mRNA_M-pyrifera_M_contig93414.21575.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig93414.21575.1
Unique NamemRNA_M-pyrifera_M_contig93414.21575.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig93414.21575.1 vs. uniprot
Match: A9UUX2_MONBE (Predicted protein n=1 Tax=Monosiga brevicollis TaxID=81824 RepID=A9UUX2_MONBE)

HSP 1 Score: 177 bits (448), Expect = 6.950e-49
Identity = 96/212 (45.28%), Postives = 124/212 (58.49%), Query Frame = 1
Query:    4 RARAPAPGQFLEWADTLCPNVRV-APCSSTARWGFVYAAGDLLDSYSQPDLTYFKVYHGWTASLHALREIIPTNHTVLLRNPSDRPIGYWPRGDSEGGGRYVVEAIEY-LDEPGEWFFNATSHQV-LYIPEVGESEPQDGDFFASGHSNVLTLAGAHDITFSGLSFRHASWVCNFGGQQVCDSQSTNWQDYATVHVTNGSTRVAVVNSEVSG 630
            RAR P  GQF  W   LCP+VR  A C+  AR+GF YA  D+ +++       F VYHGWTAS H LR I P+   VL  NPSDRPIGYWP  DSEGGGRY VE I   LD   EW+F++T+ ++ L +PE       DGD +     N+L + G H+  FSG+ F + +WVC  G   VCD QS +WQ  A + + +    V   N+E+ G
Sbjct:  145 RARTPNLGQFFVWDRPLCPDVRTNATCAKEARFGFYYAHNDVQEAWRNESNAQFMVYHGWTASRHHLRIIDPSAQRVLFTNPSDRPIGYWPNVDSEGGGRYFVENIRSGLDANFEWYFDSTTRELSLLLPET--LNLTDGDIWIPQTDNLLFVNGTHNHDFSGIDFTYTNWVC--GPTTVCDKQSASWQRQAAIDLFDV-VNVTFSNNEIVG 351          
BLAST of mRNA_M-pyrifera_M_contig93414.21575.1 vs. uniprot
Match: A0A1G0ZG82_9BACT (Uncharacterized protein n=1 Tax=Lentisphaerae bacterium RIFOXYA12_FULL_48_11 TaxID=1798578 RepID=A0A1G0ZG82_9BACT)

HSP 1 Score: 81.6 bits (200), Expect = 2.100e-14
Identity = 58/179 (32.40%), Postives = 83/179 (46.37%), Query Frame = 1
Query:    4 RARAPAPGQFLEWADTLCPNVRVAPCSSTARWGFVYAAGDLLDSYSQPDLTYFKVYHGWTASLHALREIIPTNHTVLLRNPSDR--PIGYWPRGDSEGGGRYVVE-AIEYLDEPGEWFFNATSHQVLYIPEVGESEPQDGDFFASGHSNVLTLAGAHD-------ITFSGLSFRHASWV 510
            RAR P  G F    ++  P  + A     AR  FV+ AGDL       D++   + H W  S+H L+ + P ++ V    P      IGYW +       RY VE A+E LD+PGEW+ N  +  + Y P  GE   +  +  A   + V+  AG  D       +T  GL+F HA W+
Sbjct:  160 RARGPNEGYFR--IESPLPGPKDAHGKEIAREKFVFKAGDLKHWARLNDVS-LTLMHSWETSIHPLKSVDPVSNVVEFAAPMKEWWCIGYWEKNP-----RYYVENALELLDQPGEWYLNRETGVLSYWPMPGEKIGKV-EIVAPFLTEVVRFAGNADEGRFIKHLTLRGLAFHHADWI 329          
BLAST of mRNA_M-pyrifera_M_contig93414.21575.1 vs. uniprot
Match: A0A1V6JNL4_9BACT (Uncharacterized protein n=2 Tax=unclassified Verrucomicrobia TaxID=417295 RepID=A0A1V6JNL4_9BACT)

