mRNA_M-pyrifera_M_contig93308.21548.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig93308.21548.1
Unique NamemRNA_M-pyrifera_M_contig93308.21548.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig93308.21548.1 vs. uniprot
Match: W4FG40_9STRA (Methyltransf_25 domain-containing protein n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=W4FG40_9STRA)

HSP 1 Score: 103 bits (257), Expect = 1.710e-24
Identity = 57/162 (35.19%), Postives = 85/162 (52.47%), Query Frame = 1
Query:    1 VTPKHGSALYVSKEYWEYRFEREGEKEWLADPETVLGLLRGWIGHVEGVGRDVLVVGCGNSALSSALSREEWVKEVLSTDYSASVIAAMKSKYGAEEGRLQWAVADMSELSTQFKSRKFSSILDKGALDAVVADGGDSWSPPDATLARGLQCVEEMSKVLQE 486
            V PK+ +  Y  KEYW+ RFE E   +WLA  + V  L+  ++         +LVVGCGNS  S  L  E     +++ D+S+ VI  M +KY      + W V DM +L+  F    F  ++DK A+DA++ +GGD+WSP D  LA        +  +L +
Sbjct:    2 VLPKNNNE-YKKKEYWDSRFESEESYDWLARFKDVASLIDQYVQK----NHRILVVGCGNSTFSIDLY-EAGYTNIINLDFSSVVIERMSAKYSTSHPLMTWVVGDMLKLNESFPPHSFDVVIDKAAMDALMVEGGDTWSPADHVLANADAMCSGVHSILTQ 157          
BLAST of mRNA_M-pyrifera_M_contig93308.21548.1 vs. uniprot
Match: A0A024UL85_9STRA (Methyltransf_25 domain-containing protein n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024UL85_9STRA)

HSP 1 Score: 103 bits (256), Expect = 2.420e-24
Identity = 56/151 (37.09%), Postives = 81/151 (53.64%), Query Frame = 1
Query:   28 YVSKEYWEYRFEREGEKEWLADPETVLGLLRGWIGHVEGVGRDVLVVGCGNSALSSALSREEWVKEVLSTDYSASVIAAMKSKYGAEEGRLQWAVADMSELSTQFKSRKFSSILDKGALDAVVADGGDSWSPPDATLARGLQCVEEMSKVL 480
            Y  +EYW+ RFE E   +WLA    V  LL  ++       + +L+VGCGNS  S  L  E     +++ D+S  VI  M++KY      + W V DM  L+  F  + F  ++DK A+DA++ +GGD+WSPP   LA G Q    +  VL
Sbjct:   10 YGKQEYWDSRFESEESYDWLARYNDVATLLDQYVR----TDQRILIVGCGNSTFSVDLY-EAGYTNIVNLDFSTVVIDRMRAKYSVSHPLMTWIVGDMLTLTESFAPQSFDVVIDKAAMDALMVEGGDTWSPPSHLLAAGHQMCAGVQSVL 155          
BLAST of mRNA_M-pyrifera_M_contig93308.21548.1 vs. uniprot
Match: A0A485KM56_9STRA (Aste57867_9023 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KM56_9STRA)

HSP 1 Score: 100 bits (250), Expect = 1.760e-23
Identity = 54/151 (35.76%), Postives = 81/151 (53.64%), Query Frame = 1
Query:   28 YVSKEYWEYRFEREGEKEWLADPETVLGLLRGWIGHVEGVGRDVLVVGCGNSALSSALSREEWVKEVLSTDYSASVIAAMKSKYGAEEGRLQWAVADMSELSTQFKSRKFSSILDKGALDAVVADGGDSWSPPDATLARGLQCVEEMSKVL 480
            Y  +EYW  RFE E   +WLA  + V  L+  ++   +     +L+VGCGNS  S  L  E     + + D+S+ VI  M +KY A    + W V DM +L  QF    F  ++DK A+DA++ +GGD+WSP +  LA G +    +  +L
Sbjct:   12 YKKQEYWNSRFENEETYDWLARYKDVAELIAQYVQKDQ----RLLIVGCGNSTFSIDLY-EAGYTNITNLDFSSVVIERMHAKYSASHPLMTWVVGDMLKLPAQFSPASFDVVIDKAAMDALMVEGGDTWSPAEHLLASGHEMCAGIQSIL 157          
BLAST of mRNA_M-pyrifera_M_contig93308.21548.1 vs. uniprot
Match: A0A1W0A6C7_9STRA (Methyltransf_11 domain-containing protein n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1W0A6C7_9STRA)

