mRNA_M-pyrifera_M_contig91369.21175.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig91369.21175.1
Unique NamemRNA_M-pyrifera_M_contig91369.21175.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig91369.21175.1 vs. uniprot
Match: A0A517VEK6_9PLAN (CRISPR-associated endonuclease Cas9 n=17 Tax=Planctomycetaceae TaxID=126 RepID=A0A517VEK6_9PLAN)

HSP 1 Score: 295 bits (755), Expect = 4.320e-100
Identity = 139/151 (92.05%), Postives = 148/151 (98.01%), Query Frame = 1
Query:    1 ISGLRSEFNERSELEDWILTVNYEIQVPRVVRLLRYDRIPNNTIKFNRRNIFIRDSYRCQYCQKKFNVKQLSLDHVVPRSHGGGMSWENIVSACRRCNTKKGGRTPTQAGMKLLQKPAKPSRNPVLLQQVKQAKYECWKNFVGTKELINCD 453
            IS LR+EFNER+ELEDWIL+VN+EIQVPRVVRLLRYDRIPNNTIKFNRRNIFIRDSYRCQYCQKKF+VKQLSLDHV+PRSHGGGMSWENIVSACRRCNTKKGGRTP+QAGMKL QKPAKPSRNPVLLQQVKQAKYECWKNFVG+KEL+ CD
Sbjct:   66 ISELRAEFNERNELEDWILSVNFEIQVPRVVRLLRYDRIPNNTIKFNRRNIFIRDSYRCQYCQKKFSVKQLSLDHVIPRSHGGGMSWENIVSACRRCNTKKGGRTPSQAGMKLFQKPAKPSRNPVLLQQVKQAKYECWKNFVGSKELLTCD 216          
BLAST of mRNA_M-pyrifera_M_contig91369.21175.1 vs. uniprot
Match: A0A350K5I4_9PLAN (HNH endonuclease (Fragment) n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A350K5I4_9PLAN)

HSP 1 Score: 226 bits (576), Expect = 2.710e-74
Identity = 105/115 (91.30%), Postives = 110/115 (95.65%), Query Frame = 1
Query:  109 DRIPNNTIKFNRRNIFIRDSYRCQYCQKKFNVKQLSLDHVVPRSHGGGMSWENIVSACRRCNTKKGGRTPTQAGMKLLQKPAKPSRNPVLLQQVKQAKYECWKNFVGTKELINCD 453
            DRIPN TIKFNRRNIFIRDSYRCQYCQKKF++KQLSLDHV+PRSHGGGMSWENIVSACRRCNTKKGGRTP+QAGMKL QKPAKPSRNPVLLQQVK AKYE WKNFVGTKEL+ CD
Sbjct:    1 DRIPNTTIKFNRRNIFIRDSYRCQYCQKKFSLKQLSLDHVIPRSHGGGMSWENIVSACRRCNTKKGGRTPSQAGMKLFQKPAKPSRNPVLLQQVKHAKYESWKNFVGTKELLTCD 115          
BLAST of mRNA_M-pyrifera_M_contig91369.21175.1 vs. uniprot
Match: A0A3B1D371_9ZZZZ (HNH endonuclease family protein n=1 Tax=hydrothermal vent metagenome TaxID=652676 RepID=A0A3B1D371_9ZZZZ)

HSP 1 Score: 197 bits (501), Expect = 1.250e-61
Identity = 87/142 (61.27%), Postives = 110/142 (77.46%), Query Frame = 1
Query:    1 ISGLRSEFNERSELEDWILTVNYEIQVPRVVRLLRYDRIPNNTIKFNRRNIFIRDSYRCQYCQKKFNVKQLSLDHVVPRSHGGGMSWENIVSACRRCNTKKGGRTPTQAGMKLLQKPAKPSRNPVLLQQVKQAKYECWKNFV 426
            +S L+ E NER E +DW+L VN++IQVPRV+RLL YDR+P   IKFNRRN+F+RD + CQYC  +F+  +LSLDHV+PRS GG ++WEN+VSAC +CN +KG RTP +AGM L + P KP RNPVL QQ+   KY CWKNFV
Sbjct:   61 VSELKIELNERHEHDDWLLAVNFQIQVPRVIRLLTYDRVPRQAIKFNRRNVFLRDDHHCQYCGNQFHSHRLSLDHVLPRSRGGKLTWENVVSACHKCNVRKGNRTPNEAGMNLSRPPKKPQRNPVLAQQLSIPKYSCWKNFV 202          
BLAST of mRNA_M-pyrifera_M_contig91369.21175.1 vs. uniprot
Match: A0A1I3G0R5_9PLAN (5-methylcytosine-specific restriction endonuclease McrA n=1 Tax=Planctomicrobium piriforme TaxID=1576369 RepID=A0A1I3G0R5_9PLAN)

