mRNA_M-pyrifera_M_contig90433.20970.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig90433.20970.1
Unique NamemRNA_M-pyrifera_M_contig90433.20970.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig90433.20970.1 vs. uniprot
Match: A0A5A8D7X7_CAFRO (HAP2-GCS1 domain-containing protein n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8D7X7_CAFRO)

HSP 1 Score: 137 bits (345), Expect = 1.460e-35
Identity = 69/153 (45.10%), Postives = 96/153 (62.75%), Query Frame = 1
Query:   25 EGREYVMQDTLRISLSKSSIKLRYPLTHVREVNDHPFEVITRRDSEGVPFDLLINPCKEGLSSDAACGVFSYPDRDP-VPYSQGFCCRCSLEEFLSLPSTDGVLSRSNTDCRFSFAD-TQAAHCLRFSPLWHSLYSLGAPVVEYEITARVERC 477
            +G    ++D+L ++L+KSSI LRYPL + R  N  P E+I  RD +G  ++ L NPCK+G ++DAACG +  P     VPYSQGFCCRC   ++LS       LSR+N  C     +  Q+AHCLR+ PLW+  +SLG PVV + I A ++ C
Sbjct:   59 DGTRVELEDSLSVTLAKSSIVLRYPLQYERTYNADPRELILVRDGQGRDYNWLTNPCKDGTAADAACGSYVDPSTGKAVPYSQGFCCRCDFGDYLSGGPVG--LSRANLQCSLLSTELAQSAHCLRWGPLWYRAFSLGPPVVHFVIEAEIKFC 209          
BLAST of mRNA_M-pyrifera_M_contig90433.20970.1 vs. uniprot
Match: A0A5A8DA49_CAFRO (HAP2-GCS1 domain-containing protein n=4 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DA49_CAFRO)

HSP 1 Score: 138 bits (348), Expect = 6.290e-35
Identity = 69/153 (45.10%), Postives = 97/153 (63.40%), Query Frame = 1
Query:   25 EGREYVMQDTLRISLSKSSIKLRYPLTHVREVNDHPFEVITRRDSEGVPFDLLINPCKEGLSSDAACGVFSYPDRDP-VPYSQGFCCRCSLEEFLSLPSTDGVLSRSNTDCRFSFAD-TQAAHCLRFSPLWHSLYSLGAPVVEYEITARVERC 477
            +G    ++D+L ++L+KSSI LRYPL + R  N  P E+I  RD +G  ++ L NPCK+G ++DAACG ++ P     VPYSQGFCCRC   ++LS       LSR+N  C     +  Q+AHCLR+ PLW+  +SLG PVV + I A ++ C
Sbjct:   59 DGTRVELEDSLSVTLAKSSIVLRYPLQYERTYNADPRELILVRDGQGRDYNWLTNPCKDGTAADAACGSYADPSTGKAVPYSQGFCCRCDFGDYLSGGPVG--LSRANLQCSLLSTELAQSAHCLRWGPLWYRAFSLGPPVVHFVIEAEIKFC 209          
BLAST of mRNA_M-pyrifera_M_contig90433.20970.1 vs. uniprot
Match: A0A6T6MS87_9RHOD (Hypothetical protein n=1 Tax=Timspurckia oligopyrenoides TaxID=708627 RepID=A0A6T6MS87_9RHOD)

HSP 1 Score: 119 bits (298), Expect = 3.100e-28
Identity = 64/150 (42.67%), Postives = 95/150 (63.33%), Query Frame = 1
Query:   58 RISLSKSSIKLRYPLTHVREVNDHPFEVITRRDSEGVPFDLLINPCKEGLSSDAA-CGVFSYP--DRDP---VPYSQGFCCRCSLEEFLSLPSTDGVLSRSNTDCRF----SFADTQAAHCLRFSPLWHSLYSLGAPVVEYEITARVERC 477
            +I +SKS I+LRYPL +VR+VN+ P+E I+++ + G PF+ L NPC+ G + D+A CG F  P   RD    V  SQGFCC C L ++L + S    ++R+  +C F      +   ++HCLRF PLW+S++S+GAP V ++I   V  C
Sbjct:  109 KIQISKSKIRLRYPLYYVRDVNNLPYE-ISKQSNSGGPFNWLYNPCRAGWNDDSASCGFFYDPPSSRDAEHRVWDSQGFCCECGLNDYLGIGSD---VARAAKECSFFSVFGSSSDHSSHCLRFDPLWYSVFSIGAPDVLFDIQLNVSTC 254          
BLAST of mRNA_M-pyrifera_M_contig90433.20970.1 vs. uniprot
Match: A0A5J4YWR1_PORPP (Protein HAPLESS 2-A n=1 Tax=Porphyridium purpureum TaxID=35688 RepID=A0A5J4YWR1_PORPP)

