mRNA_M-pyrifera_M_contig95514.22017.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig95514.22017.1
Unique NamemRNA_M-pyrifera_M_contig95514.22017.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig95514.22017.1 vs. uniprot
Match: A0A8J4V6E3_9MYCE (Signal peptidase complex subunit 2 n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4V6E3_9MYCE)

HSP 1 Score: 91.3 bits (225), Expect = 2.840e-20
Identity = 48/139 (34.53%), Postives = 83/139 (59.71%), Query Frame = 1
Query:   22 WKEDHSVSNLLLVLGYLGCALAAYSHLNPVPFPENKPILLACVVGYMVLSSAMTLLLSYVQKDTI-FKSKERHGRKLVVSTTLEKYDFAYKVRLEL--VRTGKSLTVDHRICESIASFFDEDGLLQKDRLCKVLDSSLK 429
            +K++H ++ + ++LG++GC LAA +   PVPFP+NKPIL+ CV  Y ++S+ +  +  ++QKD I F  KE + +++ VS+TL +YD  Y +++E   + T   +T      +SI  +FD  G   +D+    L    K
Sbjct:   40 YKQNHKMNYIKVLLGFIGCCLAALAQFYPVPFPQNKPILIVCVFFYAIISTILYYINMFIQKDYIVFAVKESNEKEIQVSSTLPRYDPNYTIKIEYRNISTPTPIT----FTKSIDQYFDIKGYFVQDKFFNDLSGVFK 174          
BLAST of mRNA_M-pyrifera_M_contig95514.22017.1 vs. uniprot
Match: UPI000644BAC5 (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI000644BAC5)

HSP 1 Score: 86.7 bits (213), Expect = 2.590e-18
Identity = 47/142 (33.10%), Postives = 88/142 (61.97%), Query Frame = 1
Query:    1 VQHFSKQ--WKEDHSVSNLLLVLGYLGCALAAYSHLNPVPFPENKPILLACVVGYMVLSSAMTLLLSYVQKDTIF--KSKERHGRKLVVSTTLEKYDFAYKVRLELVRTGKSLTVDHRICESIASFFDEDGLLQKDRLCKVL 414
            V++F+ +  +++ H+++ + ++LG +GC LA  +   P PFP+NKP+L+ CV+ YM++S A+  +  +VQKDTI   ++KE       +ST+L +++  Y ++ E   +GK +   H + + I ++F+  GLL +  L K L
Sbjct:   52 VRYFTSELSYEQVHTINYIKVLLGLVGCILAGLAQFYPEPFPKNKPVLIICVLLYMLISLALYYIAQFVQKDTILFGRAKESGNSDFRISTSLPRFEPNYTIKYE---SGKDIV--HSVTKPINNYFNTSGLLVEAPLFKDL 188          
BLAST of mRNA_M-pyrifera_M_contig95514.22017.1 vs. uniprot
Match: A0A7M7KYI2_VARDE (Signal peptidase complex subunit 2 n=1 Tax=Varroa destructor TaxID=109461 RepID=A0A7M7KYI2_VARDE)

HSP 1 Score: 75.9 bits (185), Expect = 3.120e-14
Identity = 46/139 (33.09%), Postives = 81/139 (58.27%), Query Frame = 1
Query:   25 KEDHSVSNLLLVLGYLGCALAAYSHLNPV--PFPENKPILLACVVGYMVLSSAMTLLLSYVQKD--TIFKSKERHG----RKLVVSTTLEKYDFAYKVRLELVRTGKSLTVDHRICESIASFFDEDGLLQKDRLCKVLD 417
            +EDHS+ +  L +  L  A A ++ L     PFPE++P+L+ACV+ Y  L   +TL  +Y +K    + K K+  G    +   V +TL+++D  Y++ + +   G+  T   ++ +SI+S+FD +G+L+KD L K +D
Sbjct:   41 EEDHSLPDGRLAISVLAVAAAGWALLWDWWHPFPESRPVLVACVLSYFALMGILTLYTNYYEKGIFVVAKRKDEVGLEAAKVFTVRSTLDRFDDMYRLEMTMHVEGQKSTSYAQLEKSISSWFDTNGVLRKDLLRKDVD 179          
BLAST of mRNA_M-pyrifera_M_contig95514.22017.1 vs. uniprot
Match: A0A1V9XW57_9ACAR (Signal peptidase complex subunit 2 n=1 Tax=Tropilaelaps mercedesae TaxID=418985 RepID=A0A1V9XW57_9ACAR)

