mRNA_M-pyrifera_M_contig99743.22924.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig99743.22924.1
Unique NamemRNA_M-pyrifera_M_contig99743.22924.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig99743.22924.1 vs. uniprot
Match: A0A0G4E8B5_VITBC (Uncharacterized protein n=2 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A0G4E8B5_VITBC)

HSP 1 Score: 96.3 bits (238), Expect = 5.450e-21
Identity = 44/95 (46.32%), Postives = 56/95 (58.95%), Query Frame = 1 
Query:   28 PSAAWQKNLEDEDVEHESFSVGIKEGIKLAGLGLRMSKFIRKETREGREPFMDPFGSYDVHSNAGVPCGGIGAGSIGRGYKGDFNSFLLRPGRPE 312
            P   W ++  + D     F     + +   GL  R+ KFIR E R+G  P++DPF     H+N GVPCGG+G GSIGRGY+G FN F LRPGR E
Sbjct:   65 PECCWSRSFAEVDPPVREFQPTFWDAVSQLGLAWRLYKFIRHEKRDGLLPWIDPFAPKKPHTNHGVPCGGLGCGSIGRGYRGAFNRFCLRPGRVE 159
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BLAST of mRNA_M-pyrifera_M_contig99743.22924.1 vs. uniprot
Match: Q54D71_DICDI (Uncharacterized protein n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=Q54D71_DICDI)

HSP 1 Score: 94.4 bits (233), Expect = 2.580e-20
Identity = 45/93 (48.39%), Postives = 59/93 (63.44%), Query Frame = 1 
Query:   25 IPSAAWQKNLEDEDVEHESFSVGIKEGIKLAGLGLRMSKFIRKETREGREPFMDPFGSYDVHSNAGVPCGGIGAGSIGRGYKGDFNSFLLRPG 303
            IP  AW + L++   EH+ F V + +G+KL G+G RM K+++KET  GR P MDPF         GVP GGIG+GSI RG+KGDF  + L  G
Sbjct:  368 IPQYAWARRLDNNYPEHKHFQVSMWQGLKLVGMGYRMWKYVKKETSSGRVPIMDPFNLPKPGPIMGVPIGGIGSGSINRGWKGDFVRWNLNNG 460
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BLAST of mRNA_M-pyrifera_M_contig99743.22924.1 vs. uniprot
Match: A0A8J4PXL4_9MYCE (Uncharacterized protein n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4PXL4_9MYCE)

HSP 1 Score: 93.2 bits (230), Expect = 6.540e-20
Identity = 45/93 (48.39%), Postives = 58/93 (62.37%), Query Frame = 1 
Query:   25 IPSAAWQKNLEDEDVEHESFSVGIKEGIKLAGLGLRMSKFIRKETREGREPFMDPFGSYDVHSNAGVPCGGIGAGSIGRGYKGDFNSFLLRPG 303
            IP  AW + L+    +H+ F+V I +G+KL GLG RM K+++KE   GR P MDPF         GVP GGIG+GSI RG+KGDF  + L  G
Sbjct:  350 IPHYAWSRRLDQNFADHKQFNVSIWQGLKLMGLGYRMWKYVKKELTAGRLPIMDPFNLPKPGPIMGVPIGGIGSGSINRGWKGDFVRWNLESG 442
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BLAST of mRNA_M-pyrifera_M_contig99743.22924.1 vs. uniprot
Match: A0A8J5WEF8_ZIZPA (Glyco_hydr_116N domain-containing protein n=1 Tax=Zizania palustris TaxID=103762 RepID=A0A8J5WEF8_ZIZPA)

HSP 1 Score: 84.7 bits (208), Expect = 8.670e-19
Identity = 43/96 (44.79%), Postives = 56/96 (58.33%), Query Frame = 1 
Query:   25 IPSAAWQKNLEDEDVEHESFSVGIKEGIKLAGLGLRMSKFIRKETREGREPFMDPFGSYDVHSNAGVPCGGIGAGSIGRGYKGDFNSFLLRPGRPE 312
            +P   W+  L +   +  SF +  +E  +LAGLGLR+ + I +ET +GR   +DP       S  GVP GGIGAGSIGR YKGDF  + L PG  E
Sbjct:   32 LPVLTWEHKLSNIGYDLPSFRLTWRESFQLAGLGLRLGRHILEETSKGRTAVIDPMKKRIAKSGQGVPLGGIGAGSIGRSYKGDFQRWQLFPGTCE 127
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BLAST of mRNA_M-pyrifera_M_contig99743.22924.1 vs. uniprot
Match: A0A7J7CGI8_TRIWF (Non-lysosomal glucosylceramidase n=2 Tax=Tripterygium wilfordii TaxID=458696 RepID=A0A7J7CGI8_TRIWF)

