mRNA_M-pyrifera_M_contig9570.22066.1 (mRNA) Macrocystis pyrifera P11B4 male

BLAST of mRNA_M-pyrifera_M_contig9570.22066.1 vs. uniprot
Match: D7FTL9_ECTSI (Isoleucine--tRNA ligase n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FTL9_ECTSI)

HSP 1 Score: 928 bits (2398), Expect = 0.000e+0
Identity = 447/496 (90.12%), Postives = 471/496 (94.96%), Query Frame = 1

Query:    1 SPAFGEDDYRVCMAFGVIDKGEDIPCPVDSNGLFTSEVHNYAGQNVKSADKALCDEIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPHFVKEKRFQNWLEGAKDWAISRNRFWGTPMPMWVSEDGEEMVAIGSIAELKELSGVEVTDLHRESVDHITIPSKQGKGDLKRVEEVFDCWFESGSMPYAQIHYPFENKEKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGKKMSKRLKNYPDPMKVIDAHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTCAEQFVPNPEAAAASTNVMDAWIQAALQGLVKFVRTEMKAYRLYTVVPRLVDFIEQLTNWYVRLNRNRLKGSEGSDSARTGLFCMYEVLTTMSVLMAPITPFFSEYTYRHLRQCHPDRDNAS-VAEDAPGRATSVHMLMMPPVDESR 1485
            SPAFGEDDYRVC+A GVI KGE+IPCPVDSNGLFTSEV  YAGQ VK+ADKALCDEIKA GRLVSKDS  HSYPFCWRSDTPLIYKAVPSWFV VESIRDKLL +NA+ YWVP FV EKRFQNWLEGAKDWA+SRNRFWGTPMPMWVSEDGEEMVA+GSIAELKELSGVEVTDLHRESVDHITIPSKQGKGDLKRVEEVFDCWFESGSMPYAQ+HYPFENK+KFE+NFPADF+AEGLDQTRGWFYTLTVLGAALFDKPA+KNLIVNGLVLASDGKKMSKRLKNYPDPMKVID+HGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTC ++FVPNPEAAA STNVMD WIQA LQGLV+FV TEMKAYRLYTVVPRLV+FIEQLTNWYVRLNRNRLKG+EG++SA  GLFCMYEVL+TMSVLMAPITPFFSEYTYRHLR+CHPD+D    VAEDAPGRA+SVHMLMMP VDESR
Sbjct:  346 SPAFGEDDYRVCLAHGVIAKGEEIPCPVDSNGLFTSEVGEYAGQYVKTADKALCDEIKANGRLVSKDSCTHSYPFCWRSDTPLIYKAVPSWFVGVESIRDKLLKANAETYWVPAFVNEKRFQNWLEGAKDWAVSRNRFWGTPMPMWVSEDGEEMVAVGSIAELKELSGVEVTDLHRESVDHITIPSKQGKGDLKRVEEVFDCWFESGSMPYAQLHYPFENKDKFEKNFPADFIAEGLDQTRGWFYTLTVLGAALFDKPAFKNLIVNGLVLASDGKKMSKRLKNYPDPMKVIDSHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTCGDRFVPNPEAAAKSTNVMDNWIQATLQGLVQFVHTEMKAYRLYTVVPRLVEFIEQLTNWYVRLNRNRLKGAEGAESATAGLFCMYEVLSTMSVLMAPITPFFSEYTYRHLRECHPDKDGGKDVAEDAPGRASSVHMLMMPEVDESR 841          

BLAST of mRNA_M-pyrifera_M_contig9570.22066.1 vs. uniprot
Match: A0A7S3GN89_9STRA (Isoleucine--tRNA ligase (Fragment) n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3GN89_9STRA)

HSP 1 Score: 708 bits (1827), Expect = 5.380e-246
Identity = 339/497 (68.21%), Postives = 397/497 (79.88%), Query Frame = 1

