mRNA_M-pyrifera_M_contig96109.22153.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig96109.22153.1
Unique NamemRNA_M-pyrifera_M_contig96109.22153.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig96109.22153.1 vs. uniprot
Match: A0A667YB10_9TELE (Protein DENND6A-like n=1 Tax=Myripristis murdjan TaxID=586833 RepID=A0A667YB10_9TELE)

HSP 1 Score: 128 bits (322), Expect = 9.610e-33
Identity = 68/162 (41.98%), Postives = 95/162 (58.64%), Query Frame = 1
Query:   22 LTSVKQLTYGGDFRPFFTIHDTDFKKYTNPDDEPRAVIIGATNPFFFKAMDHWPHIVTIDAQKSTNERGSVGKGMASLVKLPGAKKRSTSGNFVNFKQEVKSAYRTHMAPDPQLLKNLVHKKSQKVTNAVNNELLRRHFVARTEMFLVPLERYFTSLMPLQK 507
            ++ +  L Y  DFRP+FTIHD++FK+YT     P +VI+G TNPFF K + HWPHI+ I   K   E       MA  +K+   K   T    ++ K  V SAY+ ++  D  ++K L     QK  +A  N +LRR+F+  T+ F++PLERY  SLMPLQK
Sbjct:   77 VSCISPLRYCSDFRPYFTIHDSEFKEYTTRTQAPPSVILGVTNPFFAKTLQHWPHIIRIGDMKQAGE-------MAKQMKVKKLKNLKT----LDSKPGVYSAYKPYLNKDEDIIKQLQKGVQQKRPSAAQNAILRRYFLELTQSFIIPLERYVASLMPLQK 227          
BLAST of mRNA_M-pyrifera_M_contig96109.22153.1 vs. uniprot
Match: A0A8C4BS00_9TELE (DENN domain containing 6A n=1 Tax=Denticeps clupeoides TaxID=299321 RepID=A0A8C4BS00_9TELE)

HSP 1 Score: 128 bits (322), Expect = 1.180e-32
Identity = 69/162 (42.59%), Postives = 95/162 (58.64%), Query Frame = 1
Query:   22 LTSVKQLTYGGDFRPFFTIHDTDFKKYTNPDDEPRAVIIGATNPFFFKAMDHWPHIVTIDAQKSTNERGSVGKGMASLVKLPGAKKRSTSGNFVNFKQEVKSAYRTHMAPDPQLLKNLVHKKSQKVTNAVNNELLRRHFVARTEMFLVPLERYFTSLMPLQK 507
            ++ +  L Y  DFRP+FTIHD++FK+YT     P +VI+G TNPFF K + HWPHI+ I   K T E       MA  +K+   K   T    ++ K  V SAY+  +  D  ++K L     QK  +A  N +LRR+F+  T+ F++PLERY  SLMPLQK
Sbjct:   69 VSCISPLRYCSDFRPYFTIHDSEFKEYTTRTQAPPSVILGVTNPFFAKTLQHWPHIIRIGDMKQTGE-------MAKHMKVKKLKNLKT----LDSKPGVYSAYKPFLNKDEDIIKQLQKGVHQKRPSAAQNAILRRYFLELTQSFIIPLERYVASLMPLQK 219          
BLAST of mRNA_M-pyrifera_M_contig96109.22153.1 vs. uniprot
Match: A0A8D0ATF0_SANLU (DENN/MADD domain containing 6Aa n=1 Tax=Sander lucioperca TaxID=283035 RepID=A0A8D0ATF0_SANLU)