HSP 1 Score: 89.7 bits (221), Expect = 1.060e-18
Identity = 39/92 (42.39%), Postives = 65/92 (70.65%), Query Frame = 1 
Query:   37 AWQKNLEDEDVEHESFSVGIKEGIKLAGLGLRMSKFIRKETREGREPFMDPFGSYDVHSNAGVPCGGIGAGSIGRGYKGDFNSFLLRPGRPE 312
            AW++ + +E++    FS+ +++ I LA +G+R+ +  R+E+ +G++ F+DPF  + V S+ G+P GGIG+GSIGR YKG+F  + L PGR E
Sbjct:   34 AWKRKINNENMSPLQFSLSLRDTIHLAPIGIRLWRLHREESAKGKDTFIDPFAKHFVTSSHGIPLGGIGSGSIGRSYKGEFQRWQLFPGRCE 125
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BLAST of mRNA_M-pyrifera_M_contig99743.22924.1 vs. uniprot
Match: F1A2R6_DICPU (Uncharacterized protein n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F1A2R6_DICPU)

HSP 1 Score: 88.6 bits (218), Expect = 2.730e-18
Identity = 40/93 (43.01%), Postives = 57/93 (61.29%), Query Frame = 1 
Query:   25 IPSAAWQKNLEDEDVEHESFSVGIKEGIKLAGLGLRMSKFIRKETREGREPFMDPFGSYDVHSNAGVPCGGIGAGSIGRGYKGDFNSFLLRPG 303
            IP  +W + L+    +H+ F V + +G+KL G+G RM K+++KE   GR P MDPF         GVP GGIG+GSI RG++GDF  + +  G
Sbjct:  323 IPQCSWARRLDHNFPDHKHFQVSMWQGLKLVGIGYRMWKYVKKENSSGRVPMMDPFNLPKPGPIMGVPIGGIGSGSINRGWRGDFVRWNMNSG 415
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BLAST of mRNA_M-pyrifera_M_contig99743.22924.1 vs. uniprot
Match: UPI000CED5022 (non-lysosomal glucosylceramidase n=1 Tax=Morus notabilis TaxID=981085 RepID=UPI000CED5022)

HSP 1 Score: 88.2 bits (217), Expect = 3.690e-18
Identity = 45/94 (47.87%), Postives = 59/94 (62.77%), Query Frame = 1 
Query:   22 GIPSA-AWQKNLEDEDVEHESFSVGIKEGIKLAGLGLRMSKFIRKETREGREPFMDPFGSYDVHSNAGVPCGGIGAGSIGRGYKGDFNSFLLRP 300
            GIP+   WQ+ L  E     SFS+ +KE I LA +G R+ + +++E  +GR   M+PF    V SN GVP GGIGAGSIGR YKG+F  + L P
Sbjct:   29 GIPALLTWQRKLNGEGNTPVSFSISLKEVIHLAPIGFRLWRHVQEEAAKGRGALMNPFSKRHVTSNHGVPLGGIGAGSIGRSYKGEFKRWQLFP 122
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BLAST of mRNA_M-pyrifera_M_contig99743.22924.1 vs. uniprot
Match: A0A151Z2R2_9MYCE (Uncharacterized protein n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A151Z2R2_9MYCE)

HSP 1 Score: 87.8 bits (216), Expect = 5.080e-18
Identity = 41/93 (44.09%), Postives = 54/93 (58.06%), Query Frame = 1 
Query:   25 IPSAAWQKNLEDEDVEHESFSVGIKEGIKLAGLGLRMSKFIRKETREGREPFMDPFGSYDVHSNAGVPCGGIGAGSIGRGYKGDFNSFLLRPG 303
            IP  +W + L     +H+ F V   +G+KL G+G RM K+++KE    R P MDPF         GVP GGIG+GS+ RG+KGDF  F L  G
Sbjct:  319 IPQCSWSRKLNQNFPDHKPFQVSAMQGLKLVGIGYRMWKYVKKELTANRVPIMDPFNPPKPGPVMGVPLGGIGSGSVTRGWKGDFVRFNLSGG 411
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BLAST of mRNA_M-pyrifera_M_contig99743.22924.1 vs. uniprot
Match: UPI0005114AE1 (non-lysosomal glucosylceramidase-like isoform X1 n=3 Tax=Pyrus x bretschneideri TaxID=225117 RepID=UPI0005114AE1)

