mRNA_M-pyrifera_M_contig9871.22722.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig9871.22722.1
Unique NamemRNA_M-pyrifera_M_contig9871.22722.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig9871.22722.1 vs. uniprot
Match: D8LHX5_ECTSI (Predicted hydrolases or acyltransferases (Alpha/beta hydrolase superfamily) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LHX5_ECTSI)

HSP 1 Score: 181 bits (458), Expect = 3.190e-53
Identity = 86/94 (91.49%), Postives = 90/94 (95.74%), Query Frame = 1
Query:   22 QVLTQVYPDPRNVDDELVESIRVPSLDPNAPEVFQRVITRTGGPGITVDDLLDGFKSPLLLLWGELDPWIRPAAADKIQALYPAAKRFSVQAGH 303
            QVLTQVYPDPRNVDDELVESIRVPSLDPNAPEVFQRVI+RTGGPG+TVD+LL+GF SPLLLLWGELDPWIRPAAADKIQ LYP A RFSVQAGH
Sbjct:  321 QVLTQVYPDPRNVDDELVESIRVPSLDPNAPEVFQRVISRTGGPGLTVDELLEGFTSPLLLLWGELDPWIRPAAADKIQGLYPEATRFSVQAGH 414          
BLAST of mRNA_M-pyrifera_M_contig9871.22722.1 vs. uniprot
Match: A0A7S2EXP3_TRICV (Hypothetical protein n=1 Tax=Trieres chinensis TaxID=1514140 RepID=A0A7S2EXP3_TRICV)

HSP 1 Score: 127 bits (318), Expect = 4.200e-35
Identity = 68/96 (70.83%), Postives = 72/96 (75.00%), Query Frame = 1
Query:   22 QVLTQVYP-DPRNVDDELVESIRVPSLDPNAPEVFQRVITRTG-GPGITVDDLLDGFKSPLLLLWGELDPWIRPAAADKIQALYPAAKRFSVQAGH 303
            QVL QVYP +   VD ELVESIRVPS DPNA EVF RVIT+ G GP   VDDLL     PLLL WGE DPWIRPAAADKIQALYP +KR S+ AGH
Sbjct:   55 QVLRQVYPINADMVDPELVESIRVPSTDPNAAEVFYRVITKNGSGPQTYVDDLLQTLDCPLLLAWGESDPWIRPAAADKIQALYPRSKRVSIDAGH 150          
BLAST of mRNA_M-pyrifera_M_contig9871.22722.1 vs. uniprot
Match: A0A7S2HZ54_9STRA (Hypothetical protein n=1 Tax=Helicotheca tamesis TaxID=374047 RepID=A0A7S2HZ54_9STRA)

HSP 1 Score: 124 bits (312), Expect = 6.420e-35
Identity = 64/92 (69.57%), Postives = 72/92 (78.26%), Query Frame = 1
Query:   34 QVYP-DPRNVDDELVESIRVPSLDPNAPEVFQRVITRTGG-PGITVDDLLDGFKSPLLLLWGELDPWIRPAAADKIQALYPAAKRFSVQAGH 303
            QVYP +  NVD+ELVESIR PSLDPNA EVF RVI R+ G P + VDDLL+  + PLLL WGE DPWIRP AADKIQALYP +KR S+ AGH
Sbjct:    1 QVYPVNDDNVDEELVESIRAPSLDPNAAEVFYRVIDRSSGTPKVFVDDLLEELECPLLLCWGEEDPWIRPQAADKIQALYPESKRVSIDAGH 92          
BLAST of mRNA_M-pyrifera_M_contig9871.22722.1 vs. uniprot
Match: A0A7S1ZKC8_9STRA (Hypothetical protein n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A7S1ZKC8_9STRA)

HSP 1 Score: 127 bits (318), Expect = 3.160e-33
Identity = 67/97 (69.07%), Postives = 75/97 (77.32%), Query Frame = 1
Query:   22 QVLTQVYP-DPRNVDDELVESIRVPSLDPNAPEVFQRVITRTG-GPGITVDDLLDGFKS-PLLLLWGELDPWIRPAAADKIQALYPAAKRFSVQAGH 303
            QVL QVYP +  NVD+ELVESIR PSLDPNA EVF RVIT+ G GP + VDDLL      PL+L WGE DPWIRPAAADKIQALYP++ R S+ AGH
Sbjct:  235 QVLRQVYPINANNVDEELVESIRYPSLDPNAAEVFYRVITKNGSGPQVYVDDLLQDLNGIPLMLCWGENDPWIRPAAADKIQALYPSSLRVSIDAGH 331          
BLAST of mRNA_M-pyrifera_M_contig9871.22722.1 vs. uniprot
Match: A0A1E7FKN7_9STRA (Alpha/beta-hydrolase n=1 Tax=Fragilariopsis cylindrus CCMP1102 TaxID=635003 RepID=A0A1E7FKN7_9STRA)

