prot_M-pyrifera_M_contig1072.1542.1 (polypeptide) Macrocystis pyrifera P11B4 male

BLAST of mRNA_M-pyrifera_M_contig1072.1542.1 vs. uniprot
Match: D7FII3_ECTSI (Methyltransf_11 domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FII3_ECTSI)

HSP 1 Score: 567 bits (1462), Expect = 8.260e-195
Identity = 329/590 (55.76%), Postives = 386/590 (65.42%), Query Frame = 0

Query:    1 VTAVTVDFFRKGSYSSALPATAPLLDSWHQRKPKLQPLRDAAIASSQEESSGREAASMGGLEKSAWAGGEGSDTEPGVCVLWLQADGEVVVGSAQELAKRSKGPTHASAPSAGKPIEPISGE-------------AEGDFPGMEKLSVPRGSVMVWDNAQAEGAQVAVPTAAWDGMVVSFQTLTPEASARILRNRSEREDAKAKAASIRRAAKEAKEAKD-KGPIPGTPVVVSGWRGGVDTTNNTDENADCTTNTMKKSSGSDSXXXXXXSSGRLGFDEDTTDDGKTPLIEKEHVQAVYDTIAPHWSHTRYKPWPRVEEFLRELEAFSLVADAGCGNGKYLGCDAVGGWMLGSDASVNLLQVCKEKFPNAEVLASDCMRLPYRSGVFDAVISIAVLHHFSSEARRLRALSELCRLLRPGGLMLVYAWAIEQEQDSRRSFAAQDVLVPWHLGDQHNHQKKTDLPRNGASANNASSNQQTCQTDXXXXXXXXXXXXTRGGDTSASNRGTWGQSSTNAFGVVGSNSAPTPYAPRHGVRIHAGAGPGGSVVYQRYCHMYSEGELEGLVESVDGLRVVKSYYDRSNWCVVAERKV 576
            VTA TVDFFRKGSYS AL ATAP+        P   P + A  A +  + S  ++A           G   SDTE  VC++WLQA GEVVVGSA +LA  SKGP    A     P   ++GE              EG FPGME++  P+GSVM+W++AQA+G QVAVP+A+ D ++VSFQTLTPEAS+R++ NR ER++AK K+   R+A          KGPIPGTP+VV+ W            N D    T ++    D        + R G      DDG+TP IEKEHVQAVYDTIAPHWSHTRYKPWPRVE+FL ELE  S VADAGCGNGKYLGCD  GG+++GSD SVNLL VCKE+ P AEVLASDCMRLPYRSGVFDA ISIAVLHHFSSE RRLRAL+ELCRL+RPGG +LVYAWAIEQEQDSRRSFAAQDVLVPW L D HN  +  D   +G S N     Q++ +               +G +         G S ++A GVV    APTP APRHGVR+   AG  GSVVYQRYCH+Y+ GELEGLVE VDGLR+V+SYYDRSNWCVVAER V
Sbjct:   38 VTAATVDFFRKGSYSPALAATAPVFVP-----PSSTPPKSARAAGTTGDKSCHDSA----------LGESDSDTEAAVCMIWLQAGGEVVVGSAHQLANCSKGPQEKRAKDTQIPPIEMAGEKKEGARAEVERERGEGLFPGMERVLAPQGSVMMWNSAQAQGLQVAVPSASRDAILVSFQTLTPEASSRVIANRRERKEAKTKSKMFRKAXXXXXXXXXXKGPIPGTPIVVADWE--------EQGNDDGDRGTRRRPEKDDMNSSCNADAERGG-----EDDGRTPSIEKEHVQAVYDTIAPHWSHTRYKPWPRVEKFLSELEPCSFVADAGCGNGKYLGCDTAGGFLVGSDTSVNLLHVCKERLPKAEVLASDCMRLPYRSGVFDAAISIAVLHHFSSEVRRLRALTELCRLVRPGGRLLVYAWAIEQEQDSRRSFAAQDVLVPWQLADHHNRSQGRDTGDDGNSGNTMDGGQESEEPATANVERSGASAPAKGAEDGVV---AAGDSKSHAGGVV----APTPTAPRHGVRVDGVAGTAGSVVYQRYCHVYARGELEGLVERVDGLRLVESYYDRSNWCVVAERDV 592          

