mRNA_M-pyrifera_M_contig98968.22765.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig98968.22765.1
Unique NamemRNA_M-pyrifera_M_contig98968.22765.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig98968.22765.1 vs. uniprot
Match: A0A7S4M8J9_9EUKA (Hypothetical protein n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4M8J9_9EUKA)

HSP 1 Score: 172 bits (435), Expect = 1.110e-46
Identity = 88/163 (53.99%), Postives = 119/163 (73.01%), Query Frame = 1
Query:   16 DVVQRLRALNNFSTMMAFIAGLNSAAVSRLKWTKREVVK--MHTLEELERQMSSELNYKSYRTLLAAASGTPCIPYLGVHLSDLTFIEEGNPNMLEGTRLINFERRHQICDVIQAVTLPQKRLFNLQPVSNIIALLTDLPTMTEKHLYQLSLTREPRGGPPPD 498
            ++ + L+ ++N++++MAFIA LN+AAVSRLKWTK+E+ K  + TL+ELE+ MS+E+NYK YR  +++ +   C+PYLGVHLSDL FIEEGN   LE + L+NFE   Q+CDVI  +   Q   +  + V  ++ LLTDLPTM EK LYQLSL REPRG  PPD
Sbjct:  665 NIAKHLKTMHNYNSVMAFIAALNTAAVSRLKWTKKELSKRSLDTLQELEKFMSNEMNYKQYRNNVSSVTDA-CVPYLGVHLSDLIFIEEGNSKYLENSNLVNFEMCQQVCDVISLLKKLQGVSYTAKSVPELMVLLTDLPTMNEKPLYQLSLKREPRGKIPPD 826          
BLAST of mRNA_M-pyrifera_M_contig98968.22765.1 vs. uniprot
Match: UPI000644F540 (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644F540)

HSP 1 Score: 132 bits (332), Expect = 1.170e-32
Identity = 73/161 (45.34%), Postives = 108/161 (67.08%), Query Frame = 1
Query:    4 ENEADVVQRLRALNNFSTMMAFIAGLNSAAVSRLKWTKREVVK--MHTLEELERQMSSELNYKSYRTLLAAASGTPCIPYLGVHLSDLTFIEEGNPNMLEGTRLINFERRHQICDVIQAVTLPQKRLFNLQPVSNIIALLTDLPTMTEKHLYQLSLTREPR 480
            E    + ++LR + N++T+MAF+ GLN++A+ RLK+TK  V +  +  LE+LE++MS E +YK+YR LL      PC+PY+GV+L+DLTFIEEGNPN++ G  LINF +   +  VI  V   Q   +NL PV+ I + +++L     + LYQLSL +EPR
Sbjct: 1248 ERVIKIAEKLREMKNYNTLMAFLGGLNNSAILRLKYTKAIVGRKYLEALEDLEKEMSCESSYKTYRELLHNTD-PPCVPYIGVYLTDLTFIEEGNPNII-GNNLINFSKYTLMYKVISEVQQYQWSEYNLHPVNIIQSFISNLQPPNVEALYQLSLLKEPR 1406          
BLAST of mRNA_M-pyrifera_M_contig98968.22765.1 vs. uniprot
Match: A0A8J4PPZ1_9MYCE (Uncharacterized protein n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4PPZ1_9MYCE)

HSP 1 Score: 132 bits (331), Expect = 1.460e-32
Identity = 76/153 (49.67%), Postives = 101/153 (66.01%), Query Frame = 1
Query:   31 LRALNNFSTMMAFIAGLNSAAVSRLKWTKREVVKMHTLE---ELERQMSSELNYKSYRTLLAAASGTPCIPYLGVHLSDLTFIEEGNPNMLEGTRLINFERRHQICDVIQAVTLPQKRLFNLQPVSNIIALLTDLPTMTEKHLYQLSLTREPR 480
            +R+LNNF+T MA ++GLN+A+V RLK+TK E+ K HT +   EL+ Q+SS   YK YR +L+  S  PC+PYLGV+L+DLTF EEGNP+ ++G   INF +R  I + I  V   Q   +NLQPV  I  LL +LP   E  LY  S+  EPR
Sbjct:  646 MRSLNNFNTSMAILSGLNAASVHRLKFTKEEMPK-HTQQVYTELQAQLSSNSAYKEYRAILSK-SNPPCLPYLGVYLTDLTFFEEGNPDYIQG--FINFSKRKLIYNSISNVQSFQNTKYNLQPVYQIATLLKNLPRSDEDKLYHKSMAYEPR 794          
BLAST of mRNA_M-pyrifera_M_contig98968.22765.1 vs. uniprot
Match: F0ZEF7_DICPU (Uncharacterized protein n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F0ZEF7_DICPU)

