mRNA_M-pyrifera_M_contig97330.22419.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig97330.22419.1
Unique NamemRNA_M-pyrifera_M_contig97330.22419.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig97330.22419.1 vs. uniprot
Match: A0A2E7KEH3_9PLAN (Uncharacterized protein n=6 Tax=Gimesia TaxID=1649453 RepID=A0A2E7KEH3_9PLAN)

HSP 1 Score: 456 bits (1174), Expect = 4.820e-157
Identity = 235/236 (99.58%), Postives = 236/236 (100.00%), Query Frame = 1
Query:    1 QLEEMTGWSSMIFQTVRTMRDEQEPERMQGEWIDFRPADALPNVLFKQIKVSGNARVNQQKYPFQALVRELSSEPTAYRRPIQLQARVEAEGEVRLAGELKFYEQQPTHDFLVKFDLPKQKTIQLENSDKLSLALIADQTECESRISFRENDFTCRVEFKQTPVRFQFDSEQAGIKPLTQVVAQALSTIDSISATLEGSGSYSQPVWRLESETGEQIARGLKLACQAEIGRQKQAL 708
            QLEEMTGWSSMIFQTVRTMRDEQEPERMQGEWIDFRPADALPNVLFKQIKVSGNARVNQQKYPFQALVRELSSEPTAYRRPIQLQARVEAEGEVRLAGELKFYEQQPTHDFLVKFDLPKQKTIQLENSDKLSLALIADQTECESRISFR+NDFTCRVEFKQTPVRFQFDSEQAGIKPLTQVVAQALSTIDSISATLEGSGSYSQPVWRLESETGEQIARGLKLACQAEIGRQKQAL
Sbjct:  286 QLEEMTGWSSMIFQTVRTMRDEQEPERMQGEWIDFRPADALPNVLFKQIKVSGNARVNQQKYPFQALVRELSSEPTAYRRPIQLQARVEAEGEVRLAGELKFYEQQPTHDFLVKFDLPKQKTIQLENSDKLSLALIADQTECESRISFRQNDFTCRVEFKQTPVRFQFDSEQAGIKPLTQVVAQALSTIDSISATLEGSGSYSQPVWRLESETGEQIARGLKLACQAEIGRQKQAL 521          
BLAST of mRNA_M-pyrifera_M_contig97330.22419.1 vs. uniprot
Match: A0A518A9U4_9PLAN (Uncharacterized protein n=3 Tax=Gimesia panareensis TaxID=2527978 RepID=A0A518A9U4_9PLAN)

HSP 1 Score: 375 bits (962), Expect = 5.570e-125
Identity = 186/236 (78.81%), Postives = 217/236 (91.95%), Query Frame = 1
Query:    1 QLEEMTGWSSMIFQTVRTMRDEQEPERMQGEWIDFRPADALPNVLFKQIKVSGNARVNQQKYPFQALVRELSSEPTAYRRPIQLQARVEAEGEVRLAGELKFYEQQPTHDFLVKFDLPKQKTIQLENSDKLSLALIADQTECESRISFRENDFTCRVEFKQTPVRFQFDSEQAGIKPLTQVVAQALSTIDSISATLEGSGSYSQPVWRLESETGEQIARGLKLACQAEIGRQKQAL 708
            Q+E++ GWS++IFQTV+TMRDE EPERMQGEWIDF+PA ALP+VLFKQI +SG ARVNQQKYPFQALVRELSS PT Y+RPI LQAR++AEGEV+LAG+LKFY  +PTHDF+VKF LP+QKTIQLE+SDKLSLALIADQTEC S+IS RE DF CRVEF+QTPVRF+F S Q+GIKPL QVV++ALS+IDS+SATLEGSGSYSQPVW++ESETG+QIA+G+KLACQAEIGRQKQ L
Sbjct:  286 QVEQVFGWSNVIFQTVKTMRDEPEPERMQGEWIDFQPAGALPDVLFKQIHLSGTARVNQQKYPFQALVRELSSAPTEYQRPIHLQARIDAEGEVKLAGDLKFYLARPTHDFVVKFQLPRQKTIQLEDSDKLSLALIADQTECVSQISLREKDFACRVEFRQTPVRFEFASSQSGIKPLAQVVSRALSSIDSVSATLEGSGSYSQPVWKIESETGQQIAQGMKLACQAEIGRQKQEL 521          
