mRNA_M-pyrifera_M_contig91295.21160.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig91295.21160.1
Unique NamemRNA_M-pyrifera_M_contig91295.21160.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig91295.21160.1 vs. uniprot
Match: A0A2D9CLI0_9BACT (tRNA pseudouridine synthase A n=1 Tax=Phycisphaerae bacterium TaxID=2026778 RepID=A0A2D9CLI0_9BACT)

HSP 1 Score: 120 bits (301), Expect = 1.240e-31
Identity = 61/83 (73.49%), Postives = 69/83 (83.13%), Query Frame = 1
Query:    1 RTVFSCSV-NAHPTEQGRVEIRISGSGFLYNMVRIVAGALMEVGRGKIDPDTIPDIIASCDRTQAGPTLPPQGLRLEWIRYEE 246
            RT++SC V N  P     V IR+SGSGFLYNMVRI+AG LMEVGRGKIDP+TIP+IIAS DR +AG TLPPQGLRLEWIRY+E
Sbjct:  196 RTIYSCVVENPKPDT---VVIRVSGSGFLYNMVRIIAGTLMEVGRGKIDPETIPEIIASGDRKRAGTTLPPQGLRLEWIRYDE 275          
BLAST of mRNA_M-pyrifera_M_contig91295.21160.1 vs. uniprot
Match: A0A7C5WJG6_9BACT (tRNA pseudouridine synthase A n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7C5WJG6_9BACT)

HSP 1 Score: 119 bits (297), Expect = 7.970e-31
Identity = 59/90 (65.56%), Postives = 71/90 (78.89%), Query Frame = 1
Query:    1 RTVFSCSVNA--------HPTEQGRVEIRISGSGFLYNMVRIVAGALMEVGRGKIDPDTIPDIIASCDRTQAGPTLPPQGLRLEWIRYEE 246
            RT+F+C V A        +P E  R+ IR+SGSGFLYNMVRI+AG L+E GRG+IDP  IP+I+AS DRT+AGPTLPPQGLRLEWIRYE+
Sbjct:  211 RTIFACDVEAGGGGAARANPDET-RLVIRVSGSGFLYNMVRIIAGTLLEAGRGRIDPAHIPEILASKDRTRAGPTLPPQGLRLEWIRYED 299          
BLAST of mRNA_M-pyrifera_M_contig91295.21160.1 vs. uniprot
Match: A0A3M2BES3_9BACT (tRNA pseudouridine synthase A n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3M2BES3_9BACT)

HSP 1 Score: 115 bits (288), Expect = 1.560e-29
Identity = 53/84 (63.10%), Postives = 66/84 (78.57%), Query Frame = 1
Query:    1 RTVFSCSVNAHPTEQG---RVEIRISGSGFLYNMVRIVAGALMEVGRGKIDPDTIPDIIASCDRTQAGPTLPPQGLRLEWIRYE 243
            RTV+ C V   P + G   R+ IR+SG GFLYNMVRI+AG LMEVGRG+++P  I +IIAS DR +AGPTLPP+GLRLEW++YE
Sbjct:  205 RTVYRCDVECEPCDDGHNQRLVIRVSGDGFLYNMVRIIAGTLMEVGRGRMEPTAIGEIIASLDRRRAGPTLPPEGLRLEWVKYE 288          
BLAST of mRNA_M-pyrifera_M_contig91295.21160.1 vs. uniprot
Match: A0A3M8FY44_9BACT (tRNA pseudouridine synthase A n=1 Tax=Phycisphaera sp. TaxID=2030824 RepID=A0A3M8FY44_9BACT)

HSP 1 Score: 112 bits (281), Expect = 1.160e-28
Identity = 56/83 (67.47%), Postives = 61/83 (73.49%), Query Frame = 1
Query:    1 RTVFSCSVNAHPTEQGRVE--IRISGSGFLYNMVRIVAGALMEVGRGKIDPDTIPDIIASCDRTQAGPTLPPQGLRLEWIRYE 243
            RT+F C V       G V   +R+SGSGFLYNMVRI+AG LMEVGRGKI PD I  II S DR  AGPTLPPQGLRLEWIRY+
Sbjct:  189 RTIFGCRVERAQAGHGAVRFVVRVSGSGFLYNMVRIIAGTLMEVGRGKIAPDAIGGIIESKDRRNAGPTLPPQGLRLEWIRYD 271          
BLAST of mRNA_M-pyrifera_M_contig91295.21160.1 vs. uniprot
Match: A0A5M4D386_9CYAN (tRNA pseudouridine synthase A n=1 Tax=Cyanobacteria bacterium CYA TaxID=2484221 RepID=A0A5M4D386_9CYAN)

