prot_M-pyrifera_M_contig77277.18204.1 (polypeptide) Macrocystis pyrifera P11B4 male

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview
NamemRNA_M-pyrifera_M_contig77277.18204.1
Unique Nameprot_M-pyrifera_M_contig77277.18204.1
Typepolypeptide
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Sequence length229
Homology
BLAST of mRNA_M-pyrifera_M_contig77277.18204.1 vs. uniprot
Match: A0A7S4ISD1_9EUKA (Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4ISD1_9EUKA)

HSP 1 Score: 226 bits (577), Expect = 1.710e-71
Identity = 114/227 (50.22%), Postives = 149/227 (65.64%), Query Frame = 0
Query:    1 DAAIKWKALDSNPEVLTTFSRKLGLPEVRPDCMENWAIGDIFGLDEDLLGFVPQPCLAVLLLIPCTTKTPDTGNAEHGQKLFYMKQPDALGNACGTIALFHAFANHMAELGMSD-GPLAAWIDRVKDMTPEQRGDALNSQEDLNDLHNSSAVQGATEVKVEGVEHHFICFTSIEGHLFELDGRKSGPVCHGEIKEASMLHAAARVIKEAYIAPNPDLCEFAMMSLAP 226
            +A  KWKAL+SNPEVLT+  RKLGL          WA  DIFG D DLL  VP PC+AV+LL P   KTP+  +     +LFY+ Q   LGNACGTIA+ H+FAN++  L   D   L  ++ ++K  TP +RG+ LN   ++N LHN  A +G TEV+   V +HF+CFT++ G LFELDG K+GP+ HGEI EA +L +AA  I+E Y+ PNPD  EFAM+ L+P
Sbjct:    9 NARRKWKALESNPEVLTSLGRKLGLKG-------GWAFADIFGFDADLLAMVPSPCVAVILLFPSNKKTPNPTSEVKNDELFYLTQIPELGNACGTIAMLHSFANNIKTLEFDDESVLVNFVKKLKKETPLKRGELLNDDAEINALHNELAHEGQTEVQEGKVNNHFVCFTNVGGRLFELDGTKTGPIDHGEIGEAGLLQSAATTIQEHYVKPNPDCLEFAMIGLSP 228          
BLAST of mRNA_M-pyrifera_M_contig77277.18204.1 vs. uniprot
Match: UPI00188558E0 (ubiquitin carboxyl-terminal hydrolase isozyme L3-like n=1 Tax=Pollicipes pollicipes TaxID=41117 RepID=UPI00188558E0)

HSP 1 Score: 170 bits (430), Expect = 3.290e-49
Identity = 94/237 (39.66%), Postives = 137/237 (57.81%), Query Frame = 0
Query:    4 IKWKALDSNPEVLTTFSRKLGLPEVRPDCMENWAIGDIFGLDEDLLGFVPQPCLAVLLLIPCTTKTPDTGNAE------HGQ----KLFYMKQPDALGNACGTIALFHAFANHMAELGMSDGPLAAWIDRVKDMTPEQRGDALNSQEDLNDLHNSSAVQGATEV--KVEGVEHHFICFTSIEGHLFELDGRKSGPVCHGEIKEASMLHAAARVIKEAYIAPNPDLCEFAMMSLAPAQ 228
            ++W  L+SNP+V+ +F   LGLP         WA+ D++GLD ++L  VPQP  AVLLL P T K       E       GQ    K+++M+Q   +GNACGT+AL HA AN+   L +  GPLA +  R    TPE+R  AL   + L   H ++  QG TE   + + ++ HFI F  ++GHL+ELDGR+  PV HG   + S+L  AA+V+K  ++  +P    F++++LA AQ
Sbjct:   11 VRWLPLESNPDVMNSFLHNLGLPS-------KWAVTDVYGLDPEMLAMVPQPVSAVLLLYPLTEKAVTASADEDAKIQAEGQVQSPKVYFMRQ--TIGNACGTVALMHALANNKEMLQLESGPLAEFFSRTAQQTPEERAAALQLFQQLEAAHEAAGHQGQTEAPSRHDRLDTHFIAFVHVDGHLYELDGRRPAPVNHGPTSQGSLLPDAAKVVKS-FMERDPTEVNFSVLALASAQ 237          
BLAST of mRNA_M-pyrifera_M_contig77277.18204.1 vs. uniprot
Match: A0A1L8ED88_HAEIR (Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Haematobia irritans TaxID=7368 RepID=A0A1L8ED88_HAEIR)

