mRNA_M-pyrifera_M_contig93758.21655.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig93758.21655.1
Unique NamemRNA_M-pyrifera_M_contig93758.21655.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig93758.21655.1 vs. uniprot
Match: A0A5A8CGT8_CAFRO (FZ domain-containing protein n=3 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CGT8_CAFRO)

HSP 1 Score: 290 bits (741), Expect = 1.410e-96
Identity = 126/184 (68.48%), Postives = 149/184 (80.98%), Query Frame = 1
Query:    4 MPFCRDVVAYRACVPRFQELWPNHTIRAKDEWVQTSHASIVAQRIATERNETLRQSGVNEHGIPGEVEERFADNDDCINAYRNFFCWLNFPRCSADDRSLIMCRSACENYFRACRYDEDMWRCYDPEFYGGKDAEQDEDVDADGLPVFWRAQFPGQPFRENEFDAERNPIEVCTPSLLDAAPPA 555
            MPFC+DVV Y ACVPR+Q L+PNHT+ AKD WV  SHA +VA+R ATERN+TLR  G NEHGIPGEV ERF +N+DCI+AY+NF CWLNFPRC  + RSLI+C S CEN+FR+CRY ED+WRCY+P FYGGK  E+D+DVD DGLPV+WRA FPGQPFR N FD   +PI VCTPS+L+ A  A
Sbjct:   38 MPFCQDVVRYTACVPRYQPLYPNHTLAAKDAWVSASHARVVAERSATERNDTLRSLGENEHGIPGEVVERFFENEDCIDAYKNFLCWLNFPRCDEEGRSLILCGSVCENFFRSCRYSEDLWRCYEPSFYGGKAPEEDKDVDKDGLPVYWRAPFPGQPFRANSFDENGDPIAVCTPSILNGAATA 221          
BLAST of mRNA_M-pyrifera_M_contig93758.21655.1 vs. uniprot
Match: A0A7S1C8M7_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1C8M7_9STRA)

HSP 1 Score: 244 bits (624), Expect = 2.360e-78
Identity = 106/183 (57.92%), Postives = 135/183 (73.77%), Query Frame = 1
Query:    4 MPFCRDVVAYRACVPRFQELWPNHTIRAKDEWVQTSHASIVAQRIATERNETLRQSGVNEHGIPGEVEERFADNDDCINAYRNFFCWLNFPRCSADDRSLIMCRSACENYFRACRYDEDMWRCYDPEFYGGKDAEQDEDVDADGLPVFWRAQFPGQPFRENEFDAERNPIEVCTPSLLDAAPP 552
            MPFC  +V YRACVPRFQEL+ NHT+RAKD+WV  +  +IV  R   E +E L+ +G+ E G  GE+  RF +N DC +AY++F CWLNFPRC  +D S+I+CRS CEN+   C YD D++RC DP FYGG + E D+ +D DGLP++WRA +PGQPFR+NEFD E NPI VCTPS+  AAPP
Sbjct:   78 MPFCAPIVQYRACVPRFQELFSNHTVRAKDDWVARTFKNIVDLRKQYETSEELQDAGITETGEEGEIVVRFFENPDCESAYKHFMCWLNFPRCDEEDASMILCRSVCENFMETCEYDADLYRCGDPRFYGGLEPEADDILDDDGLPIYWRAMYPGQPFRDNEFDEEDNPIAVCTPSIKGAAPP 260          
BLAST of mRNA_M-pyrifera_M_contig93758.21655.1 vs. uniprot
Match: A0A8K1FIR6_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FIR6_PYTOL)

