mRNA_M-pyrifera_M_contig93081.21511.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig93081.21511.1
Unique NamemRNA_M-pyrifera_M_contig93081.21511.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig93081.21511.1 vs. uniprot
Match: K3X2N3_GLOUD (PCI domain-containing protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3X2N3_GLOUD)

HSP 1 Score: 163 bits (413), Expect = 6.760e-46
Identity = 77/151 (50.99%), Postives = 113/151 (74.83%), Query Frame = 1 
Query:    1 LDSPDVRAVLAKEEMADLKALLHGFYECRYADFFAALIRIAPVVTRDRYLSQHVPFLVRELRVRAYRQFLDSYRSVTLDGMAEQFGVTTDFVDKELSRFVAAKRVHAQIDLIRGIVETTRPEDKDKRFADVIRKGDAVLNRLQRLARTLHV 453
            +DSP++ AV+  +E+  L   L+G YEC Y  FF A++ I P + RD+YLS HV F+ RELRV AY QFL++YRSVT+  MA  FGV+ +F+D EL+RF+AA R++A+ID + G++ET RP+ K+ ++ D I+KGD +LNR+Q+LAR ++V
Sbjct:  270 VDSPEILAVI--DEIPCLSDFLNGLYECNYKRFFTAMVDIQPYILRDKYLSTHVRFMYRELRVLAYSQFLEAYRSVTIQSMATAFGVSVEFLDTELARFIAAGRLNAKIDKVAGVIETNRPDAKNAQYQDAIKKGDLLLNRIQKLARVINV 418
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BLAST of mRNA_M-pyrifera_M_contig93081.21511.1 vs. uniprot
Match: A0A484EBG3_BRELC (PCI domain-containing protein n=3 Tax=Peronosporaceae TaxID=4777 RepID=A0A484EBG3_BRELC)

HSP 1 Score: 160 bits (405), Expect = 9.310e-45
Identity = 77/151 (50.99%), Postives = 113/151 (74.83%), Query Frame = 1 
Query:    1 LDSPDVRAVLAKEEMADLKALLHGFYECRYADFFAALIRIAPVVTRDRYLSQHVPFLVRELRVRAYRQFLDSYRSVTLDGMAEQFGVTTDFVDKELSRFVAAKRVHAQIDLIRGIVETTRPEDKDKRFADVIRKGDAVLNRLQRLARTLHV 453
            +DSP++ AV+  +E+  L   L+G Y+C Y  FF A++ I P + RD+YLS H  +L RELRV AY QFL+SYRSVT+  MA  FGV+ +F+DKEL+RF+AA R++A+ID + G++ET RP+ K+ ++ D I+KGD +LNR+Q+LAR ++V
Sbjct:  265 VDSPEILAVI--KEIPYLTDFLNGLYDCDYKKFFTAMVDIQPQILRDKYLSTHSRYLYRELRVLAYAQFLESYRSVTIASMAAAFGVSIEFLDKELARFIAAGRLNAKIDKVAGVIETNRPDAKNAQYQDAIKKGDLLLNRIQKLARVINV 413
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BLAST of mRNA_M-pyrifera_M_contig93081.21511.1 vs. uniprot
Match: A0A8K1CFM4_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CFM4_PYTOL)

HSP 1 Score: 159 bits (402), Expect = 2.980e-44
Identity = 76/151 (50.33%), Postives = 110/151 (72.85%), Query Frame = 1 
Query:    1 LDSPDVRAVLAKEEMADLKALLHGFYECRYADFFAALIRIAPVVTRDRYLSQHVPFLVRELRVRAYRQFLDSYRSVTLDGMAEQFGVTTDFVDKELSRFVAAKRVHAQIDLIRGIVETTRPEDKDKRFADVIRKGDAVLNRLQRLARTLHV 453
            +DSP++ AV+   E+  L   L+G Y+C Y  FF AL+ I P + RD+Y S H  FL RELRV AY QFL++YRSVT+  MA  FGV+ +F+D EL+RF+AA R++A+ID + G++ET RP+ K+ ++ D I+KGD +LNR+Q+LAR ++V
Sbjct:  272 VDSPEILAVI--NEIPFLSDFLNGLYDCNYKRFFTALVDIQPHILRDKYFSSHARFLYRELRVLAYSQFLEAYRSVTMSSMAGAFGVSVEFLDTELARFIAAGRLNAKIDKVAGVIETNRPDAKNAQYQDAIKKGDLLLNRIQKLARVINV 420
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BLAST of mRNA_M-pyrifera_M_contig93081.21511.1 vs. uniprot
Match: G5AAP4_PHYSP (PCI domain-containing protein n=16 Tax=Phytophthora TaxID=4783 RepID=G5AAP4_PHYSP)

