mRNA_M-pyrifera_M_contig8818.20481.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig8818.20481.1
Unique NamemRNA_M-pyrifera_M_contig8818.20481.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig8818.20481.1 vs. uniprot
Match: D8LL70_ECTSI (DUF4524 domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LL70_ECTSI)

HSP 1 Score: 184 bits (466), Expect = 9.250e-47
Identity = 196/507 (38.66%), Postives = 234/507 (46.15%), Query Frame = 1
Query:    1 VVVHADGTEVLLEGDFVRLSNGAVADAAXXXXXXXXXXXXXXXXXXXXXETCTSVSLGDIVARALALRRQFAASSVAVVAAETETAADAGRLPASVRYKIRTSKVQIAPAGNYSRLSTPAAXXXXXXXXXXPRAEAXXXXXXXXXXXARDGIGVAGPGSAPTTAKRSAGRKPHEFSAEVVERAEHGGASFVAFADGRARGAFADRTIVTLGAPGVDRGYSGCQRGDPGYXXXXXVHRGYPGVGFPLAGVFSEPEEGD--------REIECVRPDGTVVRLSERSLGHRFRFGTSCGGGDLAKATAATRPHGGNADHPIDITPYVVAVRRFADWALADPDERRARARRGADGRLAAAAEAERNRRFVALQ-----RLVKPPPL------------PTETTANGDAAGENRYSLGEFKKSSTERLLSCPVGGGDRGTPRAGEGWSSSEGN-KENVR---------CAEEHAG---WGSNEA-RERNALVNRLLEANREVLIGREALRRF 1404
            VVVHADG+EV LEGDFVRL     A                           TS+SLG+I +RA+ALR+QF   S    AA    A   G     V  KIR S V + P    S LS+P++           R  A             +G G    GS+ +  + +AG      S  VVE  E GGASFVAFADGRARG FADRTIV LGAP   R   G + G  G XXXXX                               RE+EC+R DGTV+RL+ RSL  R  FG   GGG++        P GG+      + PYVVAV RFADWA   P +RRA A R A      AAEAERNRRFV LQ     R V PPPL            P + T +G AAG  R      ++S  E   S   G   R     GE   + +G  KEN R           EE +G   WG  +  R+RN+LV  LLEANR+VL+G    R+F
Sbjct:  402 VVVHADGSEVWLEGDFVRLVRPPAAAGVPAMTAAAAEAGGGW----------TSMSLGNIASRAIALRQQFLQCSGNPAAAAAAAAGRRGPGEPQVTVKIRPSLVVVQPP---SALSSPSSSSSPVEAVV--RNAAAPRPTNKPYAPPEEG-GARWLGSSSSRGRMAAG------SPSVVESVERGGASFVAFADGRARGTFADRTIVCLGAPFFYRPLRGSRCGVGGXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXREVECIRADGTVLRLTRRSLDRRGSFGGRSGGGNI--------PAGGDCGVGA-LRPYVVAVLRFADWAATHPADRRAAAGREAAXXXXXAAEAERNRRFVDLQLLAAKRSVPPPPLLCYHRGGLDPREPYDGTPSG-AAGSRRGG----RRSGDE--ASASPGLRRRAVGACGE--ENDDGQYKENNRPRRPSNRVDVLEEGSGVSRWGGGDGGRQRNSLVAGLLEANRKVLMGEHMSRKF 868          
BLAST of mRNA_M-pyrifera_M_contig8818.20481.1 vs. uniprot
Match: A0A6H5KVD6_9PHAE (DUF4524 domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KVD6_9PHAE)

HSP 1 Score: 171 bits (432), Expect = 3.850e-42
Identity = 175/452 (38.72%), Postives = 213/452 (47.12%), Query Frame = 1
Query:  157 TSVSLGDIVARALALRRQFAASSVAVVAAETETAADAGRLPASVRYKIRTSKVQIAPAGNYSRLSTPAAXXXXXXXXXXPRAEAXXXXXXXXXXXARDGIGVAGPGSAPTTAKRSAGRKPHEFSAEVVERAEHGGASFVAFADGRARGAFADRTIVTLGAPGVDRGYSGCQRGDPGYXXXXXVHRG--YPGVGFPLAGVFS------------EPEEGDREIECVRPDGTVVRLSERSLGHRFRFGTSCGGGDLAKATAATRPHGGNADHPIDITPYVVAVRRFADWALADPDERRARARRGADGRLAAAAEAERNRRFVALQ-----RLVKPPPL------------PTETTANGDAAGENRYSLGEFKKSSTERLLSCPVG-GGDRGTPRAGEGWSSSEGNKENVRCAEEHAG---WGSNEA-RERNALVNRLLEANREVLIGREALRRF 1404