BLAST of mRNA_M-pyrifera_M_contig97330.22419.1 vs. uniprot
Match: A0A518IFZ0_9PLAN (Uncharacterized protein n=2 Tax=Gimesia TaxID=1649453 RepID=A0A518IFZ0_9PLAN)

HSP 1 Score: 292 bits (747), Expect = 1.220e-92
Identity = 142/236 (60.17%), Postives = 190/236 (80.51%), Query Frame = 1
Query:    1 QLEEMTGWSSMIFQTVRTMRDEQEPERMQGEWIDFRPADALPNVLFKQIKVSGNARVNQQKYPFQALVRELSSEPTAYRRPIQLQARVEAEGEVRLAGELKFYEQQPTHDFLVKFDLPKQKTIQLENSDKLSLALIADQTECESRISFRENDFTCRVEFKQTPVRFQFDSEQAGIKPLTQVVAQALSTIDSISATLEGSGSYSQPVWRLESETGEQIARGLKLACQAEIGRQKQAL 708
            QLE+++GWS M+F+TV+ +RDEQEPERMQGEWIDFR    LP +LFK+I++SGNARV+QQKYPF  L++ELSS P+ YR+PIQLQA+VEAE E++ AG+LKFYE  PTH+F+V F  P QK I++E+SDKLS+ALIADQTEC+S ++ RE+DF CR+EF Q+PVRF+  +   G + +  +V Q L++ID I+ATL  SGSY +P  RLESE GE++A+ LKLA Q+++ +QKQ L
Sbjct:  287 QLEQVSGWSGMVFETVKALRDEQEPERMQGEWIDFRRDTELPKILFKKIRLSGNARVDQQKYPFVGLIKELSSSPSMYRQPIQLQAQVEAEAEIKFAGDLKFYEADPTHEFVVLFHFPHQKKIRIEDSDKLSMALIADQTECKSYVTLREHDFQCRLEFNQSPVRFEVSTSDTGNQMMAGIVQQTLASIDHITATLHCSGSYERPEVRLESELGEKVAQSLKLAFQSQLVKQKQDL 522          
BLAST of mRNA_M-pyrifera_M_contig97330.22419.1 vs. uniprot
Match: A0A517VH94_9PLAN (Uncharacterized protein n=1 Tax=Gimesia algae TaxID=2527971 RepID=A0A517VH94_9PLAN)

HSP 1 Score: 284 bits (727), Expect = 1.230e-89
Identity = 137/236 (58.05%), Postives = 188/236 (79.66%), Query Frame = 1
Query:    1 QLEEMTGWSSMIFQTVRTMRDEQEPERMQGEWIDFRPADALPNVLFKQIKVSGNARVNQQKYPFQALVRELSSEPTAYRRPIQLQARVEAEGEVRLAGELKFYEQQPTHDFLVKFDLPKQKTIQLENSDKLSLALIADQTECESRISFRENDFTCRVEFKQTPVRFQFDSEQAGIKPLTQVVAQALSTIDSISATLEGSGSYSQPVWRLESETGEQIARGLKLACQAEIGRQKQAL 708
            Q+E++ GW++ +FQTV+ M+DEQEPER+QGEWIDFR     P+VLFK I++SG AR +Q++ PF A+V+ LSS P  YR+PI++QA+V+ E E++ AGELKFYE +PTH+F+V F LP+QK I +ENSD++SLAL+ADQTEC S +SFRE+DF C++EF QTPVRF+FDS QA  + +T+++ Q+L++IDS+S  L GSGSY +P W++ES  G Q+ARGL  A QAEI R K+ L
Sbjct:  288 QIEQVFGWTNYMFQTVQAMQDEQEPERIQGEWIDFRSDVNEPDVLFKDIRLSGFARRDQEQIPFLAVVKNLSSTPRQYRQPIRIQAQVDGEAEIKFAGELKFYEAEPTHEFVVHFKLPQQKRITIENSDRISLALVADQTECRSHLSFREHDFQCQIEFSQTPVRFEFDSSQAETQSITRLLQQSLASIDSVSVKLHGSGSYDRPEWQIESALGAQVARGLTAAFQAEIARGKEKL 523          
BLAST of mRNA_M-pyrifera_M_contig97330.22419.1 vs. uniprot
