mRNA_M-pyrifera_M_contig92012.21302.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig92012.21302.1
Unique NamemRNA_M-pyrifera_M_contig92012.21302.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig92012.21302.1 vs. uniprot
Match: A0A2D5W5U0_9BACT (N-acetylmuramic acid 6-phosphate etherase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2D5W5U0_9BACT)

HSP 1 Score: 164 bits (414), Expect = 1.110e-46
Identity = 98/175 (56.00%), Postives = 116/175 (66.29%), Query Frame = 1
Query:    1 ARGARAGDVVVGITASGRTPYVHGALAWARARGLTTALLACVPATELAPEHTRVDVVLAVPTGPESLAGSTRLRAGTATKLVLNMLSTLTMAQLGRVHKNLMVDVNTGGNAKLWRRGVGLVAELVGCSADRAEELLASAEGRVKVAVVIGACGLDXXXXXXXXXXXXGVLAAALA 525
            ARG    DVV+ I+A G TPY HGALA ARA G  +  LACVP  E AP+  R D+ L + TGPE L GSTRL+AGTATK+VLN +STLTMA+LG++H N MVDV TG NAKL  RG+ LV EL      RA +LL +A GRVK AVV+G CGL+            G L +ALA
Sbjct:  135 ARGLGPADVVIAISAGGTTPYAHGALAAARAAGAASVFLACVP-FEAAPD--RADLSLRLETGPEILTGSTRLKAGTATKMVLNTISTLTMARLGKIHGNRMVDVKTGANAKLRDRGLRLVCELADVDRSRAGQLLDAAGGRVKTAVVMGRCGLERAAAEERLAAADGFLGSALA 306          
BLAST of mRNA_M-pyrifera_M_contig92012.21302.1 vs. uniprot
Match: A0A523PVP8_9BACT (N-acetylmuramic acid 6-phosphate etherase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A523PVP8_9BACT)

HSP 1 Score: 151 bits (382), Expect = 5.040e-42
Identity = 95/174 (54.60%), Postives = 109/174 (62.64%), Query Frame = 1
Query:    1 ARGARAGDVVVGITASGRTPYVHGALAWARARGLTTALLACVPATELAPEHTRVDVVLAVPTGPESLAGSTRLRAGTATKLVLNMLSTLTMAQLGRVHKNLMVDVNTGGNAKLWRRGVGLVAELVGCSADRAEELLASAEGRVKVAVVIGACGLDXXXXXXXXXXXXGVLAAAL 522
            ARG    DVV GITA G TPYVHGAL  ARARG  T   ACV   E+ P+    DV + V TGPE +AGSTRL+AGTATK+VLNM+STLTM QLG+VH NLMVDVNT G+ KL  RG  LV  +   S D A  LL  A G VK+AV++   GL+            G L  AL
Sbjct:  128 ARGIGPADVVFGITAGGTTPYVHGALEAARARGARTVFFACVARAEV-PDT--ADVSIRVLTGPEVIAGSTRLKAGTATKMVLNMVSTLTMVQLGKVHGNLMVDVNTAGSEKLQDRGERLVQHIAAVSRDEARSLLIRASGSVKLAVLVHLRGLNVDEASARLRAAGGHLRRAL 298          
BLAST of mRNA_M-pyrifera_M_contig92012.21302.1 vs. uniprot
Match: A0A521KP63_9BACT (N-acetylmuramic acid 6-phosphate etherase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A521KP63_9BACT)

