mRNA_M-pyrifera_M_contig92539.21402.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig92539.21402.1
Unique NamemRNA_M-pyrifera_M_contig92539.21402.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig92539.21402.1 vs. uniprot
Match: UPI0006451038 (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI0006451038)

HSP 1 Score: 118 bits (296), Expect = 6.090e-28
Identity = 64/165 (38.79%), Postives = 96/165 (58.18%), Query Frame = 1
Query:    1 YRKLFSDYCNNHANAVHTVENLMRKSATFRQLIEEQQAHPRCNGMPFTSFLIKPVQRICKYPLFLRELVKHTEESSAEYNKLVAVLENFNSVVELINETKREADNMAAITRLQRKL-GVKALELLQPGRVFVREGFLQETIKGE---SAMLLYCLFNDILLRAEK 483
            + K++S YC+NH N+   +   ++K A F+Q +EE+QA P C  +   S LIKP+QR+CKYPL LREL+K++++S  +   L        +VV  INE KR AD    + ++  +L   +  ELL P R F+RE  ++E        S    Y LFNDI++R +K
Sbjct:  125 FLKVYSTYCSNHQNSTQHLAACIKKHAHFKQYLEEKQALPECRMIHLESMLIKPIQRLCKYPLLLRELIKNSDKSHPDIPSLNDAYAKIEAVVASINEDKRRADAQQKMFQIHEQLESTEKFELLSPTRYFIREDTIKELTDERDKISGKCTYYLFNDIIMRTKK 289          
BLAST of mRNA_M-pyrifera_M_contig92539.21402.1 vs. uniprot
Match: L8GZK0_ACACA (RhoGEF domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GZK0_ACACA)

HSP 1 Score: 114 bits (284), Expect = 2.610e-26
Identity = 63/165 (38.18%), Postives = 92/165 (55.76%), Query Frame = 1
Query:    1 YRKLFSDYCNNHANAVHTVENLMRKSATFRQLIEEQQAHPRCNGMPFTSFLIKPVQRICKYPLFLRELVKHTEESSAEYNKLVAVLENFNSVVELINETKREADNMAAITRLQRKLGVK---ALELLQPGRVFVREGFLQETIK--GESAMLLYCLFNDILLRAE 480
            Y K++S YC+N A A   V N  + +  FR   E+   +P  NG+    +LIKPVQR+CKYPL +REL+KHT+E+  +Y  L        +VV  +NE K+  ++   I R+  +L       ++L+ PGR  V+EG L E  K   ES    + LF D+L+ AE
Sbjct:  499 YLKMYSQYCSNQAAAREAVVNASKTNPRFRAFQEKMAVNPELNGLTLRDYLIKPVQRLCKYPLLMRELIKHTDEAHVDYPHLQEAFGKIEAVVTSVNEKKQADEDREVIARIISRLSNTEKFGVQLMVPGRRLVQEGKLMEVAKDRSESFRRRFFLFTDLLILAE 663          
BLAST of mRNA_M-pyrifera_M_contig92539.21402.1 vs. uniprot
Match: A0A151Z5E8_9MYCE (Pleckstrin (PH) domain-containing protein n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A151Z5E8_9MYCE)

HSP 1 Score: 113 bits (282), Expect = 5.000e-26
Identity = 59/165 (35.76%), Postives = 95/165 (57.58%), Query Frame = 1
Query:    1 YRKLFSDYCNNHANAVHTVENLMRKSATFRQLIEEQQAHPRCNGMPFTSFLIKPVQRICKYPLFLRELVKHTEESSAEYNKLVAVLENFNSVVELINETKREADNMAAITRLQRKLGVKA-LELLQPGRVFVREGFLQETIKGE---SAMLLYCLFNDILLRAEK 483
            Y K++S YC N   +   + +  +K   F+Q ++E+ A P C   P  SFLIKP+QR+CKYPL LREL+K++ E  ++   L        +VV  +NE+KR+A+ +  + ++  KL  +   + L P R  +RE  L+E  + +   S  + Y LFNDI++R +K
Sbjct:  331 YLKIYSSYCANQKTSSDHITHCSKKFPAFKQFLDEKHASPECRQCPLDSFLIKPIQRLCKYPLLLRELIKNSNEKHSDIVPLEEAYTKIQTVVLSVNESKRKAEVLQKMYKIHEKLETQEKFDFLTPTRYLIREDTLKELTEEKDKVSGKMHYYLFNDIIMRTKK 495          
BLAST of mRNA_M-pyrifera_M_contig92539.21402.1 vs. uniprot
Match: A0A7S4P8Z4_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4P8Z4_9EUKA)