HSP 1 Score: 87.4 bits (215), Expect = 6.830e-18
Identity = 44/94 (46.81%), Postives = 58/94 (61.70%), Query Frame = 1 
Query:   31 SAAWQKNLEDEDVEHESFSVGIKEGIKLAGLGLRMSKFIRKETREGREPFMDPFGSYDVHSNAGVPCGGIGAGSIGRGYKGDFNSFLLRPGRPE 312
            S  WQ+ L  +      F++ +KE I LA +G+R+ + IR+E    RE F+DPF    + S+ GVP GGIGAGSIGR Y GDF  + L PGR E
Sbjct:   47 SLTWQRKLNSKGSAPLPFTLSLKEIIHLAPIGIRLWRHIREEAANEREGFIDPFAKRSLTSSHGVPLGGIGAGSIGRSYSGDFQRWQLFPGRCE 140
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BLAST of mRNA_M-pyrifera_M_contig99743.22924.1 vs. uniprot
Match: A0A498JEH2_MALDO (BUB1 N-terminal domain-containing protein n=1 Tax=Malus domestica TaxID=3750 RepID=A0A498JEH2_MALDO)

HSP 1 Score: 87.4 bits (215), Expect = 6.970e-18
Identity = 43/94 (45.74%), Postives = 60/94 (63.83%), Query Frame = 1 
Query:   31 SAAWQKNLEDEDVEHESFSVGIKEGIKLAGLGLRMSKFIRKETREGREPFMDPFGSYDVHSNAGVPCGGIGAGSIGRGYKGDFNSFLLRPGRPE 312
            S  WQ+ L         F++ +KE + LA +G+R+ + +R+E  +GRE F++PF  + + SN GVP GGIGAGSIGR Y GDF  + L PGR E
Sbjct:  346 SLTWQRKLNSTGNAPLPFNLYLKEIVHLAPIGIRLWRHLREEAAKGREGFINPFVKHHLTSNHGVPLGGIGAGSIGRSYSGDFQRWQLFPGRCE 439
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig99743.22924.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A0G4E8B5_VITBC5.450e-2146.32Uncharacterized protein n=2 Tax=Vitrella brassicaf... [more]
Q54D71_DICDI2.580e-2048.39Uncharacterized protein n=1 Tax=Dictyostelium disc... [more]
A0A8J4PXL4_9MYCE6.540e-2048.39Uncharacterized protein n=1 Tax=Polysphondylium vi... [more]
A0A8J5WEF8_ZIZPA8.670e-1944.79Glyco_hydr_116N domain-containing protein n=1 Tax=... [more]
A0A7J7CGI8_TRIWF1.060e-1842.39Non-lysosomal glucosylceramidase n=2 Tax=Tripteryg... [more]
F1A2R6_DICPU2.730e-1843.01Uncharacterized protein n=1 Tax=Dictyostelium purp... [more]
UPI000CED50223.690e-1847.87non-lysosomal glucosylceramidase n=1 Tax=Morus not... [more]
A0A151Z2R2_9MYCE5.080e-1844.09Uncharacterized protein n=1 Tax=Tieghemostelium la... [more]
UPI0005114AE16.830e-1846.81non-lysosomal glucosylceramidase-like isoform X1 n... [more]
A0A498JEH2_MALDO6.970e-1845.74BUB1 N-terminal domain-containing protein n=1 Tax=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig99743contigM-pyrifera_M_contig99743:88..402 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score96.3
Seed ortholog evalue9.1e-18
Seed eggNOG ortholog218851.Aquca_017_00087.1
KEGG rclassRC00059,RC00451
KEGG koko:K17108
KEGG ReactionR01498
KEGG Pathwayko00511,ko00600,ko01100,map00511,map00600,map01100
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004348,GO:0004553,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005790,GO:0005886,GO:0006082,GO:0006629,GO:0006643,GO:0006664,GO:0006665,GO:0006687,GO:0006807,GO:0007275,GO:0007399,GO:0007417,GO:0008150,GO:0008152,GO:0008202,GO:0008206,GO:0008422,GO:0009056,GO:0009987,GO:0012505,GO:0015926,GO:0016020,GO:0016021,GO:0016137,GO:0016139,GO:0016787,GO:0016798,GO:0019752,GO:0021953,GO:0021954,GO:0022008,GO:0030154,GO:0030182,GO:0031224,GO:0032501,GO:0032502,GO:0032787,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0044237,GO:0044238,GO:0044255,GO:0044281,GO:0044424,GO:0044425,GO:0044444,GO:0044464,GO:0048468,GO:0048666,GO:0048699,GO:0048731,GO:0048856,GO:0048869,GO:0071704,GO:0071944,GO:1901135,GO:1901136,GO:1901360,GO:1901564,GO:1901575,GO:1901615,GO:1901657,GO:1901658,GO:1903509
EggNOG free text desc.Non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide to free glucose and ceramide
EggNOG OGs37JKE@33090,3GG94@35493,COG4354@1,KOG2119@2759
EC3.2.1.45
COG Functional cat.G
CAZyGH116
Best tax levelStreptophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons1
Model size315
Cds size315
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816914.2449536-CDS-M-pyrifera_M_contig99743:87..4021622816914.2449536-CDS-M-pyrifera_M_contig99743:87..402Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99743 88..402 -
1692278252.5589263-CDS-M-pyrifera_M_contig99743:87..4021692278252.5589263-CDS-M-pyrifera_M_contig99743:87..402Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig99743 88..402 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig99743.22924.1prot_M-pyrifera_M_contig99743.22924.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig99743 88..402 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig99743.22924.1

