mRNA_M-pyrifera_M_contig90357.20948.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig90357.20948.1
Unique NamemRNA_M-pyrifera_M_contig90357.20948.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig90357.20948.1 vs. uniprot
Match: A0A5A8D0R5_CAFRO (Uncharacterized protein n=3 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8D0R5_CAFRO)

HSP 1 Score: 137 bits (345), Expect = 2.400e-35
Identity = 79/147 (53.74%), Postives = 96/147 (65.31%), Query Frame = 1
Query:    1 ERRVFDLPWRAKLRGCRRFRWRGNLFYREGQFARAAVQYEAALAWYEYTFPESS---EGTAAVDAERVSALCNLAACQLQLRKYKDAEGSCDQALHMDAHCIKALFRRAVARRHQFLFEGAAADLRTLHSLLTPPPPPLVREFRLLR 432
            ERRVFDLP   K+ G RRF  RGNL + EGQF RAAVQ+ AAL WYEYTFP+     +G AA+DA R + L NLAAC++ LR +K+AE +  QAL      +KAL+RRA+ARRH   FE AAAD+    +   P    L RE  LLR
Sbjct:   79 ERRVFDLPTSHKMSGIRRFHARGNLLFSEGQFRRAAVQFRAALVWYEYTFPDQRKGPDGQAALDARRAACLLNLAACEISLRAWKEAEETATQALRALPGNVKALYRRALARRHLHRFEAAAADVEAALAA-APGSGALRREAALLR 224          
BLAST of mRNA_M-pyrifera_M_contig90357.20948.1 vs. uniprot
Match: A0A662XSD5_9STRA (Uncharacterized protein n=1 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662XSD5_9STRA)

HSP 1 Score: 105 bits (261), Expect = 1.020e-23
Identity = 68/170 (40.00%), Postives = 95/170 (55.88%), Query Frame = 1
Query:    1 ERRVFDLPWRAKLRGCRRFRWRGNLFYREGQFARAAVQYEAALAWYEYTFPESSEGTAAVDAERVSALCNLAACQLQLRKYKDAEGSCDQALHMDAHCIKALFRRAVARRHQFLFEGAAADL-RTLHSLLTPPPPP-----LVREFRLLRAARAVYRKRKAQMSKSMFQG 492
            E+++ D+  + KL  C  FR  GNLF+R GQ+ RAA  Y  AL ++EY FP++ E     DA ++  L N AAC+L+     DA    +QAL +D   +KAL+RRA A R +  FE A+ D+ R L    T    P     LV+E  LL+A    Y+ R  Q+S +MF G
Sbjct:  142 EQKLMDMSTQEKLTACDEFRVLGNLFFRHGQYQRAAYNYHKALVYFEYVFPDTEEEDIQADALKLKLLLNFAACRLKTLHLDDAIHHANQALQLDPDNVKALYRRAQAYRLKDEFELASQDISRALELSGTSENAPTSDPLLVQEKALLQAKMLAYKLRTKQVSTAMFGG 311          
BLAST of mRNA_M-pyrifera_M_contig90357.20948.1 vs. uniprot
Match: A0A662XGD2_9STRA (Uncharacterized protein n=1 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662XGD2_9STRA)

HSP 1 Score: 104 bits (259), Expect = 1.790e-23
Identity = 65/168 (38.69%), Postives = 92/168 (54.76%), Query Frame = 1
Query:    7 RVFDLPWRAKLRGCRRFRWRGNLFYREGQFARAAVQYEAALAWYEYTFPESSEGTAAVDAERVSALCNLAACQLQLRKYKDAEGSCDQALHMDAHCIKALFRRAVARRHQFLFEGAAADLRTLHSL------LTPPPPPLVREFRLLRAARAVYRKRKAQMSKSMFQG 492
            ++ D+  + KL  C  FR  GNLF+R GQ+ RAA  Y  AL ++EY FP++ +  A  DA ++  L N AAC+L+     DA    +QAL +D   +KAL+RRA A R +  FE A+ D+     L           P LV+E  LL+A    Y+ R  Q+S +MF G
Sbjct:  136 KLMDMSTQEKLTACDEFRVLGNLFFRHGQYQRAAYNYHKALVYFEYVFPDTEDEEAQADALKLKLLLNFAACRLKTLHLDDAVHHANQALQLDPDNVKALYRRAQAHRLKDEFELASQDISRALELSGTSEDAQTADPLLVQEKALLQAKILAYKLRTKQVSTAMFGG 303          
BLAST of mRNA_M-pyrifera_M_contig90357.20948.1 vs. uniprot
Match: A0A7S2RXA3_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2RXA3_9STRA)

