mRNA_M-pyrifera_M_contig95708.22070.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig95708.22070.1
Unique NamemRNA_M-pyrifera_M_contig95708.22070.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig95708.22070.1 vs. uniprot
Match: L8HC75_ACACA (C2 domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8HC75_ACACA)

HSP 1 Score: 81.3 bits (199), Expect = 2.410e-15
Identity = 50/139 (35.97%), Postives = 74/139 (53.24%), Query Frame = 1
Query:    1 PPVEELLCVYGKNLPTEVGYIYRPFKKTKFNTFGEPLTLKLDRSRSKAKRVLEGTKGFLAKHGIIFEVPDRDEDIQS----------GDGTVPYASLAYHKKWENTLGAPTITSVEIEGADDREMLSMPLVIGSLVRHI 387
            PPV+ L  +YG NL TE+GY++      K +  G    + LD S SKA+R    ++GF  K GI++E P   + I            GDGTV YASL +  +W N +  P +   E+EGA+ R++L+  L    L+ +I
Sbjct:  870 PPVDRLYAIYGVNLETEIGYVF------KRDAAG---CIVLDDSISKAQRTQLESEGFAVKKGIVWETPRTKQGIVRELTGVDAHICGDGTVTYASLNHCARWRNEI--PKLKIEELEGAEHRQVLANRLFFKKLIEYI 997          
BLAST of mRNA_M-pyrifera_M_contig95708.22070.1 vs. uniprot
Match: L8GQ15_ACACA (C2 domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GQ15_ACACA)

HSP 1 Score: 74.3 bits (181), Expect = 6.360e-13
Identity = 46/139 (33.09%), Postives = 72/139 (51.80%), Query Frame = 1
Query:    1 PPVEELLCVYGKNLPTEVGYIYRPFKKTKFNTFGEPLTLKLDRSRSKAKRVLEGTKGFLAKHGIIFEVPDRDEDI----------QSGDGTVPYASLAYHKKWENTLGAPTITSVEIEGADDREMLSMPLVIGSLVRHI 387
            PPV  L  +YG NL TE+GY+   FK+ K  +      L LD +   +K  L+   GF+ K GI++E     + I            GDGTV Y+SL +  +W N +  P +T  E++GA+ RE+L+  L    ++ ++
Sbjct:  775 PPVRRLYAIYGINLDTEIGYV---FKREKSGS------LALDDNSPPSKNGLQND-GFVFKKGIVYETSKSKQGIVREMTGVDGFTCGDGTVSYSSLNHCARWRNDI--PQLTIEELDGAEHREVLANRLFFKKMIEYV 901          
BLAST of mRNA_M-pyrifera_M_contig95708.22070.1 vs. uniprot
Match: A0A7S4L5L0_9EUKA (Hypothetical protein n=2 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4L5L0_9EUKA)

HSP 1 Score: 68.2 bits (165), Expect = 8.200e-11
Identity = 44/137 (32.12%), Postives = 65/137 (47.45%), Query Frame = 1
Query:    4 PVEELLCVYGKNLPTEVGYIYRPFKKTKFNTFGEPLTLKLDRSRSKAKRVLEGTKGFLAKHGIIFEVPDRDEDIQ------SGDGTVPYASLAYHKKWENTLGAP----TITSVEIEGADDREMLSMPLVIGSLVRH 384
            P++ L CVYG N  TEVGY Y+  ++ KF        L  +   S  KR     K    + GI +E     +         SGDGTVP+ASLAY  +WE           +   EI+G++ R+ML    V+ +++ +
Sbjct:  311 PIKNLFCVYGVNRATEVGYYYKRARQGKF-------VLDTEADVSSKKRTEINPKALTIRGGIAYETRTTVQTAAGVKKAVSGDGTVPFASLAYPLEWEKMAKERGVDFNLEITEIQGSEHRKMLQDESVLINILSY 440          
BLAST of mRNA_M-pyrifera_M_contig95708.22070.1 vs. uniprot
Match: A0A7S4V0Q8_9EUKA (Hypothetical protein n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4V0Q8_9EUKA)

