mRNA_M-pyrifera_M_contig93399.21565.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig93399.21565.1
Unique NamemRNA_M-pyrifera_M_contig93399.21565.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig93399.21565.1 vs. uniprot
Match: A0A5A8CC32_CAFRO (DAO domain-containing protein n=2 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CC32_CAFRO)

HSP 1 Score: 164 bits (415), Expect = 1.310e-46
Identity = 84/132 (63.64%), Postives = 100/132 (75.76%), Query Frame = 1
Query:    1 TAIPSLWREQYGFT-NPNLEELVETLSTEANLFLFGANFPFRELAWKEARKYSKSFMAQGASELAHGVDLSKFTTYGKPGIRAQLLDLKKRALVMDFLVEGDQGSTHVLNAVSPAWTCSQPFAEHVVKNIKV 393
            TAIP+LWREQYG   N ++ ++  TL TEA LF+  A F FRELA++EARKY K FM +GASEL  GV  + F  YG+PGIRAQLLD + R LVMDF +EGD+ STHVLNAVSPAWTCS PFA+HV   +KV
Sbjct:  279 TAIPALWREQYGLLDNMDIGDMYTTLMTEAQLFV-KAGFNFRELAFEEARKYYKPFMTKGASELVKGVTDASFGNYGRPGIRAQLLDKRTRTLVMDFHIEGDESSTHVLNAVSPAWTCSVPFAQHVCDTMKV 409          
BLAST of mRNA_M-pyrifera_M_contig93399.21565.1 vs. uniprot
Match: A0A812KYD7_9DINO (Hypothetical protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A812KYD7_9DINO)

HSP 1 Score: 164 bits (415), Expect = 3.880e-46
Identity = 84/132 (63.64%), Postives = 100/132 (75.76%), Query Frame = 1
Query:    1 TAIPSLWREQYGFT-NPNLEELVETLSTEANLFLFGANFPFRELAWKEARKYSKSFMAQGASELAHGVDLSKFTTYGKPGIRAQLLDLKKRALVMDFLVEGDQGSTHVLNAVSPAWTCSQPFAEHVVKNIKV 393
            TAIP+LWREQYG   N ++ ++  TL TEA LF+  A F FRELA++EARKY K FM +GASEL  GV  + F  YG+PGIRAQLLD + R LVMDF +EGD+ STHVLNAVSPAWTCS PFA+HV   +KV
Sbjct:  334 TAIPALWREQYGLLDNMDIGDMYTTLMTEAQLFV-KAGFNFRELAFEEARKYYKPFMTKGASELVKGVTDASFGNYGRPGIRAQLLDKRTRTLVMDFHIEGDESSTHVLNAVSPAWTCSVPFAQHVCDTMKV 464          
BLAST of mRNA_M-pyrifera_M_contig93399.21565.1 vs. uniprot
Match: A0A7W1TFR7_9BACT (L-2-hydroxyglutarate oxidase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A7W1TFR7_9BACT)

HSP 1 Score: 149 bits (375), Expect = 9.090e-41
Identity = 79/130 (60.77%), Postives = 90/130 (69.23%), Query Frame = 1
Query:    1 TAIPSLWREQY-GFTNPNLEELVETLSTEANLFLFGANFPFRELAWKEARKYSKSFMAQGASELAHGVDLSKFTTYGKPGIRAQLLDLKKRALVMDFLVEGDQGSTHVLNAVSPAWTCSQPFAEHVVKNI 387
            TAIP+ WRE Y GF   +L EL E L  EA L L  A F FR LA +E RKY  SF+   A+ LA GVD  ++T +G+PGIRAQLLDLK R LVMDF +EGD  S HVLNAVSPAWTCS PFA HVV  +
Sbjct:  266 TAIPAFWREHYQGFERFSLSELGEVLWREATLML-SAGFDFRGLAVQELRKYDPSFLVAQATALAEGVDQERYTRWGRPGIRAQLLDLKTRTLVMDFCIEGDAQSLHVLNAVSPAWTCSLPFAAHVVDEV 394          
BLAST of mRNA_M-pyrifera_M_contig93399.21565.1 vs. uniprot
Match: A0A2V5W502_9BACT (L-2-hydroxyglutarate oxidase n=1 Tax=Verrucomicrobia bacterium TaxID=2026799 RepID=A0A2V5W502_9BACT)