HSP 1 Score: 195 bits (496), Expect = 1.670e-60
Identity = 87/142 (61.27%), Postives = 113/142 (79.58%), Query Frame = 1
Query:    1 ISGLRSEFNERSELEDWILTVNYEIQVPRVVRLLRYDRIPNNTIKFNRRNIFIRDSYRCQYCQKKFNVKQLSLDHVVPRSHGGGMSWENIVSACRRCNTKKGGRTPTQAGMKLLQKPAKPSRNPVLLQQVKQAKYECWKNFV 426
            +  L+    E+ + EDWI  VN+EIQVPRVVRLL YDR+P NT+KFNRRNIF+RD +RCQYC K+++  +LSLDHV+P+S GG  +WENIV AC +CN  KGGRTP++AGMKLLQKP KP R+P+L +Q+  +KYE W+NF+
Sbjct:   84 LCDLKVAVGEQRDYEDWIRAVNFEIQVPRVVRLLTYDRVPRNTVKFNRRNIFLRDGHRCQYCGKRYSTPRLSLDHVMPKSRGGADTWENIVCACLKCNVDKGGRTPSEAGMKLLQKPIKPKRSPLLSRQLSLSKYESWRNFI 225          
BLAST of mRNA_M-pyrifera_M_contig91369.21175.1 vs. uniprot
Match: A0A5C5VQ43_9PLAN (HNH endonuclease n=1 Tax=Thalassoglobus neptunius TaxID=1938619 RepID=A0A5C5VQ43_9PLAN)

HSP 1 Score: 192 bits (488), Expect = 2.650e-59
Identity = 85/142 (59.86%), Postives = 112/142 (78.87%), Query Frame = 1
Query:    1 ISGLRSEFNERSELEDWILTVNYEIQVPRVVRLLRYDRIPNNTIKFNRRNIFIRDSYRCQYCQKKFNVKQLSLDHVVPRSHGGGMSWENIVSACRRCNTKKGGRTPTQAGMKLLQKPAKPSRNPVLLQQVKQAKYECWKNFV 426
            +S L+S  NE+ E EDWI  VN+EIQVPRV+RL  YDR+P N +KFNRRNIF+RD +RCQYC  +++  +LSLDHV+P+S GG  +WENIV AC +CN  KGGRTP +AGM+LLQ+P KP R+P+L +Q+ Q KYE W++F+
Sbjct:   83 MSELKSALNEKKEHEDWIRAVNFEIQVPRVIRLTTYDRVPRNAVKFNRRNIFLRDGHRCQYCGNRYSSPRLSLDHVIPKSRGGPETWENIVCACLKCNVDKGGRTPQEAGMRLLQQPIKPKRSPLLNRQLNQPKYESWRHFI 224          
BLAST of mRNA_M-pyrifera_M_contig91369.21175.1 vs. uniprot
Match: A0A836RKM6_9PLAN (HNH endonuclease n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A836RKM6_9PLAN)

HSP 1 Score: 188 bits (477), Expect = 7.520e-58
Identity = 85/142 (59.86%), Postives = 109/142 (76.76%), Query Frame = 1
Query:    1 ISGLRSEFNERSELEDWILTVNYEIQVPRVVRLLRYDRIPNNTIKFNRRNIFIRDSYRCQYCQKKFNVKQLSLDHVVPRSHGGGMSWENIVSACRRCNTKKGGRTPTQAGMKLLQKPAKPSRNPVLLQQVKQAKYECWKNFV 426
            IS L++  NER + EDW+  V++EI+VPR +RLL YDR+P N +KFNRRNIF+RD  RCQYC K+F  + LSLDHV+PRS GG  +WENIV AC RCN +KGGRTP +AGMKL++ P KPSR+P+L  ++   KY  WKNF+
Sbjct:   61 ISELKAALNERGQDEDWVQAVDFEIEVPRTIRLLDYDRVPRNVVKFNRRNIFLRDGNRCQYCGKRFASQDLSLDHVIPRSRGGQSTWENIVCACLRCNVRKGGRTPPEAGMKLIRPPKKPSRSPLLTVKLNLRKYASWKNFL 202          
BLAST of mRNA_M-pyrifera_M_contig91369.21175.1 vs. uniprot
Match: A0A517QUU3_9PLAN (HNH endonuclease n=1 Tax=Thalassoglobus polymorphus TaxID=2527994 RepID=A0A517QUU3_9PLAN)