HSP 1 Score: 100 bits (249), Expect = 2.420e-23
Identity = 55/158 (34.81%), Postives = 86/158 (54.43%), Query Frame = 1
Query:    7 PKHGSALYVSKEYWEYRFEREGEKEWLADPETVLGLLRGWIGHVEGVGRDVLVVGCGNSALSSALSREEWVKEVLSTDYSASVIAAMKSKYGAEEGRLQWAVADMSELSTQFKSRKFSSILDKGALDAVVADGGDSWSPPDATLARGLQCVEEMSKVL 480
            P+H S  Y ++ YW+ RF  E   +WLA    V  L+  ++         +L+VGCGNS  S  L    +   +++ D+S+ VI  M +KY      ++WAV DM +L   F+S  F  ++DK A+DA++ +GGD+WSP +  LA   +    + KVL
Sbjct:    4 PRHNSE-YKNQSYWDTRFASEESYDWLAKYSDVADLITKYVPKDS----RLLIVGCGNSTFSIDLYDAGYTN-IVNLDFSSVVIERMSAKYSESRRLMKWAVGDMLKLPEFFESNSFDVVIDKAAMDALMVEGGDTWSPAEHLLASAHEMCSGIKKVL 155          
BLAST of mRNA_M-pyrifera_M_contig93308.21548.1 vs. uniprot
Match: A0A6G0XDR0_9STRA (Methyltransf_25 domain-containing protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0XDR0_9STRA)

HSP 1 Score: 99.8 bits (247), Expect = 5.770e-23
Identity = 54/151 (35.76%), Postives = 80/151 (52.98%), Query Frame = 1
Query:   28 YVSKEYWEYRFEREGEKEWLADPETVLGLLRGWIGHVEGVGRDVLVVGCGNSALSSALSREEWVKEVLSTDYSASVIAAMKSKYGAEEGRLQWAVADMSELSTQFKSRKFSSILDKGALDAVVADGGDSWSPPDATLARGLQCVEEMSKVL 480
            Y  + YW+ RFE E   +WLA    V  L+  ++         +LVVGCGNS  S  L  E     + + D+S+ VI  M++KYG     ++W V DM +L  QF    F  ++DK A+DA++ +GGD+WSP    LA   +    ++ VL
Sbjct:   12 YKKQAYWDSRFESEESYDWLARYNDVAALVEQYVNPQA----RILVVGCGNSTFSIDLY-EAGYTNITNLDFSSVVIERMQAKYGESHPLMKWVVGDMLKLPEQFAPSSFDVVIDKAAMDALMVEGGDTWSPAQHLLASAHEMCSGITSVL 157          
BLAST of mRNA_M-pyrifera_M_contig93308.21548.1 vs. uniprot
Match: A0A6H5KV70_9PHAE (Methyltransf_25 domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5KV70_9PHAE)

HSP 1 Score: 101 bits (251), Expect = 9.530e-23
Identity = 61/155 (39.35%), Postives = 92/155 (59.35%), Query Frame = 1
Query:   25 LYVSKEYWEYRFEREGEKEWLADPETVLGLLRGWIGHVEGVGRD--VLVVGCGNSALSSALSREEWVK-EVLSTDYSASVIAAMKSKYGAEEGRLQWAVADMSELSTQFKSRKFSSILDKGALDAVVADGGDSWSPPDATLARGLQCVEEMSKVL 480
            +Y +KEYW+ RF  E   +WLA    +   L       E V RD  VLVVGCGNS LS+ +  + + + +++STD+SA VI  M++K+ A    L+W   DM  L+ +  S  F +++DK A+DA++ D GD WSP  AT+ +  +   E+S+VL
Sbjct:   93 VYKAKEYWDSRFSEEESYDWLASYADIAEHLH------EAVPRDARVLVVGCGNSGLSADMYDDGYRRVDMVSTDFSAVVIDKMRAKHLAARPGLRWEKMDMLALAAEDAS--FDAVVDKAAMDALMVDKGDPWSPDRATIEQSHRMCAEVSRVL 239          
BLAST of mRNA_M-pyrifera_M_contig93308.21548.1 vs. uniprot
Match: A0A1Y1ULD2_9TREE (S-adenosyl-L-methionine-dependent methyltransferase n=1 Tax=Kockovaella imperatae TaxID=4999 RepID=A0A1Y1ULD2_9TREE)

