prot_M-pyrifera_M_contig105465.1171.1 (polypeptide) Macrocystis pyrifera P11B4 male

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview
NamemRNA_M-pyrifera_M_contig105465.1171.1
Unique Nameprot_M-pyrifera_M_contig105465.1171.1
Typepolypeptide
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Sequence length211
Homology
BLAST of mRNA_M-pyrifera_M_contig105465.1171.1 vs. uniprot
Match: A0A3D5DGD8_9GAMM (3-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A3D5DGD8_9GAMM)

HSP 1 Score: 375 bits (963), Expect = 7.980e-128
Identity = 186/211 (88.15%), Postives = 199/211 (94.31%), Query Frame = 0
Query:    1 EQAYQSYLDGGARKVSPFFVPGNIINMVAGNLSIRYGFKGPNYAMVTACSTGAHCISNAARMIERGDLDVMIAGGAEFATSYTGLGGFASAKALSTRNDDPEAASRPWDRDRDGFVLSDGAGIVVIEELEHARKRGARIYAELAGSGMSSDAYHITAPSPDGEGAAYCMELAATDAGIDASEVQYINAHGTSTPAGDKAETDAVKRFFGDH 211
            EQAYQ+YLDGGARKVSPFFVPGNIINMVAGNLSIRYGFKGPNYA+VTACSTGAHCISNAARMIERGD+DVMIAGG+EFATSYTGLGGFASAKALSTRND PE ASRPWD +RDGFVLS+GAGIVV+EELEHA +RGARIYAEL G+GMSSDA+HITAPS DGEGAAYCMELA  DAG+   +V YINAHGTSTPAGDKAE+DAVKRFFGDH
Sbjct:  115 EQAYQAYLDGGARKVSPFFVPGNIINMVAGNLSIRYGFKGPNYALVTACSTGAHCISNAARMIERGDVDVMIAGGSEFATSYTGLGGFASAKALSTRNDAPEQASRPWDLERDGFVLSEGAGIVVLEELEHAERRGARIYAELLGAGMSSDAFHITAPSTDGEGAAYCMELAIKDAGLRKEDVDYINAHGTSTPAGDKAESDAVKRFFGDH 325          
BLAST of mRNA_M-pyrifera_M_contig105465.1171.1 vs. uniprot
Match: A0A523P129_9PROT (3-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Tax=Proteobacteria bacterium TaxID=1977087 RepID=A0A523P129_9PROT)

HSP 1 Score: 375 bits (963), Expect = 8.240e-128
Identity = 184/211 (87.20%), Postives = 200/211 (94.79%), Query Frame = 0
Query:    1 EQAYQSYLDGGARKVSPFFVPGNIINMVAGNLSIRYGFKGPNYAMVTACSTGAHCISNAARMIERGDLDVMIAGGAEFATSYTGLGGFASAKALSTRNDDPEAASRPWDRDRDGFVLSDGAGIVVIEELEHARKRGARIYAELAGSGMSSDAYHITAPSPDGEGAAYCMELAATDAGIDASEVQYINAHGTSTPAGDKAETDAVKRFFGDH 211
            E+AYQSYLDGG RKVSPFFVPGNIINMVAGNLSIRYGFKGPNYAMVTACSTGAHCI++AARMIER D+DVMIAGGAEFATSYTGLGGFASAKALSTRNDDPE ASRPWD +RDGFVLSDGAGI+V+EE+E+A++RGARIYAELAGSGMSSDAYHITAP+PDGEGAA+CMELA  DA  +  +V YINAHGTSTPAGDKAETDAVKR+FGDH
Sbjct:  116 EKAYQSYLDGGPRKVSPFFVPGNIINMVAGNLSIRYGFKGPNYAMVTACSTGAHCIASAARMIERDDVDVMIAGGAEFATSYTGLGGFASAKALSTRNDDPEGASRPWDSERDGFVLSDGAGIIVLEEMEYAKRRGARIYAELAGSGMSSDAYHITAPTPDGEGAAHCMELAVADAEFNRDDVDYINAHGTSTPAGDKAETDAVKRYFGDH 326          
BLAST of mRNA_M-pyrifera_M_contig105465.1171.1 vs. uniprot
Match: A0A7C5PS12_9GAMM (3-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A7C5PS12_9GAMM)

