mRNA_M-pyrifera_M_contig93829.21664.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig93829.21664.1
Unique NamemRNA_M-pyrifera_M_contig93829.21664.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig93829.21664.1 vs. uniprot
Match: A0A813AQX0_9DINO (MhcA protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813AQX0_9DINO)

HSP 1 Score: 202 bits (514), Expect = 3.350e-62
Identity = 106/155 (68.39%), Postives = 119/155 (76.77%), Query Frame = 1
Query:    1 NMVKLNDLNEAAILYNLRMRFKADTIYTAVGSILVSVNPFKLLPLYTPQVIDKYIERGARDLPPHVFGIADFAYRSMLDTSVSQSVVISGESGAGKTEAMKLILQYLAEVSGRTSGVKPGSVDASESLEQQILKANPVMEAFGNAKTTRNNNSSR 465
            NMVKLNDLNE  IL+NLR RFK D IYT V SIL++VNPFK + LYTP+V+D Y + GAR  PPH+F +AD AY  ML     Q+VVISGESGAGKTE MKL LQY+AEVSGR    K G     ESLEQQILK+NP+MEAFGNAKT RNNNSSR
Sbjct:   11 NMVKLNDLNEPVILHNLRQRFKKDIIYTYVSSILIAVNPFKQMTLYTPEVMDTYKDGGARVQPPHIFAVADNAYNGMLADQKDQAVVISGESGAGKTETMKLTLQYIAEVSGRAGKAKGGE----ESLEQQILKSNPIMEAFGNAKTVRNNNSSR 161          
BLAST of mRNA_M-pyrifera_M_contig93829.21664.1 vs. uniprot
Match: A0A7S0JX54_CAFRO (Hypothetical protein (Fragment) n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A7S0JX54_CAFRO)

HSP 1 Score: 211 bits (538), Expect = 1.490e-60
Identity = 106/156 (67.95%), Postives = 130/156 (83.33%), Query Frame = 1
Query:    1 NMVKLNDLNEAAILYNLRMRFKADTIYTAVGSILVSVNPFKLLPLYTPQVIDKYIERGARDLPPHVFGIADFAYRSMLDTSVSQSVVISGESGAGKTEAMKLILQYLAEVSGRTSGVKPGSV-DASESLEQQILKANPVMEAFGNAKTTRNNNSSR 465
            NMVK+N+LNE  I++NLR RFK+  IYT +G+IL++VNPF+LLP+YTP+++DKY + G R  PPH++ IADFAY+ M+  S SQSVVISGESGAGKTE MKL+LQ++AE SGR +  K GS  D  ESLEQQILK+NPVMEAFGNAKTTRNNNSSR
Sbjct:   67 NMVKMNNLNEPVIMHNLRSRFKSKKIYTYIGNILIAVNPFELLPIYTPEILDKYKDGGGRSQPPHIYAIADFAYQGMIAESASQSVVISGESGAGKTETMKLVLQFIAEASGRAA--KQGSSGDKQESLEQQILKSNPVMEAFGNAKTTRNNNSSR 220          
BLAST of mRNA_M-pyrifera_M_contig93829.21664.1 vs. uniprot
Match: A0A5A8CSV2_CAFRO (Uncharacterized protein n=3 Tax=Sar TaxID=2698737 RepID=A0A5A8CSV2_CAFRO)

HSP 1 Score: 211 bits (538), Expect = 1.510e-60
Identity = 106/156 (67.95%), Postives = 130/156 (83.33%), Query Frame = 1
Query:    1 NMVKLNDLNEAAILYNLRMRFKADTIYTAVGSILVSVNPFKLLPLYTPQVIDKYIERGARDLPPHVFGIADFAYRSMLDTSVSQSVVISGESGAGKTEAMKLILQYLAEVSGRTSGVKPGSV-DASESLEQQILKANPVMEAFGNAKTTRNNNSSR 465
            NMVK+N+LNE  I++NLR RFK+  IYT +G+IL++VNPF+LLP+YTP+++DKY + G R  PPH++ IADFAY+ M+  S SQSVVISGESGAGKTE MKL+LQ++AE SGR +  K GS  D  ESLEQQILK+NPVMEAFGNAKTTRNNNSSR
Sbjct:   67 NMVKMNNLNEPVIMHNLRSRFKSKKIYTYIGNILIAVNPFELLPIYTPEILDKYKDGGGRSQPPHIYAIADFAYQGMIAESASQSVVISGESGAGKTETMKLVLQFIAEASGRAA--KQGSSGDKQESLEQQILKSNPVMEAFGNAKTTRNNNSSR 220          
BLAST of mRNA_M-pyrifera_M_contig93829.21664.1 vs. uniprot
Match: A0A5A8CB23_CAFRO (Uncharacterized protein n=3 Tax=Sar TaxID=2698737 RepID=A0A5A8CB23_CAFRO)