HSP 1 Score: 169 bits (428), Expect = 4.900e-49
Identity = 93/231 (40.26%), Postives = 141/231 (61.04%), Query Frame = 0
Query:    6 WKALDSNPEVLTTFSRKLGLPEVRPDCMENWAIGDIFGLDEDLLGFVPQPCLAVLLLIPCTTKTPD---------TGNAE-HGQKLFYMKQPDALGNACGTIALFHAFANHMAELGMSDGPLAAWIDRVKDMTPEQRGDALNSQEDLNDLHNSSAVQGATEVKVEG-VEHHFICFTSIEGHLFELDGRKSGPVCHGEIKEASMLHAAARVIKEAYIAPNPDLCEFAMMSLA 225
            W  L+SNP+VLT +   LG+ +        W++ D+ GL+ ++L ++P+P  +++LL PC+ K            +G A+ H + LFYM+Q     NACGT+AL H+ AN+ A++ + DGPL  ++D  KD+TPE+RG AL S + + D H + A +G T    E  V HHFI   ++EG L+ELDGRKS PV HG   + + +  AARV KE ++A +P+   F +M+LA
Sbjct:    4 WTPLESNPDVLTKYIHTLGVSK-------EWSLTDVMGLEPEMLEWIPKPAKSIILLFPCSEKYEKHRAEENERLSGEAQNHPKSLFYMRQ--FTSNACGTVALIHSVANN-ADIVLEDGPLKKFLDTAKDLTPEERGKALESDKGVTDSHQTLAQEGQTAANPEEKVNHHFIALVNVEGTLYELDGRKSFPVKHGATTDENFVQDAARVCKE-FMARDPEELRFTVMALA 223          
BLAST of mRNA_M-pyrifera_M_contig77277.18204.1 vs. uniprot
Match: UPI001CA820E8 (ubiquitin carboxyl-terminal hydrolase-like n=1 Tax=Leptopilina heterotoma TaxID=63436 RepID=UPI001CA820E8)

HSP 1 Score: 169 bits (428), Expect = 5.350e-49
Identity = 93/232 (40.09%), Postives = 134/232 (57.76%), Query Frame = 0
Query:    6 WKALDSNPEVLTTFSRKLGLPEVRPDCMENWAIGDIFGLDEDLLGFVPQPCLAVLLLIPCTTKTPDTGNAEHGQK---------LFYMKQPDALGNACGTIALFHAFANHMAELGMSDGPLAAWIDRVKDMTPEQRGDALNSQEDLNDLHNSSAVQGATEVKVEG--VEHHFICFTSIEGHLFELDGRKSGPVCHGEIKEASMLHAAARVIKEAYIAPNPDLCEFAMMSLAP 226
            W  L+SNPEV+T    KLG+P         W I D++GL+ DLL  + +P LA++LL P + +  D G  E  +          ++++KQ   + NACGTIAL H+ AN+  +L + DG L ++I+  KD++P +RGD L    D+ D H   A +G TE   E   V HHF+ F   +GH++ELDGR+ GP+ HG    ++ L  AARV KE Y+  NPD   F M++L P
Sbjct:    8 WVPLESNPEVMTKLCHKLGVPT-------KWNIVDVYGLEPDLLAILTKPVLAMILLYPLSDQEKDRGLKEDSETRESSTVSPDVYFLKQ--FISNACGTIALIHSLANNAEKLELEDGFLKSFIEETKDLSPFERGDRLMQAGDIADTHKELAQEGQTEAPNENARVYHHFVAFIEKDGHIYELDGRRDGPINHGVSSPSTFLEDAARVCKE-YMQRNPDEMGFTMVALTP 229          
BLAST of mRNA_M-pyrifera_M_contig77277.18204.1 vs. uniprot
Match: A0A6J0BIZ4_NEOLC (Ubiquitin carboxyl-terminal hydrolase n=3 Tax=Diprioninae TaxID=410274 RepID=A0A6J0BIZ4_NEOLC)