HSP 1 Score: 191 bits (484), Expect = 1.900e-57
Identity = 87/182 (47.80%), Postives = 118/182 (64.84%), Query Frame = 1
Query:    4 MPFCRDVVAYRACVPRFQELWPNHTIRAKDEWVQTSHASIVAQRIATERNETLRQSGVNEHGIPGEVEERFADNDDCINAYRNFFCWLNFPRCSADDRSLIMCRSACENYFRACRYDEDMWRCYDPEFYGGKDAEQDEDVDADGLPVFWRAQFPGQPFRENEFDAERN-PIEVCTPSLLDAA 546
            MPFC+ VV Y AC+PR+Q LW NH+   KD ++      IV QR   E N T      +E G   E+E RF +N DC NA++N+FCWLNFPRC A+ RSL++CRS CENY +AC+  +D+WRC DPE+  G+  EQ      + L V++RA +PG PF++N  D + N P+ +CTPSL + A
Sbjct:   49 MPFCKTVVPYTACLPRYQSLWYNHSALTKDNFISQMFDKIVNQRKMYETNRTFLDKNTDEWGSKNEIEPRFVENPDCENAFKNYFCWLNFPRCDAEGRSLLLCRSVCENYMKACQMSKDLWRCGDPEYVNGRAPEQSVTWVNNEL-VYYRAPYPGSPFKDNAVDPDTNKPLLICTPSLENTA 229          
BLAST of mRNA_M-pyrifera_M_contig93758.21655.1 vs. uniprot
Match: A0A485LR81_9STRA (Aste57867_24742 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485LR81_9STRA)

HSP 1 Score: 190 bits (482), Expect = 2.150e-57
Identity = 86/187 (45.99%), Postives = 122/187 (65.24%), Query Frame = 1
Query:    1 HMPFCRDVVAYRACVPRFQELWPNHTIRAKDEWVQTSHASIVAQRIATERNETLRQSGVNEHGIPGEVEERFADNDDCINAYRNFFCWLNFPRCSADDRSLIMCRSACENYFRACRYDEDMWRCYDPEFYGGKDAEQDEDVDADGLPVFWRAQFPGQPFRENEFDAERNP-IEVCTPSLLDAAPPAT 558
            +MPFC  ++ Y  CVP+ Q LW NH+++ KD ++   +  +V QR   E +  L ++GV+E G  GE+  R+ +N DC NA+RN+ CWLNFPRC     SLIMCRS CEN+F+AC   +D+WRC DP+F  G  AE     +  G+  ++R  FPG PFR+N+F A++   + VCTPSLL+A+P  T
Sbjct:   37 YMPFCGPLLPYSTCVPKAQTLWYNHSVKTKDLFISQMYKKLVTQRELLEADTGLHKAGVDEWGAKGEIVPRYTENVDCQNAFRNYMCWLNFPRCDDAGMSLIMCRSVCENFFKACMQHKDLWRCGDPQFVNGYAAEISTYANQAGVLQYYRYPFPGSPFRDNQFSADKTQALVVCTPSLLNASPRTT 223          
BLAST of mRNA_M-pyrifera_M_contig93758.21655.1 vs. uniprot
Match: A0A5D6YBF1_9STRA (FZ domain-containing protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6YBF1_9STRA)

HSP 1 Score: 191 bits (485), Expect = 4.330e-56
Identity = 87/182 (47.80%), Postives = 123/182 (67.58%), Query Frame = 1
Query:    4 MPFCRDVVAYRACVPRFQELWPNHTIRAKDEWVQTSHASIVAQRIATERNETLRQSGVNEHGIPGEVEERFADNDDCINAYRNFFCWLNFPRCSADDRSLIMCRSACENYFRACRYDEDMWRCYDPEFYGGKDAEQDEDVDADGLPVFWRAQFPGQPFRENEFDAERN-PIEVCTPSLLDAA 546
            +PFC+ VV Y AC+PR+Q LW NH+  +KD++++   + +V QR   E NET      +E G P E+  RF +N DC NA+RN+FCWLNFPRC A+ RSL++CRS CENY +AC+  +D+WRC +P+F  G++ E         L V++RA FPG PF++N  + + N P+ VCTPSL +AA
Sbjct:  179 IPFCKAVVPYTACLPRYQSLWYNHSALSKDKFIEQMFSKLVTQRKQYETNETFLDKNTDEWGGPREIVPRFVENPDCENAFRNYFCWLNFPRCDAEGRSLLLCRSVCENYMKACQLSKDLWRCGEPKFVNGREPETSVTWINKTL-VYYRAPFPGSPFKDNVVNPDTNKPLVVCTPSLENAA 359          
BLAST of mRNA_M-pyrifera_M_contig93758.21655.1 vs. uniprot
Match: A0A024T935_9STRA (FZ domain-containing protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024T935_9STRA)