HSP 1 Score: 131 bits (329), Expect = 2.460e-32
Identity = 76/153 (49.67%), Postives = 102/153 (66.67%), Query Frame = 1
Query:   31 LRALNNFSTMMAFIAGLNSAAVSRLKWTKREVVKMHTLE---ELERQMSSELNYKSYRTLLAAASGTPCIPYLGVHLSDLTFIEEGNPNMLEGTRLINFERRHQICDVIQAVTLPQKRLFNLQPVSNIIALLTDLPTMTEKHLYQLSLTREPR 480
            ++ LNNF+T MA ++GLN+++V RLK+TK E+ K HT +   EL++Q+SS  +YK YR LLA A+  PC+PYLGV+L+DLTF EEGNP+ ++G   INF +R  I   I  V   Q   +NLQPV  I  LL D    TE  LY+ S+  EPR
Sbjct:  563 MKLLNNFNTSMAILSGLNASSVHRLKFTKEEMPK-HTQQVYAELQQQLSSSQSYKEYRALLAKAN-PPCLPYLGVYLTDLTFFEEGNPDFIQG--YINFGKRKLIYGSISNVQSFQNAKYNLQPVYQITKLLKDFKLSTENELYKQSMKVEPR 711          
BLAST of mRNA_M-pyrifera_M_contig98968.22765.1 vs. uniprot
Match: F1A0X6_DICPU (Uncharacterized protein (Fragment) n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F1A0X6_DICPU)

HSP 1 Score: 131 bits (329), Expect = 2.630e-32
Identity = 72/152 (47.37%), Postives = 100/152 (65.79%), Query Frame = 1
Query:   31 LRALNNFSTMMAFIAGLNSAAVSRLKWTKREVVK--MHTLEELERQMSSELNYKSYRTLLAAASGTPCIPYLGVHLSDLTFIEEGNPNMLEGTRLINFERRHQICDVIQAVTLPQKRLFNLQPVSNIIALLTDLPTMTEKHLYQLSLTREPR 480
            +++LNNF+T MA ++GLN++++ RLK+TK E+ K  M   ++L++Q+SS   YK YR LLA  S  PC+PYLGV+L+DLTF E+GNP+ ++G   INF +R  I   I  V   Q   +NLQPV  I  LLT    + E  LY  S+T EPR
Sbjct:  605 MKSLNNFNTSMAILSGLNASSIHRLKFTKEEMPKHTMQVYQDLQQQLSSAQAYKEYRALLAK-SNPPCLPYLGVYLTDLTFFEDGNPDFIQG--FINFGKRKLIYGSISNVQSFQNTKYNLQPVYQIAKLLTGFKLLNENELYSRSMTYEPR 753          
BLAST of mRNA_M-pyrifera_M_contig98968.22765.1 vs. uniprot
Match: L8HHY3_ACACA (RasGEF domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8HHY3_ACACA)

HSP 1 Score: 130 bits (328), Expect = 4.130e-32
Identity = 72/158 (45.57%), Postives = 105/158 (66.46%), Query Frame = 1
Query:   16 DVVQRLRALNNFSTMMAFIAGLNSAAVSRLKWTKREVVK--MHTLEELERQMSSELNYKSYRTLLAAASGTPCIPYLGVHLSDLTFIEEGNPNMLEGTRLINFERRHQICDVIQAVTLPQKRLFNLQPVSNIIALLTDLPTMTEKHLYQLSLTREPRG 483
            ++ + L ALNNFS++MAFIAG N+++V RLKWT +++ K  +   + LE  MS + ++++YRT +  A   PC+PY+GV+L DL FIEEGNP+ L    LINF +R  +   I+ +   Q++ +NL  V  I AL  +LPT ++  LY LSL REPRG
Sbjct: 1716 NIAKCLYALNNFSSLMAFIAGWNTSSVIRLKWTMKDLPKKTIELRDMLEGVMSCDFSWRAYRTAIHEAK-PPCLPYIGVYLQDLIFIEEGNPDNLGD--LINFSKRELVASAIRELQQYQQQAYNLLEVPRISALFVNLPTKSDDELYNLSLKREPRG 1870          
BLAST of mRNA_M-pyrifera_M_contig98968.22765.1 vs. uniprot
Match: A0A507BLV9_9FUNG (Uncharacterized protein n=1 Tax=Synchytrium microbalum TaxID=1806994 RepID=A0A507BLV9_9FUNG)

