mRNA_M-pyrifera_M_contig96111.22154.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig96111.22154.1
Unique NamemRNA_M-pyrifera_M_contig96111.22154.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig96111.22154.1 vs. uniprot
Match: A0A2E7MAX7_9DELT (Thioesterase n=1 Tax=Deltaproteobacteria bacterium TaxID=2026735 RepID=A0A2E7MAX7_9DELT)

HSP 1 Score: 134 bits (338), Expect = 1.400e-37
Identity = 70/142 (49.30%), Postives = 96/142 (67.61%), Query Frame = 1
Query:   52 MGRLVPFNGFVGLEFREVTAEHVQLELPHRPEHIGDPTRPALHGGALAMLADTAGGTLVLAATEAGDKVSTLDMRIDYLRPADLTTTFADATLVRLGRRAAVVRIHVWQP-------SDTEPDG---KRHIADSTAVYSVYR 447
            M + +PFN F+G+E   + +    L+LP R + IGDP RPALHGG LAMLADTAGG  V +A + GD +ST+D+R+DYLRPADL  T A+A +VR G R AV R+ +WQ        S+T+  G   +R +A++TAVY++ R
Sbjct:    8 MEQQIPFNAFLGIEVVALESGSAALKLPFREQLIGDPVRPALHGGTLAMLADTAGGAAVFSAIQFGDTISTIDLRVDYLRPADLLDTVAEARVVRQGGRVAVARVELWQDCVDTHGASETQSAGVGDRRLVAEATAVYNIRR 149          
BLAST of mRNA_M-pyrifera_M_contig96111.22154.1 vs. uniprot
Match: A0A2E6EKQ5_9RICK (Thioesterase n=1 Tax=Rickettsiales bacterium TaxID=2026788 RepID=A0A2E6EKQ5_9RICK)

HSP 1 Score: 131 bits (329), Expect = 5.790e-36
Identity = 73/140 (52.14%), Postives = 89/140 (63.57%), Query Frame = 1
Query:   64 VPFNGFVGLEFREVTAEHVQLELPHRPEHIGDPTRPALHGGALAMLADTAGGTLVLAATEAGDKVSTLDMRIDYLRPADLTTTFADATLVRLGRRAAVVRIHVWQPSDT----------EPDGKRHIADSTAVYSVYRAE 453
            +PFN  +GLE RE+      L LP +P  IGDP RPALHGG+++MLADTAGG  VLA  +AG  VST+D+RIDYLRP  L    A+A LVRLG R  VVRI V+Q S+           E    R IA++T VYSV R +
Sbjct:   33 IPFNRHLGLEVRELAVGRAVLALPFQPAFIGDPFRPALHGGSISMLADTAGGAAVLAGVDAGSPVSTIDLRIDYLRPGALVDLVAEAALVRLGGRVGVVRIQVYQESNEGNASEEARIDEEGTCRVIAEATGVYSVRRPQ 172          
BLAST of mRNA_M-pyrifera_M_contig96111.22154.1 vs. uniprot
Match: A0A356KLU1_9BACT (Thioesterase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A356KLU1_9BACT)

HSP 1 Score: 124 bits (310), Expect = 1.810e-33
Identity = 59/130 (45.38%), Postives = 81/130 (62.31%), Query Frame = 1
Query:   64 VPFNGFVGLEFREVTAEHVQLELPHRPEHIGDPTRPALHGGALAMLADTAGGTLVLAATEAGDKVSTLDMRIDYLRPADLTTTFADATLVRLGRRAAVVRIHVWQPSDTEPDGKRHIADSTAVYSVYRAE 453
            +PFN  VGLE  E+        + +RPE IGDPTRPA+HGG ++MLADT GG+ V + T  GD+V+T+D+R+DYLRP       A+AT++R G R  V  + VWQ    E      IA + AVY++ R +
Sbjct:   12 IPFNAHVGLELLELERGRALARIAYRPELIGDPTRPAIHGGVISMLADTVGGSAVFSLTAPGDRVATIDLRVDYLRPGKPADLLAEATVIRAGNRVGVASVEVWQGESREAPRDALIAVAKAVYTIKRGD 141          
BLAST of mRNA_M-pyrifera_M_contig96111.22154.1 vs. uniprot
Match: A0A0F6YKF8_9DELT (Phenylacetic acid degradation-related protein n=2 Tax=Sandaracinus amylolyticus TaxID=927083 RepID=A0A0F6YKF8_9DELT)