Match: A0A2E2C295_9PLAN (Uncharacterized protein n=4 Tax=Planctomycetaceae TaxID=126 RepID=A0A2E2C295_9PLAN)

HSP 1 Score: 279 bits (713), Expect = 1.520e-87
Identity = 128/236 (54.24%), Postives = 188/236 (79.66%), Query Frame = 1
Query:    1 QLEEMTGWSSMIFQTVRTMRDEQEPERMQGEWIDFRPADALPNVLFKQIKVSGNARVNQQKYPFQALVRELSSEPTAYRRPIQLQARVEAEGEVRLAGELKFYEQQPTHDFLVKFDLPKQKTIQLENSDKLSLALIADQTECESRISFRENDFTCRVEFKQTPVRFQFDSEQAGIKPLTQVVAQALSTIDSISATLEGSGSYSQPVWRLESETGEQIARGLKLACQAEIGRQKQAL 708
            Q+E++ GW++ +FQTV+ M+DEQEPER+QGEWIDFR     P++LFK +++SG AR NQ + PF A+V+ELSS P  Y++PI++QA+++ + E+++AGELK+YE +P H+F+V F LP+QK I++ENSD++SLAL+ADQTEC S ++FRE+DF C++EF QTPVRF+FDS +   + +T+++ Q+LS ID+++  + GSGSY +P WR+ES  G+Q+ARGL  A QAE+ R KQ L
Sbjct:  288 QIEQVFGWTNFMFQTVQVMKDEQEPERIQGEWIDFRSDVNAPDILFKDLRISGFARRNQARIPFLAVVKELSSTPRQYQQPIRIQAQIDGQAEIKVAGELKYYEDEPAHEFVVLFKLPQQKKIKIENSDRISLALVADQTECRSHLTFREHDFQCQIEFSQTPVRFEFDSSRPEAQSITRLLQQSLSEIDTLAVNVHGSGSYDRPEWRIESALGQQVARGLTTAFQAELARGKQKL 523          
BLAST of mRNA_M-pyrifera_M_contig97330.22419.1 vs. uniprot
Match: A0A517WYA9_9PLAN (Uncharacterized protein n=2 Tax=Gimesia aquarii TaxID=2527964 RepID=A0A517WYA9_9PLAN)

HSP 1 Score: 273 bits (697), Expect = 3.720e-85
Identity = 136/234 (58.12%), Postives = 177/234 (75.64%), Query Frame = 1
Query:    1 QLEEMTGWSSMIFQTVRTMRDEQEPERMQGEWIDFRPADALPNVLFKQIKVSGNARVNQQKYPFQALVRELSSEPTAYRRPIQLQARVEAEGEVRLAGELKFYEQQPTHDFLVKFDLPKQKTIQLENSDKLSLALIADQTECESRISFRENDFTCRVEFKQTPVRFQFDSEQAGIKPLTQVVAQALSTIDSISATLEGSGSYSQPVWRLESETGEQIARGLKLACQAEIGRQKQ 702
            QL +++GWS+MI QTVRT++DEQEP+R QGEWIDFR   +LP VLFK+I+++G ARVNQQKYPF  +V+ LS  P  Y+ PI +QA+VEAE  +++AG+L FY++ PTH+F+V F LP QK I LENS+ LSL LIADQTEC++ ISFRE DF CR+EFKQTPVRFQ  S +     +  ++  +L +IDSISATL  SGSY  P  ++ESE G++IA GL +A + E  RQKQ
Sbjct:  288 QLNQVSGWSNMILQTVRTLQDEQEPDRSQGEWIDFRRDTSLPQVLFKKIRLTGAARVNQQKYPFVGMVKGLSCSPKLYQEPITIQAQVEAEAYIKMAGDLNFYQESPTHEFVVLFKLPHQKKITLENSEMLSLFLIADQTECKTHISFREEDFQCRLEFKQTPVRFQLSSPKNDHHAMGNILEHSLDSIDSISATLNYSGSYESPELKIESELGQKIAEGLNMALEVEFNRQKQ 521          
BLAST of mRNA_M-pyrifera_M_contig97330.22419.1 vs. uniprot
Match: A6CGY7_9PLAN (Uncharacterized protein n=6 Tax=Planctomycetaceae TaxID=126 RepID=A6CGY7_9PLAN)