Query:    1 SPAFGEDDYRVCMAFGVIDKGEDIPCPVDSNGLFTSEVHNYAGQNVKSADKALCDEIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPHFVKEKRFQNWLEGAKDWAISRNRFWGTPMPMWVSEDGEEMVAIGSIAELKELSGVEVTDLHRESVDHITIPSKQGKGDLKRVEEVFDCWFESGSMPYAQIHYPFENKEKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGKKMSKRLKNYPDPMKVIDAHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTCAEQFVPNPEAAAASTNVMDAWIQAALQGLVKFVRTEMKAYRLYTVVPRLVDFIEQLTNWYVRLNRNRLKGSEGSDSARTGLFCMYEVLTTMSVLMAPITPFFSEYTYRHLRQCHPDRDNA--SVAEDAPGRATSVHMLMMPPVDESR 1485
            +PAFGEDDYRVC+   ++ KGED+PCPVD+NGLFT+EV  + G+ VK AD  +C  +K  GRLV K+ Y HSYPFCWRS+TPLIYKAVPSWFV+VE I+ +LLA+N+  YWVP FV+EKRF NWL  AKDWAISRNRFWGTP+P+W+S+D EE+V IGS+ EL +LSGV VTDLH+E VDHITIPSKQGKG LKRV+EVFDCWFESGSMPYAQ+HYPFENKE+F   FPADF+AEGLDQTRGWFYTL V+   LFDKPA+KNLIVNGLVLA+DGKKMSKRLKNYP P  V++  GADALRLYLINSPVVRA+ LKF++EGV   VR VLLPWFN FRFF Q   R E     +FVPN E +  STN +D WI AA  GLV+FV  EMKAYRLYTVVPRLVDFI+QLT WY+RLNR R+KG    + A  GL  +YEVL TMS +MAP TPFF+EY Y+HLR+ HP+  +   +V  D+ GR+ SVH LM+P  DESR
Sbjct:  348 APAFGEDDYRVCLKNDIVAKGEDLPCPVDANGLFTAEVPEFVGRGVKEADNDICALLKNNGRLVLKEQYAHSYPFCWRSETPLIYKAVPSWFVSVEKIKPQLLANNSLTYWVPAFVQEKRFHNWLADAKDWAISRNRFWGTPIPLWISDDLEEIVTIGSVEELYQLSGVRVTDLHKEHVDHITIPSKQGKGLLKRVDEVFDCWFESGSMPYAQMHYPFENKERFTNGFPADFIAEGLDQTRGWFYTLMVISTCLFDKPAFKNLIVNGLVLAADGKKMSKRLKNYPAPEIVVNECGADALRLYLINSPVVRADVLKFQQEGVVEVVRGVLLPWFNTFRFFTQCVDRWESISGTKFVPNVEVSKGSTNDVDVWILAATVGLVEFVHQEMKAYRLYTVVPRLVDFIDQLTKWYIRLNRFRIKGESSQEEALVGLNVLYEVLATMSQIMAPFTPFFTEYLYQHLRKLHPNYGSTDPAVPVDSFGRSDSVHYLMLPVPDESR 844          

BLAST of mRNA_M-pyrifera_M_contig9570.22066.1 vs. uniprot
Match: A0A7S3XRA7_HETAK (Isoleucine--tRNA ligase n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XRA7_HETAK)

HSP 1 Score: 704 bits (1818), Expect = 5.950e-243
Identity = 331/493 (67.14%), Postives = 394/493 (79.92%), Query Frame = 1