HSP 1 Score: 78.6 bits (192), Expect = 2.340e-13
Identity = 65/216 (30.09%), Postives = 96/216 (44.44%), Query Frame = 1
Query:    4 RARAPAPGQFLEWADTLCPNVRVAPCSSTARWGFVYAAGDLLDSYSQPDLTYFKVYHGWTASLHALREIIPTNHTVLLRNPSDRPIGYWPRGDSEGGGRYVVE-AIEYLDEPGEWFFNATSHQVLYIPEVGESEPQDGDFFASGHSNVLTLAGAHD-------ITFSGLSFRHASWVCNFGGQQVCDSQSTNWQDYATVHVTNGSTRVAVVNSEVS 627
            RARAP  G +   A  LCP          AR  F +  GD+ + +++ +     + H W  S+H L  +   ++ V L  P +    +W  G  E   RY VE AIE LD+PGEW+ N  S  + Y P  GE   +     A   + ++  AG  D       +   GLSF+H+ WV +  G    +S +    D       NG  R A+ + EV+
Sbjct:  217 RARAPNTGYYRIAA--LCPGPSSPNTPPVARDRFGFRPGDI-EPWARLEDVNIVLMHSWETSIHPLGAVDSVSNLVRLAAPLEE---WWTPGYWEKAQRYYVENAIELLDQPGEWYLNRHSGVLTYWPLPGE-RIETATVVAPELTELVRFAGDPDAGQYVENVILRGLSFQHSDWVLDPKG----NSSTQAAVDVPAAVSANGVRRCAIEDCEVA 421          
BLAST of mRNA_M-pyrifera_M_contig93414.21575.1 vs. uniprot
Match: A0A538NC59_9ACTN (DUF1565 domain-containing protein n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A538NC59_9ACTN)

HSP 1 Score: 75.5 bits (184), Expect = 2.730e-12
Identity = 38/106 (35.85%), Postives = 60/106 (56.60%), Query Frame = 1
Query:  241 NPSDRPIGYWPRGDSEGGGR--YVVEAIEYLDEPGEWFFNATSHQVLYIPEVGESEPQDGDFFASGHSNVLTLAGAHDITFSGLSFRHASWVCNFGGQQVCDSQST 552
            N   R IG    GD   G +  YV  A+E LD+PGEW+ + T+ +V Y+P  GE +P+  D  A     +++    HD+TFSG+ F +A+W+    G+ + + Q+T
Sbjct:  232 NHPTRSIGEVGPGDLHAGRQPAYVENALELLDQPGEWYLDRTAGRVWYLPRAGE-DPRSADIEAPVLETLVSGKSLHDVTFSGIQFAYATWLRPGTGEGLSEVQAT 336          
BLAST of mRNA_M-pyrifera_M_contig93414.21575.1 vs. uniprot
Match: UPI001675938E (Uncharacterized protein n=2 Tax=Promicromonospora soli TaxID=2035533 RepID=UPI001675938E)

HSP 1 Score: 73.9 bits (180), Expect = 9.530e-12
Identity = 53/172 (30.81%), Postives = 77/172 (44.77%), Query Frame = 1
Query:  100 GFVYAAGDLLDSYSQPDLTYFKVYHGWTASLHALREIIPT----NHTVLLRNPSDRPIGYWPRGDSEGGGRYV-VEAIEYLDEPGEWFFNATSHQVLYIPEVGESEPQDGDFFASGHSNVLTLAG------AHDITFSGLSFRHASW-VCNFGGQQVCDSQSTNWQDYATVH 579
            G  Y+  DL    +  D    K    WT ++  +R+I  +    N   L + P        P G  + GG +  + A E+LDEPGE++FN T+H V Y     E       F  +  S +L +AG      A +ITFSGL+  H+ W + N  G      QST     +TV+
Sbjct:  191 GAKYSLSDLPAVAANQDDVEIKSATTWTTAIVGVRQITTSSDGANRVALFQQPGAAIAQAPPYGPFKAGGSHTFMNAYEFLDEPGEFYFNKTTHTVYYYKSGSEDMTTAEVFAPNNVSTLLKIAGTSRTDHARNITFSGLTVEHSDWNLVNVAGSVF--RQSTQGNTISTVY 360          
BLAST of mRNA_M-pyrifera_M_contig93414.21575.1 vs. uniprot
Match: A0A5C1I3X0_9SPHI (Right-handed parallel beta-helix repeat-containing protein n=2 Tax=Mucilaginibacter rubeus TaxID=2027860 RepID=A0A5C1I3X0_9SPHI)

