mRNA_M-pyrifera_M_contig87657.20361.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig87657.20361.1
Unique NamemRNA_M-pyrifera_M_contig87657.20361.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig87657.20361.1 vs. uniprot
Match: A0A1Q9NGH5_HEILC (Monomeric sarcosine oxidase n=1 Tax=Heimdallarchaeota archaeon (strain LC_2) TaxID=1841597 RepID=A0A1Q9NGH5_HEILC)

HSP 1 Score: 127 bits (318), Expect = 2.790e-32
Identity = 66/144 (45.83%), Postives = 94/144 (65.28%), Query Frame = 1
Query:   16 VIVVGGGVIGSAIAFHLAKGGDEVVLVEQFDSLHRRGSSGGESRIIRRTYPSTLYARLMVEAYDAWKVLEEETSTRLVHRCGGVDILPGNSSLLAPLVDANESCGVEAERLDRERLHERF-GLEVPDTYVGFVEENAGVLNANR 444
            VIV+G G +GSA  ++LAK G +V+L+EQF+ LH +GSS GESRIIRR YP   +A LM+ AYD W  LE ET   ++ + GG+D+   ++  L  L +A +S  +  E+LD E L  RF  L VP+ Y G  + +AG++NA +
Sbjct:    6 VIVIGAGAMGSATTYYLAKSGKKVLLLEQFEFLHPKGSSHGESRIIRRAYPEKHFADLMLHAYDLWNELERETMREIIKKTGGLDMGHKDNKDLNSLEEACKSLDINYEKLDAENLRIRFPNLIVPEDYAGIYQSDAGIINATQ 149          
BLAST of mRNA_M-pyrifera_M_contig87657.20361.1 vs. uniprot
Match: A0A1Q9NGD1_HEILC (Monomeric sarcosine oxidase (Fragment) n=1 Tax=Heimdallarchaeota archaeon (strain LC_2) TaxID=1841597 RepID=A0A1Q9NGD1_HEILC)

HSP 1 Score: 119 bits (299), Expect = 3.470e-31
Identity = 62/138 (44.93%), Postives = 89/138 (64.49%), Query Frame = 1
Query:   34 GVIGSAIAFHLAKGGDEVVLVEQFDSLHRRGSSGGESRIIRRTYPSTLYARLMVEAYDAWKVLEEETSTRLVHRCGGVDILPGNSSLLAPLVDANESCGVEAERLDRERLHERF-GLEVPDTYVGFVEENAGVLNANR 444
            G +GSA  ++LAK G +V+L+EQF+ LH +GSS GESRIIRR YP   +A LM+ AYD W  LE ET   ++ + GG+D+   ++  L  L +A +S  +  E+LD E L  RF  L VP+ Y G  + +AG++NA +
Sbjct:    2 GAMGSATTYYLAKSGKKVLLLEQFEFLHPKGSSHGESRIIRRAYPEKHFADLMLHAYDLWNELERETMREIIKKTGGLDMGHKDNKDLNSLEEACKSLDINYEKLDAENLRIRFPNLIVPEDYAGIYQSDAGIINATQ 139          
BLAST of mRNA_M-pyrifera_M_contig87657.20361.1 vs. uniprot
Match: A0A8J5AVH7_9DIPT (Sarcosine oxidase n=2 Tax=Bradysia TaxID=35571 RepID=A0A8J5AVH7_9DIPT)

HSP 1 Score: 122 bits (306), Expect = 1.840e-30
Identity = 61/146 (41.78%), Postives = 97/146 (66.44%), Query Frame = 1
Query:    7 KMRVIVVGGGVIGSAIAFHLAKG-GDEVVLVEQFDSLHRRGSSGGESRIIRRTYPSTLYARLMVEAYDAWKVLEEETSTRLVHRCGGVDILPGNSSLLAPLVDANESCGVEAERLDRERLHERF-GLEVPDTYVGFVEENAGVLNA 438
            +  VI++GGGV+GSA A++L+K  G  V+L+EQ+D LHRRGSS G+SRIIR+TYP   Y  LM++AY+ W + E+E  TR+  + GG+DI   ++  +  L+ + E   V  + L   +++E+F  + +PD  +G    ++G+LNA
Sbjct:    5 RYEVIIIGGGVMGSAAAYYLSKCLGKRVLLIEQYDLLHRRGSSHGDSRIIRKTYPQEHYTELMLDAYELWSLAEKEAETRVFTKTGGLDISDRDNRQIKQLLQSAEKYNVPVKVLTPSQVNEKFPSVTIPDDCIGVYSPDSGILNA 150          
BLAST of mRNA_M-pyrifera_M_contig87657.20361.1 vs. uniprot
Match: A0A1Y1IKN2_KLENI (Sarcosine oxidase n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1IKN2_KLENI)