HSP 1 Score: 187 bits (474), Expect = 5.460e-56
Identity = 85/183 (46.45%), Postives = 122/183 (66.67%), Query Frame = 1
Query:    1 HMPFCRDVVAYRACVPRFQELWPNHTIRAKDEWVQTSHASIVAQRIATERNETLRQSGVNEHGIPGEVEERFADNDDCINAYRNFFCWLNFPRCSADDRSLIMCRSACENYFRACRYDEDMWRCYDPEFYGGKDAEQDEDVDADGLPVFWRAQFPGQPFRENEFDAER-NPIEVCTPSLLDAA 546
            +MPFC  ++ Y  CVPR Q LW NH++++KD ++      ++AQR   E +  + + GV+E G  GE+  R+ +N DC +A+RN+ CWLNFPRC    RSLI+CRS CEN+F+AC   +D+WRC DP++  G  AE     +  G+  ++RA FPG PFR+N F A+R + + VCTPSLL+AA
Sbjct:   48 YMPFCGPLLPYTTCVPRAQTLWYNHSVKSKDLFLAQMFKKMIAQRQIYEADLEMLKQGVDEWGALGEIVPRYTENKDCQDAFRNYMCWLNFPRCDDAGRSLILCRSVCENFFKACMQHKDLWRCGDPQYVNGYSAEISTFANKAGVLQYYRAPFPGSPFRDNAFTADRLDALPVCTPSLLNAA 230          
BLAST of mRNA_M-pyrifera_M_contig93758.21655.1 vs. uniprot
Match: K3WCG6_GLOUD (FZ domain-containing protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WCG6_GLOUD)

HSP 1 Score: 185 bits (470), Expect = 2.190e-55
Identity = 86/188 (45.74%), Postives = 121/188 (64.36%), Query Frame = 1
Query:    4 MPFCRDVVAYRACVPRFQELWPNHTIRAKDEWVQTSHASIVAQRIATERNETLRQSGVNEHGIPGEVEERFADNDDCINAYRNFFCWLNFPRCSADDRSLIMCRSACENYFRACRYDEDMWRCYDPEFYGGKDAEQDEDVDADGLPVFWRAQFPGQPFRENEFDAERN-PIEVCTPSL----LDAAPP 552
            +PFC+ VV Y AC+P++Q LW NH+  +KD++++     I  QR   E N T      +E G   E+  RF DN DC NAYR++FCW+NFPRC A+ RSL++CRS CENY +AC+  +D+WRC DP++  G++AE       + L V++RA FPG PF++N  D++   P+ VCTPSL       APP
Sbjct:   51 IPFCKTVVPYTACLPKYQSLWYNHSALSKDKFIEQMFTKISMQRKMYETNTTFLDKNTDEWGGKKEIVPRFVDNPDCENAYRSYFCWMNFPRCDAEGRSLLLCRSVCENYMKACQLSKDLWRCGDPKYVNGREAETSVTWINNAL-VYYRAPFPGSPFKDNAVDSDTGKPLIVCTPSLENESASIAPP 237          
BLAST of mRNA_M-pyrifera_M_contig93758.21655.1 vs. uniprot
Match: W4FF43_9STRA (FZ domain-containing protein n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=W4FF43_9STRA)

