mRNA_M-pyrifera_M_contig95615.22045.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig95615.22045.1
Unique NamemRNA_M-pyrifera_M_contig95615.22045.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig95615.22045.1 vs. uniprot
Match: A0A5A8DQ05_CAFRO (Uncharacterized protein n=3 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DQ05_CAFRO)

HSP 1 Score: 268 bits (686), Expect = 1.070e-81
Identity = 125/177 (70.62%), Postives = 140/177 (79.10%), Query Frame = 1
Query:    4 TTAVLGKPRDFPQFGFDNEYGKRVVCVPSFAASRFLVSNAEFLPFVLAGGYRERKYWVSELGDDEGWRWVQYRNATHPSFWVATKHMPEYFGGTDDLPYQKDDGSDLAGSGEEFKLRTEFHIVPMPWDWPVEVNVHESKAFLRWKAEQEGTGV-RYRLPTEAEHKVMRADPSPHPES 531
            T  VLGKP DFPQ+G+DNEYGKRVV VP+FA SRF V+NAEFLPFVLAGGYR+RK+WVS  GDDEGWRW  YRNATHPSFWVAT  MP++ GGT +LPYQKDDG   AG+G EF LR E  IVPMPWDWPVEVN HE++AFL WKA  EG    + R+PTEAE   MR DP P PE+
Sbjct:  255 TRVVLGKPCDFPQYGWDNEYGKRVVDVPAFAGSRFKVTNAEFLPFVLAGGYRDRKWWVSPRGDDEGWRWASYRNATHPSFWVATAAMPDFIGGTPELPYQKDDGHARAGTGTEFMLRVELDIVPMPWDWPVEVNYHEARAFLNWKAANEGLAPGQLRMPTEAEFHAMRDDPCPFPEA 431          
BLAST of mRNA_M-pyrifera_M_contig95615.22045.1 vs. uniprot
Match: A0A813CMR4_9DINO (Egt-1 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813CMR4_9DINO)

HSP 1 Score: 252 bits (643), Expect = 1.970e-75
Identity = 114/174 (65.52%), Postives = 139/174 (79.89%), Query Frame = 1
Query:   10 AVLGKPRDFPQFGFDNEYGKRVVCVPSFAASRFLVSNAEFLPFVLAGGYRERKYWVSELGDDEGWRWVQYRNATHPSFWVATKHMPEYFGGTDDLPYQKDDGSDLAGSGEEFKLRTEFHIVPMPWDWPVEVNVHESKAFLRWKAEQEGTGVRYRLPTEAEHKVMRADPSPHPES 531
             VLGKP+DFP +G+DNEYG R V VP+F A+ FLV+NAEFLPFV+AGGY ++KYWVS  GDDEGWRWV +RNATHPS+WVATK MP++ GG   LPYQKDDG++ AG G +F+LR  F IV MPWDWPVEVN  E++AFL WK E+   GV++R+PTEAE+ V+RADP   P S
Sbjct:  275 VVLGKPQDFPSYGWDNEYGSRKVAVPAFKAASFLVTNAEFLPFVMAGGYTQKKYWVSPAGDDEGWRWVTFRNATHPSWWVATKAMPQFIGGKPGLPYQKDDGTEAAGDGAQFRLRVMFDIVDMPWDWPVEVNYLEARAFLNWK-EECACGVKFRMPTEAEYHVLRADPCALPGS 447          
BLAST of mRNA_M-pyrifera_M_contig95615.22045.1 vs. uniprot
Match: A0A812WHX8_9DINO (Hypothetical protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A812WHX8_9DINO)

