mRNA_M-pyrifera_M_contig96201.22171.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig96201.22171.1
Unique NamemRNA_M-pyrifera_M_contig96201.22171.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig96201.22171.1 vs. uniprot
Match: A0A6I3F7P9_9ACTN (LLM class flavin-dependent oxidoreductase n=2 Tax=root TaxID=1 RepID=A0A6I3F7P9_9ACTN)

HSP 1 Score: 152 bits (385), Expect = 1.980e-42
Identity = 77/142 (54.23%), Postives = 93/142 (65.49%), Query Frame = 1
Query:   46 YVHMVNGTVLDEGESPTADRVVDAAGPWEALSHHGAWHLAGEEIVPMLPGGAEWLERVVADRPEEERHLSVHEWHCTHVNDRDRPLLATKGTADDPWSGWVGDDADIASRIAASGDAGSSEILYTPSGPDLEREMRAFARAS 471
            +V MV+GTVLD GE   A RV +A GPW  + +H  W    E +  M PGG EWL RV +DRPE ERHL+V+E H T ++DRDRPLL          +GWVGD  DIA R+  S   G  E+LYTPSGPD+EREMRAFA A+
Sbjct:  188 HVQMVSGTVLDPGEDVGASRVREAVGPWFVVGYHYVWQAFPEALAGM-PGGPEWLARVESDRPEGERHLAVNEGHVTDISDRDRPLLEAASAETIGAAGWVGDATDIARRVGESAGRGVDEVLYTPSGPDVEREMRAFAAAA 328          
BLAST of mRNA_M-pyrifera_M_contig96201.22171.1 vs. uniprot
Match: A0A7C4TJA4_9ACTN (LLM class flavin-dependent oxidoreductase n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A7C4TJA4_9ACTN)

HSP 1 Score: 151 bits (381), Expect = 7.280e-42
Identity = 75/140 (53.57%), Postives = 95/140 (67.86%), Query Frame = 1
Query:   49 VHMVNGTVLDEGESPTADRVVDAAGPWEALSHHGAWHLAGEEIVPMLPGGAEWLERVVADRPEEERHLSVHEWHCTHVNDRDRPLLATKGTADDPWSGWVGDDADIASRIAASGDAGSSEILYTPSGPDLEREMRAFARA 468
            + MVNGTVLD+ ES    RV +A GPW  L++HG W  AG+  V  LPGGAEW   + A+RPE ERHL+VHE H THV  RDRPLL   G     W GWVG   ++ +R  A+  +G +++LYTP+GPD+ER+MRAFA A
Sbjct:  187 IQMVNGTVLDDDESIETPRVREALGPWYVLAYHGTWQTAGD-AVDGLPGGAEWRAAIEAERPEGERHLAVHEGHATHVMPRDRPLLDAAGDGLAGW-GWVGTPEEVRARAEAAAASGVTDLLYTPAGPDVERDMRAFAAA 324          
BLAST of mRNA_M-pyrifera_M_contig96201.22171.1 vs. uniprot
Match: UPI001E391C73 (LLM class flavin-dependent oxidoreductase n=1 Tax=Rhabdothermincola sp. EGI L10124 TaxID=2903142 RepID=UPI001E391C73)

HSP 1 Score: 148 bits (374), Expect = 8.620e-41
Identity = 78/141 (55.32%), Postives = 94/141 (66.67%), Query Frame = 1
Query:   46 YVHMVNGTVLDEGESPTADRVVDAAGPWEALSHHGAWHLAGEEIVPMLPGGAEWLERVVADRPEEERHLSVHEWHCTHVNDRDRPLLATKGTADDPWSGWVGDDADIASRIAASGDAGSSEILYTPSGPDLEREMRAFARA 468
            +V MV+GTVLD GESP   R  +A GPW  +S+H  W    E +  M P GAEWL RV ADRPE ERHL+VHE H T V DRDR +L          +GWVG+  DIA+R+ AS   G +E+LYTP+GPD+EREMRAFA A
Sbjct:  188 HVQMVSGTVLDPGESPGDPRAREAIGPWYVVSYHYVWTQFREALEGM-PAGAEWLARVEADRPEGERHLAVHEGHVTEVTDRDRMVLEAATAEALGGAGWVGEADDIAARVKASEANGVTEVLYTPAGPDVEREMRAFAAA 327          
BLAST of mRNA_M-pyrifera_M_contig96201.22171.1 vs. uniprot
Match: A0A2E8K911_9GAMM (Bac_luciferase domain-containing protein n=2 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A2E8K911_9GAMM)