HSP 1 Score: 184 bits (468), Expect = 3.260e-55
Identity = 83/183 (45.36%), Postives = 120/183 (65.57%), Query Frame = 1
Query:    1 HMPFCRDVVAYRACVPRFQELWPNHTIRAKDEWVQTSHASIVAQRIATERNETLRQSGVNEHGIPGEVEERFADNDDCINAYRNFFCWLNFPRCSADDRSLIMCRSACENYFRACRYDEDMWRCYDPEFYGGKDAEQDEDVDADGLPVFWRAQFPGQPFRENEFDAER-NPIEVCTPSLLDAA 546
            +MPFC  ++ Y  CVPR Q LW NH++++KD ++      IV QR   E +  ++ +GV+E G  GE+  R+ +N DC +A+RN+ CWLNFPRC    RSL++CRS CEN+F+AC   +D+WRC  P++  G  AE     +  G+  ++RA FPG PFR+N F A++   + VCTPSLL+AA
Sbjct:   41 YMPFCGPILPYTTCVPRAQTLWYNHSVKSKDLFLAQMFKKIVVQRQLYEADVAMQSAGVDEWGGLGEIVPRYTENKDCQDAFRNYMCWLNFPRCDDAGRSLVLCRSVCENFFKACMQPKDLWRCGQPQYVDGYTAEISTSANMAGVLQYYRAPFPGSPFRDNAFTADKLEALAVCTPSLLNAA 223          
BLAST of mRNA_M-pyrifera_M_contig93758.21655.1 vs. uniprot
Match: A0A2D4CH02_PYTIN (FZ domain-containing protein n=2 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4CH02_PYTIN)

HSP 1 Score: 184 bits (467), Expect = 5.210e-55
Identity = 84/182 (46.15%), Postives = 116/182 (63.74%), Query Frame = 1
Query:    4 MPFCRDVVAYRACVPRFQELWPNHTIRAKDEWVQTSHASIVAQRIATERNETLRQSGVNEHGIPGEVEERFADNDDCINAYRNFFCWLNFPRCSADDRSLIMCRSACENYFRACRYDEDMWRCYDPEFYGGKDAEQDEDVDADGLPVFWRAQFPGQPFRENEFD-AERNPIEVCTPSLLDAA 546
            +PFC+ VV Y AC+PR+Q LW NH+   KD++V+     +V QR   E N T      +E G   E++ RF +N DC NA+RN+FCWLNFPRC A+ RSL++CRS CENY +AC+   D+WRC DP++  G+  E           V++RA +PG PF++N  D A  +P+ VCTPSL + A
Sbjct:   42 LPFCKTVVPYTACLPRYQSLWYNHSALTKDKFVEQMFNKLVTQRKMYETNRTFLDKNTDEWGSKHEIQPRFIENPDCENAFRNYFCWLNFPRCDAEGRSLLLCRSVCENYMKACQMSSDLWRCGDPKYVNGRSPEISSTWINKAF-VYYRAPYPGSPFKDNAVDPATGHPLVVCTPSLENGA 222          
BLAST of mRNA_M-pyrifera_M_contig93758.21655.1 vs. uniprot
Match: A0A0P1AJI9_PLAHL (Frizzled domain n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1AJI9_PLAHL)