HSP 1 Score: 72.0 bits (175), Expect = 3.920e-11
Identity = 44/150 (29.33%), Postives = 73/150 (48.67%), Query Frame = 1
Query:  133 SYSQPDLTYFK--------VYHGWTASLHALREIIPTNHTVLLRNPSDRPIGYWPRGDSEGGGRYVVE-AIEYLDEPGEWFFNATSHQVLYIPEVGESEPQDGDFFASGHSNVLTLAG-------AHDITFSGLSFRH--ASWVCNFGGQ 528
            SYS  DL   K        +Y+ W AS   + ++      +  RNPS  P+G++     +   RYV+E +++YLD+PGEW+F++ + Q+LY    GE +P    F A     ++   G         +I FS ++F +  ++W  N   Q
Sbjct:  194 SYSGTDLDNLKDIAAADVIIYNSWEASWLRVYKVDKARKVIYFRNPSAYPVGFY-----QPHMRYVIENSLDYLDKPGEWYFDSANKQILYYAATGE-DPNKATFIAPVLDQLVAAKGNAGINRYVSNIKFSNINFSYTKSAWGVNLNQQ 337          
BLAST of mRNA_M-pyrifera_M_contig93414.21575.1 vs. uniprot
Match: A0A519QYB8_FLASP (Right-handed parallel beta-helix repeat-containing protein (Fragment) n=1 Tax=Flavobacterium sp. TaxID=239 RepID=A0A519QYB8_FLASP)

HSP 1 Score: 71.6 bits (174), Expect = 4.030e-11
Identity = 40/141 (28.37%), Postives = 72/141 (51.06%), Query Frame = 1
Query:  100 GFVYAAGDLLDSYSQPDLTYFKVYHGWTASLHALREIIPTNHTVLLRNPSDRPIGYWPRGDSEGGGRYVVE-AIEYLDEPGEWFFNATSHQVLYIPEVGESEPQDGDFFASGHSNVLTLAG-----AHDITFSGLSFRHAS 504
            GF Y   DL D+      T   +YH W  S H +  I  +N T++ +NPS  P+G++         RY+VE +I++L +PG W+ +  + ++ Y   +GE+ P +  F       +++L G      ++I+F  ++F H +
Sbjct:   51 GFTYNNKDL-DNLKYNSGTEINLYHSWETSWHTICNIDTSNKTIIFKNPSTYPVGFFSNHL-----RYIVENSIQFLSKPGRWYLDIENGELYYYANLGEN-PNNMFFIIPKLQELISLKGNPSQLVNNISFFKINFTHTT 184          
BLAST of mRNA_M-pyrifera_M_contig93414.21575.1 vs. uniprot
Match: UPI001AD82DA6 (RICIN domain-containing protein n=1 Tax=Streptomyces capitiformicae TaxID=2014920 RepID=UPI001AD82DA6)

