mRNA_M-pyrifera_M_contig93300.21544.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig93300.21544.1
Unique NamemRNA_M-pyrifera_M_contig93300.21544.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig93300.21544.1 vs. uniprot
Match: UPI00166A48F8 (SMP-30/gluconolactonase/LRE family protein n=1 Tax=Emticicia aquatilis TaxID=1537369 RepID=UPI00166A48F8)

HSP 1 Score: 95.9 bits (237), Expect = 1.540e-20
Identity = 70/162 (43.21%), Postives = 90/162 (55.56%), Query Frame = 1
Query:   13 STGGIFELDLDTMEVYEVPLVGFCTEENTF--HPHGISIFRDTPEGPTLLFIVNHRNPGLGDQVLVFQFNAEERTLTHVDTIEDVLFVSINDVVAVGLQQFFVPNDSGYPRDSLWRLSAFLFPFTSGSTVVYYDGELASVAIKAGEIAFANGINVSLGGKYL 492
              G IF L+  T E    P+    TE+ TF  HPHGIS++R T EG   LF++NHR  G  D V VF F   + +L H+++I   LFVS ND+VAVG   F++ ND    +   WR    L     G+ V YYDG+ A V   A    +ANGIN+SL GK L
Sbjct:   73 KNGAIFLLN--TKENTPKPIK--LTEKVTFDFHPHGISLYR-TLEGAKRLFVINHRKKG--DYVEVFDFT--DSSLVHLESITSSLFVSPNDIVAVGEHSFYLTNDHDEEKSD-WRSKKDLLQIAMGN-VCYYDGQRAKVV--ADGFLYANGINISLDGKKL 221          
BLAST of mRNA_M-pyrifera_M_contig93300.21544.1 vs. uniprot
Match: A0A2D0RVV1_ICTPU (Paraoxonase n=2 Tax=Ictaluridae TaxID=7996 RepID=A0A2D0RVV1_ICTPU)

HSP 1 Score: 95.1 bits (235), Expect = 3.340e-20
Identity = 58/132 (43.94%), Postives = 76/132 (57.58%), Query Frame = 1
Query:   97 TFHPHGISIFRDTPEGPTLLFIVNHRNPGLGDQVLVFQFNAEERTLTHVDTIEDVLFVSINDVVAVGLQQFFVPNDSGYPRDSLWRLSAFLFPFTSGSTVVYYDGELASVAIKAGEIAFANGINVSLGGKYL 492
            +F PHGIS++ D  +G   LF+VNH  P    QV +FQ+  EE TL HV TI+  L  ++ND+VAVG++ F+  ND  +  D L  L   L  F     VVYY  E   VA  AG +  ANGIN+S   K+L
Sbjct:  109 SFGPHGISLYTDEEDGSRYLFVVNH--PHKNSQVEIFQYVKEENTLVHVKTIKHELLHNVNDIVAVGVENFYATNDHYFTTDYLKPLEMLLSLFWCD--VVYYSPEAVHVA--AGGLFSANGINISPDKKHL 234          
BLAST of mRNA_M-pyrifera_M_contig93300.21544.1 vs. uniprot
Match: A0A5N5Q5A0_PANHP (Paraoxonase n=1 Tax=Pangasianodon hypophthalmus TaxID=310915 RepID=A0A5N5Q5A0_PANHP)

