prot_M-pyrifera_M_contig8349.19496.1 (polypeptide) Macrocystis pyrifera P11B4 male

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview
NamemRNA_M-pyrifera_M_contig8349.19496.1
Unique Nameprot_M-pyrifera_M_contig8349.19496.1
Typepolypeptide
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Sequence length277
Homology
BLAST of mRNA_M-pyrifera_M_contig8349.19496.1 vs. uniprot
Match: D8LHV0_ECTSI (Diacylglycerol kinase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LHV0_ECTSI)

HSP 1 Score: 298 bits (764), Expect = 1.900e-93
Identity = 194/293 (66.21%), Postives = 206/293 (70.31%), Query Frame = 0
Query:    1 QERNRRAWAGRTSNHPLSYPQTTQHMTTAWGFDTSGLVDPAGXXXXXXXXXXXXXXXXXX-----------------APATVPLPNEIETLGRLRAGVGGGRRDAPLGSGGAGAXXXXXXXGEVGXXXXXXXXXXXXXXXXXXXXXXXXXEGRWDRPMDSRGSSSVRRESKTSFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQVRTLATLPMQVDGEPWKQPPSEITVKLHNQAAMLRPATMTERAVASVLNDVSSAIHSAEQDLIINERQARTLLSRIRRSARLP 276
            +ERNRR WAGRTSNHPLSYPQ+ Q  TT WGFDT GLV+P G               XXX                 A A      E   L R R  +GGG     +G  G G+         V    XXXXXXXXXXXXXXX       EGRW++P DSRGSSS RRESKTSFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQV++ ATLPMQVDGEPWKQPPSEITVKLHNQAAMLRPAT TERAVASVLNDVSSAIH AE+DL+INERQARTLLSRIRRS RLP
Sbjct:  389 EERNRRTWAGRTSNHPLSYPQSQQQRTTMWGFDTGGLVNPGGSLPSGGAGGEAAAVGXXXXXXXXXXXXXXLPRTPAAAAAAVTAGENTRLRRFRTRLGGGG----IGREGMGSGRPPPLAA-VSVEGXXXXXXXXXXXXXXXGESSDSDEGRWEKPRDSRGSSSKRRESKTSFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQVKSSATLPMQVDGEPWKQPPSEITVKLHNQAAMLRPATTTERAVASVLNDVSSAIHGAEEDLVINERQARTLLSRIRRSTRLP 676          
BLAST of mRNA_M-pyrifera_M_contig8349.19496.1 vs. uniprot
Match: A0A6H5LB27_9PHAE (Diacylglycerol kinase n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5LB27_9PHAE)

HSP 1 Score: 291 bits (744), Expect = 2.010e-86
Identity = 191/299 (63.88%), Postives = 203/299 (67.89%), Query Frame = 0
Query:    1 QERNRRAWAGRTSNHPLSYPQTTQHMTTAWGFDTSGLVDPAGXXXXXXXXXXXXXXXXXXA-----------PATVPLPNEIETLG--------RLRAGVGGGRRDAPLGSGG----AGAXXXXXXXGEVGXXXXXXXXXXXXXXXXXXXXXXXXXEGRWDRPMDSRGSSSVRRESKTSFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQVRTLATLPMQVDGEPWKQPPSEITVKLHNQAAMLRPATMTERAVASVLNDVSSAIHSAEQDLIINERQARTLLSRIRRSARLP 276
            +ERNRR WAGRTSNHPLSYPQ+ Q  TT WGFDT GLV+P G     XXXXXXXXXXXXX            P T        T+G        R R G GG  R+  +GSG     A         GE G                         EGRW++P DSRGSSS RRESKTSFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQV++ ATLPMQVDGEPWKQPPSEITVKL NQAAMLRPAT TERAVASVLNDVSSAIH AE+DL+INERQAR LLSRIRRS RLP
Sbjct: 1127 EERNRRTWAGRTSNHPLSYPQSQQQRTTMWGFDTGGLVNPGGSLSSGXXXXXXXXXXXXXXXXXXXXXXXXMPRTPAXXXXXVTVGENTRLRRFRTRLGGGGVGREG-MGSGRPPPLAAGSVEGGSAGEEGSWGGGSDAGESSDSD----------EGRWEKPRDSRGSSSKRRESKTSFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQVKSSATLPMQVDGEPWKQPPSEITVKLLNQAAMLRPATTTERAVASVLNDVSSAIHGAEEDLVINERQARILLSRIRRSTRLP 1414          
BLAST of mRNA_M-pyrifera_M_contig8349.19496.1 vs. uniprot
Match: I0Z0Q3_COCSC (DAGKa domain-containing protein n=1 Tax=Coccomyxa subellipsoidea (strain C-169) TaxID=574566 RepID=I0Z0Q3_COCSC)