HSP 1 Score: 105 bits (263), Expect = 1.120e-25
Identity = 49/105 (46.67%), Postives = 69/105 (65.71%), Query Frame = 1
Query:    1 YRKLFSDYCNNHANAVHTVENLMRKSATFRQLIEEQQAHPRCNGMPFTSFLIKPVQRICKYPLFLRELVKHTEESSAEYNKLVAVLENFNSVVELINETKREADN 315
            Y K++S YC N   A  TV+ L +K+ TF   +E+ Q  P+C G+ F SFLIKPVQR+CKYPLFLREL+K T +   E   L     + N+ V++INE K++A++
Sbjct:   84 YLKMYSSYCINQDKARETVQELQKKNPTFSTFLEKAQTDPKCRGLLFGSFLIKPVQRLCKYPLFLRELIKFTNDDLKEKEALETAFNDINASVQIINEKKKQAES 188          
BLAST of mRNA_M-pyrifera_M_contig92539.21402.1 vs. uniprot
Match: L8GDW8_ACACA (RhoGEF domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8GDW8_ACACA)

HSP 1 Score: 111 bits (278), Expect = 1.810e-25
Identity = 63/189 (33.33%), Postives = 98/189 (51.85%), Query Frame = 1
Query:    1 YRKLFSDYCNNHANAVHTVENLMRKSATFRQLIEEQ-------------------------QAHPRCNGMPFTSFLIKPVQRICKYPLFLRELVKHTEESSAEYNKLVAVLENFNSVVELINETKREADNMAAITRLQRKLG-----VKALELLQPGRVFVREGFLQETIKGESAMLLYCLFNDILLRA 477
            Y K+++ YC+N   A+  +ENL + +  F   +E Q                         + + RC+     SF+IKP+QRICKYPL LRE+V+HT +   +Y  L+A +     VV  +NE KR+ +N+  +  +Q  +      V  L+L++PGR FV+EG L +   G+     Y LFN+IL+ A
Sbjct:  100 YLKMYTQYCSNQGRALAEIENLKKTNPQFSTFLEAQPPRSPPTLSPFPKLIADPLDLAVECKKNERCHRQDIGSFIIKPIQRICKYPLLLREIVQHTPKDHFDYENLLAAVAKMTEVVTDVNENKRKVENVQKLLEIQNAIVSDTGVVGTLKLVEPGRKFVQEGMLVDLRAGKEMERKYFLFNNILILA 288          
BLAST of mRNA_M-pyrifera_M_contig92539.21402.1 vs. uniprot
Match: A0A0D2WV52_CAPO3 (Uncharacterized protein n=1 Tax=Capsaspora owczarzaki (strain ATCC 30864) TaxID=595528 RepID=A0A0D2WV52_CAPO3)

HSP 1 Score: 111 bits (277), Expect = 2.490e-25
Identity = 65/161 (40.37%), Postives = 95/161 (59.01%), Query Frame = 1
Query:    7 KLFSDYCNNHANAVHTVENLMRKSATFRQLIEEQQAHPRCNGMPFTSFLIKPVQRICKYPLFLRELVKHTEESSAEYNKLVAVLENFNSVVELINETKREADNMAAITRLQRKLGVK---ALELLQPGRVFVREGFLQET---IKGESAMLLYCLFNDILL 471
            K+++ YC N+ +A+  +E  + KS +F+  ++   A   C G   +SFLIKPVQRICKYPL LRE++KHTE S  +Y+K+ A L    +VV ++NE  R A N A +  LQ+++  K   +LEL+ P R  +R+    E     K E   +   LFND LL
Sbjct:  560 KVYTLYCANYEHAIVILEKEL-KSKSFKTFVDNASALEECKGQNISSFLIKPVQRICKYPLLLREILKHTEPSHKDYSKVQAALAKIETVVTVVNEAGRAASNSAKMLDLQQRIKTKDGESLELVAPSRRLLRQALFSEIGPDRKSEEREIF--LFNDFLL 717          
BLAST of mRNA_M-pyrifera_M_contig92539.21402.1 vs. uniprot
Match: L8HIV2_ACACA (RhoGEF domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8HIV2_ACACA)