HSP 1 Score: 268 bits (685), Expect = 2.300e-83
Identity = 128/236 (54.24%), Postives = 181/236 (76.69%), Query Frame = 1
Query:    1 QLEEMTGWSSMIFQTVRTMRDEQEPERMQGEWIDFRPADALPNVLFKQIKVSGNARVNQQKYPFQALVRELSSEPTAYRRPIQLQARVEAEGEVRLAGELKFYEQQPTHDFLVKFDLPKQKTIQLENSDKLSLALIADQTECESRISFRENDFTCRVEFKQTPVRFQFDSEQAGIKPLTQVVAQALSTIDSISATLEGSGSYSQPVWRLESETGEQIARGLKLACQAEIGRQKQAL 708
            Q+E++ GW++ +FQT + M+DEQEPER+QGEWIDFR     P+VLFK +++SG AR++Q+K PF A+V+ LSS P  Y +PIQ+QA+V+   EV+ AGELK+YE +P H+F+V F LP+QK I ++ SD++SLAL+ADQTEC + ++FRE DF CR+EF QTPVRF+ +S +   + + +++  +LS I+S+SAT+ GSGSY +P WR+ES  GEQ+ARGL  A QAEI R K+ L
Sbjct:  288 QVEQVLGWTNFMFQTAQAMKDEQEPERIQGEWIDFRSDVNEPDVLFKDLRLSGFARMDQEKIPFLAVVKGLSSTPRQYHQPIQIQAQVDGAAEVKFAGELKYYEVEPAHEFVVHFKLPQQKKITIQKSDRISLALVADQTECRTHLTFREQDFQCRIEFSQTPVRFELESTRPEAQSIARLLQHSLSAINSVSATVHGSGSYDRPEWRVESPLGEQVARGLTAAFQAEIARGKEKL 523          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig97330.22419.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 7
Match NameE-valueIdentityDescription
A0A2E7KEH3_9PLAN4.820e-15799.58Uncharacterized protein n=6 Tax=Gimesia TaxID=1649... [more]
A0A518A9U4_9PLAN5.570e-12578.81Uncharacterized protein n=3 Tax=Gimesia panareensi... [more]
A0A518IFZ0_9PLAN1.220e-9260.17Uncharacterized protein n=2 Tax=Gimesia TaxID=1649... [more]
A0A517VH94_9PLAN1.230e-8958.05Uncharacterized protein n=1 Tax=Gimesia algae TaxI... [more]
A0A2E2C295_9PLAN1.520e-8754.24Uncharacterized protein n=4 Tax=Planctomycetaceae ... [more]
A0A517WYA9_9PLAN3.720e-8558.12Uncharacterized protein n=2 Tax=Gimesia aquarii Ta... [more]
A6CGY7_9PLAN2.300e-8354.24Uncharacterized protein n=6 Tax=Planctomycetaceae ... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig97330contigM-pyrifera_M_contig97330:3..710 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score271.9
Seed ortholog evalue2.7e-70
Seed eggNOG ortholog344747.PM8797T_24156
KEGG koko:K09800
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Protein involved in outer membrane biogenesis
EggNOG OGs2J3X2@203682,COG2982@1,COG2982@2
COG Functional cat.M
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
BRITEko00000,ko02000
Exons1
Model size708
Cds size696
