++LLDYGFTASMEE LD VA G+LDWR+LLD FY +FR LLE A E  P GMQ NEPT TDI C  C RPMQIRTASTGVFLGCSGYALPPKERCK TINLTPGDEAIREDADDEAESRLL +KHRCKLCNTAMDSYLIDE RKLHVCGNNPDCPG+EVE GKFKIKGYDGP + CDKCG++MQLK GRFGKYFGCT E+CKNTRKLLRNGEAAPPKMDPVPMPEL C+KVEDHY+LRDGAAGLFLAASQFPRNRETRAPFVDE+LPH SEIDPKY+FLFSAP  D DGNRTQ+RYSRK  EQYVM+E DGK TGWRA Y+ G W  E+GK+
Sbjct:  518 RGYVRLENKRFYAEKMGDIVTERLQKSFSDLLDYGFTASMEEHLDEVADGKLDWRNLLDDFYEEFRDLLEKAGEDGPDGMQANEPTMTDIACPTCDRPMQIRTASTGVFLGCSGYALPPKERCKTTINLTPGDEAIREDADDEAESRLLISKHRCKLCNTAMDSYLIDEDRKLHVCGNNPDCPGFEVEHGKFKIKGYDGPVIECDKCGSDMQLKNGRFGKYFGCTNENCKNTRKLLRNGEAAPPKMDPVPMPELVCQKVEDHYVLRDGAAGLFLAASQFPRNRETRAPFVDEILPHKSEIDPKYTFLFSAPATDEDGNRTQIRYSRKTKEQYVMSEVDGKATGWRAHYQGGKWVAEQGKA 877          
BLAST of mRNA_M-pyrifera_M_contig73307.17388.1 vs. uniprot
Match: A0A496XNY9_9GAMM (DNA topoisomerase 1 n=4 Tax=Gammaproteobacteria TaxID=1236 RepID=A0A496XNY9_9GAMM)

HSP 1 Score: 630 bits (1626), Expect = 1.020e-218
Identity = 300/364 (82.42%), Postives = 320/364 (87.91%), Query Frame = 1
Query:    1 RGYVRLENKRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEERLDTVAQGQLDWRDLLDSFYADFRQLLETAEEAEPAGMQPNEPTPTDIPCSKCGRPMQIRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLRTKHRCKLCNTAMDSYLIDEQRKLHVCGNNPDCPGYEVEKGKFKIKGYDGPTLVCDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRNGEAAPPKMDPVPMPELTCEKVEDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHTSEIDPKYSFLFSAPVADGDGNRTQVRYSRKAAEQYVMTEQDGKPTGWRAVYRNGAWQVEEGKSTSSA 1092
            RGYVRLENKRFYAEKMGDIVT RLQ SFAELLDYGFTASMEERLD VAQG+L+W +LLD FYADF  LLE A E EP GMQ NEPT TDI CS CGR MQ+RTASTGVFLGCSGYALPPKERCK+T+NLT GDEAIREDADDEAESRLLRTKH CKLC TAMDSYLIDE RKLHVCG+NP+C G+EVE GKFKIKGY+GP L CDKCGAEMQLK GRFGKYFGCTAEDCKNTRKLLR+GEAAPPKMDPVPMPEL C KVEDHYILRDGAAGLFLAASQFPRNRETRAP V E+LPH  EIDPKY+FLFSAP AD DGN  Q+R+SRK  EQYVMTE+DGK TGW+A Y NG WQ E+ KS +S+
Sbjct:  519 RGYVRLENKRFYAEKMGDIVTHRLQTSFAELLDYGFTASMEERLDDVAQGKLEWHELLDQFYADFSSLLEKAGEEEPVGMQRNEPTMTDIKCSNCGRDMQVRTASTGVFLGCSGYALPPKERCKHTLNLTAGDEAIREDADDEAESRLLRTKHHCKLCGTAMDSYLIDEDRKLHVCGDNPNCRGFEVEHGKFKIKGYEGPVLECDKCGAEMQLKNGRFGKYFGCTAEDCKNTRKLLRSGEAAPPKMDPVPMPELECVKVEDHYILRDGAAGLFLAASQFPRNRETRAPLVSEMLPHQGEIDPKYTFLFSAPTADSDGNPAQIRFSRKTKEQYVMTEKDGKATGWKAFYHNGKWQEEQAKSRASS 882          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig73307.17388.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2E7C126_9GAMM2.000e-25494.01DNA topoisomerase 1 n=9 Tax=Halieaceae TaxID=17063... [more]
