mRNA_M-pyrifera_M_contig87100.20256.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig87100.20256.1
Unique NamemRNA_M-pyrifera_M_contig87100.20256.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig87100.20256.1 vs. uniprot
Match: A0A5A8CCE0_CAFRO (MPN domain-containing protein n=3 Tax=Sar TaxID=2698737 RepID=A0A5A8CCE0_CAFRO)

HSP 1 Score: 187 bits (476), Expect = 9.830e-56
Identity = 91/141 (64.54%), Postives = 111/141 (78.72%), Query Frame = 1
Query:    1 VRHAYSGVESGLARESNPMPIEIMGLMFGHLDPDHPNELVVTDTFPLPIEGSETTVVADKPEVNAFMSELTESLSKTRKDRLCGWYHSHPFPVGEFSNCFFSAVDVQTQLSWQMNFEAMG---DPFVGIVVDPLRSVAQGR 414
            ++HA +GVE G+AR S P+PIEIMGLM GH+DPD    LVVTD FPLP+EG+ET+VVAD P VN +M+EL+ES+   RK+RL GWYHSHPF V E+ N FFS VDVQTQ +WQ   E MG   DPF+GIV+DPLRS+A+GR
Sbjct:   49 LQHAQTGVERGMAR-SPPVPIEIMGLMIGHVDPDDTRTLVVTDAFPLPVEGTETSVVADNPRVNEYMAELSESIEAVRKERLIGWYHSHPFDVREYPNWFFSMVDVQTQNAWQHQSERMGSASDPFIGIVIDPLRSLAKGR 188          
BLAST of mRNA_M-pyrifera_M_contig87100.20256.1 vs. uniprot
Match: A0A7S1VJP8_9STRA (Hypothetical protein n=1 Tax=Grammatophora oceanica TaxID=210454 RepID=A0A7S1VJP8_9STRA)

HSP 1 Score: 155 bits (393), Expect = 2.290e-44
Identity = 76/135 (56.30%), Postives = 92/135 (68.15%), Query Frame = 1
Query:    7 HAYSGVESGLARESNPMPIEIMGLMFGHLDPDHPNELVVTDTFPLPIEGSETTVVADKPEVNAFMSELTESLSKTRKDRLCGWYHSHPFPVGEFSNCFFSAVDVQTQLSWQMNFEAMGDPFVGIVVDPLRSVAQG 411
            H  SGV+ G+ +  NP  IE+MG++ G  DPD P  LVVTD FPLPIEG ET VVAD   V   M +L E L  TR ++  GWYHSHPF +G+ S+CFFS  D+ TQL WQ   +  G+PFVGIVVDPLRS+ QG
Sbjct:    4 HCQSGVKKGIDKGGNP--IEVMGMLLGRPDPDTPKTLVVTDAFPLPIEGFETRVVADDANVVNHMIQLGECLEATRNEKFMGWYHSHPFDLGDHSHCFFSQTDLSTQLQWQRAEDPHGNPFVGIVVDPLRSIHQG 136          
BLAST of mRNA_M-pyrifera_M_contig87100.20256.1 vs. uniprot
Match: A0A6U3S5X1_9STRA (Hypothetical protein n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A6U3S5X1_9STRA)

HSP 1 Score: 157 bits (398), Expect = 5.140e-44
Identity = 74/134 (55.22%), Postives = 96/134 (71.64%), Query Frame = 1
Query:    7 HAYSGVESGLARESNPMPIEIMGLMFGHLDPDHPNELVVTDTFPLPIEGSETTVVADKPEVNAFMSELTESLSKTRKDRLCGWYHSHPFPVGEFSNCFFSAVDVQTQLSWQMNFEAMGDPFVGIVVDPLRSVAQ 408
            H +SGVE G+ +  NP  IE+MGL+ G  DPD P +LVVTD FP+PIEG ET VVAD  +   +M  L ESL +TRK+++ GWYHSHPF VG  S+C+ S  D+ TQL WQ + +  G+PF+ IVVDPLRS+A+
Sbjct:   65 HCHSGVEKGMKKGGNP--IEVMGLLLGRPDPDTPKQLVVTDAFPIPIEGFETRVVADDLDAQNYMISLGESLERTRKEKIMGWYHSHPFDVGTDSHCYLSQTDINTQLLWQRSEDPRGNPFLAIVVDPLRSLAK 196          
BLAST of mRNA_M-pyrifera_M_contig87100.20256.1 vs. uniprot
Match: A0A7S4N3G5_9STRA (Hypothetical protein (Fragment) n=2 Tax=Odontella aurita TaxID=265563 RepID=A0A7S4N3G5_9STRA)