HSP 1 Score: 97.8 bits (242), Expect = 2.980e-22
Identity = 53/155 (34.19%), Postives = 89/155 (57.42%), Query Frame = 1
Query:   28 YVSKEYWEYRFEREGEK---EWLADPETVLGLLRGWIGHVEGVGRDVLVVGCGNSALSSALSREEWVKEVLSTDYSASVIAAMKSKYGAEEGRLQWAVADMSELSTQFKSRKFSSILDKGALDAVVADGGDSWSPPDATLARGLQCVEEMSKVLQ 483
            Y ++EYWE R+  E      +W   P  ++ ++R     + G    VL++GCGNSALS A+    W K +++ DYS  VI  M  ++ A++  ++W V D+ +L  QF+   F  I+DKG +DA++   GD W+PP+  +   + C +E+S+ L+
Sbjct:   16 YGTREYWEQRYTNEAPDTTFDWFLTPSYLIPIIREQTQDI-GTSARVLMLGCGNSALSEAMYEAGW-KNIVNIDYSEKVIQQMSERH-ADKPEMRWQVMDVLDL--QFEKESFDLIIDKGTMDAMLTTKGDPWNPPEKDV---IACTKEVSEALR 162          
BLAST of mRNA_M-pyrifera_M_contig93308.21548.1 vs. uniprot
Match: T0S8X2_SAPDV (Methyltransf_11 domain-containing protein n=3 Tax=Saprolegniaceae TaxID=4764 RepID=T0S8X2_SAPDV)

HSP 1 Score: 96.7 bits (239), Expect = 6.940e-22
Identity = 53/152 (34.87%), Postives = 84/152 (55.26%), Query Frame = 1
Query:   28 YVSKEYWEYRFEREGEKEWLADPETVLGLLRGWIGHVEGVGRDVLVVGCGNSALSSALSREEWVKEVLSTDYSASVIAAMKSKYGAEEGRLQWAVADMSELSTQFKSRKFSSILDKGALDAVVADGGDSWSPPDATLARGLQCVEEMSKVLQ 483
            Y ++ YW+ RF  E   +WLA    V  L+   + +V    R +L+VGCGNS  S  L    +   +++ D+S+ VI  M +KY      ++W V DM +LS  F +  F  ++DK A+DA++ +GGD+WSP +  LA+       + KVL+
Sbjct:    8 YKNQSYWDSRFASEESYDWLARYNDVADLI---VQYVPKSSR-ILIVGCGNSTFSVDLYDAGYTN-IVNLDFSSVVIERMSAKYTESRPLMRWVVGDMLKLSESFGASSFDVVIDKAAMDALMVEGGDTWSPAEHLLAQARDMCTGVQKVLK 154          
BLAST of mRNA_M-pyrifera_M_contig93308.21548.1 vs. uniprot
Match: A0A225WNN2_9STRA (Methyltransf_25 domain-containing protein n=1 Tax=Phytophthora megakarya TaxID=4795 RepID=A0A225WNN2_9STRA)

HSP 1 Score: 96.7 bits (239), Expect = 7.760e-22
Identity = 52/151 (34.44%), Postives = 82/151 (54.30%), Query Frame = 1
Query:   28 YVSKEYWEYRFEREGEKEWLADPETVLGLLRGWIGHVEGVGRDVLVVGCGNSALSSALSREEWVKEVLSTDYSASVIAAMKSKYGAEEGRLQWAVADMSELSTQFKSRKFSSILDKGALDAVVADGGDSWSPPDATLARGLQCVEEMSKVL 480
            Y  K YW+ RF++E   +WLA  E V  LL  ++   +     +L+VGCGNS  S  + +  +   + + D+S  VI  M +K+  E   ++W  ADM++L   F S  F  ++DK A+DA++ D GD WSP DA + +       ++ VL
Sbjct:   12 YKEKGYWDSRFDKEETYDWLARYEDVADLLDKYVRPSD----RILMVGCGNSTFSIDMYQAGF-HNITNIDFSKVVIERMSAKHSEEMPEMKWLEADMTKLGEVFTSESFDVVIDKAAMDALMCDEGDVWSPSDAVIEQAAAMCNGITSVL 157          
BLAST of mRNA_M-pyrifera_M_contig93308.21548.1 vs. uniprot
Match: A0A0P1ABL8_PLAHL (S-adenosyl-l-methionine-dependent methyltransferase n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1ABL8_PLAHL)