BLAST of mRNA_M-pyrifera_M_contig1072.1542.1 vs. uniprot
Match: A0A835YPN8_9STRA (S-adenosyl-L-methionine-dependent methyltransferase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YPN8_9STRA)

HSP 1 Score: 239 bits (609), Expect = 2.630e-71
Identity = 138/308 (44.81%), Postives = 169/308 (54.87%), Query Frame = 0

Query:  272 KTPLIEKEHVQAVYDTIAPHWSHTRYKPWPRVEEFLR-ELEAFSLVADAGCGNGKYLGCDAVGGWMLGSDASVNLLQVCKEKFPN--AEVLASDCMRLPYRSGVFDAVISIAVLHHFSSEARRLRALSELCRLLRPGGLMLVYAWAIEQEQDSRRSFAAQDVLVPWHLGDQHNHQKKTDLPRNGASANNASSNQQTCQTDXXXXXXXXXXXXTRGGDTSASNRGTWGQSSTNAFGVVGSNSAPTPYAPRHGVRIHAGAGPGGSVVYQRYCHMYSEGELEGLVESVDGLRVVKSYYDRSNWCVVAERKV 576
            +TP IE EHV+AVYDTIA HWS TRYKPWPRV E+L    ++  L+AD GCGNGKYLGC   G  ++G DAS  LL +C +      AE+  +D + LPYR G FDA ISIAVLHH S+  RRL A +E+ R+LRPGG +LVYAWA EQ ++S RSF+ QD+LVPWH  D         + R  A A                                          ST   G      A T   P+HG R         S V+QRYCH++ EGE+E L   V G  VV+SYYD SNWC V ++ V
Sbjct:    8 RTPAIEAEHVRAVYDTIATHWSSTRYKPWPRVAEYLHARCKSGHLIADLGCGNGKYLGCAPQGVVVVGCDASAALLSLCSKTPAGRAAELCTADVVLLPYRDGQFDAAISIAVLHHLSTRQRRLAAAAEMVRVLRPGGTVLVYAWAQEQGEESSRSFSHQDMLVPWHFADS--------MRRKSADA------------------------------------------STERHG-----GAKTSQQPQHGYRAEGKH----STVFQRYCHLFKEGEVEALFHRVGGCTVVQSYYDCSNWCAVVQKDV 256          

BLAST of mRNA_M-pyrifera_M_contig1072.1542.1 vs. uniprot
Match: K3W7F8_GLOUD (Methyltransf_11 domain-containing protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3W7F8_GLOUD)

HSP 1 Score: 208 bits (529), Expect = 8.630e-60
Identity = 122/311 (39.23%), Postives = 159/311 (51.13%), Query Frame = 0

Query:  264 DEDTTDDGKTPLIEKEHVQAVYDTIAPHWSHTRYKPWPRVEEFLRELEAFSLVADAGCGNGKYLGCDAVGGWMLGSDASVNLLQVCKEKFPNAEVLASDCMRLPYRSGVFDAVISIAVLHHFSSEARRLRALSELCRLLRPGGLMLVYAWAIEQEQDSRRSFAAQDVLVPWHLGDQHNHQKKTDLPRNGASANNASSNQQTCQTDXXXXXXXXXXXXTRGGDTSASNRGTWGQSSTNAFGVVGSNSAPTPYAPRHGVRIHAGAGPGGSVVYQRYCHMYSEGELEGLVESVDGLRVVKSYYDRSNWCVVAER 574
            DE  T+    P +E EHV +VYD+IAPH+SHTR+ PWP V  FL ++   +LVAD GCGNGKYLG  +    M+GSD S+ LLQ C +   N  +   D +++P R+G FDA ISIAVLHH S+ ARR+  +SEL R+++ GG + + AWA EQ++ S+R F  QDV+V W L      Q K       A A   +  +                               W                                     VVYQRYCH+Y+EGELE LV  V GL+VV++ Y RSNWCV  ER
Sbjct:    6 DEPLTESLAPPALESEHVHSVYDSIAPHFSHTRHHPWPLVTAFLADVAPSALVADVGCGNGKYLG-GSPHVCMIGSDRSLPLLQTCAQSGKNYNLFGCDALKVPLRTGAFDAAISIAVLHHISTRARRIALISELARIVKVGGRVFIVAWAFEQDERSKRQFDEQDVMVEWKL------QNKYVAEPLAAHAKQDADKK-------------------------------W-------------------------------------VVYQRYCHVYTEGELEELVACVPGLKVVETQYSRSNWCVTVER 241          