HSP 1 Score: 188 bits (477), Expect = 1.200e-57
Identity = 85/139 (61.15%), Postives = 109/139 (78.42%), Query Frame = 1
Query:   10 LRSEFNERSELEDWILTVNYEIQVPRVVRLLRYDRIPNNTIKFNRRNIFIRDSYRCQYCQKKFNVKQLSLDHVVPRSHGGGMSWENIVSACRRCNTKKGGRTPTQAGMKLLQKPAKPSRNPVLLQQVKQAKYECWKNFV 426
            L+ +  ER + EDWI  VN+EIQVPRV+RL  YDR+P NT+KFNRRNIF+RD +RCQYC  +++  +LSLDHV+P+S GG  +WENIV AC  CN  KGGRTP +AGM LLQKP+KP R+P+L +Q+ Q KYE WK+F+
Sbjct:   85 LKLDLGERKDHEDWIRAVNFEIQVPRVIRLSTYDRLPKNTVKFNRRNIFLRDGHRCQYCGNRYSSPRLSLDHVMPKSRGGPDTWENIVCACLDCNVSKGGRTPQEAGMPLLQKPSKPKRSPLLNRQLMQPKYESWKHFI 223          
BLAST of mRNA_M-pyrifera_M_contig91369.21175.1 vs. uniprot
Match: A0A3L7PIJ7_9BACT (HNH endonuclease n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3L7PIJ7_9BACT)

HSP 1 Score: 187 bits (476), Expect = 1.860e-57
Identity = 82/141 (58.16%), Postives = 110/141 (78.01%), Query Frame = 1
Query:    4 SGLRSEFNERSELEDWILTVNYEIQVPRVVRLLRYDRIPNNTIKFNRRNIFIRDSYRCQYCQKKFNVKQLSLDHVVPRSHGGGMSWENIVSACRRCNTKKGGRTPTQAGMKLLQKPAKPSRNPVLLQQVKQAKYECWKNFV 426
            S L+ E   R   EDWI  VN+EI+VPRV+RLL+YDR+P NT+KFNRRN+F+RD +RCQYC ++F+V++LSLDHV+PRS GG  +WEN+V AC  CN +KGGRTP +AGM+LL +P +P RNP++ +Q+   KY  WK F+
Sbjct:   83 SRLKEELGLRDPHEDWIQAVNFEIEVPRVIRLLKYDRVPRNTVKFNRRNVFLRDEHRCQYCARRFSVQRLSLDHVMPRSKGGPDTWENVVCACLTCNVRKGGRTPHEAGMRLLSEPVRPKRNPLVSRQLMLQKYSAWKAFI 223          
BLAST of mRNA_M-pyrifera_M_contig91369.21175.1 vs. uniprot
Match: A0A3B8PQ53_9PLAN (HNH endonuclease n=2 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A3B8PQ53_9PLAN)

HSP 1 Score: 187 bits (475), Expect = 2.190e-57
Identity = 84/146 (57.53%), Postives = 110/146 (75.34%), Query Frame = 1
Query:    1 ISGLRSEFNERSELEDWILTVNYEIQVPRVVRLLRYDRIPNNTIKFNRRNIFIRDSYRCQYCQKKFNVKQLSLDHVVPRSHGGGMSWENIVSACRRCNTKKGGRTPTQAGMKLLQKPAKPSRNPVLLQQVKQAKYECWKNFVGTKE 438
            +S L+  F ER   EDWI  VN+ I+VPRV+RLLRYDR+P + +KFNR NIF+RD  RCQYC+++F+  +LSLDHVVPRS GG  +W+NIVSAC +CN  KGGRTP +AGM+L+ +P KP+R+P+L + V   KY  WK FVG  +
Sbjct:   60 VSELKLAFGERGADEDWINAVNFSIEVPRVIRLLRYDRVPQHVVKFNRHNIFLRDHNRCQYCRRRFSSAELSLDHVVPRSRGGRTTWDNIVSACLKCNVVKGGRTPREAGMELVSRPRKPTRSPLLAETVNSQKYATWKTFVGESD 205          
BLAST of mRNA_M-pyrifera_M_contig91369.21175.1 vs. uniprot
Match: A0A3M1V3N7_9BACT (HNH endonuclease n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3M1V3N7_9BACT)