HSP 1 Score: 115 bits (289), Expect = 7.720e-28
Identity = 65/149 (43.62%), Postives = 91/149 (61.07%), Query Frame = 1
Query:    1 DLKMRVIVVGGGVIGSAIAFHLAKGGDEVVLVEQFDSLHRRGSSGGESRIIRRTYPSTLYARLMVEAYDAWKVLEEETSTRLVHRCGGVDILPGNSSLLAPLVDANESCGVEAERLDRERLHERFG-LEVPDTYVGFVEENAGVLNANR 444
            D+   VIVVG G++GSA A+ L + G  V+L+EQFD LHRRGSS GESRIIRRTYP   Y ++M  +Y+ W+  ++E   R+  + GG+D    N++ +  L+ + E   VE E L      +RF  L +PD +V      AGVLNA +
Sbjct:    3 DITHDVIVVGAGIMGSATAWELTRRGRTVLLLEQFDFLHRRGSSHGESRIIRRTYPQDYYTKMMELSYNLWEEAQQEAGVRVYTQTGGLDFGRRNNAEVQGLIRSCEVHKVEHEVLTAAAAMDRFPQLRLPDDHVAIYSPAAGVLNATK 151          
BLAST of mRNA_M-pyrifera_M_contig87657.20361.1 vs. uniprot
Match: A0A1S3CR44_CUCME (Sarcosine oxidase n=3 Tax=Cucumis TaxID=3655 RepID=A0A1S3CR44_CUCME)

HSP 1 Score: 115 bits (287), Expect = 1.370e-27
Identity = 62/150 (41.33%), Postives = 89/150 (59.33%), Query Frame = 1
Query:    1 DLKMRVIVVGGGVIGSAIAFHLAKGGDEVVLVEQFDSLHRRGSSGGESRIIRRTYPSTLYARLMVEAYDAWKVLEEETSTRLVHRCGGVDILPGNSSLLAPLVDANESCGVEAERLDRERLHERFG--LEVPDTYVGFVEENAGVLNANR 444
            D +  VIVVG GV+GS+ A+HLAK G+ V+L+EQFD LH RGSS GESR IR TYP   Y  L++E+Y+ W++ E E   ++ +    +DI P NS  L  +VD      +    LDR  L E++   +E+P  +V    +  GV+   +
Sbjct:    5 DTQFDVIVVGAGVMGSSTAYHLAKTGNRVLLLEQFDFLHHRGSSHGESRTIRATYPEDYYHDLVMESYELWRMAEAEIGFKVYYPAEQLDIGPSNSESLTAVVDTCLKHSIPHLVLDRGELMEKYSGRVEIPANWVAVCSKYGGVIKPTK 154          
BLAST of mRNA_M-pyrifera_M_contig87657.20361.1 vs. uniprot
Match: A0A7R9U0M9_9VIRI (Hypothetical protein n=1 Tax=Prasinoderma coloniale TaxID=156133 RepID=A0A7R9U0M9_9VIRI)

HSP 1 Score: 113 bits (282), Expect = 8.980e-27
Identity = 59/142 (41.55%), Postives = 85/142 (59.86%), Query Frame = 1
Query:   22 VVGGGVIGSAIAFHLAKGGDEVVLVEQFDSLHRRGSSGGESRIIRRTYPSTLYARLMVEAYDAWKVLEEETSTRLVHRCGGVDILPGNSSLLAPLVDANESCGVEAERLDRERLHERF-GLEVPDTYVGFVEENAGVLNANR 444
            VVGGG+ G  +A+  A+ G   +L+E+ D LHRRGSS GESRIIR TY    Y  +M +AY  W+  ++E  T +    GG+D  P   + +  ++ A E+ GV AER+D ER+  RF G  +PD +      +AG+LNA +
Sbjct:    1 VVGGGIAGVCVAYECARRGARTLLLERHDMLHRRGSSHGESRIIRPTYAEEHYTAMMRKAYPLWESAQQEARTSVYTPTGGLDFGPAEGAGMRRVLAAAEANGVRAERMDAERVSRRFPGFSIPDHWTALWNADAGILNATK 142          
BLAST of mRNA_M-pyrifera_M_contig87657.20361.1 vs. uniprot
Match: A0A540MP72_MALBA (Sarcosine oxidase n=1 Tax=Malus baccata TaxID=106549 RepID=A0A540MP72_MALBA)