HSP 1 Score: 108 bits (271), Expect = 1.530e-24
Identity = 59/163 (36.20%), Postives = 100/163 (61.35%), Query Frame = 1
Query:    1 YRKLFSDYCNNHANAVHTVENLMRKSATFRQLIEEQQAHPRCNGMPFTSFLIKPVQRICKYPLFLRELVKHTEESSAEYNKLVAVLENFNSVVELINETKREADNMAAITRLQRKLG-VKAL--ELLQPGRVFVREGFLQE-TIKGESAMLLYCLFNDILLRA 477
            Y K+++ YC N  N + T++ L +K+  F + ++E   +P    + F +FLIKP+QRICKYPL LREL+K+T E   ++ +  A LE    VVE +N+ KR+++N+  I  +Q ++  +  L   L++PGR F+RE    + + +G++      LF+D+L+ A
Sbjct:  392 YFKMYTQYCANQENCIRTMDLLEKKNKPFAKFLDECAENPATRSLSFNAFLIKPIQRICKYPLLLRELLKYTPEEHPDHAQAKAALEKVQGVVEEVNKKKRDSENLQKILEIQGQISNISELGQSLVKPGRQFLREMTCSKISSQGKTQERHLFLFSDLLVFA 554          
BLAST of mRNA_M-pyrifera_M_contig92539.21402.1 vs. uniprot
Match: UPI00064492ED (hypothetical protein n=1 Tax=Acytostelium subglobosum LB1 TaxID=1410327 RepID=UPI00064492ED)

HSP 1 Score: 107 bits (268), Expect = 3.830e-24
Identity = 58/165 (35.15%), Postives = 93/165 (56.36%), Query Frame = 1
Query:    1 YRKLFSDYCNNHANAVHTVENLMRKSATFRQLIEEQQAHPRCNGMPFTSFLIKPVQRICKYPLFLRELVKHTEESSAEYNKLVAVLENFNSVVELINETKREADNMAAITRLQRKLGV-KALELLQPGRVFVREGFLQETIKGE---SAMLLYCLFNDILLRAEK 483
            Y K++S YC N   +   ++  ++KS  F+Q ++E+QA P C      SFLIKP+QR+CKYPL LREL+K+++ +  +   L    +     V  +NE KR+A+    + ++  KL   +  + L P R  +RE  L+E  + +   S    Y LFNDI++R +K
Sbjct:  417 YLKIYSSYCANQQVSHDHLQQCIKKSPVFKQFLDEKQAMPECRQNHLDSFLIKPIQRLCKYPLLLRELIKNSDPAHPDIPSLEEAYDKIQETVITVNENKRKAEVQHKMYKIHEKLETTEKFDFLTPTRYLIREDTLKELTEEKDKISGKSHYYLFNDIIMRTKK 581          
BLAST of mRNA_M-pyrifera_M_contig92539.21402.1 vs. uniprot
Match: A0A8J4PLF0_9MYCE (Uncharacterized protein n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4PLF0_9MYCE)

HSP 1 Score: 107 bits (267), Expect = 5.180e-24
Identity = 60/165 (36.36%), Postives = 94/165 (56.97%), Query Frame = 1
Query:    1 YRKLFSDYCNNHANAVHTVENLMRKSATFRQLIEEQQAHPRCNGMPFTSFLIKPVQRICKYPLFLRELVKHTEESSAEYNKLVAVLENFNSVVELINETKREADNMAAITRLQRKLGVK-ALELLQPGRVFVREGFLQETIKGE---SAMLLYCLFNDILLRAEK 483
            + K++S YC N   +   +    +K   F+Q I+E+QA P C      S LIKPVQR+CKYPL +REL+K++ E+  + + L    E+  +VV  +NE KR+A+N   + ++  KL      + L P R  +RE  L+E  + +   S  + Y LFNDI++R +K
Sbjct:  392 FLKMYSSYCANQKISSDHIALCSKKIPAFKQFIDEKQALPECRQCNLDSLLIKPVQRLCKYPLLIRELIKNSIENHPDNSYLEKAYESIQTVVLSVNENKRKAENDQKMYKIHEKLETSFEFDFLTPTRYLIREDTLRELTEEKDKVSGKMHYYLFNDIMMRTKK 556          
BLAST of mRNA_M-pyrifera_M_contig92539.21402.1 vs. uniprot
Match: A0A7S4KG25_9EUKA (Hypothetical protein n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4KG25_9EUKA)