HSP 1 Score: 128 bits (321), Expect = 1.510e-32
Identity = 67/162 (41.36%), Postives = 96/162 (59.26%), Query Frame = 1
Query:   22 LTSVKQLTYGGDFRPFFTIHDTDFKKYTNPDDEPRAVIIGATNPFFFKAMDHWPHIVTIDAQKSTNERGSVGKGMASLVKLPGAKKRSTSGNFVNFKQEVKSAYRTHMAPDPQLLKNLVHKKSQKVTNAVNNELLRRHFVARTEMFLVPLERYFTSLMPLQK 507
            ++ +  L Y  DFRP+FTIHD++FK+YT     P +VI+G TNPFF K + HWPHI+ I   K   E       MA  +K+   K   T    ++ K  V +AY+ ++  D +++K L     QK  +A  N +LRR+F+  T+ F++PLERY  SLMPLQK
Sbjct:   64 VSCISPLRYCSDFRPYFTIHDSEFKEYTTRTQAPPSVILGVTNPFFAKTLQHWPHIIRIGDMKQAGE-------MAKQMKVKKLKNLKT----LDSKPGVYTAYKPYLNKDEEIIKQLQKGVQQKRPSAAQNAILRRYFLELTQSFIIPLERYVASLMPLQK 214          
BLAST of mRNA_M-pyrifera_M_contig96109.22153.1 vs. uniprot
Match: A0A8C4DM21_DICLA (DENN/MADD domain containing 6Aa n=1 Tax=Dicentrarchus labrax TaxID=13489 RepID=A0A8C4DM21_DICLA)

HSP 1 Score: 128 bits (321), Expect = 1.740e-32
Identity = 67/162 (41.36%), Postives = 96/162 (59.26%), Query Frame = 1
Query:   22 LTSVKQLTYGGDFRPFFTIHDTDFKKYTNPDDEPRAVIIGATNPFFFKAMDHWPHIVTIDAQKSTNERGSVGKGMASLVKLPGAKKRSTSGNFVNFKQEVKSAYRTHMAPDPQLLKNLVHKKSQKVTNAVNNELLRRHFVARTEMFLVPLERYFTSLMPLQK 507
            ++ +  L Y  DFRP+FTIHD++FK+YT     P +VI+G TNPFF K + HWPHI+ I   K   E       MA  +K+   K   T    ++ K  V +AY+ ++  D +++K L     QK  +A  N +LRR+F+  T+ F++PLERY  SLMPLQK
Sbjct:   72 VSCISPLRYCSDFRPYFTIHDSEFKEYTTRTQAPPSVILGVTNPFFAKTLQHWPHIIRIGDMKQAGE-------MAKQMKVKKLKNLKT----LDSKPGVYTAYKPYLNKDEEIIKQLQKGVQQKRPSAAQNAILRRYFLELTQSFIIPLERYVASLMPLQK 222          
BLAST of mRNA_M-pyrifera_M_contig96109.22153.1 vs. uniprot
Match: A0A669C751_ORENI (UDENN domain-containing protein n=4 Tax=Percomorphaceae TaxID=1489872 RepID=A0A669C751_ORENI)

HSP 1 Score: 127 bits (320), Expect = 2.850e-32
Identity = 67/162 (41.36%), Postives = 96/162 (59.26%), Query Frame = 1
Query:   22 LTSVKQLTYGGDFRPFFTIHDTDFKKYTNPDDEPRAVIIGATNPFFFKAMDHWPHIVTIDAQKSTNERGSVGKGMASLVKLPGAKKRSTSGNFVNFKQEVKSAYRTHMAPDPQLLKNLVHKKSQKVTNAVNNELLRRHFVARTEMFLVPLERYFTSLMPLQK 507
            ++ +  L Y  DFRP+FTIHD++FK+YT     P +VI+G TNPFF K + HWPHI+ I   K   E       MA  +K+   K   T    ++ K  V +AY+ ++  D +++K L     QK  +A  N +LRR+F+  T+ F++PLERY  SLMPLQK
Sbjct:   81 VSCIAPLRYCSDFRPYFTIHDSEFKEYTTRTQAPPSVILGVTNPFFAKTLQHWPHIIRIGDMKQAGE-------MAKQMKVKKLKNLKT----LDSKPGVYTAYKPYLNKDEEIIKQLQKGVQQKRPSAAQNAILRRYFLELTQSFIIPLERYVASLMPLQK 231          
BLAST of mRNA_M-pyrifera_M_contig96109.22153.1 vs. uniprot
Match: UPI001C368900 ((large yellow croaker) hypothetical protein n=1 Tax=Larimichthys crocea TaxID=215358 RepID=UPI001C368900)