HSP 1 Score: 155 bits (392), Expect = 5.740e-44
Identity = 75/134 (55.97%), Postives = 93/134 (69.40%), Query Frame = 1
Query:    7 HAYSGVESGLARESNPMPIEIMGLMFGHLDPDHPNELVVTDTFPLPIEGSETTVVADKPEVNAFMSELTESLSKTRKDRLCGWYHSHPFPVGEFSNCFFSAVDVQTQLSWQMNFEAMGDPFVGIVVDPLRSVAQ 408
            H +SGVE GL +  NP  IE+MGLM G  DPD P  LVVTD FPLPIEG ET V+AD  +V   M  L ES+ +TR+++  GWYHSHPF VG  S+CF S  D+ TQL WQ   +  G+PF+ +VVDPLRS+A+
Sbjct:   69 HCHSGVEKGLKKGGNP--IEVMGLMLGRPDPDTPTTLVVTDVFPLPIEGFETRVIADDEDVVNHMIALGESMERTRREKFMGWYHSHPFDVGIHSHCFLSQTDISTQLQWQRAEDPHGNPFLAVVVDPLRSLAK 200          
BLAST of mRNA_M-pyrifera_M_contig87100.20256.1 vs. uniprot
Match: A0A7S2LVQ6_9STRA (Hypothetical protein (Fragment) n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A7S2LVQ6_9STRA)

HSP 1 Score: 154 bits (390), Expect = 5.830e-44
Identity = 76/136 (55.88%), Postives = 93/136 (68.38%), Query Frame = 1
Query:    7 HAYSGVESGLARESNPMPIEIMGLMFGHLDPDHPNELVVTDTFPLPIEGSETTVVADKPEVNAFMSELTESLSKTRKDRLCGWYHSHPFPVGEFSNCFFSAVDVQTQLSWQMNFEAMGDPFVGIVVDPLRSVAQGR 414
            H +SGVE G+ +  NP  IE+MGL+ G  DP+ P  LVVTD FPLPIEG ET V+AD  +V   M  L ESL  TRK++  GWYHSHPF VG  S+CF S  D+ TQL WQ   +  G+PF+ IVVDPLRS+A+G 
Sbjct:   66 HCHSGVEKGVKKGGNP--IEVMGLLLGRPDPNTPKTLVVTDVFPLPIEGFETRVIADDGDVINHMIALGESLETTRKEKFMGWYHSHPFDVGIHSHCFLSQTDISTQLQWQRAEDPHGNPFLAIVVDPLRSLAKGH 199          
BLAST of mRNA_M-pyrifera_M_contig87100.20256.1 vs. uniprot
Match: K0RC45_THAOC (MPN domain-containing protein n=1 Tax=Thalassiosira oceanica TaxID=159749 RepID=K0RC45_THAOC)

HSP 1 Score: 155 bits (391), Expect = 2.690e-43
Identity = 73/136 (53.68%), Postives = 94/136 (69.12%), Query Frame = 1
Query:    7 HAYSGVESGLARESNPMPIEIMGLMFGHLDPDHPNELVVTDTFPLPIEGSETTVVADKPEVNAFMSELTESLSKTRKDRLCGWYHSHPFPVGEFSNCFFSAVDVQTQLSWQMNFEAMGDPFVGIVVDPLRSVAQGR 414
            H +SGVE G+ +  NP  IE+MGL+ G  DP  P  L+VTD FPLPIEG ET VVAD  +V   M  L ESL  TRK++  GWYHSHPF VG++SNC+ S  D+ TQ+ WQ + +  G+PF+ IV+DPLRS+ +G 
Sbjct:   54 HCHSGVEKGIKKGGNP--IEVMGLIHGRPDPTTPQTLIVTDVFPLPIEGFETRVVADDGDVVNHMIALGESLESTRKEKFMGWYHSHPFEVGQYSNCYMSQTDMSTQIQWQRSEDPHGNPFLAIVLDPLRSLVKGN 187          
BLAST of mRNA_M-pyrifera_M_contig87100.20256.1 vs. uniprot
Match: A0A2R5GXA2_9STRA (COP9 signalosome complex subunit 5 n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GXA2_9STRA)