HSP 1 Score: 115 bits (289), Expect = 5.240e-27
Identity = 64/157 (40.76%), Postives = 89/157 (56.69%), Query Frame = 1
Query:   37 YVMQDTLRISLSKSSIKLRYPLTHVREVNDHPFEVITRRDSEGVPFDLLINPCKEGLSSDAA-CGVFSYPDR-----DPVPYSQGFCCRCSLEEFLSLPSTDGVLSRSNTDCRF----SFADTQAAHCLRFSPLWHSLYSLGAPVVEYEITARVERC 477
            Y  +    IS+S+S I LRYPL ++R+VN+ PFEV  + ++ G PF+ L NPC++G   DAA CG F  P       D V  SQGFCC+C L+++L    T     RS  +C F      +   +AHCLRF  LW+S + + AP V Y +T  +  C
Sbjct:   99 YKFRQRSEISMSRSKIVLRYPLFYLRDVNNKPFEVTKQTNTNG-PFNWLYNPCRDGWEDDAASCGFFYDPPSSRNADDRVWDSQGFCCKCGLDDYLG---TSNDAPRSAIECSFFSVFGGSSDYSAHCLRFGNLWYSAFEVRAPDVLYTVTLNITHC 251          
BLAST of mRNA_M-pyrifera_M_contig90433.20970.1 vs. uniprot
Match: M2XN01_GALSU (HAP2-GCS1 domain-containing protein n=1 Tax=Galdieria sulphuraria TaxID=130081 RepID=M2XN01_GALSU)

HSP 1 Score: 99.4 bits (246), Expect = 1.860e-22
Identity = 51/144 (35.42%), Postives = 83/144 (57.64%), Query Frame = 1
Query:   19 DEEGREYVMQDTLRISLSKSSIKLRYPLTHVREVNDHPFEVITRRDSEGVPFDLLINPCKEGL-SSDAACGVFSYPDRD-----PVPYSQGFCCRCSLEEFLSLPSTDGVLSRSNTDCRFSFADTQAAHCLRFSPLWHSLYSLG 432
            ++  + Y+   TL I+LSKS I + YPL ++++ N+ P+E++   +S G P +   NPC +   SS+  CG    P  +      +  SQGFCC+C + ++L+  + +G LS +     F    +QAAHCLRF PLW+S + +G
Sbjct:   84 NDPNKAYLFNYTLHITLSKSKIAIEYPLVYLQDFNNQPYEIVYEANSNG-PLEEYTNPCVDSWGSSNPTCGYAYDPPNEIDAANRIYNSQGFCCQCGVSDYLAEGTREG-LSCNLLGSLFQIEPSQAAHCLRFDPLWYSGFQIG 225          
BLAST of mRNA_M-pyrifera_M_contig90433.20970.1 vs. uniprot
Match: M1V8A6_CYAM1 (HAP2-GCS1 domain-containing protein n=1 Tax=Cyanidioschyzon merolae (strain NIES-3377 / 10D) TaxID=280699 RepID=M1V8A6_CYAM1)

HSP 1 Score: 96.3 bits (238), Expect = 3.650e-20
Identity = 57/161 (35.40%), Postives = 84/161 (52.17%), Query Frame = 1
Query:   19 DEEGREYVMQDTLRISLSKSSIKLRYPLTHVREVNDHPFEVITRRDSEGVPFDLLINPCKEGL-SSDAACGVFSYPD-----RDPVPYSQGFCCRCSLEEFLSLPSTDGVLSRSNTDCRFSFAD--TQAAHCLRFSPLWHSLYSLGAPVVEYEITARVERC 477
            D     Y  +  + I+L+KS I+L YPL +  + N+ P+E++ + + +G P + L N C     SSD  CG    P       D + YSQGFCC C+  + L L S + +  R   DC     D  T++AHCLRF  LW+S + +G P V + I   V +C
Sbjct:   95 DNPNTVYAFKYQVHITLTKSRIRLDYPLYYQSDFNNKPYEIVYKYNQKG-PLNWLDNQCVATWGSSDPTCGYAYNPSWSTKPADRILYSQGFCCDCNAGDLLGL-SPNRI--RGGLDCSLLNFDNPTESAHCLRFDSLWYSAFQIGEPDVNFVILVNVTKC 251          
BLAST of mRNA_M-pyrifera_M_contig90433.20970.1 vs. uniprot
Match: E9B5T2_LEIMU (HAP2-GCS1 domain-containing protein n=1 Tax=Leishmania mexicana (strain MHOM/GT/2001/U1103) TaxID=929439 RepID=E9B5T2_LEIMU)

