mRNA_M-pyrifera_M_contig97342.22422.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig97342.22422.1
Unique NamemRNA_M-pyrifera_M_contig97342.22422.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig97342.22422.1 vs. uniprot
Match: A0A813BR97_9DINO (Ankrd13b protein (Fragment) n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813BR97_9DINO)

HSP 1 Score: 181 bits (458), Expect = 5.060e-53
Identity = 85/161 (52.80%), Postives = 113/161 (70.19%), Query Frame = 1
Query:    1 LRTAVMAMLQETDAAWQRRVPIIQDRLGKLPDFSMELTWEFSSWIPLVGTLLPRDTMKIRKRGSSLRIEYTLLGMEGFSWGRGDLAFVIRGDSDP---PGAVFVLDNEEKTAGDARLAFTDPQDYQVQDWVRKTLTKEQKVSDWWSRSVEFVRVHECGWLG 474
            +R+ VMAML+ETD A+QRR P + +    +PDF M + WEFSSWIP V  LLP+DTM IRKRG++LR++ TLLGM G +W RGD++ ++R  ++     G   +LDN+ +TAGD R +F  P+D  VQDWVRK L+  QKVSDWWS+   F +V   GWLG
Sbjct:  118 VRSTVMAMLRETDIAFQRRAPRLAEGFASMPDFEMTMVWEFSSWIPFVSGLLPKDTMTIRKRGTALRMDSTLLGMSGMTWQRGDVSQIVRVQAERGSHEGQFLLLDNKARTAGDGRESFLCPEDKNVQDWVRKVLSTPQKVSDWWSKDTTFKQVFTKGWLG 278          
BLAST of mRNA_M-pyrifera_M_contig97342.22422.1 vs. uniprot
Match: A0A5A8DR08_CAFRO (Uncharacterized protein n=3 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DR08_CAFRO)

HSP 1 Score: 170 bits (430), Expect = 7.850e-47
Identity = 76/158 (48.10%), Postives = 111/158 (70.25%), Query Frame = 1
Query:    4 RTAVMAMLQETDAAWQRRVPIIQDRLGKLPDFSMELTWEFSSWIPLVGTLLPRDTMKIRKRGSSLRIEYTLLGMEGFSWGRGDLAFVIRGDSDP-PGAVFVLDNEEKTAGDARLAFTDPQDYQVQDWVRKTLTKEQKVSDWWSRSVEFVRVHECGWLG 474
            R AV++ML++ D AW+RR+P   DRL  +PDF M + W+FSSW+PLV T+LPRDT+ + KRG+++R++ TLLG  G SWGRG ++F+++G+    PGA FV+DNE KTA +AR A T+P D+ ++DWVRK L  +QK + W   ++         W+G
Sbjct:  105 RIAVVSMLKDMDLAWERRLPKHLDRLRAMPDFVMTMNWDFSSWVPLVSTILPRDTVTVYKRGTAVRVDTTLLGFSGMSWGRGSISFIVQGEEQGNPGAWFVMDNELKTAANARAALTNPPDHTIEDWVRKLLGNQQKRTKWNGAALRLETEKRSTWMG 262          
BLAST of mRNA_M-pyrifera_M_contig97342.22422.1 vs. uniprot
Match: UPI000359E77C (ankyrin repeat domain-containing protein 13C isoform X1 n=2 Tax=Aplysia californica TaxID=6500 RepID=UPI000359E77C)

HSP 1 Score: 101 bits (252), Expect = 2.450e-22
Identity = 51/151 (33.77%), Postives = 87/151 (57.62%), Query Frame = 1
Query:   28 QETDAAWQRRVPIIQDRLGKLPDFSMELTWEFSSWIPLVGTLLPRDTMKIRKRGSSLRIEYTLLGMEGFSWGRGDLAFVIRGDSDPPGAVFVLDNEEKTAGDARLAFTDPQDYQVQDWVRKTLTKEQKVSDWWSRSVEFVRVHECGWLGEE 480
            Q++  A ++R P+I   L  + DF MEL W+F SW+PLV ++LP D  +IRK+GS +R++ TL+      W RGD+ F+  G+++P  ++ VLDN+ K     R   TD    ++++ V   ++ +   +   ++ + F R  + GWL  E
Sbjct:   94 QQSREALEQRRPLIVQALKDIGDFDMELKWDFQSWVPLVSSILPSDICRIRKKGSRIRLDTTLVDFTEMRWERGDITFMFNGEAEPRNSLTVLDNKAKVFQRVRYEDTD---IELEEEVDILMSSDIVAAQMSTKKITFSRA-QSGWLFRE 240          
BLAST of mRNA_M-pyrifera_M_contig97342.22422.1 vs. uniprot
Match: UPI0006B0F2AA (ankyrin repeat domain-containing protein 13C-like isoform X1 n=3 Tax=Limulus polyphemus TaxID=6850 RepID=UPI0006B0F2AA)