            TS+SLG I +RA+ALR+QF   S   +AA    A   G        KIR  +V + P    + LS+P++            A             A     +  P S   TA  S           VVE  E GGASFVAFADGRARGAFADRTIV+LGAP   R   G + G  G XXXXX  RG  Y G G P     +            E EE DREIEC+R DGTV+RL+  SL  R  FG   GGG+         P  GN      + PYVVAV RFADWA   P +RRA A R A G    AAEAERNRRFV LQ     R V PPPL            P + + +G A           + +++  L    VG GG+       +  S        V   E  +G   WG  +  R+RN+LV  LLEANR+VL+G +  R+F
Sbjct:  601 TSMSLGAITSRAIALRQQFLRCSG--IAATAAAAGIRGAGDHRDIVKIRPPRVVVQPP---AALSSPSSSSSPVEAVAGNAAAPRPTTKPYAPPEAGGARWLGSPSSRGGTAAGSP---------SVVELVERGGASFVAFADGRARGAFADRTIVSLGAPVFYRPLRGSRCG--GXXXXXXXXRGISYYGSGGPKGVATAVVRPGCSDVDEEEKEEEDREIECIRADGTVLRLTRHSLDRRGSFGGRSGGGNS--------PASGNCGVGA-LRPYVVAVLRFADWAATHPADRRAAAGREAAGXXXXAAEAERNRRFVDLQLLAAKRSVAPPPLLCYHRGGPDQRQPYDGSPSGAAGSRRGGRRSGDEANASPGLWRRAVGVGGEENNDDQDKENSRPRRPSNRVDVLEGSSGVSKWGGGDGGRQRNSLVAGLLEANRKVLMGEDLSRKF 1027          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig8818.20481.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 2
Match NameE-valueIdentityDescription
D8LL70_ECTSI9.250e-4738.66DUF4524 domain-containing protein n=1 Tax=Ectocarp... [more]
A0A6H5KVD6_9PHAE3.850e-4238.72DUF4524 domain-containing protein n=1 Tax=Ectocarp... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig8818contigM-pyrifera_M_contig8818:9516..11543 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Hectar predicted targeting categoryother localisation
Ec32 ortholog descriptionDomain of unknown function DUF4524
Ec32 orthologEc-24_002720.1
Exons6
Model size1434
Cds size1434
Stop1
Start0
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig8818.20481.1prot_M-pyrifera_M_contig8818.20481.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig8818 9516..11543 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816791.2879653-CDS-M-pyrifera_M_contig8818:9515..100901622816791.2879653-CDS-M-pyrifera_M_contig8818:9515..10090Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8818 9516..10090 -
1692278180.5031102-CDS-M-pyrifera_M_contig8818:9515..100901692278180.5031102-CDS-M-pyrifera_M_contig8818:9515..10090Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8818 9516..10090 -
1622816791.2989955-CDS-M-pyrifera_M_contig8818:10150..103701622816791.2989955-CDS-M-pyrifera_M_contig8818:10150..10370Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8818 10151..10370 -
1692278180.5139043-CDS-M-pyrifera_M_contig8818:10150..103701692278180.5139043-CDS-M-pyrifera_M_contig8818:10150..10370Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8818 10151..10370 -