HSP 1 Score: 229 bits (584), Expect = 1.600e-70
Identity = 116/221 (52.49%), Postives = 134/221 (60.63%), Query Frame = 1
Query:   10 AVLGKPRDFPQFGFDNEYGKRVVCVPSFAASRFLVSNAEFLPFVLAGGYRERKYWVSELGDDEGWRWVQYRNATHPSFWVATKHMPEYFGGTDDLPYQKDDGSDLAGSGEEFKLRTEF-------------------------------HIVPMPWDWPVEVNVHESKAFLRWK-------------------AEQEGTGVRYRLPTEAEHKVMRADPSPH 522
            A LGKPRDFP FG+DNEYG+R V VP FAAS++LVSN EF PFV+A GY  ++YW++E GDDEGWRWV YRNA HPSFWVAT  MPEYFGG  + PYQKDDG   AG G++FK R  F                                I+ MPWDWPVEVN  E+KAFLRWK                   A+ E     YRLPTEAE+ + RADPSP+
Sbjct:   89 ATLGKPRDFPSFGWDNEYGRRDVDVPEFAASKYLVSNGEFYPFVMANGYETKRYWIAEDGDDEGWRWVTYRNAKHPSFWVATADMPEYFGGRPEYPYQKDDGHPKAGKGKQFKYRAMFDVRMITPLAFFDSGGHAVFLKFDLFLLLVRSQIIDMPWDWPVEVNCLEAKAFLRWKVSCHKEQSFTAKIVYVQWVAQGEQDKAVYRLPTEAEYFLCRADPSPY 309          
BLAST of mRNA_M-pyrifera_M_contig95615.22045.1 vs. uniprot
Match: A0A7S1C2I6_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1C2I6_9STRA)

HSP 1 Score: 204 bits (519), Expect = 2.340e-59
Identity = 96/180 (53.33%), Postives = 127/180 (70.56%), Query Frame = 1
Query:   10 AVLGKPRDFPQFGFDNEYGKRVVCVPSFAASRFLVSNAEFLPFVLAGGYRERKYWVSELGDDEGWRWVQYRNATHPSFWVATKH------MPEYFGGTDDLPYQKDDGSDLAGSGEEFKLRTEFHIVPMPWDWPVEVNVHESKAFLRWKAEQEGTGVRYRLPTEAEHKVMRADPSPHPES 531
            A +GKP DFP +G+DNEYG+R V VP+F  S+FLV+NAEFLPFVL GGY ++++W+S+ GDDEGWRW+Q R    P +WVAT H      +    G +     + DDG+  AG G  F+LR  F I+ MPWDWPVEVN  E+ AFL WK+ +EG  V+YR+PTEAE+ +MRA P+P+ E+
Sbjct:  265 ATIGKPLDFPTYGWDNEYGRRDVDVPAFKGSKFLVTNAEFLPFVLDGGYADKRWWISKSGDDEGWRWIQLRGQKKPLWWVATSHPDLAPFVGNRPGSSPTHQCELDDGTAAAGDGPSFRLRAMFDIIDMPWDWPVEVNYLEAVAFLNWKSAREG--VQYRVPTEAEYHLMRASPNPYDEA 442          
BLAST of mRNA_M-pyrifera_M_contig95615.22045.1 vs. uniprot
Match: UPI00096B3A90 (ergothioneine biosynthesis protein 1-like isoform X1 n=4 Tax=Amphimedon queenslandica TaxID=400682 RepID=UPI00096B3A90)

HSP 1 Score: 167 bits (423), Expect = 4.890e-45
Identity = 77/173 (44.51%), Postives = 106/173 (61.27%), Query Frame = 1
Query:    1 ETTAVLGKPRDFPQFGFDNEYGKRVVCVPSFAASRFLVSNAEFLPFVLAGGYRERKYWVSELGDDEGWRWVQYRNATHPSFWVATKHMPEYFGGT-DDLPYQKDDGSDLAGSG--EEFKLRTEFHIVPMPWDWPVEVNVHESKAFLRWKAEQEGTGVRYRLPTEAEHKVMRAD 510
            E    LGKP DFP +G+DNEYG + + VP+F A+++L++N EFL FV  GGY  +KYW      DEGW+WVQ+R   HP FWV T       G    D  +  ++ + +       ++K R  + ++ MPWDWPV+VN HE+KA+ RWK      G  +RLPTEAEH++MR D
Sbjct:  223 EGEITLGKPTDFPTYGWDNEYGTKTIKVPAFEATQYLITNKEFLDFVADGGYSTKKYWT-----DEGWQWVQFRQPRHPLFWVCTDECKSNCGSILSDYSHCNNESNGVTNGSTHSKYKYRALYDVIDMPWDWPVDVNYHEAKAYCRWK------GAEFRLPTEAEHQIMRGD 384          
BLAST of mRNA_M-pyrifera_M_contig95615.22045.1 vs. uniprot
Match: A0A496UZX4_9GAMM (FGE-sulfatase domain-containing protein n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A496UZX4_9GAMM)