HSP 1 Score: 101 bits (252), Expect = 2.460e-22
Identity = 51/148 (34.46%), Postives = 86/148 (58.11%), Query Frame = 1
Query:   28 QETDAAWQRRVPIIQDRLGKLPDFSMELTWEFSSWIPLVGTLLPRDTMKIRKRGSSLRIEYTLLGMEGFSWGRGDLAFVIRGDSDPPGAVFVLDNEEKTAGDARLAFTDPQDYQVQDWVRKTLTKEQKVSDWWSRSVEFVRVHECGWL 471
            Q++  A + R P +   L ++ DF MEL W+F SW+PLV  LLP D  +IRK+GSS+R++ TL+      W RGD++F+  G++ P  ++ VLDNE+K     R   T+ +   ++D +   ++ +       ++S+ F R  + GW+
Sbjct:   96 QQSREAMETRRPDLVRALNQMGDFYMELKWDFQSWVPLVSRLLPSDICRIRKKGSSIRLDTTLVDFNDMRWERGDVSFIFSGNAKPSQSLTVLDNEQKVYQKVRYEETEAE---IEDEIDLLMSSDIVSVQMSTKSITFSRA-QTGWI 239          
BLAST of mRNA_M-pyrifera_M_contig97342.22422.1 vs. uniprot
Match: UPI000719CE4E (ankyrin repeat domain-containing protein 13C-like n=1 Tax=Priapulus caudatus TaxID=37621 RepID=UPI000719CE4E)

HSP 1 Score: 99.4 bits (246), Expect = 1.220e-21
Identity = 52/151 (34.44%), Postives = 85/151 (56.29%), Query Frame = 1
Query:   28 QETDAAWQRRVPIIQDRLGKLPDFSMELTWEFSSWIPLVGTLLPRDTMKIRKRGSSLRIEYTLLGMEGFSWGRGDLAFVIRGDSDPPGAVFVLDNEEKTAGDARLAFTDPQDYQVQDWVRKTLTKEQKVSDWWSRSVEFVRVHECGWLGEE 480
            Q++  A + R P + D L +L DF MEL W+F SW+PLV  +LP D  +I KRGSS+R++ TLL      W RGD++F+  GD+ P  ++ VLDN   +    R    +  + ++++ V   ++ +   +   ++S+ F R  + GW   E
Sbjct:   50 QQSREALEERRPKLTDALKQLGDFYMELKWDFQSWVPLVSRILPSDICRIHKRGSSIRMDTTLLDFNDMRWERGDISFIFDGDAKPSHSLTVLDNRVNSYQKIRY---EESEVEIEEEVDILMSSDIVAAQMSTKSITFSRS-QSGWFFRE 196          
BLAST of mRNA_M-pyrifera_M_contig97342.22422.1 vs. uniprot
Match: A0A2J7QMX1_9NEOP (Ankyrin repeat domain-containing protein 13C-A n=2 Tax=Cryptotermes secundus TaxID=105785 RepID=A0A2J7QMX1_9NEOP)

HSP 1 Score: 99.0 bits (245), Expect = 1.620e-21
Identity = 51/160 (31.87%), Postives = 87/160 (54.37%), Query Frame = 1
Query:    1 LRTAVMAMLQETDAAWQRRVPIIQDRLGKLPDFSMELTWEFSSWIPLVGTLLPRDTMKIRKRGSSLRIEYTLLGMEGFSWGRGDLAFVIRGDSDPPGAVFVLDNEEKTAGDARLAFTDPQDYQVQDWVRKTLTKEQKVSDWWSRSVEFVRVHECGWLGEE 480
            + + V  + Q+     + R P +   L ++ DF MEL W+F SW+PLV  +LP D  +I KRGSS+R++ TL+      W RGD++F+  GD  P  ++ VLDN+ +     R   T+    +++D V   ++ +   +   ++S+ F R  + GW+  E
Sbjct:   41 ISSLVRKLKQQAREQMEERRPNLVSALNQMGDFYMELKWDFQSWVPLVSRILPSDICRIHKRGSSIRLDTTLVDFNDMRWERGDISFLFNGDRKPNLSLTVLDNKAELFQRVRYEETE---VEIEDEVDILMSSDIMAAQMSTKSITFTRA-QTGWIFRE 196          
BLAST of mRNA_M-pyrifera_M_contig97342.22422.1 vs. uniprot
Match: A0A7R9J3Z1_TIMCA ((California timema) hypothetical protein n=1 Tax=Timema californicum TaxID=61474 RepID=A0A7R9J3Z1_TIMCA)

