mRNA_M-pyrifera_M_contig91217.21139.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig91217.21139.1
Unique NamemRNA_M-pyrifera_M_contig91217.21139.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig91217.21139.1 vs. uniprot
Match: A0A838QZ41_9BACT (PQQ-dependent sugar dehydrogenase n=1 Tax=Pirellulales bacterium TaxID=2715253 RepID=A0A838QZ41_9BACT)

HSP 1 Score: 142 bits (359), Expect = 2.670e-36
Identity = 78/164 (47.56%), Postives = 111/164 (67.68%), Query Frame = 1
Query:   19 VYRVNAGGGEVAGSPVWSVDTLASPSPLLS---GSDRVASTGASIDVSGVD----APAEVFASERWDRSSGADMAWTFPVVDGEYEVRLFFAETYAPNFAAGSRVFDVELEGAVVLDGFDPFVAAGAGDTAVMESFVVSVVDGAIDVEFLRDVENPMVNAIEIL 489
            ++R+NAGG E+AGSPVW  DT A+PS   +   G+   A+T A+I+++        P  +F +ER+D+S   +M W FPV  G++EVRL+FAET++  FA G RVFDV++EGA+VLD +D F   GA +  VM SFVV+  D  +D++FLR V+NP +  IEIL
Sbjct:   34 LFRINAGGPELAGSPVWQADTAAAPSSFSNASAGNTATATTAATINMTHASLPLGTPMALFQAERFDKSGAPNMLWDFPVTPGQFEVRLYFAETWSGAFANGVRVFDVQIEGAMVLDNYDVFAEVGA-NKGVMNSFVVTS-DANLDIDFLRVVQNPAIKGIEIL 195          
BLAST of mRNA_M-pyrifera_M_contig91217.21139.1 vs. uniprot
Match: A0A517TWF5_9BACT (Di-glucose binding within endoplasmic reticulum n=1 Tax=Lacipirellula limnantheis TaxID=2528024 RepID=A0A517TWF5_9BACT)

HSP 1 Score: 136 bits (343), Expect = 3.780e-34
Identity = 78/165 (47.27%), Postives = 109/165 (66.06%), Query Frame = 1
Query:   19 VYRVNAGGGEVAGSPVWSVDTLASPSPLLS----GSDRVASTGASIDVSGVDAPA----EVFASERWDRSSGADMAWTFPVVDGEYEVRLFFAETYAPNFAAGSRVFDVELEGAVVLDGFDPFVAAGAGDTAVMESFVVSVVDGAIDVEFLRDVENPMVNAIEIL 489
            ++R+NAGG E+AGSP W  DTLA+PS   +    G+  V +  A+I++S    PA     +F +ER+D+S   ++ W FPV  G+YEVRL+FAET++  F  G RVFDV++EGA VLD +D F   GA +  V++SFVV+  DG +DV+F R  +NP V  IEIL
Sbjct:   38 LFRINAGGPELAGSPAWQADTLAAPSSYSNAAAGGNTGVTAVTAAINLSDPSLPAGTPQALFQTERFDKSGAPNLLWDFPVAPGQYEVRLYFAETWSGAFNNGVRVFDVKIEGATVLDDYDIFADVGA-NKGVVKSFVVTS-DGNLDVDFFRVTQNPAVKGIEIL 200          
BLAST of mRNA_M-pyrifera_M_contig91217.21139.1 vs. uniprot
Match: A0A7W0RZB1_9ACTN (Ig-like domain-containing protein (Fragment) n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A7W0RZB1_9ACTN)