HSP 1 Score: 211 bits (538), Expect = 1.510e-60
Identity = 106/155 (68.39%), Postives = 126/155 (81.29%), Query Frame = 1
Query:    1 NMVKLNDLNEAAILYNLRMRFKADTIYTAVGSILVSVNPFKLLPLYTPQVIDKYIERGARDLPPHVFGIADFAYRSMLDTSVSQSVVISGESGAGKTEAMKLILQYLAEVSGRTSGVKPGSVDASESLEQQILKANPVMEAFGNAKTTRNNNSSR 465
            NMVK+N+LNE  IL+NLR RFKA  IYT +G+IL++VNPF+LL +YTP ++DKY + G R  PPH++ IADFAY+ M+    SQSVVISGESGAGKTE MKL+LQ+LAE SGR SG K G     +SLEQQILK+NPVMEAFGNAKTTRNNNSSR
Sbjct:   67 NMVKMNNLNEPVILHNLRARFKAKKIYTYIGNILIAVNPFELLAIYTPDILDKYKDGGGRSQPPHIYAIADFAYQGMIADGASQSVVISGESGAGKTETMKLVLQFLAEASGRASG-KSGDGTKQDSLEQQILKSNPVMEAFGNAKTTRNNNSSR 220          
BLAST of mRNA_M-pyrifera_M_contig93829.21664.1 vs. uniprot
Match: A0A7S2W799_9STRA (Hypothetical protein (Fragment) n=1 Tax=labyrinthulid quahog parasite QPX TaxID=96639 RepID=A0A7S2W799_9STRA)

HSP 1 Score: 183 bits (465), Expect = 2.260e-56
Identity = 92/149 (61.74%), Postives = 118/149 (79.19%), Query Frame = 1
Query:    1 NMVKLNDLNEAAILYNLRMRFKADTIYTAVGSILVSVNPFKLLPLYTPQVIDKYIERGARDLPPHVFGIADFAYRSMLDTSVSQSVVISGESGAGKTEAMKLILQYLAEVSGRTSGVKPGSVDASE--SLEQQILKANPVMEAFGNAKT 441
            NMV+L +LNEA+IL+NLR+RF+ D IYT VG+ILVSVNPFK+LPLYT ++++KY   G RDLPPHV+G+AD AY+SM+     Q+ ++SGESGAGKTEA K+ LQY+AEVSG +         + E  SL++QILKANP+MEAFGNAKT
Sbjct:   36 NMVQLKELNEASILHNLRLRFENDKIYTNVGTILVSVNPFKMLPLYTSEMLEKYKAEGYRDLPPHVYGVADDAYKSMVSGKQDQACIVSGESGAGKTEATKIFLQYIAEVSGASLAKDTDEQVSFEDASLQEQILKANPLMEAFGNAKT 184          
BLAST of mRNA_M-pyrifera_M_contig93829.21664.1 vs. uniprot
Match: A0A812RZ32_SYMMI (MhcA protein n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812RZ32_SYMMI)

HSP 1 Score: 183 bits (465), Expect = 2.580e-55
Identity = 99/146 (67.81%), Postives = 118/146 (80.82%), Query Frame = 1
Query:   34 AILYNLRMRFKADTIYTAVGSILVSVNPFKLLPLYTPQVIDKYIERGARDLPPHVFGIADFAYRSMLDTSVSQSVVISGESGAGKTEAMKLILQYLAEVSGRTSGV--KPGSVDASESLEQQILKANPVMEAFGNAKTTRNNNSSR 465
            +IL+NLR+R+K D IYT+V SIL+SVNPFKLLPLYTP+++D Y   G+RD PPHVF +A  AY SML  S  QSVVISGESGAGK+EA KLILQ+L +VS R +G   KPGS ++S  LEQQIL ANP++EAFGNAKT RNNNSSR
Sbjct:    2 SILHNLRIRYKEDRIYTSVSSILISVNPFKLLPLYTPEILDLY-RTGSRDKPPHVFAVAYNAYASMLTDSGDQSVVISGESGAGKSEATKLILQFLTDVSSRQTGAERKPGSGNSSN-LEQQILAANPILEAFGNAKTLRNNNSSR 145          
BLAST of mRNA_M-pyrifera_M_contig93829.21664.1 vs. uniprot
Match: A0A7S0XE81_9STRA (Hypothetical protein (Fragment) n=1 Tax=Chromulina nebulosa TaxID=96789 RepID=A0A7S0XE81_9STRA)