HSP 1 Score: 98.2 bits (243), Expect = 2.140e-21
Identity = 52/160 (32.50%), Postives = 86/160 (53.75%), Query Frame = 1
Query:    1 LRTAVMAMLQETDAAWQRRVPIIQDRLGKLPDFSMELTWEFSSWIPLVGTLLPRDTMKIRKRGSSLRIEYTLLGMEGFSWGRGDLAFVIRGDSDPPGAVFVLDNEEKTAGDARLAFTDPQDYQVQDWVRKTLTKEQKVSDWWSRSVEFVRVHECGWLGEE 480
            L + V  + Q+     + R P +   L ++ DF MEL W+F SW+PLV  +LP D   I KRGSS+R++ TL+      W RGD++F+  GD  P  ++ VLDN+ +     R   T+ +   ++D V   ++ +   +   ++S+ F R  + GW+  E
Sbjct:    3 LLSLVRKLKQQAREQMEERRPNLVAALNQMGDFYMELKWDFQSWVPLVSRILPSDICHIHKRGSSIRLDTTLVDFNDMHWERGDISFLFTGDRKPSQSLTVLDNKAQLFQRVRYEETETE---IEDEVDILMSSDIMAAQMSTKSITFTRA-QTGWIFRE 158          
BLAST of mRNA_M-pyrifera_M_contig97342.22422.1 vs. uniprot
Match: A0A067R3V7_ZOONE (Ankyrin repeat domain-containing protein 13C n=7 Tax=Polyneoptera TaxID=33341 RepID=A0A067R3V7_ZOONE)

HSP 1 Score: 99.0 bits (245), Expect = 2.370e-21
Identity = 51/160 (31.87%), Postives = 87/160 (54.37%), Query Frame = 1
Query:    1 LRTAVMAMLQETDAAWQRRVPIIQDRLGKLPDFSMELTWEFSSWIPLVGTLLPRDTMKIRKRGSSLRIEYTLLGMEGFSWGRGDLAFVIRGDSDPPGAVFVLDNEEKTAGDARLAFTDPQDYQVQDWVRKTLTKEQKVSDWWSRSVEFVRVHECGWLGEE 480
            + + V  + Q+     + R P +   L ++ DF MEL W+F SW+PLV  +LP D  +I KRGSS+R++ TL+      W RGD++F+  GD  P  ++ VLDN+ +     R   T+    +++D V   ++ +   +   ++S+ F R  + GW+  E
Sbjct:   87 ISSLVRKLKQQAREQMEERRPNLVSALNQMGDFYMELKWDFQSWVPLVSRILPSDICRIHKRGSSIRLDTTLVDFNDMRWERGDISFLFNGDRKPNLSLTVLDNKAELYQRVRYEETE---MEIEDEVDILMSSDIMAAQMSTKSITFTRA-QTGWIFRE 242          
BLAST of mRNA_M-pyrifera_M_contig97342.22422.1 vs. uniprot
Match: A0A6A4VZI4_AMPAM (Ankyrin repeat domain-containing protein 13C n=2 Tax=Amphibalanus amphitrite TaxID=1232801 RepID=A0A6A4VZI4_AMPAM)

HSP 1 Score: 99.0 bits (245), Expect = 2.740e-21
Identity = 48/140 (34.29%), Postives = 83/140 (59.29%), Query Frame = 1
Query:   52 RRVPIIQDRLGKLPDFSMELTWEFSSWIPLVGTLLPRDTMKIRKRGSSLRIEYTLLGMEGFSWGRGDLAFVIRGDSDPPGAVFVLDNEEKTAGDARLAFTDPQDYQVQDWVRKTLTKEQKVSDWWSRSVEFVRVHECGWL 471
            RR P +   L ++PDF ME+ W+F +W+PL+  +LP DT KI KRG+S+R++ TL+    F W RGD++F+  GDS P  ++ VLDN+ +     R   T+    ++++ +   ++ +   +   ++S+ F R  + GW 
Sbjct:  124 RRRPALVAALHQIPDFYMEIKWDFQTWVPLLHRILPSDTCKIYKRGASIRLDTTLVDFADFKWERGDISFLFNGDSPPSQSLSVLDNKLRVYQTVRYEETE---LEIEEEIDILMSSDIVSAQMSTKSITFSRAQQ-GWF 259          
BLAST of mRNA_M-pyrifera_M_contig97342.22422.1 vs. uniprot
Match: A0A8D8X0P7_9HEMI (Ankyrin repeat domain-containing protein 13C n=2 Tax=Cacopsylla melanoneura TaxID=428564 RepID=A0A8D8X0P7_9HEMI)