HSP 1 Score: 129 bits (325), Expect = 9.940e-32
Identity = 77/164 (46.95%), Postives = 105/164 (64.02%), Query Frame = 1
Query:   16 DVVQRLRALNNFSTMMAFIAGLNSAAVSRLKWTKREVVKMHT--LEELERQMSSELNYKSYRTLLAAASGTPCIPYLGVHLSDLTFIEEGNPNMLEGTRLINFERRHQICDVIQAVTLPQKRLFNLQPVSNIIALLTDLPTMT---EKHLYQLSLTREPRGGPP 492
            ++  +L ALNNFST+MAFIAGLN AA+ RLK+T +E+       L +LE+ M++E +YK+YR  L   +  PCIPY+GV+L DLT++E+GNPNM++   LINF +R  I  VI+ +   Q    N + V  +  LL   P  T   EK LY+LSL REPR   P
Sbjct:  856 EIATQLHALNNFSTLMAFIAGLNKAAIMRLKYTFKELSSSSRKKLADLEKLMTAENSYKAYRAALHTVN-PPCIPYIGVYLIDLTYMEDGNPNMVDN--LINFTKRTMISTVIRELQQYQSSPVNFRIVDELARLLNAFPDATDTFEKLLYELSLKREPRDQTP 1016          
BLAST of mRNA_M-pyrifera_M_contig98968.22765.1 vs. uniprot
Match: L8H8A0_ACACA (RasGEF domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8H8A0_ACACA)

HSP 1 Score: 129 bits (324), Expect = 1.340e-31
Identity = 75/161 (46.58%), Postives = 100/161 (62.11%), Query Frame = 1
Query:    4 ENEADVVQRLRALNNFSTMMAFIAGLNSAAVSRLKWTKREVVKM--HTLEELERQMSSELNYKSYRTLLAAASGTPCIPYLGVHLSDLTFIEEGNPNMLEGTRLINFERRHQICDVIQAVTLPQKRLFNLQPVSNIIALLTDLPTMTEKHLYQLSLTREPR 480
            E  A + + L  L NF+T+MA +AG+ S  V RL +T+ EV +    TLE L   M  +  YK+YR +L+  S  PCIPYLGVHLSDLTFIE+GNP+ + G  LINF +R  +  VI  ++  Q+  +NL PV  I +LLT+     +  LY LSL REPR
Sbjct:  778 ETFASIAKHLYNLKNFNTLMALLAGIRSGPVYRLAYTRAEVSRKADKTLEHLNLLMRGDNAYKTYRDVLSQ-SAPPCIPYLGVHLSDLTFIEDGNPDTIRG--LINFTKRRFLFRVISEISRYQQNAYNLHPVPQIQSLLTNFTMKKDDELYDLSLQREPR 935          
BLAST of mRNA_M-pyrifera_M_contig98968.22765.1 vs. uniprot
Match: A0A152A2Z1_9MYCE (Ras guanine nucleotide exchange factor n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A152A2Z1_9MYCE)

HSP 1 Score: 127 bits (319), Expect = 5.930e-31
Identity = 72/152 (47.37%), Postives = 99/152 (65.13%), Query Frame = 1
Query:   34 RALNNFSTMMAFIAGLNSAAVSRLKWTKREVVK--MHTLEELERQMSSELNYKSYRTLLAAASGTPCIPYLGVHLSDLTFIEEGNPNMLEGTRLINFERRHQICDVIQAVTLPQKRLFNLQPVSNIIALLTDL-PTMTEKHLYQLSLTREPR 480
            RALNNF+  MA ++GLN+A+V RLK+TK ++ K    TL++L+ Q S+ ++Y+ YR  LA A+  PCIPYLGVHL+DLTF+EEGNP++++G   INF +R  +   I  +   Q   +NLQPV  I  LL  L P   E   Y  S+  EPR
Sbjct:  609 RALNNFNVSMAILSGLNAASVHRLKFTKDDMPKHTQQTLQDLQNQFSNAMSYRDYRNQLAKAN-PPCIPYLGVHLTDLTFVEEGNPDLIQGQ--INFSKRRLVYQSISIIHNLQNTKYNLQPVYQIAKLLRTLKPRFDEDEQYHRSMKFEPR 757          
BLAST of mRNA_M-pyrifera_M_contig98968.22765.1 vs. uniprot
Match: F4PXZ9_CAVFA (Ras guanine nucleotide exchange factor n=1 Tax=Cavenderia fasciculata (strain SH3) TaxID=1054147 RepID=F4PXZ9_CAVFA)