HSP 1 Score: 119 bits (297), Expect = 2.050e-31
Identity = 68/150 (45.33%), Postives = 91/150 (60.67%), Query Frame = 1
Query:    1 DEVERARTDRVFAAELIMGRLVPFNGFVGLEFREVTAEHVQLELPHRPEHIGDPTRPALHGGALAMLADTAGGTLVLAATEAGDKVSTLDMRIDYLRPADLTTTFADATLVRLGRRAAVVRIHVWQPSDTEPDGKRHIADSTAVYSVYRA 450
            DE+ER    R+     +M   +PFN  +GL  RE+T     LE+P++PE IGDP RPALHGG L+ +ADTAGG  V       D+VST+D+R+DYLRP  L    A A+++R+G R  V  + +  PS   PD    IA++  VYSV RA
Sbjct:    6 DELER----RLAFVRRLMDEAIPFNRVLGLRVRELTRGRAVLEVPYKPELIGDPDRPALHGGVLSAVADTAGGCAVWTTVGESDRVSTIDLRVDYLRPGRLEPFHAIASVLRVGNRVGVANVLLVHPS--APD--EPIAEAKGVYSVKRA 147          
BLAST of mRNA_M-pyrifera_M_contig96111.22154.1 vs. uniprot
Match: A0A3M1TR86_9BACT (Hotdog fold thioesterase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3M1TR86_9BACT)

HSP 1 Score: 118 bits (295), Expect = 4.240e-31
Identity = 61/128 (47.66%), Postives = 79/128 (61.72%), Query Frame = 1
Query:   64 VPFNGFVGLEFREVTAEHVQLELPHRPEHIGDPTRPALHGGALAMLADTAGGTLVLAATEAGDKVSTLDMRIDYLRPADLTTTFADATLVRLGRRAAVVRIHVWQPSDTEPDGKRHIADSTAVYSVYR 447
            +PFN F+GL  REV    V  ELP RPE IG+P RPALHGG ++MLADT GG  +   T+ GD+++T+D+R+DYLRP       A+  +VR G R  V  I V  P D EP     IA +  VY++ R
Sbjct:   25 IPFNAFLGLRLREVDRGRVLAELPFRPEFIGNPFRPALHGGVISMLADTVGGASLYTLTDPGDQLATIDLRVDYLRPGRPEDLLAEGQVVRWGNRVGVSSIAVHHPGDDEP-----IAVAKGVYNIKR 147          
BLAST of mRNA_M-pyrifera_M_contig96111.22154.1 vs. uniprot
Match: A0A2M8B8M8_9DELT (Thioesterase n=2 Tax=unclassified Deltaproteobacteria TaxID=122706 RepID=A0A2M8B8M8_9DELT)

HSP 1 Score: 117 bits (292), Expect = 1.070e-30
Identity = 57/132 (43.18%), Postives = 82/132 (62.12%), Query Frame = 1
Query:   52 MGRLVPFNGFVGLEFREVTAEHVQLELPHRPEHIGDPTRPALHGGALAMLADTAGGTLVLAATEAGDKVSTLDMRIDYLRPADLTTTFADATLVRLGRRAAVVRIHVWQPSDTEPDGKRHIADSTAVYSVYR 447
            M   +PFN  +GL+  E+ +  + +E+P  P  +GD  RPALHGG ++MLADTAGG  V    + G ++ST+D+R+DYLRPA L    A A ++RLG R  V ++ V+Q       G  H+AD  AVY++ R
Sbjct:   14 MEEQIPFNVHLGLKISELESGRITMEIPFAPHLVGDSDRPALHGGVISMLADTAGGAAVFTKVDPGSRLSTVDLRVDYLRPARLEALCATAEVIRLGNRVGVAQVRVFQ-------GSLHVADGRAVYNIRR 138          
BLAST of mRNA_M-pyrifera_M_contig96111.22154.1 vs. uniprot
Match: A0A521TTS0_9DELT (Hotdog fold thioesterase n=1 Tax=Myxococcaceae bacterium TaxID=2484252 RepID=A0A521TTS0_9DELT)