HSP 1 Score: 72.0 bits (175), Expect = 4.360e-11
Identity = 49/164 (29.88%), Postives = 72/164 (43.90%), Query Frame = 1
Query:  100 GFVYAAGDLLDSYSQPDLTYFKVYHGWTASLHALREIIPT----NHTVLLRNPSDRPIGYWPRGDSEGGGRY-VVEAIEYLDEPGEWFFNATSHQVLYIPEVGESEPQDGDFFASGHSNVLTLAG------AHDITFSGLSFRHASW-VCNFGGQQVCDSQSTN 555
            G  Y+  DL    S  D    K    WT ++  +R+I  +    N   L + P        P G+   GG + ++ A E+LD+PGE+FF+ T+H V Y     E       F     S ++ +AG      A +ITFSGL+  H+ W + N  G      Q  N
Sbjct:  193 GAKYSPADLPAVASNQDDVEIKSATTWTTAIVGVRQITTSSDGANRVALFQQPGAAIAQGPPNGNFNPGGTHTLMNAYEFLDQPGEFFFDKTNHLVYYYKSSSEDMTSAQVFAPDNVSTLIKIAGTSTTNHARNITFSGLTVEHSDWNLTNVAGSVFRQGQQGN 356          
BLAST of mRNA_M-pyrifera_M_contig93414.21575.1 vs. uniprot
Match: A0A5Q4GXY8_9PLAN (Right-handed parallel beta-helix repeat-containing protein (Fragment) n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A5Q4GXY8_9PLAN)

HSP 1 Score: 70.1 bits (170), Expect = 1.930e-10
Identity = 52/160 (32.50%), Postives = 75/160 (46.88%), Query Frame = 1
Query:   58 PNVRVAPCSSTARWGFVYAAGDLLDSYSQPDLTYFKVYHGWTASLHALREIIPTNHTVLLRNPSDR--PIGYWPRGDSEGGGRYVVE-AIEYLDEPGEWFFNATSHQVLYIPEVGESEPQDGDFFASGHSNVLTLAGAHD-------ITFSGLSFRHASW 507
            P+ R  P    AR  FV+A GDL   +S+ D     + H W  S+H ++ I    + V    P      IGYW     E   RY VE A+E+L+EPGEW+ + TS  + Y+P  GE   Q  +  A   + ++ L G  +       +   GL+  HA W
Sbjct:  181 PDPRAKPI---ARDKFVFAPGDLRP-WSRLDDVRVVLMHSWETSIHPIKSIDTETNIVRFTAPMSEWWSIGYW-----EPHQRYYVENALEFLNEPGEWYLDRTSGVLSYMPLPGEDLRQV-EVIAPVLTELVRLDGNAEEGRFVDHVVLRGLTLHHADW 330          
BLAST of mRNA_M-pyrifera_M_contig93414.21575.1 vs. uniprot
Match: A0A2G9DL24_9ACTN (Carbohydrate-binding protein n=3 Tax=Streptomyces TaxID=1883 RepID=A0A2G9DL24_9ACTN)