HSP 1 Score: 112 bits (281), Expect = 9.850e-27
Identity = 57/148 (38.51%), Postives = 88/148 (59.46%), Query Frame = 1
Query:    7 KMRVIVVGGGVIGSAIAFHLAKGGDEVVLVEQFDSLHRRGSSGGESRIIRRTYPSTLYARLMVEAYDAWKVLEEETSTRLVHRCGGVDILPGNSSLLAPLVDANESCGVEAERLDRERLHERFG--LEVPDTYVGFVEENAGVLNANR 444
            +  VIVVG GV+GS+ A+  AK G + +L+EQFD LH RGSS GESR IR TYP   Y  L++E+Y  W+  E E    +  +   +D+ P N  +L  +V++     V    ++R++LH+ F   + +P+ +VG V E+ GV+   +
Sbjct:    7 EFDVIVVGAGVMGSSTAYQTAKRGHKTLLLEQFDFLHHRGSSHGESRTIRATYPEDYYTPLVLESYKLWQQAESEIGYNVYFKANQLDMAPANDKVLLAVVESCRKNSVAFSVMNRDQLHQEFSGLVMIPEDWVGVVTEHGGVIKPTK 154          
BLAST of mRNA_M-pyrifera_M_contig87657.20361.1 vs. uniprot
Match: A0A251TJ42_HELAN (Putative FAD/NAD(P)-binding domain-containing protein n=1 Tax=Helianthus annuus TaxID=4232 RepID=A0A251TJ42_HELAN)

HSP 1 Score: 110 bits (276), Expect = 5.420e-26
Identity = 57/146 (39.04%), Postives = 86/146 (58.90%), Query Frame = 1
Query:    7 KMRVIVVGGGVIGSAIAFHLAKGGDEVVLVEQFDSLHRRGSSGGESRIIRRTYPSTLYARLMVEAYDAWKVLEEETSTRLVHRCGGVDILPGNSSLLAPLVDANESCGVEAERLDRERLHERFGLEVPDTYVGFVEENAGVLNANR 444
            +  +I++G GV+GS+ A+  AK G + +L+EQFD LH RGSS GESR IR TYP   Y ++++E+   WK  E E   ++  +   +D+ P  SS L  ++D  E        LDRE+ +E F +E+PD ++  V E  GVL   +
Sbjct:    3 QFDIIIIGAGVMGSSAAYQAAKSGHKTLLLEQFDFLHYRGSSHGESRTIRATYPEPYYTQMVIESEKLWKETELEIGYKVYFKTQQLDLGPKESSSLVAVIDNCEKQLNRYRILDREQANECFSVEIPDDWIAVVTELGGVLKPTK 148          
BLAST of mRNA_M-pyrifera_M_contig87657.20361.1 vs. uniprot
Match: UPI001900B029 (probable sarcosine oxidase n=1 Tax=Benincasa hispida TaxID=102211 RepID=UPI001900B029)

HSP 1 Score: 110 bits (274), Expect = 9.680e-26
Identity = 59/148 (39.86%), Postives = 87/148 (58.78%), Query Frame = 1
Query:    7 KMRVIVVGGGVIGSAIAFHLAKGGDEVVLVEQFDSLHRRGSSGGESRIIRRTYPSTLYARLMVEAYDAWKVLEEETSTRLVHRCGGVDILPGNSSLLAPLVDANESCGVEAERLDRERLHERFG--LEVPDTYVGFVEENAGVLNANR 444
            +  VIVVG GV+GS+  +HLAK G+ V+++EQFD LH RGSS GESR IR TYP   Y  L++E+Y+ W++ E E   R+      +DI P +   LA +VD      +    LDR +L E++   +E+P  +V    +  GV+   +
Sbjct:    7 QFDVIVVGAGVMGSSTGYHLAKTGNRVLILEQFDFLHHRGSSHGESRTIRATYPEDYYHGLVMESYELWRMAEAEIGYRVYFPAEQLDIGPSDDKSLAAVVDTCRKHSIPHMVLDRGQLAEKYSGRVEIPSDWVAVWSKYGGVIKPTK 154          
BLAST of mRNA_M-pyrifera_M_contig87657.20361.1 vs. uniprot
Match: A0A6J1IIQ6_CUCMA (Sarcosine oxidase n=3 Tax=Cucurbita TaxID=3660 RepID=A0A6J1IIQ6_CUCMA)