HSP 1 Score: 112 bits (280), Expect = 3.020e-28
Identity = 56/92 (60.87%), Postives = 66/92 (71.74%), Query Frame = 1
Query:    1 RTVFSCSVNAH--------PTEQG-RVEIRISGSGFLYNMVRIVAGALMEVGRGKIDPDTIPDIIASCDRTQAGPTLPPQGLRLEWIRYEEQ 249
            RT+  C V           P   G R+ IR+SGSGFLYNMVRIVAG L+EVGRG+ID D IP IIA+CDR  AGPTLPP+GLRLEW+RY ++
Sbjct:  205 RTISGCRVEQSARVDHLDGPAAPGERIVIRVSGSGFLYNMVRIVAGTLLEVGRGRIDADAIPRIIAACDRRAAGPTLPPEGLRLEWVRYGQE 296          
BLAST of mRNA_M-pyrifera_M_contig91295.21160.1 vs. uniprot
Match: A0A7V1MEG0_9BACT (tRNA pseudouridine synthase A n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7V1MEG0_9BACT)

HSP 1 Score: 110 bits (276), Expect = 5.930e-28
Identity = 54/82 (65.85%), Postives = 65/82 (79.27%), Query Frame = 1
Query:    1 RTVFSCSVNA--HPTEQGRVEIRISGSGFLYNMVRIVAGALMEVGRGKIDPDTIPDIIASCDRTQAGPTLPPQGLRLEWIRY 240
            RT++ CSV     P    R+ IR+SGSGFLYNMVRI+AG LM+VGRGKIDP ++ +I+AS DR +AG TLPPQGLRLEWIRY
Sbjct:  189 RTIYRCSVEQAESPNSGQRLVIRVSGSGFLYNMVRIIAGTLMDVGRGKIDPASMGEILASGDRRRAGVTLPPQGLRLEWIRY 270          
BLAST of mRNA_M-pyrifera_M_contig91295.21160.1 vs. uniprot
Match: A0A2E2N735_9BACT (tRNA pseudouridine synthase A n=1 Tax=Phycisphaerae bacterium TaxID=2026778 RepID=A0A2E2N735_9BACT)

HSP 1 Score: 111 bits (277), Expect = 6.690e-28
Identity = 53/86 (61.63%), Postives = 62/86 (72.09%), Query Frame = 1
Query:    1 RTVFSCSVNAHPTEQG--RVEIRISGSGFLYNMVRIVAGALMEVGRGKIDPDTIPDIIASCDRTQAGPTLPPQGLRLEWIRYEEQG 252
            RTVF+C V   P E    R    + GSGFLYNMVRI+ G L+EVGRGKI+PD +P IIAS DR  AGPT+ P+GLRLEWIRY  +G
Sbjct:  200 RTVFACDVATEPAEPNADRFVFGVEGSGFLYNMVRIIVGTLVEVGRGKIEPDDVPAIIASTDRRNAGPTMAPEGLRLEWIRYPGEG 285          
BLAST of mRNA_M-pyrifera_M_contig91295.21160.1 vs. uniprot
Match: A0A2A4RF22_9BACT (tRNA pseudouridine synthase A n=1 Tax=bacterium TaxID=1869227 RepID=A0A2A4RF22_9BACT)