HSP 1 Score: 184 bits (468), Expect = 5.250e-55
Identity = 83/182 (45.60%), Postives = 118/182 (64.84%), Query Frame = 1
Query:    4 MPFCRDVVAYRACVPRFQELWPNHTIRAKDEWVQTSHASIVAQRIATERNETLRQSGVNEHGIPGEVEERFADNDDCINAYRNFFCWLNFPRCSADDRSLIMCRSACENYFRACRYDEDMWRCYDPEFYGGKDAEQDEDVDADGLPVFWRAQFPGQPFRENEFDAERN-PIEVCTPSLLDAA 546
            +PFC+ V+ Y AC+PR+Q +W NH+  +KD +V+     IV QR   E N T+     +E G P  +  RF  N DC N+ RN+FCWLNFPRC  + RSL++CRS CEN+  +C+Y +D+WRC DPE+  G+ AE   +   D   V++RA +PG PF++N FD   + P+ VCTPSL ++A
Sbjct:   17 LPFCKAVIPYPACLPRYQSIWYNHSALSKDRFVEQLFHKIVNQRKIDETNVTMADYNTDEWGGPRTITPRFTGNSDCENSLRNYFCWLNFPRCDTEGRSLLLCRSVCENFMTSCQYSKDLWRCGDPEYINGRSAEISTEW-VDQKQVYYRAPYPGSPFKDNAFDPNTDKPLIVCTPSLENSA 197          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig93758.21655.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8CGT8_CAFRO1.410e-9668.48FZ domain-containing protein n=3 Tax=Cafeteria roe... [more]
A0A7S1C8M7_9STRA2.360e-7857.92Hypothetical protein (Fragment) n=1 Tax=Bicosoecid... [more]
A0A8K1FIR6_PYTOL1.900e-5747.80Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A485LR81_9STRA2.150e-5745.99Aste57867_24742 protein n=1 Tax=Aphanomyces stella... [more]
A0A5D6YBF1_9STRA4.330e-5647.80FZ domain-containing protein n=1 Tax=Pythium brass... [more]
A0A024T935_9STRA5.460e-5646.45FZ domain-containing protein n=1 Tax=Aphanomyces i... [more]
K3WCG6_GLOUD2.190e-5545.74FZ domain-containing protein n=1 Tax=Globisporangi... [more]
W4FF43_9STRA3.260e-5545.36FZ domain-containing protein n=1 Tax=Aphanomyces a... [more]
A0A2D4CH02_PYTIN5.210e-5546.15FZ domain-containing protein n=2 Tax=Pythium insid... [more]
A0A0P1AJI9_PLAHL5.250e-5545.60Frizzled domain n=1 Tax=Plasmopara halstedii TaxID... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig93758contigM-pyrifera_M_contig93758:129..746 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score199.5
Seed ortholog evalue1.5e-48
Seed eggNOG ortholog36331.EPrPI00000021293
Hectar predicted targeting categoryother localisation
EggNOG free text desc.function. Source PGD
EggNOG OGs1MBHP@121069,2CB5P@1,2RXQZ@2759
Ec32 ortholog descriptionFrizzled domain
Ec32 orthologEc-17_001260.1
COG Functional cat.T
Best tax levelPythiales
Best eggNOG OGNA|NA|NA
Exons1
Model size618
Cds size615
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816850.943961-CDS-M-pyrifera_M_contig93758:128..7431622816850.943961-CDS-M-pyrifera_M_contig93758:128..743Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93758 129..743 -
1692278215.6276295-CDS-M-pyrifera_M_contig93758:128..7431692278215.6276295-CDS-M-pyrifera_M_contig93758:128..743Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93758 129..743 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816850.9570765-UTR-M-pyrifera_M_contig93758:743..7461622816850.9570765-UTR-M-pyrifera_M_contig93758:743..746Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig93758 744..746 -
1692278215.6431808-UTR-M-pyrifera_M_contig93758:743..7461692278215.6431808-UTR-M-pyrifera_M_contig93758:743..746Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig93758 744..746 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig93758.21655.1prot_M-pyrifera_M_contig93758.21655.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig93758 129..743 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig93758.21655.1

>prot_M-pyrifera_M_contig93758.21655.1 ID=prot_M-pyrifera_M_contig93758.21655.1|Name=mRNA_M-pyrifera_M_contig93758.21655.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=205bp
MPFCRDVVAYRACVPRFQELWPNHTIRAKDEWVQTSHASIVAQRIATERN
ETLRQSGVNEHGIPGEVEERFADNDDCINAYRNFFCWLNFPRCSADDRSL
IMCRSACENYFRACRYDEDMWRCYDPEFYGGKDAEQDEDVDADGLPVFWR
AQFPGQPFRENEFDAERNPIEVCTPSLLDAAPPATTTIALSITLTLAIIA
IVLA*
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mRNA from alignment at M-pyrifera_M_contig93758:129..746-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig93758.21655.1 ID=mRNA_M-pyrifera_M_contig93758.21655.1|Name=mRNA_M-pyrifera_M_contig93758.21655.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=618bp|location=Sequence derived from alignment at M-pyrifera_M_contig93758:129..746- (Macrocystis pyrifera P11B4 male)
CACATGCCGTTCTGCCGCGACGTGGTGGCGTATCGCGCGTGTGTGCCCCG GTTCCAGGAGCTGTGGCCGAACCACACGATCCGCGCCAAGGACGAGTGGG TGCAGACGTCACACGCGTCCATCGTGGCGCAGCGCATAGCGACAGAGCGG AATGAGACGCTGCGACAAAGCGGTGTGAATGAGCACGGAATTCCGGGGGA GGTGGAGGAAAGGTTTGCCGACAACGACGACTGCATCAATGCCTACCGCA ACTTCTTCTGCTGGCTCAACTTCCCGCGCTGCAGTGCAGATGACAGGTCC CTCATCATGTGCAGGTCTGCCTGCGAAAACTACTTTCGTGCCTGCAGATA CGACGAAGACATGTGGCGCTGCTACGATCCAGAATTCTACGGAGGGAAAG ACGCCGAGCAAGACGAAGACGTGGACGCCGATGGACTTCCCGTCTTCTGG AGGGCGCAGTTCCCAGGACAACCGTTCCGGGAAAACGAATTCGACGCAGA ACGAAACCCCATCGAAGTCTGCACCCCTTCGCTCCTAGACGCCGCACCTC CTGCCACAACCACCATCGCTCTCTCCATCACCCTCACCCTCGCCATCATC GCCATCGTGCTCGCATAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig93758:129..746-