HSP 1 Score: 128 bits (321), Expect = 3.330e-32
Identity = 67/162 (41.36%), Postives = 96/162 (59.26%), Query Frame = 1
Query:   22 LTSVKQLTYGGDFRPFFTIHDTDFKKYTNPDDEPRAVIIGATNPFFFKAMDHWPHIVTIDAQKSTNERGSVGKGMASLVKLPGAKKRSTSGNFVNFKQEVKSAYRTHMAPDPQLLKNLVHKKSQKVTNAVNNELLRRHFVARTEMFLVPLERYFTSLMPLQK 507
            ++ +  L Y  DFRP+FTIHD++FK+YT     P +VI+G TNPFF K + HWPHI+ I   K   E       MA  +K+   K   T    ++ K  V +AY+ ++  D +++K L     QK  +A  N +LRR+F+  T+ F++PLERY  SLMPLQK
Sbjct:  111 VSCISPLRYCSDFRPYFTIHDSEFKEYTTRTQAPPSVILGVTNPFFAKTLQHWPHIIRIGDMKQAGE-------MAKQMKVKKLKNLKT----LDSKPGVYTAYKPYLNKDEEIIKQLQKGVQQKRPSAAQNAILRRYFLELTQSFIIPLERYVASLMPLQK 261          
BLAST of mRNA_M-pyrifera_M_contig96109.22153.1 vs. uniprot
Match: A0A553RPU1_9TELE (UDENN domain-containing protein n=1 Tax=Danionella translucida TaxID=623744 RepID=A0A553RPU1_9TELE)

HSP 1 Score: 125 bits (313), Expect = 3.620e-32
Identity = 67/159 (42.14%), Postives = 92/159 (57.86%), Query Frame = 1
Query:   31 VKQLTYGGDFRPFFTIHDTDFKKYTNPDDEPRAVIIGATNPFFFKAMDHWPHIVTIDAQKSTNERGSVGKGMASLVKLPGAKKRSTSGNFVNFKQEVKSAYRTHMAPDPQLLKNLVHKKSQKVTNAVNNELLRRHFVARTEMFLVPLERYFTSLMPLQK 507
            +  L Y  DFRP+FTIHD++FK+YT     P +VI+G TNPFF K + HWPHI+ I   K   E       MA  +K+   K   T    ++ K  V +AY+  +  D  ++K L     QK  +A  N +LRR+F+  T+ F++PLERY  SLMPLQK
Sbjct:   10 IAPLRYCSDFRPYFTIHDSEFKEYTTRTQAPPSVILGVTNPFFAKTLQHWPHIIRIGDMKQPGE-------MAKQMKVKKLKNLKT----LDSKPGVYTAYKPFLNKDEDIIKQLQKGVQQKRPSAAQNAILRRYFLELTQSFIIPLERYVASLMPLQK 157          
BLAST of mRNA_M-pyrifera_M_contig96109.22153.1 vs. uniprot
Match: H2MSI1_ORYLA (DENN domain containing 6A n=1 Tax=Oryzias latipes TaxID=8090 RepID=H2MSI1_ORYLA)