HSP 1 Score: 106 bits (264), Expect = 4.220e-26
Identity = 48/82 (58.54%), Postives = 63/82 (76.83%), Query Frame = 1
Query:    1 RTVFSCSVNAHPTEQGRVEIRISGSGFLYNMVRIVAGALMEVGRGKIDPDTIPDIIASCDRTQAGPTLPPQGLRLEWIRYEE 246
            RT++ C +     + G+  +RISG+GFLYNMVRI+ G L+EVGRGKI+P+ + ++IAS DR  AG TLPP GLRLEW+RYEE
Sbjct:  196 RTIYKCEIET--PQPGKFVVRISGNGFLYNMVRIIGGTLVEVGRGKIEPEMVREMIASGDRKLAGVTLPPMGLRLEWVRYEE 275          
BLAST of mRNA_M-pyrifera_M_contig91295.21160.1 vs. uniprot
Match: A0A3L7TJ33_9BACT (tRNA pseudouridine synthase A n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3L7TJ33_9BACT)

HSP 1 Score: 105 bits (261), Expect = 1.240e-25
Identity = 51/80 (63.75%), Postives = 62/80 (77.50%), Query Frame = 1
Query:    1 RTVFSCSVNAHPTEQGRVEIRISGSGFLYNMVRIVAGALMEVGRGKIDPDTIPDIIASCDRTQAGPTLPPQGLRLEWIRY 240
            RTV  C+V   P  + R+EI I  +GFLYNMVRI+AG L+EVGRG+I+P+ IP IIA+CDR +AGPTLPPQGL L WI Y
Sbjct:  177 RTVLDCTVRT-PAPR-RIEIEICATGFLYNMVRIIAGTLLEVGRGRIEPEAIPAIIAACDRKRAGPTLPPQGLCLRWIWY 254          
BLAST of mRNA_M-pyrifera_M_contig91295.21160.1 vs. uniprot
Match: A0A7Y3I7G6_9BACT (tRNA pseudouridine synthase A n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7Y3I7G6_9BACT)

HSP 1 Score: 103 bits (257), Expect = 3.500e-25
Identity = 50/80 (62.50%), Postives = 61/80 (76.25%), Query Frame = 1
Query:    1 RTVFSCSVNAHPTEQGRVEIRISGSGFLYNMVRIVAGALMEVGRGKIDPDTIPDIIASCDRTQAGPTLPPQGLRLEWIRY 240
            RTV +C+V A  T   R+ I +SG+GFLYNMVRI+AG LM+VGRG   PD IP ++A+ DR  AGPTLPP+GLRLEWI Y
Sbjct:  177 RTVDACTVLA--TGPDRLRIDVSGNGFLYNMVRIIAGTLMQVGRGHWTPDVIPAVLAAADRVAAGPTLPPEGLRLEWIEY 254          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig91295.21160.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2D9CLI0_9BACT1.240e-3173.49tRNA pseudouridine synthase A n=1 Tax=Phycisphaera... [more]
A0A7C5WJG6_9BACT7.970e-3165.56tRNA pseudouridine synthase A n=1 Tax=Phycisphaera... [more]
A0A3M2BES3_9BACT1.560e-2963.10tRNA pseudouridine synthase A n=1 Tax=Planctomycet... [more]
A0A3M8FY44_9BACT1.160e-2867.47tRNA pseudouridine synthase A n=1 Tax=Phycisphaera... [more]
A0A5M4D386_9CYAN3.020e-2860.87tRNA pseudouridine synthase A n=1 Tax=Cyanobacteri... [more]
A0A7V1MEG0_9BACT5.930e-2865.85tRNA pseudouridine synthase A n=1 Tax=Phycisphaera... [more]
A0A2E2N735_9BACT6.690e-2861.63tRNA pseudouridine synthase A n=1 Tax=Phycisphaera... [more]
A0A2A4RF22_9BACT4.220e-2658.54tRNA pseudouridine synthase A n=1 Tax=bacterium Ta... [more]
A0A3L7TJ33_9BACT1.240e-2563.75tRNA pseudouridine synthase A n=1 Tax=Planctomycet... [more]
A0A7Y3I7G6_9BACT3.500e-2562.50tRNA pseudouridine synthase A n=1 Tax=Phycisphaera... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig91295contigM-pyrifera_M_contig91295:515..781 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score94.4
Seed ortholog evalue2.9e-17
Seed eggNOG ortholog1042156.CXIVA_06410
Preferred nametruA
KEGG koko:K06173
Hectar predicted targeting categoryother localisation
GOsGO:0001522,GO:0003674,GO:0003824,GO:0006139,GO:0006396,GO:0006399,GO:0006400,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0009451,GO:0009982,GO:0009987,GO:0010467,GO:0016070,GO:0016853,GO:0016866,GO:0031119,GO:0034470,GO:0034641,GO:0034660,GO:0043170,GO:0043412,GO:0044237,GO:0044238,GO:0046483,GO:0071704,GO:0090304,GO:1901360
EggNOG free text desc.Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs
EggNOG OGs1TQUY@1239,248W2@186801,36DP0@31979,COG0101@1,COG0101@2
EC5.4.99.12
COG Functional cat.J
Best tax levelClostridiaceae
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03016
Exons1
Model size267
Cds size267
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816825.5682774-CDS-M-pyrifera_M_contig91295:514..7811622816825.5682774-CDS-M-pyrifera_M_contig91295:514..781Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91295 515..781 -
1692278200.749259-CDS-M-pyrifera_M_contig91295:514..7811692278200.749259-CDS-M-pyrifera_M_contig91295:514..781Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91295 515..781 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig91295.21160.1prot_M-pyrifera_M_contig91295.21160.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig91295 515..781 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig91295.21160.1