HSP 1 Score: 151 bits (381), Expect = 7.850e-42
Identity = 94/167 (56.29%), Postives = 107/167 (64.07%), Query Frame = 1
Query:   22 DVVVGITASGRTPYVHGALAWARARGLTTALLACVPATELAPEHTRVDVVLAVPTGPESLAGSTRLRAGTATKLVLNMLSTLTMAQLGRVHKNLMVDVNTGGNAKLWRRGVGLVAELVGCSADRAEELLASAEGRVKVAVVIGACGLDXXXXXXXXXXXXGVLAAAL 522
            DVV+ I A G TP+ HGAL  A+  G  +  LACVP  ELAP+    D+ + V TGPE LAGSTRL+AGTATKLVLN LSTL MA+LG+VH NLMVDVNT  NAKL  RG+GLV +L G     A ELL  AEG VK AVV+ A   D            GVL  AL
Sbjct:  142 DVVLAIAAGGTTPFAHGALEAAQRAGAASIFLACVP-FELAPDS--ADISIRVVTGPEVLAGSTRLKAGTATKLVLNTLSTLVMARLGKVHGNLMVDVNTQANAKLVERGIGLVMQLAGVERAAASELLRRAEGGVKAAVVMHAQRCDLAAARARLAAVGGVLRRAL 305          
BLAST of mRNA_M-pyrifera_M_contig92012.21302.1 vs. uniprot
Match: A0A2E5X2A8_9BACT (N-acetylmuramic acid 6-phosphate etherase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2E5X2A8_9BACT)

HSP 1 Score: 149 bits (377), Expect = 4.050e-41
Identity = 88/148 (59.46%), Postives = 105/148 (70.95%), Query Frame = 1
Query:    4 RGARAGDVVVGITASGRTPYVHGALAWARARGLTTALLACVPATELAPEHTRVDVVLAVPTGPESLAGSTRLRAGTATKLVLNMLSTLTMAQLGRVHKNLMVDVNTGGNAKLWRRGVGLVAELVGCSADRAEELLASAEGRVKVAVVI 447
            RG  AGDV++ I+A G TPY HGA+A ARA G  +  LAC P  + AP+  R D+ L + TGPE LAGSTRL+AGTATK+VLN L+TL MA+LG+VH N MVDV TG NAKL  RG+ LV EL G    RA ELL +A G VK AVV+
Sbjct:  136 RGLNAGDVLIAISAGGTTPYAHGAVAAARAVGAASVFLACTPFAD-APD--RADLTLRLDTGPEILAGSTRLKAGTATKMVLNTLTTLVMARLGKVHGNRMVDVTTGANAKLHDRGLRLVCELAGVDRARAGELLDAAGGHVKTAVVM 280          
BLAST of mRNA_M-pyrifera_M_contig92012.21302.1 vs. uniprot
Match: A0A7Y4RAD7_9BACT (N-acetylmuramic acid 6-phosphate etherase n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7Y4RAD7_9BACT)

HSP 1 Score: 141 bits (356), Expect = 3.840e-38
Identity = 80/149 (53.69%), Postives = 105/149 (70.47%), Query Frame = 1
Query:   19 GDVVVGITASGRTPYVHGALAWARARGLTTALLACVPATELAPEHTRVDVVLAVPTGPESLAGSTRLRAGTATKLVLNMLSTLTMAQLGRVHKNLMVDVNTGGNAKLWRRGVGLVAELVGCSADRAEELLASAEGRVKVAVVIGACGLD 465
            GDVV+GI   G TP+VHGA+  AR  G  T  LACVPA +++ +H   DV + V TGPE + GSTR++AGTATK+VLNM+STLTM  LG+V++NLMVDVNT   AKL  RGV ++ +++G     A  LL  A+G VK A+V+  CG+D
Sbjct:  134 GDVVIGIATGGTTPFVHGAIQRARECGAGTVFLACVPADQVS-DH--ADVSIRVLTGPEVITGSTRMKAGTATKMVLNMISTLTMVGLGKVYQNLMVDVNTRACAKLVDRGVRVLTDVLGIPRSDASALLERADGNVKSAIVMHWCGVD 279          
BLAST of mRNA_M-pyrifera_M_contig92012.21302.1 vs. uniprot
Match: A0A2E6DAV8_9BACT (N-acetylmuramic acid 6-phosphate etherase n=2 Tax=cellular organisms TaxID=131567 RepID=A0A2E6DAV8_9BACT)