HSP 1 Score: 114 bits (284), Expect = 2.000e-29
Identity = 60/151 (39.74%), Postives = 87/151 (57.62%), Query Frame = 1
Query:    1 DEVERARTDRVFAAELIMGRLVPFNGFVGLEFREVTAEHVQLELPHRPEHIGDPTRPALHGGALAMLADTAGGTLVLAATEAGDKVSTLDMRIDYLRPADLTTTFADATLVRLGRRAAVVRIHVWQPSDTEPDGKRHIADSTAVYSVYRAE 453
            D   R   +R FA E      +PFN ++GL    +     ++ LP +PE +GDP RPALHGG +A   DT GG  V +    GD+VST+D+R+DYLRP  L   +A+AT+ R+G R  V  I  +  SD E    + +A++ AVY++ R +
Sbjct:    9 DPAMRGEIERFFAEE------IPFNRYLGLRLVSLERGVARVLLPFKPELVGDPMRPALHGGVIAAALDTVGGAAVFSELAVGDRVSTVDIRVDYLRPGRLEDLYAEATIKRVGNRMGVTAIRAFHESDPE----QSVAEAMAVYNIRRVQ 149          
BLAST of mRNA_M-pyrifera_M_contig96111.22154.1 vs. uniprot
Match: A0A847UXX3_9DELT (PaaI family thioesterase n=1 Tax=Desulfovibrionales bacterium TaxID=2448050 RepID=A0A847UXX3_9DELT)

HSP 1 Score: 113 bits (283), Expect = 2.750e-29
Identity = 60/134 (44.78%), Postives = 85/134 (63.43%), Query Frame = 1
Query:   49 IMGRLVPFNGFVGLEFREVTAEHVQLELPHRPEHIGDPTRPALHGGALAMLADTAGGTLVLAATEAGDKVSTLDMRIDYLRPADLTTTFADATLVRLGRRAAVVRIHVWQPSDTEPDGKRHIADSTAVYSVYRA 450
            I+ R +PF+ F+GL+  E+   + +L +P+RPE IGDP RPALHGG ++MLADT  G+ V AA    D+V+T+DMR+DYLRPAD     A   +  LG R     + V+  S T+PD    IA+   VY++ +A
Sbjct:    6 IIEREIPFDRFLGLQVEEIRPGYARLRMPYRPEFIGDPRRPALHGGLISMLADTCAGSAVWAACSVRDRVATIDMRVDYLRPADPADLIAIGEVKLLGNRVGNATVQVY--SATDPDVV--IAEGRGVYNIRKA 135          
BLAST of mRNA_M-pyrifera_M_contig96111.22154.1 vs. uniprot
Match: A0A1M3N742_9DELT (4HBT domain-containing protein n=1 Tax=Myxococcales bacterium 68-20 TaxID=1895795 RepID=A0A1M3N742_9DELT)

HSP 1 Score: 110 bits (275), Expect = 3.450e-28
Identity = 63/132 (47.73%), Postives = 82/132 (62.12%), Query Frame = 1
Query:   49 IMGRLVPFNGFVGLEFREVTAEHVQLELPHRPEHIGDPTRPALHGGALAMLADTAGGTLVLAATEAGD-KVSTLDMRIDYLRPADLTTTFADATLVRLGRRAAVVRIHVWQPSDTEPDGKRHIADSTAVYSV 441
            +M  L+PFN ++G+   +  A   +LE+P R E IGDP RPALHGG L+ LAD AGG  V A  E    +VST+D+RIDYLRPA L    A+A +VRLG R  V  + ++QPS  E D    IA    VY++
Sbjct:    9 VMEELIPFNRYLGVRMAKAGAGFARLEVPFREELIGDPLRPALHGGVLSALADAAGGAAVWAGLEDDRARVSTIDLRIDYLRPAKLELVVAEAQVVRLGNRVGVADVRLFQPS-AESDT---IATGKGVYNI 136          
BLAST of mRNA_M-pyrifera_M_contig96111.22154.1 vs. uniprot
Match: A0A1G9L753_9DELT (Uncharacterized domain 1-containing protein n=1 Tax=Maridesulfovibrio ferrireducens TaxID=246191 RepID=A0A1G9L753_9DELT)