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816890.132855-CDS-M-pyrifera_M_contig97330:2..6981622816890.132855-CDS-M-pyrifera_M_contig97330:2..698Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig97330 3..698 -
1692278238.8446636-CDS-M-pyrifera_M_contig97330:2..6981692278238.8446636-CDS-M-pyrifera_M_contig97330:2..698Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig97330 3..698 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816890.144767-UTR-M-pyrifera_M_contig97330:698..7101622816890.144767-UTR-M-pyrifera_M_contig97330:698..710Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig97330 699..710 -
1692278238.8578503-UTR-M-pyrifera_M_contig97330:698..7101692278238.8578503-UTR-M-pyrifera_M_contig97330:698..710Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig97330 699..710 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig97330.22419.1prot_M-pyrifera_M_contig97330.22419.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig97330 3..698 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig97330.22419.1

>prot_M-pyrifera_M_contig97330.22419.1 ID=prot_M-pyrifera_M_contig97330.22419.1|Name=mRNA_M-pyrifera_M_contig97330.22419.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=232bp
MTGWSSMIFQTVRTMRDEQEPERMQGEWIDFRPADALPNVLFKQIKVSGN
ARVNQQKYPFQALVRELSSEPTAYRRPIQLQARVEAEGEVRLAGELKFYE
QQPTHDFLVKFDLPKQKTIQLENSDKLSLALIADQTECESRISFRENDFT
CRVEFKQTPVRFQFDSEQAGIKPLTQVVAQALSTIDSISATLEGSGSYSQ
PVWRLESETGEQIARGLKLACQAEIGRQKQAL
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mRNA from alignment at M-pyrifera_M_contig97330:3..710-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig97330.22419.1 ID=mRNA_M-pyrifera_M_contig97330.22419.1|Name=mRNA_M-pyrifera_M_contig97330.22419.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=708bp|location=Sequence derived from alignment at M-pyrifera_M_contig97330:3..710- (Macrocystis pyrifera P11B4 male)
CAGCTGGAAGAGATGACCGGCTGGTCGAGTATGATCTTTCAGACTGTCAG AACGATGCGCGACGAACAGGAGCCGGAGCGGATGCAGGGGGAATGGATCG ACTTCCGACCCGCGGATGCACTGCCGAATGTGTTATTTAAGCAGATCAAA GTGAGTGGAAACGCACGTGTGAATCAACAGAAATATCCGTTTCAGGCACT CGTGCGGGAACTCTCCTCTGAGCCGACGGCGTATCGCCGTCCGATTCAGC TACAGGCACGCGTCGAGGCGGAAGGAGAAGTCAGGCTTGCGGGAGAACTC AAATTTTATGAGCAGCAGCCGACACACGACTTCCTCGTCAAGTTTGACCT