Query:    1 SPAFGEDDYRVCMAFGVIDKGEDIPCPVDSNGLFTSEVHNYAGQNVKSADKALCDEIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPHFVKEKRFQNWLEGAKDWAISRNRFWGTPMPMWVSEDGEEMVAIGSIAELKELSGVEVTDLHRESVDHITIPSKQGKGDLKRVEEVFDCWFESGSMPYAQIHYPFENKEKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGKKMSKRLKNYPDPMKVIDAHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTCAE----QFVPNPEAAAASTNVMDAWIQAALQGLVKFVRTEMKAYRLYTVVPRLVDFIEQLTNWYVRLNRNRLKGSEGSDSARTGLFCMYEVLTTMSVLMAPITPFFSEYTYRHLRQCHPDRDNASVAEDAPGRATSVHMLMMP 1467
            +PAFGEDDYRVC+  G+I K E+ PCPVD NG FT  V ++ G+++K AD  +C  +K +GRLV K  Y HSYPFCWRSDTPLIYKAVPSWFV+VE I+++LLA+N Q YWVP FVKEKRF NWL  AKDWAISRNRFWGTP+P+W S+D +E+VA+GS+ EL+ LSGV V DLHRE +DHITIPSK+G+G L+RV+EVFDCWFESGSMPYAQ HYPFENKE+FE  FPADF+AEGLDQTRGWFYTL V+  ALFDKPA+KNLIVNGLVLA DGKKMSKRLKNYPDP  V+  +GADALRLYL+NSPVVRAE L+FKEEGV A V++V LPW+NAFRFF+Q ARR E+T  E    +F P+  AAA S N  DAWI AA + L+KFVR EM AYRLYTVVP L+ F+  LTNWY+RLNR+RLKG +G   A   L C+Y+VL  + +LMAP+TPFF+E  Y+HLR   P+  +A  AEDAPG+A SVH   +P
Sbjct:  266 APAFGEDDYRVCLEHGLIAKDEEAPCPVDFNGRFTEAVADFQGRHIKEADADICAHLKERGRLVQKAVYDHSYPFCWRSDTPLIYKAVPSWFVSVEKIKERLLANNDQTYWVPAFVKEKRFHNWLRDAKDWAISRNRFWGTPIPLWCSDDYQEIVAVGSVDELEALSGVRVEDLHREHIDHITIPSKEGRGALRRVDEVFDCWFESGSMPYAQQHYPFENKERFEAGFPADFIAEGLDQTRGWFYTLMVISTALFDKPAFKNLIVNGLVLAGDGKKMSKRLKNYPDPSIVVSKYGADALRLYLVNSPVVRAEPLRFKEEGVLAVVKDVFLPWYNAFRFFMQNARRWELTHPEGGGRRFAPDAAAAAGSANPTDAWILAAKEDLIKFVRQEMGAYRLYTVVPELLTFLNHLTNWYIRLNRDRLKGKDGPAEAHAALCCLYDVLFALCLLMAPLTPFFAETLYQHLRPFRPEAADAGAAEDAPGKAASVHYCTLP 758          

BLAST of mRNA_M-pyrifera_M_contig9570.22066.1 vs. uniprot
Match: W7U130_9STRA (Isoleucine--tRNA ligase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7U130_9STRA)

HSP 1 Score: 705 bits (1819), Expect = 1.030e-241
Identity = 339/507 (66.86%), Postives = 402/507 (79.29%), Query Frame = 1

Query:    1 SPAFGEDDYRVCMAFGVIDKGEDIPCPVDSNGLFTSEVHNYAGQNVKSADKALCDEIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPHFVKEKRFQNWLEGAKDWAISRNRFWGTPMPMWVSEDGEEMVAIGSIAELKELSGVE-VTDLHRESVDHITIPSKQGKG--DLKRVEEVFDCWFESGSMPYAQIHYPFENKEKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGKKMSKRLKNYPDPMKVIDAHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTCAEQFVPNPEAAAASTNVMDAWIQAALQGLVKFVRTEMKAYRLYTVVPRLVDFIEQLTNWYVRLNRNRLKGSEGSDSARTGLFCMYEVLTTMSVLMAPITPFFSEYTYRHLRQCHPDRDNASVAE---------DAPGRATSVHMLMMPPVDESR 1485
            +P FGEDDYRVC+A GVI KG ++PCPVDSNG FT +V ++ GQ+VK+AD  +C  +KAKGRLV K +Y HSYPFCWRSDTPLIYKAVPSWFVAV ++++KLLA+N + YWVP FVKE RF NWL  AKDWAISRNRFWGTP+P+W SED EE VAI SI +L+ LSGV+ +TDLHRESVD ITIPS +G     L+RV+EVFDCWFESGSMPYAQ+HYPF+NKE+FE NFPADF+AEGLDQTRGWFYTL V+   LFDKP ++NLIVNGLVLASDGKKMSKRLKNYP P  ++  +GADALRLYLI+SPVVRAE L+FKEE V   ++ V LPW NAFRFF Q   RLEM     F P+PE A ASTNVMD WIQA+LQG++KFV  EM AYRLYTV PRL+ FIE+LTNWYVRLNR RLKG EG + + TGL  +YEVL +M++LMAP TPF +E+ Y+HLR+ HP+  + S A          DA G+A SVH +M+P  DE+R
Sbjct:  348 APGFGEDDYRVCLAHGVIRKGTNVPCPVDSNGRFTPDVRDFVGQHVKAADGDICQLLKAKGRLVDKATYSHSYPFCWRSDTPLIYKAVPSWFVAVGTVKEKLLANNQKTYWVPAFVKEHRFHNWLADAKDWAISRNRFWGTPIPLWCSEDMEEQVAIESIEQLERLSGVKGITDLHRESVDQITIPSSRGPSYPPLRRVDEVFDCWFESGSMPYAQLHYPFDNKERFEANFPADFIAEGLDQTRGWFYTLMVISTCLFDKPPFQNLIVNGLVLASDGKKMSKRLKNYPPPDDILKKYGADALRLYLISSPVVRAEPLRFKEEDVLGMIKRVFLPWINAFRFFQQGVARLEMEKGSVFTPSPERARASTNVMDVWIQASLQGMIKFVHDEMAAYRLYTVTPRLLRFIEELTNWYVRLNRTRLKGQEGDEESLTGLSTLYEVLLSMTILMAPFTPFLTEFLYQHLRKIHPNFTSCSAAASSSVSALPLDAIGKADSVHYIMLPMYDEAR 854          