HSP 1 Score: 331 bits (849), Expect = 1.940e-110
Identity = 163/211 (77.25%), Postives = 185/211 (87.68%), Query Frame = 0
Query:    1 EQAYQSYLDGGARKVSPFFVPGNIINMVAGNLSIRYGFKGPNYAMVTACSTGAHCISNAARMIERGDLDVMIAGGAEFATSYTGLGGFASAKALSTRNDDPEAASRPWDRDRDGFVLSDGAGIVVIEELEHARKRGARIYAELAGSGMSSDAYHITAPSPDGEGAAYCMELAATDAGIDASEVQYINAHGTSTPAGDKAETDAVKRFFGDH 211
            EQAY++YL GG RK+SPFFVP NIINMV+GNLSI YG KGPN ++V+AC+TG H I +A RMI+ G +DVM+AGGAE ATS TGLGGFA+A+ALSTRNDDP+AASRPWDRDRDGFVLSDGAG+VV+EELEHAR RGARIYAEL GSGM+SDAYH+T+PSP GEGAA CM+LA  DAGID  +V YINAHGTSTPAGD AET AVKR FGDH
Sbjct:  120 EQAYEAYLKGGPRKISPFFVPANIINMVSGNLSIMYGLKGPNLSLVSACATGTHAIGDAMRMIQYGTVDVMVAGGAEMATSPTGLGGFAAARALSTRNDDPQAASRPWDRDRDGFVLSDGAGVVVLEELEHARARGARIYAELIGSGMNSDAYHMTSPSPGGEGAAECMQLALEDAGIDPEQVDYINAHGTSTPAGDVAETKAVKRAFGDH 330          
BLAST of mRNA_M-pyrifera_M_contig105465.1171.1 vs. uniprot
Match: A0A3M1DKY3_9GAMM (3-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A3M1DKY3_9GAMM)

HSP 1 Score: 330 bits (845), Expect = 6.920e-110
Identity = 162/210 (77.14%), Postives = 181/210 (86.19%), Query Frame = 0
Query:    1 EQAYQSYLDGGARKVSPFFVPGNIINMVAGNLSIRYGFKGPNYAMVTACSTGAHCISNAARMIERGDLDVMIAGGAEFATSYTGLGGFASAKALSTRNDDPEAASRPWDRDRDGFVLSDGAGIVVIEELEHARKRGARIYAELAGSGMSSDAYHITAPSPDGEGAAYCMELAATDAGIDASEVQYINAHGTSTPAGDKAETDAVKRFFGD 210
            EQ YQ++LDGG RK+SPFFVP NIINMV+GNLSIRYGFKGPNYA+VTAC+TG HCI +AAR I  G+ DVMIAGG+E ATS TGLGGF +A+ALSTRNDDP+ ASRPWDRDRDGFVL DG G++V+EELEHAR+RGARIYAELAG GMS+DAYHITAPS DGEGAA CM LA  DAG+    V YINAHGTSTPAGD AET AVKR FG+
Sbjct:  117 EQGYQAFLDGGPRKISPFFVPSNIINMVSGNLSIRYGFKGPNYAIVTACATGTHCIGDAARKIRYGEADVMIAGGSEMATSPTGLGGFCAARALSTRNDDPQRASRPWDRDRDGFVLGDGGGVLVLEELEHARRRGARIYAELAGFGMSADAYHITAPSEDGEGAARCMALALEDAGLGPDAVDYINAHGTSTPAGDVAETQAVKRLFGE 326          
BLAST of mRNA_M-pyrifera_M_contig105465.1171.1 vs. uniprot
Match: A0A1S9D3Z6_9GAMM (3-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Tax=Chromatiales bacterium USCg_Taylor TaxID=1934255 RepID=A0A1S9D3Z6_9GAMM)