1622816791.3108733-CDS-M-pyrifera_M_contig8818:10505..108141622816791.3108733-CDS-M-pyrifera_M_contig8818:10505..10814Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8818 10506..10814 -
1692278180.5235343-CDS-M-pyrifera_M_contig8818:10505..108141692278180.5235343-CDS-M-pyrifera_M_contig8818:10505..10814Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8818 10506..10814 -
1622816791.3226383-CDS-M-pyrifera_M_contig8818:11000..112011622816791.3226383-CDS-M-pyrifera_M_contig8818:11000..11201Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8818 11001..11201 -
1692278180.5330665-CDS-M-pyrifera_M_contig8818:11000..112011692278180.5330665-CDS-M-pyrifera_M_contig8818:11000..11201Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8818 11001..11201 -
1622816791.335561-CDS-M-pyrifera_M_contig8818:11228..113001622816791.335561-CDS-M-pyrifera_M_contig8818:11228..11300Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8818 11229..11300 -
1692278180.5424688-CDS-M-pyrifera_M_contig8818:11228..113001692278180.5424688-CDS-M-pyrifera_M_contig8818:11228..11300Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8818 11229..11300 -
1622816791.3492355-CDS-M-pyrifera_M_contig8818:11486..115431622816791.3492355-CDS-M-pyrifera_M_contig8818:11486..11543Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8818 11487..11543 -
1692278180.5518095-CDS-M-pyrifera_M_contig8818:11486..115431692278180.5518095-CDS-M-pyrifera_M_contig8818:11486..11543Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8818 11487..11543 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig8818.20481.1

>prot_M-pyrifera_M_contig8818.20481.1 ID=prot_M-pyrifera_M_contig8818.20481.1|Name=mRNA_M-pyrifera_M_contig8818.20481.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=478bp
VVVHADGTEVLLEGDFVRLSNGAVADAAAASAAAAVAAAAATGGRGGGGE
TCTSVSLGDIVARALALRRQFAASSVAVVAAETETAADAGRLPASVRYKI
RTSKVQIAPAGNYSRLSTPAAAAAAAATTTTPRAEAAATAAATTATTARD
GIGVAGPGSAPTTAKRSAGRKPHEFSAEVVERAEHGGASFVAFADGRARG
AFADRTIVTLGAPGVDRGYSGCQRGDPGYMGCMGVHRGYPGVGFPLAGVF
SEPEEGDREIECVRPDGTVVRLSERSLGHRFRFGTSCGGGDLAKATAATR
PHGGNADHPIDITPYVVAVRRFADWALADPDERRARARRGADGRLAAAAE
AERNRRFVALQRLVKPPPLPTETTANGDAAGENRYSLGEFKKSSTERLLS
CPVGGGDRGTPRAGEGWSSSEGNKENVRCAEEHAGWGSNEARERNALVNR
LLEANREVLIGREALRRFGLKYDTWAV*
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mRNA from alignment at M-pyrifera_M_contig8818:9516..11543-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig8818.20481.1 ID=mRNA_M-pyrifera_M_contig8818.20481.1|Name=mRNA_M-pyrifera_M_contig8818.20481.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=2028bp|location=Sequence derived from alignment at M-pyrifera_M_contig8818:9516..11543- (Macrocystis pyrifera P11B4 male)