HSP 1 Score: 132 bits (333), Expect = 8.120e-33
Identity = 77/167 (46.11%), Postives = 107/167 (64.07%), Query Frame = 1
Query:   10 GEVVYRVNAGGGEVAGSPVWSVDTLASPSPLLS---GSDRVASTGASIDVSG----VDAPAEVFASERWDRSSGADMAWTFPVVDGEYEVRLFFAETYAPNFAAGSRVFDVELEGAVVLDGFDPFVAAGAGDTAVMESFVVSVVDGAIDVEFLRDVENPMVNAIEIL 489
            G V++RVNAGG  VAG+P WS D+ A+PSP ++     +  A+TGA+ID S        PA +FA ER+D  +  +M W FPV  G +EVRL+FAET+    A G+R FDV +EG +VL+ +D F   GA +  V+++F VS  D  +D++F   VENP + AIEIL
Sbjct:  587 GTVLFRVNAGGPSVAGTPAWSEDSGAAPSPYVNTAAAGNSTAATGAAIDTSHPSVPAGTPAALFADERYDVDTAPEMEWNFPVTVGSHEVRLYFAETFGGTQAVGARKFDVTVEGQLVLNDYDVFAEVGA-NKGVVKTFTVSS-DANLDIDFGHVVENPSIKAIEIL 751          
BLAST of mRNA_M-pyrifera_M_contig91217.21139.1 vs. uniprot
Match: A0A317KFP3_9ACTN (Uncharacterized protein n=4 Tax=Micromonospora globispora TaxID=1450148 RepID=A0A317KFP3_9ACTN)

HSP 1 Score: 129 bits (325), Expect = 9.590e-32
Identity = 72/168 (42.86%), Postives = 105/168 (62.50%), Query Frame = 1
Query:   10 GEVVYRVNAGGGEVA---GSPVWSVDTLASPSPLLSGSDRVAS--TGASIDVS-GVDAPAEVFASERWDRSSGADMAWTFPVVDG-EYEVRLFFAETYAPNFAAGSRVFDVELEGAVVLDGFDPFVAAGAGDTAVMESFVVSVVDGAIDVEFLRDVENPMVNAIEILE 492
            G+V+YR+NAGG +VA   G P W+ DT  +PS   +    + +  T A++D S     P +VF+SERWD ++  +M+W FPV  G + +VRL+ A  Y      G R FDV L+GA+ L+ FDP  AAG  D  +M SF V + DG +D++F+  +ENP+V AIEI++
Sbjct:  465 GQVLYRINAGGPQVAAIDGGPAWAADTATAPSKYHNSGSNIQTYTTQATLDSSVPASTPVDVFSSERWDPTTAPEMSWDFPVAAGAQIQVRLYLANRYPTTNTVGKRKFDVWLDGALKLNDFDPVAAAGGTDRGIMRSFPV-ISDGNVDIDFIHVLENPLVQAIEIVQ 631          
BLAST of mRNA_M-pyrifera_M_contig91217.21139.1 vs. uniprot
Match: A0A518DDF6_9BACT (Di-glucose binding within endoplasmic reticulum n=1 Tax=Pirellulimonas nuda TaxID=2528009 RepID=A0A518DDF6_9BACT)

HSP 1 Score: 129 bits (325), Expect = 1.000e-31
Identity = 73/164 (44.51%), Postives = 104/164 (63.41%), Query Frame = 1
Query:    7 SGEVVYRVNAGGGEVAGSPVWSVDTL--ASPSPLLSGSDRVASTGASIDVSGVDAPAEVFASERWDRSSGADMAWTFPVVDGEYEVRLFFAETYAPNFAAGSRVFDVELEGAVVLDGFDPFVAAG-AGDTAVMESFVVSVVDGAIDVEFLRDVENPMVNAIEIL 489
            +G+VVYRVNAGGG++AG PVW  D+    +   L  G   +   G S+       PA +F +ER+D ++  +M+W FPV  G+YEVRL+FAET+  +  AG RVFDV +EG ++L+ +DPFVAAG A +  V E F +   D  I+++F R V+NP V   EI+
Sbjct:   36 AGDVVYRVNAGGGQLAGDPVWEGDSAQYVNTGALFGGGTNITLAG-SVPAG---TPAALFETERYDGTAAPEMSWAFPVTPGQYEVRLYFAETFG-SIGAGGRVFDVSIEGQLLLNDYDPFVAAGNALNRGVAEIFTIDA-DNVINIDFQRVVQNPAVKGFEII 193          
BLAST of mRNA_M-pyrifera_M_contig91217.21139.1 vs. uniprot
Match: A0A1G9TZL2_9EURY (REJ domain-containing protein n=1 Tax=Halogranum gelatinilyticum TaxID=660521 RepID=A0A1G9TZL2_9EURY)

