mRNA_M-pyrifera_M_contig94471.21808.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig94471.21808.1
Unique NamemRNA_M-pyrifera_M_contig94471.21808.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig94471.21808.1 vs. uniprot
Match: D8LXA8_BLAHO (HECT domain-containing protein n=1 Tax=Blastocystis hominis TaxID=12968 RepID=D8LXA8_BLAHO)

HSP 1 Score: 145 bits (365), Expect = 9.180e-38
Identity = 70/125 (56.00%), Postives = 94/125 (75.20%), Query Frame = 1
Query:    1 WEELELAVCGDAHVDVEVLKQNTVYIGGYSASHPVVRRFFTVLEEMDDEMRSKFVRFVWGRARLPKG-AWPRPFKLTRKSGSRGMLPIAHTCFFQLELPEYSSVSECRKRLLTALEYGLDGSMII 372
            WE+LE  V G   + VE+LK++TVYIG ++ SHPVV++F+ V+E + D+ RS+F+RF WGR++LPK  +WPRPFKLT K+    MLP+AHTCFFQLELP+YSS    R+RLL A+ YG  G  I+
Sbjct: 1001 WEQLERTVKGVPEISVELLKKHTVYIG-WTESHPVVKQFWNVMEGLTDKDRSQFLRFTWGRSKLPKSESWPRPFKLTYKNAGDYMLPVAHTCFFQLELPQYSSEQIMRERLLVAIHYGSSGEFIM 1124          
BLAST of mRNA_M-pyrifera_M_contig94471.21808.1 vs. uniprot
Match: UPI0007120857 (protein SSUH2-like protein n=1 Tax=Blastocystis sp. subtype 4 TaxID=944170 RepID=UPI0007120857)

HSP 1 Score: 141 bits (356), Expect = 1.550e-36
Identity = 66/124 (53.23%), Postives = 92/124 (74.19%), Query Frame = 1
Query:    1 WEELELAVCGDAHVDVEVLKQNTVYIGGYSASHPVVRRFFTVLEEMDDEMRSKFVRFVWGRARLP-KGAWPRPFKLTRKSGSRGMLPIAHTCFFQLELPEYSSVSECRKRLLTALEYGLDGSMI 369
            W++LE AVCG+  +D+E LKQ+T+Y   ++  HPVV+RF+ V+E +D++ RS+F+RF WGR+RLP K  WPRPFKL  ++    MLP+AHTCFF+LELP+YSS    R+RLL A+ YG    M+
Sbjct: 2614 WDQLERAVCGEPEIDIEDLKQHTIY-SNWTEQHPVVKRFWKVMEGLDNKDRSQFLRFTWGRSRLPHKDNWPRPFKLCARNYGDDMLPLAHTCFFELELPQYSSEKIMRERLLIAIHYGASEFML 2736          
BLAST of mRNA_M-pyrifera_M_contig94471.21808.1 vs. uniprot
Match: A0A196S4B3_BLAHN (HECT E3 ubiquitin ligase n=1 Tax=Blastocystis sp. subtype 1 (strain ATCC 50177 / NandII) TaxID=478820 RepID=A0A196S4B3_BLAHN)

HSP 1 Score: 139 bits (350), Expect = 9.990e-36
Identity = 70/125 (56.00%), Postives = 91/125 (72.80%), Query Frame = 1
Query:    1 WEELELAVCGDAHVDVEVLKQNTVYIGGYSASHPVVRRFFTVLEEMDDEMRSKFVRFVWGRARLPKG-AWPRPFKLTRKSGSRGMLPIAHTCFFQLELPEYSSVSECRKRLLTALEYGLDGSMII 372
            WE+LE AV G   + VE LK++TVYIG ++ SHPVV+RF+ V+ ++ D+ RS+F+RF WGR++LPK   WPR FKLT K+    MLPIAHTCFFQLELP+YS+    ++RLL A  YG  G  II
Sbjct: 2717 WEQLERAVKGVPEISVEELKKHTVYIG-WTESHPVVQRFWRVMAQLTDKDRSQFLRFSWGRSKLPKSDPWPRQFKLTYKNAGDEMLPIAHTCFFQLELPQYSTDQIMKERLLVAFNYGSSGEFII 2840          
BLAST of mRNA_M-pyrifera_M_contig94471.21808.1 vs. uniprot
Match: A0A7S0K3J3_CAFRO (Hypothetical protein n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A7S0K3J3_CAFRO)