HSP 1 Score: 72.8 bits (177), Expect = 4.660e-13
Identity = 45/142 (31.69%), Postives = 81/142 (57.04%), Query Frame = 1
Query:   13 SKQWK--EDHSVSNLLLVLGYLGCALAAYSHLNPV--PFPENKPILLACVVGYMVLSSAMTLLLSYVQKD--TIFKSKERHG----RKLVVSTTLEKYDFAYKVRLELVRTGKSLTVDHRICESIASFFDEDGLLQKDRLCK 408
            ++ W+  EDH + +  L +  L  A A ++ L     PFPE++P+L+ACV+ Y +L   +TL  +Y +K    + K K+  G    +   + + LE++D  Y++ + +   G+  T   ++ +SIAS+FD +G+L+KD L K
Sbjct:   35 TQDWRMEEDHRLPDGRLAISVLAVAAAGWALLWDWWHPFPESRPVLVACVLSYFLLMGILTLYTNYYEKGIFVVAKQKDEVGLEAAKVFTIRSFLERFDDMYRLEMTVNVEGQRKTGTAQLEKSIASWFDSNGVLRKDLLRK 176          
BLAST of mRNA_M-pyrifera_M_contig95514.22017.1 vs. uniprot
Match: UPI000D1CCFED (probable signal peptidase complex subunit 2 n=1 Tax=Selaginella moellendorffii TaxID=88036 RepID=UPI000D1CCFED)

HSP 1 Score: 65.1 bits (157), Expect = 3.590e-10
Identity = 39/126 (30.95%), Postives = 68/126 (53.97%), Query Frame = 1
Query:   22 WKEDHSVSNLLLVLGYLGCALAAYSHLNPVPFPENKPILLACVVGYMVLSSAMTLLLSYVQKDTIFKSKERHGR----KLVVSTTLEKYDFAYKVRLELVRTGK-SLTVDHRICESIASFFDEDGL 384
            +KE+ +VSN+ L LG+L C +A  + L P  FPENKP+LL C+  Y+VL+ A+  ++ + +K+ I  +    G      L +S+ L ++   Y +R+     G  S        +S+  +F  +G+
Sbjct:   38 FKENVTVSNMKLGLGFLACLVALAAQLYPKKFPENKPVLLVCIALYVVLNLAIQAIVYFKEKNNIVVTYPFPGSFTSTGLAISSKLPRHSPKYTLRIASADPGHISAHSPVEFTKSVTKWFSSEGV 163          
BLAST of mRNA_M-pyrifera_M_contig95514.22017.1 vs. uniprot
Match: A0A819CCM6_9BILA (Signal peptidase complex subunit 2 n=1 Tax=Rotaria sp. Silwood1 TaxID=2762511 RepID=A0A819CCM6_9BILA)

HSP 1 Score: 63.5 bits (153), Expect = 6.270e-10
Identity = 36/124 (29.03%), Postives = 65/124 (52.42%), Query Frame = 1
Query:   55 LVLGYLGCALAAYSHLNP--VPFPENKPILLACVVGYMVLSSAMTLLLSYVQKDTIFKSKE------RHGRKLVVSTTLEKYDFAYKVRLELVR-TGKSLTVDHRICESIASFFDEDGLLQKDR 399
            L +    C  + Y+ +     P P+++ +L+ CV+ Y +L S +TL + ++++   F  K         G + ++S+ L+KYD  Y + L+    T KS+     +  S+AS+FDE G+L  DR
Sbjct:    5 LYISLFACLFSIYALIYDWLYPIPKSRTVLIVCVLSYFILISILTLYMYFIERGRFFNGKLIDKTGLNSGSRCIISSKLKKYDDKYYLTLKYNESTNKSMGSSGVLISSVASYFDEKGVLCYDR 128          
BLAST of mRNA_M-pyrifera_M_contig95514.22017.1 vs. uniprot
Match: A0A4Z0Z761_9PEZI (Signal peptidase complex subunit 2 n=1 Tax=Xylaria hypoxylon TaxID=37992 RepID=A0A4Z0Z761_9PEZI)