HSP 1 Score: 321 bits (822), Expect = 1.980e-106
Identity = 151/203 (74.38%), Postives = 180/203 (88.67%), Query Frame = 0
Query:    9 DGGARKVSPFFVPGNIINMVAGNLSIRYGFKGPNYAMVTACSTGAHCISNAARMIERGDLDVMIAGGAEFATSYTGLGGFASAKALSTRNDDPEAASRPWDRDRDGFVLSDGAGIVVIEELEHARKRGARIYAELAGSGMSSDAYHITAPSPDGEGAAYCMELAATDAGIDASEVQYINAHGTSTPAGDKAETDAVKRFFGDH 211
            DGG +K+SPF++P NIINM+AGNLSI++G +GPN+A+VTACSTG HCI +AAR+IERGD DVM+AGGAE AT+ TGLGGF SA+ALSTRNDDPE ASRPWDRDRDGFVLSDGAG++V+EE EHA++RGARIY E+ G G+++DAYH+TAPSP G+GAA CM+LA  DA IDA+E+ YINAHGTSTPAGDKAETDAVK  F DH
Sbjct:  124 DGGPKKISPFYIPSNIINMIAGNLSIKFGLRGPNFAIVTACSTGTHCIGDAARIIERGDADVMVAGGAEMATTPTGLGGFCSARALSTRNDDPETASRPWDRDRDGFVLSDGAGVIVLEEYEHAKRRGARIYVEVTGYGINADAYHMTAPSPGGQGAARCMQLALRDAAIDANEIDYINAHGTSTPAGDKAETDAVKLVFKDH 326          
BLAST of mRNA_M-pyrifera_M_contig105465.1171.1 vs. uniprot
Match: A0A7C1YF59_9GAMM (3-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Tax=Sedimenticola sp. TaxID=1940285 RepID=A0A7C1YF59_9GAMM)

HSP 1 Score: 316 bits (810), Expect = 1.380e-104
Identity = 158/211 (74.88%), Postives = 179/211 (84.83%), Query Frame = 0
Query:    1 EQAYQSYLDGGARKVSPFFVPGNIINMVAGNLSIRYGFKGPNYAMVTACSTGAHCISNAARMIERGDLDVMIAGGAEFATSYTGLGGFASAKALSTRNDDPEAASRPWDRDRDGFVLSDGAGIVVIEELEHARKRGARIYAELAGSGMSSDAYHITAPSPDGEGAAYCMELAATDAGIDASEVQYINAHGTSTPAGDKAETDAVKRFFGDH 211
            E +Y  YL GG RK+SPFFVP NIINMVAGNLSI +G KGPN ++V+ACSTG+H I  AARMI+ G +DVM+AGGAE ATS TGLGGFA+A+ALSTRNDDPE ASRPWDRDRDGFVLSDGAG+VV+EELEHA KRGA IYAELAG GM+SDA+H+TAPSP+GEGA+ CM LA  D G++  EV YINAHGTSTPAGD AET AVK  FGDH
Sbjct:  116 ENSYGDYLKGGPRKISPFFVPSNIINMVAGNLSIMHGLKGPNISIVSACSTGSHNIGEAARMIQYGGVDVMVAGGAEMATSPTGLGGFAAARALSTRNDDPEGASRPWDRDRDGFVLSDGAGVVVLEELEHALKRGATIYAELAGVGMNSDAFHMTAPSPNGEGASECMLLALEDGGVNPDEVDYINAHGTSTPAGDLAETQAVKAAFGDH 326          
BLAST of mRNA_M-pyrifera_M_contig105465.1171.1 vs. uniprot
Match: A0A7Z0VJV0_9GAMM (3-oxoacyl-[acyl-carrier-protein] synthase 2 n=7 Tax=Gammaproteobacteria TaxID=1236 RepID=A0A7Z0VJV0_9GAMM)