HSP 1 Score: 128 bits (321), Expect = 3.420e-31
Identity = 82/174 (47.13%), Postives = 105/174 (60.34%), Query Frame = 1
Query:   10 GEVVYRVNAGGGEVA---GSPVWSVDTLASPSP-LLSGSDRVASTGA---SIDVS-GVDAPAEVFASERWDRSSGADMAWTFPVVDG-----EYEVRLFFAETYAPNFAAGSRVFDVELEGAVVLDGFDPFVAAGAGDTAVMESFVVSV-VDGAIDVEFLRDVENPMVNAIEIL 489
            G+V+YRVNAGG EVA   G P WS DT A+PS  L++G D   + G    S+D S   D P+E++ SERWD  +  +MAW F   DG      YEVRL+F + Y+     G RVFD+ +EG     GFDP  A G    A MESF V+   DG +D+ F+ DVENP +NAIEI+
Sbjct: 1206 GDVLYRVNAGGAEVAAADGGPAWSTDTEAAPSQYLVAGGDNTGAYGGGLNSVDSSVPADTPSELWNSERWDPPADPEMAWQF--ADGIEAGQTYEVRLYFYDGYSGTADPGDRVFDIVIEGTTQASGFDPIAAYG-DQNAGMESFTVTAGSDGDLDIVFIHDVENPQLNAIEIV 1376          
BLAST of mRNA_M-pyrifera_M_contig91217.21139.1 vs. uniprot
Match: A0A1I4C3Q0_9EURY (Di-glucose binding within endoplasmic reticulum n=3 Tax=Halogranum TaxID=695982 RepID=A0A1I4C3Q0_9EURY)

HSP 1 Score: 126 bits (316), Expect = 1.590e-30
Identity = 83/173 (47.98%), Postives = 106/173 (61.27%), Query Frame = 1
Query:   13 EVVYRVNAGGGEVAGS---PVWSVDTLASPSP-LLSGSDRVASTGA---SIDVSGVDA-PAEVFASERWDRSSGADMAWTFP--VVDGE-YEVRLFFAETYAPNFAAGSRVFDVELEGAVVLDGFDPFVAAGAGDTAVMESFVVSV-VDGAIDVEFLRDVENPMVNAIEILEA 495
            +V+YRVNAGG  V  +   P WS DT  SPS  L++G  +  S G+   SID S   + P  ++ SERWD+ SG +MAW F   +  G+ YEVRL+F + Y+   A G RVFDV +EG   L GF P  A G    A MES  V+   DG ID+EF+ DVENP +NAIEI+EA
Sbjct: 1139 QVLYRVNAGGSAVTATDDGPDWSADTGGSPSQYLVAGGTQTGSYGSGLNSIDDSVPSSTPTTIWNSERWDQPSGDEMAWEFADQIEAGQTYEVRLYFYDGYSGTSAVGDRVFDVNIEGTTELSGFSPIAAYG-DQNAGMESITVTAGSDGDIDIEFIHDVENPQINAIEIVEA 1310          
BLAST of mRNA_M-pyrifera_M_contig91217.21139.1 vs. uniprot
Match: J2ZCV1_9EURY (Uncharacterized protein n=2 Tax=Halogranum TaxID=695982 RepID=J2ZCV1_9EURY)

