mRNA_M-pyrifera_M_contig84173.19638.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig84173.19638.1
Unique NamemRNA_M-pyrifera_M_contig84173.19638.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig84173.19638.1 vs. uniprot
Match: A0A7K2AKH5_9ACTN (DUF1524 domain-containing protein n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A7K2AKH5_9ACTN)

HSP 1 Score: 111 bits (278), Expect = 1.370e-24
Identity = 59/150 (39.33%), Postives = 83/150 (55.33%), Query Frame = 1
Query:   61 WGVGLIASDNCDTRCELLAETQRVDGTWFSSYDGFVSSDPSDFQVDHLVSVNEAHRSGGAAWSLERKAAFYND-RSTGELRVVSGTSNQTKSNKGPSTDSVRYWLPSRGATDQTVTCDFVADWIHVKAVWGLSVDVDEHEALLNLSRSCG 507
            +G     S  C+TRC +L + +R DGTW S YDG V+SD S   +DH+V + EAH SG   WS  R+  + ND R    L  VS +SN++K ++ PS      W PS    +++  C +  DW+ VK  WGL+ D  E  AL  +  +CG
Sbjct:  314 YGSWTRVSSGCNTRCAVLDDERRADGTWLSWYDGVVTSDSSRLDIDHMVPLAEAHSSGAWQWSSSRRRDYANDLRHPEALAAVSASSNRSKGSRDPS-----EWKPS----NRSAWCRYAQDWVTVKVAWGLTADRAEVGALREMLDTCG 454          
BLAST of mRNA_M-pyrifera_M_contig84173.19638.1 vs. uniprot
Match: A0A2T0QAG3_9ACTN (Uncharacterized protein DUF1524 n=1 Tax=Allonocardiopsis opalescens TaxID=1144618 RepID=A0A2T0QAG3_9ACTN)

HSP 1 Score: 105 bits (262), Expect = 2.700e-24
Identity = 58/151 (38.41%), Postives = 82/151 (54.30%), Query Frame = 1
Query:   82 SDNCDTRCELLA----------ETQRVDGTWFSSYDGFVSSDPSDFQVDHLVSVNEAHRSGGAAWSLERKAAFYNDRSTGELRVVSGTSNQTKSNKGPSTDSVRYWLPSRGATDQTVTCDFVADWIHVKAVWGLSVDVDEHEALLNLSRSC 504
            S NC+ R  +L           +     G+W+S YDG   S PS  Q+DH++ + EA RSG   WS  R+  F ND +T +L  VSG+SNQ+KS++ P+      W+P+R A    V C +   W++VK VW LSVD  E  AL ++   C
Sbjct:   68 SGNCNARETVLRRDGTNVTVGNDCYPTSGSWYSPYDGVTLSSPSQVQIDHMIPLAEAWRSGADTWSTARREDFANDLATAQLWAVSGSSNQSKSDQDPA-----EWMPTRSA----VHCLYARAWVNVKYVWRLSVDSAERSALTSILNRC 209          
BLAST of mRNA_M-pyrifera_M_contig84173.19638.1 vs. uniprot
Match: A0A1Q9TXJ8_9ACTN (DUF1524 domain-containing protein n=1 Tax=Nocardiopsis sp. CNR-923 TaxID=1904965 RepID=A0A1Q9TXJ8_9ACTN)

HSP 1 Score: 106 bits (264), Expect = 3.010e-24
Identity = 59/158 (37.34%), Postives = 82/158 (51.90%), Query Frame = 1
Query:   61 WGVGLIASDNCDTRCELLA----------ETQRVDGTWFSSYDGFVSSDPSDFQVDHLVSVNEAHRSGGAAWSLERKAAFYNDRSTGELRVVSGTSNQTKSNKGPSTDSVRYWLPSRGATDQTVTCDFVADWIHVKAVWGLSVDVDEHEALLNLSRSC 504
            W  G+   D+C TR  +L           + Q V GTW+S YDG   ++  D  +DH+V +NEA RSG A W+ E +  F ND    +L  VS +SN++K +  PS      WLP      ++  C++ A WI VK  W L+VD DE EAL  +   C
Sbjct:   97 WDTGV--QDDCTTRQAVLLRAGEAVEVDEDCQPVSGTWYSVYDGETLTEAGDLDIDHMVPLNEAWRSGAATWATEERRRFANDLDGPQLWAVSASSNRSKGDADPSD-----WLPPL----ESFHCEYTAHWIEVKHTWNLAVDPDEEEALRTVLAGC 243          
BLAST of mRNA_M-pyrifera_M_contig84173.19638.1 vs. uniprot
Match: A0A017HH59_9RHOB (Putative secreted protein n=2 Tax=Rubellimicrobium TaxID=295418 RepID=A0A017HH59_9RHOB)