HSP 1 Score: 159 bits (401), Expect = 4.260e-44
Identity = 76/151 (50.33%), Postives = 111/151 (73.51%), Query Frame = 1 
Query:    1 LDSPDVRAVLAKEEMADLKALLHGFYECRYADFFAALIRIAPVVTRDRYLSQHVPFLVRELRVRAYRQFLDSYRSVTLDGMAEQFGVTTDFVDKELSRFVAAKRVHAQIDLIRGIVETTRPEDKDKRFADVIRKGDAVLNRLQRLARTLHV 453
            +DSP++ AV+   E+  L   L+G Y+C Y  FF A++ I P + RD+YLS H  FL RELRV AY QFL++YRSVT+  MA  FGV+ +F+D EL+RF+AA R++A+ID + G++ET RP+ K+ ++ D I+KGD +LNR+Q+LAR ++V
Sbjct:  273 VDSPEILAVI--NEIPCLTDFLNGLYDCNYKRFFTAMVDIQPYILRDKYLSTHSRFLYRELRVLAYGQFLEAYRSVTIASMATAFGVSVEFLDNELARFIAAGRLNAKIDKVAGVIETNRPDAKNAQYQDAIKKGDLLLNRIQKLARVINV 421
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BLAST of mRNA_M-pyrifera_M_contig93081.21511.1 vs. uniprot
Match: A0A5D6XJZ2_9STRA (PCI domain-containing protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XJZ2_9STRA)

HSP 1 Score: 161 bits (407), Expect = 4.750e-44
Identity = 76/151 (50.33%), Postives = 110/151 (72.85%), Query Frame = 1 
Query:    1 LDSPDVRAVLAKEEMADLKALLHGFYECRYADFFAALIRIAPVVTRDRYLSQHVPFLVRELRVRAYRQFLDSYRSVTLDGMAEQFGVTTDFVDKELSRFVAAKRVHAQIDLIRGIVETTRPEDKDKRFADVIRKGDAVLNRLQRLARTLHV 453
            +DSP++ AVL   ++  L   L+G YEC Y  FF A++ + P + RD+YLS HV F+ RELRV AY QFL++YRSVT+  MA  FGV  DF+D EL+RF+AA R++A+ID + G++ET RP+ K+ ++ D I+ GD +LNR+Q+LAR ++V
Sbjct:  301 VDSPEILAVL--NDIPHLADFLNGLYECNYKRFFTAMVELQPFILRDKYLSTHVRFMYRELRVLAYSQFLEAYRSVTIQSMATAFGVGVDFLDTELARFIAAGRLNAKIDKVAGVIETNRPDAKNAQYQDAIKNGDLLLNRIQKLARVINV 449
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BLAST of mRNA_M-pyrifera_M_contig93081.21511.1 vs. uniprot
Match: A0A5A8D0W1_CAFRO (PCI domain-containing protein n=3 Tax=Sar TaxID=2698737 RepID=A0A5A8D0W1_CAFRO)

HSP 1 Score: 158 bits (399), Expect = 7.180e-44
Identity = 76/151 (50.33%), Postives = 117/151 (77.48%), Query Frame = 1 
Query:    1 LDSPDVRAVLAKEEMADLKALLHGFYECRYADFFAALIRIAPVVTRDRYLSQHVPFLVRELRVRAYRQFLDSYRSVTLDGMAEQFGVTTDFVDKELSRFVAAKRVHAQIDLIRGIVETTRPEDKDKRFADVIRKGDAVLNRLQRLARTLHV 453
            +++PD+ +V A  ++ +   L+   ++CRYADFF AL  IAP ++RDR+L +HV   V+E+RVRAY QFL++YRSVTL+G+A  FGV+T F+D EL+RF+AA+R++A+I+ + GIVE+TRP+ K+ ++   +R+ DA+L R+QRLAR+L V
Sbjct:  265 VNAPDLLSVSA--QVPEAFTLVRALHDCRYADFFRALTAIAPALSRDRFLGRHVALYVKEMRVRAYSQFLEAYRSVTLEGIASAFGVSTSFIDAELARFIAARRLNAKINRMTGIVESTRPDSKNAKYESSLRRTDALLARVQRLARSLDV 413
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BLAST of mRNA_M-pyrifera_M_contig93081.21511.1 vs. uniprot
Match: M4BBE2_HYAAE (PCI domain-containing protein n=1 Tax=Hyaloperonospora arabidopsidis (strain Emoy2) TaxID=559515 RepID=M4BBE2_HYAAE)