HSP 1 Score: 186 bits (471), Expect = 4.770e-57
Identity = 79/142 (55.63%), Postives = 110/142 (77.46%), Query Frame = 1
Query:    1 ISGLRSEFNERSELEDWILTVNYEIQVPRVVRLLRYDRIPNNTIKFNRRNIFIRDSYRCQYCQKKFNVKQLSLDHVVPRSHGGGMSWENIVSACRRCNTKKGGRTPTQAGMKLLQKPAKPSRNPVLLQQVKQAKYECWKNFV 426
            +S LR E NE SE +DWI++VN+ +Q PR++RLL +DR+P + +KFNRRNIF+RD +RCQYC ++F+ + LSLDHVVPRS GG  +W+NIV AC +CN +KG RTP +AGM+L + P +P RNP++  ++   KYECW+ FV
Sbjct:   63 LSQLRRELNEYSESDDWIVSVNFYVQAPRIIRLLEFDRVPEDGVKFNRRNIFLRDEHRCQYCGRQFSSRHLSLDHVVPRSRGGRATWDNIVCACLKCNVRKGSRTPQEAGMRLQRPPRRPRRNPMITHKLNSRKYECWRTFV 204          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig91369.21175.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A517VEK6_9PLAN4.320e-10092.05CRISPR-associated endonuclease Cas9 n=17 Tax=Planc... [more]
A0A350K5I4_9PLAN2.710e-7491.30HNH endonuclease (Fragment) n=1 Tax=Planctomycetac... [more]
A0A3B1D371_9ZZZZ1.250e-6161.27HNH endonuclease family protein n=1 Tax=hydrotherm... [more]
A0A1I3G0R5_9PLAN1.670e-6061.275-methylcytosine-specific restriction endonuclease... [more]
A0A5C5VQ43_9PLAN2.650e-5959.86HNH endonuclease n=1 Tax=Thalassoglobus neptunius ... [more]
A0A836RKM6_9PLAN7.520e-5859.86HNH endonuclease n=1 Tax=Planctomycetaceae bacteri... [more]
A0A517QUU3_9PLAN1.200e-5761.15HNH endonuclease n=1 Tax=Thalassoglobus polymorphu... [more]
A0A3L7PIJ7_9BACT1.860e-5758.16HNH endonuclease n=1 Tax=Planctomycetes bacterium ... [more]
A0A3B8PQ53_9PLAN2.190e-5757.53HNH endonuclease n=2 Tax=Planctomycetaceae bacteri... [more]
A0A3M1V3N7_9BACT4.770e-5755.63HNH endonuclease n=1 Tax=Planctomycetes bacterium ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig91369contigM-pyrifera_M_contig91369:2..457 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score293.9
Seed ortholog evalue4.3e-77
Seed eggNOG ortholog344747.PM8797T_07182
Hectar predicted targeting categoryother localisation
EggNOG free text desc.PFAM HNH endonuclease
EggNOG OGs2IXXW@203682,COG1403@1,COG1403@2
COG Functional cat.L
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
Exons1
Model size456
Cds size456
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816826.3585343-CDS-M-pyrifera_M_contig91369:1..4571622816826.3585343-CDS-M-pyrifera_M_contig91369:1..457Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91369 2..457 +
1692278201.183088-CDS-M-pyrifera_M_contig91369:1..4571692278201.183088-CDS-M-pyrifera_M_contig91369:1..457Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91369 2..457 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig91369.21175.1prot_M-pyrifera_M_contig91369.21175.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig91369 2..457 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig91369.21175.1