HSP 1 Score: 137 bits (346), Expect = 1.300e-35
Identity = 66/124 (53.23%), Postives = 89/124 (71.77%), Query Frame = 1
Query:    1 WEELELAVCGDAHVDVEVLKQNTVYIGGYSASHPVVRRFFTVLEEMDDEMRSKFVRFVWGRARLPKGA-WPRPFKLTRKSGSRGMLPIAHTCFFQLELPEYSSVSECRKRLLTALEYGLDGSMI 369
            W ELE+ VCGDA VDV+ L ++TV   GY  +   +R  F  L  M  + RS+F+RFVWGR+RLP+G+ WPRPFK++R  G    LP+AH+CFFQLELP YSS +  R+RL+TAL+YG+D  ++
Sbjct:  456 WSELEVFVCGDARVDVDALAEHTV-CEGYGPNDRPLRLLFRCLRGMSQQDRSRFIRFVWGRSRLPRGSRWPRPFKVSRHFGGDHSLPVAHSCFFQLELPPYSSEAVLRRRLMTALDYGMDSFLL 578          
BLAST of mRNA_M-pyrifera_M_contig94471.21808.1 vs. uniprot
Match: A0A5A8CWI7_CAFRO (HECT domain-containing protein n=5 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CWI7_CAFRO)

HSP 1 Score: 137 bits (346), Expect = 3.350e-35
Identity = 66/124 (53.23%), Postives = 89/124 (71.77%), Query Frame = 1
Query:    1 WEELELAVCGDAHVDVEVLKQNTVYIGGYSASHPVVRRFFTVLEEMDDEMRSKFVRFVWGRARLPKGA-WPRPFKLTRKSGSRGMLPIAHTCFFQLELPEYSSVSECRKRLLTALEYGLDGSMI 369
            W ELE+ VCGDA VDV+ L ++TV   GY  +   +R  F  L  M  + RS+F+RFVWGR+RLP+G+ WPRPFK++R  G    LP+AH+CFFQLELP YSS +  R+RL+TAL+YG+D  ++
Sbjct: 1086 WSELEVFVCGDARVDVDALAEHTV-CEGYGPNDRPLRLLFRCLRGMSQQDRSRFIRFVWGRSRLPRGSRWPRPFKVSRHFGGDHSLPVAHSCFFQLELPPYSSEAVLRRRLMTALDYGMDSFLL 1208          
BLAST of mRNA_M-pyrifera_M_contig94471.21808.1 vs. uniprot
Match: D8M1C1_BLAHO (HECT domain-containing protein n=1 Tax=Blastocystis hominis TaxID=12968 RepID=D8M1C1_BLAHO)

HSP 1 Score: 137 bits (346), Expect = 3.440e-35
Identity = 66/117 (56.41%), Postives = 86/117 (73.50%), Query Frame = 1
Query:    1 WEELELAVCGDAHVDVEVLKQNTVYIGGYSASHPVVRRFFTVLEEMDDEMRSKFVRFVWGRARLPKGAWPRPFKLTRKSGSRGMLPIAHTCFFQLELPEYSSVSECRKRLLTALEYG 351
            WE+LE  V G   V VEVLKQ+TVYIG ++  H VV+RF+ V++E+ ++ RS F+RFVWGR+RLPK  WPRPFKL         LPIAHTCFFQL+LP+YS+ +  R+RLL A+ +G
Sbjct: 2179 WEQLERTVQGVPEVSVEVLKQHTVYIG-WTEEHEVVQRFWRVMKELGNKDRSSFLRFVWGRSRLPKVVWPRPFKLNHSEAGNDRLPIAHTCFFQLDLPQYSTDAIMRERLLVAIHFG 2294          
BLAST of mRNA_M-pyrifera_M_contig94471.21808.1 vs. uniprot
Match: A0A662WQB5_9STRA (HECT domain-containing protein (Fragment) n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662WQB5_9STRA)