HSP 1 Score: 125 bits (315), Expect = 2.190e-30
Identity = 82/173 (47.40%), Postives = 100/173 (57.80%), Query Frame = 1
Query:   10 GEVVYRVNAGGGEVAGS---PVWSVDTLASPSPLLSGSDRVASTGASIDVSGVDA------PAEVFASERWDRSSGADMAWTFP--VVDGE-YEVRLFFAETYAPNFAAGSRVFDVELEGAVVLDGFDPFVAAGAGDTAVMESFVVSV-VDGAIDVEFLRDVENPMVNAIEIL 489
            G+V++RVN GG EV  +   PVWS DT  SPS  L     +   G +  V  VDA      P EVF  ER+D S   +MAW F   +  G+ YEVRL+F + YA   A G RVFDV +EG   L  FDP  A G   TA MES  V+   DG ID+EF+ DVENP +NAIEI+
Sbjct: 1207 GDVIHRVNVGGPEVVATDDGPVWSADTDGSPSQYLVAGGAI--PGGTYTVDAVDASVPSSTPNEVFQKERYDPSESPEMAWEFADQIEAGQTYEVRLYFYDGYAGTSAVGDRVFDVNIEGTTELQSFDPIAAYG-DQTAGMESVTVTAGSDGDIDIEFIHDVENPQINAIEIV 1376          
BLAST of mRNA_M-pyrifera_M_contig91217.21139.1 vs. uniprot
Match: A0A7W1SCG3_9BACT (Choice-of-anchor D domain-containing protein n=1 Tax=Acidobacteria bacterium TaxID=1978231 RepID=A0A7W1SCG3_9BACT)

HSP 1 Score: 125 bits (315), Expect = 2.240e-30
Identity = 75/167 (44.91%), Postives = 104/167 (62.28%), Query Frame = 1
Query:   16 VVYRVNAGGGEVAGSPVWSVDTLASPSPL---LSGSDRVASTGASIDVSGVDAPA----EVFASERWDRSSGADMAWTFPVVDGEYEVRLFFAETYAPNFAAGSRVFDVELEGAVVLDGFDPFVAAGAGDTAVMESFVVSVVDGAIDVEFLRDVENPMVNAIEILEA 495
            V+YRVNAGG EV+G+P WS DT ++PSP    ++ +    +T  ++D+     PA     +F +ER+ RS G DM W+FPV  G YEVRL+FAE Y  +   G+RVF V +EG  VL  +D     GA    V++SF V V D ++D+   R VENP +NAIEI++A
Sbjct:   39 VLYRVNAGGAEVSGTPSWSSDTASAPSPYSNAIASNSTTYTTKKAVDLGDPSIPAGTPGSLFQTERYPRSGGDDMRWSFPVTPGTYEVRLYFAEIYFTS--VGARVFSVAIEGDPVLSNYDIVADVGAFK-GVVKSFTV-VADASLDITLSRLVENPKINAIEIVQA 201          
BLAST of mRNA_M-pyrifera_M_contig91217.21139.1 vs. uniprot
Match: A0A5B1CIC7_9BACT (Di-glucose binding within endoplasmic reticulum n=3 Tax=Rubripirellula obstinata TaxID=406547 RepID=A0A5B1CIC7_9BACT)