BLAST of mRNA_M-pyrifera_M_contig9570.22066.1 vs. uniprot
Match: A0A482S0Q8_9ARCH (Isoleucine--tRNA ligase n=1 Tax=archaeon TaxID=1906665 RepID=A0A482S0Q8_9ARCH)

HSP 1 Score: 701 bits (1809), Expect = 2.240e-241
Identity = 324/494 (65.59%), Postives = 402/494 (81.38%), Query Frame = 1

Query:    1 SPAFGEDDYRVCMAFGVIDKGEDIPCPVDSNGLFTSEVHNYAGQNVKSADKALCDEIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPHFVKEKRFQNWLEGAKDWAISRNRFWGTPMPMWVSEDGEEMVAIGSIAELKELSGVEVTDLHRESVDHITIPSKQGKGDLKRVEEVFDCWFESGSMPYAQIHYPFENKEKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGKKMSKRLKNYPDPMKVIDAHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTCAEQFVPNPEAAAASTNVMDAWIQAALQGLVKFVRTEMKAYRLYTVVPRLVDFIEQLTNWYVRLNRNRLKGSEGSDSARTGLFCMYEVLTTMSVLMAPITPFFSEYTYRHLRQCHPDRDNASVAEDAPGRATSVHMLMMPPVDES 1482
            +PAFGEDDYRVC+A  ++ KGE+I CPVDSNG FT+EV  ++G+ VK AD  +C  +K KGRLV K+ Y H YPFCWRS+TPLIYKAVPSWFV VE +R +LLA+NAQ YWVP+FV+EKRF NWL  AKDWAISRNRFWGTP+P+W+S+D EEMVAIGS+ EL+ LSG ++TDLH+E +D ITIPS++GKG L+R++EVFDCWFESGSMPYAQ+HYPFEN ++FE+ FPADF+AEGLDQTRGWFYTL V+   LFDKPA+KNLIVNGLVLA+DGKKMSKRLKNYPDP+ V++ +GADALRLYLINSPVVRA+ LKF+E+GV   +R V++PW+N+FRFF Q   R E +  + FVPN + A +S N +D WI AA  GLV+FV  EMKAYRLYTVVPRLV+FI++L+ WY+RLNR RLKG  G + A  GL  +YEVL TM+V+M+P TPFF+E+ Y+HLR+ HP+  N     D  G+A SVH +M+P  D+S
Sbjct:  278 APAFGEDDYRVCIANNIVGKGEEIVCPVDSNGRFTNEVPEFSGRAVKEADNDICALLKNKGRLVLKEVYAHQYPFCWRSETPLIYKAVPSWFVTVEKLRPQLLANNAQTYWVPNFVQEKRFHNWLADAKDWAISRNRFWGTPIPLWISDDLEEMVAIGSVEELERLSGAKITDLHKEFIDPITIPSQKGKGVLRRIDEVFDCWFESGSMPYAQLHYPFENADRFEKGFPADFIAEGLDQTRGWFYTLMVISTCLFDKPAFKNLIVNGLVLAADGKKMSKRLKNYPDPVLVVNNYGADALRLYLINSPVVRADVLKFQEDGVSEVLRGVMIPWYNSFRFFTQCVDRWEGSTGKTFVPNAQLARSSQNDVDIWILAASLGLVEFVHQEMKAYRLYTVVPRLVEFIDELSKWYLRLNRFRLKGEMGDEDAAVGLNVLYEVLVTMTVIMSPFTPFFTEFLYQHLRKLHPNYTNDQAGVDVFGKAASVHFIMLPEADKS 771          

BLAST of mRNA_M-pyrifera_M_contig9570.22066.1 vs. uniprot
Match: A0A7S2WSX5_9STRA (Isoleucine--tRNA ligase n=2 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2WSX5_9STRA)