HSP 1 Score: 156 bits (395), Expect = 2.150e-43
Identity = 75/151 (49.67%), Postives = 110/151 (72.85%), Query Frame = 1 
Query:    1 LDSPDVRAVLAKEEMADLKALLHGFYECRYADFFAALIRIAPVVTRDRYLSQHVPFLVRELRVRAYRQFLDSYRSVTLDGMAEQFGVTTDFVDKELSRFVAAKRVHAQIDLIRGIVETTRPEDKDKRFADVIRKGDAVLNRLQRLARTLHV 453
            +DSP + AV+  +E+  L   L G Y+C Y  FF A++ I P + RD+YLS H  +L RELRV AY QFL++YRSVT+  MA  FGV+ DF+D EL+RF+AA R++A+ID + G++ET RP+ K+ ++ + I+KGD +LNR+Q+LAR ++V
Sbjct:  252 VDSPKILAVI--KEIPFLTDFLSGIYDCNYKKFFTAMVDIQPYILRDKYLSTHSRYLYRELRVLAYAQFLEAYRSVTIASMAAAFGVSVDFLDTELARFIAAGRLNAKIDKVAGVIETNRPDAKNAQYQEAIKKGDLLLNRIQKLARVINV 400
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BLAST of mRNA_M-pyrifera_M_contig93081.21511.1 vs. uniprot
Match: A0A662XTJ5_9STRA (PCI domain-containing protein (Fragment) n=2 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662XTJ5_9STRA)

HSP 1 Score: 156 bits (395), Expect = 6.150e-43
Identity = 75/151 (49.67%), Postives = 110/151 (72.85%), Query Frame = 1 
Query:    1 LDSPDVRAVLAKEEMADLKALLHGFYECRYADFFAALIRIAPVVTRDRYLSQHVPFLVRELRVRAYRQFLDSYRSVTLDGMAEQFGVTTDFVDKELSRFVAAKRVHAQIDLIRGIVETTRPEDKDKRFADVIRKGDAVLNRLQRLARTLHV 453
            +DSP++ AV+   E+  L   L+G Y+C Y  FF A++ I P + +D+YLS H  FL RELRV AY QFL++YRSVT+  MA  FGV+  F+D EL+RF+AA R++A+ID + G++ET RP+ K+ ++ D I+KGD +LNR+Q+LAR ++V
Sbjct:  307 VDSPEILAVI--NEIPYLADFLNGLYDCDYKKFFGAMVDIQPYILQDKYLSTHARFLYRELRVLAYAQFLEAYRSVTIASMAAAFGVSVGFLDTELARFIAAGRLNAKIDKVAGVIETNRPDAKNAQYQDAIKKGDLLLNRIQKLARVINV 455
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BLAST of mRNA_M-pyrifera_M_contig93081.21511.1 vs. uniprot
Match: A0A6G0WDY7_9STRA (PCI domain-containing protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WDY7_9STRA)

HSP 1 Score: 155 bits (392), Expect = 6.890e-43
Identity = 73/151 (48.34%), Postives = 111/151 (73.51%), Query Frame = 1 
Query:    1 LDSPDVRAVLAKEEMADLKALLHGFYECRYADFFAALIRIAPVVTRDRYLSQHVPFLVRELRVRAYRQFLDSYRSVTLDGMAEQFGVTTDFVDKELSRFVAAKRVHAQIDLIRGIVETTRPEDKDKRFADVIRKGDAVLNRLQRLARTLHV 453
            +DS +V AV+   ++  L   L+G Y+C+Y  FF A++ + P V RD+YL+ H  F+ RELR+ AY QFL++YRSVT+  MA  FGV  +F+D ELSRF+AA R++A+ID + G++ET RP+ K+ ++ + I+KGDA+LNR+Q+LAR ++V
Sbjct:  259 VDSSEVLAVI--RDIPHLSDFLNGLYDCKYKQFFTAIVGLHPYVNRDKYLALHSRFIYRELRILAYAQFLEAYRSVTIQSMATAFGVGIEFLDTELSRFIAAGRLNAKIDKVAGVIETNRPDAKNAQYQETIKKGDALLNRIQKLARVINV 407
[Close]
BLAST of mRNA_M-pyrifera_M_contig93081.21511.1 vs. uniprot
Match: A0A4D9CYL3_9STRA (PCI domain-containing protein n=2 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9CYL3_9STRA)