HSP 1 Score: 146 bits (368), Expect = 1.160e-39
Identity = 75/131 (57.25%), Postives = 90/131 (68.70%), Query Frame = 1
Query:    1 TAIPSLWREQY-GFTNPNLEELVETLSTEANLFLFGANFPFRELAWKEARKYSKSFMAQGASELAHGVDLSKFTTYGKPGIRAQLLDLKKRALVMDFLVEGDQGSTHVLNAVSPAWTCSQPFAEHVVKNIK 390
            TAIP+ WREQY GF N    E VE L  EA L +  + F F++LA +E RKYS+  +   AS LAHGV    +  +G+PGIRAQLLD+K R L MDF +EGD+ STH+LNAVSP WTCS PFA HVV  IK
Sbjct:  276 TAIPAFWREQYRGFDNFKFGEFVEVLFREAGLMI-SSTFDFKQLALEEIRKYSRPRLVALASRLAHGVQPEHYRRWGEPGIRAQLLDVKSRKLEMDFAIEGDEHSTHLLNAVSPGWTCSMPFASHVVDRIK 405          
BLAST of mRNA_M-pyrifera_M_contig93399.21565.1 vs. uniprot
Match: A0A845K0P1_9BACT (Uncharacterized protein n=1 Tax=Nitrospinae bacterium TaxID=2026769 RepID=A0A845K0P1_9BACT)

HSP 1 Score: 138 bits (348), Expect = 1.410e-39
Identity = 72/130 (55.38%), Postives = 90/130 (69.23%), Query Frame = 1
Query:    1 TAIPSLWREQY-GFTNPNLEELVETLSTEANLFLFGANFPFRELAWKEARKYSKSFMAQGASELAHGVDLSKFTTYGKPGIRAQLLDLKKRALVMDFLVEGDQGSTHVLNAVSPAWTCSQPFAEHVVKNI 387
            TAIP+ WREQY G  N  L E  E    +A LF F ++F F++LAW+E RKYS++ + + AS L   V  S +  +GKPGIRAQLL++KK  L MDF+VEGD  S HVLNAVSPA+TCS PFAE+V   I
Sbjct:    7 TAIPAFWREQYTGLNNFRLNEFTELTLRQALLF-FNSSFDFKQLAWQEIRKYSQTHLVKLASALLENVKASNYKKWGKPGIRAQLLNIKKNTLEMDFIVEGDHQSIHVLNAVSPAFTCSFPFAEYVCDKI 135          
BLAST of mRNA_M-pyrifera_M_contig93399.21565.1 vs. uniprot
Match: A0A2H0MHN3_9BACT (L-2-hydroxyglutarate oxidase n=1 Tax=Nitrospinae bacterium CG11_big_fil_rev_8_21_14_0_20_56_8 TaxID=1974049 RepID=A0A2H0MHN3_9BACT)

HSP 1 Score: 143 bits (361), Expect = 1.510e-38
Identity = 74/130 (56.92%), Postives = 97/130 (74.62%), Query Frame = 1
Query:    1 TAIPSLWREQY-GFTNPNLEELVETLSTEANLFLFGANFPFRELAWKEARKYSKSFMAQGASELAHGVDLSKFTTYGKPGIRAQLLDLKKRALVMDFLVEGDQGSTHVLNAVSPAWTCSQPFAEHVVKNI 387
            TAIP+ WREQY GF N +  EL+E L  +A LFL  +NF F++LA++E RKYS++++   ASELA G++ + FT +G+PGIRAQL DLK + L MDF++EGD  S HVLNAVSPA+TC+ PFAE V   I
Sbjct:  287 TAIPAFWREQYEGFENFDWGELIEILFRQAGLFL-KSNFDFKKLAYEEIRKYSRNYLVNLASELAEGINPNHFTQWGRPGIRAQLFDLKTKKLEMDFVLEGDAQSLHVLNAVSPAFTCAFPFAEFVCDRI 415          
BLAST of mRNA_M-pyrifera_M_contig93399.21565.1 vs. uniprot
Match: A0A2W5YVB5_9BACT (L-2-hydroxyglutarate oxidase n=1 Tax=Chthoniobacterales bacterium TaxID=2201154 RepID=A0A2W5YVB5_9BACT)