HSP 1 Score: 121 bits (303), Expect = 2.280e-31
Identity = 64/96 (66.67%), Postives = 71/96 (73.96%), Query Frame = 1
Query:   22 QVLTQVYP-DPRNVDDELVESIRVPSLDPNAPEVFQRVITR-TGGPGITVDDLLDGFKSPLLLLWGELDPWIRPAAADKIQALYPAAKRFSVQAGH 303
            QVL QVYP D  NV+D+LVESIR PS DPNA EVF RVIT+ T G  + VDD+L+    PLLL WGELDPWIRP  ADKIQ LYP A R S+ AGH
Sbjct:  182 QVLNQVYPVDNSNVNDKLVESIRFPSNDPNAAEVFFRVITKNTNGSAVYVDDVLEKLDCPLLLCWGELDPWIRPETADKIQKLYPRAIRASINAGH 277          
BLAST of mRNA_M-pyrifera_M_contig9871.22722.1 vs. uniprot
Match: A0A6U1NRJ5_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A6U1NRJ5_9STRA)

HSP 1 Score: 119 bits (299), Expect = 5.410e-30
Identity = 60/95 (63.16%), Postives = 71/95 (74.74%), Query Frame = 1
Query:   22 QVLTQVYPDPRNVDDELVESIRVPSLDPNAPEVFQRVITRTGG-PGITVDDLLDGFKSPLLLLWGELDPWIRPAAADKIQALYPAAKRFSVQAGH 303
            QVL+QVY D +NVDD+LVESIR P+ +PNAPEVF RVI R GG P  +V+ LL     PLLLLWGE DPWIRPAAA KI  +YP+A+   + AGH
Sbjct:  307 QVLSQVYTDTKNVDDDLVESIRFPAENPNAPEVFYRVIRRNGGGPQTSVNTLLQQLSVPLLLLWGEQDPWIRPAAAQKIMEIYPSAEMVPLVAGH 401          
BLAST of mRNA_M-pyrifera_M_contig9871.22722.1 vs. uniprot
Match: A0A7S2UMU3_9STRA (Hypothetical protein n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2UMU3_9STRA)

HSP 1 Score: 115 bits (289), Expect = 7.990e-29
Identity = 61/97 (62.89%), Postives = 71/97 (73.20%), Query Frame = 1
Query:   22 QVLTQVYPDPRN-VDDELVESIRVPSLDPNAPEVFQRVITR--TGGPGITVDDLLDGFKSPLLLLWGELDPWIRPAAADKIQALYPAAKRFSVQAGH 303
            QVL QVYP   N VD ELV+SI++PS DPNA EVF RVI +  +G P + VDDLL   + PLLL WGE DPWIRPAAADKIQ LY  ++R S+ AGH
Sbjct:  251 QVLRQVYPINANMVDQELVDSIQLPSQDPNAAEVFYRVIKKNSSGRPSVYVDDLLQRLECPLLLCWGEADPWIRPAAADKIQTLYTNSQRVSIPAGH 347          
BLAST of mRNA_M-pyrifera_M_contig9871.22722.1 vs. uniprot
Match: A0A7S2PTK7_9STRA (Hypothetical protein n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A7S2PTK7_9STRA)

HSP 1 Score: 107 bits (266), Expect = 2.090e-25
Identity = 58/110 (52.73%), Postives = 71/110 (64.55%), Query Frame = 1
Query:   22 QVLTQVYP-DPRNVDDELVESIRVPSLDPNAPEVFQRVITRTG-GPGITVDDLLDGFKSPLLLLWGELDPWIRPAAADKIQALYPA--------------AKRFSVQAGH 303
            Q+L  VYP D  NV+ ELVESIRVP+LDPNA EVF RVIT+ G GP   +DD++   + P+LL WGE DPWIRPAAADK+Q L+                 KR ++ AGH
Sbjct:  259 QILKSVYPSDSSNVNSELVESIRVPALDPNAAEVFYRVITKNGSGPQAFIDDIIKELECPVLLAWGEKDPWIRPAAADKMQMLHAEYHGKNVGGESGSKWIKRVNIDAGH 368          
BLAST of mRNA_M-pyrifera_M_contig9871.22722.1 vs. uniprot
Match: A0A7S4G4X5_9EUGL (Hypothetical protein n=1 Tax=Eutreptiella gymnastica TaxID=73025 RepID=A0A7S4G4X5_9EUGL)