HSP 1 Score: 95.9 bits (237), Expect = 1.610e-21
Identity = 52/151 (34.44%), Postives = 80/151 (52.98%), Query Frame = 1
Query:   28 YVSKEYWEYRFEREGEKEWLADPETVLGLLRGWIGHVEGVGRDVLVVGCGNSALSSALSREEWVKEVLSTDYSASVIAAMKSKYGAEEGRLQWAVADMSELSTQFKSRKFSSILDKGALDAVVADGGDSWSPPDATLARGLQCVEEMSKVL 480
            Y  K YW+ RF  E   +WLA  E +  LL  ++   +     +L+VGCGNS  S  +    +   + + D+S  VIA MK+KY  +   ++W  ADM++LS  FK   F  ++DK A+DA++ D GD WSP +  +         ++ VL
Sbjct:   12 YKEKSYWDSRFYSEDSYDWLARYENLAELLTKYVRPSD----RILMVGCGNSTFSIEMYNAGF-HNITNIDFSGIVIARMKAKYKDKMPEMKWKEADMTKLSETFKFGSFDVVIDKAAMDALMCDEGDVWSPSETVIKEAAAMCSGIASVL 157          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig93308.21548.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
W4FG40_9STRA1.710e-2435.19Methyltransf_25 domain-containing protein n=1 Tax=... [more]
A0A024UL85_9STRA2.420e-2437.09Methyltransf_25 domain-containing protein n=1 Tax=... [more]
A0A485KM56_9STRA1.760e-2335.76Aste57867_9023 protein n=1 Tax=Aphanomyces stellat... [more]
A0A1W0A6C7_9STRA2.420e-2334.81Methyltransf_11 domain-containing protein n=1 Tax=... [more]
A0A6G0XDR0_9STRA5.770e-2335.76Methyltransf_25 domain-containing protein n=1 Tax=... [more]
A0A6H5KV70_9PHAE9.530e-2339.35Methyltransf_25 domain-containing protein n=2 Tax=... [more]
A0A1Y1ULD2_9TREE2.980e-2234.19S-adenosyl-L-methionine-dependent methyltransferas... [more]
T0S8X2_SAPDV6.940e-2234.87Methyltransf_11 domain-containing protein n=3 Tax=... [more]
A0A225WNN2_9STRA7.760e-2234.44Methyltransf_25 domain-containing protein n=1 Tax=... [more]
A0A0P1ABL8_PLAHL1.610e-2134.44S-adenosyl-l-methionine-dependent methyltransferas... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig93308contigM-pyrifera_M_contig93308:3..488 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score103.6
Seed ortholog evalue8.8e-20
Seed eggNOG ortholog2880.D7FJW5
Preferred nameECE2
KEGG koko:K01415
Hectar predicted targeting categoryother localisation
GOsGO:0000139,GO:0003674,GO:0003824,GO:0004175,GO:0004222,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005794,GO:0005802,GO:0006464,GO:0006479,GO:0006508,GO:0006518,GO:0006807,GO:0007275,GO:0007399,GO:0007417,GO:0007420,GO:0007507,GO:0008150,GO:0008152,GO:0008168,GO:0008170,GO:0008213,GO:0008233,GO:0008237,GO:0008276,GO:0008757,GO:0009987,GO:0010002,GO:0010467,GO:0010817,GO:0012505,GO:0012506,GO:0016020,GO:0016278,GO:0016279,GO:0016485,GO:0016486,GO:0016740,GO:0016741,GO:0016787,GO:0018022,GO:0018193,GO:0018205,GO:0019538,GO:0030133,GO:0030154,GO:0030658,GO:0030659,GO:0031090,GO:0031410,GO:0031982,GO:0031984,GO:0032259,GO:0032501,GO:0032502,GO:0034641,GO:0035051,GO:0036211,GO:0042445,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0043414,GO:0043603,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044431,GO:0044433,GO:0044444,GO:0044446,GO:0044464,GO:0048513,GO:0048731,GO:0048856,GO:0048863,GO:0048869,GO:0051604,GO:0060322,GO:0065007,GO:0065008,GO:0070011,GO:0071704,GO:0072359,GO:0097708,GO:0098588,GO:0098791,GO:0098805,GO:0140096,GO:1901564
EggNOG free text desc.methyltransferase activity
EggNOG OGsKOG2352@1,KOG2352@2759
Ec32 ortholog descriptionS-adenosyl-L-methionine-dependent methyltransferase
Ec32 orthologEc-10_000720.1
EC3.4.24.71
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko01002,ko04147
Exons1
Model size486
Cds size486
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816845.1712542-CDS-M-pyrifera_M_contig93308:2..4881622816845.1712542-CDS-M-pyrifera_M_contig93308:2..488Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93308 3..488 -
1692278212.2233958-CDS-M-pyrifera_M_contig93308:2..4881692278212.2233958-CDS-M-pyrifera_M_contig93308:2..488Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93308 3..488 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig93308.21548.1prot_M-pyrifera_M_contig93308.21548.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig93308 3..488 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig93308.21548.1