HSP 1 Score: 127 bits (318), Expect = 9.140e-31
Identity = 73/157 (46.50%), Postives = 102/157 (64.97%), Query Frame = 1
Query:   19 VVQRLRALNNFSTMMAFIAGLNSAAVSRLKWTKREVVKMHT--LEELERQMSSELNYKSYRTLLAAASGTPCIPYLGVHLSDLTFIEEGNPNMLEGTRLINFERRHQICDVIQAVTLPQKRLFNLQPVSNIIALLTDLPTMTEKHLYQLSLTREPRG 483
            + ++LR L N++T+M+F+ GLN++A+ RLK+T+  V K +T  LE LE++MS E +YKSYR +L      PCIP++GV+L DLTFIEEGNPN+L    LINF +   +  VI  V   Q   +NL  V  I   + +L    EK LY +SL +EPRG
Sbjct: 1409 IAEKLRELKNYNTLMSFLGGLNNSALLRLKFTRALVSKRYTETLEGLEKEMSCEGSYKSYRGILHNTD-PPCIPFVGVYLQDLTFIEEGNPNILPEHSLINFSKYTLMYKVISEVQTYQWSDYNLNIVPIIQTFIRELNPPPEKDLYSISLQKEPRG 1564          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig98968.22765.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S4M8J9_9EUKA1.110e-4653.99Hypothetical protein n=1 Tax=Vannella sp. CB-2014 ... [more]
UPI000644F5401.170e-3245.34hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
A0A8J4PPZ1_9MYCE1.460e-3249.67Uncharacterized protein n=1 Tax=Polysphondylium vi... [more]
F0ZEF7_DICPU2.460e-3249.67Uncharacterized protein n=1 Tax=Dictyostelium purp... [more]
F1A0X6_DICPU2.630e-3247.37Uncharacterized protein (Fragment) n=1 Tax=Dictyos... [more]
L8HHY3_ACACA4.130e-3245.57RasGEF domain containing protein n=1 Tax=Acanthamo... [more]
A0A507BLV9_9FUNG9.940e-3246.95Uncharacterized protein n=1 Tax=Synchytrium microb... [more]
L8H8A0_ACACA1.340e-3146.58RasGEF domain containing protein n=1 Tax=Acanthamo... [more]
A0A152A2Z1_9MYCE5.930e-3147.37Ras guanine nucleotide exchange factor n=1 Tax=Tie... [more]
F4PXZ9_CAVFA9.140e-3146.50Ras guanine nucleotide exchange factor n=1 Tax=Cav... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig98968contigM-pyrifera_M_contig98968:106..603 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score131.0
Seed ortholog evalue5.3e-28
Seed eggNOG ortholog5786.XP_003285807.1
KEGG koko:K03099,ko:K06277
KEGG Pathwayko01521,ko01522,ko04010,ko04012,ko04013,ko04014,ko04015,ko04062,ko04068,ko04072,ko04150,ko04151,ko04320,ko04510,ko04540,ko04630,ko04650,ko04660,ko04662,ko04664,ko04714,ko04722,ko04810,ko04910,ko04912,ko04915,ko04917,ko04926,ko05034,ko05160,ko05165,ko05200,ko05205,ko05206,ko05210,ko05211,ko05213,ko05214,ko05215,ko05220,ko05221,ko05223,ko05224,ko05225,ko05226,ko05231,map01521,map01522,map04010,map04012,map04013,map04014,map04015,map04062,map04068,map04072,map04150,map04151,map04320,map04510,map04540,map04630,map04650,map04660,map04662,map04664,map04714,map04722,map04810,map04910,map04912,map04915,map04917,map04926,map05034,map05160,map05165,map05200,map05205,map05206,map05210,map05211,map05213,map05214,map05215,map05220,map05221,map05223,map05224,map05225,map05226,map05231
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Ras guanine nucleotide exchange factor
EggNOG OGs3XFBB@554915,KOG3417@1,KOG3417@2759
COG Functional cat.T
Best tax levelAmoebozoa
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001
Exons1
Model size498
Cds size498
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816906.3674655-CDS-M-pyrifera_M_contig98968:105..6031622816906.3674655-CDS-M-pyrifera_M_contig98968:105..603Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig98968 106..603 +
1692278248.0062547-CDS-M-pyrifera_M_contig98968:105..6031692278248.0062547-CDS-M-pyrifera_M_contig98968:105..603Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig98968 106..603 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig98968.22765.1prot_M-pyrifera_M_contig98968.22765.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig98968 106..603 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig98968.22765.1