HSP 1 Score: 686 bits (1769), Expect = 1.470e-234
Identity = 319/492 (64.84%), Postives = 397/492 (80.69%), Query Frame = 1

Query:    1 SPAFGEDDYRVCMAFGVIDKGEDIPCPVDSNGLFTSEVHNYAGQNVKSADKALCDEIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPHFVKEKRFQNWLEGAKDWAISRNRFWGTPMPMWVSEDGEEMVAIGSIAELKELSGV---EVTDLHRESVDHITIPSKQGKGDLKRVEEVFDCWFESGSMPYAQIHYPFENKEKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGKKMSKRLKNYPDPMKVIDAHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTCAEQFVPNPEAAAASTNVMDAWIQAALQGLVKFVRTEMKAYRLYTVVPRLVDFIEQLTNWYVRLNRNRLKGSEGSDSARTGLFCMYEVLTTMSVLMAPITPFFSEYTYRHLRQCHPDRDNASVAEDAPGRATSVHMLMMP 1467
            +PAFGEDDYRVC+  GVI KG  +PCPVD NG +T+EV ++ GQ+VK AD A+CD +KA+GRLV+   Y+HSYPFCWRSDTPLIYKAVPSWFVAVE  +D+LL +N Q YWVP+FVKE RF NWL+ A+DWAISRNRFWGTP+P+W SED +E++AIGS+AEL+EL+G+    VTDLHRES+D I IPSKQGKG+L+RV+EVFDCWFESGSMPYAQ+HYPFENKE+FE+ FPA F+AEGLDQTRGWFYTL VL  ALFD+PA+ NLIVNG+VLA+DGKKMSKRLKNYPDP  VI  +GADALR+YLINSPVVRAE LKF E GV+   R+V+LPW+N FRFF+Q A R ++   + F P+   A++S N ++ WI+A LQ L+ FV  EM  YRLYTV+PRLV F+EQL+ WY+RLNR+RL+G++G + A  GL  +Y V+ +++ +M+P TPFFSE+ Y+ LRQ HPD   A   ++A G A SVH L +P
Sbjct:  346 APAFGEDDYRVCLKHGVIAKGVQLPCPVDFNGCYTAEVSDFEGQHVKQADAAICDMLKAQGRLVASAKYLHSYPFCWRSDTPLIYKAVPSWFVAVEQFKDRLLKNNEQTYWVPNFVKEGRFHNWLKEARDWAISRNRFWGTPIPIWASEDMQELIAIGSVAELEELAGLPKGSVTDLHRESIDDIVIPSKQGKGNLRRVDEVFDCWFESGSMPYAQLHYPFENKERFEKGFPAHFIAEGLDQTRGWFYTLMVLSTALFDRPAFSNLIVNGMVLAADGKKMSKRLKNYPDPNAVIATYGADALRMYLINSPVVRAEPLKFTEPGVKGVARDVILPWYNTFRFFLQNAHRWQLATGKTFRPDAALASSSANPINVWIRAELQSLIAFVHEEMSKYRLYTVMPRLVSFLEQLSKWYLRLNRDRLRGADGEEEALVGLSVLYFVVISVASIMSPFTPFFSEFLYQKLRQLHPDF--AKPDKEAMGSAQSVHFLTLP 835          

BLAST of mRNA_M-pyrifera_M_contig9570.22066.1 vs. uniprot
Match: A0A8K1FDR5_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FDR5_PYTOL)

HSP 1 Score: 684 bits (1766), Expect = 3.920e-234
Identity = 332/497 (66.80%), Postives = 393/497 (79.07%), Query Frame = 1