GTTGTCGTCCACGCGGACGGGACGGAAGTGCTCCTTGAAGGCGACTTCGT GCGCCTTGTTCGCCCTCAAGCACTCACTACCGCCACCTCAAAGGGGCCAC AGGAACGCCTGCTGCACATCTCAGCGGCAACAACTTTCCGCTTCTTCGTG ACGCGAAGGGTGCAGCCGCTGCCACCAGGGCACACTTCGTTTCCGCTTGT CGGCGGTAGCAACGCTATCGGCGCTAACGCGAGCGGTAGCAAGTCAAACG GCGCCGTAGCTGACGCCGCTGCAGCTTCTGCCGCCGCCGCCGTTGCCGCT GCTGCTGCTACTGGCGTGACTCGGGGCAAGGAAGGGAGGGAGGGGCGGGG CGGGGGGGGAGAGACGTGCACAAGCGTTTCTCTCGGCGATATCGTCGCTC GGGCGCTAGCCTTGCGGCGGCAGTTCGCGGCGAGCAGTGTGGCGGTGGTA GCGGCTGAGACTGAGACCGCTGCTGATGCTGGGCGGCTGCCTGCGAGTGT TAGGTACAAAATACGCACCAGTAAGGTTCAGATCGCGCCTGCTGTGTTCG GGTCGAGGCTGGGACCAGCAGCTGCGGTGGCGGACGGGGTGGCCAACGCC GCTCGACTCTCCTCGAACTCCATGGCAACGGCAGCAACACCAACAACAGT GACGATGACACCGAGAACGGTAACAACAAACACAACAGTGTCAACAGCGG CAACAGCGGCAAAAGGTCGGCGACTGCAGGGAAATTATTCCCGGCTCAGT ACACCGGCAGCAGCAGCAGCAGCAGCAGCAACAACAACAACGCCGAGGGC CGAAGCAGCGGCAACAGCAGCAGCAACAACAGCAACAACGGCCAGGGATG GAATAGGAGTAGCAGGACCAGGATCAGCACCAACAACAGCCAAACGAAGC GCGGGAAGAAAGCCCCACGAGTTTTCCGCAGAGGTGGTAGAACGGGCGGA GCACGGCGGGGCGTCCTTCGTGGCGTTCGCGGACGGCCGAGCCCGCGGCG CATTCGCCGACCGCACCATCGTCACCCTCGGCGCGCCTGTTACCCTCAAG CCTGCGCCCTGTCTACGTCACCGCCCATCGTTGCCTCACCGCTGGGAACG GCGGCCTGCTGGCGGGAGAAGGAGGAAAGAGGAGTTGGCAAGCAGCACGG CGGCATCGACCCCGGACTACCAGGGTGTTGATCGGGGCTACTCCGGCTGC CAGCGCGGTGACCCGGGCTACATGGGCTGCATGGGTGTTCACCGAGGCTA CCCGGGTGTTGGTTTTCCCCTCGCAGGCGTGTTTTCTGAGCCGGAGGAAG GGGACCGGGAGATCGAGTGCGTACGTCCGGACGGCACCGTGGTTCGGCTC AGCGAACGCTCTCTCGGTCACCGGTTCCGCTTCGGCACCTCCTGTGGTTT CCGCACAGGAGGTTTAGACACCGGTGGCGACTCCTGTGGCGGCTTCGGCA GAGGTGGGGGTGGTGACTTGGCAAAGGCGACGGCGGCAACGAGACCACAT GGTGGGAACGCCGACCACCCCATCGATATCACGCCTTACGTTGTCGCGGT CCGCCGGTTCGCGGACTGGGCGCTCGCCGACCCGGACGAGCGGCGGGCGC GAGCGAGGCGTGGGGCGGACGGGAGGCTGGCAGCCGCGGCCGAGGCGGAG CGTAACCGTCGCTTTGTTGCGCTCCAGCGCCTCGTGAAACCACCGCCCTT GCCGACAGAAACAACGGCTAACGGCGATGCCGCTGGTGAGAACAGGTACT CATTGGGAGAGTTCAAAAAGAGTTCCACGGAAAGGCTTTTGTCGTGTCCG GTGGGGGGGGGTGACAGGGGGACACCACGAGCCGGGGAAGGGTGGTCGTC GAGCGAAGGTAATAAGGAAAATGTGCGTTGCGCCGAGGAGCATGCTGGGT GGGGTTCGAACGAAGCCCGCGAGAGGAACGCTCTAGTGAACCGGCTGCTC GAGGCCAATCGCGAGGTCTTGATCGGCCGGGAGGCTTTGCGAAGGTTCGG CCTGAAATACGATACATGGGCGGTTTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig8818:9516..11543-

>mRNA_M-pyrifera_M_contig8818.20481.1 ID=mRNA_M-pyrifera_M_contig8818.20481.1|Name=mRNA_M-pyrifera_M_contig8818.20481.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=2868bp|location=Sequence derived from alignment at M-pyrifera_M_contig8818:9516..11543- (Macrocystis pyrifera P11B4 male)
GTTGTCGTCCACGCGGACGGGACGGAAGTGCTCCTTGAAGGCGACTTCGT
GCGCCTTGTTGTCGTCCACGCGGACGGGACGGAAGTGCTCCTTGAAGGCG
ACTTCGTGCGCCTTTCAAACGGCGCCGTAGCTGACGCCGCTGCAGCTTCT
GCCGCCGCCGCCGTTGCCGCTGCTGCTGCTACTGGCTCAAACGGCGCCGT
AGCTGACGCCGCTGCAGCTTCTGCCGCCGCCGCCGTTGCCGCTGCTGCTG