>prot_M-pyrifera_M_contig98968.22765.1 ID=prot_M-pyrifera_M_contig98968.22765.1|Name=mRNA_M-pyrifera_M_contig98968.22765.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=166bp
QENEADVVQRLRALNNFSTMMAFIAGLNSAAVSRLKWTKREVVKMHTLEE
LERQMSSELNYKSYRTLLAAASGTPCIPYLGVHLSDLTFIEEGNPNMLEG
TRLINFERRHQICDVIQAVTLPQKRLFNLQPVSNIIALLTDLPTMTEKHL
YQLSLTREPRGGPPPD
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mRNA from alignment at M-pyrifera_M_contig98968:106..603+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig98968.22765.1 ID=mRNA_M-pyrifera_M_contig98968.22765.1|Name=mRNA_M-pyrifera_M_contig98968.22765.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=498bp|location=Sequence derived from alignment at M-pyrifera_M_contig98968:106..603+ (Macrocystis pyrifera P11B4 male)
CAGGAGAATGAGGCTGACGTGGTGCAGCGGCTGCGTGCGCTGAACAACTT CAGCACGATGATGGCGTTCATCGCGGGCCTGAATTCGGCAGCGGTGTCGC GGCTGAAGTGGACGAAGCGGGAGGTGGTGAAGATGCACACGCTGGAGGAG CTGGAGCGGCAGATGAGCAGCGAGCTGAACTACAAGAGCTACAGAACGCT GCTGGCCGCGGCCTCGGGCACGCCTTGCATTCCCTACCTGGGCGTCCACC TCTCAGACCTCACCTTCATCGAGGAGGGCAACCCCAACATGCTTGAGGGC ACGCGGCTGATCAACTTCGAGCGGCGCCACCAGATCTGCGACGTGATCCA GGCCGTGACGCTGCCCCAGAAGCGCCTCTTCAACCTCCAGCCCGTCAGCA ACATCATCGCCCTGCTCACCGACCTGCCCACCATGACGGAGAAGCATCTC TACCAGCTCTCCCTCACCCGCGAGCCCCGCGGCGGCCCTCCCCCCGAC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig98968:106..603+

>mRNA_M-pyrifera_M_contig98968.22765.1 ID=mRNA_M-pyrifera_M_contig98968.22765.1|Name=mRNA_M-pyrifera_M_contig98968.22765.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=996bp|location=Sequence derived from alignment at M-pyrifera_M_contig98968:106..603+ (Macrocystis pyrifera P11B4 male)
CAGGAGAATGAGGCTGACGTGGTGCAGCGGCTGCGTGCGCTGAACAACTT
CAGCACGATGATGGCGTTCATCGCGGGCCTGAATTCGGCAGCGGTGTCGC
GGCTGAAGTGGACGAAGCGGGAGGTGGTGAAGATGCACACGCTGGAGGAG
CTGGAGCGGCAGATGAGCAGCGAGCTGAACTACAAGAGCTACAGAACGCT
GCTGGCCGCGGCCTCGGGCACGCCTTGCATTCCCTACCTGGGCGTCCACC
TCTCAGACCTCACCTTCATCGAGGAGGGCAACCCCAACATGCTTGAGGGC
ACGCGGCTGATCAACTTCGAGCGGCGCCACCAGATCTGCGACGTGATCCA
GGCCGTGACGCTGCCCCAGAAGCGCCTCTTCAACCTCCAGCCCGTCAGCA
ACATCATCGCCCTGCTCACCGACCTGCCCACCATGACGGAGAAGCATCTC
TACCAGCTCTCCCTCACCCGCGAGCCCCGCGGCGGCCCTCCCCCCGACCA
GGAGAATGAGGCTGACGTGGTGCAGCGGCTGCGTGCGCTGAACAACTTCA
GCACGATGATGGCGTTCATCGCGGGCCTGAATTCGGCAGCGGTGTCGCGG
CTGAAGTGGACGAAGCGGGAGGTGGTGAAGATGCACACGCTGGAGGAGCT
GGAGCGGCAGATGAGCAGCGAGCTGAACTACAAGAGCTACAGAACGCTGC
TGGCCGCGGCCTCGGGCACGCCTTGCATTCCCTACCTGGGCGTCCACCTC
TCAGACCTCACCTTCATCGAGGAGGGCAACCCCAACATGCTTGAGGGCAC
GCGGCTGATCAACTTCGAGCGGCGCCACCAGATCTGCGACGTGATCCAGG
CCGTGACGCTGCCCCAGAAGCGCCTCTTCAACCTCCAGCCCGTCAGCAAC
ATCATCGCCCTGCTCACCGACCTGCCCACCATGACGGAGAAGCATCTCTA
CCAGCTCTCCCTCACCCGCGAGCCCCGCGGCGGCCCTCCCCCCGAC
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