HSP 1 Score: 101 bits (251), Expect = 4.860e-25
Identity = 56/99 (56.57%), Postives = 63/99 (63.64%), Query Frame = 1
Query:   22 QVLTQVYPDPRNVDDELVESIRVPSLDPNAPEVFQRVITRTG--GPGITVD---DLLDGFKSPLLLLWGELDPWIRPAAADKIQALYPAAKRFSVQAGH 303
            QVL QVY D  NVDDELV SI   +  P APEVF  VITR     P  T+D    LL     P+LLLWGE DPWIRP A ++IQ LYP A+R S+ AGH
Sbjct:   45 QVLNQVYIDRTNVDDELVNSIEYAAQAPTAPEVFYSVITRNSVATPQSTIDFLLALLSKADHPMLLLWGEQDPWIRPQAGNRIQRLYPRAERVSINAGH 143          
BLAST of mRNA_M-pyrifera_M_contig9871.22722.1 vs. uniprot
Match: B5YNN1_THAPS (Predicted protein n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B5YNN1_THAPS)

HSP 1 Score: 102 bits (255), Expect = 3.060e-24
Identity = 56/101 (55.45%), Postives = 68/101 (67.33%), Query Frame = 1
Query:    7 PGINVQVLTQVYP-DPRNVDDELVESIRVPSLDPNAPEVFQRVITRTG-GPGITVDDLLDGFKSPLLLLWGELDPWIRPAAADKIQALYPAAKRFSVQAGH 303
            P    Q+L  VYP +  NVD ELVESI+ P+LDPNA EVF RVIT+ G GP   VDD+L   + P+LL WGE DPWI+ AAADK++ L+      SV AGH
Sbjct:  199 PARITQILKNVYPINNANVDSELVESIQTPALDPNAAEVFYRVITKNGSGPQAYVDDILKEMECPVLLAWGESDPWIKSAAADKMERLHA---EISVDAGH 296          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig9871.22722.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LHX5_ECTSI3.190e-5391.49Predicted hydrolases or acyltransferases (Alpha/be... [more]
A0A7S2EXP3_TRICV4.200e-3570.83Hypothetical protein n=1 Tax=Trieres chinensis Tax... [more]
A0A7S2HZ54_9STRA6.420e-3569.57Hypothetical protein n=1 Tax=Helicotheca tamesis T... [more]
A0A7S1ZKC8_9STRA3.160e-3369.07Hypothetical protein n=1 Tax=Ditylum brightwellii ... [more]
A0A1E7FKN7_9STRA2.280e-3166.67Alpha/beta-hydrolase n=1 Tax=Fragilariopsis cylind... [more]
A0A6U1NRJ5_9STRA5.410e-3063.16Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A7S2UMU3_9STRA7.990e-2962.89Hypothetical protein n=1 Tax=Attheya septentrional... [more]
A0A7S2PTK7_9STRA2.090e-2552.73Hypothetical protein n=1 Tax=Skeletonema marinoi T... [more]
A0A7S4G4X5_9EUGL4.860e-2556.57Hypothetical protein n=1 Tax=Eutreptiella gymnasti... [more]
B5YNN1_THAPS3.060e-2455.45Predicted protein n=1 Tax=Thalassiosira pseudonana... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig9871contigM-pyrifera_M_contig9871:6268..8995 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score181.4
Seed ortholog evalue2.1e-43
Seed eggNOG ortholog2880.D8LHX5
Hectar predicted targeting categoryother localisation
EggNOG free text desc.regulation of endocannabinoid signaling pathway
EggNOG OGsCOG0596@1,KOG1454@2759
Ec32 ortholog descriptionEpoxide hydrolase-like
Ec32 orthologEc-18_000850.1
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons3
Model size309
Cds size309
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig9871.22722.1prot_M-pyrifera_M_contig9871.22722.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig9871 6268..8995 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816904.370861-CDS-M-pyrifera_M_contig9871:6267..63181622816904.370861-CDS-M-pyrifera_M_contig9871:6267..6318Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9871 6268..6318 -
1692278246.8761828-CDS-M-pyrifera_M_contig9871:6267..63181692278246.8761828-CDS-M-pyrifera_M_contig9871:6267..6318Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9871 6268..6318 -
1622816904.3829377-CDS-M-pyrifera_M_contig9871:7671..78121622816904.3829377-CDS-M-pyrifera_M_contig9871:7671..7812Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9871 7672..7812 -
1692278246.8899567-CDS-M-pyrifera_M_contig9871:7671..78121692278246.8899567-CDS-M-pyrifera_M_contig9871:7671..7812Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9871 7672..7812 -
1622816904.3943844-CDS-M-pyrifera_M_contig9871:8878..89951622816904.3943844-CDS-M-pyrifera_M_contig9871:8878..8995Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9871 8879..8995 -
1692278246.9001503-CDS-M-pyrifera_M_contig9871:8878..89951692278246.9001503-CDS-M-pyrifera_M_contig9871:8878..8995Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig9871 8879..8995 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig9871.22722.1