HSP 1 Score: 127 bits (319), Expect = 7.210e-35
Identity = 59/125 (47.20%), Postives = 84/125 (67.20%), Query Frame = 1
Query:    1 WEELELAVCGDAHVDVEVLKQNTVYIGGYSASHPVVRRFFTVLEEMDDEMRSKFVRFVWGRARLPKGAWPRPFKLTRKSG--SRGMLPIAHTCFFQLELPEYSSVSECRKRLLTALEYGLDGSMI 369
            W+ELEL  CG   +D+ + +++T Y  GY+   P VR F+ VL +  DE R+ FVRF WGR+RLP+G WP+PFKL++K G  +   LP+AHTCFF +ELP Y++    R RLL  + +GL G ++
Sbjct:   48 WQELELLTCGSPKIDLALWQRHTRY-DGYAEDDPTVRLFWEVLGDFSDEQRTDFVRFAWGRSRLPRGKWPQPFKLSKKGGRDATHSLPVAHTCFFSVELPPYTTREIMRSRLLATITFGLGGILM 171          
BLAST of mRNA_M-pyrifera_M_contig94471.21808.1 vs. uniprot
Match: UPI00071159D3 (HECT E3 ubiquitin n=1 Tax=Blastocystis sp. subtype 4 TaxID=944170 RepID=UPI00071159D3)

HSP 1 Score: 136 bits (343), Expect = 8.390e-35
Identity = 64/125 (51.20%), Postives = 91/125 (72.80%), Query Frame = 1
Query:    1 WEELELAVCGDAHVDVEVLKQNTVYIGGYSASHPVVRRFFTVLEEMDDEMRSKFVRFVWGRARLPKGA-WPRPFKLTRKSGSRGMLPIAHTCFFQLELPEYSSVSECRKRLLTALEYGLDGSMII 372
            WE+LE +V G+  + V+VL+++TVY+G ++  HPVV RF+ V+ ++ D+ RS+F+RF WGR+RLPK   W RPFKLT K+    MLPIAHTCFFQLELP+Y++      RLL A+ YG+ G  ++
Sbjct:  872 WEQLERSVKGEPEISVDVLRRHTVYLG-WTEHHPVVERFWNVMSQLTDKDRSQFLRFAWGRSRLPKEEPWSRPFKLTYKNAGDEMLPIAHTCFFQLELPQYTTDKVMHDRLLVAINYGVSGEFML 995          
BLAST of mRNA_M-pyrifera_M_contig94471.21808.1 vs. uniprot
Match: A0A196SMH9_BLAHN (HECT E3 ubiquitin ligase n=1 Tax=Blastocystis sp. subtype 1 (strain ATCC 50177 / NandII) TaxID=478820 RepID=A0A196SMH9_BLAHN)

HSP 1 Score: 136 bits (343), Expect = 8.790e-35
Identity = 64/124 (51.61%), Postives = 90/124 (72.58%), Query Frame = 1
Query:    1 WEELELAVCGDAHVDVEVLKQNTVYIGGYSASHPVVRRFFTVLEEMDDEMRSKFVRFVWGRARLP-KGAWPRPFKLTRKSGSRGMLPIAHTCFFQLELPEYSSVSECRKRLLTALEYGLDGSMI 369
            W +LE AVCG+  +D+E LK +T+Y   ++  HPVV+RF+ V+E +D++ RS+F+RF WGR+RLP K  WPRPFKL  ++    MLP+AHTCFF+LELP+YS+    R+RLL A+ YG    M+
Sbjct: 2899 WNQLERAVCGEPEIDIEDLKLHTIYTN-WTEQHPVVKRFWKVMEGLDNKDRSQFLRFTWGRSRLPHKDNWPRPFKLVARNYGDDMLPLAHTCFFELELPQYSTDQIMRERLLIAIHYGASEFML 3021          
BLAST of mRNA_M-pyrifera_M_contig94471.21808.1 vs. uniprot
Match: A0A196SH75_BLAHN (HECT E3 ubiquitin ligase n=1 Tax=Blastocystis sp. subtype 1 (strain ATCC 50177 / NandII) TaxID=478820 RepID=A0A196SH75_BLAHN)