HSP 1 Score: 142 bits (359), Expect = 2.040e-38
Identity = 72/131 (54.96%), Postives = 93/131 (70.99%), Query Frame = 1
Query:    1 TAIPSLWREQYG-FTNPNLEELVETLSTEANLFLFGANFPFRELAWKEARKYSKSFMAQGASELAHGVDLSKFTTYGKPGIRAQLLDLKKRALVMDFLVEGDQGSTHVLNAVSPAWTCSQPFAEHVVKNIK 390
            TAIP+ WREQYG F N    E +E L  EA L +F ++F F++LA +E +KYS+  +   AS+LA GVD   +  +G PGIRAQLLD+K R L MDF++EGD+ STH+LNAVSP WTCS PFA +VV  I+
Sbjct:  266 TAIPAFWREQYGAFDNFRFGEFIEILFREAGL-MFSSSFDFKQLAVEELKKYSRPHLVALASQLATGVDTRHYRRWGPPGIRAQLLDIKSRKLEMDFVIEGDERSTHLLNAVSPGWTCSLPFASYVVDRIE 395          
BLAST of mRNA_M-pyrifera_M_contig93399.21565.1 vs. uniprot
Match: A0A7W1FPJ5_9BACT (L-2-hydroxyglutarate oxidase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A7W1FPJ5_9BACT)

HSP 1 Score: 142 bits (358), Expect = 2.860e-38
Identity = 73/127 (57.48%), Postives = 93/127 (73.23%), Query Frame = 1
Query:    1 TAIPSLWREQY-GFTNPNLEELVETLSTEANLFLFGANFPFRELAWKEARKYSKSFMAQGASELAHGVDLSKFTTYGKPGIRAQLLDLKKRALVMDFLVEGDQGSTHVLNAVSPAWTCSQPFAEHVV 378
            TAIP+ WRE Y G  + +L E++E    EA+L L  A F FR LA +E +K+S SF+   A++L  GV L  +T +G+PGIRAQLLDL+KR LVMDF+VEG++ S HVLNAVSPAWTC+ PFA HVV
Sbjct:  265 TAIPAFWREHYSGLEHFDLAEMIEITLREASLMLH-AGFDFRGLALQEMKKFSPSFLVGQAAQLLEGVHLEDYTRWGRPGIRAQLLDLEKRTLVMDFVVEGNRQSLHVLNAVSPAWTCAMPFAAHVV 390          
BLAST of mRNA_M-pyrifera_M_contig93399.21565.1 vs. uniprot
Match: A0A2V5PWG4_9BACT (L-2-hydroxyglutarate oxidase n=1 Tax=Verrucomicrobia bacterium TaxID=2026799 RepID=A0A2V5PWG4_9BACT)

HSP 1 Score: 141 bits (356), Expect = 6.170e-38
Identity = 72/131 (54.96%), Postives = 93/131 (70.99%), Query Frame = 1
Query:    1 TAIPSLWREQY-GFTNPNLEELVETLSTEANLFLFGANFPFRELAWKEARKYSKSFMAQGASELAHGVDLSKFTTYGKPGIRAQLLDLKKRALVMDFLVEGDQGSTHVLNAVSPAWTCSQPFAEHVVKNIK 390
            TAIP+LWREQY GF N    E +E L  EA L +  + F F++LA +E +KYS+  +   AS LAHGV   ++T +G+PGIRAQLLD+K R L MDF++EGD+ STH+LNAVSP WTCS PFA + V  I+
Sbjct:  271 TAIPALWREQYRGFDNFKFGEFIEVLFREAGLMM-SSTFDFQKLAVEELQKYSRPRLVALASRLAHGVRPEQYTRWGQPGIRAQLLDIKNRKLEMDFVIEGDEHSTHLLNAVSPGWTCSIPFARYAVDQIE 400          
BLAST of mRNA_M-pyrifera_M_contig93399.21565.1 vs. uniprot
Match: A0A2E6URN0_9PROT (L-2-hydroxyglutarate oxidase n=1 Tax=Nitrosomonadaceae bacterium TaxID=2026767 RepID=A0A2E6URN0_9PROT)