HSP 1 Score: 155 bits (392), Expect = 2.920e-43
Identity = 76/136 (55.88%), Postives = 96/136 (70.59%), Query Frame = 1
Query:    7 HAYSGVESGLARESNPMPIEIMGLMFGHLDPDHPNELVVTDTFPLPIEGSETTVVADKPEVNAFMSELTESLSKTRKDRLCGWYHSHPFPVGEFSNCFFSAVDVQTQLSWQMNFEAMGDPFVGIVVDPLRSVAQGR 414
            HA SGVE G+   +   P+E+MGLM GHL P+ P  LVV+D F LPIEG+ET V+AD  EV  FM  L ESL KTR++R  GWYHSHPF V   S+CF S+ D+ TQL WQ   +  G+P++ IV+DPLRS+A+GR
Sbjct:    6 HAVSGVEKGM--NAGGRPVEVMGLMLGHLCPESPETLVVSDCFALPIEGAETRVLADDQEVINFMIGLGESLEKTRQERFMGWYHSHPFDVEVHSHCFMSSTDISTQLGWQRAEDRNGNPWLAIVIDPLRSLAKGR 139          
BLAST of mRNA_M-pyrifera_M_contig87100.20256.1 vs. uniprot
Match: B7FQK5_PHATC (COP9 SigNalosome subunit 5 n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=B7FQK5_PHATC)

HSP 1 Score: 152 bits (384), Expect = 2.880e-42
Identity = 73/131 (55.73%), Postives = 90/131 (68.70%), Query Frame = 1
Query:    7 HAYSGVESGLARESNPMPIEIMGLMFGHLDPDHPNELVVTDTFPLPIEGSETTVVADKPEVNAFMSELTESLSKTRKDRLCGWYHSHPFPVGEFSNCFFSAVDVQTQLSWQMNFEAMGDPFVGIVVDPLRS 399
            H  SGV+ G+A+  NP  IE+MG++ G  DPD P  LV+TD FPLPIEG ET V+AD   V   M  L ESL +TRK++  GWYHSHPF +G+ S+CF S  D+ TQL WQ   +  G+PFV IVVDPLRS
Sbjct:   75 HCQSGVQKGIAKGGNP--IEVMGMLLGRPDPDTPRTLVITDAFPLPIEGFETRVIADDENVVNHMIALGESLERTRKEKFMGWYHSHPFDLGDHSHCFLSQTDLSTQLQWQRAEDPHGNPFVAIVVDPLRS 203          
BLAST of mRNA_M-pyrifera_M_contig87100.20256.1 vs. uniprot
Match: A0A6H5JL27_9PHAE (MPN domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JL27_9PHAE)

HSP 1 Score: 151 bits (382), Expect = 5.130e-42
Identity = 74/136 (54.41%), Postives = 95/136 (69.85%), Query Frame = 1
Query:    7 HAYSGVESGLARESNPMPIEIMGLMFGHLDPDHPNELVVTDTFPLPIEGSETTVVADKPEVNAFMSELTESLSKTRKDRLCGWYHSHPFPVGEFSNCFFSAVDVQTQLSWQMNFEAMGDPFVGIVVDPLRSVAQGR 414
            HA SGVE G+A  +   P+EIMG+M G  D + PN L+VTD FPLP+EG+ET V+AD  EV  +M  L + L  TRK+R  GWYHSHPF V   S+CF S+ D+ TQLSWQ   +  G+P++ IVVDPLRS+A+ R
Sbjct:   69 HANSGVEKGMA--AGGKPVEIMGMMLGRPDTETPNALIVTDVFPLPVEGAETKVLADDQEVANYMIGLGDLLETTRKERFMGWYHSHPFDVEVHSHCFLSSTDISTQLSWQRAEDPHGNPWLAIVVDPLRSLAKSR 202          
BLAST of mRNA_M-pyrifera_M_contig87100.20256.1 vs. uniprot
Match: A0A7S1XT06_9STRA (Hypothetical protein n=2 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1XT06_9STRA)