HSP 1 Score: 63.2 bits (152), Expect = 3.160e-9
Identity = 42/126 (33.33%), Postives = 68/126 (53.97%), Query Frame = 1
Query:   13 SKQWKEDHSVSNLLLVLGYLGCALAAYSHL--NPVPFPENKPILLACVVGYMVLSSAMTLLLSYVQKDTIFKSKERHGRKLVVSTTLEKYDFAYKVRLELV-RTGKSLTVDHRICESIASFFDEDG 381
            S ++K+DHS+ +  L LGY   A+AA   L    + F + K    A V  Y V++ A+TL ++YV+K  +++ K   G K+ VST+ EK +  Y V +++  + G S  V+     S   +FD  G
Sbjct:   29 SLKFKQDHSLIDTRLALGYSAFAIAAACFLWDYKLGFEDTKWYTAAAVGLYSVINGALTLWITYVEKGAVYQGKAADGTKISVSTSTEKNEPTYHVVVDVTPKNGPSSKVE--FARSFTEWFDMQG 152          
BLAST of mRNA_M-pyrifera_M_contig95514.22017.1 vs. uniprot
Match: A0A1V1SUQ9_9FUNG (Signal peptidase complex subunit 2 n=2 Tax=Fungi TaxID=4751 RepID=A0A1V1SUQ9_9FUNG)

HSP 1 Score: 61.6 bits (148), Expect = 1.160e-8
Identity = 39/126 (30.95%), Postives = 69/126 (54.76%), Query Frame = 1
Query:   13 SKQWKEDHSVSNLLLVLGYLGCALAAYSHL--NPVPFPENKPILLACVVGYMVLSSAMTLLLSYVQKDTIFKSKERHGRKLVVSTTLEKYDFAYKVRLELV-RTGKSLTVDHRICESIASFFDEDG 381
            S ++K+DHS+ +  L LGY   A+AA   L    + F   K    A V  Y +++ A+TL ++YV+K T+++ + + G K+ VST+ +K +  Y V +++  + G S  ++     S   +FD  G
Sbjct:   29 SLKFKQDHSLVDTRLALGYGAFAIAAACFLWDYKLGFDSTKWFTAAAVALYSIVNGALTLWITYVEKGTVYRGEAKDGTKISVSTSTKKNEPTYYVVVDVAPKNGPSSKIE--FSRSFTEWFDVQG 152          
BLAST of mRNA_M-pyrifera_M_contig95514.22017.1 vs. uniprot
Match: A0A8J8T885_HALGN (Signal peptidase complex subunit 2 n=1 Tax=Halteria grandinella TaxID=5974 RepID=A0A8J8T885_HALGN)

HSP 1 Score: 61.2 bits (147), Expect = 1.450e-8
Identity = 26/70 (37.14%), Postives = 45/70 (64.29%), Query Frame = 1
Query:   16 KQWKEDHSVSNLLLVLGYLGCALAAYSHLNPVPFPENKPILLACVVGYMVLSSAMTLLLSYVQKDTIFKS 225
            K +KED+  ++L +++ ++ C L A SH  P+PFP+NK +L+ACV GY + +S   L+   ++ D  + S
Sbjct:   63 KGFKEDNRYTDLKILVAFIACVLGAVSHFYPIPFPQNKYLLMACVAGYAICASLYYLIERNLEGDHFYIS 132          
BLAST of mRNA_M-pyrifera_M_contig95514.22017.1 vs. uniprot
Match: A0A1B0APG9_9MUSC (Signal peptidase complex subunit 2 n=5 Tax=Glossina TaxID=7393 RepID=A0A1B0APG9_9MUSC)