HSP 1 Score: 159 bits (401), Expect = 5.260e-45
Identity = 79/164 (48.17%), Postives = 105/164 (64.02%), Query Frame = 1
Query:   13 VLGKPRDFPQFGFDNEYGKRVVCVPSFAASRFLVSNAEFLPFVLAGGYRERKYWVSELGDDEGWRWVQYRNATHPSFWVATKHMPEYFGGTDDLPYQKDDGSDLAGSGEEFKLRTEFHIVPMPWDWPVEVNVHESKAFLRWKAEQEGTGVRYRLPTEAEHKVMR 504
             LGKP+D+P FG+DNEYG+R + V  F AS++LVSN EF  FV AGGYRE++YW +E     GW+W ++RN   P+FWVA  + P                   AG  +++KLRT F I+ +PW WP +VN HE+KA+  W+ EQEGT + YR+ TEAEH  MR
Sbjct:   24 TLGKPQDWPTFGWDNEYGQRSLRVRPFRASQYLVSNGEFYQFVKAGGYREQRYWTAE-----GWQWRKFRNVKWPTFWVA--YGP-------------------AGQ-QQYKLRTCFEIIDIPWSWPADVNYHEAKAYCAWRTEQEGTEMPYRVMTEAEHNRMR 160          
BLAST of mRNA_M-pyrifera_M_contig95615.22045.1 vs. uniprot
Match: A0A1X7UFB6_AMPQE (Uncharacterized protein n=2 Tax=Amphimedon queenslandica TaxID=400682 RepID=A0A1X7UFB6_AMPQE)

HSP 1 Score: 167 bits (423), Expect = 1.100e-44
Identity = 77/173 (44.51%), Postives = 106/173 (61.27%), Query Frame = 1
Query:    1 ETTAVLGKPRDFPQFGFDNEYGKRVVCVPSFAASRFLVSNAEFLPFVLAGGYRERKYWVSELGDDEGWRWVQYRNATHPSFWVATKHMPEYFGGT-DDLPYQKDDGSDLAGSG--EEFKLRTEFHIVPMPWDWPVEVNVHESKAFLRWKAEQEGTGVRYRLPTEAEHKVMRAD 510
            E    LGKP DFP +G+DNEYG + + VP+F A+++L++N EFL FV  GGY  +KYW      DEGW+WVQ+R   HP FWV T       G    D  +  ++ + +       ++K R  + ++ MPWDWPV+VN HE+KA+ RWK      G  +RLPTEAEH++MR D
Sbjct:  793 EGEITLGKPTDFPTYGWDNEYGTKTIKVPAFEATQYLITNKEFLDFVADGGYSTKKYWT-----DEGWQWVQFRQPRHPLFWVCTDECKSNCGSILSDYSHCNNESNGVTNGSTHSKYKYRALYDVIDMPWDWPVDVNYHEAKAYCRWK------GAEFRLPTEAEHQIMRGD 954          
BLAST of mRNA_M-pyrifera_M_contig95615.22045.1 vs. uniprot
Match: UPI001C3F7935 (uncharacterized protein LOC121848036 n=1 Tax=Callorhinchus milii TaxID=7868 RepID=UPI001C3F7935)