HSP 1 Score: 93.6 bits (231), Expect = 1.240e-19
Identity = 55/132 (41.67%), Postives = 78/132 (59.09%), Query Frame = 1
Query:   97 TFHPHGISIFRDTPEGPTLLFIVNHRNPGLGDQVLVFQFNAEERTLTHVDTIEDVLFVSINDVVAVGLQQFFVPNDSGYPRDSLWRLSAFLFPFTSGSTVVYYDGELASVAIKAGEIAFANGINVSLGGKYL 492
            +F PHGIS++ D  +G   LF+VNH  P    QV +FQ+  EE TL H+ TI+  L  ++ND+VAVG++ F+  ND  +  + L  L A L  +     VVYY  E  +V + AG +  ANGIN+S   K+L
Sbjct:  109 SFGPHGISVYTDEEDGSRYLFVVNH--PHENSQVEIFQYVEEESTLVHMKTIKHELLHNVNDIVAVGVENFYATNDHYFTNEYLMPLEALLSLYWCN--VVYYSPE--AVHVVAGGLFSANGINISPDKKHL 234          
BLAST of mRNA_M-pyrifera_M_contig93300.21544.1 vs. uniprot
Match: UPI000575FB11 (serum paraoxonase/arylesterase 2-like n=1 Tax=Esox lucius TaxID=8010 RepID=UPI000575FB11)

HSP 1 Score: 92.0 bits (227), Expect = 4.580e-19
Identity = 62/168 (36.90%), Postives = 92/168 (54.76%), Query Frame = 1
Query:    1 PVRISTGGIFELDLDTMEVYEVPL-VGFCTEENTFHPHGISIFRDTPEGPTLLFIVNHRNPGLGDQVLVFQFNAEERTLTHVDTIEDVLFVSINDVVAVGLQQFFVPNDSGYPRDSLWRLSAFL-FPFTSGSTVVYYDGELASVAIKAGEIAF--ANGINVSLGGKYL 492
            P     G I+ LDL    +  V L +    ++N+F+PHGIS++ D  +    LF+VNH  P    QV +F+F  ++ T+ H  T++  L  S+ND+VAVG + F+  ND  YP + L  L+ FL  P+   + VVYY  E    A+K     F  ANG+N+S   +YL
Sbjct:   75 PSCAEPGKIYTLDLLDPRLTPVELAINGELDQNSFNPHGISVYTDETDNTIHLFVVNHPQPHTS-QVEIFRFVEDDNTIVHRRTVKHDLLHSVNDIVAVGEESFYATNDHSYPSEVLHWLTVFLGLPW---ARVVYYSPE----AVKEVGDGFMSANGVNMSPDKRYL 234          
BLAST of mRNA_M-pyrifera_M_contig93300.21544.1 vs. uniprot
Match: PON1_MOUSE (Serum paraoxonase/arylesterase 1 n=14 Tax=Muridae TaxID=10066 RepID=PON1_MOUSE)

HSP 1 Score: 91.7 bits (226), Expect = 6.350e-19
Identity = 63/162 (38.89%), Postives = 87/162 (53.70%), Query Frame = 1
Query:   19 GGIFELDLDTME--VYEVPLVGFCTEENTFHPHGISIFRDTPEGPTLLFIVNHRNPGLGDQVLVFQFNAEERTLTHVDTIEDVLFVSINDVVAVGLQQFFVPNDSGYPRDSL--WRLSAFLFPFTSGSTVVYYDGELASVAIKAGEIAFANGINVSLGGKYL 492
            G I  +DL+  E  V E+ ++G   + ++F+PHGIS F D  +    L +VNH  P     V VF+F  EER+L H+ TI   L  SIND+ A+G + F+  ND  +    L  W +   L    S S VVYY  +   V + A    FANGI +SL GKY+
Sbjct:   83 GKILLMDLNKKEPAVSELEIIGNTLDISSFNPHGISTFTDE-DNTVYLLVVNH--PDSSSTVEVFKFQEEERSLLHLKTITHELLPSINDIAAIGPESFYATNDHYFADPYLRSWEMYLGL----SWSNVVYYSPD--KVQVVAEGFDFANGIGISLDGKYV 235          
BLAST of mRNA_M-pyrifera_M_contig93300.21544.1 vs. uniprot
Match: F6NHB4_DANRE (Paraoxonase 1 n=2 Tax=Danio rerio TaxID=7955 RepID=F6NHB4_DANRE)