>prot_M-pyrifera_M_contig93308.21548.1 ID=prot_M-pyrifera_M_contig93308.21548.1|Name=mRNA_M-pyrifera_M_contig93308.21548.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=162bp
VTPKHGSALYVSKEYWEYRFEREGEKEWLADPETVLGLLRGWIGHVEGVG
RDVLVVGCGNSALSSALSREEWVKEVLSTDYSASVIAAMKSKYGAEEGRL
QWAVADMSELSTQFKSRKFSSILDKGALDAVVADGGDSWSPPDATLARGL
QCVEEMSKVLQE
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mRNA from alignment at M-pyrifera_M_contig93308:3..488-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig93308.21548.1 ID=mRNA_M-pyrifera_M_contig93308.21548.1|Name=mRNA_M-pyrifera_M_contig93308.21548.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=486bp|location=Sequence derived from alignment at M-pyrifera_M_contig93308:3..488- (Macrocystis pyrifera P11B4 male)
GTGACGCCAAAGCATGGCTCAGCGTTGTATGTGTCAAAGGAATACTGGGA ATATCGGTTTGAGAGAGAAGGGGAAAAAGAATGGCTGGCTGATCCAGAGA CGGTGTTGGGTTTGTTGCGAGGTTGGATTGGTCATGTAGAGGGAGTTGGG AGGGATGTCTTGGTGGTAGGGTGTGGGAACAGTGCTTTGAGTAGTGCATT GTCACGGGAAGAGTGGGTGAAAGAGGTGCTATCTACCGATTATTCGGCAT CTGTGATCGCCGCTATGAAATCAAAGTATGGAGCTGAAGAAGGGAGATTG CAATGGGCTGTTGCGGATATGAGTGAACTGAGTACGCAGTTTAAGAGTCG CAAGTTTTCGTCCATCTTGGACAAAGGAGCGCTTGATGCAGTTGTTGCAG ATGGTGGAGATTCGTGGAGTCCCCCAGATGCCACTTTGGCGCGAGGACTG CAATGTGTGGAGGAAATGTCCAAGGTGCTGCAAGAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig93308:3..488-

>mRNA_M-pyrifera_M_contig93308.21548.1 ID=mRNA_M-pyrifera_M_contig93308.21548.1|Name=mRNA_M-pyrifera_M_contig93308.21548.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=972bp|location=Sequence derived from alignment at M-pyrifera_M_contig93308:3..488- (Macrocystis pyrifera P11B4 male)
GTGACGCCAAAGCATGGCTCAGCGTTGTATGTGTCAAAGGAATACTGGGA
ATATCGGTTTGAGAGAGAAGGGGAAAAAGAATGGCTGGCTGATCCAGAGA
CGGTGTTGGGTTTGTTGCGAGGTTGGATTGGTCATGTAGAGGGAGTTGGG
AGGGATGTCTTGGTGGTAGGGTGTGGGAACAGTGCTTTGAGTAGTGCATT
GTCACGGGAAGAGTGGGTGAAAGAGGTGCTATCTACCGATTATTCGGCAT
CTGTGATCGCCGCTATGAAATCAAAGTATGGAGCTGAAGAAGGGAGATTG
CAATGGGCTGTTGCGGATATGAGTGAACTGAGTACGCAGTTTAAGAGTCG
CAAGTTTTCGTCCATCTTGGACAAAGGAGCGCTTGATGCAGTTGTTGCAG
ATGGTGGAGATTCGTGGAGTCCCCCAGATGCCACTTTGGCGCGAGGACTG
CAATGTGTGGAGGAAATGTCCAAGGTGCTGCAAGAGGTGACGCCAAAGCA
TGGCTCAGCGTTGTATGTGTCAAAGGAATACTGGGAATATCGGTTTGAGA
GAGAAGGGGAAAAAGAATGGCTGGCTGATCCAGAGACGGTGTTGGGTTTG
TTGCGAGGTTGGATTGGTCATGTAGAGGGAGTTGGGAGGGATGTCTTGGT
GGTAGGGTGTGGGAACAGTGCTTTGAGTAGTGCATTGTCACGGGAAGAGT
GGGTGAAAGAGGTGCTATCTACCGATTATTCGGCATCTGTGATCGCCGCT
ATGAAATCAAAGTATGGAGCTGAAGAAGGGAGATTGCAATGGGCTGTTGC
GGATATGAGTGAACTGAGTACGCAGTTTAAGAGTCGCAAGTTTTCGTCCA
TCTTGGACAAAGGAGCGCTTGATGCAGTTGTTGCAGATGGTGGAGATTCG
TGGAGTCCCCCAGATGCCACTTTGGCGCGAGGACTGCAATGTGTGGAGGA
AATGTCCAAGGTGCTGCAAGAG
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