HSP 1 Score: 102 bits (254), Expect = 2.650e-22
Identity = 61/164 (37.20%), Postives = 87/164 (53.05%), Query Frame = 1
Query:    1 ERRVFDLPWRAKLRGCRRFRWRGNLFYREGQFARAAVQYEAALAWYEYTFPESSEGTAAVDAERVSALCNLAACQLQLRKYKDAEGSCDQALHMDAHCIKALFRRAVARRHQFLFEGAAADLRTLHSLLTPPPPPLVREFRLLRAARAVYRKRKAQMSKSMFQG 492
            ER +F+LP   K+     +   GNLFY EG + RA ++Y +ALA+YEYTFPE       +D  R+    N AAC L+LR  ++A   C+Q L+ +   +KAL+RRA   RH   +  A  D+    SL  P       EF  L+     YR+  A ++K+M  G
Sbjct:  169 EREIFELPLETKISRMEDYHSMGNLFYDEGLYDRAVIEYRSALAYYEYTFPEDDATWQLIDKLRLVCHLNSAACHLKLRMLQEALDDCNQVLYAEPENLKALYRRAQVYRHMDKYTEARRDITRALSL-APHDFQAREEFVRLKTKMHAYRQNSAILAKAMLHG 331          
BLAST of mRNA_M-pyrifera_M_contig90357.20948.1 vs. uniprot
Match: D0NSG6_PHYIT (Uncharacterized protein n=2 Tax=Phytophthora infestans TaxID=4787 RepID=D0NSG6_PHYIT)

HSP 1 Score: 97.1 bits (240), Expect = 4.960e-21
Identity = 61/168 (36.31%), Postives = 92/168 (54.76%), Query Frame = 1
Query:    1 ERRVFDLPWRAKLRGCRRFRWRGNLFYREGQFARAAVQYEAALAWYEYTFPESSEGTAAVDAERVSALCNLAACQLQLRKYKDAEGSCDQALHMDAHCIKALFRRAVARRHQFLFEGAAADLRTLHSL--LTPPPPP----LVREFRLLRAARAVYRKRKAQMSKSMF 486
            E+++ D+  + K   C   R  GNLF++ GQ+ RAA  Y  AL ++EY FP++ E  A  DA ++  L N AAC+L+     DA    +QAL ++ + +KAL+RRA A R +  F  A  D+     L   T   P     L++E +LL+A    Y+ R  Q+S +MF
Sbjct:   86 EQKLMDMTTQEKTTACDELRVLGNLFFKHGQYQRAAFHYHKALVYFEYVFPDTDEEEAQADALKLKLLLNFAACRLKTLHLDDAVHHANQALEINTNNVKALYRRAQANRLKDEFHLAQKDIERAIKLSNATEDTPSIDSLLLQEKKLLQAKMLAYKLRTKQVSAAMF 253          
BLAST of mRNA_M-pyrifera_M_contig90357.20948.1 vs. uniprot
Match: A0A812Y143_SYMMI (FKBP4 protein n=1 Tax=Symbiodinium microadriaticum TaxID=2951 RepID=A0A812Y143_SYMMI)

HSP 1 Score: 89.4 bits (220), Expect = 1.450e-19
Identity = 52/139 (37.41%), Postives = 74/139 (53.24%), Query Frame = 1
Query:   10 VFDLPWRAKLRGCRRFRWRGNLFYREGQFARAAVQYEAALAWYEYTFPESSEGTAAVDAERVSALCNLAACQLQLRKYKDAEGSCDQALHM---------DAHCIKALFRRAVARRHQFLFEGAAADLRTLHSLLTPPP 399
            +F LP   K+R C R R  GNL Y EG   +AA QY+ AL++YEY FPE  E   ++D  R +ALCN++ C  ++   ++AE +  QA+           +    KA +RRA   R    +E A ADLR + +L    P
Sbjct:    1 MFHLPETDKIRFCERHRILGNLLYEEGLLPKAAEQYQTALSYYEYCFPEDDETQRSLDTVRYAALCNISLCYCRMGLLREAEAAATQAVRESEDRRSTLPEVARAKAYYRRAQVHRKLDDYESAMADLRRVLALCPDDP 139          
BLAST of mRNA_M-pyrifera_M_contig90357.20948.1 vs. uniprot
Match: A0A6A3FIC8_9STRA (Uncharacterized protein n=4 Tax=Phytophthora TaxID=4783 RepID=A0A6A3FIC8_9STRA)