>prot_M-pyrifera_M_contig9871.22722.1 ID=prot_M-pyrifera_M_contig9871.22722.1|Name=mRNA_M-pyrifera_M_contig9871.22722.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=103bp
MIPGINVQVLTQVYPDPRNVDDELVESIRVPSLDPNAPEVFQRVITRTGG
PGITVDDLLDGFKSPLLLLWGELDPWIRPAAADKIQALYPAAKRFSVQAG
HW*
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mRNA from alignment at M-pyrifera_M_contig9871:6268..8995-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig9871.22722.1 ID=mRNA_M-pyrifera_M_contig9871.22722.1|Name=mRNA_M-pyrifera_M_contig9871.22722.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=2728bp|location=Sequence derived from alignment at M-pyrifera_M_contig9871:6268..8995- (Macrocystis pyrifera P11B4 male)
ATGATACCTGGTATCAATGTACAGGTTCTTACGCAGGTGTACCCAGACCC TCGAAATGTGGACGACGAGTTGGTGGAGAGCATCCGTGTTCCTTCCTTGG ACCCCAACGCTCCCGAGGTCCAGTACGAATATTTTTACATTGGTTTGGCC TTGCCTTGCCTCGCCTTGCCTTGCCTTGCCTTGCCTTACCTTGCCATCGC TGTAGTTTTTTGTGCGCCGAATAACCCTGCATCCTAAGACGCGCAAAAAA AACAACAAATGATTTCGAAGGGTATTCGCTCGTGTTCGCTTTTATGCGTT TGTTGTTTTTTATGTTTTCGTCTTTGCTTATTTTCGCTTGGCTTGTCTTT GGCTTTGCTCTTCTTTTCTTGCCTCTTCGTCGTGTTCTCATGTATTCTTG GCTACATTTTTTGCTTTGTTTTTCTCCTTCTTTTTGCACCAGATATTCTC CGCATCCCAACACGCCTAAAGGTTTCCAACGCGACATTTCGAGGATTCTT CGTTTTTCTTTTTTGCTTCACATTACCTTGGCTAATTTTGGCTTTGCTCT TCTCGTTTGTGCAGTCTTTCGTCTCTCTTCATTTTTTTTCTTTGCTTTTT GTTTTGCTTTGTTTTTTTCTTTGCTTCACACCGGATGACCCCCCGTATCC CCACAATTCCCATTCCTATCCCCCCCCCCACTCCTACCGTCTCGAAGGGA TGGGTGGTCAATGGTCAAATGACTGTGGCAAACAACAAAGGATTAACTCC CCGCCCCTCCAGCGGTATACCGTCGTTCCCCCATCCTCGCCCCCGCCCCC CACAATGGCCAATTACTAAGGCAAGCAACAAATGAGCACGCCTCTCCGCC CCCTCCGAGGCATTTACATAATATACCCCTTGCTGTCTCTGGCAGTGACC ACACCAGAAAAAAAATATAAGAATTTGGCGCCGTCATCCGTAAGCAAGAA AATCCGCCTCACAATCGGTTCTTGGTAGGATTCAGCTCACCTTTCATGCC ACTCGCCGAACACCTGGCGGCGCTTTGTGCTTGGTGCAAGCAATGTCCGC CTGGTCGGTGAAAATCCGACGTGCTCCGATCCGACATTTTAGAGATGCCA GGGTGTATAGTCATATGACTACGGCCATGCATGACATGGGTACCCCTCCC TCCCCTGCCATTTTCCATCATTTCCTGCGCAAGGTTTTCCAACGCGTCAT CACTAGGACAGGCGGACCGGGGATAACCGTAGACGACCTCCTTGACGGGT TCAAGTCCCCTTTGCTGCTTCTTTGGGGGGAACTTGACCCTTGGATCCGA CCTGCCGCCGCGGATAAAATTCAGGCGAGATGAATGCCGTGGTGGTGATA TTTTAAGGCCATCCGGTTTGAAGCCATAGTAGAGAAAATACCCAATAAAT CGGTTACTGTATTCACGAGAAAAACTACTTGGAATTAGTGCGCAATAATT GTTTCATTAGTAAATGATAAGAACATTCTAGAGATAAGACCATGTTTTTT GGGCATGTGAAGGGATGAGTCTTGAAAATGGGTCCACCACTCCTGTACCA TTACATGGCTATTCGTGTACCAGGTATATTATTTTGCTGCGGGAAGGTCG CAGCTTTTTTTCTGAGGCTTCTCTCTGAAATAACTCCATATCTCCGAAAC CTTACCCGGAGAAAGAGCGTATACTTACCACAACCCCCATGATAAAGTAT GTAACAATCTTTGTAAACAGGAACGGGATTGAAACATTATTGGGGCGAAG AGCCTGGTCAGTCAGAAACGTATTGGCAAGGCACGGGGCGTGGGTGATGT TCAGGGGATATAATCAGGTCCATACAAATAAGGATCGTTTCGGTATGCGT GTTAAGAACCCAACATCCCTTACATTTTCGGACATAGTAGTATCGCGCGT CGAATACATGGGCAGTATATAGTCTAGGCAAGGCACGCGCACAACGTCTG TACCTGCGGTAGGCAGCCTGAAGAGTGTAGCCGTTGTGTACCCGTCGTAT ATCCAAACGGTCGTGTTCCGTTGTGTATTTGTCGTATAACCGTCGTGTAG CGGTCGTGTACCCGTCGTGAAACGGTCGTTGATCCGTCGTATAGCCGTCG TGTAATGGTCGTGTAGTCGTCATACAACCGTCGTGTAACCGTATTTTAAC CGTGATGTAGCCGTCGTGTAACCGTCGTGTGAACGTCGTAAATCCGTCGT GTAATCGTCGCCCAACAGTCGTAAAACCTCTTTGTTGTCGTCGTGTAACC ATCGTGTAATCGTCGTGTGACCGTCGCGAAGCTGACACCTGCGGTTAGTA TGACAGTCGCGTTGACCGAACTAGCTACAGCTAGCCAGCAGCAACGCAGT ATATCTCACAGCAGTATGATGGGTACTGAGTAATACTGACAGTATACGGG CATTGGTGGATACTGCTGTGAGCTCCTCTGCAAAAATAGCAGGCGAGAGA CGATACTTCTCCTGGGGGCACGAGAGTTTATTTGCACAGACTTGTGTTTT GTTTCACCTCTGTACCTTTTTGTCGGGTTGAAGCGGTGGTTCCTTTGCTA TGCTGCCCCCACTCCCCCCTCCTACACTGCCTTCCTTCGCCTACATACCC TTCGTTGTCACCTGTATCGCCTTGATCTTCGTCGACCGTGTTTTTAATTG TGGTTTGTTTTTGTTTTCGGCTTTCAGGCGCTGTACCCGGCTGCGAAACG GTTCAGCGTCCAGGCCGGACACTGGTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig9871:6268..8995-