HSP 1 Score: 130 bits (326), Expect = 4.880e-32
Identity = 68/162 (41.98%), Postives = 98/162 (60.49%), Query Frame = 1
Query:   22 LTSVKQLTYGGDFRPFFTIHDTDFKKYTNPDDEPRAVIIGATNPFFFKAMDHWPHIVTIDAQKSTNERGSVGKGMASLVKLPGAKKRSTSGNFVNFKQEVKSAYRTHMAPDPQLLKNLVHKKSQKVTNAVNNELLRRHFVARTEMFLVPLERYFTSLMPLQK 507
            ++ +  L Y  DFRP+FTIHD++FK+YT     P +VI+G TNPFF K + HWPHI+ I   K T ER +  K    + KL   K        ++ K  V +AY+ +++ D +++K L     QK  +A  N +LRR+F+  T+ F++PLERY  SLMPLQK
Sbjct:  309 VSCISPLRYCSDFRPYFTIHDSEFKEYTTRTQAPPSVILGVTNPFFAKTLQHWPHIIRIGDMKQTGERPAQMK----VKKLKNLK-------TLDSKPGVYTAYKPYLSKDEEIIKQLQKGVQQKRPSAAQNAILRRYFLELTQSFIIPLERYVASLMPLQK 459          
BLAST of mRNA_M-pyrifera_M_contig96109.22153.1 vs. uniprot
Match: UPI000E45F266 (protein DENND6A isoform X2 n=1 Tax=Mastacembelus armatus TaxID=205130 RepID=UPI000E45F266)

HSP 1 Score: 129 bits (324), Expect = 5.040e-32
Identity = 68/162 (41.98%), Postives = 97/162 (59.88%), Query Frame = 1
Query:   22 LTSVKQLTYGGDFRPFFTIHDTDFKKYTNPDDEPRAVIIGATNPFFFKAMDHWPHIVTIDAQKSTNERGSVGKGMASLVKLPGAKKRSTSGNFVNFKQEVKSAYRTHMAPDPQLLKNLVHKKSQKVTNAVNNELLRRHFVARTEMFLVPLERYFTSLMPLQK 507
            ++ +  L Y  DFRP+FTIHD++FK+YT     P +VI+G TNPFF K + HWPHI+ I   K T E       MA  +K+   K   T    ++ K  V +AY+ ++  D +++K L     QK  +A  N +LRR+F+  T+ F++PLERY  SLMPLQK
Sbjct:  310 VSCISPLRYCTDFRPYFTIHDSEFKEYTTRTQAPPSVILGVTNPFFAKTLQHWPHIIRIGDMKQTGE-------MAKQMKVKKLKNLKT----LDSKPGVYTAYKPYLNKDEEIIKQLQKGVQQKRPSAAQNAILRRYFLELTQSFIIPLERYVASLMPLQK 460          
BLAST of mRNA_M-pyrifera_M_contig96109.22153.1 vs. uniprot
Match: A0A4Y7M2W2_9CRUS (EOG090X031T n=1 Tax=Daphnia barbata TaxID=414587 RepID=A0A4Y7M2W2_9CRUS)