HSP 1 Score: 315 bits (806), Expect = 5.400e-104
Identity = 157/211 (74.41%), Postives = 178/211 (84.36%), Query Frame = 0
Query:    1 EQAYQSYLDGGARKVSPFFVPGNIINMVAGNLSIRYGFKGPNYAMVTACSTGAHCISNAARMIERGDLDVMIAGGAEFATSYTGLGGFASAKALSTRNDDPEAASRPWDRDRDGFVLSDGAGIVVIEELEHARKRGARIYAELAGSGMSSDAYHITAPSPDGEGAAYCMELAATDAGIDASEVQYINAHGTSTPAGDKAETDAVKRFFGDH 211
            E +YQ+YLDGG RK+SPFFVP +IINMV+GNLSI YG KGPNY++V+ACS+GAH I+ AA MI  G  DVMIAGGAE ATS  GLGGFA+A+ALS RNDDP+ ASRPWD+DRDGFVLSDGAG+VV+EE E A+ RGA+IYAELAG GM+SDAYH+TAPS DG GAA CMELA  D GI+  EV YINAHGTSTPAGD AET AVKR FGDH
Sbjct:  116 ENSYQAYLDGGPRKISPFFVPSSIINMVSGNLSIMYGLKGPNYSIVSACSSGAHSIAEAAMMIRHGRTDVMIAGGAEMATSPVGLGGFAAARALSRRNDDPQGASRPWDKDRDGFVLSDGAGVVVLEEYEQAKARGAKIYAELAGIGMNSDAYHMTAPSVDGSGAAKCMELALQDGGINVDEVDYINAHGTSTPAGDVAETMAVKRAFGDH 326          
BLAST of mRNA_M-pyrifera_M_contig105465.1171.1 vs. uniprot
Match: A0A3B1AUS1_9ZZZZ (3-oxoacyl-[acyl-carrier-protein] synthase, KASII n=1 Tax=hydrothermal vent metagenome TaxID=652676 RepID=A0A3B1AUS1_9ZZZZ)

HSP 1 Score: 313 bits (802), Expect = 2.250e-103
Identity = 155/211 (73.46%), Postives = 178/211 (84.36%), Query Frame = 0
Query:    1 EQAYQSYLDGGARKVSPFFVPGNIINMVAGNLSIRYGFKGPNYAMVTACSTGAHCISNAARMIERGDLDVMIAGGAEFATSYTGLGGFASAKALSTRNDDPEAASRPWDRDRDGFVLSDGAGIVVIEELEHARKRGARIYAELAGSGMSSDAYHITAPSPDGEGAAYCMELAATDAGIDASEVQYINAHGTSTPAGDKAETDAVKRFFGDH 211
            E ++  YL GG RK+SPFFVP NIINMVAGNLSI +G KGPN ++V+ACSTG H I  AARMI  G +D M+AGGAE ATS TGLGGFA+A+ALSTRNDDP+ ASRPWDRDRDGFVLSDGAG++VIEE+E A+KRGA+IYAEL G GM+SDAYH+T+PSP+GEGAA CM LA  DAG++A EV YINAHGTSTPAGD AET AVK  FGDH
Sbjct:  116 ENSFGDYLRGGPRKISPFFVPSNIINMVAGNLSIMHGLKGPNISIVSACSTGTHNIGEAARMIRYGGVDAMVAGGAEMATSPTGLGGFAAARALSTRNDDPQGASRPWDRDRDGFVLSDGAGVIVIEEMECAKKRGAKIYAELIGVGMNSDAYHMTSPSPNGEGAAQCMRLALQDAGVNAEEVDYINAHGTSTPAGDLAETQAVKAAFGDH 326          
BLAST of mRNA_M-pyrifera_M_contig105465.1171.1 vs. uniprot
Match: A0A2E2D420_9GAMM (3-oxoacyl-[acyl-carrier-protein] synthase 2 n=8 Tax=root TaxID=1 RepID=A0A2E2D420_9GAMM)