HSP 1 Score: 109 bits (272), Expect = 1.870e-25
Identity = 60/145 (41.38%), Postives = 87/145 (60.00%), Query Frame = 1
Query:   16 VIVVGGGVIGSAIAFHLAKGGDEVVLVEQFDSLHRRGSSGGESRIIRRTYPSTLYARLMVEAYDAWKVLEEETSTRLVHRCGGVDILPGNSSLLAPLVDANESCGVEAERLDRERLHERFG--LEVPDTYVGFVEENAGVLNANR 444
            VIVVG GV+GS+ A+HLAK G++V+++EQFD LH RGSS GESR IR TYP   Y  L++E+Y+ W+  E E   R+      +DI   +   LA  VD  +   +    LDRE+L E++   +E+P  +V    +  GV+   +
Sbjct:   10 VIVVGAGVMGSSTAYHLAKTGNKVLILEQFDFLHHRGSSHGESRTIRATYPEDYYHSLVMESYELWRTAEAEIGYRVYFPAEQLDIGHSDDKSLAAAVDTCKKHSIPHLVLDREQLAEKYSGRVEIPADWVAVWSKYGGVIKPTK 154          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig87657.20361.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1Q9NGH5_HEILC2.790e-3245.83Monomeric sarcosine oxidase n=1 Tax=Heimdallarchae... [more]
A0A1Q9NGD1_HEILC3.470e-3144.93Monomeric sarcosine oxidase (Fragment) n=1 Tax=Hei... [more]
A0A8J5AVH7_9DIPT1.840e-3041.78Sarcosine oxidase n=2 Tax=Bradysia TaxID=35571 Rep... [more]
A0A1Y1IKN2_KLENI7.720e-2843.62Sarcosine oxidase n=1 Tax=Klebsormidium nitens Tax... [more]
A0A1S3CR44_CUCME1.370e-2741.33Sarcosine oxidase n=3 Tax=Cucumis TaxID=3655 RepID... [more]
A0A7R9U0M9_9VIRI8.980e-2741.55Hypothetical protein n=1 Tax=Prasinoderma colonial... [more]
A0A540MP72_MALBA9.850e-2738.51Sarcosine oxidase n=1 Tax=Malus baccata TaxID=1065... [more]
A0A251TJ42_HELAN5.420e-2639.04Putative FAD/NAD(P)-binding domain-containing prot... [more]
UPI001900B0299.680e-2639.86probable sarcosine oxidase n=1 Tax=Benincasa hispi... [more]
A0A6J1IIQ6_CUCMA1.870e-2541.38Sarcosine oxidase n=3 Tax=Cucurbita TaxID=3660 Rep... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig87657contigM-pyrifera_M_contig87657:1..444 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score112.1
Seed ortholog evalue2.2e-22
Seed eggNOG ortholog3750.XP_008347646.1
KEGG rclassRC00060,RC00083,RC00557
KEGG koko:K00306
KEGG ReactionR00610,R02204
KEGG Pathwayko00260,ko00310,ko01100,ko04146,map00260,map00310,map01100,map04146
Hectar predicted targeting categorysignal peptide
GOsGO:0003674,GO:0003824,GO:0008115,GO:0008150,GO:0008152,GO:0016491,GO:0016645,GO:0016647,GO:0055114
EggNOG free text desc.Sarcosine oxidase
EggNOG OGs37NAT@33090,3G7V3@35493,4JDVH@91835,COG0665@1,KOG2820@2759
EC1.5.3.1,1.5.3.7
COG Functional cat.E
Best tax levelfabids
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000
Exons1
Model size444
Cds size435
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816785.5854607-CDS-M-pyrifera_M_contig87657:0..4351622816785.5854607-CDS-M-pyrifera_M_contig87657:0..435Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87657 1..435 -
1692278177.3774977-CDS-M-pyrifera_M_contig87657:0..4351692278177.3774977-CDS-M-pyrifera_M_contig87657:0..435Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87657 1..435 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816785.5963595-UTR-M-pyrifera_M_contig87657:435..4441622816785.5963595-UTR-M-pyrifera_M_contig87657:435..444Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig87657 436..444 -
1692278177.3950758-UTR-M-pyrifera_M_contig87657:435..4441692278177.3950758-UTR-M-pyrifera_M_contig87657:435..444Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig87657 436..444 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig87657.20361.1prot_M-pyrifera_M_contig87657.20361.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig87657 1..435 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig87657.20361.1