HSP 1 Score: 169 bits (427), Expect = 6.930e-49
Identity = 97/232 (41.81%), Postives = 136/232 (58.62%), Query Frame = 0
Query:    6 WKALDSNPEVLTTFSRKLGLPEVRPDCMENWAIGDIFGLDEDLLGFVPQPCLAVLLLIPCTTKTPDTGN------AEHGQKL----FYMKQPDALGNACGTIALFHAFANHMAELGMSDGPLAAWIDRVKDMTPEQRGDALNSQEDLNDLHNSSAVQGATEVKVEG--VEHHFICFTSIEGHLFELDGRKSGPVCHGEIKEASMLHAAARVIKEAYIAPNPDLCEFAMMSLA 225
            W  L+SNPEV+T F  KLG+P+       NW I D++G+D DLL  +P+P LA++LL P T K+    N       E GQK+    +YMKQ   + NACGT+AL H  AN+  ++ + DG L +++D  K ++P +RG+ L   E + D H   A +G TE   +   V HHFI F   +G L+ELDGR+S PV HG     + L  A RV +E Y+A +PD   F M++LA
Sbjct:    3 WVPLESNPEVMTKFLHKLGVPK-------NWGIVDVYGVDPDLLAVIPRPVLALILLYPITDKSDRFKNDREAKLKEDGQKISEDIYYMKQ--CVSNACGTVALIHGVANNSDKIQLEDGQLKSFLDSTKGLSPLERGELLAKSEAIIDTHKELAQEGQTEAPGDDSPVNHHFITFVEKDGSLYELDGRQSIPVNHGASTADTFLDDAVRVCRE-YMARDPDEVRFTMVALA 224          
BLAST of mRNA_M-pyrifera_M_contig77277.18204.1 vs. uniprot
Match: UPI001CAA36FA (ubiquitin carboxyl-terminal hydrolase-like n=1 Tax=Leptopilina heterotoma TaxID=63436 RepID=UPI001CAA36FA)

HSP 1 Score: 168 bits (425), Expect = 1.390e-48
Identity = 93/233 (39.91%), Postives = 139/233 (59.66%), Query Frame = 0
Query:    4 IKWKALDSNPEVLTTFSRKLGLPEVRPDCMENWAIGDIFGLDEDLLGFVPQPCLAVLLLIPCT-------TKTPD--TGNAEHGQKLFYMKQPDALGNACGTIALFHAFANHMAELGMSDGPLAAWIDRVKDMTPEQRGDALNSQEDLNDLHNSSAVQGATEVKVEGVEHHFICFTSIEGHLFELDGRKSGPVCHGEIKEASMLHAAARVIKEAYIAPNPDLCEFAMMSLAPA 227
            + W  L+SNPEV+T    KLG+P         W I D++GL+ DLL  + +P LA++LL P +       TK  +   G+A     ++Y+KQ   + NACGTIAL H+ AN++ +L + DG L ++++  K+++P +RG+ L S  D+ D H   A +G TEV  E   HHF+ F   +GH++ELDGR+ GP+ HG    ++ L  AARV KE Y+  NPD   F+++ L PA
Sbjct:    6 MSWVPLESNPEVMTKLFHKLGVPT-------KWNIVDVYGLEPDLLAILTKPVLAMILLYPLSDTEKNRVTKEDNETNGSATISPNVYYLKQ--FISNACGTIALIHSLANNVDKLDLQDGMLKSFLEETKNLSPTERGERLMSAGDIADTHKEFAREGQTEVPDESY-HHFVTFVEKDGHIYELDGRRDGPINHGVSSPSTFLEDAARVCKE-YMKRNPDEMGFSVLVLTPA 227          
BLAST of mRNA_M-pyrifera_M_contig77277.18204.1 vs. uniprot
Match: A0A0L0D141_THETB (Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Thecamonas trahens ATCC 50062 TaxID=461836 RepID=A0A0L0D141_THETB)