CTACTGGCGGGCGGGGCGGGGGGGGAGAGACGTGCACAAGCGTTTCTCTC
GGCGATATCGTCGCTCGGGCGCTAGCCTTGCGGCGGCAGTTCGCGGCGAG
CAGTGTGGCGGTGGTAGCGGCTGAGACTGAGACCGCTGCTGATGCTGGGC
GGCTGCCTGCGAGTGTTAGGTACAAAATACGCACCAGTAAGGTTCAGATC
GCGCCTGCTGGGCGGGGCGGGGGGGGAGAGACGTGCACAAGCGTTTCTCT
CGGCGATATCGTCGCTCGGGCGCTAGCCTTGCGGCGGCAGTTCGCGGCGA
GCAGTGTGGCGGTGGTAGCGGCTGAGACTGAGACCGCTGCTGATGCTGGG
CGGCTGCCTGCGAGTGTTAGGTACAAAATACGCACCAGTAAGGTTCAGAT
CGCGCCTGCTGGAAATTATTCCCGGCTCAGTACACCGGCAGCAGCAGCAG
CAGCAGCAGCAACAACAACAACGCCGAGGGCCGAAGCAGCGGCAACAGCA
GCAGCAACAACAGCAACAACGGCCAGGGATGGAATAGGAGTAGCAGGACC
AGGATCAGCACCAACAACAGCCAAACGAAGCGCGGGAAGAAAGCCCCACG
AGTTTTCCGCAGAGGTGGTAGAACGGGCGGAGCACGGCGGGGCGTCCTTC
GTGGCGTTCGCGGACGGCCGAGCCCGCGGCGCATTCGCCGACCGCACCAT
CGTCACCCTCGGCGCGCCTGGAAATTATTCCCGGCTCAGTACACCGGCAG
CAGCAGCAGCAGCAGCAGCAACAACAACAACGCCGAGGGCCGAAGCAGCG
GCAACAGCAGCAGCAACAACAGCAACAACGGCCAGGGATGGAATAGGAGT
AGCAGGACCAGGATCAGCACCAACAACAGCCAAACGAAGCGCGGGAAGAA
AGCCCCACGAGTTTTCCGCAGAGGTGGTAGAACGGGCGGAGCACGGCGGG
GCGTCCTTCGTGGCGTTCGCGGACGGCCGAGCCCGCGGCGCATTCGCCGA
CCGCACCATCGTCACCCTCGGCGCGCCTGGTGTTGATCGGGGCTACTCCG
GCTGCCAGCGCGGTGACCCGGGCTACATGGGCTGCATGGGTGTTCACCGA
GGCTACCCGGGTGTTGGTTTTCCCCTCGCAGGCGTGTTTTCTGAGCCGGA
GGAAGGGGACCGGGAGATCGAGTGCGTACGTCCGGACGGCACCGTGGTTC
GGCTCAGCGAACGCTCTCTCGGTCACCGGTTCCGCTTCGGCACCTCCTGG
TGTTGATCGGGGCTACTCCGGCTGCCAGCGCGGTGACCCGGGCTACATGG
GCTGCATGGGTGTTCACCGAGGCTACCCGGGTGTTGGTTTTCCCCTCGCA
GGCGTGTTTTCTGAGCCGGAGGAAGGGGACCGGGAGATCGAGTGCGTACG
TCCGGACGGCACCGTGGTTCGGCTCAGCGAACGCTCTCTCGGTCACCGGT
TCCGCTTCGGCACCTCCTGTGGGGGTGGTGACTTGGCAAAGGCGACGGCG
GCAACGAGACCACATGGTGGGAACGCCGACCACCCCATCGATATCACGCC
TTACGTTGTCGCGGTCCGCCGGTTCGCGGACTGGGCGCTCGCCGACCCGG
ACGAGCGGCGGGCGCGAGCGAGGCGTGGGGCGGACGGGAGGCTGGCAGCC
GCGGCCGAGGCGGAGCGTAACCGTCGCTTTGTTGCGCTCCAGCGCCTCGT
GAAACCACCGCCCTTGCCGACAGAAACAACGGCTAACGGCGATGCCGCTG
GTGAGAACAGGTACTCATTGGGAGAGTTCAAAAAGAGTTCCACGGAAAGG
CTTTTGTCGTGTCCGGTGGGGGGGGGTGACAGGGGGACACCACGAGCCGG
GGAAGGGTGGTCGTCGAGCGAAGGTAATAAGGAAAATGTGCGTTGCGCCG
AGGAGCATGCTGGGTGGGGTTCGAACGAAGCCCGCGAGAGGAACGCTCTA
GTGAACCGGCTGCTCGAGGCCAATCGCGAGGTCTTGATCGGCCGGGAGGC
TTTGCGAAGGTTCGGCCTGAAATACGATACATGGGCGGTTTAAGTGGGGG
TGGTGACTTGGCAAAGGCGACGGCGGCAACGAGACCACATGGTGGGAACG
CCGACCACCCCATCGATATCACGCCTTACGTTGTCGCGGTCCGCCGGTTC
GCGGACTGGGCGCTCGCCGACCCGGACGAGCGGCGGGCGCGAGCGAGGCG
TGGGGCGGACGGGAGGCTGGCAGCCGCGGCCGAGGCGGAGCGTAACCGTC
GCTTTGTTGCGCTCCAGCGCCTCGTGAAACCACCGCCCTTGCCGACAGAA
ACAACGGCTAACGGCGATGCCGCTGGTGAGAACAGGTACTCATTGGGAGA
GTTCAAAAAGAGTTCCACGGAAAGGCTTTTGTCGTGTCCGGTGGGGGGGG
GTGACAGGGGGACACCACGAGCCGGGGAAGGGTGGTCGTCGAGCGAAGGT
AATAAGGAAAATGTGCGTTGCGCCGAGGAGCATGCTGGGTGGGGTTCGAA
CGAAGCCCGCGAGAGGAACGCTCTAGTGAACCGGCTGCTCGAGGCCAATC
GCGAGGTCTTGATCGGCCGGGAGGCTTTGCGAAGGTTCGGCCTGAAATAC
GATACATGGGCGGTTTAA
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