HSP 1 Score: 103 bits (256), Expect = 5.000e-22
Identity = 56/118 (47.46%), Postives = 75/118 (63.56%), Query Frame = 0
Query:  152 ESKTSFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQVRTLATLPMQVDGEPWKQPPSEITVKLHNQAAMLRPATMTERAVASVLNDVSSAIHSAEQDLIINERQARTLLSRI 269
            ++  SF DGVLEV  V G  HLGQ+Q+GLSRA+ +AQCR  ++  L  LPMQ+DGEPW+QPP+ + + L  QA MLR   +    VA+V   VS  + S EQ  +I+  Q  TL + I
Sbjct:  204 DAPQSFCDGVLEVCGVYGSWHLGQLQVGLSRAIRLAQCRSARITALEALPMQIDGEPWRQPPATLDISLKGQAFMLR--RIESGPVAAVARAVSDVLESCEQKGVISAGQRHTLTAEI 319          
BLAST of mRNA_M-pyrifera_M_contig8349.19496.1 vs. uniprot
Match: A0A836CH11_9STRA (Diacylglycerol kinase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CH11_9STRA)

HSP 1 Score: 105 bits (262), Expect = 7.210e-22
Identity = 57/128 (44.53%), Postives = 84/128 (65.62%), Query Frame = 0
Query:  143 SRGSSSVRRESK---TSFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQVRTLATLPMQVDGEPWKQPPSEITVKLHNQAAMLRPATMTERAVASVLNDVSSAIHSAEQDLIINERQARTLLS 267
            S GS+  RR +    +   DG+LEVVAV GVLHLG+IQ+G+     VAQ +E+++RT  TLPMQVDGEPW+Q  S++ V+ H QA+MLRP+T  E  +   +      + SA++  ++++  AR L+S
Sbjct:  718 SGGSAEARRIAAFGPSRIDDGLLEVVAVTGVLHLGRIQVGIGSGYPVAQGKELRIRTTRTLPMQVDGEPWRQAASDMVVRAHPQASMLRPSTPEEGRLRDKITFHEQVVKSAKEKGLVDDELARKLVS 845          
BLAST of mRNA_M-pyrifera_M_contig8349.19496.1 vs. uniprot
Match: A0A8K1CI23_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CI23_PYTOL)

HSP 1 Score: 99.0 bits (245), Expect = 1.200e-19
Identity = 57/130 (43.85%), Postives = 79/130 (60.77%), Query Frame = 0
Query:  142 DSRGSSSVRRESKTSFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQVRTLATLPMQVDGEPWKQPPSEITVKLHNQAAMLRPATMTERAVASVLNDVSSAIHSAEQDLIINERQARTLLSRIRR 271
            D  G +S  R   +S HDG+L+VVAV G LHLGQ+Q+GLS+A+ + Q + + ++   TLPMQ+DGEPW+Q PSE+ +  H QA ML   T+ ER    V   V   +  AE   +I+  Q   LL+ I R
Sbjct:  606 DDGGLASFARVGPSSPHDGLLDVVAVYGTLHLGQMQVGLSKAVRLCQAKHVSIQLKETLPMQIDGEPWQQEPSEVDISFHQQAFMLS-RTVQERDF--VTRRVGEVLDWAENTGVISSTQRDVLLAEIAR 732          
BLAST of mRNA_M-pyrifera_M_contig8349.19496.1 vs. uniprot
Match: A0A8J9S8J6_9CHLO (Probable diacylglycerol kinase gamma at C-terminar half n=1 Tax=Coccomyxa sp. Obi TaxID=2315456 RepID=A0A8J9S8J6_9CHLO)

HSP 1 Score: 99.0 bits (245), Expect = 1.310e-19
Identity = 54/114 (47.37%), Postives = 72/114 (63.16%), Query Frame = 0
Query:  156 SFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQVRTLATLPMQVDGEPWKQPPSEITVKLHNQAAMLRPATMTERAVASVLNDVSSAIHSAEQDLIINERQARTLLSRI 269
            SF DGVLEV  V G  HLGQ+Q+GLSRA+ +AQCR +++  L  LP+Q+DGEPW QPP+ + + L  QA MLR   +    VA+V   VS  + S E   +I+  Q  TL + I
Sbjct:  929 SFCDGVLEVCGVYGSWHLGQLQVGLSRAIRLAQCRSVRITALEALPVQIDGEPWHQPPATLDISLKGQAFMLR--RIESGPVAAVARAVSDVLESCETRNVISAAQRHTLTAEI 1040          
BLAST of mRNA_M-pyrifera_M_contig8349.19496.1 vs. uniprot
Match: A0A0A9P257_ARUDO (Cl798_1 n=4 Tax=Arundo donax TaxID=35708 RepID=A0A0A9P257_ARUDO)