BLAST of mRNA_M-pyrifera_M_contig1072.1542.1 vs. uniprot
Match: A0A507DN10_9FUNG (Methyltransf_11 domain-containing protein n=2 Tax=Chytriomyces confervae TaxID=246404 RepID=A0A507DN10_9FUNG)

HSP 1 Score: 207 bits (528), Expect = 6.810e-59
Identity = 125/306 (40.85%), Postives = 170/306 (55.56%), Query Frame = 0

Query:  276 IEKEHVQAVYDTIAPHWSHTRYKPWPRVEEFLRELEAFSLVADAGCGNGKYLGCDAVGGWMLGSDASVNLLQVCKEKFPNAEVLASDCMRLPYRSGVFDAVISIAVLHHFSSEARRLRALSELCRLLRPGGLMLVYAWAIEQEQDSRRSFAAQDVLVPWHLGDQHNHQKKTDLPRNGASANNASSNQQTCQTDXXXXXXXXXXXXTRGGDTSASNRGTWGQSST------NAFGVVGSNSAPTPYAPRHGVRIHAGAGPGGSVVYQRYCHMYSEGELEGLVESVDGLRVVKSYYDRSNWCVVAERK 575
            +E+ HVQ+VY  IAPH+S TRYKPWP VE+FL  +   S+ AD GCGNGKYLG +      +GSD S NL+ +C ++    E +  D + LPYR+  FD VISIAV+HHFSS  RR+ A+ EL R +RPGG +L++ WA+EQE  S+R F  QDV VPW+L  +    K   +  NG       +  Q  Q                 GDT   +R T                 +   S P   AP+  V + +G     ++VYQRY H++ +GEL+ +VE+  GL V KS YDR NW ++A +K
Sbjct:   12 LERTHVQSVYQAIAPHFSATRYKPWPVVEDFLGAIPDGSIGADVGCGNGKYLGVNPKL-LTIGSDMSSNLVGICAQR--GFEAMVCDNLHLPYRTNCFDFVISIAVIHHFSSFERRVAAVKELVRTIRPGGKILIFVWALEQEDGSKRKFEKQDVFVPWNLDAKTFAGKSKSVAANGGRRKGKKNQGQDGQDSAAVVEDK--------GDTKEVDRTTPSSPEVANPNVAKTIEALSLESNPEDAAPK--VVVKSG---NENIVYQRYYHVFVKGELDQVVEAAGGLDVNKSGYDRDNWYIIATKK 301          

BLAST of mRNA_M-pyrifera_M_contig1072.1542.1 vs. uniprot
Match: A0A1Q9DEK4_SYMMI (Alkbh8 protein n=2 Tax=Symbiodinium TaxID=2949 RepID=A0A1Q9DEK4_SYMMI)

HSP 1 Score: 199 bits (505), Expect = 1.630e-55
Identity = 121/308 (39.29%), Postives = 159/308 (51.62%), Query Frame = 0

Query:  272 KTPLIEKEHVQAVYDTIAPHWSHTRYKPWPRVEEFLRELEAFSLVADAGCGNGKYLG-CDAVGGWMLGSDASVNLLQVCKEKFPNAEVLASDCMRLPYRSGVFDAVISIAVLHHFSSEARRLRALSELCRLLRPGGLMLVYAWAIEQEQD---SRRSFAAQDVLVPWHLGDQHNHQKKTDLPRNGASANNASSNQQTCQTDXXXXXXXXXXXXTRGGDTSASNRGTWGQSSTNAFGVVGSNSAPTPYAPRHGVRIHAGAGPGGSVVYQRYCHMYSEGELEGLVESVDGLRVVKSYYDRSNWCVVAERK 575
            +TP +E E+V  VYD IA HW+HTRYK WP+VE F+R L   SLVAD GCGNGK L      G + + SD S  L ++  ++      + SDC+  P R+G FDAV+SIAVLHH S+E RR++AL E  RLLRPGG +LVY W+ EQ+     SR  F AQDVLVPW          +T   +  A   +     +T   D            + GGD      G+W +                                    V QRYCH+Y EGEL+ L+E +  L V++SY+D  NWC VA ++
Sbjct:   36 RTPTLEVENVHKVYDAIASHWNHTRYKAWPKVEAFIRGLPRGSLVADLGCGNGKNLPHVKEAGCFPVASDISGPLAEIAAKEH-GVCCMVSDCLCAPLRNGSFDAVLSIAVLHHLSTEPRRVQALREAARLLRPGGQLLVYCWSYEQDDQRSRSRHRFQAQDVLVPWSF--------RTPGVKKAAGGTSKGGRDETLLKD------------SAGGDEDG---GSWEEKPP---------------------------------VCQRYCHVYREGELQELLEQLPELEVLESYFDTGNWCAVARKR 286          