HSP 1 Score: 143 bits (361), Expect = 7.260e-39
Identity = 65/150 (43.33%), Postives = 95/150 (63.33%), Query Frame = 1
Query:   19 GPEGAVPEEYVHMVNGTVLDEGESPTADRVVDAAGPWEALSHHGAWHLAGEEIVPMLPGGAEWLERVVADRPEEERHLSVHEWHCTHVNDRDRPLLATKGTADDPWSGWVGDDADIASRIAASGDAGSSEILYTPSGPDLEREMRAFARA 468
            GP     + Y+ MV+GTVL+ GE P + R +DAAGPW    +HG W  AG E +  +PGG  WL ++  DRPE +RHL+VHE HCTH+ +RD+ L+   G+  + W+GWVG+   I   ++A  + G++EI++   G +  RE+ AFA A
Sbjct:  178 GPSDGSWDSYIQMVHGTVLEAGEDPLSQRALDAAGPWWTPVYHGMWSAAGPESLGEVPGGEAWLAQIAKDRPEGQRHLAVHEGHCTHITERDQILIDASGSKLE-WTGWVGEPEQIREFVSAEAETGTTEIIFNSGGQNPLREIEAFAEA 326          
BLAST of mRNA_M-pyrifera_M_contig96201.22171.1 vs. uniprot
Match: A0A381ZYE8_9ZZZZ (Bac_luciferase domain-containing protein (Fragment) n=2 Tax=root TaxID=1 RepID=A0A381ZYE8_9ZZZZ)

HSP 1 Score: 141 bits (356), Expect = 3.440e-38
Identity = 69/151 (45.70%), Postives = 97/151 (64.24%), Query Frame = 1
Query:   19 GPEGAVPEEYVHMVNGTVLDEGESPTADRVVDAAGPWEALSHHGAWHLAGEEIVPMLPGGAEWLERVVADRPEEERHLSVHEWHCTHVNDRDRPLLATKGTADDPWSGWVGDDADIASRIAASGDAGSSEILYTPSGPDLEREMRAFARAS 471
            GP       +V MV+GTVL+ GE P ++RV+DAAGPW  +SHH +W  A  + +  +PGG  +LE++  DRP E  HL+VHE H TH+ DRDR L+       D    WVGD   +  RI+ + ++G++EI+Y P+GPD  RE+RAFA A+
Sbjct:  171 GPGDGSWNTFVQMVHGTVLESGEDPNSNRVIDAAGPWWVMSHHSSWE-ASPQRLQTVPGGPAYLEQLEIDRPPEAAHLAVHEGHATHLTDRDRILVDAASDGFDWSRSWVGDIGYMRERISEAAESGTTEIIYNPAGPDPLREIRAFAEAA 320          
BLAST of mRNA_M-pyrifera_M_contig96201.22171.1 vs. uniprot
Match: A0A2E3NEW5_9DELT (LLM class flavin-dependent oxidoreductase n=1 Tax=Deltaproteobacteria bacterium TaxID=2026735 RepID=A0A2E3NEW5_9DELT)

HSP 1 Score: 142 bits (358), Expect = 4.730e-38
Identity = 71/140 (50.71%), Postives = 91/140 (65.00%), Query Frame = 1
Query:   49 VHMVNGTVLDEGESPTADRVVDAAGPWEALSHHGAWHLAGEEIVPMLPGGAEWLERVVADRPEEERHLSVHEWHCTHVNDRDRPLLATKGTADDPWSGWVGDDADIASRIAASGDAGSSEILYTPSGPDLEREMRAFARA 468
            VHMVNGTVL  GESP+A RV+D AGPW   ++H  W       +P +PGGA+WL ++ ++R E ERHL+VH  HC+H+ND DR  +A  G A  PW GWVG+   +  R   S  AG+SEILYTP+G DL  +   F RA
Sbjct:  226 VHMVNGTVLAPGESPSAPRVLDEAGPWLVGAYHMGWE-TNPSGLPDMPGGADWLAQIESERDEAERHLAVHSGHCSHLNDADRKAVAAYGDAV-PWWGWVGEAEAVRKRAEESAAAGTSEILYTPAGDDLLGQAETFFRA 363          
BLAST of mRNA_M-pyrifera_M_contig96201.22171.1 vs. uniprot
Match: A0A2E1WN34_9ACTN (Bac_luciferase domain-containing protein n=3 Tax=Acidimicrobiia TaxID=84992 RepID=A0A2E1WN34_9ACTN)