Query:    1 SPAFGEDDYRVCMAFGVIDKGEDIPCPVDSNGLFTSEVHNYAGQNVKSADKALCDEIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPHFVKEKRFQNWLEGAKDWAISRNRFWGTPMPMWVSEDGEEMVAIGSIAELKELSGVEVTDLHRESVDHITIPSKQGKGDLKRVEEVFDCWFESGSMPYAQIHYPFENKEKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGKKMSKRLKNYPDPMKVIDAHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTCAEQFVPNPEAAAASTNVMDAWIQAALQGLVKFVRTEMKAYRLYTVVPRLVDFIEQLTNWYVRLNRNRLKGSEGSDSARTGLFCMYEVLTTMSVLMAPITPFFSEYTYRHLRQCHPDRDN--ASVAEDAPGRATSVHMLMMPPVDESR 1485
            +P FGEDDYRVC+  GV DK  D+P  +D NG FT  V    G +VK AD  +C +IK +GRLVSK++ VHSYPFC+RS TPLIY+AVP WFV VE IR++++ +N Q YWVP FVKEKRF NWL   KDW ISR RFWGTP+P+WVSED EE+V IGSI EL+ELSG +V DLHRE +DHITIPSKQGKG L+RV+EVFDCWFESGSMPYAQ HYPFENKEKF+  FPADF+AEGLDQTRGWFYTL V+  ALFDKPA+KN+IVNGLVLA DG+KMSK L+NY DP ++ + +GADALRLYLINSP VRAE +KF + GV + +R++ LPW+N+ RFF Q A RLE+   + FVP+ E A +S NVMD+WI AAL  ++KFV TEMKAYRLYTVVPRLV+FI+QLT WYVRLNR RLKGSEG+D AR  L  +YEVL T+S LMAP TPFF+EY Y+ LRQ HP+  N  A +AEDA G A SVH LM+P  D SR
Sbjct:  335 APTFGEDDYRVCVKHGVCDKF-DLPNSLDDNGRFTDAVPFVKGLHVKEADDIICKDIKERGRLVSKNTIVHSYPFCYRSGTPLIYRAVPGWFVNVEKIRERIVENNRQTYWVPSFVKEKRFHNWLVDGKDWNISRGRFWGTPLPLWVSEDFEEVVCIGSIKELEELSGEKVEDLHREFIDHITIPSKQGKGTLRRVDEVFDCWFESGSMPYAQQHYPFENKEKFDAGFPADFIAEGLDQTRGWFYTLMVISTALFDKPAFKNVIVNGLVLAEDGRKMSKSLRNYTDPNEIFERYGADALRLYLINSPGVRAEPIKFVDSGVLSVIRDLFLPWYNSARFFSQSATRLELATGKTFVPSSEEALSSKNVMDSWIIAALHNVIKFVHTEMKAYRLYTVVPRLVEFIDQLTKWYVRLNRPRLKGSEGNDEARIALSSLYEVLYTISKLMAPFTPFFAEYMYQFLRQFHPNVVNNVAGLAEDADGVAKSVHFLMVPDYDASR 830          

BLAST of mRNA_M-pyrifera_M_contig9570.22066.1 vs. uniprot
Match: A0A2D4BRB4_PYTIN (Isoleucine--tRNA ligase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BRB4_PYTIN)

HSP 1 Score: 684 bits (1765), Expect = 5.390e-234
Identity = 332/497 (66.80%), Postives = 389/497 (78.27%), Query Frame = 1

Query:    1 SPAFGEDDYRVCMAFGVIDKGEDIPCPVDSNGLFTSEVHNYAGQNVKSADKALCDEIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPHFVKEKRFQNWLEGAKDWAISRNRFWGTPMPMWVSEDGEEMVAIGSIAELKELSGVEVTDLHRESVDHITIPSKQGKGDLKRVEEVFDCWFESGSMPYAQIHYPFENKEKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGKKMSKRLKNYPDPMKVIDAHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTCAEQFVPNPEAAAASTNVMDAWIQAALQGLVKFVRTEMKAYRLYTVVPRLVDFIEQLTNWYVRLNRNRLKGSEGSDSARTGLFCMYEVLTTMSVLMAPITPFFSEYTYRHLRQCHPDRDN--ASVAEDAPGRATSVHMLMMPPVDESR 1485
            +P FGEDDYRVC+  GV+DK E +P P+D NG FT  V    G +VK AD  +C+ +K K RL+ K   VHSYPFC+RS TPLIY+AVP WFV VE IRD+++ +N Q YWVP FVKEKRF NWL   KDW ISR RFWGTP+P+WVSED EE+V +GSI EL+ELSG +VTDLHRE +DHITIPS+QGKG L+RVEEVFDCWFESGSMPYAQ HYPFENKE FE ++PADF+AEGLDQTRGWFYTL V+  ALFDKPA+KNLIVNGLVLA DG+KMSK L+N+ DP ++++ +GADALRLYLINSPVVRAE LKF + GV   +RE+ LPW+N+ RFF Q A RLE T  + FVP+   A AS NVMD+WI AAL  L+KFV TEM AYRLYTVVPRLV+FI+QLT WYVRLNR RLKGSEG+D A   L  +YEVL T+  LMAP TPFF+EY Y+ LRQ HP+  N  A +AEDA G A SVH LM+P  D SR
Sbjct:  334 APTFGEDDYRVCVKQGVVDKFE-LPNPLDDNGRFTDAVPFVKGLHVKEADPIVCNLLKEKDRLIRKGQIVHSYPFCYRSGTPLIYRAVPGWFVNVEKIRDRIVENNRQTYWVPSFVKEKRFHNWLVDGKDWNISRGRFWGTPLPLWVSEDFEEIVCVGSIQELEELSGEKVTDLHREFIDHITIPSRQGKGQLRRVEEVFDCWFESGSMPYAQKHYPFENKEAFEASYPADFIAEGLDQTRGWFYTLMVISTALFDKPAFKNLIVNGLVLAEDGRKMSKSLRNFTDPTEILERYGADALRLYLINSPVVRAEPLKFVDSGVMGVIRELFLPWYNSVRFFSQNATRLEETTGQAFVPSSAEALASKNVMDSWIIAALHNLIKFVHTEMAAYRLYTVVPRLVEFIDQLTKWYVRLNRPRLKGSEGNDDAHVALSAVYEVLYTLCKLMAPFTPFFAEYMYQFLRQYHPNVVNQVAGLAEDADGVARSVHFLMVPDFDASR 829          