>prot_M-pyrifera_M_contig87657.20361.1 ID=prot_M-pyrifera_M_contig87657.20361.1|Name=mRNA_M-pyrifera_M_contig87657.20361.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=145bp
MRVIVVGGGVIGSAIAFHLAKGGDEVVLVEQFDSLHRRGSSGGESRIIRR
TYPSTLYARLMVEAYDAWKVLEEETSTRLVHRCGGVDILPGNSSLLAPLV
DANESCGVEAERLDRERLHERFGLEVPDTYVGFVEENAGVLNANR
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mRNA from alignment at M-pyrifera_M_contig87657:1..444-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig87657.20361.1 ID=mRNA_M-pyrifera_M_contig87657.20361.1|Name=mRNA_M-pyrifera_M_contig87657.20361.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=444bp|location=Sequence derived from alignment at M-pyrifera_M_contig87657:1..444- (Macrocystis pyrifera P11B4 male)
GATCTGAAGATGAGGGTGATTGTTGTAGGTGGAGGAGTGATTGGGAGTGC AATTGCATTCCATCTTGCTAAGGGTGGTGATGAGGTTGTGTTGGTGGAGC AGTTTGATTCATTACATCGACGAGGATCTTCGGGAGGTGAGTCTCGCATC ATTCGGCGCACCTATCCGTCTACTCTATATGCTAGACTAATGGTGGAGGC TTACGATGCTTGGAAAGTGTTGGAAGAGGAGACATCTACCCGCTTGGTGC ATCGTTGTGGCGGTGTAGATATTTTGCCTGGCAATTCCTCGTTACTCGCA CCTCTTGTGGATGCAAATGAGTCTTGTGGAGTGGAAGCAGAACGTTTAGA TCGAGAACGTTTGCATGAACGGTTTGGTTTGGAGGTTCCAGACACTTATG TAGGCTTTGTAGAAGAAAATGCTGGTGTTTTGAATGCAAATCGT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig87657:1..444-

>mRNA_M-pyrifera_M_contig87657.20361.1 ID=mRNA_M-pyrifera_M_contig87657.20361.1|Name=mRNA_M-pyrifera_M_contig87657.20361.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=870bp|location=Sequence derived from alignment at M-pyrifera_M_contig87657:1..444- (Macrocystis pyrifera P11B4 male)
ATGAGGGTGATTGTTGTAGGTGGAGGAGTGATTGGGAGTGCAATTGCATT
CCATCTTGCTAAGGGTGGTGATGAGGTTGTGTTGGTGGAGCAGTTTGATT
CATTACATCGACGAGGATCTTCGGGAGGTGAGTCTCGCATCATTCGGCGC
ACCTATCCGTCTACTCTATATGCTAGACTAATGGTGGAGGCTTACGATGC
TTGGAAAGTGTTGGAAGAGGAGACATCTACCCGCTTGGTGCATCGTTGTG
GCGGTGTAGATATTTTGCCTGGCAATTCCTCGTTACTCGCACCTCTTGTG
GATGCAAATGAGTCTTGTGGAGTGGAAGCAGAACGTTTAGATCGAGAACG
TTTGCATGAACGGTTTGGTTTGGAGGTTCCAGACACTTATGTAGGCTTTG
TAGAAGAAAATGCTGGTGTTTTGAATGCAAATCGTATGAGGGTGATTGTT
GTAGGTGGAGGAGTGATTGGGAGTGCAATTGCATTCCATCTTGCTAAGGG
TGGTGATGAGGTTGTGTTGGTGGAGCAGTTTGATTCATTACATCGACGAG
GATCTTCGGGAGGTGAGTCTCGCATCATTCGGCGCACCTATCCGTCTACT
CTATATGCTAGACTAATGGTGGAGGCTTACGATGCTTGGAAAGTGTTGGA
AGAGGAGACATCTACCCGCTTGGTGCATCGTTGTGGCGGTGTAGATATTT
TGCCTGGCAATTCCTCGTTACTCGCACCTCTTGTGGATGCAAATGAGTCT
TGTGGAGTGGAAGCAGAACGTTTAGATCGAGAACGTTTGCATGAACGGTT
TGGTTTGGAGGTTCCAGACACTTATGTAGGCTTTGTAGAAGAAAATGCTG
GTGTTTTGAATGCAAATCGT
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