HSP 1 Score: 90.9 bits (224), Expect = 1.230e-18
Identity = 52/136 (38.24%), Postives = 79/136 (58.09%), Query Frame = 1
Query:   88 EENTFHPHGISIFRDTPEGPTLLFIVNHRNPGLGDQVLVFQFNAEERTLTHVDTIEDVLFVSINDVVAVGLQQFFVPNDSGYPRDSLWRLSAFL-FPFTSGSTVVYYDGELASVAIKAGEIAFANGINVSLGGKYL 492
            +++TF+PHGIS++ D  +G   LF++NH  P    QV +F+F  +E  L ++ TIE  L  S+ND+VAVG + F+  ND  +  + L  +  F   P+     V+YY  E  +V + A     ANGIN+SL  +YL
Sbjct:  107 DQDTFNPHGISVYTDDKDGTMYLFVINH--PRGNSQVEIFEFVKDEHALKYIKTIEHELLHSVNDIVAVGTESFYATNDHYFSNEILKIVENFFTLPWCD---VIYYSPE--TVQVVADGFLLANGINLSLDKRYL 235          
BLAST of mRNA_M-pyrifera_M_contig93300.21544.1 vs. uniprot
Match: A0A556TZ19_BAGYA (Paraoxonase n=1 Tax=Bagarius yarrelli TaxID=175774 RepID=A0A556TZ19_BAGYA)

HSP 1 Score: 90.9 bits (224), Expect = 1.670e-18
Identity = 56/132 (42.42%), Postives = 76/132 (57.58%), Query Frame = 1
Query:   97 TFHPHGISIFRDTPEGPTLLFIVNHRNPGLGDQVLVFQFNAEERTLTHVDTIEDVLFVSINDVVAVGLQQFFVPNDSGYPRDSLWRLSAFLFPFTSGSTVVYYDGELASVAIKAGEIAFANGINVSLGGKYL 492
            +F PHGISI+ D  +G   LF+VNH  P    Q+ +FQ+  EE TL H+ TI+  L  ++ND+VAVG++ FF  ND  +  + L  L A LF       VVYY  E+  V   AG    ANG+N+S   K+L
Sbjct:   90 SFGPHGISIYTDEKDGSRYLFVVNH--PHENSQIEIFQYIEEENTLLHLKTIKHKLLHNVNDIVAVGVENFFATNDHYFTNEPLKLLEA-LFSL-HWCNVVYYSPEVVQVV--AGGFFGANGVNMSPDKKHL 215          
BLAST of mRNA_M-pyrifera_M_contig93300.21544.1 vs. uniprot
Match: A0A6G0IB09_LARCR (Paraoxonase n=2 Tax=Larimichthys crocea TaxID=215358 RepID=A0A6G0IB09_LARCR)

HSP 1 Score: 90.1 bits (222), Expect = 2.340e-18
Identity = 57/134 (42.54%), Postives = 79/134 (58.96%), Query Frame = 1
Query:   94 NTFHPHGISIFRDTPEGPTLLFIVNHRNPGLGDQVLVFQFNAEERTLTHVDTIEDVLFVSINDVVAVGLQQFFVPNDSGYPRDSLWRLSAFL-FPFTSGSTVVYYDGELASVAIKAGEIAFANGINVSLGGKYL 492
            ++F+PHGIS++ D  +    LF+VNH  P    QV VF F  +E TL H+ TI   L  S+ND+VAVG++ F+  ND  +  D+L  LS FL  PF     VVYY  +   V + A  I+ ANGIN+S   +Y+
Sbjct:  109 SSFNPHGISVYTDEADDSVYLFVVNH--PQNKSQVEVFHF-VQENTLVHLKTITHPLLHSVNDIVAVGVENFYASNDRFFLNDALQFLSIFLGVPFCQ---VVYYSPK--EVRVAADGISSANGINISPDKQYI 234          
BLAST of mRNA_M-pyrifera_M_contig93300.21544.1 vs. uniprot
Match: A0A8I5ZN72_RAT (Paraoxonase n=1 Tax=Rattus norvegicus TaxID=10116 RepID=A0A8I5ZN72_RAT)