HSP 1 Score: 151 bits (381), Expect = 5.810e-42
Identity = 76/136 (55.88%), Postives = 96/136 (70.59%), Query Frame = 1
Query:    7 HAYSGVESGLARESNPMPIEIMGLMFGHLDPDHPNELVVTDTFPLPIEGSETTVVADKPEVNAFMSELTESLSKTRKDRLCGWYHSHPFPVGEFSNCFFSAVDVQTQLSWQMNFEAMGDPFVGIVVDPLRSVAQGR 414
            HA SGVE G+  ++  +P+EIMGLM G  DP+ P  LVVTD FPLPIEG+ET V+AD        +EL E+L  TRK+R  GWYHSHPF V   S+CF SA+DV TQL WQ   +  G+P++ +VVDPLRS+A+GR
Sbjct:   56 HAISGVERGI--KTGGVPVEIMGLMIGRPDPEDPTRLVVTDVFPLPIEGAETRVLADD-------AELGENLELTRKERFMGWYHSHPFEVDIHSHCFMSAMDVSTQLGWQRLEDRNGNPWLAVVVDPLRSIAKGR 182          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig87100.20256.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8CCE0_CAFRO9.830e-5664.54MPN domain-containing protein n=3 Tax=Sar TaxID=26... [more]
A0A7S1VJP8_9STRA2.290e-4456.30Hypothetical protein n=1 Tax=Grammatophora oceanic... [more]
A0A6U3S5X1_9STRA5.140e-4455.22Hypothetical protein n=1 Tax=Ditylum brightwellii ... [more]
A0A7S4N3G5_9STRA5.740e-4455.97Hypothetical protein (Fragment) n=2 Tax=Odontella ... [more]
A0A7S2LVQ6_9STRA5.830e-4455.88Hypothetical protein (Fragment) n=1 Tax=Skeletonem... [more]
K0RC45_THAOC2.690e-4353.68MPN domain-containing protein n=1 Tax=Thalassiosir... [more]
A0A2R5GXA2_9STRA2.920e-4355.88COP9 signalosome complex subunit 5 n=1 Tax=Hondaea... [more]
B7FQK5_PHATC2.880e-4255.73COP9 SigNalosome subunit 5 n=2 Tax=Phaeodactylum t... [more]
A0A6H5JL27_9PHAE5.130e-4254.41MPN domain-containing protein n=2 Tax=Ectocarpus T... [more]
A0A7S1XT06_9STRA5.810e-4255.88Hypothetical protein n=2 Tax=Phaeomonas parva TaxI... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig87100contigM-pyrifera_M_contig87100:1..750 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score155.6
Seed ortholog evalue1.7e-35
Seed eggNOG ortholog159749.K0RC45
Preferred nameRRI1
KEGG koko:K09613
Hectar predicted targeting categoryother localisation
GOsGO:0000338,GO:0000749,GO:0000754,GO:0003674,GO:0003824,GO:0004175,GO:0004222,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0006464,GO:0006508,GO:0006807,GO:0008150,GO:0008152,GO:0008180,GO:0008233,GO:0008234,GO:0008237,GO:0009889,GO:0009891,GO:0009893,GO:0009966,GO:0009987,GO:0010033,GO:0010646,GO:0010893,GO:0016787,GO:0019216,GO:0019218,GO:0019222,GO:0019236,GO:0019538,GO:0019783,GO:0019784,GO:0023051,GO:0023058,GO:0031136,GO:0031137,GO:0031139,GO:0031323,GO:0031325,GO:0031326,GO:0031328,GO:0032443,GO:0032991,GO:0036211,GO:0042221,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0043900,GO:0043902,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044428,GO:0044446,GO:0044464,GO:0045834,GO:0045940,GO:0046889,GO:0046890,GO:0046999,GO:0048518,GO:0048522,GO:0048583,GO:0050789,GO:0050794,GO:0050810,GO:0050896,GO:0051716,GO:0062012,GO:0062013,GO:0065007,GO:0070011,GO:0070452,GO:0070646,GO:0070647,GO:0070887,GO:0071310,GO:0071444,GO:0071704,GO:0080090,GO:0106118,GO:0106120,GO:0140096,GO:1901564,GO:1902930,GO:1902932,GO:2000241,GO:2000243
EggNOG free text desc.JAB/MPN domain
EggNOG OGs2XB67@2836,COG1310@1,KOG1554@2759
COG Functional cat.OT
Best tax levelBacillariophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko01002,ko04121
Exons2
Model size414
Cds size414
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816780.7387135-CDS-M-pyrifera_M_contig87100:0..631622816780.7387135-CDS-M-pyrifera_M_contig87100:0..63Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87100 1..63 -
1692278174.5161555-CDS-M-pyrifera_M_contig87100:0..631692278174.5161555-CDS-M-pyrifera_M_contig87100:0..63Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87100 1..63 -
1622816780.7511392-CDS-M-pyrifera_M_contig87100:399..7501622816780.7511392-CDS-M-pyrifera_M_contig87100:399..750Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87100 400..750 -
1692278174.5276859-CDS-M-pyrifera_M_contig87100:399..7501692278174.5276859-CDS-M-pyrifera_M_contig87100:399..750Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig87100 400..750 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig87100.20256.1prot_M-pyrifera_M_contig87100.20256.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig87100 1..750 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig87100.20256.1