HSP 1 Score: 313 bits (801), Expect = 3.090e-103
Identity = 155/210 (73.81%), Postives = 174/210 (82.86%), Query Frame = 0
Query:    1 EQAYQSYLDGGARKVSPFFVPGNIINMVAGNLSIRYGFKGPNYAMVTACSTGAHCISNAARMIERGDLDVMIAGGAEFATSYTGLGGFASAKALSTRNDDPEAASRPWDRDRDGFVLSDGAGIVVIEELEHARKRGARIYAELAGSGMSSDAYHITAPSPDGEGAAYCMELAATDAGIDASEVQYINAHGTSTPAGDKAETDAVKRFFGD 210
            E  Y SYL+GG RK+SPFFVP NIINM++GNLSI YG KGPN A+VTACSTG HCIS A RMI+ GD DVMIAGGAE ATS TGLGGFA+A+ALSTRNDDP AASRPWD+DRDGFVL DGAG++V+EE EHA KRGA IYAEL GSGMSSDAYH+T+PS  GEGAA CM  A  DAG++   + YINAHGTSTPAGD AET A+K+  GD
Sbjct:  116 EAGYDSYLNGGPRKISPFFVPSNIINMISGNLSIMYGMKGPNTAIVTACSTGTHCISAAGRMIQYGDADVMIAGGAEMATSKTGLGGFAAARALSTRNDDPAAASRPWDKDRDGFVLGDGAGVIVLEEYEHAVKRGATIYAELVGSGMSSDAYHMTSPSEGGEGAARCMVNAIKDAGLNPEAIDYINAHGTSTPAGDAAETQAIKKALGD 325          
BLAST of mRNA_M-pyrifera_M_contig105465.1171.1 vs. uniprot
Match: A0A7C5J8L5_9GAMM (3-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A7C5J8L5_9GAMM)

HSP 1 Score: 313 bits (801), Expect = 3.090e-103
Identity = 154/211 (72.99%), Postives = 175/211 (82.94%), Query Frame = 0
Query:    1 EQAYQSYLDGGARKVSPFFVPGNIINMVAGNLSIRYGFKGPNYAMVTACSTGAHCISNAARMIERGDLDVMIAGGAEFATSYTGLGGFASAKALSTRNDDPEAASRPWDRDRDGFVLSDGAGIVVIEELEHARKRGARIYAELAGSGMSSDAYHITAPSPDGEGAAYCMELAATDAGIDASEVQYINAHGTSTPAGDKAETDAVKRFFGDH 211
            E+ Y SYL GG RK+SPFF+P NIINM++GNLSI YG KGPN A+VTACS+  HCI +AAR+IE GD DVM+AGGAE ATS TGLGGF SA+ALSTRNDDP+ ASRPWDRDRDGFVLSDGAGIVV+EELEHAR+RGARIYAE+ G GMS DA+H+TAP PDG+GA  CM  A  +AGI    + YINAHGTSTPAGD AET A+K  FGDH
Sbjct:  117 EKGYDSYLKGGPRKISPFFIPSNIINMLSGNLSIMYGMKGPNIAIVTACSSATHCIGDAARLIEYGDADVMVAGGAEMATSPTGLGGFCSARALSTRNDDPQGASRPWDRDRDGFVLSDGAGIVVLEELEHARRRGARIYAEIIGYGMSGDAHHMTAPPPDGDGARRCMVNAMRNAGIAPDAIDYINAHGTSTPAGDIAETRAIKSAFGDH 327          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig105465.1171.1 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3D5DGD8_9GAMM7.980e-12888.153-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Ta... [more]
A0A523P129_9PROT8.240e-12887.203-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Ta... [more]
A0A7C5PS12_9GAMM1.940e-11077.253-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Ta... [more]
A0A3M1DKY3_9GAMM6.920e-11077.143-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Ta... [more]
A0A1S9D3Z6_9GAMM1.980e-10674.383-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Ta... [more]
A0A7C1YF59_9GAMM1.380e-10474.883-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Ta... [more]
A0A7Z0VJV0_9GAMM5.400e-10474.413-oxoacyl-[acyl-carrier-protein] synthase 2 n=7 Ta... [more]
A0A3B1AUS1_9ZZZZ2.250e-10373.463-oxoacyl-[acyl-carrier-protein] synthase, KASII n... [more]
A0A2E2D420_9GAMM3.090e-10373.813-oxoacyl-[acyl-carrier-protein] synthase 2 n=8 Ta... [more]
A0A7C5J8L5_9GAMM3.090e-10372.993-oxoacyl-[acyl-carrier-protein] synthase 2 n=1 Ta... [more]