HSP 1 Score: 140 bits (353), Expect = 1.260e-37
Identity = 80/148 (54.05%), Postives = 104/148 (70.27%), Query Frame = 1
Query:   22 DVVVGITASGRTPYVHGALAWARARGLTTALLACVPATELAPEHTRVDVVLAVPTGPESLAGSTRLRAGTATKLVLNMLSTLTMAQLGRVHKNLMVDVNTGGNAKLWRRGVGLVAELVGCSADRAEELLASAEGRVKVAVVIGACGLD 465
            DV++GI+A G TP+VH AL  AR RG TT  +  VPA ++ P+    DV + + TGPE +AGSTRL+AGTATK+ LN LSTL M +LG+VH NLMVDV+T  NAKL  RG  ++ EL+G S + A + L +A GRVKVA V+G   +D
Sbjct:  143 DVLLGISAGGTTPFVHAALEQARKRGATTLFMTSVPADQV-PDP--ADVTIRLLTGPELVAGSTRLKAGTATKMALNALSTLVMVRLGKVHGNLMVDVDTSANAKLVERGTRILMELLGSSREEAGQALQAAHGRVKVAAVMGHLEVD 287          
BLAST of mRNA_M-pyrifera_M_contig92012.21302.1 vs. uniprot
Match: A0A800G0T8_9BACT (N-acetylmuramic acid 6-phosphate etherase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A800G0T8_9BACT)

HSP 1 Score: 140 bits (353), Expect = 1.260e-37
Identity = 78/148 (52.70%), Postives = 104/148 (70.27%), Query Frame = 1
Query:   22 DVVVGITASGRTPYVHGALAWARARGLTTALLACVPATELAPEHTRVDVVLAVPTGPESLAGSTRLRAGTATKLVLNMLSTLTMAQLGRVHKNLMVDVNTGGNAKLWRRGVGLVAELVGCSADRAEELLASAEGRVKVAVVIGACGLD 465
            D+++GI+A G TP+VH AL  A+ RG  T  +  VPA ++ P+H   DV + + TGPE +AGSTRL+AGTATK+ LN LSTL M +LG+VH NLMVDV+T  NAKL  RG  ++ EL+G S + A + L +A GRVKVA V+G   +D
Sbjct:  143 DILLGISAGGTTPFVHAALERAQERGAATLFMTSVPADQV-PDH--ADVTIRLLTGPELVAGSTRLKAGTATKMALNALSTLVMVRLGKVHGNLMVDVDTSANAKLVERGTRILMELLGGSREEAGQALQAAHGRVKVAAVMGRLDID 287          
BLAST of mRNA_M-pyrifera_M_contig92012.21302.1 vs. uniprot
Match: A0A3A4PIZ3_9BACT (N-acetylmuramic acid 6-phosphate etherase n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A3A4PIZ3_9BACT)

HSP 1 Score: 140 bits (352), Expect = 1.660e-37
Identity = 80/144 (55.56%), Postives = 99/144 (68.75%), Query Frame = 1
Query:   22 DVVVGITASGRTPYVHGALAWARARGLTTALLACVPATELAPEHTRVDVVLAVPTGPESLAGSTRLRAGTATKLVLNMLSTLTMAQLGRVHKNLMVDVNTGGNAKLWRRGVGLVAELVGCSADRAEELLASAEGRVKVAVVIGA 453
            DVV GIT  G TPYVHGAL  AR RG  T  LACVP  E+       DV + V TGPE L GSTR++AGTATK+VLNM++T+ M ++G+V+ NLMVDV+T  NAKL  RG+ +V ++ G S   A  LL  AEGRVK A+V+ A
Sbjct:  134 DVVFGITTGGTTPYVHGALMEARRRGAKTVFLACVPEGEVGDS---ADVSIRVLTGPEVLTGSTRMKAGTATKMVLNMVTTIAMTRIGKVYGNLMVDVHTRANAKLVDRGIRIVRDVTGLSRQEAAALLEKAEGRVKSALVMHA 274          
BLAST of mRNA_M-pyrifera_M_contig92012.21302.1 vs. uniprot
Match: A0A7V2H875_9BACT (N-acetylmuramic acid 6-phosphate etherase n=1 Tax=Phycisphaerae bacterium TaxID=2026778 RepID=A0A7V2H875_9BACT)