HSP 1 Score: 90.9 bits (224), Expect = 2.870e-18
Identity = 51/141 (36.17%), Postives = 75/141 (53.19%), Query Frame = 1
Query:   55 LRISLSKSSIKLRYPLTHVREVNDHPFEVITRRDSEGVPFDLLINPCKEGLSSD-AACGVFSYPDRDPVPYSQGFCCRCSLEEFLSLPSTDGVLSRSNTDCRFSFADTQAAHCLRFSPLWHSLYSLGAPVVEYEITARVER 474
            LRI+ S+S+++ RYPL +V+  N  P+E   R +        L+N C    ++D A CG+       P+PYSQGFCC CS+ + L L   D   +R+N  C   F    AA CLRF   W+S Y++G  +  Y +   + R
Sbjct:  192 LRITTSRSAVRYRYPLFYVQNYNAKPYEATVRGN--------LLNQCNADFNADKATCGLAYDAAGKPIPYSQGFCCDCSMCQTLGLCKPD---ARANAACNI-FDKYTAASCLRFGQRWYSGYTIGGYMTWYTVNLTLSR 320          
BLAST of mRNA_M-pyrifera_M_contig90433.20970.1 vs. uniprot
Match: A0A7S4L9V6_9EUGL (Hypothetical protein (Fragment) n=1 Tax=Eutreptiella gymnastica TaxID=73025 RepID=A0A7S4L9V6_9EUGL)

HSP 1 Score: 89.7 bits (221), Expect = 6.960e-18
Identity = 51/152 (33.55%), Postives = 75/152 (49.34%), Query Frame = 1
Query:   19 DEEGREYVMQDTLRISLSKSSIKLRYPLTHVREVNDHPFEVITRRDSEGVPFDLLINPCKEGLSS-DAACGVFSYPDRDPVPYSQGFCCRCSLEEFLSLPSTDGVLSRSNTDCRFSFADTQAAHCLRFSPLWHSLYSLGAPVVEYEITARVE 471
            D  G    + D +++ +SKS+   RYPLT+V++ N +P E ++          LL   C++  S  +  CG        P+PYSQGFCCRC   E L+    D   SR   DC   F    +AHCL +   W+S +++G     Y IT  +E
Sbjct:   76 DRTGVNRTLSDPVKVRISKSTSMFRYPLTYVQDFNANPHETVSHTS-------LL--QCRDDSSDVEPTCGWHMDSQGQPIPYSQGFCCRCPASELLNF--FDPADSRGQVDCSL-FGKAASAHCLDWGDDWYSAFAVGPARTWYSITVSLE 215          
BLAST of mRNA_M-pyrifera_M_contig90433.20970.1 vs. uniprot
Match: A0A0N0P372_LEPSE (HAP2-GCS1 domain-containing protein n=1 Tax=Leptomonas seymouri TaxID=5684 RepID=A0A0N0P372_LEPSE)

HSP 1 Score: 88.6 bits (218), Expect = 1.850e-17
Identity = 49/141 (34.75%), Postives = 76/141 (53.90%), Query Frame = 1
Query:   55 LRISLSKSSIKLRYPLTHVREVNDHPFEVITRRDSEGVPFDLLINPCKEGLSSD-AACGVFSYPDRDPVPYSQGFCCRCSLEEFLSLPSTDGVLSRSNTDCRFSFADTQAAHCLRFSPLWHSLYSLGAPVVEYEITARVER 474
            + I+ S+S+++ RYP+ +V+  N  P+E   +          ++N C   L +D A CGV   P R  +P+SQGFCC CS+ + L     +   +R+N  C    A T A+ CLRF P W+S Y++GA V  + +   V R
Sbjct:  213 ISITTSRSAVRYRYPIFYVQNYNAKPYEATVK--------GKMLNQCNAELDADKATCGVALDPTRKAIPFSQGFCCDCSMCQTLGFCEPN---ARANMACNVFDAYTTAS-CLRFGPRWYSGYTIGAYVTWFTLNVTVSR 341          
BLAST of mRNA_M-pyrifera_M_contig90433.20970.1 vs. uniprot
Match: A0A0K0VK76_ANDGO (HAP2-like protein n=1 Tax=Andalucia godoyi TaxID=505711 RepID=A0A0K0VK76_ANDGO)