>prot_M-pyrifera_M_contig91295.21160.1 ID=prot_M-pyrifera_M_contig91295.21160.1|Name=mRNA_M-pyrifera_M_contig91295.21160.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=89bp
RTVFSCSVNAHPTEQGRVEIRISGSGFLYNMVRIVAGALMEVGRGKIDPD
TIPDIIASCDRTQAGPTLPPQGLRLEWIRYEEQGHTTA*
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mRNA from alignment at M-pyrifera_M_contig91295:515..781-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig91295.21160.1 ID=mRNA_M-pyrifera_M_contig91295.21160.1|Name=mRNA_M-pyrifera_M_contig91295.21160.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=267bp|location=Sequence derived from alignment at M-pyrifera_M_contig91295:515..781- (Macrocystis pyrifera P11B4 male)
CGAACAGTCTTCAGCTGCTCTGTCAACGCGCATCCCACAGAACAGGGGCG CGTCGAGATCCGTATCAGCGGCTCAGGATTCCTCTACAACATGGTCCGCA TCGTCGCAGGCGCGCTTATGGAAGTCGGCCGCGGCAAGATCGATCCAGAC ACGATCCCAGACATCATCGCGTCTTGCGATCGCACGCAAGCTGGACCGAC CCTCCCGCCACAGGGTCTCCGCTTAGAATGGATCCGCTATGAAGAGCAAG GACACACGACGGCGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig91295:515..781-

>mRNA_M-pyrifera_M_contig91295.21160.1 ID=mRNA_M-pyrifera_M_contig91295.21160.1|Name=mRNA_M-pyrifera_M_contig91295.21160.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=534bp|location=Sequence derived from alignment at M-pyrifera_M_contig91295:515..781- (Macrocystis pyrifera P11B4 male)
CGAACAGTCTTCAGCTGCTCTGTCAACGCGCATCCCACAGAACAGGGGCG
CGTCGAGATCCGTATCAGCGGCTCAGGATTCCTCTACAACATGGTCCGCA
TCGTCGCAGGCGCGCTTATGGAAGTCGGCCGCGGCAAGATCGATCCAGAC
ACGATCCCAGACATCATCGCGTCTTGCGATCGCACGCAAGCTGGACCGAC
CCTCCCGCCACAGGGTCTCCGCTTAGAATGGATCCGCTATGAAGAGCAAG
GACACACGACGGCGTAACGAACAGTCTTCAGCTGCTCTGTCAACGCGCAT
CCCACAGAACAGGGGCGCGTCGAGATCCGTATCAGCGGCTCAGGATTCCT
CTACAACATGGTCCGCATCGTCGCAGGCGCGCTTATGGAAGTCGGCCGCG
GCAAGATCGATCCAGACACGATCCCAGACATCATCGCGTCTTGCGATCGC
ACGCAAGCTGGACCGACCCTCCCGCCACAGGGTCTCCGCTTAGAATGGAT
CCGCTATGAAGAGCAAGGACACACGACGGCGTAA
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