HSP 1 Score: 129 bits (323), Expect = 5.110e-32
Identity = 70/170 (41.18%), Postives = 98/170 (57.65%), Query Frame = 1
Query:    1 SHIFCWSLTSVKQLTYGGDFRPFFTIHDTDFKKYTN-PDDEPRAVIIGATNPFFFKAMDHWPHIVTIDAQKSTNERGSVGKGMASLVKLPGAKKRSTSGNFVNFKQEVKSAYRTHMAPDPQLLKNLVHKKSQKVTNAVNNELLRRHFVARTEMFLVPLERYFTSLMPLQK 507
            SH+    +  +  L Y  D+RPFFTIHD++FK+YT    D P +V++G TNPFF KA+ HWPHI+ I A+  +N  G   +   S V        S S      K  V ++Y++H+  D  LLK +      +  N V + L+RRHF+  T+ F++PLERYF  LMPLQK
Sbjct:  133 SHVVQALVNLIHPLQYCLDYRPFFTIHDSEFKEYTGRSSDAPPSVLLGVTNPFFAKALQHWPHIIRIGAEVPSNSGGLKIRATKSNV--------SGSSKMSECKPGVYTSYKSHLTRDKALLKLIAKGTQTRRPNQVQSALMRRHFLELTQSFIIPLERYFARLMPLQK 294          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig96109.22153.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A667YB10_9TELE9.610e-3341.98Protein DENND6A-like n=1 Tax=Myripristis murdjan T... [more]
A0A8C4BS00_9TELE1.180e-3242.59DENN domain containing 6A n=1 Tax=Denticeps clupeo... [more]
A0A8D0ATF0_SANLU1.510e-3241.36DENN/MADD domain containing 6Aa n=1 Tax=Sander luc... [more]
A0A8C4DM21_DICLA1.740e-3241.36DENN/MADD domain containing 6Aa n=1 Tax=Dicentrarc... [more]
A0A669C751_ORENI2.850e-3241.36UDENN domain-containing protein n=4 Tax=Percomorph... [more]
UPI001C3689003.330e-3241.36(large yellow croaker) hypothetical protein n=1 Ta... [more]
A0A553RPU1_9TELE3.620e-3242.14UDENN domain-containing protein n=1 Tax=Danionella... [more]
H2MSI1_ORYLA4.880e-3241.98DENN domain containing 6A n=1 Tax=Oryzias latipes ... [more]
UPI000E45F2665.040e-3241.98protein DENND6A isoform X2 n=1 Tax=Mastacembelus a... [more]
A0A4Y7M2W2_9CRUS5.110e-3241.18EOG090X031T n=1 Tax=Daphnia barbata TaxID=414587 R... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig96109contigM-pyrifera_M_contig96109:193..702 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeArthropoda
Seed ortholog score133.3
Seed ortholog evalue1.1e-28
Seed eggNOG ortholog6669.EFX89439
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0005085,GO:0005088,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005768,GO:0008150,GO:0012505,GO:0017016,GO:0017112,GO:0017137,GO:0019899,GO:0031267,GO:0031410,GO:0031982,GO:0043085,GO:0043087,GO:0043226,GO:0043227,GO:0043229,GO:0043547,GO:0044093,GO:0044424,GO:0044444,GO:0044464,GO:0050790,GO:0051020,GO:0051336,GO:0051345,GO:0055037,GO:0065007,GO:0065009,GO:0097708,GO:0098772
EggNOG free text desc.Stabilization of polarity axis
EggNOG OGs38F00@33154,3BD28@33208,3CVK1@33213,41YBN@6656,KOG2432@1,KOG2432@2759
COG Functional cat.O
Best tax levelArthropoda
Best eggNOG OGNA|NA|NA
Exons1
Model size510
Cds size510
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816876.3691916-CDS-M-pyrifera_M_contig96109:192..7021622816876.3691916-CDS-M-pyrifera_M_contig96109:192..702Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig96109 193..702 -
1692278230.8774838-CDS-M-pyrifera_M_contig96109:192..7021692278230.8774838-CDS-M-pyrifera_M_contig96109:192..702Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig96109 193..702 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig96109.22153.1prot_M-pyrifera_M_contig96109.22153.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig96109 193..702 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig96109.22153.1