HSP 1 Score: 89.4 bits (220), Expect = 3.450e-18
Identity = 63/162 (38.89%), Postives = 87/162 (53.70%), Query Frame = 1
Query:   19 GGIFELDLDTME--VYEVPLVGFCTEENTFHPHGISIFRDTPEGPTLLFIVNHRNPGLGDQVLVFQFNAEERTLTHVDTIEDVLFVSINDVVAVGLQQFFVPNDSGYPRDSL--WRLSAFLFPFTSGSTVVYYDGELASVAIKAGEIAFANGINVSLGGKYL 492
            G I  +DL+  E  V E+ ++G   + ++F+PHGIS F D  +    L +V+H  P     V VF+F  EER+L H+ TI   L  SIND+ AVG + F+  ND  +    L  W +   L    S S VVYY  +   V + A    FANGI +SL GKY+
Sbjct:   83 GKILLMDLNEKEPAVSELAIMGNTLDMSSFNPHGISTFIDE-DNTVYLLVVSH--PDSSSTVEVFKFQEEERSLLHLKTITHELLPSINDIAAVGPESFYATNDHYFADPYLRSWEMYLGL----SWSNVVYYSPD--KVRVVADGFDFANGIGISLDGKYV 235          
BLAST of mRNA_M-pyrifera_M_contig93300.21544.1 vs. uniprot
Match: PON1_RAT (Serum paraoxonase/arylesterase 1 n=4 Tax=Tetrapoda TaxID=32523 RepID=PON1_RAT)