HSP 1 Score: 139 bits (351), Expect = 2.220e-37
Identity = 68/152 (44.74%), Postives = 100/152 (65.79%), Query Frame = 1
Query:   19 GPEGAVPEEYVHMVNGTVLDEGESPTADRVVDAAGPWEALSHHGAWHLAGEEIVPMLPGGAEWLERVVADRPEEERHLSVHEWHCTHVNDRDRPLLATKGTADDPWS-GWVGDDADIASRIAASGDAGSSEILYTPSGPDLEREMRAFARAS 471
            GP       +V MV+GTV++ GE+PT+DRV++AAGPW  +SHH +W ++ E +   +PGG  +LE++  +RP +  HL+VHE H TH+ DRDR +L      D  W   WVGD   I  RI+   ++G++EI+Y P+GPD  RE+R+FA A+
Sbjct:  178 GPGDGSWNTFVQMVHGTVVEPGENPTSDRVIEAAGPWWVMSHHSSWEVSPERL-QTVPGGPTYLEQLDIERPADALHLAVHEGHATHLTDRDR-ILVDAAREDFEWHRSWVGDSGYIRERISEVAESGTTEIIYNPAGPDPLREIRSFAEAA 327          
BLAST of mRNA_M-pyrifera_M_contig96201.22171.1 vs. uniprot
Match: A0A7K1A4M4_9ACTN (LLM class flavin-dependent oxidoreductase n=9 Tax=root TaxID=1 RepID=A0A7K1A4M4_9ACTN)

HSP 1 Score: 139 bits (349), Expect = 8.100e-37
Identity = 77/158 (48.73%), Postives = 96/158 (60.76%), Query Frame = 1
Query:    1 MNVAP-PGPEGAVPEEYVHMVNGTVLDEGESPTADRVVDAAGPWEALSHHGAWHLAGEEIVPMLPGGAEWLERVVADRPEEERHLSVHEWHCTHVNDRDRPLLATKGTADDPWSGWVGDDADIASRIAASGDAGSSEILYTPSGPDLEREMRAFARAS 471
            + VAP  GP G      V MV+G+VLDEGES  + R   A GPW  + +HG W  A  E +  +PGGAEWL  V + RPE ERHL+VHE H T V  RD+ +L     A     GW GD A I  +++ +   G  EILYTP+GPD+EREMRAFA A+
Sbjct:  204 IGVAPLEGPWG----HQVLMVSGSVLDEGESAGSPRARAAIGPWYVVGYHGCWE-AAPEFLTAMPGGAEWLADVESTRPERERHLAVHEGHVTEVFGRDQVVLDLADEATLSGVGWTGDSASIKEKVSHASSVGVREILYTPAGPDVEREMRAFAAAT 356          
BLAST of mRNA_M-pyrifera_M_contig96201.22171.1 vs. uniprot
Match: A0A2E7XJG6_9GAMM (LLM class flavin-dependent oxidoreductase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A2E7XJG6_9GAMM)

HSP 1 Score: 138 bits (347), Expect = 1.570e-36
Identity = 65/144 (45.14%), Postives = 93/144 (64.58%), Query Frame = 1
Query:   40 EEYVHMVNGTVLDEGESPTADRVVDAAGPWEALSHHGAWHLAGEEIVPMLPGGAEWLERVVADRPEEERHLSVHEWHCTHVNDRDRPLLATKGTADDPWSGWVGDDADIASRIAASGDAGSSEILYTPSGPDLEREMRAFARAS 471
            ++++ MV+GTVL+ GESP ++R +DAAGPW  +  H    + G E +   PGG E+L++V ADR E +RHL++HE HCTH+ +RDR L+   G     W GWVG+ A+I         AG SE+ + P+G D  RE+RAFA A+
Sbjct:  207 DQFIRMVDGTVLEPGESPHSERAIDAAGPWWVMGAHATLEMEGAEALGKWPGGDEYLDQVAADRLEGQRHLAIHEGHCTHLTERDRILIDAVG-GKPQWRGWVGEAAEIKRWAEEEAAAGVSEVFFVPAGSDPLREVRAFAEAT 349          
BLAST of mRNA_M-pyrifera_M_contig96201.22171.1 vs. uniprot
Match: A0A6I2X9K6_9ACTN (LLM class flavin-dependent oxidoreductase n=2 Tax=root TaxID=1 RepID=A0A6I2X9K6_9ACTN)