HSP 1 Score: 140 bits (353), Expect = 1.560e-37
Identity = 69/131 (52.67%), Postives = 92/131 (70.23%), Query Frame = 1
Query:    1 TAIPSLWREQY-GFTNPNLEELVETLSTEANLFLFGANFPFRELAWKEARKYSKSFMAQGASELAHGVDLSKFTTYGKPGIRAQLLDLKKRALVMDFLVEGDQGSTHVLNAVSPAWTCSQPFAEHVVKNIK 390
            TAIP+ WREQY G  N +L E +E    +A LF F ++F F++LAW+E RKYS++ +   AS L   V +S F  +GKPGIRAQLL++K   L MDF++EGD  S HVLNAVSPA+TC+ PFA+H+   I+
Sbjct:  266 TAIPAFWREQYTGINNFHLNEFLELSLCQAKLF-FNSSFDFKQLAWREIRKYSRAHLVNLASTLLQDVKISNFKDWGKPGIRAQLLNIKNNTLEMDFIIEGDHQSIHVLNAVSPAFTCAFPFADHICNKIE 395          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig93399.21565.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8CC32_CAFRO1.310e-4663.64DAO domain-containing protein n=2 Tax=Cafeteria ro... [more]
A0A812KYD7_9DINO3.880e-4663.64Hypothetical protein n=1 Tax=Symbiodinium sp. KB8 ... [more]
A0A7W1TFR7_9BACT9.090e-4160.77L-2-hydroxyglutarate oxidase n=1 Tax=Planctomycete... [more]
A0A2V5W502_9BACT1.160e-3957.25L-2-hydroxyglutarate oxidase n=1 Tax=Verrucomicrob... [more]
A0A845K0P1_9BACT1.410e-3955.38Uncharacterized protein n=1 Tax=Nitrospinae bacter... [more]
A0A2H0MHN3_9BACT1.510e-3856.92L-2-hydroxyglutarate oxidase n=1 Tax=Nitrospinae b... [more]
A0A2W5YVB5_9BACT2.040e-3854.96L-2-hydroxyglutarate oxidase n=1 Tax=Chthoniobacte... [more]
A0A7W1FPJ5_9BACT2.860e-3857.48L-2-hydroxyglutarate oxidase n=1 Tax=Planctomycete... [more]
A0A2V5PWG4_9BACT6.170e-3854.96L-2-hydroxyglutarate oxidase n=1 Tax=Verrucomicrob... [more]
A0A2E6URN0_9PROT1.560e-3752.67L-2-hydroxyglutarate oxidase n=1 Tax=Nitrosomonada... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig93399contigM-pyrifera_M_contig93399:2..397 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score134.0
Seed ortholog evalue5e-29
Seed eggNOG ortholog1131269.AQVV01000027_gene90
Preferred namelhgO
KEGG rclassRC00031
KEGG koko:K00109,ko:K15736
KEGG ReactionR03534
KEGG Pathwayko00650,map00650
Hectar predicted targeting categoryother localisation
EggNOG free text desc.malate dehydrogenase (menaquinone) activity
EggNOG OGsCOG0579@1,COG0579@2
EC1.1.99.2
COG Functional cat.S
Best tax levelBacteria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons1
Model size396
Cds size396
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816845.949712-CDS-M-pyrifera_M_contig93399:1..3971622816845.949712-CDS-M-pyrifera_M_contig93399:1..397Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93399 2..397 +
1692278212.5959086-CDS-M-pyrifera_M_contig93399:1..3971692278212.5959086-CDS-M-pyrifera_M_contig93399:1..397Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93399 2..397 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig93399.21565.1prot_M-pyrifera_M_contig93399.21565.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig93399 2..397 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig93399.21565.1