HSP 1 Score: 104 bits (260), Expect = 7.770e-24
Identity = 60/159 (37.74%), Postives = 86/159 (54.09%), Query Frame = 1
Query:    7 KLFSDYCNNHANAVHTVENLMRKSATFRQLIEEQQAHPRCNGMPFTSFLIKPVQRICKYPLFLRELVKHTEESSAEYNKLVAVLENFNSVVELINETKREADNMAAITRLQRKLGVKALELLQPGRVFVREG--FLQETIKGESAMLLYCLFNDILLRA 477
            K++  YC N   A+ T E LM K+    +  E Q+   RC G+PF S+ IKPVQR+CKYPL LREL+ +T E   ++  +    +    VVE +N  K  A+    +  +Q K+     EL+ P RVFV EG   L+  I G+       LFND+++ A
Sbjct:  115 KMYVQYCVNMKEAIQTQEQLM-KTKKVAKWFETQEQDERCKGLPFGSWAIKPVQRLCKYPLLLRELLLYTPEGHVDHESIKIAKDKIGEVVEAVNTGKGTAEKKNKVIEIQNKMDGLDQELVAPSRVFVMEGDIVLRHQIAGKGENRHLFLFNDLVVIA 272          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig92539.21402.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI00064510386.090e-2838.79hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
L8GZK0_ACACA2.610e-2638.18RhoGEF domain containing protein n=1 Tax=Acanthamo... [more]
A0A151Z5E8_9MYCE5.000e-2635.76Pleckstrin (PH) domain-containing protein n=1 Tax=... [more]
A0A7S4P8Z4_9EUKA1.120e-2546.67Hypothetical protein (Fragment) n=1 Tax=Paramoeba ... [more]
L8GDW8_ACACA1.810e-2533.33RhoGEF domain containing protein n=1 Tax=Acanthamo... [more]
A0A0D2WV52_CAPO32.490e-2540.37Uncharacterized protein n=1 Tax=Capsaspora owczarz... [more]
L8HIV2_ACACA1.530e-2436.20RhoGEF domain containing protein n=1 Tax=Acanthamo... [more]
UPI00064492ED3.830e-2435.15hypothetical protein n=1 Tax=Acytostelium subglobo... [more]
A0A8J4PLF0_9MYCE5.180e-2436.36Uncharacterized protein n=1 Tax=Polysphondylium vi... [more]
A0A7S4KG25_9EUKA7.770e-2437.74Hypothetical protein n=1 Tax=Paramoeba aestuarina ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig92539contigM-pyrifera_M_contig92539:231..763 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeOpisthokonta
Seed ortholog score112.5
Seed ortholog evalue1.9e-22
Seed eggNOG ortholog192875.XP_004344535.1
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Guanine nucleotide exchange factor for Rho/Rac/Cdc42-like GTPases
EggNOG OGs39QFQ@33154,KOG3519@1,KOG3519@2759,KOG4429@1,KOG4429@2759
COG Functional cat.T
Best tax levelOpisthokonta
Best eggNOG OGNA|NA|NA
Exons2
Model size483
Cds size483
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816838.142691-CDS-M-pyrifera_M_contig92539:230..3321622816838.142691-CDS-M-pyrifera_M_contig92539:230..332Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig92539 231..332 +
1692278208.1763527-CDS-M-pyrifera_M_contig92539:230..3321692278208.1763527-CDS-M-pyrifera_M_contig92539:230..332Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig92539 231..332 +
1622816838.1545146-CDS-M-pyrifera_M_contig92539:382..7631622816838.1545146-CDS-M-pyrifera_M_contig92539:382..763Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig92539 383..763 +
1692278208.1883373-CDS-M-pyrifera_M_contig92539:382..7631692278208.1883373-CDS-M-pyrifera_M_contig92539:382..763Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig92539 383..763 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig92539.21402.1prot_M-pyrifera_M_contig92539.21402.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig92539 231..763 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig92539.21402.1