HSP 1 Score: 162 bits (409), Expect = 2.440e-43
Identity = 83/191 (43.46%), Postives = 102/191 (53.40%), Query Frame = 1
Query:   10 AVLGKPRDFPQFGFDNEYGKRVVCVPSFAASRFLVSNAEFLPFVLAGGYRERKYWVSELGDDEGWRWVQYRNATHPSFWVATKHMPEYFG---------------------GTDDLPYQKDDGSDLAGSGEEFKLRTEFHIVPMPWDWPVEVNVHESKAFLRWKAEQEGTGVRYRLPTEAEHKVMRADPSP 519
               GK RDFP +G+DNEYG+  V  P F A+R+L +NAEFL FVLAGGY+   YW      +EGW W Q+R A HP+FWV  K    + G                     GT      + +GS       EF  R  F ++PMPWDWPVEVN HE+KAF  W+      G  YRLP+EAEH+ MR    P
Sbjct:   25 VTYGKARDFPSYGWDNEYGEMTVETPEFEAARYLTTNAEFLRFVLAGGYQTHSYW-----SEEGWAWRQFRQAKHPTFWVCAKGCKAHCGSGLTDYSHCQLLIAPITNGGTGTPSSRGSQQEGSLHQDKASEFSYRAMFDVLPMPWDWPVEVNYHEAKAFCAWR------GPEYRLPSEAEHQRMRGTQKP 204          
BLAST of mRNA_M-pyrifera_M_contig95615.22045.1 vs. uniprot
Match: A0A1S3K4W5_LINUN (uncharacterized protein LOC106178593 isoform X1 n=3 Tax=Lingula unguis TaxID=7574 RepID=A0A1S3K4W5_LINUN)

HSP 1 Score: 160 bits (406), Expect = 1.180e-42
Identity = 73/170 (42.94%), Postives = 106/170 (62.35%), Query Frame = 1
Query:    1 ETTAVLGKPRDFPQFGFDNEYGKRVVCVPSFAASRFLVSNAEFLPFVLAGGYRERKYWVSELGDDEGWRWVQYRNATHPSFWVATKHMPEYFGGTDDLPYQKDDGSDLAGSGEEFKLRTEFHIVPMPWDWPVEVNVHESKAFLRWKAEQEGTGVRYRLPTEAEHKVMRAD 510
            +TT  LGKP+DFP +G+DNEYG+    VP F A++++++N E++ FV +GGY++ ++W  E     GW W QYR A HP +WV ++      G             D+ G+ E+F+LR  + ++ MPWDWP EVN HE+KA+ +WK      G  YRLPTEAE+ VMR +
Sbjct:  213 QTTVTLGKPQDFPSYGWDNEYGELECKVPPFRANKYIITNREYMEFVESGGYQDSQFWSKE-----GWSWKQYRQANHPLYWVCSQGCKNGCGSNLSASSHCRP-QDMEGTNEKFRLRAVYDVIDMPWDWPAEVNYHEAKAYAKWK------GPSYRLPTEAEYHVMRGE 370          
BLAST of mRNA_M-pyrifera_M_contig95615.22045.1 vs. uniprot
Match: UPI001B88E2C4 (LOW QUALITY PROTEIN: ergothioneine biosynthesis protein 1-like n=1 Tax=Gigantopelta aegis TaxID=1735272 RepID=UPI001B88E2C4)