>prot_M-pyrifera_M_contig96109.22153.1 ID=prot_M-pyrifera_M_contig96109.22153.1|Name=mRNA_M-pyrifera_M_contig96109.22153.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=170bp
SHIFCWSLTSVKQLTYGGDFRPFFTIHDTDFKKYTNPDDEPRAVIIGATN
PFFFKAMDHWPHIVTIDAQKSTNERGSVGKGMASLVKLPGAKKRSTSGNF
VNFKQEVKSAYRTHMAPDPQLLKNLVHKKSQKVTNAVNNELLRRHFVART
EMFLVPLERYFTSLMPLQK*
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mRNA from alignment at M-pyrifera_M_contig96109:193..702-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig96109.22153.1 ID=mRNA_M-pyrifera_M_contig96109.22153.1|Name=mRNA_M-pyrifera_M_contig96109.22153.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=510bp|location=Sequence derived from alignment at M-pyrifera_M_contig96109:193..702- (Macrocystis pyrifera P11B4 male)
TCACATATCTTCTGCTGGTCACTGACCTCCGTGAAACAGTTGACGTACGG CGGAGACTTTCGGCCGTTCTTCACGATTCACGACACAGATTTCAAGAAGT ATACAAATCCAGACGATGAGCCACGTGCAGTGATAATCGGAGCGACGAAT CCCTTCTTCTTCAAGGCCATGGACCACTGGCCTCACATTGTGACAATTGA TGCACAGAAGTCAACAAATGAGCGAGGCTCCGTGGGCAAGGGAATGGCCT CTCTCGTCAAGTTGCCAGGCGCCAAGAAGAGGTCCACCAGCGGTAACTTT GTGAACTTCAAGCAGGAGGTGAAGAGTGCCTATCGCACGCACATGGCCCC AGACCCGCAGCTGCTGAAGAACCTCGTGCACAAGAAGAGCCAGAAGGTCA CCAACGCCGTTAACAACGAACTTCTGCGCCGCCACTTCGTCGCACGCACA GAAATGTTCCTCGTGCCGCTCGAGCGCTATTTCACCTCGCTCATGCCTCT CCAGAAGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig96109:193..702-

>mRNA_M-pyrifera_M_contig96109.22153.1 ID=mRNA_M-pyrifera_M_contig96109.22153.1|Name=mRNA_M-pyrifera_M_contig96109.22153.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1020bp|location=Sequence derived from alignment at M-pyrifera_M_contig96109:193..702- (Macrocystis pyrifera P11B4 male)
TCACATATCTTCTGCTGGTCACTGACCTCCGTGAAACAGTTGACGTACGG
CGGAGACTTTCGGCCGTTCTTCACGATTCACGACACAGATTTCAAGAAGT
ATACAAATCCAGACGATGAGCCACGTGCAGTGATAATCGGAGCGACGAAT
CCCTTCTTCTTCAAGGCCATGGACCACTGGCCTCACATTGTGACAATTGA
TGCACAGAAGTCAACAAATGAGCGAGGCTCCGTGGGCAAGGGAATGGCCT
CTCTCGTCAAGTTGCCAGGCGCCAAGAAGAGGTCCACCAGCGGTAACTTT
GTGAACTTCAAGCAGGAGGTGAAGAGTGCCTATCGCACGCACATGGCCCC
AGACCCGCAGCTGCTGAAGAACCTCGTGCACAAGAAGAGCCAGAAGGTCA
CCAACGCCGTTAACAACGAACTTCTGCGCCGCCACTTCGTCGCACGCACA
GAAATGTTCCTCGTGCCGCTCGAGCGCTATTTCACCTCGCTCATGCCTCT
CCAGAAGTAATCACATATCTTCTGCTGGTCACTGACCTCCGTGAAACAGT
TGACGTACGGCGGAGACTTTCGGCCGTTCTTCACGATTCACGACACAGAT
TTCAAGAAGTATACAAATCCAGACGATGAGCCACGTGCAGTGATAATCGG
AGCGACGAATCCCTTCTTCTTCAAGGCCATGGACCACTGGCCTCACATTG
TGACAATTGATGCACAGAAGTCAACAAATGAGCGAGGCTCCGTGGGCAAG
GGAATGGCCTCTCTCGTCAAGTTGCCAGGCGCCAAGAAGAGGTCCACCAG
CGGTAACTTTGTGAACTTCAAGCAGGAGGTGAAGAGTGCCTATCGCACGC
ACATGGCCCCAGACCCGCAGCTGCTGAAGAACCTCGTGCACAAGAAGAGC
CAGAAGGTCACCAACGCCGTTAACAACGAACTTCTGCGCCGCCACTTCGT
CGCACGCACAGAAATGTTCCTCGTGCCGCTCGAGCGCTATTTCACCTCGC
TCATGCCTCTCCAGAAGTAA
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