HSP 1 Score: 136 bits (343), Expect = 3.870e-37
Identity = 83/175 (47.43%), Postives = 105/175 (60.00%), Query Frame = 1
Query:    1 ARGARAGDVVVGITASGRTPYVHGALAWARARGLTTALLACVPATELAPEHTRVDVVLAVPTGPESLAGSTRLRAGTATKLVLNMLSTLTMAQLGRVHKNLMVDVNTGGNAKLWRRGVGLVAELVGCSADRAEELLASAEGRVKVAVVIGACGLDXXXXXXXXXXXXGVLAAALA 525
            AR     DVV+GI   G TP+VHGAL  A  RG  T  L+CV   +  P    VDVV+   TGPE L GSTRL+AGTATKLVLN ++T++M QLG+ ++NLMVD+    N KLW RG  ++A L G   D A +LL  A+G VKVA+V+   G+D            G L  AL+
Sbjct:   45 ARNVGPNDVVMGIATGGTTPFVHGALRRAAERGARTIFLSCV---QPVPNEPPVDVVIRPLTGPEVLTGSTRLKAGTATKLVLNQITTISMVQLGKCYENLMVDLRAT-NQKLWDRGARIIAMLTGLDRDSAMDLLRRAQGHVKVAIVMHKLGIDIDQARRRLDASGGFLRKALS 215          
BLAST of mRNA_M-pyrifera_M_contig92012.21302.1 vs. uniprot
Match: A0A2D6HDD5_9BACT (N-acetylmuramic acid 6-phosphate etherase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2D6HDD5_9BACT)