HSP 1 Score: 167 bits (422), Expect = 6.080e-48
Identity = 101/239 (42.26%), Postives = 134/239 (56.07%), Query Frame = 0
Query:    1 DAAIKWKALDSNPEVLTTFSRKLGLPEVRPDCMENWAIGDIFGLDEDLLGFVPQPCLAVLLLIPCTTKTPDTGNAEH--------GQKLFYMKQPDALGNACGTIALFHAFANHMAELGMSDGP-LAAWIDRVKDMTPEQRGDALNSQEDLNDLHNSSAVQG--ATEVKVEGVEHHFICFTSIEGHLFELDGRKSGPVCHGEIKEASMLHAAARVIKEAYIAPNPDLCEFAMM--SLAP 226
            DA  +WKA++SNPEV T F+  LG+PE +  CM      DI+GLD+D+L F+PQP +AVLL  P      D  + E             FYM Q D LGNACGTIA+ HA AN ++ L ++D   L  ++D V + TP  RG+   + E +  LHN +A QG  A       +  HFICF   +G L+ELDGRK GP+         +  AAA  IK  ++APNPD   F+M+  SL P
Sbjct:    8 DARDRWKAVESNPEVFTNFAANLGVPEGK--CM----FSDIWGLDDDMLAFLPQPVMAVLLCRPSGLNKVDRPDPESVYGDDGAPADPPFYMTQIDELGNACGTIAMVHALANTVSALDLADDSILRGFLDSVAEATPLVRGETFAANERIRSLHNGTASQGQSAQPESEASIAAHFICFAFHDGILYELDGRKPGPIPLARCSADGVAAAAAAAIKTYFMAPNPDEYNFSMVAYSLVP 240          
BLAST of mRNA_M-pyrifera_M_contig77277.18204.1 vs. uniprot
Match: A0A507EAE3_9FUNG (Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Powellomyces hirtus TaxID=109895 RepID=A0A507EAE3_9FUNG)

HSP 1 Score: 165 bits (418), Expect = 1.820e-47
Identity = 87/235 (37.02%), Postives = 144/235 (61.28%), Query Frame = 0
Query:    6 WKALDSNPEVLTTFSRKLGLPEVRPDCMENWAIGDIFGLDEDLLGFVPQPCLAVLLLIPCTTKTPDTGNAEHGQ----------KLFYMKQPDALGNACGTIALFHAFANHMAELGMSDGPLAAWIDRVKDMTPEQRGDALNSQEDLNDLHNSSAVQGATEV--KVEGVEHHFICFTSIEGHLFELDGRKSGPVCHGEIKEASMLHAAARVIKEAYIAPNPDLCEFAMMSLAPAQ 228
            W  L++NPEV+  ++++LG+         +W+  D++GL++DLL F+P+P  AV+LL P T K  +   AE  +          KL++++Q   + NACGTI L HA AN+  EL + DGPL + +D+  +++PE+R +AL + + L  +H  S+ +G T    +   V+ HF+CF   EGH++E+DGRK  P+ HG  + + +L  + RVIKE ++A +P+   F +++LAP Q
Sbjct:    9 WIPLEANPEVMNKYTQRLGVAS-------SWSFTDVWGLEDDLLAFIPRPVKAVVLLFPVTAKYEEFRRAEEAEIIEDGQVVSPKLYFVQQ--TIPNACGTIGLLHALANNAEELKLEDGPLKSILDQTANISPEERAEALETSKALAQVHEESSQEGQTAAPPRDSDVDLHFVCFVQKEGHVYEMDGRKPFPINHG--RSSDLLMDSVRVIKE-FMARDPENLNFTVIALAPTQ 231          
BLAST of mRNA_M-pyrifera_M_contig77277.18204.1 vs. uniprot
Match: A0A6P3XEG8_DINQU (Ubiquitin carboxyl-terminal hydrolase n=3 Tax=Ponerini TaxID=141711 RepID=A0A6P3XEG8_DINQU)