HSP 1 Score: 87.4 bits (215), Expect = 4.610e-17
Identity = 47/153 (30.72%), Postives = 82/153 (53.59%), Query Frame = 1
Query:   19 DEEGREYVMQDTLRISLSKSSIKLRYPLTHVREVNDHPFEVITRRDSEGVPFDLLINPCKE-GLSSDAACGVFSYPDRDPVPYSQGFCCRCSLEEFLSLPSTDGVLSRSNTDCRFSFADT-QAAHCLRFSPLWHSLYSLGAPVVEYEITARVE 471
            D +GR Y +   ++IS+S++S  L YP+T+ +  N  P+E + +  + G         C + G +S  +CG     + + +P SQGFCC C+L + ++   T     R++  C   F  + Q+AHCLR+  LW++ Y +G P + + +   VE
Sbjct:   78 DSQGRNYTLASPVKISVSRTSSYLSYPITYRQTFNAAPYEQVIKLGAFG---------CTDSGGASSPSCGWAQDSNGNNIPDSQGFCCSCTLMDAVNSQRT-----RASLSCLTGFLTSYQSAHCLRYDTLWYAAYEIGDPTLFFNVNVAVE 216          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig90433.20970.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 21
Match NameE-valueIdentityDescription
A0A5A8D7X7_CAFRO1.460e-3545.10HAP2-GCS1 domain-containing protein n=1 Tax=Cafete... [more]
A0A5A8DA49_CAFRO6.290e-3545.10HAP2-GCS1 domain-containing protein n=4 Tax=Cafete... [more]
A0A6T6MS87_9RHOD3.100e-2842.67Hypothetical protein n=1 Tax=Timspurckia oligopyre... [more]
A0A5J4YWR1_PORPP5.240e-2740.76Protein HAPLESS 2-A n=1 Tax=Porphyridium purpureum... [more]
M2XN01_GALSU1.860e-2235.42HAP2-GCS1 domain-containing protein n=1 Tax=Galdie... [more]
M1V8A6_CYAM13.650e-2035.40HAP2-GCS1 domain-containing protein n=1 Tax=Cyanid... [more]
E9B5T2_LEIMU2.870e-1836.17HAP2-GCS1 domain-containing protein n=1 Tax=Leishm... [more]
A0A7S4L9V6_9EUGL6.960e-1833.55Hypothetical protein (Fragment) n=1 Tax=Eutreptiel... [more]
A0A0N0P372_LEPSE1.850e-1734.75HAP2-GCS1 domain-containing protein n=1 Tax=Leptom... [more]
A0A0K0VK76_ANDGO4.610e-1730.72HAP2-like protein n=1 Tax=Andalucia godoyi TaxID=5... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig90433contigM-pyrifera_M_contig90433:201..677 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score95.5
Seed ortholog evalue2.4e-17
Seed eggNOG ortholog45157.CMK076CT
Preferred nameGCS1
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0003006,GO:0003674,GO:0005488,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005886,GO:0005887,GO:0007275,GO:0007276,GO:0007338,GO:0007342,GO:0008104,GO:0008150,GO:0008289,GO:0009555,GO:0009566,GO:0009567,GO:0009987,GO:0012505,GO:0016020,GO:0016021,GO:0016043,GO:0019953,GO:0022412,GO:0022414,GO:0022607,GO:0030154,GO:0031224,GO:0031226,GO:0031253,GO:0031410,GO:0031982,GO:0032501,GO:0032502,GO:0032504,GO:0033036,GO:0034613,GO:0042995,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043933,GO:0044085,GO:0044424,GO:0044425,GO:0044444,GO:0044459,GO:0044463,GO:0044464,GO:0044703,GO:0045026,GO:0045184,GO:0048229,GO:0048232,GO:0048235,GO:0048609,GO:0048856,GO:0048869,GO:0051179,GO:0051205,GO:0051234,GO:0051259,GO:0051260,GO:0051641,GO:0051704,GO:0055046,GO:0061024,GO:0061025,GO:0061936,GO:0065003,GO:0070206,GO:0070207,GO:0070727,GO:0071840,GO:0071944,GO:0072657,GO:0090150,GO:0097708,GO:0098590,GO:0120025,GO:0120038
EggNOG free text desc.single fertilization
EggNOG OGs28J2C@1,2QREH@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons1
Model size477
Cds size477
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816816.4662886-CDS-M-pyrifera_M_contig90433:200..6771622816816.4662886-CDS-M-pyrifera_M_contig90433:200..677Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig90433 201..677 -
1692278195.415868-CDS-M-pyrifera_M_contig90433:200..6771692278195.415868-CDS-M-pyrifera_M_contig90433:200..677Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig90433 201..677 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig90433.20970.1prot_M-pyrifera_M_contig90433.20970.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig90433 201..677 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig90433.20970.1