HSP 1 Score: 89.7 bits (221), Expect = 1.020e-18
Identity = 49/114 (42.98%), Postives = 72/114 (63.16%), Query Frame = 0
Query:  156 SFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQVRTLATLPMQVDGEPWKQPPSEITVKLHNQAAMLRPATMTERAVASVLNDVSSAIHSAEQDLIINERQARTLLSRI 269
            S HD  LEVV+  G+LHLG++Q+GLSRA  +AQ   ++++   T+P+QVDGEPW Q P  I V  HNQA ML+   ++E  +    + ++  + +AE   +I+  Q RTLL  I
Sbjct:   21 SMHDKKLEVVSFTGMLHLGRLQVGLSRAQRLAQGHHIKIQISTTMPIQVDGEPWSQEPCTIEVSHHNQAFMLK--RVSEEPLGHAASIMADILENAEDSGVISALQKRTLLQEI 132          
BLAST of mRNA_M-pyrifera_M_contig8349.19496.1 vs. uniprot
Match: A0A7R9VPB5_9CHLO (Hypothetical protein n=1 Tax=Chlamydomonas euryale TaxID=1486919 RepID=A0A7R9VPB5_9CHLO)

HSP 1 Score: 93.2 bits (230), Expect = 1.130e-18
Identity = 55/130 (42.31%), Postives = 80/130 (61.54%), Query Frame = 0
Query:  140 PMDSRGSSSVRRESKTSFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQVRTLATLPMQVDGEPWKQPPSEITVKLHNQAAMLR--PATMTERAVASVLNDVSSAIHSAEQDLIINERQARTLLS 267
            P+     ++V  E + + HDG LEVVA+ GVLH+G++ +GLSRA  +AQ   + V TL TLP+QVDGEPW QPP+++T+   + A +LR  PA       A+V   V  A+  A +   ++ERQ   LL+
Sbjct:  142 PLPGPRGAAVTAERRPALHDGTLEVVAIGGVLHMGRLAVGLSRAQRLAQGSSVTVTTLDTLPVQVDGEPWLQPPAQLTIVHRSMALVLRRMPADSPAARAAAV---VRQALDDAVRAGGLSERQRAELLA 268          
BLAST of mRNA_M-pyrifera_M_contig8349.19496.1 vs. uniprot
Match: A0A024G7F1_9STRA (Diacylglycerol kinase n=1 Tax=Albugo candida TaxID=65357 RepID=A0A024G7F1_9STRA)

HSP 1 Score: 95.9 bits (237), Expect = 1.360e-18
Identity = 58/131 (44.27%), Postives = 83/131 (63.36%), Query Frame = 0
Query:  142 DSRGSSSVRRESKTSF-HDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQVRTLATLPMQVDGEPWKQPPSEITVKLHNQAAMLRPATMTERAVASVLNDVSSAIHSAEQDLIINERQARTLLSRIRR 271
            D   S S R E K+S  +DG+L+VVAV G LHLGQ+Q+GLS+A+ + QCR+++VR   TLP+Q+DGEPW Q    ++V  H+QA ML   + + R   +V + V   ++ AE   II+  Q   LL+ I R
Sbjct:  625 DFSDSKSARCELKSSSPNDGLLDVVAVYGTLHLGQMQVGLSKAVRLCQCRKVEVRVKETLPLQIDGEPWLQSECLLSVSFHDQAHML---SRSVRERDNVTHKVGEVLNWAENTNIISSSQRDILLAEIAR 752          
BLAST of mRNA_M-pyrifera_M_contig8349.19496.1 vs. uniprot
Match: A0A7C9F2E9_OPUST (DAGKa domain-containing protein n=1 Tax=Opuntia streptacantha TaxID=393608 RepID=A0A7C9F2E9_OPUST)