HSP 1 Score: 125 bits (313), Expect = 4.200e-30
Identity = 70/164 (42.68%), Postives = 102/164 (62.20%), Query Frame = 1
Query:   19 VYRVNAGGGEVAGSPVWSVDTLASPSPLLSGS---DRVASTGASIDVSGVDAPA----EVFASERWDRSSGADMAWTFPVVDGEYEVRLFFAETYAPNFAAGSRVFDVELEGAVVLDGFDPFVAAGAGDTAVMESFVVSVVDGAIDVEFLRDVENPMVNAIEIL 489
            +YRVNAGG  +A +P W+ DT  + SP L+ +   + +AST   ID+S    P+     +F +ERWD+ +  DM W FPV  G YEVRLFFAET+    + G+R+FDV +EG  VLD +D +   G G T VM+SF+VS  D  ++++F    ++P V  IE++
Sbjct:  885 LYRVNAGGPNLASTPDWTTDTKVTWSPYLNAAAIGNTLASTTQPIDLSDPSIPSGTPQALFQTERWDQGADPDMKWEFPVTSGPYEVRLFFAETFTGTQSIGARIFDVSIEGQTVLDDYDVYADVG-GYTGVMKSFIVSS-DSVLNIDFGHVKQSPAVKGIEVI 1046          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig91217.21139.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A838QZ41_9BACT2.670e-3647.56PQQ-dependent sugar dehydrogenase n=1 Tax=Pirellul... [more]
A0A517TWF5_9BACT3.780e-3447.27Di-glucose binding within endoplasmic reticulum n=... [more]
A0A7W0RZB1_9ACTN8.120e-3346.11Ig-like domain-containing protein (Fragment) n=1 T... [more]
A0A317KFP3_9ACTN9.590e-3242.86Uncharacterized protein n=4 Tax=Micromonospora glo... [more]
A0A518DDF6_9BACT1.000e-3144.51Di-glucose binding within endoplasmic reticulum n=... [more]
A0A1G9TZL2_9EURY3.420e-3147.13REJ domain-containing protein n=1 Tax=Halogranum g... [more]
A0A1I4C3Q0_9EURY1.590e-3047.98Di-glucose binding within endoplasmic reticulum n=... [more]
J2ZCV1_9EURY2.190e-3047.40Uncharacterized protein n=2 Tax=Halogranum TaxID=6... [more]
A0A7W1SCG3_9BACT2.240e-3044.91Choice-of-anchor D domain-containing protein n=1 T... [more]
A0A5B1CIC7_9BACT4.200e-3042.68Di-glucose binding within endoplasmic reticulum n=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig91217contigM-pyrifera_M_contig91217:245..739 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score129.4
Seed ortholog evalue1.5e-27
Seed eggNOG ortholog1121405.dsmv_1062
KEGG rclassRC00508
KEGG koko:K00754,ko:K08678,ko:K13613
KEGG ReactionR01384
KEGG Pathwayko00520,ko01100,map00520,map01100
KEGG ModuleM00361
Hectar predicted targeting categoryother localisation
EggNOG free text desc.PFAM Di-glucose binding within endoplasmic reticulum
EggNOG OGs1R2HQ@1224,2WUV4@28221,42ZN7@68525,COG0451@1,COG0451@2,COG2133@1,COG2133@2
EC4.1.1.35
COG Functional cat.GM
CAZyGT4
Best tax levelDeltaproteobacteria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01004,ko01008
Exons1
Model size495
Cds size495
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816824.4780636-CDS-M-pyrifera_M_contig91217:244..7391622816824.4780636-CDS-M-pyrifera_M_contig91217:244..739Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91217 245..739 -
1692278200.1028047-CDS-M-pyrifera_M_contig91217:244..7391692278200.1028047-CDS-M-pyrifera_M_contig91217:244..739Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig91217 245..739 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig91217.21139.1prot_M-pyrifera_M_contig91217.21139.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig91217 245..739 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig91217.21139.1