BLAST of mRNA_M-pyrifera_M_contig9570.22066.1 vs. uniprot
Match: W2Q337_PHYPN (Isoleucine--tRNA ligase n=29 Tax=Peronosporaceae TaxID=4777 RepID=W2Q337_PHYPN)

HSP 1 Score: 682 bits (1761), Expect = 2.480e-233
Identity = 333/498 (66.87%), Postives = 390/498 (78.31%), Query Frame = 1

Query:    1 SPAFGEDDYRVCMAFGVIDKGEDIPCPVDSNGLFTSEVHNYAGQNVKSADKALCDEIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPHFVKEKRFQNWLEGAKDWAISRNRFWGTPMPMWVSEDGEEMVAIGSIAELKELSGVEVTDLHRESVDHITIPSKQGKGDLKRVEEVFDCWFESGSMPYAQIHYPFENKEKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGKKMSKRLKNYPDPMKVIDAHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTCAEQFVPNPEAAAASTNVMDAWIQAALQGLVKFVRTEMKAYRLYTVVPRLVDFIEQLTNWYVRLNRNRLKGSEGSDSARTGLFCMYEVLTTMSVLMAPITPFFSEYTYRHLRQCHPDRDNAS---VAEDAPGRATSVHMLMMPPVDESR 1485
            +P FGEDDYRVC+  GV DK   +P P+D NG+FT  V    G +VK AD  +C ++K +GRLVSK + VHSYPFC+RS TPLIY+A+P WFV VE IRD+++A+N   YWVP FV+EKRF NWL   KDW ISR RFWGTP+P+WVS+D EE+V +GSIAEL+EL+G +VTDLHRE +DH+TIPSKQGKG L+RV EVFDCWFESGSMPYAQ HYPFENK+KFE NFPADFVAEGLDQTRGWFYTL VL  ALFDKPA+KNLIVNGLVLA DG+KMSK LKN+ DP +++  +GADALRLYLINSPVVRAE LKF+  GV   +RE+ LPWFN+ RFF QQA RL++     FVPN EAA ASTNVMD+WI AA+  L+KFV  EMKAYRLYTVVPRLV FI QLTNWYVRLNR RLKGS GS  A   L  +YEV   ++ LMAP TPFF+EY Y+ LRQ HP+  N +   +AEDA G + SVH LM+P  D SR
Sbjct:  336 APTFGEDDYRVCVREGVADKFT-LPDPLDDNGVFTDAVPLVKGLHVKKADDVICQDLKNRGRLVSKGTEVHSYPFCYRSGTPLIYRAIPGWFVNVERIRDRIVANNKLTYWVPSFVQEKRFHNWLVDGKDWNISRGRFWGTPLPLWVSDDYEEVVCVGSIAELEELAGEKVTDLHREFIDHLTIPSKQGKGVLRRVPEVFDCWFESGSMPYAQQHYPFENKDKFEANFPADFVAEGLDQTRGWFYTLMVLSTALFDKPAFKNLIVNGLVLAEDGRKMSKSLKNFTDPEEILQKYGADALRLYLINSPVVRAEPLKFQAPGVLGVIREIFLPWFNSARFFAQQATRLQLETGVAFVPNREAALASTNVMDSWIIAAMHNLIKFVHEEMKAYRLYTVVPRLVSFIGQLTNWYVRLNRPRLKGSAGSAEAAVALSALYEVEYNLAKLMAPFTPFFTEYMYQFLRQFHPNVVNGAGKDLAEDADGVSPSVHFLMLPDFDASR 832          