>mRNA_M-pyrifera_M_contig9871.22722.1 ID=mRNA_M-pyrifera_M_contig9871.22722.1|Name=mRNA_M-pyrifera_M_contig9871.22722.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=618bp|location=Sequence derived from alignment at M-pyrifera_M_contig9871:6268..8995- (Macrocystis pyrifera P11B4 male)
ATGATACCTGGTATCAATGTACAGGTTCTTACGCAGGTGTACCCAGACCC
TCGAAATGTGGACGACGAGTTGGTGGAGAGCATCCGTGTTCCTTCCTTGG
ACCCCAACGCTCCCGAGATGATACCTGGTATCAATGTACAGGTTCTTACG
CAGGTGTACCCAGACCCTCGAAATGTGGACGACGAGTTGGTGGAGAGCAT
CCGTGTTCCTTCCTTGGACCCCAACGCTCCCGAGGTTTTCCAACGCGTCA
TCACTAGGACAGGCGGACCGGGGATAACCGTAGACGACCTCCTTGACGGG
TTCAAGTCCCCTTTGCTGCTTCTTTGGGGGGAACTTGACCCTTGGATCCG
ACCTGCCGCCGCGGATAAAATTCAGGTTTTCCAACGCGTCATCACTAGGA
CAGGCGGACCGGGGATAACCGTAGACGACCTCCTTGACGGGTTCAAGTCC
CCTTTGCTGCTTCTTTGGGGGGAACTTGACCCTTGGATCCGACCTGCCGC
CGCGGATAAAATTCAGGCGCTGTACCCGGCTGCGAAACGGTTCAGCGTCC
AGGCCGGACACTGGTGAGCGCTGTACCCGGCTGCGAAACGGTTCAGCGTC
CAGGCCGGACACTGGTGA
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