HSP 1 Score: 89.7 bits (221), Expect = 2.370e-18
Identity = 50/112 (44.64%), Postives = 73/112 (65.18%), Query Frame = 0
Query:  156 SFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQVRTLATLPMQVDGEPWKQPPSEITVKLHNQAAMLRPATMTERA-VASVLNDVSSAIHSAEQDLIINERQARTLL 266
            S HD VLEVV+V G  HLG++Q+GLSRA  +AQ ++++++  A+LP+Q+DGEPW Q P  + +  H+QA ML+ A        A++L DV   + +AE   +IN  Q R LL
Sbjct:   53 SMHDKVLEVVSVSGTWHLGKLQVGLSRAQRLAQGQKIKIQLFASLPVQIDGEPWLQQPCTLYISHHSQAFMLKRAAAEPLGHAAAILTDV---LENAESGHVINASQKRALL 161          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig8349.19496.1 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LHV0_ECTSI1.900e-9366.21Diacylglycerol kinase n=1 Tax=Ectocarpus siliculos... [more]
A0A6H5LB27_9PHAE2.010e-8663.88Diacylglycerol kinase n=1 Tax=Ectocarpus sp. CCAP ... [more]
I0Z0Q3_COCSC5.000e-2247.46DAGKa domain-containing protein n=1 Tax=Coccomyxa ... [more]
A0A836CH11_9STRA7.210e-2244.53Diacylglycerol kinase n=1 Tax=Tribonema minus TaxI... [more]
A0A8K1CI23_PYTOL1.200e-1943.85Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A8J9S8J6_9CHLO1.310e-1947.37Probable diacylglycerol kinase gamma at C-terminar... [more]
A0A0A9P257_ARUDO1.020e-1842.98Cl798_1 n=4 Tax=Arundo donax TaxID=35708 RepID=A0A... [more]
A0A7R9VPB5_9CHLO1.130e-1842.31Hypothetical protein n=1 Tax=Chlamydomonas euryale... [more]
A0A024G7F1_9STRA1.360e-1844.27Diacylglycerol kinase n=1 Tax=Albugo candida TaxID... [more]
A0A7C9F2E9_OPUST2.370e-1844.64DAGKa domain-containing protein n=1 Tax=Opuntia st... [more]

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InterPro
Analysis Name: InterProScan on OGS1.0 of Macrocystis pyrifera male
Date Performed: 2022-09-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000756Diacylglycerol kinase, accessory domainSMARTSM00045dagk_c4b_2coord: 63..208
e-value: 3.8E-4
score: -31.7
IPR000756Diacylglycerol kinase, accessory domainPFAMPF00609DAGK_acccoord: 133..208
e-value: 2.0E-15
score: 57.5
IPR037607Diacylglycerol kinasePANTHERPTHR11255DIACYLGLYCEROL KINASEcoord: 152..260
NoneNo IPR availablePANTHERPTHR11255:SF80DIACYLGLYCEROL KINASE ETAcoord: 152..260
IPR016064NAD kinase/diacylglycerol kinase-like domain superfamilySUPERFAMILY111331NAD kinase/diacylglycerol kinase-likecoord: 154..228

Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig8349contigM-pyrifera_M_contig8349:230..4248 +
Analyses
This polypeptide is derived from or has results from the following analyses
Analysis NameDate Performed
InterProScan on OGS1.0 of Macrocystis pyrifera male2022-09-29
Diamond blastp: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-16
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Relationships

This polypeptide derives from the following mRNA feature(s):

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig8349.19496.1mRNA_M-pyrifera_M_contig8349.19496.1Macrocystis pyrifera P11B4 malemRNAM-pyrifera_M_contig8349 230..4248 +


Sequences
The following sequences are available for this feature:

polypeptide sequence

>prot_M-pyrifera_M_contig8349.19496.1 ID=prot_M-pyrifera_M_contig8349.19496.1|Name=mRNA_M-pyrifera_M_contig8349.19496.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=277bp
QERNRRAWAGRTSNHPLSYPQTTQHMTTAWGFDTSGLVDPAGGGGGGRRP
PRGEAVVAAAAPATVPLPNEIETLGRLRAGVGGGRRDAPLGSGGAGAGAG
AGAAGEVGGVASGVNGGEVGGGGAGSRSGSESGEGRWDRPMDSRGSSSVR
RESKTSFHDGVLEVVAVEGVLHLGQIQLGLSRALAVAQCREMQVRTLATL
PMQVDGEPWKQPPSEITVKLHNQAAMLRPATMTERAVASVLNDVSSAIHS
AEQDLIINERQARTLLSRIRRSARLP*
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Annotated Terms
The following terms have been associated with this polypeptide:
Vocabulary: INTERPRO
TermDefinition
IPR000756Diacylglycerol_kin_accessory
IPR037607DGK
IPR016064NAD/diacylglycerol_kinase_sf