HSP 1 Score: 65.1 bits (157), Expect = 5.750e-10
Identity = 44/137 (32.12%), Postives = 65/137 (47.45%), Query Frame = 1
Query:    1 PPVEELLCVYGKNLPTEVGYIYRPFKKTKFNTFGEPLTLKLDRSRSKAKRVLEGTKGFLAKHGIIFEV-----PDRDEDIQSGDGTVPYASLAYHKKWENTLGAPTITS----VEIEGADDREMLSMPLVIGSLVRH 384
            PP++ + C+YG N  TEVGY Y+  K  K+      L    D S  K+  +         + GI +E      P     + SGDGTVPY SLAY  +WE       I       EI+G++ R+ML    V+ +++ +
Sbjct:  135 PPIKNMFCLYGVNRATEVGYYYKQNKSGKYF-----LDSSADESSKKSDAI--NPNKLTIRGGIAYETRTTVQPASAGVMISGDGTVPYQSLAYPLQWEKMAKERGIDFNLEITEIQGSEHRKMLQEESVLLNVLSY 264          
BLAST of mRNA_M-pyrifera_M_contig95708.22070.1 vs. uniprot
Match: A0A7S4IHK8_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4IHK8_9EUKA)

HSP 1 Score: 59.7 bits (143), Expect = 1.580e-8
Identity = 43/135 (31.85%), Postives = 58/135 (42.96%), Query Frame = 1
Query:    1 PPVEELLCVYGKNLPTEVGYIYRPFKKTKFNTFGEPLTLKLDRSRSKAKRVLEGTK-GFLAKHGIIFEVPDRDED----IQSGDGTVPYASLAYHKKW----ENTLGAPTITSVEIEGADDREMLSMPLVIGSLV 378
            PPV  L C+YG NL TE+ Y Y        N+      L  + S        E  K G     G+ FE  D  ++    + SGDGTVP+ SLAY  +W    E       I   E+   + R ML   +VI  ++
Sbjct:   29 PPVANLWCIYGINLKTEISYYY--------NSHDSHYHLDSNASALNGTVQEEVNKHGLPVSSGVAFETKDTPQEAFGRVGSGDGTVPFCSLAYCHQWKARAEEKKTGQNIVIHELHQKEHRTMLKDDVVIDKIL 155          
BLAST of mRNA_M-pyrifera_M_contig95708.22070.1 vs. uniprot
Match: A0A7S4MHF2_9EUKA (Hypothetical protein n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4MHF2_9EUKA)

HSP 1 Score: 59.7 bits (143), Expect = 8.060e-8
Identity = 41/134 (30.60%), Postives = 58/134 (43.28%), Query Frame = 1
Query:    1 PPVEELLCVYGKNLPTEVGYIYRPFKKTKFNTFGEPLTLKLDRSRSKAKRVLEGTKGFLAKHGIIFEVPDRDED----IQSGDGTVPYASLAYHKKW----ENTLGAPTITSVEIEGADDREMLSMPLVIGSLV 378
            PPV  L C+YG NL TE+ Y Y   K  + +     L   +D + +K         G     G+ FE     ++    + SGDGTVP+ SLAY   W    E       I   E+   + R ML   +VI  ++
Sbjct:  560 PPVSNLWCIYGINLKTEISYFYDENKNFQLDGAASILNETVDETTNK--------HGLPIISGVGFETKTTPQEAYGRVGSGDGTVPFCSLAYCHVWKARAEEKKTGQNIVIHELHQKEHRTMLKDDVVIDKIL 685          
BLAST of mRNA_M-pyrifera_M_contig95708.22070.1 vs. uniprot
Match: A0A7S4JD38_9STRA (Hypothetical protein n=1 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4JD38_9STRA)

HSP 1 Score: 59.3 bits (142), Expect = 1.110e-7
Identity = 48/151 (31.79%), Postives = 71/151 (47.02%), Query Frame = 1
Query:    1 PPVEELLCVYGKNLPTEVGYIYRPFKKTKFNTFGEPLT-LKLDRSRSKAKRVLEGTKGFLAKHGIIFEVPDR-----DEDIQ----------SGDGTVPYASLAYHKKWENTLGAPTITS---------VEIEGADDREMLSMPLVIGSLV 378
            PPV+ +  +YG N+PT +G ++R  ++TK     EP T LKLD      K        F  K+G++ EVP R     D ++           SGDGTV Y SL +   W    GA T  +         +E+EGA  R++L     I +++
Sbjct:  488 PPVKRVWPLYGINVPTLIGSVFR--RQTKRYARDEPSTGLKLD------KHCTVDHPCFEVKNGVVKEVPHRTPQADDPNVPADEMTFGTRASGDGTVAYWSLRWPVAWRGEEGAATAAAAGKIDVDDPLEVEGAGHRDILEKSPCIAAVL 630          
BLAST of mRNA_M-pyrifera_M_contig95708.22070.1 vs. uniprot
Match: A0A7S4JRH4_9EUKA (Hypothetical protein n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4JRH4_9EUKA)