HSP 1 Score: 158 bits (400), Expect = 8.130e-42
Identity = 77/179 (43.02%), Postives = 105/179 (58.66%), Query Frame = 1
Query:    1 ETTAVLGKPRDFPQFGFDNEYGKRVVCVPSFAASRFLVSNAEFLPFVLAGGYRERKYWVSELGDDEGWRWVQYRNATHPSFWVATKHMPEYFGGTD------DLPYQKDDGSDLAGSGEEFKLRTEFHIVPMPWDWPVEVNVHESKAFLRWKAEQEGTGVRYRLPTEAEHKVMRADPSP 519
            E    LGKP DFP +G+DNEYG R + VP+F A+++L +N EF  FV AGGY+ R+ W      DEGW+WVQYR+A HP FWV  +      G         +LPY  +  +D       ++ R  + ++ MPW+WPV+VN HE+KA+ +WK      G  +RLPTEAEH  M  +  P
Sbjct:  237 EGDVTLGKPNDFPTYGWDNEYGTRTMKVPAFEATKYLTTNREFREFVAAGGYQRRELWT-----DEGWQWVQYRHAKHPIFWVCHEGCKSNCGSDXSSYSHCNLPYTNETSADY------YRYRAMYDVIDMPWEWPVDVNYHEAKAYCQWK------GPEFRLPTEAEHHRMAGEQPP 398          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig95615.22045.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8DQ05_CAFRO1.070e-8170.62Uncharacterized protein n=3 Tax=Cafeteria roenberg... [more]
A0A813CMR4_9DINO1.970e-7565.52Egt-1 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=2... [more]
A0A812WHX8_9DINO1.600e-7052.49Hypothetical protein n=1 Tax=Symbiodinium sp. KB8 ... [more]
A0A7S1C2I6_9STRA2.340e-5953.33Hypothetical protein (Fragment) n=1 Tax=Bicosoecid... [more]
UPI00096B3A904.890e-4544.51ergothioneine biosynthesis protein 1-like isoform ... [more]
A0A496UZX4_9GAMM5.260e-4548.17FGE-sulfatase domain-containing protein n=1 Tax=Ga... [more]
A0A1X7UFB6_AMPQE1.100e-4444.51Uncharacterized protein n=2 Tax=Amphimedon queensl... [more]
UPI001C3F79352.440e-4343.46uncharacterized protein LOC121848036 n=1 Tax=Callo... [more]
A0A1S3K4W5_LINUN1.180e-4242.94uncharacterized protein LOC106178593 isoform X1 n=... [more]
UPI001B88E2C48.130e-4243.02LOW QUALITY PROTEIN: ergothioneine biosynthesis pr... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig95615contigM-pyrifera_M_contig95615:190..726 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score170.6
Seed ortholog evalue6.5e-40
Seed eggNOG ortholog400682.PAC_15715759
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Sulfatase-modifying factor enzyme 1
EggNOG OGs2QS9T@2759,38BFM@33154,3BJS2@33208,COG4301@1
COG Functional cat.S
Best tax levelMetazoa
Best eggNOG OGNA|NA|NA
Exons1
Model size537
Cds size537
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816870.4636314-CDS-M-pyrifera_M_contig95615:189..7261622816870.4636314-CDS-M-pyrifera_M_contig95615:189..726Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig95615 190..726 -
1692278227.0607278-CDS-M-pyrifera_M_contig95615:189..7261692278227.0607278-CDS-M-pyrifera_M_contig95615:189..726Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig95615 190..726 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig95615.22045.1prot_M-pyrifera_M_contig95615.22045.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig95615 190..726 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig95615.22045.1