HSP 1 Score: 88.6 bits (218), Expect = 8.900e-18
Identity = 60/168 (35.71%), Postives = 84/168 (50.00%), Query Frame = 1
Query:    1 ERRVFDLPWRAKLRGCRRFRWRGNLFYREGQFARAAVQYEAALAWYEYTFPESSEGTAAVDAERVSALCNLAACQLQLRKYKDAEGSCDQALHMDAHCIKALFRRAVARRHQFLFEGAAADLRTLHSLLTPPPPP------LVREFRLLRAARAVYRKRKAQMSKSMF 486
            E+++ D+    KLR C  FR  GNLF++ GQ+ RAA  Y  AL ++EY F +               L N AAC+L+     DA    +QAL +DA  +KAL+RRA A R +  F+ A  D+     L             LV+E  LL+A    Y+ R  Q+S +MF
Sbjct:  104 EQKLMDMTAHEKLRACDEFRVLGNLFFKHGQYQRAAFHYHKALVYFEYVFTDXXXXXXXXXXXXXXLLLNFAACRLKTLHLDDAVHHANQALEIDADNVKALYRRAQAYRLKDEFDLAQQDITRAIELSKAAGDSQSTDAMLVQEKTLLQAKMLAYKLRTKQVSAAMF 271          
BLAST of mRNA_M-pyrifera_M_contig90357.20948.1 vs. uniprot
Match: G4ZJT9_PHYSP (Uncharacterized protein n=1 Tax=Phytophthora sojae (strain P6497) TaxID=1094619 RepID=G4ZJT9_PHYSP)

HSP 1 Score: 87.4 bits (215), Expect = 1.950e-17
Identity = 59/168 (35.12%), Postives = 84/168 (50.00%), Query Frame = 1
Query:    1 ERRVFDLPWRAKLRGCRRFRWRGNLFYREGQFARAAVQYEAALAWYEYTFPESSEGTAAVDAERVSALCNLAACQLQLRKYKDAEGSCDQALHMDAHCIKALFRRAVARRHQFLFEGAAADLRTLHSL------LTPPPPPLVREFRLLRAARAVYRKRKAQMSKSMF 486
            E+++ D+  + KL  C  FR  GNLF++ GQ+ RAA  Y  AL ++EY F +               L N AAC+L+     DA    +QAL +DA  +KAL+RRA A R +  F+ A  D+     L             LV+E  LL+A    Y+ R  Q+S +MF
Sbjct:   86 EQKLMDMTTQEKLGACDEFRVLGNLFFKHGQYQRAAFHYHKALVYFEYVFTDXXXXXXXXXXXXXXLLLNFAACRLKTMHLDDAVHHANQALEIDADNVKALYRRAQAYRLKDDFDLAQQDIARAIELSKEAGGAQSADAQLVQEKTLLQAKMLAYKLRTKQVSAAMF 253          
BLAST of mRNA_M-pyrifera_M_contig90357.20948.1 vs. uniprot
Match: H3GDM9_PHYRM (Uncharacterized protein n=1 Tax=Phytophthora ramorum TaxID=164328 RepID=H3GDM9_PHYRM)

HSP 1 Score: 87.0 bits (214), Expect = 2.260e-17
Identity = 58/168 (34.52%), Postives = 84/168 (50.00%), Query Frame = 1
Query:    1 ERRVFDLPWRAKLRGCRRFRWRGNLFYREGQFARAAVQYEAALAWYEYTFPESSEGTAAVDAERVSALCNLAACQLQLRKYKDAEGSCDQALHMDAHCIKALFRRAVARRHQFLFEGAAADLRTLHSLLTPPP------PPLVREFRLLRAARAVYRKRKAQMSKSMF 486
            E+++ D+    KL  C  FR  GNLF++ GQ+ RAA  Y  AL ++EY F +               L N AAC+L+     DA    +QAL +D   +KAL+RRA A R +  F+ A  D+     +           P LV+E +LL+A    Y+ R  Q+S +MF
Sbjct:  103 EQKLMDMTTLEKLAACDEFRVLGNLFFKHGQYQRAAFHYHKALVYFEYVFTDXXXXXXXXXXXXXXLLLNFAACRLKTMHLDDAVHHANQALEIDPDNVKALYRRAQAFRLKDEFDLAQKDVARAMEISKAAEENQAADPLLVQEKKLLQAKMLAYKLRTKQVSAAMF 270          
BLAST of mRNA_M-pyrifera_M_contig90357.20948.1 vs. uniprot
Match: A0A329SRI4_9STRA (Uncharacterized protein n=9 Tax=Phytophthora TaxID=4783 RepID=A0A329SRI4_9STRA)