HSP 1 Score: 135 bits (340), Expect = 9.740e-36
Identity = 69/141 (48.94%), Postives = 89/141 (63.12%), Query Frame = 1
Query:   49 VHMVNGTVLDEGESPTADRVVDAAGPWEALSHHGAWHLAGEEIVPMLPGGAEWLERVVADRPEEERHLSVHEWHCTHVNDRDRPLLATKGTADDPWSGWVGDDADIASRIAASGDAGSSEILYTPSGPDLEREMRAFARAS 471
            V MV+GTVLD GE  ++ RV DA GPW  + HH  W     E++  +P G+EWL  + ADRPE ERHL VHE H T V+ RDR ++          +GW+G+   IA R  AS   G +E+LYTP+G D+EREMRAFA A+
Sbjct:  189 VQMVSGTVLDRGEDGSSPRVRDAVGPWFVVGHHYVWQQF-PEVLSAMPAGSEWLAGIEADRPEGERHLIVHEGHVTDVSARDRQIIDAATDESILGAGWIGEKDAIAERAGASEARGVTELLYTPAGSDVEREMRAFAAAT 328          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig96201.22171.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6I3F7P9_9ACTN1.980e-4254.23LLM class flavin-dependent oxidoreductase n=2 Tax=... [more]
A0A7C4TJA4_9ACTN7.280e-4253.57LLM class flavin-dependent oxidoreductase n=1 Tax=... [more]
UPI001E391C738.620e-4155.32LLM class flavin-dependent oxidoreductase n=1 Tax=... [more]
A0A2E8K911_9GAMM7.260e-3943.33Bac_luciferase domain-containing protein n=2 Tax=G... [more]
A0A381ZYE8_9ZZZZ3.440e-3845.70Bac_luciferase domain-containing protein (Fragment... [more]
A0A2E3NEW5_9DELT4.730e-3850.71LLM class flavin-dependent oxidoreductase n=1 Tax=... [more]
A0A2E1WN34_9ACTN2.220e-3744.74Bac_luciferase domain-containing protein n=3 Tax=A... [more]
A0A7K1A4M4_9ACTN8.100e-3748.73LLM class flavin-dependent oxidoreductase n=9 Tax=... [more]
A0A2E7XJG6_9GAMM1.570e-3645.14LLM class flavin-dependent oxidoreductase n=1 Tax=... [more]
A0A6I2X9K6_9ACTN9.740e-3648.94LLM class flavin-dependent oxidoreductase n=2 Tax=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig96201contigM-pyrifera_M_contig96201:5..487 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score130.2
Seed ortholog evalue8.8e-28
Seed eggNOG ortholog298655.KI912267_gene7294
Hectar predicted targeting categoryother localisation
EggNOG free text desc.COG2141 Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases
EggNOG OGsCOG2141@1,COG2141@2
COG Functional cat.C
Best tax levelBacteria
Best eggNOG OGNA|NA|NA
Exons1
Model size483
Cds size483
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816877.1776874-CDS-M-pyrifera_M_contig96201:4..4871622816877.1776874-CDS-M-pyrifera_M_contig96201:4..487Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig96201 5..487 +
1692278231.34015-CDS-M-pyrifera_M_contig96201:4..4871692278231.34015-CDS-M-pyrifera_M_contig96201:4..487Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig96201 5..487 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig96201.22171.1prot_M-pyrifera_M_contig96201.22171.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig96201 5..487 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig96201.22171.1