HSP 1 Score: 137 bits (344), Expect = 2.350e-36
Identity = 93/177 (52.54%), Postives = 110/177 (62.15%), Query Frame = 1
Query:    1 ARGARAGDVVVGITASGRTPYVHGALAWARARGLTTALLACVPATELAPEHTRVDVVLAVPTGPESLAGSTRLRAGTATKLVLNMLSTLTMAQLGRVHKNLMVDVNTGGNAKLWRRGVGLVAELVGCSADRAEEL--LASAEGRVKVAVVIGACGLDXXXXXXXXXXXXGVLAAALA 525
            ARG   GDVV GI+A G TP+V GAL  AR RG  T   ACV + E+ P+  R D  + V TGPE+LAGSTRL+AGTATKLVLN +STL MA LG+V+ N MVDVNT  N KL  RG+ LV EL  C  DRA+    L  A G VK+AV++    LD            G L+ ALA
Sbjct:  131 ARGIGRGDVVFGISAGGTTPFVEGALDHARTRGAATVFFACVSSAEV-PD--RADFSIRVLTGPEALAGSTRLKAGTATKLVLNAVSTLAMAGLGKVYGNRMVDVNTLSNVKLRDRGIRLVCEL--CDLDRAQAARQLDRARGSVKLAVLMERHDLDPGPARERLAAAGGDLSRALA 302          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig92012.21302.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2D5W5U0_9BACT1.110e-4656.00N-acetylmuramic acid 6-phosphate etherase n=1 Tax=... [more]
A0A523PVP8_9BACT5.040e-4254.60N-acetylmuramic acid 6-phosphate etherase n=1 Tax=... [more]
A0A521KP63_9BACT7.850e-4256.29N-acetylmuramic acid 6-phosphate etherase n=1 Tax=... [more]
A0A2E5X2A8_9BACT4.050e-4159.46N-acetylmuramic acid 6-phosphate etherase n=1 Tax=... [more]
A0A7Y4RAD7_9BACT3.840e-3853.69N-acetylmuramic acid 6-phosphate etherase n=1 Tax=... [more]
A0A2E6DAV8_9BACT1.260e-3754.05N-acetylmuramic acid 6-phosphate etherase n=2 Tax=... [more]
A0A800G0T8_9BACT1.260e-3752.70N-acetylmuramic acid 6-phosphate etherase n=1 Tax=... [more]
A0A3A4PIZ3_9BACT1.660e-3755.56N-acetylmuramic acid 6-phosphate etherase n=1 Tax=... [more]
A0A7V2H875_9BACT3.870e-3747.43N-acetylmuramic acid 6-phosphate etherase n=1 Tax=... [more]
A0A2D6HDD5_9BACT2.350e-3652.54N-acetylmuramic acid 6-phosphate etherase n=1 Tax=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig92012contigM-pyrifera_M_contig92012:3..533 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score136.3
Seed ortholog evalue1.3e-29
Seed eggNOG ortholog1396418.BATQ01000049_gene368
Preferred namemurQ
KEGG rclassRC00002,RC00017,RC00397,RC00746
KEGG koko:K07106,ko:K18676
KEGG ReactionR01961,R08555
KEGG Pathwayko00520,ko01100,map00520,map01100
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0006793,GO:0006796,GO:0008150,GO:0008152,GO:0009987,GO:0016301,GO:0016310,GO:0016740,GO:0016772,GO:0016773,GO:0044237,GO:0047931
EggNOG free text desc.Specifically catalyzes the cleavage of the D-lactyl ether substituent of MurNAc 6-phosphate, producing GlcNAc 6- phosphate and D-lactate
EggNOG OGs46SJI@74201,COG2103@1,COG2103@2,COG2971@1,COG2971@2
EC2.7.1.8,4.2.1.126
COG Functional cat.G
Best tax levelVerrucomicrobia
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons1
Model size531
Cds size531
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816833.1887524-CDS-M-pyrifera_M_contig92012:2..5331622816833.1887524-CDS-M-pyrifera_M_contig92012:2..533Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig92012 3..533 +
1692278205.3236396-CDS-M-pyrifera_M_contig92012:2..5331692278205.3236396-CDS-M-pyrifera_M_contig92012:2..533Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig92012 3..533 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig92012.21302.1prot_M-pyrifera_M_contig92012.21302.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig92012 3..533 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig92012.21302.1

>prot_M-pyrifera_M_contig92012.21302.1 ID=prot_M-pyrifera_M_contig92012.21302.1|Name=mRNA_M-pyrifera_M_contig92012.21302.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=177bp
ARGARAGDVVVGITASGRTPYVHGALAWARARGLTTALLACVPATELAPE
HTRVDVVLAVPTGPESLAGSTRLRAGTATKLVLNMLSTLTMAQLGRVHKN
LMVDVNTGGNAKLWRRGVGLVAELVGCSADRAEELLASAEGRVKVAVVIG
ACGLDAEAAEARLAAADGVLAAALAD*
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mRNA from alignment at M-pyrifera_M_contig92012:3..533+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig92012.21302.1 ID=mRNA_M-pyrifera_M_contig92012.21302.1|Name=mRNA_M-pyrifera_M_contig92012.21302.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=531bp|location=Sequence derived from alignment at M-pyrifera_M_contig92012:3..533+ (Macrocystis pyrifera P11B4 male)
GCGCGCGGTGCGCGGGCCGGGGACGTGGTCGTCGGCATCACCGCCAGCGG GCGCACGCCCTACGTCCACGGCGCCCTCGCCTGGGCCCGCGCGCGTGGGC TCACCACCGCGCTGCTCGCGTGCGTGCCCGCCACCGAGCTCGCGCCGGAG CACACCCGCGTCGACGTCGTGCTCGCGGTGCCGACCGGCCCCGAGAGCCT GGCCGGCAGCACGCGCCTGCGCGCCGGCACCGCGACCAAGCTCGTGCTCA ACATGCTCAGCACGCTGACGATGGCCCAGCTCGGGCGCGTGCACAAGAAC CTGATGGTCGACGTGAACACCGGCGGCAACGCCAAGCTCTGGCGCCGCGG CGTGGGCTTGGTCGCCGAGCTCGTCGGCTGCAGTGCGGATCGCGCGGAGG AGCTGCTCGCCAGCGCCGAGGGCCGGGTCAAGGTCGCGGTCGTGATAGGG GCCTGCGGTCTGGATGCCGAGGCCGCCGAGGCGCGCCTCGCCGCGGCGGA CGGGGTGCTCGCCGCCGCGCTCGCCGATTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig92012:3..533+