BLAST of mRNA_M-pyrifera_M_contig1072.1542.1 vs. uniprot
Match: A0A507DWG0_9FUNG (Methyltransf_11 domain-containing protein n=1 Tax=Powellomyces hirtus TaxID=109895 RepID=A0A507DWG0_9FUNG)

HSP 1 Score: 194 bits (493), Expect = 1.280e-54
Identity = 116/301 (38.54%), Postives = 154/301 (51.16%), Query Frame = 0

Query:  274 PLIEKEHVQAVYDTIAPHWSHTRYKPWPRVEEFLRELEAFSLVADAGCGNGKYLGCDAVGGWMLGSDASVNLLQVCKEKFPNAEVLASDCMRLPYRSGVFDAVISIAVLHHFSSEARRLRALSELCRLLRPGGLMLVYAWAIEQEQDSRRSFAAQDVLVPWHLGDQHNHQKKTDLPRNGASANNASSNQQTCQTDXXXXXXXXXXXXTRGGDTSASNRGTWGQSSTNAFGVVGSNSAPTPYAPRHGVRIHAGAGPGGSVVYQRYCHMYSEGELEGLVESVDGLRVVKSYYDRSNWCVVAER 574
            P +E+ HV  VY+ IA H+S TRYKPWP V++FLR LE  S+ AD GCGNGKYLG +    + +GSD S  L+ +C+E+    E +  D ++LPYR   FD + SIAV+HH SS  RRL A++EL R+LRPGG +L++ WA EQE+ SRR F  QDV VPW +       K   LP   A+AN   +   T + +                                                            GG VVYQRY H++ EGELE +V   +G  +  S YDR NW V+  +
Sbjct:    7 PQLEQTHVHNVYNIIAGHFSATRYKPWPVVDDFLRGLEKGSVGADVGCGNGKYLGVNP-DVFTIGSDRSDKLIDICRER--GFEAMVCDNLKLPYRDNCFDFLTSIAVIHHMSSPDRRLMAVNELIRVLRPGGRLLIFVWAFEQEKRSRRRFEEQDVFVPWKMP------KNVYLPEGSAAANTTPNT--TVEEE------------------------------------------------------------GGDVVYQRYYHLFKEGELEEIVGRSEGAIIDMSGYDRDNWYVIVTK 236          

BLAST of mRNA_M-pyrifera_M_contig1072.1542.1 vs. uniprot
Match: A0A813EA28_POLGL (Hypothetical protein n=2 Tax=Polarella glacialis TaxID=89957 RepID=A0A813EA28_POLGL)

HSP 1 Score: 196 bits (499), Expect = 1.860e-53
Identity = 122/307 (39.74%), Postives = 154/307 (50.16%), Query Frame = 0