>prot_M-pyrifera_M_contig87100.20256.1 ID=prot_M-pyrifera_M_contig87100.20256.1|Name=mRNA_M-pyrifera_M_contig87100.20256.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=138bp
VRHAYSGVESGLARESNPMPIEIMGLMFGHLDPDHPNELVVTDTFPLPIE
GSETTVVADKPEVNAFMSELTESLSKTRKDRLCGWYHSHPFPVGEFSNCF
FSAVDVQTQLSWQMNFEAMGDPFVGIVVDPLRSVAQGR
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mRNA from alignment at M-pyrifera_M_contig87100:1..750-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig87100.20256.1 ID=mRNA_M-pyrifera_M_contig87100.20256.1|Name=mRNA_M-pyrifera_M_contig87100.20256.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=750bp|location=Sequence derived from alignment at M-pyrifera_M_contig87100:1..750- (Macrocystis pyrifera P11B4 male)
GTGCGGCATGCGTATTCTGGCGTGGAGAGTGGCTTGGCTCGAGAATCGAA CCCAATGCCAATAGAGATCATGGGGTTGATGTTTGGACACTTGGATCCAG ACCACCCGAACGAGTTGGTAGTGACGGACACGTTTCCGCTCCCGATCGAA GGGTCTGAGACAACGGTGGTGGCGGACAAGCCGGAGGTGAACGCCTTCAT GTCGGAGCTCACGGAATCTCTCTCCAAAACGCGCAAAGACAGGCTCTGTG GATGGTACCACTCCCACCCATTTCCTGTCGGGGAGTTCTCCAACTGCTTC TTCTCTGCAGTCGACGTCCAAACGCAGCTCTCGTGGCAAATGAACTTTGA AGTAAGTGGTATCAAGTCGGAAGTGTGGGTGGGTGTTGGGTGCTGTGCTT TTTCTTCTACTTCTTCCTTTCTTTTATATTGGTTTTGGGTATTGTTTGTT CTGTGGAATCTTGAAACTCCCTCTCTCCCACTTCCTCATCACGTCCACGA ACGCACAAGTGTGTCACGAATGTGCTGTTTTCTTATTTTCTTGCGTTATT CTCTTTTCTCTTCCTCTTTCCTCCTCCTCTTCCTCCACTTCTTTCCTCCC CCCCTTTCCTCCTCCCTCTCTCCTCCCCCCCACTTTCCACCCCCACTCCT CCCTCTCCTCCCCTCCTCCTCCTCTTTCTTCTCCTAGGCGATGGGGGACC CTTTCGTTGGGATCGTGGTGGATCCTCTCCGCTCGGTGGCACAGGGCCGT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig87100:1..750-

>mRNA_M-pyrifera_M_contig87100.20256.1 ID=mRNA_M-pyrifera_M_contig87100.20256.1|Name=mRNA_M-pyrifera_M_contig87100.20256.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=828bp|location=Sequence derived from alignment at M-pyrifera_M_contig87100:1..750- (Macrocystis pyrifera P11B4 male)
GTGCGGCATGCGTATTCTGGCGTGGAGAGTGGCTTGGCTCGAGAATCGAA
CCCAATGCCAATAGAGATCATGGGGTTGATGTTTGGACACTTGGATCCAG
ACCACCCGAACGAGTTGGTAGTGACGGACACGTTTCCGCTCCCGATCGAA
GGGTCTGAGACAACGGTGGTGGCGGACAAGCCGGAGGTGAACGCCTTCAT
GTCGGAGCTCACGGAATCTCTCTCCAAAACGCGCAAAGACAGGCTCTGTG
GATGGTACCACTCCCACCCATTTCCTGTCGGGGAGTTCTCCAACTGCTTC
TTCTCTGCAGTCGACGTCCAAACGCAGCTCTCGTGGCAAATGAACTTTGA
AGTGCGGCATGCGTATTCTGGCGTGGAGAGTGGCTTGGCTCGAGAATCGA
ACCCAATGCCAATAGAGATCATGGGGTTGATGTTTGGACACTTGGATCCA
GACCACCCGAACGAGTTGGTAGTGACGGACACGTTTCCGCTCCCGATCGA
AGGGTCTGAGACAACGGTGGTGGCGGACAAGCCGGAGGTGAACGCCTTCA
TGTCGGAGCTCACGGAATCTCTCTCCAAAACGCGCAAAGACAGGCTCTGT
GGATGGTACCACTCCCACCCATTTCCTGTCGGGGAGTTCTCCAACTGCTT
CTTCTCTGCAGTCGACGTCCAAACGCAGCTCTCGTGGCAAATGAACTTTG
AAGCGATGGGGGACCCTTTCGTTGGGATCGTGGTGGATCCTCTCCGCTCG
GTGGCACAGGGCCGTGCGATGGGGGACCCTTTCGTTGGGATCGTGGTGGA
TCCTCTCCGCTCGGTGGCACAGGGCCGT
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