HSP 1 Score: 70.1 bits (170), Expect = 1.990e-10
Identity = 46/157 (29.30%), Postives = 69/157 (43.95%), Query Frame = 1
Query:  100 GFVYAAGDLLDSYSQPDLTYFKVYHGWTASLHALREIIPT----NHTVLLRNPSDRPIGYWPRG--DSEGGGRYVVEAIEYLDEPGEWFFNATSHQVLYIPEVGESEPQDGDFFASGHSNVLTLAG------AHDITFSGLSFRHASWVCNFGGQQV 534
            G  Y+  DL    +  D    +    WT+++  +R+I  +    N   L + P        P G  D+ GG    + A E+LD+PGE++F+ T H V Y     E       F  +  S +L +AG      A +ITFSGL+  H+ W     G  V
Sbjct:  193 GAKYSLADLPAVAANQDDVEIRTQTTWTSAIVGVRQITTSADGANRVALFQQPGAAIAQGPPNGRFDAGGGTHTFMNAYEFLDQPGEFYFDKTQHTVYYYKSGSEDMTSAKVFAPNNVSTLLKIAGTSRTDHARNITFSGLTVEHSDWGLTKVGDSV 349          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig93414.21575.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A9UUX2_MONBE6.950e-4945.28Predicted protein n=1 Tax=Monosiga brevicollis Tax... [more]
A0A1G0ZG82_9BACT2.100e-1432.40Uncharacterized protein n=1 Tax=Lentisphaerae bact... [more]
A0A1V6JNL4_9BACT2.340e-1330.09Uncharacterized protein n=2 Tax=unclassified Verru... [more]
A0A538NC59_9ACTN2.730e-1235.85DUF1565 domain-containing protein n=1 Tax=Actinomy... [more]
UPI001675938E9.530e-1230.81Uncharacterized protein n=2 Tax=Promicromonospora ... [more]
A0A5C1I3X0_9SPHI3.920e-1129.33Right-handed parallel beta-helix repeat-containing... [more]
A0A519QYB8_FLASP4.030e-1128.37Right-handed parallel beta-helix repeat-containing... [more]
UPI001AD82DA64.360e-1129.88RICIN domain-containing protein n=1 Tax=Streptomyc... [more]
A0A5Q4GXY8_9PLAN1.930e-1032.50Right-handed parallel beta-helix repeat-containing... [more]
A0A2G9DL24_9ACTN1.990e-1029.30Carbohydrate-binding protein n=3 Tax=Streptomyces ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig93414contigM-pyrifera_M_contig93414:124..753 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score73.2
Seed ortholog evalue1.7e-10
Seed eggNOG ortholog227086.JGI_V11_71605
Hectar predicted targeting categoryother localisation
EggNOG OGs2E3KA@1,2SAMW@2759
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons1
Model size630
Cds size630
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816846.6174877-CDS-M-pyrifera_M_contig93414:123..7531622816846.6174877-CDS-M-pyrifera_M_contig93414:123..753Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93414 124..753 +
1692278212.9737647-CDS-M-pyrifera_M_contig93414:123..7531692278212.9737647-CDS-M-pyrifera_M_contig93414:123..753Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93414 124..753 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig93414.21575.1prot_M-pyrifera_M_contig93414.21575.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig93414 124..753 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig93414.21575.1

>prot_M-pyrifera_M_contig93414.21575.1 ID=prot_M-pyrifera_M_contig93414.21575.1|Name=mRNA_M-pyrifera_M_contig93414.21575.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=210bp
MRARAPAPGQFLEWADTLCPNVRVAPCSSTARWGFVYAAGDLLDSYSQPD
LTYFKVYHGWTASLHALREIIPTNHTVLLRNPSDRPIGYWPRGDSEGGGR
YVVEAIEYLDEPGEWFFNATSHQVLYIPEVGESEPQDGDFFASGHSNVLT
LAGAHDITFSGLSFRHASWVCNFGGQQVCDSQSTNWQDYATVHVTNGSTR
VAVVNSEVSG
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mRNA from alignment at M-pyrifera_M_contig93414:124..753+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig93414.21575.1 ID=mRNA_M-pyrifera_M_contig93414.21575.1|Name=mRNA_M-pyrifera_M_contig93414.21575.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=630bp|location=Sequence derived from alignment at M-pyrifera_M_contig93414:124..753+ (Macrocystis pyrifera P11B4 male)
ATGCGTGCTCGTGCACCTGCACCTGGTCAATTTTTGGAATGGGCAGATAC ATTGTGTCCCAATGTCCGTGTTGCTCCATGTTCATCCACCGCAAGATGGG GCTTTGTGTATGCTGCTGGGGACTTGCTTGACTCCTACAGTCAGCCAGAT TTGACGTACTTCAAAGTGTATCATGGTTGGACTGCATCACTTCATGCATT GCGTGAGATTATCCCGACCAATCACACCGTTCTCCTCCGAAACCCTTCAG ATCGCCCTATCGGATATTGGCCACGTGGGGATTCAGAAGGCGGTGGAAGG TATGTGGTTGAAGCGATTGAGTACCTTGATGAGCCAGGAGAATGGTTTTT CAATGCAACATCACACCAAGTGCTTTATATACCTGAAGTTGGGGAAAGTG AGCCACAAGATGGTGATTTCTTTGCGTCTGGTCATTCCAATGTGTTGACG TTGGCGGGTGCACATGACATCACATTCAGTGGACTCTCCTTCCGTCATGC ATCGTGGGTGTGCAACTTTGGAGGACAACAAGTTTGTGATAGTCAAAGTA CAAATTGGCAAGACTACGCAACGGTACATGTCACAAACGGATCCACACGT GTGGCAGTCGTGAATTCTGAAGTCTCTGGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig93414:124..753+