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InterPro
Analysis Name: InterProScan on OGS1.0 of Macrocystis pyrifera male
Date Performed: 2022-09-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020841Polyketide synthase, beta-ketoacyl synthase domainSMARTSM00825PKS_KScoord: 2..211
e-value: 9.2E-5
score: -195.1
IPR016039Thiolase-likeGENE3D3.40.47.10coord: 1..140
e-value: 1.9E-51
score: 177.2
IPR016039Thiolase-likeGENE3D3.40.47.10coord: 142..211
e-value: 1.2E-23
score: 85.6
IPR016039Thiolase-likeSUPERFAMILY53901Thiolase-likecoord: 20..203
IPR014030Beta-ketoacyl synthase, N-terminalPFAMPF00109ketoacyl-syntcoord: 7..132
e-value: 1.6E-35
score: 122.9
IPR014031Beta-ketoacyl synthase, C-terminalPFAMPF02801Ketoacyl-synt_Ccoord: 140..211
e-value: 2.5E-21
score: 75.9
IPR000794Beta-ketoacyl synthasePANTHERPTHR11712POLYKETIDE SYNTHASE-RELATEDcoord: 2..211
IPR018201Beta-ketoacyl synthase, active sitePROSITEPS00606B_KETOACYL_SYNTHASEcoord: 40..56

Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig105465contigM-pyrifera_M_contig105465:2..634 +
Analyses
This polypeptide is derived from or has results from the following analyses
Analysis NameDate Performed
InterProScan on OGS1.0 of Macrocystis pyrifera male2022-09-29
Diamond blastp: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-16
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Relationships

This polypeptide derives from the following mRNA feature(s):

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig105465.1171.1mRNA_M-pyrifera_M_contig105465.1171.1Macrocystis pyrifera P11B4 malemRNAM-pyrifera_M_contig105465 2..634 +


Sequences
The following sequences are available for this feature:

polypeptide sequence

>prot_M-pyrifera_M_contig105465.1171.1 ID=prot_M-pyrifera_M_contig105465.1171.1|Name=mRNA_M-pyrifera_M_contig105465.1171.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=211bp
EQAYQSYLDGGARKVSPFFVPGNIINMVAGNLSIRYGFKGPNYAMVTACS
TGAHCISNAARMIERGDLDVMIAGGAEFATSYTGLGGFASAKALSTRNDD
PEAASRPWDRDRDGFVLSDGAGIVVIEELEHARKRGARIYAELAGSGMSS
DAYHITAPSPDGEGAAYCMELAATDAGIDASEVQYINAHGTSTPAGDKAE
TDAVKRFFGDH
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Annotated Terms
The following terms have been associated with this polypeptide:
Vocabulary: INTERPRO
TermDefinition
IPR020841PKS_Beta-ketoAc_synthase_dom
IPR016039Thiolase-like
IPR014030Ketoacyl_synth_N
IPR014031Ketoacyl_synth_C
IPR000794Beta-ketoacyl_synthase
IPR018201Ketoacyl_synth_AS