HSP 1 Score: 182 bits (461), Expect = 8.730e-55
Identity = 97/155 (62.58%), Postives = 119/155 (76.77%), Query Frame = 1
Query:    1 NMVKLNDLNEAAILYNLRMRFKADTIYTAVGSILVSVNPFKLLPLYTPQVIDKYIERGARDLPPHVFGIADFAYRSMLDTSVSQSVVISGESGAGKTEAMKLILQYLAEVSGRTSGVKPGSVDASESLEQQILKANPVMEAFGNAKTTRNNNSSR 465
            ++V ++DLNE +IL+NLR+R+K D IYT + SIL+SVNPFKLLPLYTP+V+D Y   G R   PHVFGIA  A+  ML+ S  QSVVISGESGAGK+EA KLILQ+L +VS + SG       + +SLEQQIL ANP++EA GNAKT RNNNSSR
Sbjct:    4 DLVNISDLNEMSILHNLRIRYKEDKIYTNISSILISVNPFKLLPLYTPEVLDSY-RSGYRGKAPHVFGIAFNAFHDMLNESRDQSVVISGESGAGKSEATKLILQFLTDVSSKASG-------SQQSLEQQILAANPILEALGNAKTLRNNNSSR 150          
BLAST of mRNA_M-pyrifera_M_contig93829.21664.1 vs. uniprot
Match: A0A7S3PSH7_9STRA (Hypothetical protein (Fragment) n=1 Tax=Aplanochytrium stocchinoi TaxID=215587 RepID=A0A7S3PSH7_9STRA)

HSP 1 Score: 181 bits (459), Expect = 1.470e-54
Identity = 96/169 (56.80%), Postives = 123/169 (72.78%), Query Frame = 1
Query:    1 NMVKLNDLNEAAILYNLRMRFKADTIYTAVGSILVSVNPFKLLPLYTPQVIDKYIERGARDLPPHVFGIADFAYRSMLDTSVSQSVVISGESGAGKTEAMKLILQYLAEVSGRTSGVKPGSVDASE--------------SLEQQILKANPVMEAFGNAKTTRNNNSSR 465
            NMV+L +LNEA+IL+NLR+RF A+ IYT VG+ILVSVNPFKLL +YT +++ KY ++G RDLPPHV+G+AD AY  M     +QS ++SGESGAGKTEA K+ LQ++AEVS  T+    G  +                 +L++QILKANP+MEAFGNAKT RNNNSSR
Sbjct:   68 NMVQLKELNEASILHNLRIRFDANNIYTNVGTILVSVNPFKLLNIYTTEILSKYRDQGFRDLPPHVYGVADAAYSQMTSFGNNQSCIVSGESGAGKTEATKIFLQFIAEVSS-TAANPSGKAEXXXXXXXXXXXXXXDKVTLQEQILKANPLMEAFGNAKTLRNNNSSR 235          
BLAST of mRNA_M-pyrifera_M_contig93829.21664.1 vs. uniprot
Match: A0A7R9YCN6_9STRA (Hypothetical protein (Fragment) n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9YCN6_9STRA)

HSP 1 Score: 192 bits (489), Expect = 2.410e-54
Identity = 99/155 (63.87%), Postives = 125/155 (80.65%), Query Frame = 1
Query:    1 NMVKLNDLNEAAILYNLRMRFKADTIYTAVGSILVSVNPFKLLPLYTPQVIDKYIERGARDLPPHVFGIADFAYRSMLDTSVSQSVVISGESGAGKTEAMKLILQYLAEVSGRTSGVKPGSVDASESLEQQILKANPVMEAFGNAKTTRNNNSSR 465
            N++ L DLNE AIL+NLR+R+K D IYT+V SIL+SVNPFKLLPLYTP+VID Y E G R  PPHVFGIA  A+  ML  +++QSV++SGESGAGK+EA KL+LQ++AEVS + + V  G    + +LEQQ+L+ANP+ME+FGNAKT RNNNSSR
Sbjct:   94 NLIHLRDLNEHAILHNLRIRYKEDKIYTSVSSILISVNPFKLLPLYTPEVIDDYRE-GIRGKPPHVFGIAWAAHTQMLSDNINQSVIVSGESGAGKSEAAKLMLQFIAEVSNKAAKVS-GKAAVTSNLEQQLLQANPIMESFGNAKTLRNNNSSR 246          
BLAST of mRNA_M-pyrifera_M_contig93829.21664.1 vs. uniprot
Match: A0A482S4P7_9ARCH (Myosin motor domain-containing protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482S4P7_9ARCH)