HSP 1 Score: 134 bits (336), Expect = 7.740e-34
Identity = 64/118 (54.24%), Postives = 90/118 (76.27%), Query Frame = 1
Query:    1 WEELELAVCGDAHVDVEVLKQNTVYIGGYSASHPVVRRFFTVLEEMDDEMRSKFVRFVWGRARLPKGA-WPRPFKLTRKSGSRGMLPIAHTCFFQLELPEYSSVSECRKRLLTALEYG 351
            WE+LE AV G   + VE LK++TVYIG +S SHPVV+RF+ V+ ++ ++ RS+F+RF WGR+RLPK + WPRPFKLT       +LP++HTCFFQLELP+YS+ +  ++RLL A+ +G
Sbjct: 3494 WEQLERAVKGVPEISVEELKKHTVYIG-WSESHPVVQRFWRVMAQLSNKDRSQFLRFAWGRSRLPKTSNWPRPFKLTYSKAGDELLPVSHTCFFQLELPQYSTDAIMKERLLVAIHFG 3610          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig94471.21808.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LXA8_BLAHO9.180e-3856.00HECT domain-containing protein n=1 Tax=Blastocysti... [more]
UPI00071208571.550e-3653.23protein SSUH2-like protein n=1 Tax=Blastocystis sp... [more]
A0A196S4B3_BLAHN9.990e-3656.00HECT E3 ubiquitin ligase n=1 Tax=Blastocystis sp. ... [more]
A0A7S0K3J3_CAFRO1.300e-3553.23Hypothetical protein n=1 Tax=Cafeteria roenbergens... [more]
A0A5A8CWI7_CAFRO3.350e-3553.23HECT domain-containing protein n=5 Tax=Cafeteria r... [more]
D8M1C1_BLAHO3.440e-3556.41HECT domain-containing protein n=1 Tax=Blastocysti... [more]
A0A662WQB5_9STRA7.210e-3547.20HECT domain-containing protein (Fragment) n=2 Tax=... [more]
UPI00071159D38.390e-3551.20HECT E3 ubiquitin n=1 Tax=Blastocystis sp. subtype... [more]
A0A196SMH9_BLAHN8.790e-3551.61HECT E3 ubiquitin ligase n=1 Tax=Blastocystis sp. ... [more]
A0A196SH75_BLAHN7.740e-3454.24HECT E3 ubiquitin ligase n=1 Tax=Blastocystis sp. ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig94471contigM-pyrifera_M_contig94471:365..742 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score130.6
Seed ortholog evalue5.3e-28
Seed eggNOG ortholog65071.PYU1_T013085
KEGG rclassRC00017
KEGG koko:K05288
KEGG ReactionR05923,R08107
KEGG Pathwayko00563,ko01100,map00563,map01100
Hectar predicted targeting categoryother localisation
EggNOG free text desc.HECT E3 ubiquitin ligase. Source PGD
EggNOG OGs1MDRJ@121069,COG5184@1,KOG1426@2759
COG Functional cat.DZ
Best tax levelPythiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001
Exons1
Model size378
Cds size378
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816858.5279198-CDS-M-pyrifera_M_contig94471:364..7421622816858.5279198-CDS-M-pyrifera_M_contig94471:364..742Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig94471 365..742 -
1692278220.0256689-CDS-M-pyrifera_M_contig94471:364..7421692278220.0256689-CDS-M-pyrifera_M_contig94471:364..742Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig94471 365..742 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig94471.21808.1prot_M-pyrifera_M_contig94471.21808.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig94471 365..742 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig94471.21808.1