>prot_M-pyrifera_M_contig99743.22924.1 ID=prot_M-pyrifera_M_contig99743.22924.1|Name=mRNA_M-pyrifera_M_contig99743.22924.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=105bp
MAAARAQGIPSAAWQKNLEDEDVEHESFSVGIKEGIKLAGLGLRMSKFIR
KETREGREPFMDPFGSYDVHSNAGVPCGGIGAGSIGRGYKGDFNSFLLRP
GRPE*
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mRNA from alignment at M-pyrifera_M_contig99743:88..402-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig99743.22924.1 ID=mRNA_M-pyrifera_M_contig99743.22924.1|Name=mRNA_M-pyrifera_M_contig99743.22924.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=315bp|location=Sequence derived from alignment at M-pyrifera_M_contig99743:88..402- (Macrocystis pyrifera P11B4 male)
ATGGCCGCTGCGAGGGCCCAAGGAATACCCTCGGCTGCTTGGCAGAAGAA
TTTGGAGGACGAGGACGTGGAGCATGAGTCGTTCTCTGTGGGCATTAAGG
AGGGCATCAAGCTCGCGGGCCTGGGCCTGCGAATGAGCAAGTTCATCCGC
AAGGAGACCAGGGAGGGCCGCGAGCCGTTCATGGACCCGTTTGGGAGCTA
CGATGTGCACTCCAACGCAGGCGTGCCGTGCGGAGGCATCGGCGCCGGCT
CGATCGGCCGCGGATACAAGGGCGACTTCAACTCCTTCCTCCTGCGGCCT
GGGAGGCCAGAGTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig99743:88..402-

>mRNA_M-pyrifera_M_contig99743.22924.1 ID=mRNA_M-pyrifera_M_contig99743.22924.1|Name=mRNA_M-pyrifera_M_contig99743.22924.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=630bp|location=Sequence derived from alignment at M-pyrifera_M_contig99743:88..402- (Macrocystis pyrifera P11B4 male)
ATGGCCGCTGCGAGGGCCCAAGGAATACCCTCGGCTGCTTGGCAGAAGAA
TTTGGAGGACGAGGACGTGGAGCATGAGTCGTTCTCTGTGGGCATTAAGG
AGGGCATCAAGCTCGCGGGCCTGGGCCTGCGAATGAGCAAGTTCATCCGC
AAGGAGACCAGGGAGGGCCGCGAGCCGTTCATGGACCCGTTTGGGAGCTA
CGATGTGCACTCCAACGCAGGCGTGCCGTGCGGAGGCATCGGCGCCGGCT
CGATCGGCCGCGGATACAAGGGCGACTTCAACTCCTTCCTCCTGCGGCCT
GGGAGGCCAGAGTAGATGGCCGCTGCGAGGGCCCAAGGAATACCCTCGGC
TGCTTGGCAGAAGAATTTGGAGGACGAGGACGTGGAGCATGAGTCGTTCT
CTGTGGGCATTAAGGAGGGCATCAAGCTCGCGGGCCTGGGCCTGCGAATG
AGCAAGTTCATCCGCAAGGAGACCAGGGAGGGCCGCGAGCCGTTCATGGA
CCCGTTTGGGAGCTACGATGTGCACTCCAACGCAGGCGTGCCGTGCGGAG
GCATCGGCGCCGGCTCGATCGGCCGCGGATACAAGGGCGACTTCAACTCC
TTCCTCCTGCGGCCTGGGAGGCCAGAGTAG
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