HSP 1 Score: 98.6 bits (244), Expect = 3.640e-21
Identity = 52/152 (34.21%), Postives = 86/152 (56.58%), Query Frame = 1
Query:   25 LQETDAAWQRRVPIIQDRLGKLPDFSMELTWEFSSWIPLVGTLLPRDTMKIRKRGSSLRIEYTLLGMEGFSWGRGDLAFVIRGDSDPPGAVFVLDNEEKTAGDARLAFTDPQDYQVQDWVRKTLTKEQKVSDWWSRSVEFVRVHECGWLGEE 480
            LQ      +RR  ++Q  L ++ DF MEL W+F SW+PLV  +LP D  KI K+GSS+R++ TL+      W RGD++F+  GD +P  ++ VLDN  +   + R   T+    +++D V   ++ +   +   ++S+ F R  + GW+  E
Sbjct:  112 LQARQQMEERRPNLVQ-ALTQINDFYMELKWDFQSWVPLVSRILPSDICKIYKKGSSIRLDTTLVDFNDMRWERGDISFLFNGDGNPSESLVVLDNLSQLFQNVRYEETE---LEIEDEVDILMSSDIMAAQMSTKSISFARA-QSGWIFRE 258          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig97342.22422.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A813BR97_9DINO5.060e-5352.80Ankrd13b protein (Fragment) n=1 Tax=Symbiodinium s... [more]
A0A5A8DR08_CAFRO7.850e-4748.10Uncharacterized protein n=3 Tax=Cafeteria roenberg... [more]
UPI000359E77C2.450e-2233.77ankyrin repeat domain-containing protein 13C isofo... [more]
UPI0006B0F2AA2.460e-2234.46ankyrin repeat domain-containing protein 13C-like ... [more]
UPI000719CE4E1.220e-2134.44ankyrin repeat domain-containing protein 13C-like ... [more]
A0A2J7QMX1_9NEOP1.620e-2131.88Ankyrin repeat domain-containing protein 13C-A n=2... [more]
A0A7R9J3Z1_TIMCA2.140e-2132.50(California timema) hypothetical protein n=1 Tax=T... [more]
A0A067R3V7_ZOONE2.370e-2131.88Ankyrin repeat domain-containing protein 13C n=7 T... [more]
A0A6A4VZI4_AMPAM2.740e-2134.29Ankyrin repeat domain-containing protein 13C n=2 T... [more]
A0A8D8X0P7_9HEMI3.640e-2134.21Ankyrin repeat domain-containing protein 13C n=2 T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig97342contigM-pyrifera_M_contig97342:222..701 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score100.5
Seed ortholog evalue7.2e-19
Seed eggNOG ortholog6500.XP_005110837.1
Preferred nameANKRD13C
KEGG koko:K21437
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0005102,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0006621,GO:0008104,GO:0008150,GO:0009889,GO:0009987,GO:0010556,GO:0010869,GO:0010941,GO:0012505,GO:0019222,GO:0031323,GO:0032507,GO:0033036,GO:0033365,GO:0034613,GO:0035437,GO:0042981,GO:0043067,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044424,GO:0044444,GO:0044464,GO:0045185,GO:0048471,GO:0050789,GO:0050794,GO:0051179,GO:0051235,GO:0051641,GO:0051651,GO:0060255,GO:0065007,GO:0065008,GO:0070727,GO:0070972,GO:0072595,GO:2000209
EggNOG free text desc.protein retention in ER lumen
EggNOG OGs38CG8@33154,3BCNI@33208,3CTWV@33213,KOG0522@1,KOG0522@2759
COG Functional cat.S
Best tax levelBilateria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko04131
Exons1
Model size480
Cds size465
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816890.2423441-CDS-M-pyrifera_M_contig97342:221..6861622816890.2423441-CDS-M-pyrifera_M_contig97342:221..686Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig97342 222..686 -
1692278238.915156-CDS-M-pyrifera_M_contig97342:221..6861692278238.915156-CDS-M-pyrifera_M_contig97342:221..686Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig97342 222..686 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816890.257522-UTR-M-pyrifera_M_contig97342:686..7011622816890.257522-UTR-M-pyrifera_M_contig97342:686..701Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig97342 687..701 -
1692278238.9393-UTR-M-pyrifera_M_contig97342:686..7011692278238.9393-UTR-M-pyrifera_M_contig97342:686..701Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig97342 687..701 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig97342.22422.1prot_M-pyrifera_M_contig97342.22422.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig97342 222..686 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig97342.22422.1