A0A6C0UBC2_9GAMM4.250e-24491.26DNA topoisomerase 1 n=1 Tax=Kineobactrum salinum T... [more]
A0A2N5Y2E2_9GAMM3.870e-24190.58DNA topoisomerase 1 n=1 Tax=Kineobactrum sediminis... [more]
A0A2D8IQ76_9GAMM5.380e-23688.33DNA topoisomerase 1 n=2 Tax=Haliea sp. TaxID=19326... [more]
A0A5C8ZZF0_9GAMM1.820e-23084.93DNA topoisomerase 1 n=4 Tax=Parahaliea TaxID=18579... [more]
A0A095VPH9_9GAMM6.700e-22582.02DNA topoisomerase 1 n=2 Tax=Halieaceae TaxID=17063... [more]
A0A7W2YI36_9GAMM2.340e-22283.65DNA topoisomerase 1 n=1 Tax=Sediminihaliea albilut... [more]
A0A2N5YF41_9GAMM1.800e-22081.92DNA topoisomerase 1 n=3 Tax=Halioglobus TaxID=1217... [more]
UPI00167A37375.430e-22083.06type I DNA topoisomerase n=1 Tax=Halioglobus pacif... [more]
A0A496XNY9_9GAMM1.020e-21882.42DNA topoisomerase 1 n=4 Tax=Gammaproteobacteria Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig73307contigM-pyrifera_M_contig73307:2..1102 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score760.4
Seed ortholog evalue3.9e-217
Seed eggNOG ortholog1121937.AUHJ01000005_gene2306
Preferred nametopA
KEGG koko:K03168
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0003916,GO:0003917,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0006139,GO:0006259,GO:0006265,GO:0006725,GO:0006807,GO:0006996,GO:0008150,GO:0008152,GO:0009987,GO:0016043,GO:0016853,GO:0034641,GO:0043170,GO:0044237,GO:0044238,GO:0044260,GO:0044424,GO:0044444,GO:0044464,GO:0046483,GO:0051276,GO:0071103,GO:0071704,GO:0071840,GO:0090304,GO:0140097,GO:1901360
EggNOG free text desc.Releases the supercoiling and torsional tension of DNA, which is introduced during the DNA replication and transcription, by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone
EggNOG OGs1MUFZ@1224,1RNZ2@1236,463YR@72275,COG0550@1,COG0550@2
EC5.99.1.2
COG Functional cat.L
Best tax levelAlteromonadaceae
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03032,ko03400
Exons1
Model size1101
Cds size1101
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816630.3737879-CDS-M-pyrifera_M_contig73307:1..11021622816630.3737879-CDS-M-pyrifera_M_contig73307:1..1102Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig73307 2..1102 +
1692278087.445686-CDS-M-pyrifera_M_contig73307:1..11021692278087.445686-CDS-M-pyrifera_M_contig73307:1..1102Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig73307 2..1102 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig73307.17388.1prot_M-pyrifera_M_contig73307.17388.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig73307 2..1102 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig73307.17388.1