>prot_M-pyrifera_M_contig95615.22045.1 ID=prot_M-pyrifera_M_contig95615.22045.1|Name=mRNA_M-pyrifera_M_contig95615.22045.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=179bp
ETTAVLGKPRDFPQFGFDNEYGKRVVCVPSFAASRFLVSNAEFLPFVLAG
GYRERKYWVSELGDDEGWRWVQYRNATHPSFWVATKHMPEYFGGTDDLPY
QKDDGSDLAGSGEEFKLRTEFHIVPMPWDWPVEVNVHESKAFLRWKAEQE
GTGVRYRLPTEAEHKVMRADPSPHPESTI
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mRNA from alignment at M-pyrifera_M_contig95615:190..726-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig95615.22045.1 ID=mRNA_M-pyrifera_M_contig95615.22045.1|Name=mRNA_M-pyrifera_M_contig95615.22045.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=537bp|location=Sequence derived from alignment at M-pyrifera_M_contig95615:190..726- (Macrocystis pyrifera P11B4 male)
GAGACGACAGCGGTATTGGGCAAGCCTCGAGATTTCCCACAATTCGGCTT CGACAACGAGTATGGGAAACGAGTGGTGTGCGTGCCGTCGTTTGCCGCCT CGCGGTTTCTCGTGTCCAACGCGGAGTTCCTGCCCTTTGTGCTGGCTGGA GGCTATCGCGAGCGCAAGTATTGGGTGTCTGAGCTTGGAGACGACGAAGG GTGGCGCTGGGTGCAGTATCGCAATGCCACGCACCCTTCGTTCTGGGTGG CCACAAAGCACATGCCCGAGTATTTCGGCGGAACCGATGATCTGCCGTAC CAAAAAGACGACGGGAGTGACTTGGCAGGATCGGGAGAAGAATTCAAGCT GCGCACGGAATTCCACATCGTTCCCATGCCGTGGGATTGGCCAGTGGAAG TGAACGTGCACGAATCCAAGGCGTTCTTGCGCTGGAAAGCGGAACAGGAA GGCACTGGAGTGCGATACCGGCTGCCCACTGAAGCCGAGCACAAGGTGAT GCGAGCAGATCCTTCGCCACACCCCGAATCCACCATC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig95615:190..726-

>mRNA_M-pyrifera_M_contig95615.22045.1 ID=mRNA_M-pyrifera_M_contig95615.22045.1|Name=mRNA_M-pyrifera_M_contig95615.22045.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1074bp|location=Sequence derived from alignment at M-pyrifera_M_contig95615:190..726- (Macrocystis pyrifera P11B4 male)
GAGACGACAGCGGTATTGGGCAAGCCTCGAGATTTCCCACAATTCGGCTT
CGACAACGAGTATGGGAAACGAGTGGTGTGCGTGCCGTCGTTTGCCGCCT
CGCGGTTTCTCGTGTCCAACGCGGAGTTCCTGCCCTTTGTGCTGGCTGGA
GGCTATCGCGAGCGCAAGTATTGGGTGTCTGAGCTTGGAGACGACGAAGG
GTGGCGCTGGGTGCAGTATCGCAATGCCACGCACCCTTCGTTCTGGGTGG
CCACAAAGCACATGCCCGAGTATTTCGGCGGAACCGATGATCTGCCGTAC
CAAAAAGACGACGGGAGTGACTTGGCAGGATCGGGAGAAGAATTCAAGCT
GCGCACGGAATTCCACATCGTTCCCATGCCGTGGGATTGGCCAGTGGAAG
TGAACGTGCACGAATCCAAGGCGTTCTTGCGCTGGAAAGCGGAACAGGAA
GGCACTGGAGTGCGATACCGGCTGCCCACTGAAGCCGAGCACAAGGTGAT
GCGAGCAGATCCTTCGCCACACCCCGAATCCACCATCGAGACGACAGCGG
TATTGGGCAAGCCTCGAGATTTCCCACAATTCGGCTTCGACAACGAGTAT
GGGAAACGAGTGGTGTGCGTGCCGTCGTTTGCCGCCTCGCGGTTTCTCGT
GTCCAACGCGGAGTTCCTGCCCTTTGTGCTGGCTGGAGGCTATCGCGAGC
GCAAGTATTGGGTGTCTGAGCTTGGAGACGACGAAGGGTGGCGCTGGGTG
CAGTATCGCAATGCCACGCACCCTTCGTTCTGGGTGGCCACAAAGCACAT
GCCCGAGTATTTCGGCGGAACCGATGATCTGCCGTACCAAAAAGACGACG
GGAGTGACTTGGCAGGATCGGGAGAAGAATTCAAGCTGCGCACGGAATTC
CACATCGTTCCCATGCCGTGGGATTGGCCAGTGGAAGTGAACGTGCACGA
ATCCAAGGCGTTCTTGCGCTGGAAAGCGGAACAGGAAGGCACTGGAGTGC
GATACCGGCTGCCCACTGAAGCCGAGCACAAGGTGATGCGAGCAGATCCT
TCGCCACACCCCGAATCCACCATC
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