>prot_M-pyrifera_M_contig93399.21565.1 ID=prot_M-pyrifera_M_contig93399.21565.1|Name=mRNA_M-pyrifera_M_contig93399.21565.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=132bp
TAIPSLWREQYGFTNPNLEELVETLSTEANLFLFGANFPFRELAWKEARK
YSKSFMAQGASELAHGVDLSKFTTYGKPGIRAQLLDLKKRALVMDFLVEG
DQGSTHVLNAVSPAWTCSQPFAEHVVKNIKV*
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mRNA from alignment at M-pyrifera_M_contig93399:2..397+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig93399.21565.1 ID=mRNA_M-pyrifera_M_contig93399.21565.1|Name=mRNA_M-pyrifera_M_contig93399.21565.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=396bp|location=Sequence derived from alignment at M-pyrifera_M_contig93399:2..397+ (Macrocystis pyrifera P11B4 male)
ACTGCTATTCCCTCCCTGTGGAGAGAACAGTATGGTTTCACCAACCCCAA CCTTGAAGAGCTTGTAGAAACACTCTCCACCGAAGCCAACCTCTTCCTCT TTGGTGCAAACTTCCCTTTCCGAGAACTTGCATGGAAAGAAGCCAGAAAG TACTCCAAGTCATTCATGGCACAGGGTGCCTCTGAGCTCGCACACGGTGT AGACCTTTCCAAGTTCACAACCTATGGAAAACCTGGCATTCGAGCTCAAC TTCTCGACCTCAAAAAGCGAGCACTTGTGATGGACTTCCTCGTCGAAGGT GATCAAGGATCCACCCACGTCCTCAACGCAGTCTCCCCAGCATGGACATG CTCCCAGCCTTTTGCAGAACACGTTGTTAAGAACATCAAAGTTTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig93399:2..397+

>mRNA_M-pyrifera_M_contig93399.21565.1 ID=mRNA_M-pyrifera_M_contig93399.21565.1|Name=mRNA_M-pyrifera_M_contig93399.21565.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=792bp|location=Sequence derived from alignment at M-pyrifera_M_contig93399:2..397+ (Macrocystis pyrifera P11B4 male)
ACTGCTATTCCCTCCCTGTGGAGAGAACAGTATGGTTTCACCAACCCCAA
CCTTGAAGAGCTTGTAGAAACACTCTCCACCGAAGCCAACCTCTTCCTCT
TTGGTGCAAACTTCCCTTTCCGAGAACTTGCATGGAAAGAAGCCAGAAAG
TACTCCAAGTCATTCATGGCACAGGGTGCCTCTGAGCTCGCACACGGTGT
AGACCTTTCCAAGTTCACAACCTATGGAAAACCTGGCATTCGAGCTCAAC
TTCTCGACCTCAAAAAGCGAGCACTTGTGATGGACTTCCTCGTCGAAGGT
GATCAAGGATCCACCCACGTCCTCAACGCAGTCTCCCCAGCATGGACATG
CTCCCAGCCTTTTGCAGAACACGTTGTTAAGAACATCAAAGTTTGAACTG
CTATTCCCTCCCTGTGGAGAGAACAGTATGGTTTCACCAACCCCAACCTT
GAAGAGCTTGTAGAAACACTCTCCACCGAAGCCAACCTCTTCCTCTTTGG
TGCAAACTTCCCTTTCCGAGAACTTGCATGGAAAGAAGCCAGAAAGTACT
CCAAGTCATTCATGGCACAGGGTGCCTCTGAGCTCGCACACGGTGTAGAC
CTTTCCAAGTTCACAACCTATGGAAAACCTGGCATTCGAGCTCAACTTCT
CGACCTCAAAAAGCGAGCACTTGTGATGGACTTCCTCGTCGAAGGTGATC
AAGGATCCACCCACGTCCTCAACGCAGTCTCCCCAGCATGGACATGCTCC
CAGCCTTTTGCAGAACACGTTGTTAAGAACATCAAAGTTTGA
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