HSP 1 Score: 60.1 bits (144), Expect = 2.760e-8
Identity = 42/144 (29.17%), Postives = 75/144 (52.08%), Query Frame = 1
Query:   25 KEDHSVSNLLLVLGYLGCALAAYSHLNPV--PFPENKPILLACVVGYMVLSSAMTLLLSYVQKDTIFKSKER--HGRKLV--VSTTLEKYDFAYKVRLELVRTGKSLTVDHRICESIASFFDEDGLLQKDRLCKVLDSSLKVIS 438
            KE   + N  L+L  L   +A  +H      PFPE+KP+L+ CVVGY  L   +TL  ++ +K T   + E+  +G + +   S+ ++KYD  Y + L  ++  KSL     + +S A+F D +G++  + +   ++     +S
Sbjct:   45 KEQFGLINTRLILCTLAVGVAVMAHGWDFTHPFPESKPVLMFCVVGYFALMGVLTLHTTFREKGTFAVATEKMPNGERRLWEASSDMKKYDDKYTLVLS-IKDAKSLKETSSV-KSCANFIDANGIVLDNHIANEVNRLYNTLS 186          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig95514.22017.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 24
Match NameE-valueIdentityDescription
A0A8J4V6E3_9MYCE2.840e-2034.53Signal peptidase complex subunit 2 n=1 Tax=Polysph... [more]
UPI000644BAC52.590e-1833.10hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
A0A7M7KYI2_VARDE3.120e-1433.09Signal peptidase complex subunit 2 n=1 Tax=Varroa ... [more]
A0A1V9XW57_9ACAR4.660e-1331.69Signal peptidase complex subunit 2 n=1 Tax=Tropila... [more]
UPI000D1CCFED3.590e-1030.95probable signal peptidase complex subunit 2 n=1 Ta... [more]
A0A819CCM6_9BILA6.270e-1029.03Signal peptidase complex subunit 2 n=1 Tax=Rotaria... [more]
A0A4Z0Z761_9PEZI3.160e-933.33Signal peptidase complex subunit 2 n=1 Tax=Xylaria... [more]
A0A1V1SUQ9_9FUNG1.160e-830.95Signal peptidase complex subunit 2 n=2 Tax=Fungi T... [more]
A0A8J8T885_HALGN1.450e-837.14Signal peptidase complex subunit 2 n=1 Tax=Halteri... [more]
A0A1B0APG9_9MUSC2.760e-829.17Signal peptidase complex subunit 2 n=5 Tax=Glossin... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig95514contigM-pyrifera_M_contig95514:100..540 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score77.4
Seed ortholog evalue6.2e-12
Seed eggNOG ortholog44689.DDB0237785
Preferred nameSPCS2
KEGG koko:K12947
KEGG Pathwayko03060,map03060
Hectar predicted targeting categorysignal peptide
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005787,GO:0005789,GO:0006465,GO:0006508,GO:0006518,GO:0006605,GO:0006807,GO:0006810,GO:0006886,GO:0008104,GO:0008150,GO:0008152,GO:0009987,GO:0010467,GO:0012505,GO:0015031,GO:0015833,GO:0016020,GO:0016485,GO:0019538,GO:0031984,GO:0032991,GO:0033036,GO:0033365,GO:0034613,GO:0034641,GO:0042175,GO:0042886,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043603,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0045047,GO:0045184,GO:0046907,GO:0051179,GO:0051234,GO:0051604,GO:0051641,GO:0051649,GO:0070727,GO:0070972,GO:0071702,GO:0071704,GO:0071705,GO:0072594,GO:0072599,GO:0098796,GO:0098827,GO:1901564,GO:1902494,GO:1905368
EggNOG free text desc.Microsomal signal peptidase 25 kDa subunit (SPC25)
EggNOG OGs3XHGH@554915,KOG4072@1,KOG4072@2759
COG Functional cat.U
Best tax levelAmoebozoa
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons1
Model size441
Cds size441
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816868.9328308-CDS-M-pyrifera_M_contig95514:99..5401622816868.9328308-CDS-M-pyrifera_M_contig95514:99..540Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig95514 100..540 -
1692278226.0499823-CDS-M-pyrifera_M_contig95514:99..5401692278226.0499823-CDS-M-pyrifera_M_contig95514:99..540Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig95514 100..540 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig95514.22017.1prot_M-pyrifera_M_contig95514.22017.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig95514 100..540 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig95514.22017.1