HSP 1 Score: 110 bits (275), Expect = 3.550e-28
Identity = 60/126 (47.62%), Postives = 82/126 (65.08%), Query Frame = 1
Query:   64 VPFNGFVGLEFREVTAEHVQLELPHRPEHIGDPTRPALHGGALAMLADTAGGTLVLAATEAGDKVSTLDMRIDYLRPADLTTTFADATLVRLGRRAAVVRIHVWQPSDTEPDGKRHIADSTAVYSV 441
            +PF+ F+GL+   +   + +L LP+RPE IGDP RPALHGG ++ML DT GGT V A+ +  D+VST+DMR+DYLRPA      A+A +  LG R    +I V+  SD  PD    +A+  AVY+V
Sbjct:   14 IPFDLFLGLKVEHLEHGYAKLRLPYRPEFIGDPRRPALHGGVISMLIDTCGGTAVWASGDVRDRVSTIDMRVDYLRPAGPEDLIAEARVRLLGNRVGNSQITVYSASD--PD--LIVAEGRAVYNV 135          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig96111.22154.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2E7MAX7_9DELT1.400e-3749.30Thioesterase n=1 Tax=Deltaproteobacteria bacterium... [more]
A0A2E6EKQ5_9RICK5.790e-3652.14Thioesterase n=1 Tax=Rickettsiales bacterium TaxID... [more]
A0A356KLU1_9BACT1.810e-3345.38Thioesterase n=1 Tax=Planctomycetes bacterium TaxI... [more]
A0A0F6YKF8_9DELT2.050e-3145.33Phenylacetic acid degradation-related protein n=2 ... [more]
A0A3M1TR86_9BACT4.240e-3147.66Hotdog fold thioesterase n=1 Tax=Planctomycetes ba... [more]
A0A2M8B8M8_9DELT1.070e-3043.18Thioesterase n=2 Tax=unclassified Deltaproteobacte... [more]
A0A521TTS0_9DELT2.000e-2939.74Hotdog fold thioesterase n=1 Tax=Myxococcaceae bac... [more]
A0A847UXX3_9DELT2.750e-2944.78PaaI family thioesterase n=1 Tax=Desulfovibrionale... [more]
A0A1M3N742_9DELT3.450e-2847.734HBT domain-containing protein n=1 Tax=Myxococcale... [more]
A0A1G9L753_9DELT3.550e-2847.62Uncharacterized domain 1-containing protein n=1 Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig96111contigM-pyrifera_M_contig96111:53..508 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score108.6
Seed ortholog evalue2.6e-21
Seed eggNOG ortholog525897.Dbac_3340
Preferred nameyigI
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0044424,GO:0044444,GO:0044464
EggNOG free text desc.Thioesterase superfamily
EggNOG OGs1RHK6@1224,2MH3Q@213115,2X6J9@28221,43B5Q@68525,COG2050@1,COG2050@2
COG Functional cat.Q
Best tax levelDesulfovibrionales
Best eggNOG OGNA|NA|NA
Exons1
Model size456
Cds size456
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816876.409085-CDS-M-pyrifera_M_contig96111:52..5081622816876.409085-CDS-M-pyrifera_M_contig96111:52..508Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig96111 53..508 +
1692278230.895237-CDS-M-pyrifera_M_contig96111:52..5081692278230.895237-CDS-M-pyrifera_M_contig96111:52..508Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig96111 53..508 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig96111.22154.1prot_M-pyrifera_M_contig96111.22154.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig96111 53..508 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig96111.22154.1