HSP 1 Score: 87.0 bits (214), Expect = 3.310e-17
Identity = 57/168 (33.93%), Postives = 85/168 (50.60%), Query Frame = 1
Query:    1 ERRVFDLPWRAKLRGCRRFRWRGNLFYREGQFARAAVQYEAALAWYEYTFPESSEGTAAVDAERVSALCNLAACQLQLRKYKDAEGSCDQALHMDAHCIKALFRRAVARRHQFLFEGAAADLRTLHSLLTPPPPP------LVREFRLLRAARAVYRKRKAQMSKSMF 486
            E+++ D+  + KL  C  FR  GNLF++ GQ+ RAA  Y  AL ++EY F +               L N AAC+L+     DA    +QAL +D + +KAL+RRA A R +  F+ A  D+     L             L++E +LL+A    Y+ R  Q+S +MF
Sbjct:  107 EQKLMDMTTQEKLAACDEFRVLGNLFFKHGQYQRAAFHYHKALVYFEYVFTDXXXXXXXXXXXXXXLLLNFAACRLKTMHLDDAVHHANQALEIDPNNVKALYRRAQAYRLKDEFDLAQKDIERAVELSKAAEATQSVDSLLIQEKKLLQAKVLAYKLRTKQVSAAMF 274          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig90357.20948.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8D0R5_CAFRO2.400e-3553.74Uncharacterized protein n=3 Tax=Cafeteria roenberg... [more]
A0A662XSD5_9STRA1.020e-2340.00Uncharacterized protein n=1 Tax=Nothophytophthora ... [more]
A0A662XGD2_9STRA1.790e-2338.69Uncharacterized protein n=1 Tax=Nothophytophthora ... [more]
A0A7S2RXA3_9STRA2.650e-2237.20Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
D0NSG6_PHYIT4.960e-2136.31Uncharacterized protein n=2 Tax=Phytophthora infes... [more]
A0A812Y143_SYMMI1.450e-1937.41FKBP4 protein n=1 Tax=Symbiodinium microadriaticum... [more]
A0A6A3FIC8_9STRA8.900e-1835.71Uncharacterized protein n=4 Tax=Phytophthora TaxID... [more]
G4ZJT9_PHYSP1.950e-1735.12Uncharacterized protein n=1 Tax=Phytophthora sojae... [more]
H3GDM9_PHYRM2.260e-1734.52Uncharacterized protein n=1 Tax=Phytophthora ramor... [more]
A0A329SRI4_9STRA3.310e-1733.93Uncharacterized protein n=9 Tax=Phytophthora TaxID... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig90357contigM-pyrifera_M_contig90357:34..543 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score105.5
Seed ortholog evalue2.4e-20
Seed eggNOG ortholog115417.EPrPW00000015889
KEGG koko:K09571,ko:K20412
KEGG Pathwayko04915,map04915
Hectar predicted targeting categoryother localisation
GOsGO:0000413,GO:0003674,GO:0003755,GO:0003824,GO:0005488,GO:0005515,GO:0005516,GO:0005575,GO:0005623,GO:0005886,GO:0006464,GO:0006807,GO:0007275,GO:0008150,GO:0008152,GO:0009987,GO:0016020,GO:0016853,GO:0016859,GO:0018193,GO:0018208,GO:0019538,GO:0032501,GO:0032502,GO:0036211,GO:0043170,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044464,GO:0048366,GO:0048367,GO:0048731,GO:0048827,GO:0048856,GO:0071704,GO:0071944,GO:0099402,GO:0140096,GO:1901564
EggNOG free text desc.TPR domain-containing protein. Source PGD
EggNOG OGs1MEQW@121069,COG0545@1,KOG0543@2759
EC5.2.1.8
COG Functional cat.O
Best tax levelPythiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03110,ko04090
Exons1
Model size510
Cds size510
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816815.3645196-CDS-M-pyrifera_M_contig90357:33..5431622816815.3645196-CDS-M-pyrifera_M_contig90357:33..543Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig90357 34..543 +
1692278194.822119-CDS-M-pyrifera_M_contig90357:33..5431692278194.822119-CDS-M-pyrifera_M_contig90357:33..543Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig90357 34..543 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig90357.20948.1prot_M-pyrifera_M_contig90357.20948.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig90357 34..543 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig90357.20948.1