Query:  273 TPLIEKEHVQAVYDTIAPHWSHTRYKPWPRVEEFLRELEAFSLVADAGCGNGKYLG-CDAVGGWMLGSDASVNLLQVCKEKFPNAEVLASDCMRLPYRSGVFDAVISIAVLHHFSSEARRLRALSELCRLLRPGGLMLVYAWAIEQEQD---SRRSFAAQDVLVPWHLGDQHNHQKKTDLPRNGASANNASSNQQTCQTDXXXXXXXXXXXXTRGGDTSASNRGTWGQSSTNAFGVVGSNSAPTPYAPRHGVRIHAGAGPGGSVVYQRYCHMYSEGELEGLVESVDGLRVVKSYYDRSNWCVVAERK 575
            TP +E E+V  VYD IA HW+HTRYK WPRVE F+R L   S+VAD GCGNGK L    A GG+ + SD    L+++  E+   A  L +DC+  P+RSG FDA ISIAVLHH S+E RR++AL E  R+LRPGGL LVY W+ EQ+     SR  F AQDVLVPW          +T   +       A +  Q    +                                A       SA     P                V QRYCH+Y EGEL+ L+  V  L VV+ Y+D  NWC ++ R+
Sbjct:  148 TPALEAENVHRVYDAIADHWNHTRYKAWPRVEGFIRSLPFGSVVADLGCGNGKNLPHVKAAGGFAVASDMCAPLVRIAAEEH-GACGLVADCLVTPFRSGGFDAAISIAVLHHLSTEPRRVQALREAARVLRPGGLFLVYCWSYEQDDQLSRSRHRFVAQDVLVPWSF--------RTPGIKKVKGQEKAEAEAQALARE--------------------------------AAAEADPGSADWQEQPP---------------VCQRYCHVYCEGELDKLLAQVPELEVVEIYFDTGNWCAISRRR 398          

BLAST of mRNA_M-pyrifera_M_contig1072.1542.1 vs. uniprot
Match: UPI0009A3E47C (alkylated DNA repair protein alkB homolog 8-like n=1 Tax=Rhincodon typus TaxID=259920 RepID=UPI0009A3E47C)

HSP 1 Score: 194 bits (494), Expect = 8.630e-53
Identity = 118/310 (38.06%), Postives = 172/310 (55.48%), Query Frame = 0

Query:  270 DGKTPLIEKEHVQAVYDTIAPHWSHTRYKPWPRVEEFLRELEAFSLVADAGCGNGKYLGCDAVGGWMLGSDASVNLLQVCKEKFPNAEVLASDCMRLPYRSGVFDAVISIAVLHHFSSEARRLRALSELCRLLRPGGLMLVYAWAIEQEQDSRRSFAAQDVLVPWHLGDQHNHQKKTDLPRNGASANNASSNQQTCQTDXXXXXXXXXXXXTRGGDTSASNRGTWGQSSTNAFGVVGSNSAPTPYAPRHGVRIHAGA-----GPGGSVVYQRYCHMYSEGELEGLVESVDGLRVVKSYYDRSNWCVVAER 574
            DG    +E ++V  VYD IA H+S TR+ PWP+V EFL+ L A + VAD GCGNGKYLG +    +M+G D S +L+ +C+++  N EV   D + +P RSGVFDA ISIAV+HHFS+E RRL A+ E+ RLLRPGG  L+Y WA+EQE+D+ +S    +             +KKT   +N  +    + +Q   Q D                + + +         TN      S     P+  + GV++ + +     GP    V+ RY H++S GELE L   +  +R+++SYYD+ NWCV+ E+
Sbjct:  111 DGDASKLEHKYVHDVYDEIAGHFSSTRHTPWPKVTEFLKALPAGAFVADVGCGNGKYLGVNK-DLFMVGCDHSKSLVHICRKR--NFEVFICDGLLVPLRSGVFDACISIAVVHHFSTEGRRLSAIKEIVRLLRPGGKGLIYVWAMEQERDNIKSKYLME-------------RKKTTKRQNCTTMPIVNEDQDINQVDLAHFSQLEDQNKDIVNEQNCAKLPVH----TNRTSFE-SQDLLVPWHLKAGVKVDSASSLPPTGPKNITVFHRYYHVFSAGELENLCSCIPNMRIIESYYDQGNWCVIIEK 399          

BLAST of mRNA_M-pyrifera_M_contig1072.1542.1 vs. uniprot
Match: A0A813L7Z6_POLGL (Hypothetical protein (Fragment) n=1 Tax=Polarella glacialis TaxID=89957 RepID=A0A813L7Z6_POLGL)

HSP 1 Score: 196 bits (499), Expect = 1.230e-52
Identity = 122/307 (39.74%), Postives = 154/307 (50.16%), Query Frame = 0