>prot_M-pyrifera_M_contig94471.21808.1 ID=prot_M-pyrifera_M_contig94471.21808.1|Name=mRNA_M-pyrifera_M_contig94471.21808.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=126bp
WEELELAVCGDAHVDVEVLKQNTVYIGGYSASHPVVRRFFTVLEEMDDEM
RSKFVRFVWGRARLPKGAWPRPFKLTRKSGSRGMLPIAHTCFFQLELPEY
SSVSECRKRLLTALEYGLDGSMIIA*
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mRNA from alignment at M-pyrifera_M_contig94471:365..742-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig94471.21808.1 ID=mRNA_M-pyrifera_M_contig94471.21808.1|Name=mRNA_M-pyrifera_M_contig94471.21808.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=378bp|location=Sequence derived from alignment at M-pyrifera_M_contig94471:365..742- (Macrocystis pyrifera P11B4 male)
TGGGAAGAGTTAGAATTGGCCGTGTGTGGAGATGCACATGTGGATGTTGA AGTGTTGAAGCAAAACACAGTCTACATTGGCGGATATTCTGCTTCACACC CAGTTGTTCGTCGCTTCTTCACTGTCCTTGAAGAGATGGACGATGAAATG CGGTCCAAATTCGTCCGCTTCGTCTGGGGAAGAGCAAGACTCCCCAAAGG AGCGTGGCCAAGACCATTCAAGCTCACAAGAAAGTCTGGAAGTAGAGGAA TGCTCCCCATTGCACACACGTGCTTCTTCCAGCTTGAACTCCCAGAATAT TCCTCTGTGTCTGAATGCAGGAAACGCCTCCTCACAGCTCTAGAATATGG CCTAGATGGCTCCATGATTATCGCATAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig94471:365..742-

>mRNA_M-pyrifera_M_contig94471.21808.1 ID=mRNA_M-pyrifera_M_contig94471.21808.1|Name=mRNA_M-pyrifera_M_contig94471.21808.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=756bp|location=Sequence derived from alignment at M-pyrifera_M_contig94471:365..742- (Macrocystis pyrifera P11B4 male)
TGGGAAGAGTTAGAATTGGCCGTGTGTGGAGATGCACATGTGGATGTTGA
AGTGTTGAAGCAAAACACAGTCTACATTGGCGGATATTCTGCTTCACACC
CAGTTGTTCGTCGCTTCTTCACTGTCCTTGAAGAGATGGACGATGAAATG
CGGTCCAAATTCGTCCGCTTCGTCTGGGGAAGAGCAAGACTCCCCAAAGG
AGCGTGGCCAAGACCATTCAAGCTCACAAGAAAGTCTGGAAGTAGAGGAA
TGCTCCCCATTGCACACACGTGCTTCTTCCAGCTTGAACTCCCAGAATAT
TCCTCTGTGTCTGAATGCAGGAAACGCCTCCTCACAGCTCTAGAATATGG
CCTAGATGGCTCCATGATTATCGCATAGTGGGAAGAGTTAGAATTGGCCG
TGTGTGGAGATGCACATGTGGATGTTGAAGTGTTGAAGCAAAACACAGTC
TACATTGGCGGATATTCTGCTTCACACCCAGTTGTTCGTCGCTTCTTCAC
TGTCCTTGAAGAGATGGACGATGAAATGCGGTCCAAATTCGTCCGCTTCG
TCTGGGGAAGAGCAAGACTCCCCAAAGGAGCGTGGCCAAGACCATTCAAG
CTCACAAGAAAGTCTGGAAGTAGAGGAATGCTCCCCATTGCACACACGTG
CTTCTTCCAGCTTGAACTCCCAGAATATTCCTCTGTGTCTGAATGCAGGA
AACGCCTCCTCACAGCTCTAGAATATGGCCTAGATGGCTCCATGATTATC
GCATAG
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