HSP 1 Score: 89.4 bits (220), Expect = 4.490e-18
Identity = 63/162 (38.89%), Postives = 87/162 (53.70%), Query Frame = 1
Query:   19 GGIFELDLDTME--VYEVPLVGFCTEENTFHPHGISIFRDTPEGPTLLFIVNHRNPGLGDQVLVFQFNAEERTLTHVDTIEDVLFVSINDVVAVGLQQFFVPNDSGYPRDSL--WRLSAFLFPFTSGSTVVYYDGELASVAIKAGEIAFANGINVSLGGKYL 492
            G I  +DL+  E  V E+ ++G   + ++F+PHGIS F D  +    L +V+H  P     V VF+F  EER+L H+ TI   L  SIND+ AVG + F+  ND  +    L  W +   L    S S VVYY  +   V + A    FANGI +SL GKY+
Sbjct:   83 GKILLMDLNEKEPAVSELAIMGNTLDMSSFNPHGISTFIDE-DNTVYLLVVSH--PDSSSTVEVFKFQEEERSLLHLKTITHELLPSINDIAAVGPESFYATNDHYFADPYLRSWEMYLGL----SWSNVVYYSPD--KVRVVADGFDFANGIGISLDGKYV 235          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig93300.21544.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI00166A48F81.540e-2043.21SMP-30/gluconolactonase/LRE family protein n=1 Tax... [more]
A0A2D0RVV1_ICTPU3.340e-2043.94Paraoxonase n=2 Tax=Ictaluridae TaxID=7996 RepID=A... [more]
A0A5N5Q5A0_PANHP1.240e-1941.67Paraoxonase n=1 Tax=Pangasianodon hypophthalmus Ta... [more]
UPI000575FB114.580e-1936.90serum paraoxonase/arylesterase 2-like n=1 Tax=Esox... [more]
PON1_MOUSE6.350e-1938.89Serum paraoxonase/arylesterase 1 n=14 Tax=Muridae ... [more]
F6NHB4_DANRE1.230e-1838.24Paraoxonase 1 n=2 Tax=Danio rerio TaxID=7955 RepID... [more]
A0A556TZ19_BAGYA1.670e-1842.42Paraoxonase n=1 Tax=Bagarius yarrelli TaxID=175774... [more]
A0A6G0IB09_LARCR2.340e-1842.54Paraoxonase n=2 Tax=Larimichthys crocea TaxID=2153... [more]
A0A8I5ZN72_RAT3.450e-1838.89Paraoxonase n=1 Tax=Rattus norvegicus TaxID=10116 ... [more]
PON1_RAT4.490e-1838.89Serum paraoxonase/arylesterase 1 n=4 Tax=Tetrapoda... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig93300contigM-pyrifera_M_contig93300:166..755 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score95.1
Seed ortholog evalue3.2e-17
Seed eggNOG ortholog8081.XP_008430663.1
Preferred namePON2
KEGG koko:K01045
KEGG Pathwayko01120,map01120
Hectar predicted targeting categoryother localisation
GOsGO:0000323,GO:0003674,GO:0003824,GO:0004064,GO:0005488,GO:0005515,GO:0005575,GO:0005576,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005739,GO:0005764,GO:0005773,GO:0005886,GO:0006082,GO:0006629,GO:0006631,GO:0006690,GO:0006725,GO:0006950,GO:0006979,GO:0008150,GO:0008152,GO:0009056,GO:0009636,GO:0009987,GO:0016020,GO:0016787,GO:0016788,GO:0019372,GO:0019439,GO:0019752,GO:0032787,GO:0042221,GO:0042802,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043436,GO:0044237,GO:0044238,GO:0044248,GO:0044255,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0046573,GO:0050896,GO:0052689,GO:0071704,GO:0071944,GO:0102007,GO:1901568
EggNOG free text desc.paraoxonase
EggNOG OGs2CHYE@1,2QUCT@2759,38VB8@33154,3BIJH@33208,3CWPF@33213,47ZVY@7711,48Z15@7742,4A1RA@7898
EC3.1.1.2,3.1.8.1
COG Functional cat.S
Best tax levelActinopterygii
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko04147
Exons2
Model size492
Cds size492
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816845.0265207-CDS-M-pyrifera_M_contig93300:165..3981622816845.0265207-CDS-M-pyrifera_M_contig93300:165..398Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93300 166..398 +
1692278212.1561832-CDS-M-pyrifera_M_contig93300:165..3981692278212.1561832-CDS-M-pyrifera_M_contig93300:165..398Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93300 166..398 +
1622816845.0374207-CDS-M-pyrifera_M_contig93300:496..7551622816845.0374207-CDS-M-pyrifera_M_contig93300:496..755Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93300 497..755 +
1692278212.1688895-CDS-M-pyrifera_M_contig93300:496..7551692278212.1688895-CDS-M-pyrifera_M_contig93300:496..755Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93300 497..755 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig93300.21544.1prot_M-pyrifera_M_contig93300.21544.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig93300 166..755 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig93300.21544.1