>prot_M-pyrifera_M_contig73307.17388.1 ID=prot_M-pyrifera_M_contig73307.17388.1|Name=mRNA_M-pyrifera_M_contig73307.17388.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=367bp
RGYVRLENKRFYAEKMGDIVTQRLQNSFAELLDYGFTASMEERLDTVAQG
QLDWRDLLDSFYADFRQLLETAEEAEPAGMQPNEPTPTDIPCSKCGRPMQ
IRTASTGVFLGCSGYALPPKERCKNTINLTPGDEAIREDADDEAESRLLR
TKHRCKLCNTAMDSYLIDEQRKLHVCGNNPDCPGYEVEKGKFKIKGYDGP
TLVCDKCGAEMQLKTGRFGKYFGCTAEDCKNTRKLLRNGEAAPPKMDPVP
MPELTCEKVEDHYILRDGAAGLFLAASQFPRNRETRAPFVDELLPHTSEI
DPKYSFLFSAPVADGDGNRTQVRYSRKAAEQYVMTEQDGKPTGWRAVYRN
GAWQVEEGKSTSSAKPA
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mRNA from alignment at M-pyrifera_M_contig73307:2..1102+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig73307.17388.1 ID=mRNA_M-pyrifera_M_contig73307.17388.1|Name=mRNA_M-pyrifera_M_contig73307.17388.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=1101bp|location=Sequence derived from alignment at M-pyrifera_M_contig73307:2..1102+ (Macrocystis pyrifera P11B4 male)
CGCGGTTACGTGCGCCTGGAAAACAAGCGCTTCTACGCGGAAAAGATGGG TGACATCGTTACCCAGCGCCTGCAGAACAGTTTTGCCGAGCTGCTCGACT ACGGCTTCACGGCCAGCATGGAGGAACGCCTGGACACCGTTGCCCAGGGG CAACTCGACTGGCGCGACCTGCTCGACAGCTTCTACGCCGATTTCAGGCA ATTGCTGGAAACGGCGGAAGAAGCCGAACCCGCCGGCATGCAACCGAACG AGCCGACACCGACGGATATTCCCTGCAGCAAATGCGGCAGGCCGATGCAG ATTCGTACCGCGAGTACCGGGGTTTTCCTGGGCTGCTCGGGCTACGCGTT GCCACCGAAGGAACGCTGCAAGAACACCATCAACCTGACTCCGGGTGATG AAGCGATTCGTGAGGACGCCGACGATGAGGCGGAATCCCGCCTGCTGCGA ACCAAGCACCGCTGCAAGCTCTGCAATACCGCCATGGACAGTTACCTGAT CGACGAACAGCGCAAGCTGCATGTGTGCGGTAATAATCCTGACTGCCCGG GCTATGAAGTGGAGAAGGGCAAATTCAAGATAAAGGGCTATGACGGCCCG ACACTGGTGTGTGACAAGTGCGGCGCCGAGATGCAGCTGAAAACCGGGCG TTTCGGCAAGTACTTCGGCTGCACCGCCGAGGACTGCAAGAACACACGCA AGCTGTTGCGCAATGGGGAAGCGGCCCCGCCGAAAATGGACCCGGTGCCC ATGCCGGAGCTCACCTGCGAGAAAGTTGAAGACCACTATATTCTGCGCGA CGGTGCGGCTGGCCTGTTCCTGGCCGCCAGTCAATTCCCGCGCAACCGGG AAACGCGCGCGCCTTTTGTGGACGAGCTCCTGCCGCACACCTCGGAGATT GACCCCAAATACAGCTTCCTTTTTTCGGCGCCCGTCGCCGATGGCGATGG CAACCGTACGCAGGTGCGCTACAGTCGCAAGGCGGCCGAGCAGTACGTGA TGACCGAGCAGGACGGCAAACCCACCGGGTGGCGTGCGGTCTACCGCAAT GGCGCCTGGCAGGTCGAGGAGGGCAAGAGTACGTCGAGCGCCAAACCGGC A
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig73307:2..1102+