>prot_M-pyrifera_M_contig95514.22017.1 ID=prot_M-pyrifera_M_contig95514.22017.1|Name=mRNA_M-pyrifera_M_contig95514.22017.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=147bp
VQHFSKQWKEDHSVSNLLLVLGYLGCALAAYSHLNPVPFPENKPILLACV
VGYMVLSSAMTLLLSYVQKDTIFKSKERHGRKLVVSTTLEKYDFAYKVRL
ELVRTGKSLTVDHRICESIASFFDEDGLLQKDRLCKVLDSSLKVIS*
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mRNA from alignment at M-pyrifera_M_contig95514:100..540-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig95514.22017.1 ID=mRNA_M-pyrifera_M_contig95514.22017.1|Name=mRNA_M-pyrifera_M_contig95514.22017.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=441bp|location=Sequence derived from alignment at M-pyrifera_M_contig95514:100..540- (Macrocystis pyrifera P11B4 male)
GTGCAGCACTTCTCGAAGCAGTGGAAGGAGGACCACTCGGTGAGCAACCT GCTGCTGGTGCTGGGCTACCTGGGCTGTGCGCTGGCGGCGTACTCGCACC TCAACCCGGTGCCCTTCCCGGAGAACAAGCCGATCCTCCTGGCGTGCGTG GTGGGCTACATGGTGCTCAGCAGCGCGATGACGCTGCTGCTCTCCTATGT GCAGAAGGACACAATCTTCAAGTCGAAGGAGCGCCACGGCCGAAAGCTCG TCGTGAGCACCACGCTGGAGAAGTACGACTTTGCCTACAAGGTCCGTCTG GAGCTGGTCAGGACCGGTAAGAGCCTTACCGTCGACCACCGCATCTGCGA GAGCATTGCCTCCTTCTTCGACGAGGATGGCCTCCTGCAGAAGGACCGCC TCTGCAAGGTGCTCGACAGCTCCCTCAAGGTCATCTCTTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig95514:100..540-

>mRNA_M-pyrifera_M_contig95514.22017.1 ID=mRNA_M-pyrifera_M_contig95514.22017.1|Name=mRNA_M-pyrifera_M_contig95514.22017.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=882bp|location=Sequence derived from alignment at M-pyrifera_M_contig95514:100..540- (Macrocystis pyrifera P11B4 male)
GTGCAGCACTTCTCGAAGCAGTGGAAGGAGGACCACTCGGTGAGCAACCT
GCTGCTGGTGCTGGGCTACCTGGGCTGTGCGCTGGCGGCGTACTCGCACC
TCAACCCGGTGCCCTTCCCGGAGAACAAGCCGATCCTCCTGGCGTGCGTG
GTGGGCTACATGGTGCTCAGCAGCGCGATGACGCTGCTGCTCTCCTATGT
GCAGAAGGACACAATCTTCAAGTCGAAGGAGCGCCACGGCCGAAAGCTCG
TCGTGAGCACCACGCTGGAGAAGTACGACTTTGCCTACAAGGTCCGTCTG
GAGCTGGTCAGGACCGGTAAGAGCCTTACCGTCGACCACCGCATCTGCGA
GAGCATTGCCTCCTTCTTCGACGAGGATGGCCTCCTGCAGAAGGACCGCC
TCTGCAAGGTGCTCGACAGCTCCCTCAAGGTCATCTCTTGAGTGCAGCAC
TTCTCGAAGCAGTGGAAGGAGGACCACTCGGTGAGCAACCTGCTGCTGGT
GCTGGGCTACCTGGGCTGTGCGCTGGCGGCGTACTCGCACCTCAACCCGG
TGCCCTTCCCGGAGAACAAGCCGATCCTCCTGGCGTGCGTGGTGGGCTAC
ATGGTGCTCAGCAGCGCGATGACGCTGCTGCTCTCCTATGTGCAGAAGGA
CACAATCTTCAAGTCGAAGGAGCGCCACGGCCGAAAGCTCGTCGTGAGCA
CCACGCTGGAGAAGTACGACTTTGCCTACAAGGTCCGTCTGGAGCTGGTC
AGGACCGGTAAGAGCCTTACCGTCGACCACCGCATCTGCGAGAGCATTGC
CTCCTTCTTCGACGAGGATGGCCTCCTGCAGAAGGACCGCCTCTGCAAGG
TGCTCGACAGCTCCCTCAAGGTCATCTCTTGA
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