>prot_M-pyrifera_M_contig96111.22154.1 ID=prot_M-pyrifera_M_contig96111.22154.1|Name=mRNA_M-pyrifera_M_contig96111.22154.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=152bp
DEVERARTDRVFAAELIMGRLVPFNGFVGLEFREVTAEHVQLELPHRPEH
IGDPTRPALHGGALAMLADTAGGTLVLAATEAGDKVSTLDMRIDYLRPAD
LTTTFADATLVRLGRRAAVVRIHVWQPSDTEPDGKRHIADSTAVYSVYRA
E*
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mRNA from alignment at M-pyrifera_M_contig96111:53..508+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig96111.22154.1 ID=mRNA_M-pyrifera_M_contig96111.22154.1|Name=mRNA_M-pyrifera_M_contig96111.22154.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=456bp|location=Sequence derived from alignment at M-pyrifera_M_contig96111:53..508+ (Macrocystis pyrifera P11B4 male)
GACGAGGTCGAGCGCGCGCGCACGGACCGCGTCTTCGCCGCCGAACTGAT CATGGGCCGGCTCGTGCCGTTCAACGGCTTCGTCGGGCTCGAGTTCCGGG AAGTCACGGCCGAGCACGTCCAGCTCGAGCTGCCCCACCGCCCCGAGCAC ATCGGCGACCCGACGCGCCCCGCCCTGCACGGCGGCGCACTCGCGATGCT GGCCGACACCGCGGGCGGCACGCTCGTCCTCGCCGCGACCGAAGCCGGCG ACAAGGTCTCCACCCTCGACATGCGCATCGACTACTTGCGCCCCGCCGAC CTCACGACCACCTTCGCGGACGCGACCCTCGTCCGCCTGGGCCGCCGCGC CGCCGTCGTCCGCATCCACGTCTGGCAACCCAGCGACACCGAGCCCGACG GCAAACGCCACATCGCCGACTCGACGGCGGTCTACTCGGTCTACCGCGCG GAATAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig96111:53..508+

>mRNA_M-pyrifera_M_contig96111.22154.1 ID=mRNA_M-pyrifera_M_contig96111.22154.1|Name=mRNA_M-pyrifera_M_contig96111.22154.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=912bp|location=Sequence derived from alignment at M-pyrifera_M_contig96111:53..508+ (Macrocystis pyrifera P11B4 male)
GACGAGGTCGAGCGCGCGCGCACGGACCGCGTCTTCGCCGCCGAACTGAT
CATGGGCCGGCTCGTGCCGTTCAACGGCTTCGTCGGGCTCGAGTTCCGGG
AAGTCACGGCCGAGCACGTCCAGCTCGAGCTGCCCCACCGCCCCGAGCAC
ATCGGCGACCCGACGCGCCCCGCCCTGCACGGCGGCGCACTCGCGATGCT
GGCCGACACCGCGGGCGGCACGCTCGTCCTCGCCGCGACCGAAGCCGGCG
ACAAGGTCTCCACCCTCGACATGCGCATCGACTACTTGCGCCCCGCCGAC
CTCACGACCACCTTCGCGGACGCGACCCTCGTCCGCCTGGGCCGCCGCGC
CGCCGTCGTCCGCATCCACGTCTGGCAACCCAGCGACACCGAGCCCGACG
GCAAACGCCACATCGCCGACTCGACGGCGGTCTACTCGGTCTACCGCGCG
GAATAGGACGAGGTCGAGCGCGCGCGCACGGACCGCGTCTTCGCCGCCGA
ACTGATCATGGGCCGGCTCGTGCCGTTCAACGGCTTCGTCGGGCTCGAGT
TCCGGGAAGTCACGGCCGAGCACGTCCAGCTCGAGCTGCCCCACCGCCCC
GAGCACATCGGCGACCCGACGCGCCCCGCCCTGCACGGCGGCGCACTCGC
GATGCTGGCCGACACCGCGGGCGGCACGCTCGTCCTCGCCGCGACCGAAG
CCGGCGACAAGGTCTCCACCCTCGACATGCGCATCGACTACTTGCGCCCC
GCCGACCTCACGACCACCTTCGCGGACGCGACCCTCGTCCGCCTGGGCCG
CCGCGCCGCCGTCGTCCGCATCCACGTCTGGCAACCCAGCGACACCGAGC
CCGACGGCAAACGCCACATCGCCGACTCGACGGCGGTCTACTCGGTCTAC
CGCGCGGAATAG
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