>mRNA_M-pyrifera_M_contig93414.21575.1 ID=mRNA_M-pyrifera_M_contig93414.21575.1|Name=mRNA_M-pyrifera_M_contig93414.21575.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1260bp|location=Sequence derived from alignment at M-pyrifera_M_contig93414:124..753+ (Macrocystis pyrifera P11B4 male)
ATGCGTGCTCGTGCACCTGCACCTGGTCAATTTTTGGAATGGGCAGATAC
ATTGTGTCCCAATGTCCGTGTTGCTCCATGTTCATCCACCGCAAGATGGG
GCTTTGTGTATGCTGCTGGGGACTTGCTTGACTCCTACAGTCAGCCAGAT
TTGACGTACTTCAAAGTGTATCATGGTTGGACTGCATCACTTCATGCATT
GCGTGAGATTATCCCGACCAATCACACCGTTCTCCTCCGAAACCCTTCAG
ATCGCCCTATCGGATATTGGCCACGTGGGGATTCAGAAGGCGGTGGAAGG
TATGTGGTTGAAGCGATTGAGTACCTTGATGAGCCAGGAGAATGGTTTTT
CAATGCAACATCACACCAAGTGCTTTATATACCTGAAGTTGGGGAAAGTG
AGCCACAAGATGGTGATTTCTTTGCGTCTGGTCATTCCAATGTGTTGACG
TTGGCGGGTGCACATGACATCACATTCAGTGGACTCTCCTTCCGTCATGC
ATCGTGGGTGTGCAACTTTGGAGGACAACAAGTTTGTGATAGTCAAAGTA
CAAATTGGCAAGACTACGCAACGGTACATGTCACAAACGGATCCACACGT
GTGGCAGTCGTGAATTCTGAAGTCTCTGGAATGCGTGCTCGTGCACCTGC
ACCTGGTCAATTTTTGGAATGGGCAGATACATTGTGTCCCAATGTCCGTG
TTGCTCCATGTTCATCCACCGCAAGATGGGGCTTTGTGTATGCTGCTGGG
GACTTGCTTGACTCCTACAGTCAGCCAGATTTGACGTACTTCAAAGTGTA
TCATGGTTGGACTGCATCACTTCATGCATTGCGTGAGATTATCCCGACCA
ATCACACCGTTCTCCTCCGAAACCCTTCAGATCGCCCTATCGGATATTGG
CCACGTGGGGATTCAGAAGGCGGTGGAAGGTATGTGGTTGAAGCGATTGA
GTACCTTGATGAGCCAGGAGAATGGTTTTTCAATGCAACATCACACCAAG
TGCTTTATATACCTGAAGTTGGGGAAAGTGAGCCACAAGATGGTGATTTC
TTTGCGTCTGGTCATTCCAATGTGTTGACGTTGGCGGGTGCACATGACAT
CACATTCAGTGGACTCTCCTTCCGTCATGCATCGTGGGTGTGCAACTTTG
GAGGACAACAAGTTTGTGATAGTCAAAGTACAAATTGGCAAGACTACGCA
ACGGTACATGTCACAAACGGATCCACACGTGTGGCAGTCGTGAATTCTGA
AGTCTCTGGA
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