HSP 1 Score: 58.9 bits (141), Expect = 1.170e-7
Identity = 44/141 (31.21%), Postives = 66/141 (46.81%), Query Frame = 1
Query:    1 PPVEELLCVYGKNLPTEVGYIYRPFKKTKFNTFGEPLTLKLDRSRSKAKRV--LEGTKGFLAKHGIIFEVPDRDED------IQSGDGTVPYASLAYHKKW------ENTLGAPTITSVEIEGADDREMLSMPLVIGSLVR 381
            P ++ + CVYG    TE     +  +K      G+   +KLD        V      K  L + GI +E    D+         SGDGTVPYASL++ ++W      ++TL  P +   EI+ A+ REMLS   V+  ++R
Sbjct:  147 PNIDNVFCVYGTQKKTEACLYLKRDEK------GD---VKLDERADIFSGVECAGNPKKLLIQKGIGYETRTTDQSNCQYNVSSSGDGTVPYASLSFPRRWKQLMDGDDTLEGPNVEIFEIKNAEHREMLSDDAVLYHVIR 278          
BLAST of mRNA_M-pyrifera_M_contig95708.22070.1 vs. uniprot
Match: A0A367Q6V7_9NOSO (Uncharacterized protein n=1 Tax=Nostoc sp. ATCC 43529 TaxID=1840705 RepID=A0A367Q6V7_9NOSO)

HSP 1 Score: 58.9 bits (141), Expect = 1.450e-7
Identity = 46/141 (32.62%), Postives = 65/141 (46.10%), Query Frame = 1
Query:    1 PPVEELLCVYGKNLPTEVG-YIYRPFKKTKFNTFGEPLTLKLDRSRSKAKRVLEGTKGFLAKHGIIFEVPDR------DEDI-QSGDGTVPYASLAYHKKWENTLGAP--TITS---VEIEGADDREMLSMPLVIGSLVRH 384
            PPV+ +  +Y     T VG Y Y+   K K  ++       L       + V +G   F+  +G+ FE  D+      D+ I  SGDG VPY SLAY K+W N    P   IT    V+ E  +D   L + L   S++RH
Sbjct:  392 PPVKNIDVIYSTGFNTPVGAYYYQEEGKLKVASY-------LGNETENLQPVEDGD--FVVINGVRFEKADKTTQLISDDGITNSGDGVVPYGSLAYFKRWTNNPDVPGQKITGYPFVKTEPTEDEAKLGIDLSHNSIIRH 523          
BLAST of mRNA_M-pyrifera_M_contig95708.22070.1 vs. uniprot
Match: A0A7S4UEQ9_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4UEQ9_9EUKA)

HSP 1 Score: 57.4 bits (137), Expect = 5.110e-7
Identity = 36/98 (36.73%), Postives = 49/98 (50.00%), Query Frame = 1
Query:    1 PPVEELLCVYGKNLPTEVGYIYRPFKKTKFNTFGEPLTLKLDRSRSKAKRVLEGTKGFLAKHGIIFEVPDRDEDI----QSGDGTVPYASLAYHKKWE 282
            PPV+ L  +YG N+ TEV Y +    K+ +   G  L    D+   K K      KG   + G+ +E  D  + I    +SGDGTVPY SLAY   W+
Sbjct:  526 PPVKRLWAIYGVNVLTEVSYYF----KSNWKKDGIALDSNADKLGGK-KTAKTNPKGLEIQEGVGYEGKDTLQSIGGVQRSGDGTVPYCSLAYGTMWK 618          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig95708.22070.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 11
Match NameE-valueIdentityDescription
L8HC75_ACACA2.410e-1535.97C2 domain containing protein n=1 Tax=Acanthamoeba ... [more]
L8GQ15_ACACA6.360e-1333.09C2 domain containing protein n=1 Tax=Acanthamoeba ... [more]
A0A7S4L5L0_9EUKA8.200e-1132.12Hypothetical protein n=2 Tax=Paramoeba aestuarina ... [more]
A0A7S4V0Q8_9EUKA5.750e-1032.12Hypothetical protein n=1 Tax=Paramoeba aestuarina ... [more]
A0A7S4IHK8_9EUKA1.580e-831.85Hypothetical protein (Fragment) n=1 Tax=Vannella s... [more]
A0A7S4MHF2_9EUKA8.060e-830.60Hypothetical protein n=1 Tax=Vannella sp. CB-2014 ... [more]
A0A7S4JD38_9STRA1.110e-731.79Hypothetical protein n=1 Tax=Odontella aurita TaxI... [more]
A0A7S4JRH4_9EUKA1.170e-731.21Hypothetical protein n=1 Tax=Paramoeba aestuarina ... [more]
A0A367Q6V7_9NOSO1.450e-732.62Uncharacterized protein n=1 Tax=Nostoc sp. ATCC 43... [more]
A0A7S4UEQ9_9EUKA5.110e-736.73Hypothetical protein (Fragment) n=1 Tax=Paramoeba ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig95708contigM-pyrifera_M_contig95708:325..711 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Exons1
Model size387
Cds size387
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816871.9738827-CDS-M-pyrifera_M_contig95708:324..7111622816871.9738827-CDS-M-pyrifera_M_contig95708:324..711Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig95708 325..711 +
1692278228.043635-CDS-M-pyrifera_M_contig95708:324..7111692278228.043635-CDS-M-pyrifera_M_contig95708:324..711Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig95708 325..711 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig95708.22070.1prot_M-pyrifera_M_contig95708.22070.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig95708 325..711 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig95708.22070.1