HSP 1 Score: 155 bits (392), Expect = 7.910e-43
Identity = 76/147 (51.70%), Postives = 107/147 (72.79%), Query Frame = 1 
Query:    1 LDSPDVRAVLAKEEMADLKALLHGFYECRYADFFAALIRIAPVVTRDRYLSQHVPFLVRELRVRAYRQFLDSYRSVTLDGMAEQFGVTTDFVDKELSRFVAAKRVHAQIDLIRGIVETTRPEDKDKRFADVIRKGDAVLNRLQRLAR 441
            +D P+V AVL   E   L  LL+  Y+C Y  FF AL+ + P +TRDRY+S H+  LVRE+RV  Y QFL+SY+SV +  MA+ FG++ DF+D+ELSRF+AA R+ A+ID + G+VET RP+ K+ ++ +V+R GDA+LNRL +LAR
Sbjct:  266 VDGPEVIAVL--REKPALGRLLNSLYDCDYRAFFQALVEVTPELTRDRYMSPHLRLLVREMRVLVYSQFLESYKSVRVASMADAFGISVDFLDRELSRFIAAGRLSAKIDKVGGVVETRRPDSKNAQYLEVVRDGDALLNRLSKLAR 410
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig93081.21511.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
K3X2N3_GLOUD6.760e-4650.99PCI domain-containing protein n=1 Tax=Globisporang... [more]
A0A484EBG3_BRELC9.310e-4550.99PCI domain-containing protein n=3 Tax=Peronosporac... [more]
A0A8K1CFM4_PYTOL2.980e-4450.33Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
G5AAP4_PHYSP4.260e-4450.33PCI domain-containing protein n=16 Tax=Phytophthor... [more]
A0A5D6XJZ2_9STRA4.750e-4450.33PCI domain-containing protein n=1 Tax=Pythium bras... [more]
A0A5A8D0W1_CAFRO7.180e-4450.33PCI domain-containing protein n=3 Tax=Sar TaxID=26... [more]
M4BBE2_HYAAE2.150e-4349.67PCI domain-containing protein n=1 Tax=Hyaloperonos... [more]
A0A662XTJ5_9STRA6.150e-4349.67PCI domain-containing protein (Fragment) n=2 Tax=N... [more]
A0A6G0WDY7_9STRA6.890e-4348.34PCI domain-containing protein n=1 Tax=Aphanomyces ... [more]
A0A4D9CYL3_9STRA7.910e-4351.70PCI domain-containing protein n=2 Tax=Monodopsidac... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig93081contigM-pyrifera_M_contig93081:303..758 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score166.0
Seed ortholog evalue1.4e-38
Seed eggNOG ortholog65071.PYU1_T011482
Preferred nameRPN7
KEGG rclassRC00149
KEGG koko:K00134,ko:K03037,ko:K08869
KEGG ReactionR01061
KEGG Pathwayko00010,ko00710,ko01100,ko01110,ko01120,ko01130,ko01200,ko01230,ko03050,ko04066,ko05010,ko05169,map00010,map00710,map01100,map01110,map01120,map01130,map01200,map01230,map03050,map04066,map05010,map05169
KEGG ModuleM00001,M00002,M00003,M00165,M00166,M00308,M00341,M00552
Hectar predicted targeting categoryother localisation
GOsGO:0000502,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005737,GO:0005829,GO:0005886,GO:0006508,GO:0006511,GO:0006807,GO:0008150,GO:0008152,GO:0009056,GO:0009057,GO:0009987,GO:0010498,GO:0016020,GO:0019538,GO:0019941,GO:0030163,GO:0032991,GO:0043161,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043632,GO:0044237,GO:0044238,GO:0044248,GO:0044257,GO:0044260,GO:0044265,GO:0044267,GO:0044424,GO:0044444,GO:0044464,GO:0051603,GO:0071704,GO:0071944,GO:1901564,GO:1901565,GO:1901575,GO:1902494,GO:1905368,GO:1905369
EggNOG free text desc.26S proteasome non-ATPase regulatory subunit 6. Source PGD
EggNOG OGs1MCW3@121069,COG5187@1,KOG0687@2759
EC1.2.1.12
COG Functional cat.O
Best tax levelPythiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01001,ko03051,ko04131,ko04147
Exons1
Model size456
Cds size456
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816843.5344324-CDS-M-pyrifera_M_contig93081:302..7581622816843.5344324-CDS-M-pyrifera_M_contig93081:302..758Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93081 303..758 -
1692278211.3865094-CDS-M-pyrifera_M_contig93081:302..7581692278211.3865094-CDS-M-pyrifera_M_contig93081:302..758Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig93081 303..758 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig93081.21511.1prot_M-pyrifera_M_contig93081.21511.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig93081 303..758 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig93081.21511.1