>prot_M-pyrifera_M_contig97342.22422.1 ID=prot_M-pyrifera_M_contig97342.22422.1|Name=mRNA_M-pyrifera_M_contig97342.22422.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=155bp
MAMLQETDAAWQRRVPIIQDRLGKLPDFSMELTWEFSSWIPLVGTLLPRD
TMKIRKRGSSLRIEYTLLGMEGFSWGRGDLAFVIRGDSDPPGAVFVLDNE
EKTAGDARLAFTDPQDYQVQDWVRKTLTKEQKVSDWWSRSVEFVRVHECG
WLGEE
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mRNA from alignment at M-pyrifera_M_contig97342:222..701-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig97342.22422.1 ID=mRNA_M-pyrifera_M_contig97342.22422.1|Name=mRNA_M-pyrifera_M_contig97342.22422.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=480bp|location=Sequence derived from alignment at M-pyrifera_M_contig97342:222..701- (Macrocystis pyrifera P11B4 male)
TTGCGTACAGCTGTGATGGCTATGTTACAAGAGACTGATGCTGCATGGCA AAGACGTGTTCCAATTATCCAAGATCGACTGGGAAAGCTGCCAGATTTCT CTATGGAGCTAACGTGGGAGTTTTCATCATGGATTCCACTAGTTGGTACG TTATTACCACGTGATACGATGAAAATTCGGAAGCGTGGGTCTTCTTTGAG GATTGAATATACTCTATTGGGCATGGAAGGGTTCAGTTGGGGGCGTGGAG ACTTAGCCTTTGTCATTCGGGGGGATAGTGATCCACCTGGTGCTGTCTTT GTATTGGACAATGAGGAAAAAACTGCTGGAGATGCAAGGTTGGCATTTAC AGATCCTCAAGACTACCAAGTACAAGATTGGGTGCGAAAAACGTTGACCA AGGAACAAAAAGTCTCTGATTGGTGGTCCCGCTCGGTGGAATTTGTTCGT GTACATGAGTGTGGATGGTTGGGTGAGGAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig97342:222..701-

>mRNA_M-pyrifera_M_contig97342.22422.1 ID=mRNA_M-pyrifera_M_contig97342.22422.1|Name=mRNA_M-pyrifera_M_contig97342.22422.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=930bp|location=Sequence derived from alignment at M-pyrifera_M_contig97342:222..701- (Macrocystis pyrifera P11B4 male)
ATGGCTATGTTACAAGAGACTGATGCTGCATGGCAAAGACGTGTTCCAAT
TATCCAAGATCGACTGGGAAAGCTGCCAGATTTCTCTATGGAGCTAACGT
GGGAGTTTTCATCATGGATTCCACTAGTTGGTACGTTATTACCACGTGAT
ACGATGAAAATTCGGAAGCGTGGGTCTTCTTTGAGGATTGAATATACTCT
ATTGGGCATGGAAGGGTTCAGTTGGGGGCGTGGAGACTTAGCCTTTGTCA
TTCGGGGGGATAGTGATCCACCTGGTGCTGTCTTTGTATTGGACAATGAG
GAAAAAACTGCTGGAGATGCAAGGTTGGCATTTACAGATCCTCAAGACTA
CCAAGTACAAGATTGGGTGCGAAAAACGTTGACCAAGGAACAAAAAGTCT
CTGATTGGTGGTCCCGCTCGGTGGAATTTGTTCGTGTACATGAGTGTGGA
TGGTTGGGTGAGGAAATGGCTATGTTACAAGAGACTGATGCTGCATGGCA
AAGACGTGTTCCAATTATCCAAGATCGACTGGGAAAGCTGCCAGATTTCT
CTATGGAGCTAACGTGGGAGTTTTCATCATGGATTCCACTAGTTGGTACG
TTATTACCACGTGATACGATGAAAATTCGGAAGCGTGGGTCTTCTTTGAG
GATTGAATATACTCTATTGGGCATGGAAGGGTTCAGTTGGGGGCGTGGAG
ACTTAGCCTTTGTCATTCGGGGGGATAGTGATCCACCTGGTGCTGTCTTT
GTATTGGACAATGAGGAAAAAACTGCTGGAGATGCAAGGTTGGCATTTAC
AGATCCTCAAGACTACCAAGTACAAGATTGGGTGCGAAAAACGTTGACCA
AGGAACAAAAAGTCTCTGATTGGTGGTCCCGCTCGGTGGAATTTGTTCGT
GTACATGAGTGTGGATGGTTGGGTGAGGAA
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