>prot_M-pyrifera_M_contig95708.22070.1 ID=prot_M-pyrifera_M_contig95708.22070.1|Name=mRNA_M-pyrifera_M_contig95708.22070.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=129bp
PPVEELLCVYGKNLPTEVGYIYRPFKKTKFNTFGEPLTLKLDRSRSKAKR
VLEGTKGFLAKHGIIFEVPDRDEDIQSGDGTVPYASLAYHKKWENTLGAP
TITSVEIEGADDREMLSMPLVIGSLVRHI
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mRNA from alignment at M-pyrifera_M_contig95708:325..711+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig95708.22070.1 ID=mRNA_M-pyrifera_M_contig95708.22070.1|Name=mRNA_M-pyrifera_M_contig95708.22070.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=387bp|location=Sequence derived from alignment at M-pyrifera_M_contig95708:325..711+ (Macrocystis pyrifera P11B4 male)
CCCCCAGTCGAGGAGCTTCTTTGTGTGTATGGCAAGAACCTGCCCACGGA AGTTGGCTACATCTACCGCCCTTTCAAGAAGACTAAGTTCAACACTTTCG GCGAGCCGCTGACGCTGAAGCTGGACCGCTCTCGGAGCAAGGCCAAGCGG GTGCTCGAGGGCACGAAGGGATTCCTCGCTAAGCATGGCATCATCTTTGA AGTACCGGACAGGGACGAAGACATTCAGTCTGGTGACGGCACCGTCCCTT ACGCATCCCTCGCCTACCACAAGAAGTGGGAGAACACGCTTGGTGCGCCA ACGATCACATCTGTGGAAATTGAGGGCGCCGATGACAGGGAAATGCTGTC AATGCCTCTCGTCATTGGCTCCTTGGTTCGGCACATA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig95708:325..711+

>mRNA_M-pyrifera_M_contig95708.22070.1 ID=mRNA_M-pyrifera_M_contig95708.22070.1|Name=mRNA_M-pyrifera_M_contig95708.22070.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=774bp|location=Sequence derived from alignment at M-pyrifera_M_contig95708:325..711+ (Macrocystis pyrifera P11B4 male)
CCCCCAGTCGAGGAGCTTCTTTGTGTGTATGGCAAGAACCTGCCCACGGA
AGTTGGCTACATCTACCGCCCTTTCAAGAAGACTAAGTTCAACACTTTCG
GCGAGCCGCTGACGCTGAAGCTGGACCGCTCTCGGAGCAAGGCCAAGCGG
GTGCTCGAGGGCACGAAGGGATTCCTCGCTAAGCATGGCATCATCTTTGA
AGTACCGGACAGGGACGAAGACATTCAGTCTGGTGACGGCACCGTCCCTT
ACGCATCCCTCGCCTACCACAAGAAGTGGGAGAACACGCTTGGTGCGCCA
ACGATCACATCTGTGGAAATTGAGGGCGCCGATGACAGGGAAATGCTGTC
AATGCCTCTCGTCATTGGCTCCTTGGTTCGGCACATACCCCCAGTCGAGG
AGCTTCTTTGTGTGTATGGCAAGAACCTGCCCACGGAAGTTGGCTACATC
TACCGCCCTTTCAAGAAGACTAAGTTCAACACTTTCGGCGAGCCGCTGAC
GCTGAAGCTGGACCGCTCTCGGAGCAAGGCCAAGCGGGTGCTCGAGGGCA
CGAAGGGATTCCTCGCTAAGCATGGCATCATCTTTGAAGTACCGGACAGG
GACGAAGACATTCAGTCTGGTGACGGCACCGTCCCTTACGCATCCCTCGC
CTACCACAAGAAGTGGGAGAACACGCTTGGTGCGCCAACGATCACATCTG
TGGAAATTGAGGGCGCCGATGACAGGGAAATGCTGTCAATGCCTCTCGTC
ATTGGCTCCTTGGTTCGGCACATA
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