>prot_M-pyrifera_M_contig93300.21544.1 ID=prot_M-pyrifera_M_contig93300.21544.1|Name=mRNA_M-pyrifera_M_contig93300.21544.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=164bp
PVRISTGGIFELDLDTMEVYEVPLVGFCTEENTFHPHGISIFRDTPEGPT
LLFIVNHRNPGLGDQVLVFQFNAEERTLTHVDTIEDVLFVSINDVVAVGL
QQFFVPNDSGYPRDSLWRLSAFLFPFTSGSTVVYYDGELASVAIKAGEIA
FANGINVSLGGKYL
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mRNA from alignment at M-pyrifera_M_contig93300:166..755+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig93300.21544.1 ID=mRNA_M-pyrifera_M_contig93300.21544.1|Name=mRNA_M-pyrifera_M_contig93300.21544.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=590bp|location=Sequence derived from alignment at M-pyrifera_M_contig93300:166..755+ (Macrocystis pyrifera P11B4 male)
CCAGTCAGAATATCTACGGGAGGGATCTTCGAGCTTGATTTGGACACCAT GGAAGTCTACGAGGTTCCTTTGGTTGGGTTCTGCACCGAAGAGAACACTT TCCACCCGCACGGCATTTCCATCTTCCGGGACACTCCGGAAGGGCCCACC CTCTTGTTCATTGTCAACCACCGCAACCCTGGCCTTGGAGACCAAGTGCT TGTATTCCAGTTCAACGCCGAGGAGCGAACTCTGTAAGTACCACCATGAT CCCGCATGTATCTCTCTCTTTCACTCGCCGCACACGCTACACTCTCTGTT TCCCTCCAGTGTGAACTCTGACACCATGGAGGACGCACGTGGATACGATC GAAGACGTGCTGTTCGTCTCCATCAACGACGTGGTGGCAGTTGGCCTGCA GCAGTTCTTCGTCCCCAACGACAGCGGCTACCCGAGGGACTCACTTTGGC GGCTGTCCGCGTTCCTCTTCCCCTTCACCTCGGGCTCCACCGTCGTTTAC TACGACGGAGAGCTGGCCAGTGTGGCAATCAAGGCTGGAGAGATTGCGTT CGCCAATGGCATCAACGTCTCCCTTGGGGGCAAGTACCTC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig93300:166..755+

>mRNA_M-pyrifera_M_contig93300.21544.1 ID=mRNA_M-pyrifera_M_contig93300.21544.1|Name=mRNA_M-pyrifera_M_contig93300.21544.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=984bp|location=Sequence derived from alignment at M-pyrifera_M_contig93300:166..755+ (Macrocystis pyrifera P11B4 male)
CCAGTCAGAATATCTACGGGAGGGATCTTCGAGCTTGATTTGGACACCAT
GGAAGTCTACGAGGTTCCTTTGGTTGGGTTCTGCACCGAAGAGAACACTT
TCCACCCGCACGGCATTTCCATCTTCCGGGACACTCCGGAAGGGCCCACC
CTCTTGTTCATTGTCAACCACCGCAACCCTGGCCTTGGAGACCAAGTGCT
TGTATTCCAGTTCAACGCCGAGGAGCGAACTCTCCAGTCAGAATATCTAC
GGGAGGGATCTTCGAGCTTGATTTGGACACCATGGAAGTCTACGAGGTTC
CTTTGGTTGGGTTCTGCACCGAAGAGAACACTTTCCACCCGCACGGCATT
TCCATCTTCCGGGACACTCCGGAAGGGCCCACCCTCTTGTTCATTGTCAA
CCACCGCAACCCTGGCCTTGGAGACCAAGTGCTTGTATTCCAGTTCAACG
CCGAGGAGCGAACTCTGACGCACGTGGATACGATCGAAGACGTGCTGTTC
GTCTCCATCAACGACGTGGTGGCAGTTGGCCTGCAGCAGTTCTTCGTCCC
CAACGACAGCGGCTACCCGAGGGACTCACTTTGGCGGCTGTCCGCGTTCC
TCTTCCCCTTCACCTCGGGCTCCACCGTCGTTTACTACGACGGAGAGCTG
GCCAGTGTGGCAATCAAGGCTGGAGAGATTGCGTTCGCCAATGGCATCAA
CGTCTCCCTTGGGGGCAAGTACCTCGACGCACGTGGATACGATCGAAGAC
GTGCTGTTCGTCTCCATCAACGACGTGGTGGCAGTTGGCCTGCAGCAGTT
CTTCGTCCCCAACGACAGCGGCTACCCGAGGGACTCACTTTGGCGGCTGT
CCGCGTTCCTCTTCCCCTTCACCTCGGGCTCCACCGTCGTTTACTACGAC
GGAGAGCTGGCCAGTGTGGCAATCAAGGCTGGAGAGATTGCGTTCGCCAA
TGGCATCAACGTCTCCCTTGGGGGCAAGTACCTC
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