>prot_M-pyrifera_M_contig93081.21511.1 ID=prot_M-pyrifera_M_contig93081.21511.1|Name=mRNA_M-pyrifera_M_contig93081.21511.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=152bp
LDSPDVRAVLAKEEMADLKALLHGFYECRYADFFAALIRIAPVVTRDRYL
SQHVPFLVRELRVRAYRQFLDSYRSVTLDGMAEQFGVTTDFVDKELSRFV
AAKRVHAQIDLIRGIVETTRPEDKDKRFADVIRKGDAVLNRLQRLARTLH
V*
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mRNA from alignment at M-pyrifera_M_contig93081:303..758-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig93081.21511.1 ID=mRNA_M-pyrifera_M_contig93081.21511.1|Name=mRNA_M-pyrifera_M_contig93081.21511.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=456bp|location=Sequence derived from alignment at M-pyrifera_M_contig93081:303..758- (Macrocystis pyrifera P11B4 male)
CTAGACTCTCCAGACGTGCGAGCCGTGCTGGCAAAAGAGGAGATGGCCGA
CCTCAAGGCGCTGCTCCACGGGTTTTACGAATGCCGCTACGCCGATTTTT
TCGCAGCGCTCATCCGCATCGCCCCAGTAGTCACCAGAGACCGCTACCTC
AGCCAACACGTGCCGTTCCTCGTCAGGGAGCTGCGCGTCAGAGCGTACCG
CCAGTTCCTCGACTCGTACCGCTCCGTCACCCTCGACGGCATGGCAGAGC
AGTTTGGAGTCACCACCGACTTTGTCGACAAGGAGCTCTCCCGCTTCGTC
GCGGCCAAGCGCGTGCACGCCCAGATCGACCTCATCCGCGGCATCGTCGA
AACCACCAGGCCAGAGGACAAGGACAAGCGATTCGCCGATGTCATCCGAA
AAGGAGACGCTGTCCTCAACAGGCTACAGAGACTAGCGAGAACACTCCAC
GTCTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig93081:303..758-

>mRNA_M-pyrifera_M_contig93081.21511.1 ID=mRNA_M-pyrifera_M_contig93081.21511.1|Name=mRNA_M-pyrifera_M_contig93081.21511.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=912bp|location=Sequence derived from alignment at M-pyrifera_M_contig93081:303..758- (Macrocystis pyrifera P11B4 male)
CTAGACTCTCCAGACGTGCGAGCCGTGCTGGCAAAAGAGGAGATGGCCGA
CCTCAAGGCGCTGCTCCACGGGTTTTACGAATGCCGCTACGCCGATTTTT
TCGCAGCGCTCATCCGCATCGCCCCAGTAGTCACCAGAGACCGCTACCTC
AGCCAACACGTGCCGTTCCTCGTCAGGGAGCTGCGCGTCAGAGCGTACCG
CCAGTTCCTCGACTCGTACCGCTCCGTCACCCTCGACGGCATGGCAGAGC
AGTTTGGAGTCACCACCGACTTTGTCGACAAGGAGCTCTCCCGCTTCGTC
GCGGCCAAGCGCGTGCACGCCCAGATCGACCTCATCCGCGGCATCGTCGA
AACCACCAGGCCAGAGGACAAGGACAAGCGATTCGCCGATGTCATCCGAA
AAGGAGACGCTGTCCTCAACAGGCTACAGAGACTAGCGAGAACACTCCAC
GTCTAGCTAGACTCTCCAGACGTGCGAGCCGTGCTGGCAAAAGAGGAGAT
GGCCGACCTCAAGGCGCTGCTCCACGGGTTTTACGAATGCCGCTACGCCG
ATTTTTTCGCAGCGCTCATCCGCATCGCCCCAGTAGTCACCAGAGACCGC
TACCTCAGCCAACACGTGCCGTTCCTCGTCAGGGAGCTGCGCGTCAGAGC
GTACCGCCAGTTCCTCGACTCGTACCGCTCCGTCACCCTCGACGGCATGG
CAGAGCAGTTTGGAGTCACCACCGACTTTGTCGACAAGGAGCTCTCCCGC
TTCGTCGCGGCCAAGCGCGTGCACGCCCAGATCGACCTCATCCGCGGCAT
CGTCGAAACCACCAGGCCAGAGGACAAGGACAAGCGATTCGCCGATGTCA
TCCGAAAAGGAGACGCTGTCCTCAACAGGCTACAGAGACTAGCGAGAACA
CTCCACGTCTAG
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