Query:  273 TPLIEKEHVQAVYDTIAPHWSHTRYKPWPRVEEFLRELEAFSLVADAGCGNGKYLG-CDAVGGWMLGSDASVNLLQVCKEKFPNAEVLASDCMRLPYRSGVFDAVISIAVLHHFSSEARRLRALSELCRLLRPGGLMLVYAWAIEQEQD---SRRSFAAQDVLVPWHLGDQHNHQKKTDLPRNGASANNASSNQQTCQTDXXXXXXXXXXXXTRGGDTSASNRGTWGQSSTNAFGVVGSNSAPTPYAPRHGVRIHAGAGPGGSVVYQRYCHMYSEGELEGLVESVDGLRVVKSYYDRSNWCVVAERK 575
            TP +E E+V  VYD IA HW+HTRYK WPRVE F+R L   S+VAD GCGNGK L    A GG+ + SD    L+++  E+   A  L +DC+  P+RSG FDA ISIAVLHH S+E RR++AL E  R+LRPGGL LVY W+ EQ+     SR  F AQDVLVPW          +T   +       A +  Q    +                                A       SA     P                V QRYCH+Y EGEL+ L+  V  L VV+ Y+D  NWC ++ R+
Sbjct:  230 TPALEAENVHRVYDAIADHWNHTRYKAWPRVEGFIRSLPFGSVVADLGCGNGKNLPHVKAAGGFAVASDMCAPLVRIAAEEH-GACGLVADCLVTPFRSGGFDAAISIAVLHHLSTEPRRVQALREAARVLRPGGLFLVYCWSYEQDDQLSRSRHRFVAQDVLVPWSF--------RTPGIKKVKGQEKAEAEAQALARE--------------------------------AAAEADPGSADWQEQPP---------------VCQRYCHVYCEGELDKLLAQVPELEVVEIYFDTGNWCAISRRR 480          

BLAST of mRNA_M-pyrifera_M_contig1072.1542.1 vs. uniprot
Match: A0A482RAD7_9ARCH (Class I SAM-dependent methyltransferase (Fragment) n=1 Tax=archaeon TaxID=1906665 RepID=A0A482RAD7_9ARCH)

HSP 1 Score: 192 bits (488), Expect = 1.270e-52
Identity = 119/319 (37.30%), Postives = 171/319 (53.61%), Query Frame = 0

Query:  274 PLIEKEHVQAVYDTIAPHWSHTRYKPWPRVEEFLRELEAFSLVADAGCGNGKYLGCDAVGGWMLGSDASVNLLQVCKEKFPNAEVLASDCMRLPYRSGVFDAVISIAVLHHFSSEARRLRALSELCRLLRPGGLMLVYAWAIEQEQDSRRSFAAQDVLVPWHLGDQHNHQKKTDLPRNGASANNASSNQQTCQTDXXXXXXXXXXXXTRGGDTSASNRGTWGQSSTNAFGVVGSNSAPTPYAPRHGVRIHAGAG-----PGGSVVYQRYCHMYSEGELEGLVESVDG------------LRVVKSYYDRSNWCVVAERK 575
            P +E+ HV A+YD IAPH+S TR+ PWP VE F++ L   S+V D GCGNGKY+G +  G  ++G D S  LL++C ++    + L +D + LP+ SG  D V++IAVLHH S+ ARR   + EL RLLRPGG + V AWA EQ  DSRR+F +QDV VPW+L  ++       L   GAS + A+  Q T +              +   D SAS  G    S+ +  G                      AG       G+V++QRYCH++  GELE L+ +               + V ++++D+ NWC++A R+
Sbjct:   47 PDVERLHVHALYDAIAPHFSATRHSPWPAVETFVKALPVGSVVLDIGCGNGKYMGLNP-GALVIGMDRSEGLLRICADR--GYDTLQADAVSLPFTSGCADVVLNIAVLHHISTAARRESLMRELVRLLRPGGTLFVQAWAFEQGPDSRRTFDSQDVFVPWNLSMRY-------LGGGGASTD-AALPQDTTRA-------------STASDDSASGVG----SAAHPSGXXXXXXXXXXXXXXXXXXXLEAAGGVVNAKAGTVLFQRYCHVFKGGELEQLLHAATSSWPATEVARAPEMEVTRAWWDKDNWCIIAHRR 337