>mRNA_M-pyrifera_M_contig92012.21302.1 ID=mRNA_M-pyrifera_M_contig92012.21302.1|Name=mRNA_M-pyrifera_M_contig92012.21302.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1062bp|location=Sequence derived from alignment at M-pyrifera_M_contig92012:3..533+ (Macrocystis pyrifera P11B4 male)
GCGCGCGGTGCGCGGGCCGGGGACGTGGTCGTCGGCATCACCGCCAGCGG
GCGCACGCCCTACGTCCACGGCGCCCTCGCCTGGGCCCGCGCGCGTGGGC
TCACCACCGCGCTGCTCGCGTGCGTGCCCGCCACCGAGCTCGCGCCGGAG
CACACCCGCGTCGACGTCGTGCTCGCGGTGCCGACCGGCCCCGAGAGCCT
GGCCGGCAGCACGCGCCTGCGCGCCGGCACCGCGACCAAGCTCGTGCTCA
ACATGCTCAGCACGCTGACGATGGCCCAGCTCGGGCGCGTGCACAAGAAC
CTGATGGTCGACGTGAACACCGGCGGCAACGCCAAGCTCTGGCGCCGCGG
CGTGGGCTTGGTCGCCGAGCTCGTCGGCTGCAGTGCGGATCGCGCGGAGG
AGCTGCTCGCCAGCGCCGAGGGCCGGGTCAAGGTCGCGGTCGTGATAGGG
GCCTGCGGTCTGGATGCCGAGGCCGCCGAGGCGCGCCTCGCCGCGGCGGA
CGGGGTGCTCGCCGCCGCGCTCGCCGATTAGGCGCGCGGTGCGCGGGCCG
GGGACGTGGTCGTCGGCATCACCGCCAGCGGGCGCACGCCCTACGTCCAC
GGCGCCCTCGCCTGGGCCCGCGCGCGTGGGCTCACCACCGCGCTGCTCGC
GTGCGTGCCCGCCACCGAGCTCGCGCCGGAGCACACCCGCGTCGACGTCG
TGCTCGCGGTGCCGACCGGCCCCGAGAGCCTGGCCGGCAGCACGCGCCTG
CGCGCCGGCACCGCGACCAAGCTCGTGCTCAACATGCTCAGCACGCTGAC
GATGGCCCAGCTCGGGCGCGTGCACAAGAACCTGATGGTCGACGTGAACA
CCGGCGGCAACGCCAAGCTCTGGCGCCGCGGCGTGGGCTTGGTCGCCGAG
CTCGTCGGCTGCAGTGCGGATCGCGCGGAGGAGCTGCTCGCCAGCGCCGA
GGGCCGGGTCAAGGTCGCGGTCGTGATAGGGGCCTGCGGTCTGGATGCCG
AGGCCGCCGAGGCGCGCCTCGCCGCGGCGGACGGGGTGCTCGCCGCCGCG
CTCGCCGATTAG
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