>prot_M-pyrifera_M_contig96201.22171.1 ID=prot_M-pyrifera_M_contig96201.22171.1|Name=mRNA_M-pyrifera_M_contig96201.22171.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=161bp
MNVAPPGPEGAVPEEYVHMVNGTVLDEGESPTADRVVDAAGPWEALSHHG
AWHLAGEEIVPMLPGGAEWLERVVADRPEEERHLSVHEWHCTHVNDRDRP
LLATKGTADDPWSGWVGDDADIASRIAASGDAGSSEILYTPSGPDLEREM
RAFARASGLG*
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mRNA from alignment at M-pyrifera_M_contig96201:5..487+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig96201.22171.1 ID=mRNA_M-pyrifera_M_contig96201.22171.1|Name=mRNA_M-pyrifera_M_contig96201.22171.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=483bp|location=Sequence derived from alignment at M-pyrifera_M_contig96201:5..487+ (Macrocystis pyrifera P11B4 male)
ATGAACGTCGCACCCCCGGGCCCCGAGGGAGCCGTGCCCGAGGAGTACGT CCACATGGTCAACGGCACGGTGCTCGACGAGGGCGAATCGCCCACCGCCG ACCGAGTCGTCGACGCGGCGGGTCCGTGGGAAGCGCTCTCGCACCATGGC GCCTGGCATCTCGCCGGTGAGGAGATCGTGCCGATGCTGCCCGGCGGGGC CGAGTGGCTCGAGCGAGTCGTGGCCGACCGCCCGGAGGAAGAACGTCACC TGTCGGTGCACGAGTGGCACTGCACCCATGTCAACGATCGTGACCGGCCG TTGCTGGCGACCAAGGGCACCGCAGACGACCCGTGGTCCGGCTGGGTGGG CGACGACGCAGACATCGCGTCGCGCATCGCGGCCAGCGGCGACGCCGGCT CGAGCGAGATCCTCTACACGCCGTCGGGACCCGATCTCGAACGTGAGATG CGAGCCTTCGCCCGGGCGTCCGGCCTCGGCTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig96201:5..487+

>mRNA_M-pyrifera_M_contig96201.22171.1 ID=mRNA_M-pyrifera_M_contig96201.22171.1|Name=mRNA_M-pyrifera_M_contig96201.22171.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=966bp|location=Sequence derived from alignment at M-pyrifera_M_contig96201:5..487+ (Macrocystis pyrifera P11B4 male)
ATGAACGTCGCACCCCCGGGCCCCGAGGGAGCCGTGCCCGAGGAGTACGT
CCACATGGTCAACGGCACGGTGCTCGACGAGGGCGAATCGCCCACCGCCG
ACCGAGTCGTCGACGCGGCGGGTCCGTGGGAAGCGCTCTCGCACCATGGC
GCCTGGCATCTCGCCGGTGAGGAGATCGTGCCGATGCTGCCCGGCGGGGC
CGAGTGGCTCGAGCGAGTCGTGGCCGACCGCCCGGAGGAAGAACGTCACC
TGTCGGTGCACGAGTGGCACTGCACCCATGTCAACGATCGTGACCGGCCG
TTGCTGGCGACCAAGGGCACCGCAGACGACCCGTGGTCCGGCTGGGTGGG
CGACGACGCAGACATCGCGTCGCGCATCGCGGCCAGCGGCGACGCCGGCT
CGAGCGAGATCCTCTACACGCCGTCGGGACCCGATCTCGAACGTGAGATG
CGAGCCTTCGCCCGGGCGTCCGGCCTCGGCTGAATGAACGTCGCACCCCC
GGGCCCCGAGGGAGCCGTGCCCGAGGAGTACGTCCACATGGTCAACGGCA
CGGTGCTCGACGAGGGCGAATCGCCCACCGCCGACCGAGTCGTCGACGCG
GCGGGTCCGTGGGAAGCGCTCTCGCACCATGGCGCCTGGCATCTCGCCGG
TGAGGAGATCGTGCCGATGCTGCCCGGCGGGGCCGAGTGGCTCGAGCGAG
TCGTGGCCGACCGCCCGGAGGAAGAACGTCACCTGTCGGTGCACGAGTGG
CACTGCACCCATGTCAACGATCGTGACCGGCCGTTGCTGGCGACCAAGGG
CACCGCAGACGACCCGTGGTCCGGCTGGGTGGGCGACGACGCAGACATCG
CGTCGCGCATCGCGGCCAGCGGCGACGCCGGCTCGAGCGAGATCCTCTAC
ACGCCGTCGGGACCCGATCTCGAACGTGAGATGCGAGCCTTCGCCCGGGC
GTCCGGCCTCGGCTGA
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