BLAST of mRNA_M-pyrifera_M_contig9570.22066.1 vs. uniprot
Match: B8C8U0_THAPS (Isoleucine--tRNA ligase (Fragment) n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8C8U0_THAPS)

HSP 1 Score: 676 bits (1743), Expect = 3.920e-232
Identity = 330/502 (65.74%), Postives = 389/502 (77.49%), Query Frame = 1

Query:    1 SPAFGEDDYRVCMAFGVIDKGEDIPCPVDSNGLFTSEVHNYAGQNVKSADKALCDEIKAKGRLVSKDSYVHSYPFCWRSDTPLIYKAVPSWFVAVESIRDKLLASNAQAYWVPHFVKEKRFQNWLEGAKDWAISRNRFWGTPMPMWVSEDGEEMVAIGSIAELKELSGVEVTDLHRESVDHITIPSKQGKG-DLKRVEEVFDCWFESGSMPYAQIHYPFENKEKFERNFPADFVAEGLDQTRGWFYTLTVLGAALFDKPAYKNLIVNGLVLASDGKKMSKRLKNYPDPMKVIDAHGADALRLYLINSPVVRAESLKFKEEGVQATVREVLLPWFNAFRFFVQQARRLEMTCAEQFVPNPEAAAASTNVMDAWIQAALQGLVKFVRTEMKAYRLYTVVPRLVDFIEQLTNWYVRLNRNRLKGSEGSDS-ARTGLFCMYEVLTTMSVLMAPITPFFSEYTYRHLRQCHPDRDNASVAEDA-----PGRATSVHMLMMPPVDESR 1485
            +PAFGEDDYRVC+A  VI+KG D+PCPVDSNG+FT +V    G +VK AD  L   IK KGRLV KD+  HSYPFCWRSDTPLIYKAVPSWF+ VE IRD+++ +N + YWVP+ VKE RF +WL  A+DWA+SRNRFWGTP+P+W +ED  E+V IGS+ EL ELSGV V DLH++ VD ITIPSK+  G +L RV+EVFDCWFESGSMPYAQ+HYPFENKEKFE  FPADF+AEGLDQTRGWFYTL VL  ALFDKPA+KNLIVNGLVLASDGKKMSKRLKNYPDP  VI  +GADALR+YLINSPVVRAESLKF+E GV   V+EV LP +NAFRFFVQ   R E     +FVP+ E   ++ N  D WI AA QGL+KFV  EM AYRLYTV+P LV F+ QLTNWYVRLNR+RLKG EG +S + TGL  +Y+VL  +++LMAP TPF +EY Y+HLR+  P   +A+    A     PG++ SVH L +P  DESR
Sbjct:  339 APAFGEDDYRVCLAHKVIEKGNDLPCPVDSNGMFTEQVPEVKGLHVKKADDNLIKLIKDKGRLVQKDNLDHSYPFCWRSDTPLIYKAVPSWFIKVEEIRDRIVENNKKTYWVPNQVKEGRFHSWLTDARDWAVSRNRFWGTPIPIWCNEDMTEVVCIGSVDELAELSGVRVDDLHKDVVDKITIPSKKNPGTELHRVDEVFDCWFESGSMPYAQMHYPFENKEKFENGFPADFIAEGLDQTRGWFYTLMVLSTALFDKPAFKNLIVNGLVLASDGKKMSKRLKNYPDPNVVISKYGADALRMYLINSPVVRAESLKFQESGVLGVVKEVFLPLYNAFRFFVQNTERWEAKNGSKFVPSKEKVQSTANPTDVWISAATQGLIKFVHEEMAAYRLYTVMPALVSFVTQLTNWYVRLNRDRLKGLEGGESESETGLQVLYDVLLDVTILMAPFTPFITEYFYQHLRKMQPSYADAANGGGATNPTMPGKSDSVHFLNLPAYDESR 840