>mRNA_M-pyrifera_M_contig93758.21655.1 ID=mRNA_M-pyrifera_M_contig93758.21655.1|Name=mRNA_M-pyrifera_M_contig93758.21655.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1230bp|location=Sequence derived from alignment at M-pyrifera_M_contig93758:129..746- (Macrocystis pyrifera P11B4 male)
ATGCCGTTCTGCCGCGACGTGGTGGCGTATCGCGCGTGTGTGCCCCGGTT
CCAGGAGCTGTGGCCGAACCACACGATCCGCGCCAAGGACGAGTGGGTGC
AGACGTCACACGCGTCCATCGTGGCGCAGCGCATAGCGACAGAGCGGAAT
GAGACGCTGCGACAAAGCGGTGTGAATGAGCACGGAATTCCGGGGGAGGT
GGAGGAAAGGTTTGCCGACAACGACGACTGCATCAATGCCTACCGCAACT
TCTTCTGCTGGCTCAACTTCCCGCGCTGCAGTGCAGATGACAGGTCCCTC
ATCATGTGCAGGTCTGCCTGCGAAAACTACTTTCGTGCCTGCAGATACGA
CGAAGACATGTGGCGCTGCTACGATCCAGAATTCTACGGAGGGAAAGACG
CCGAGCAAGACGAAGACGTGGACGCCGATGGACTTCCCGTCTTCTGGAGG
GCGCAGTTCCCAGGACAACCGTTCCGGGAAAACGAATTCGACGCAGAACG
AAACCCCATCGAAGTCTGCACCCCTTCGCTCCTAGACGCCGCACCTCCTG
CCACAACCACCATCGCTCTCTCCATCACCCTCACCCTCGCCATCATCGCC
ATCGTGCTCGCATAAATGCCGTTCTGCCGCGACGTGGTGGCGTATCGCGC
GTGTGTGCCCCGGTTCCAGGAGCTGTGGCCGAACCACACGATCCGCGCCA
AGGACGAGTGGGTGCAGACGTCACACGCGTCCATCGTGGCGCAGCGCATA
GCGACAGAGCGGAATGAGACGCTGCGACAAAGCGGTGTGAATGAGCACGG
AATTCCGGGGGAGGTGGAGGAAAGGTTTGCCGACAACGACGACTGCATCA
ATGCCTACCGCAACTTCTTCTGCTGGCTCAACTTCCCGCGCTGCAGTGCA
GATGACAGGTCCCTCATCATGTGCAGGTCTGCCTGCGAAAACTACTTTCG
TGCCTGCAGATACGACGAAGACATGTGGCGCTGCTACGATCCAGAATTCT
ACGGAGGGAAAGACGCCGAGCAAGACGAAGACGTGGACGCCGATGGACTT
CCCGTCTTCTGGAGGGCGCAGTTCCCAGGACAACCGTTCCGGGAAAACGA
ATTCGACGCAGAACGAAACCCCATCGAAGTCTGCACCCCTTCGCTCCTAG
ACGCCGCACCTCCTGCCACAACCACCATCGCTCTCTCCATCACCCTCACC
CTCGCCATCATCGCCATCGTGCTCGCATAA
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