>prot_M-pyrifera_M_contig91369.21175.1 ID=prot_M-pyrifera_M_contig91369.21175.1|Name=mRNA_M-pyrifera_M_contig91369.21175.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=152bp
ISGLRSEFNERSELEDWILTVNYEIQVPRVVRLLRYDRIPNNTIKFNRRN
IFIRDSYRCQYCQKKFNVKQLSLDHVVPRSHGGGMSWENIVSACRRCNTK
KGGRTPTQAGMKLLQKPAKPSRNPVLLQQVKQAKYECWKNFVGTKELINC
D*
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mRNA from alignment at M-pyrifera_M_contig91369:2..457+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig91369.21175.1 ID=mRNA_M-pyrifera_M_contig91369.21175.1|Name=mRNA_M-pyrifera_M_contig91369.21175.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=456bp|location=Sequence derived from alignment at M-pyrifera_M_contig91369:2..457+ (Macrocystis pyrifera P11B4 male)
ATCAGTGGATTACGTTCGGAGTTCAACGAACGCAGCGAACTCGAAGACTG GATCCTCACCGTCAATTACGAAATCCAGGTTCCCCGCGTCGTCCGGTTAC TCCGCTATGACCGCATCCCCAACAATACCATCAAGTTCAATCGACGTAAC ATTTTCATCCGCGACAGTTATCGCTGTCAGTACTGCCAGAAGAAATTCAA CGTCAAACAGCTCAGCCTGGATCACGTCGTCCCCCGCTCGCATGGCGGCG GCATGAGCTGGGAAAACATTGTCAGCGCCTGTCGTCGCTGTAACACCAAA AAAGGGGGCCGCACCCCGACCCAAGCCGGTATGAAACTTCTTCAGAAACC GGCCAAGCCCAGCCGCAACCCGGTACTCCTGCAGCAGGTGAAACAGGCGA AATATGAATGCTGGAAAAATTTTGTCGGCACCAAAGAACTCATCAACTGT GACTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig91369:2..457+

>mRNA_M-pyrifera_M_contig91369.21175.1 ID=mRNA_M-pyrifera_M_contig91369.21175.1|Name=mRNA_M-pyrifera_M_contig91369.21175.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=912bp|location=Sequence derived from alignment at M-pyrifera_M_contig91369:2..457+ (Macrocystis pyrifera P11B4 male)
ATCAGTGGATTACGTTCGGAGTTCAACGAACGCAGCGAACTCGAAGACTG
GATCCTCACCGTCAATTACGAAATCCAGGTTCCCCGCGTCGTCCGGTTAC
TCCGCTATGACCGCATCCCCAACAATACCATCAAGTTCAATCGACGTAAC
ATTTTCATCCGCGACAGTTATCGCTGTCAGTACTGCCAGAAGAAATTCAA
CGTCAAACAGCTCAGCCTGGATCACGTCGTCCCCCGCTCGCATGGCGGCG
GCATGAGCTGGGAAAACATTGTCAGCGCCTGTCGTCGCTGTAACACCAAA
AAAGGGGGCCGCACCCCGACCCAAGCCGGTATGAAACTTCTTCAGAAACC
GGCCAAGCCCAGCCGCAACCCGGTACTCCTGCAGCAGGTGAAACAGGCGA
AATATGAATGCTGGAAAAATTTTGTCGGCACCAAAGAACTCATCAACTGT
GACTGAATCAGTGGATTACGTTCGGAGTTCAACGAACGCAGCGAACTCGA
AGACTGGATCCTCACCGTCAATTACGAAATCCAGGTTCCCCGCGTCGTCC
GGTTACTCCGCTATGACCGCATCCCCAACAATACCATCAAGTTCAATCGA
CGTAACATTTTCATCCGCGACAGTTATCGCTGTCAGTACTGCCAGAAGAA
ATTCAACGTCAAACAGCTCAGCCTGGATCACGTCGTCCCCCGCTCGCATG
GCGGCGGCATGAGCTGGGAAAACATTGTCAGCGCCTGTCGTCGCTGTAAC
ACCAAAAAAGGGGGCCGCACCCCGACCCAAGCCGGTATGAAACTTCTTCA
GAAACCGGCCAAGCCCAGCCGCAACCCGGTACTCCTGCAGCAGGTGAAAC
AGGCGAAATATGAATGCTGGAAAAATTTTGTCGGCACCAAAGAACTCATC
AACTGTGACTGA
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