HSP 1 Score: 164 bits (415), Expect = 4.190e-47
Identity = 93/233 (39.91%), Postives = 136/233 (58.37%), Query Frame = 0
Query:    6 WKALDSNPEVLTTFSRKLGLPEVRPDCMENWAIGDIFGLDEDLLGFVPQPCLAVLLLIPCTTKTPDTGNAEHGQK--------LFYMKQPDALGNACGTIALFHAFANHMAELGMSDGPLAAWIDRVKDMTPEQRGDALNSQEDLNDLHNSSAVQGATEVKVEGVE--HHFICFTSIEGHLFELDGRKSGPVCHGEIKEASMLHAAARVIKEAYIAPNPDLCEFAMMSLAPAQ 228
            W  L+SNPEV+T F  KLG+P+        W+I D++GLD +LL  +P+P L+V+LL P T K   T   E   K        +++MKQ   + NACGTIAL H+ AN++  L + DG L  ++D  K +   +RG+ L + + + D H  SA +G TE   E +E  HHFI F   +G L+ELDGRK  P+ HG     ++L  AARV K+ Y+A +P+   F +++LA  +
Sbjct:    3 WVPLESNPEVMTKFLHKLGVPK-------KWSIIDVYGLDPELLAIIPKPVLSVILLYPVTIKNEKTEEEEESAKDKSDTPDIVYHMKQ--YVSNACGTIALVHSVANNLDVLQLEDGFLKTFLDETKGLPSSERGERLINAQGIIDTHMESAQEGQTEAPGEDMEVYHHFIAFVHKDGSLYELDGRKPIPINHGSTSLETLLEDAARVCKD-YMARDPEEVRFTVVALAATE 225          
BLAST of mRNA_M-pyrifera_M_contig77277.18204.1 vs. uniprot
Match: A0A158NQ48_ATTCE (Ubiquitin carboxyl-terminal hydrolase n=17 Tax=Attini TaxID=143999 RepID=A0A158NQ48_ATTCE)

HSP 1 Score: 164 bits (414), Expect = 6.100e-47
Identity = 88/231 (38.10%), Postives = 138/231 (59.74%), Query Frame = 0
Query:    6 WKALDSNPEVLTTFSRKLGLPEVRPDCMENWAIGDIFGLDEDLLGFVPQPCLAVLLLIPCTTKTPDT---------GNAEHGQKLFYMKQPDALGNACGTIALFHAFANHMAELGMSDGPLAAWIDRVKDMTPEQRGDALNSQEDLNDLHNSSAVQGATEVKVEGVE--HHFICFTSIEGHLFELDGRKSGPVCHGEIKEASMLHAAARVIKEAYIAPNPDLCEFAMMSLA 225
            W  L+SNPEV+T F  KLG+P+        W+I D++GL+ +LL  +P+P LAV+LL P ++K   T           ++  + ++++KQ  ++ NACGTIAL H+ AN++  + + DG    +++  K ++  +RG+ L   +D+ D H  SA +G TE   E VE  HHF+ F   +G L+ELDGRKS P+ HG     ++L  AARV KE Y+  +P+   F +++LA
Sbjct:    3 WVPLESNPEVMTKFLHKLGVPK-------KWSILDVYGLESELLAIIPRPILAVILLYPISSKIEKTKEEQAEAKDNKSDAAESVYHIKQ--SISNACGTIALLHSVANNLDVIQLEDGFFKKFLEETKGLSYIERGERLEKAQDIIDTHMESAQEGQTEAPAEDVEVYHHFVAFVHKDGSLYELDGRKSAPINHGSTTPETLLDDAARVCKE-YMEHDPNEVRFTVVALA 223          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig77277.18204.1 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S4ISD1_9EUKA1.710e-7150.22Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Vann... [more]
UPI00188558E03.290e-4939.66ubiquitin carboxyl-terminal hydrolase isozyme L3-l... [more]
A0A1L8ED88_HAEIR4.900e-4940.26Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Haem... [more]
UPI001CA820E85.350e-4940.09ubiquitin carboxyl-terminal hydrolase-like n=1 Tax... [more]
A0A6J0BIZ4_NEOLC6.930e-4941.81Ubiquitin carboxyl-terminal hydrolase n=3 Tax=Dipr... [more]
UPI001CAA36FA1.390e-4839.91ubiquitin carboxyl-terminal hydrolase-like n=1 Tax... [more]
A0A0L0D141_THETB6.080e-4842.26Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Thec... [more]
A0A507EAE3_9FUNG1.820e-4737.02Ubiquitin carboxyl-terminal hydrolase n=1 Tax=Powe... [more]
A0A6P3XEG8_DINQU4.190e-4739.91Ubiquitin carboxyl-terminal hydrolase n=3 Tax=Pone... [more]
A0A158NQ48_ATTCE6.100e-4738.10Ubiquitin carboxyl-terminal hydrolase n=17 Tax=Att... [more]