>prot_M-pyrifera_M_contig91217.21139.1 ID=prot_M-pyrifera_M_contig91217.21139.1|Name=mRNA_M-pyrifera_M_contig91217.21139.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=165bp
GGSGEVVYRVNAGGGEVAGSPVWSVDTLASPSPLLSGSDRVASTGASIDV
SGVDAPAEVFASERWDRSSGADMAWTFPVVDGEYEVRLFFAETYAPNFAA
GSRVFDVELEGAVVLDGFDPFVAAGAGDTAVMESFVVSVVDGAIDVEFLR
DVENPMVNAIEILEA
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mRNA from alignment at M-pyrifera_M_contig91217:245..739-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig91217.21139.1 ID=mRNA_M-pyrifera_M_contig91217.21139.1|Name=mRNA_M-pyrifera_M_contig91217.21139.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=495bp|location=Sequence derived from alignment at M-pyrifera_M_contig91217:245..739- (Macrocystis pyrifera P11B4 male)
GGTGGATCGGGCGAGGTCGTGTATCGGGTGAATGCGGGTGGGGGTGAGGT TGCGGGTTCGCCGGTGTGGTCGGTGGATACGTTGGCGTCGCCGTCGCCGT TGTTGTCGGGGTCTGATCGTGTGGCGTCGACGGGTGCGTCGATTGATGTG TCGGGTGTGGATGCGCCGGCGGAGGTGTTTGCGTCGGAGCGTTGGGATCG TTCGTCGGGTGCGGATATGGCGTGGACGTTCCCGGTTGTTGATGGTGAGT ATGAGGTGCGGTTGTTCTTTGCGGAGACGTATGCGCCGAACTTTGCTGCG GGTTCTCGGGTGTTTGATGTGGAGCTCGAGGGTGCGGTGGTGTTGGACGG GTTTGATCCGTTTGTTGCTGCGGGTGCGGGTGATACTGCGGTGATGGAGT CGTTTGTGGTGTCGGTGGTTGATGGCGCGATCGATGTGGAGTTCTTGCGT GATGTGGAGAATCCGATGGTGAACGCCATCGAGATCCTCGAAGCC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig91217:245..739-

>mRNA_M-pyrifera_M_contig91217.21139.1 ID=mRNA_M-pyrifera_M_contig91217.21139.1|Name=mRNA_M-pyrifera_M_contig91217.21139.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=990bp|location=Sequence derived from alignment at M-pyrifera_M_contig91217:245..739- (Macrocystis pyrifera P11B4 male)
GGTGGATCGGGCGAGGTCGTGTATCGGGTGAATGCGGGTGGGGGTGAGGT
TGCGGGTTCGCCGGTGTGGTCGGTGGATACGTTGGCGTCGCCGTCGCCGT
TGTTGTCGGGGTCTGATCGTGTGGCGTCGACGGGTGCGTCGATTGATGTG
TCGGGTGTGGATGCGCCGGCGGAGGTGTTTGCGTCGGAGCGTTGGGATCG
TTCGTCGGGTGCGGATATGGCGTGGACGTTCCCGGTTGTTGATGGTGAGT
ATGAGGTGCGGTTGTTCTTTGCGGAGACGTATGCGCCGAACTTTGCTGCG
GGTTCTCGGGTGTTTGATGTGGAGCTCGAGGGTGCGGTGGTGTTGGACGG
GTTTGATCCGTTTGTTGCTGCGGGTGCGGGTGATACTGCGGTGATGGAGT
CGTTTGTGGTGTCGGTGGTTGATGGCGCGATCGATGTGGAGTTCTTGCGT
GATGTGGAGAATCCGATGGTGAACGCCATCGAGATCCTCGAAGCCGGTGG
ATCGGGCGAGGTCGTGTATCGGGTGAATGCGGGTGGGGGTGAGGTTGCGG
GTTCGCCGGTGTGGTCGGTGGATACGTTGGCGTCGCCGTCGCCGTTGTTG
TCGGGGTCTGATCGTGTGGCGTCGACGGGTGCGTCGATTGATGTGTCGGG
TGTGGATGCGCCGGCGGAGGTGTTTGCGTCGGAGCGTTGGGATCGTTCGT
CGGGTGCGGATATGGCGTGGACGTTCCCGGTTGTTGATGGTGAGTATGAG
GTGCGGTTGTTCTTTGCGGAGACGTATGCGCCGAACTTTGCTGCGGGTTC
TCGGGTGTTTGATGTGGAGCTCGAGGGTGCGGTGGTGTTGGACGGGTTTG
ATCCGTTTGTTGCTGCGGGTGCGGGTGATACTGCGGTGATGGAGTCGTTT
GTGGTGTCGGTGGTTGATGGCGCGATCGATGTGGAGTTCTTGCGTGATGT
GGAGAATCCGATGGTGAACGCCATCGAGATCCTCGAAGCC
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