HSP 1 Score: 179 bits (454), Expect = 6.600e-53
Identity = 94/156 (60.26%), Postives = 120/156 (76.92%), Query Frame = 1
Query:    1 NMVKLNDLNEAAILYNLRMRFKADTIYTAVGSILVSVNPFKLLPLYTPQVIDKYIERGARDLPPHVFGIADFAYRSMLDTSVSQSVVISGESGAGKTEAMKLILQYLAEVSGRTSGVK-PGSVDASESLEQQILKANPVMEAFGNAKTTRNNNSSR 465
            ++V ++DLNE +IL+NLR+RFK D IYT + SIL+SVNPFKLLPLYTP++++ Y   G+R  PPHVF IA  +Y +ML  ++ QSVVISGESGAGK+EA KLILQ+L +VS + +     G+   S  LE QIL ANP++EAFGNAKT RNNNSSR
Sbjct:   33 DLVNISDLNEMSILHNLRIRFKEDRIYTNISSILISVNPFKLLPLYTPEILEMY-RTGSRGKPPHVFAIAVNSYNNMLADALDQSVVISGESGAGKSEATKLILQFLTDVSHKAATTTHTGASQVSNVLEAQILAANPILEAFGNAKTLRNNNSSR 187          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig93829.21664.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A813AQX0_9DINO3.350e-6268.39MhcA protein n=1 Tax=Symbiodinium sp. KB8 TaxID=23... [more]
A0A7S0JX54_CAFRO1.490e-6067.95Hypothetical protein (Fragment) n=1 Tax=Cafeteria ... [more]
A0A5A8CSV2_CAFRO1.510e-6067.95Uncharacterized protein n=3 Tax=Sar TaxID=2698737 ... [more]
A0A5A8CB23_CAFRO1.510e-6068.39Uncharacterized protein n=3 Tax=Sar TaxID=2698737 ... [more]
A0A7S2W799_9STRA2.260e-5661.74Hypothetical protein (Fragment) n=1 Tax=labyrinthu... [more]
A0A812RZ32_SYMMI2.580e-5567.81MhcA protein n=1 Tax=Symbiodinium microadriaticum ... [more]
A0A7S0XE81_9STRA8.730e-5562.58Hypothetical protein (Fragment) n=1 Tax=Chromulina... [more]
A0A7S3PSH7_9STRA1.470e-5456.80Hypothetical protein (Fragment) n=1 Tax=Aplanochyt... [more]
A0A7R9YCN6_9STRA2.410e-5463.87Hypothetical protein (Fragment) n=1 Tax=Pinguiococ... [more]
A0A482S4P7_9ARCH6.600e-5360.26Myosin motor domain-containing protein n=1 Tax=arc... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig93829contigM-pyrifera_M_contig93829:283..747 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score174.5
Seed ortholog evalue3.9e-41
Seed eggNOG ortholog227086.JGI_V11_41654
KEGG koko:K10357,ko:K21868
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005856,GO:0015629,GO:0016459,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017111,GO:0032991,GO:0042623,GO:0043226,GO:0043228,GO:0043229,GO:0043232,GO:0044422,GO:0044424,GO:0044430,GO:0044446,GO:0044464
EggNOG free text desc.translation initiation factor activity
EggNOG OGsCOG5022@1,KOG0160@2759
COG Functional cat.Z
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03019,ko03029,ko04131,ko04812
Exons1
Model size465
Cds size462
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816851.612034-CDS-M-pyrifera_M_contig93829:282..7441622816851.612034-CDS-M-pyrifera_M_contig93829:282..744Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93829 283..744 -
1692278216.0993683-CDS-M-pyrifera_M_contig93829:282..7441692278216.0993683-CDS-M-pyrifera_M_contig93829:282..744Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93829 283..744 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816851.6239586-UTR-M-pyrifera_M_contig93829:744..7471622816851.6239586-UTR-M-pyrifera_M_contig93829:744..747Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig93829 745..747 -
1692278216.110004-UTR-M-pyrifera_M_contig93829:744..7471692278216.110004-UTR-M-pyrifera_M_contig93829:744..747Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig93829 745..747 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig93829.21664.1prot_M-pyrifera_M_contig93829.21664.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig93829 283..744 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig93829.21664.1