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InterPro
Analysis Name: InterProScan on OGS1.0 of Macrocystis pyrifera male
Date Performed: 2022-09-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001578Peptidase C12, ubiquitin carboxyl-terminal hydrolasePRINTSPR00707UBCTHYDRLASEcoord: 88..105
score: 56.35
coord: 176..186
score: 67.53
coord: 160..171
score: 38.1
coord: 8..25
score: 46.03
IPR001578Peptidase C12, ubiquitin carboxyl-terminal hydrolasePFAMPF01088Peptidase_C12coord: 6..207
e-value: 4.9E-47
score: 160.4
IPR001578Peptidase C12, ubiquitin carboxyl-terminal hydrolasePANTHERPTHR10589UBIQUITIN CARBOXYL-TERMINAL HYDROLASEcoord: 5..227
IPR036959Peptidase C12, ubiquitin carboxyl-terminal hydrolase superfamilyGENE3D3.40.532.10coord: 1..227
e-value: 7.4E-60
score: 204.1
NoneNo IPR availablePANTHERPTHR10589:SF17UBIQUITIN CARBOXYL-TERMINAL HYDROLASEcoord: 5..227
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 5..226

Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig77277contigM-pyrifera_M_contig77277:127..1011 -
Analyses
This polypeptide is derived from or has results from the following analyses
Analysis NameDate Performed
InterProScan on OGS1.0 of Macrocystis pyrifera male2022-09-29
Diamond blastp: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-16
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Relationships

This polypeptide derives from the following mRNA feature(s):

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig77277.18204.1mRNA_M-pyrifera_M_contig77277.18204.1Macrocystis pyrifera P11B4 malemRNAM-pyrifera_M_contig77277 127..1011 -


Sequences
The following sequences are available for this feature:

polypeptide sequence

>prot_M-pyrifera_M_contig77277.18204.1 ID=prot_M-pyrifera_M_contig77277.18204.1|Name=mRNA_M-pyrifera_M_contig77277.18204.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=229bp
DAAIKWKALDSNPEVLTTFSRKLGLPEVRPDCMENWAIGDIFGLDEDLLG
FVPQPCLAVLLLIPCTTKTPDTGNAEHGQKLFYMKQPDALGNACGTIALF
HAFANHMAELGMSDGPLAAWIDRVKDMTPEQRGDALNSQEDLNDLHNSSA
VQGATEVKVEGVEHHFICFTSIEGHLFELDGRKSGPVCHGEIKEASMLHA
AARVIKEAYIAPNPDLCEFAMMSLAPAQ*
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Annotated Terms
The following terms have been associated with this polypeptide:
Vocabulary: INTERPRO
TermDefinition
IPR001578Peptidase_C12_UCH
IPR036959Peptidase_C12_UCH_sf
IPR038765Papain-like_cys_pep_sf