>prot_M-pyrifera_M_contig92539.21402.1 ID=prot_M-pyrifera_M_contig92539.21402.1|Name=mRNA_M-pyrifera_M_contig92539.21402.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=161bp
YRKLFSDYCNNHANAVHTVENLMRKSATFRQLIEEQQAHPRCNGMPFTSF
LIKPVQRICKYPLFLRELVKHTEESSAEYNKLVAVLENFNSVVELINETK
READNMAAITRLQRKLGVKALELLQPGRVFVREGFLQETIKGESAMLLYC
LFNDILLRAEK
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mRNA from alignment at M-pyrifera_M_contig92539:231..763+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig92539.21402.1 ID=mRNA_M-pyrifera_M_contig92539.21402.1|Name=mRNA_M-pyrifera_M_contig92539.21402.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=533bp|location=Sequence derived from alignment at M-pyrifera_M_contig92539:231..763+ (Macrocystis pyrifera P11B4 male)
TACCGCAAACTCTTTTCCGATTATTGCAACAACCATGCCAACGCAGTACA CACCGTGGAGAACCTCATGCGAAAGTCGGCAACCTTCCGCCAACTGATTG AGGTTCGTTGCTTCACCTCTCACGCTGCTGCCTGATGGCTGACACACAGT AGGAGCAGCAGGCGCATCCACGCTGCAATGGCATGCCGTTTACGAGTTTT CTCATCAAGCCGGTGCAGCGTATCTGTAAATACCCTCTGTTCCTGCGAGA ACTCGTCAAGCACACAGAGGAGAGCAGCGCAGAGTACAACAAACTTGTGG CTGTGCTCGAGAACTTCAACTCTGTGGTGGAGCTGATTAACGAGACGAAG AGGGAAGCAGACAACATGGCTGCCATCACTCGCCTGCAGCGAAAGCTCGG CGTGAAGGCACTGGAACTTCTACAGCCAGGCCGTGTGTTTGTGCGAGAGG GCTTCCTCCAGGAGACCATCAAGGGCGAGTCAGCGATGCTCCTCTACTGC TTATTCAACGACATCCTTCTTCGCGCCGAGAAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig92539:231..763+

>mRNA_M-pyrifera_M_contig92539.21402.1 ID=mRNA_M-pyrifera_M_contig92539.21402.1|Name=mRNA_M-pyrifera_M_contig92539.21402.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=966bp|location=Sequence derived from alignment at M-pyrifera_M_contig92539:231..763+ (Macrocystis pyrifera P11B4 male)
TACCGCAAACTCTTTTCCGATTATTGCAACAACCATGCCAACGCAGTACA
CACCGTGGAGAACCTCATGCGAAAGTCGGCAACCTTCCGCCAACTGATTG
AGTACCGCAAACTCTTTTCCGATTATTGCAACAACCATGCCAACGCAGTA
CACACCGTGGAGAACCTCATGCGAAAGTCGGCAACCTTCCGCCAACTGAT
TGAGGAGCAGCAGGCGCATCCACGCTGCAATGGCATGCCGTTTACGAGTT
TTCTCATCAAGCCGGTGCAGCGTATCTGTAAATACCCTCTGTTCCTGCGA
GAACTCGTCAAGCACACAGAGGAGAGCAGCGCAGAGTACAACAAACTTGT
GGCTGTGCTCGAGAACTTCAACTCTGTGGTGGAGCTGATTAACGAGACGA
AGAGGGAAGCAGACAACATGGCTGCCATCACTCGCCTGCAGCGAAAGCTC
GGCGTGAAGGCACTGGAACTTCTACAGCCAGGCCGTGTGTTTGTGCGAGA
GGGCTTCCTCCAGGAGACCATCAAGGGCGAGTCAGCGATGCTCCTCTACT
GCTTATTCAACGACATCCTTCTTCGCGCCGAGAAGGAGCAGCAGGCGCAT
CCACGCTGCAATGGCATGCCGTTTACGAGTTTTCTCATCAAGCCGGTGCA
GCGTATCTGTAAATACCCTCTGTTCCTGCGAGAACTCGTCAAGCACACAG
AGGAGAGCAGCGCAGAGTACAACAAACTTGTGGCTGTGCTCGAGAACTTC
AACTCTGTGGTGGAGCTGATTAACGAGACGAAGAGGGAAGCAGACAACAT
GGCTGCCATCACTCGCCTGCAGCGAAAGCTCGGCGTGAAGGCACTGGAAC
TTCTACAGCCAGGCCGTGTGTTTGTGCGAGAGGGCTTCCTCCAGGAGACC
ATCAAGGGCGAGTCAGCGATGCTCCTCTACTGCTTATTCAACGACATCCT
TCTTCGCGCCGAGAAG
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