>prot_M-pyrifera_M_contig93829.21664.1 ID=prot_M-pyrifera_M_contig93829.21664.1|Name=mRNA_M-pyrifera_M_contig93829.21664.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=154bp
MVKLNDLNEAAILYNLRMRFKADTIYTAVGSILVSVNPFKLLPLYTPQVI
DKYIERGARDLPPHVFGIADFAYRSMLDTSVSQSVVISGESGAGKTEAMK
LILQYLAEVSGRTSGVKPGSVDASESLEQQILKANPVMEAFGNAKTTRNN
NSSR
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mRNA from alignment at M-pyrifera_M_contig93829:283..747-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig93829.21664.1 ID=mRNA_M-pyrifera_M_contig93829.21664.1|Name=mRNA_M-pyrifera_M_contig93829.21664.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=465bp|location=Sequence derived from alignment at M-pyrifera_M_contig93829:283..747- (Macrocystis pyrifera P11B4 male)
AACATGGTGAAGCTGAACGATCTGAACGAGGCCGCAATCCTGTACAACCT GCGCATGCGGTTCAAGGCCGACACCATCTACACCGCTGTCGGCAGTATCC TCGTGTCTGTCAACCCCTTCAAGCTGCTGCCGCTGTACACTCCGCAAGTC ATTGACAAGTACATCGAGCGCGGCGCCCGCGACCTCCCGCCGCACGTGTT CGGCATCGCCGACTTTGCGTACCGCAGCATGCTGGACACCTCCGTTTCCC AGAGCGTGGTCATCAGCGGCGAGTCTGGCGCTGGCAAGACGGAGGCCATG AAGCTCATCCTGCAGTACCTGGCAGAGGTGAGCGGCCGCACGTCCGGCGT CAAGCCGGGCAGCGTGGACGCGTCCGAGTCTCTGGAGCAGCAGATTCTGA AGGCCAACCCTGTGATGGAGGCGTTCGGTAACGCCAAGACCACCCGCAAC AACAACTCGTCCCGC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig93829:283..747-

>mRNA_M-pyrifera_M_contig93829.21664.1 ID=mRNA_M-pyrifera_M_contig93829.21664.1|Name=mRNA_M-pyrifera_M_contig93829.21664.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=924bp|location=Sequence derived from alignment at M-pyrifera_M_contig93829:283..747- (Macrocystis pyrifera P11B4 male)
ATGGTGAAGCTGAACGATCTGAACGAGGCCGCAATCCTGTACAACCTGCG
CATGCGGTTCAAGGCCGACACCATCTACACCGCTGTCGGCAGTATCCTCG
TGTCTGTCAACCCCTTCAAGCTGCTGCCGCTGTACACTCCGCAAGTCATT
GACAAGTACATCGAGCGCGGCGCCCGCGACCTCCCGCCGCACGTGTTCGG
CATCGCCGACTTTGCGTACCGCAGCATGCTGGACACCTCCGTTTCCCAGA
GCGTGGTCATCAGCGGCGAGTCTGGCGCTGGCAAGACGGAGGCCATGAAG
CTCATCCTGCAGTACCTGGCAGAGGTGAGCGGCCGCACGTCCGGCGTCAA
GCCGGGCAGCGTGGACGCGTCCGAGTCTCTGGAGCAGCAGATTCTGAAGG
CCAACCCTGTGATGGAGGCGTTCGGTAACGCCAAGACCACCCGCAACAAC
AACTCGTCCCGCATGGTGAAGCTGAACGATCTGAACGAGGCCGCAATCCT
GTACAACCTGCGCATGCGGTTCAAGGCCGACACCATCTACACCGCTGTCG
GCAGTATCCTCGTGTCTGTCAACCCCTTCAAGCTGCTGCCGCTGTACACT
CCGCAAGTCATTGACAAGTACATCGAGCGCGGCGCCCGCGACCTCCCGCC
GCACGTGTTCGGCATCGCCGACTTTGCGTACCGCAGCATGCTGGACACCT
CCGTTTCCCAGAGCGTGGTCATCAGCGGCGAGTCTGGCGCTGGCAAGACG
GAGGCCATGAAGCTCATCCTGCAGTACCTGGCAGAGGTGAGCGGCCGCAC
GTCCGGCGTCAAGCCGGGCAGCGTGGACGCGTCCGAGTCTCTGGAGCAGC
AGATTCTGAAGGCCAACCCTGTGATGGAGGCGTTCGGTAACGCCAAGACC
ACCCGCAACAACAACTCGTCCCGC
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