>prot_M-pyrifera_M_contig90433.20970.1 ID=prot_M-pyrifera_M_contig90433.20970.1|Name=mRNA_M-pyrifera_M_contig90433.20970.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=159bp
MGNACCDEEGREYVMQDTLRISLSKSSIKLRYPLTHVREVNDHPFEVITR
RDSEGVPFDLLINPCKEGLSSDAACGVFSYPDRDPVPYSQGFCCRCSLEE
FLSLPSTDGVLSRSNTDCRFSFADTQAAHCLRFSPLWHSLYSLGAPVVEY
EITARVERC
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mRNA from alignment at M-pyrifera_M_contig90433:201..677-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig90433.20970.1 ID=mRNA_M-pyrifera_M_contig90433.20970.1|Name=mRNA_M-pyrifera_M_contig90433.20970.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=477bp|location=Sequence derived from alignment at M-pyrifera_M_contig90433:201..677- (Macrocystis pyrifera P11B4 male)
ATGGGGAATGCTTGTTGTGATGAGGAAGGGAGAGAGTATGTGATGCAAGA CACACTTCGTATTTCTTTGTCAAAGTCTAGCATCAAGCTTCGGTACCCTT TAACGCATGTGAGAGAAGTGAATGACCATCCATTCGAAGTGATTACGAGG CGCGACTCGGAGGGTGTGCCGTTCGACTTGCTGATTAACCCATGCAAAGA AGGGTTGTCATCAGATGCAGCATGTGGCGTTTTTTCTTACCCTGATCGCG ATCCAGTGCCTTATTCGCAGGGCTTTTGCTGCCGTTGTTCCTTGGAAGAG TTTTTGAGCCTTCCTTCTACCGATGGAGTATTGTCACGGAGCAATACAGA CTGCAGATTCTCGTTTGCAGACACCCAGGCGGCGCACTGTCTCCGTTTCT CGCCATTGTGGCATTCTCTATACTCCTTGGGAGCCCCAGTGGTGGAATAT GAGATAACTGCACGGGTGGAGCGCTGC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig90433:201..677-

>mRNA_M-pyrifera_M_contig90433.20970.1 ID=mRNA_M-pyrifera_M_contig90433.20970.1|Name=mRNA_M-pyrifera_M_contig90433.20970.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=954bp|location=Sequence derived from alignment at M-pyrifera_M_contig90433:201..677- (Macrocystis pyrifera P11B4 male)
ATGGGGAATGCTTGTTGTGATGAGGAAGGGAGAGAGTATGTGATGCAAGA
CACACTTCGTATTTCTTTGTCAAAGTCTAGCATCAAGCTTCGGTACCCTT
TAACGCATGTGAGAGAAGTGAATGACCATCCATTCGAAGTGATTACGAGG
CGCGACTCGGAGGGTGTGCCGTTCGACTTGCTGATTAACCCATGCAAAGA
AGGGTTGTCATCAGATGCAGCATGTGGCGTTTTTTCTTACCCTGATCGCG
ATCCAGTGCCTTATTCGCAGGGCTTTTGCTGCCGTTGTTCCTTGGAAGAG
TTTTTGAGCCTTCCTTCTACCGATGGAGTATTGTCACGGAGCAATACAGA
CTGCAGATTCTCGTTTGCAGACACCCAGGCGGCGCACTGTCTCCGTTTCT
CGCCATTGTGGCATTCTCTATACTCCTTGGGAGCCCCAGTGGTGGAATAT
GAGATAACTGCACGGGTGGAGCGCTGCATGGGGAATGCTTGTTGTGATGA
GGAAGGGAGAGAGTATGTGATGCAAGACACACTTCGTATTTCTTTGTCAA
AGTCTAGCATCAAGCTTCGGTACCCTTTAACGCATGTGAGAGAAGTGAAT
GACCATCCATTCGAAGTGATTACGAGGCGCGACTCGGAGGGTGTGCCGTT
CGACTTGCTGATTAACCCATGCAAAGAAGGGTTGTCATCAGATGCAGCAT
GTGGCGTTTTTTCTTACCCTGATCGCGATCCAGTGCCTTATTCGCAGGGC
TTTTGCTGCCGTTGTTCCTTGGAAGAGTTTTTGAGCCTTCCTTCTACCGA
TGGAGTATTGTCACGGAGCAATACAGACTGCAGATTCTCGTTTGCAGACA
CCCAGGCGGCGCACTGTCTCCGTTTCTCGCCATTGTGGCATTCTCTATAC
TCCTTGGGAGCCCCAGTGGTGGAATATGAGATAACTGCACGGGTGGAGCG
CTGC
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