>prot_M-pyrifera_M_contig90357.20948.1 ID=prot_M-pyrifera_M_contig90357.20948.1|Name=mRNA_M-pyrifera_M_contig90357.20948.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=170bp
ERRVFDLPWRAKLRGCRRFRWRGNLFYREGQFARAAVQYEAALAWYEYTF
PESSEGTAAVDAERVSALCNLAACQLQLRKYKDAEGSCDQALHMDAHCIK
ALFRRAVARRHQFLFEGAAADLRTLHSLLTPPPPPLVREFRLLRAARAVY
RKRKAQMSKSMFQGEEEGDD
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mRNA from alignment at M-pyrifera_M_contig90357:34..543+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig90357.20948.1 ID=mRNA_M-pyrifera_M_contig90357.20948.1|Name=mRNA_M-pyrifera_M_contig90357.20948.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=510bp|location=Sequence derived from alignment at M-pyrifera_M_contig90357:34..543+ (Macrocystis pyrifera P11B4 male)
GAGAGACGTGTGTTTGATTTACCTTGGAGGGCCAAATTGCGTGGATGTCG ACGATTTCGATGGCGAGGCAACCTTTTTTATCGTGAAGGACAGTTTGCAC GTGCTGCGGTGCAATATGAAGCCGCTCTTGCATGGTATGAGTATACGTTT CCAGAAAGTAGTGAAGGAACTGCTGCAGTGGATGCAGAACGGGTGAGTGC ATTGTGTAATCTTGCTGCCTGTCAATTACAACTGCGAAAGTATAAAGATG CAGAAGGAAGTTGCGATCAAGCACTTCACATGGATGCACACTGTATCAAA GCATTGTTTCGTCGAGCTGTGGCACGAAGACATCAATTTTTGTTTGAAGG AGCAGCTGCAGACCTTCGCACCCTTCACTCTCTTCTCACACCTCCTCCAC CACCACTCGTGCGTGAGTTTCGACTCTTACGAGCTGCACGTGCGGTGTAT CGCAAGAGGAAAGCACAGATGAGCAAGTCTATGTTTCAAGGGGAGGAGGA AGGAGACGAT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig90357:34..543+

>mRNA_M-pyrifera_M_contig90357.20948.1 ID=mRNA_M-pyrifera_M_contig90357.20948.1|Name=mRNA_M-pyrifera_M_contig90357.20948.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1020bp|location=Sequence derived from alignment at M-pyrifera_M_contig90357:34..543+ (Macrocystis pyrifera P11B4 male)
GAGAGACGTGTGTTTGATTTACCTTGGAGGGCCAAATTGCGTGGATGTCG
ACGATTTCGATGGCGAGGCAACCTTTTTTATCGTGAAGGACAGTTTGCAC
GTGCTGCGGTGCAATATGAAGCCGCTCTTGCATGGTATGAGTATACGTTT
CCAGAAAGTAGTGAAGGAACTGCTGCAGTGGATGCAGAACGGGTGAGTGC
ATTGTGTAATCTTGCTGCCTGTCAATTACAACTGCGAAAGTATAAAGATG
CAGAAGGAAGTTGCGATCAAGCACTTCACATGGATGCACACTGTATCAAA
GCATTGTTTCGTCGAGCTGTGGCACGAAGACATCAATTTTTGTTTGAAGG
AGCAGCTGCAGACCTTCGCACCCTTCACTCTCTTCTCACACCTCCTCCAC
CACCACTCGTGCGTGAGTTTCGACTCTTACGAGCTGCACGTGCGGTGTAT
CGCAAGAGGAAAGCACAGATGAGCAAGTCTATGTTTCAAGGGGAGGAGGA
AGGAGACGATGAGAGACGTGTGTTTGATTTACCTTGGAGGGCCAAATTGC
GTGGATGTCGACGATTTCGATGGCGAGGCAACCTTTTTTATCGTGAAGGA
CAGTTTGCACGTGCTGCGGTGCAATATGAAGCCGCTCTTGCATGGTATGA
GTATACGTTTCCAGAAAGTAGTGAAGGAACTGCTGCAGTGGATGCAGAAC
GGGTGAGTGCATTGTGTAATCTTGCTGCCTGTCAATTACAACTGCGAAAG
TATAAAGATGCAGAAGGAAGTTGCGATCAAGCACTTCACATGGATGCACA
CTGTATCAAAGCATTGTTTCGTCGAGCTGTGGCACGAAGACATCAATTTT
TGTTTGAAGGAGCAGCTGCAGACCTTCGCACCCTTCACTCTCTTCTCACA
CCTCCTCCACCACCACTCGTGCGTGAGTTTCGACTCTTACGAGCTGCACG
TGCGGTGTATCGCAAGAGGAAAGCACAGATGAGCAAGTCTATGTTTCAAG
GGGAGGAGGAAGGAGACGAT
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