GCCGAAACAGAAAACGATTCAACTGGAGAATTCCGATAAGTTATCACTGG CACTGATCGCGGATCAGACCGAGTGCGAGTCGCGGATTTCGTTTCGGGAA AATGATTTCACGTGCCGGGTGGAATTTAAACAGACTCCCGTGCGGTTTCA GTTCGATTCCGAGCAGGCAGGCATCAAGCCTCTGACCCAAGTCGTCGCCC AGGCGCTCTCCACCATCGATTCCATCTCAGCGACCCTGGAGGGATCGGGA AGTTACAGCCAGCCTGTCTGGCGGCTGGAATCGGAAACCGGCGAGCAGAT TGCCCGTGGGTTGAAGCTGGCCTGCCAGGCGGAAATCGGTCGTCAGAAGC AGGCACTG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig97330:3..710-

>mRNA_M-pyrifera_M_contig97330.22419.1 ID=mRNA_M-pyrifera_M_contig97330.22419.1|Name=mRNA_M-pyrifera_M_contig97330.22419.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1392bp|location=Sequence derived from alignment at M-pyrifera_M_contig97330:3..710- (Macrocystis pyrifera P11B4 male)
ATGACCGGCTGGTCGAGTATGATCTTTCAGACTGTCAGAACGATGCGCGA
CGAACAGGAGCCGGAGCGGATGCAGGGGGAATGGATCGACTTCCGACCCG
CGGATGCACTGCCGAATGTGTTATTTAAGCAGATCAAAGTGAGTGGAAAC
GCACGTGTGAATCAACAGAAATATCCGTTTCAGGCACTCGTGCGGGAACT
CTCCTCTGAGCCGACGGCGTATCGCCGTCCGATTCAGCTACAGGCACGCG
TCGAGGCGGAAGGAGAAGTCAGGCTTGCGGGAGAACTCAAATTTTATGAG
CAGCAGCCGACACACGACTTCCTCGTCAAGTTTGACCTGCCGAAACAGAA
AACGATTCAACTGGAGAATTCCGATAAGTTATCACTGGCACTGATCGCGG
ATCAGACCGAGTGCGAGTCGCGGATTTCGTTTCGGGAAAATGATTTCACG
TGCCGGGTGGAATTTAAACAGACTCCCGTGCGGTTTCAGTTCGATTCCGA
GCAGGCAGGCATCAAGCCTCTGACCCAAGTCGTCGCCCAGGCGCTCTCCA
CCATCGATTCCATCTCAGCGACCCTGGAGGGATCGGGAAGTTACAGCCAG
CCTGTCTGGCGGCTGGAATCGGAAACCGGCGAGCAGATTGCCCGTGGGTT
GAAGCTGGCCTGCCAGGCGGAAATCGGTCGTCAGAAGCAGGCACTGATGA
CCGGCTGGTCGAGTATGATCTTTCAGACTGTCAGAACGATGCGCGACGAA
CAGGAGCCGGAGCGGATGCAGGGGGAATGGATCGACTTCCGACCCGCGGA
TGCACTGCCGAATGTGTTATTTAAGCAGATCAAAGTGAGTGGAAACGCAC
GTGTGAATCAACAGAAATATCCGTTTCAGGCACTCGTGCGGGAACTCTCC
TCTGAGCCGACGGCGTATCGCCGTCCGATTCAGCTACAGGCACGCGTCGA
GGCGGAAGGAGAAGTCAGGCTTGCGGGAGAACTCAAATTTTATGAGCAGC
AGCCGACACACGACTTCCTCGTCAAGTTTGACCTGCCGAAACAGAAAACG
ATTCAACTGGAGAATTCCGATAAGTTATCACTGGCACTGATCGCGGATCA
GACCGAGTGCGAGTCGCGGATTTCGTTTCGGGAAAATGATTTCACGTGCC
GGGTGGAATTTAAACAGACTCCCGTGCGGTTTCAGTTCGATTCCGAGCAG
GCAGGCATCAAGCCTCTGACCCAAGTCGTCGCCCAGGCGCTCTCCACCAT
CGATTCCATCTCAGCGACCCTGGAGGGATCGGGAAGTTACAGCCAGCCTG
TCTGGCGGCTGGAATCGGAAACCGGCGAGCAGATTGCCCGTGGGTTGAAG
CTGGCCTGCCAGGCGGAAATCGGTCGTCAGAAGCAGGCACTG
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