>mRNA_M-pyrifera_M_contig73307.17388.1 ID=mRNA_M-pyrifera_M_contig73307.17388.1|Name=mRNA_M-pyrifera_M_contig73307.17388.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=2202bp|location=Sequence derived from alignment at M-pyrifera_M_contig73307:2..1102+ (Macrocystis pyrifera P11B4 male)
CGCGGTTACGTGCGCCTGGAAAACAAGCGCTTCTACGCGGAAAAGATGGG
TGACATCGTTACCCAGCGCCTGCAGAACAGTTTTGCCGAGCTGCTCGACT
ACGGCTTCACGGCCAGCATGGAGGAACGCCTGGACACCGTTGCCCAGGGG
CAACTCGACTGGCGCGACCTGCTCGACAGCTTCTACGCCGATTTCAGGCA
ATTGCTGGAAACGGCGGAAGAAGCCGAACCCGCCGGCATGCAACCGAACG
AGCCGACACCGACGGATATTCCCTGCAGCAAATGCGGCAGGCCGATGCAG
ATTCGTACCGCGAGTACCGGGGTTTTCCTGGGCTGCTCGGGCTACGCGTT
GCCACCGAAGGAACGCTGCAAGAACACCATCAACCTGACTCCGGGTGATG
AAGCGATTCGTGAGGACGCCGACGATGAGGCGGAATCCCGCCTGCTGCGA
ACCAAGCACCGCTGCAAGCTCTGCAATACCGCCATGGACAGTTACCTGAT
CGACGAACAGCGCAAGCTGCATGTGTGCGGTAATAATCCTGACTGCCCGG
GCTATGAAGTGGAGAAGGGCAAATTCAAGATAAAGGGCTATGACGGCCCG
ACACTGGTGTGTGACAAGTGCGGCGCCGAGATGCAGCTGAAAACCGGGCG
TTTCGGCAAGTACTTCGGCTGCACCGCCGAGGACTGCAAGAACACACGCA
AGCTGTTGCGCAATGGGGAAGCGGCCCCGCCGAAAATGGACCCGGTGCCC
ATGCCGGAGCTCACCTGCGAGAAAGTTGAAGACCACTATATTCTGCGCGA
CGGTGCGGCTGGCCTGTTCCTGGCCGCCAGTCAATTCCCGCGCAACCGGG
AAACGCGCGCGCCTTTTGTGGACGAGCTCCTGCCGCACACCTCGGAGATT
GACCCCAAATACAGCTTCCTTTTTTCGGCGCCCGTCGCCGATGGCGATGG
CAACCGTACGCAGGTGCGCTACAGTCGCAAGGCGGCCGAGCAGTACGTGA
TGACCGAGCAGGACGGCAAACCCACCGGGTGGCGTGCGGTCTACCGCAAT
GGCGCCTGGCAGGTCGAGGAGGGCAAGAGTACGTCGAGCGCCAAACCGGC
ACGCGGTTACGTGCGCCTGGAAAACAAGCGCTTCTACGCGGAAAAGATGG
GTGACATCGTTACCCAGCGCCTGCAGAACAGTTTTGCCGAGCTGCTCGAC
TACGGCTTCACGGCCAGCATGGAGGAACGCCTGGACACCGTTGCCCAGGG
GCAACTCGACTGGCGCGACCTGCTCGACAGCTTCTACGCCGATTTCAGGC
AATTGCTGGAAACGGCGGAAGAAGCCGAACCCGCCGGCATGCAACCGAAC
GAGCCGACACCGACGGATATTCCCTGCAGCAAATGCGGCAGGCCGATGCA
GATTCGTACCGCGAGTACCGGGGTTTTCCTGGGCTGCTCGGGCTACGCGT
TGCCACCGAAGGAACGCTGCAAGAACACCATCAACCTGACTCCGGGTGAT
GAAGCGATTCGTGAGGACGCCGACGATGAGGCGGAATCCCGCCTGCTGCG
AACCAAGCACCGCTGCAAGCTCTGCAATACCGCCATGGACAGTTACCTGA
TCGACGAACAGCGCAAGCTGCATGTGTGCGGTAATAATCCTGACTGCCCG
GGCTATGAAGTGGAGAAGGGCAAATTCAAGATAAAGGGCTATGACGGCCC
GACACTGGTGTGTGACAAGTGCGGCGCCGAGATGCAGCTGAAAACCGGGC
GTTTCGGCAAGTACTTCGGCTGCACCGCCGAGGACTGCAAGAACACACGC
AAGCTGTTGCGCAATGGGGAAGCGGCCCCGCCGAAAATGGACCCGGTGCC
CATGCCGGAGCTCACCTGCGAGAAAGTTGAAGACCACTATATTCTGCGCG
ACGGTGCGGCTGGCCTGTTCCTGGCCGCCAGTCAATTCCCGCGCAACCGG
GAAACGCGCGCGCCTTTTGTGGACGAGCTCCTGCCGCACACCTCGGAGAT
TGACCCCAAATACAGCTTCCTTTTTTCGGCGCCCGTCGCCGATGGCGATG
GCAACCGTACGCAGGTGCGCTACAGTCGCAAGGCGGCCGAGCAGTACGTG
ATGACCGAGCAGGACGGCAAACCCACCGGGTGGCGTGCGGTCTACCGCAA
TGGCGCCTGGCAGGTCGAGGAGGGCAAGAGTACGTCGAGCGCCAAACCGG
CA
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