HSP 1 Score: 104 bits (260), Expect = 3.250e-24
Identity = 67/185 (36.22%), Postives = 96/185 (51.89%), Query Frame = 1
Query:    1 LDVQSERKANYERGDWEQGGWGVGLIASDNC-DTRCELLA---------ETQRVD---GTWFSSYDGFVSSDPSDFQVDHLVSVNEAHRSGGAAWSLERKAAFYNDRSTGE-LRVVSGTSNQTKSNKGPSTDSVRYWLPSRGATDQTVTCDFVADWIHVKAVWGLSVDVDEHEALLNLSRSCGWE 513
            + V++ER   ++R DW    W   L    +C D R E+LA         +TQR D   G W  +Y G    DP    VDH+V + EA RSGG  W +ER+AA+ ND   G  L  VS ++N++K ++GP       WLP     + +  C +VADW+ VK  W LS+D  E   + N+ R+C  +
Sbjct:   19 IPVEAERPLGFDREDWPH--W---LDTDRDCQDARQEVLAAESEVPVRWDTQRCDVVSGLWRDAYTGQTVRDPRALDVDHMVPLAEAFRSGGRDWDMERRAAYANDLQDGRTLIAVSASANRSKGDQGP-----EDWLPP----EVSYRCRYVADWVAVKVRWSLSMDERERVTVGNILRACAQD 189          
BLAST of mRNA_M-pyrifera_M_contig84173.19638.1 vs. uniprot
Match: A0A368T0F9_9ACTN (HNH endonuclease n=1 Tax=Marinitenerispora sediminis TaxID=1931232 RepID=A0A368T0F9_9ACTN)

HSP 1 Score: 104 bits (259), Expect = 8.410e-24
Identity = 59/150 (39.33%), Postives = 79/150 (52.67%), Query Frame = 1
Query:   73 LIASDNCDTRCELLA----------ETQRVDGTWFSSYDGFVSSDPSDFQVDHLVSVNEAHRSGGAAWSLERKAAFYNDRSTGELRVVSGTSNQTKSNKGPSTDSVRYWLPSRGATDQTVTCDFVADWIHVKAVWGLSVDVDEHEALLNL 492
            +    NC+ R  +L           +     GTW S YDG  +S PS  Q+DH+V + EA RSG   W+  R+ +F ND  T +L  VSG+SNQ+K +K PS      WLP       ++ C +V  WI+VK VW LSVD  E  AL N+
Sbjct:   69 IAVEGNCNAREYVLRRDGANVTVGNDCYPTSGTWTSPYDGVRTSTPSQVQIDHMVPLAEAWRSGADTWTTSRRQSFANDLDTPQLWAVSGSSNQSKGDKDPS-----EWLPP----STSIHCTYVRSWINVKYVWNLSVDSAERSALQNV 209          
BLAST of mRNA_M-pyrifera_M_contig84173.19638.1 vs. uniprot
Match: A0A6J6UUI1_9ZZZZ (Unannotated protein n=4 Tax=root TaxID=1 RepID=A0A6J6UUI1_9ZZZZ)

HSP 1 Score: 107 bits (267), Expect = 1.270e-23
Identity = 61/148 (41.22%), Postives = 87/148 (58.78%), Query Frame = 1
Query:   85 DNCDTRCELLAETQRV-------DGTWFSSYDGFVSSDPSDFQVDHLVSVNEAHRSGGAAWSLERKAAFYNDRSTGE-LRVVSGTSNQTKSNKGPSTDSVRYWLPSRGATDQTVTCDFVADWIHVKAVWGLSVDVDEHEALLNLSRSC 504
            D CDTRCE+L + +R+       +G W S YDG V+ +PS+F +DHLV++ EA RSG + W   R+ AF ND      L  VS +SN++KS++ PS+     W P     D+   C +V DW+ VK  WGL+VD  E  AL ++  +C
Sbjct:  248 DGCDTRCEVL-KVERLPSVAGLSNGGWLSLYDGKVTDNPSEFDIDHLVALGEAWRSGASEWDGARRQAFANDLDDPRALIAVSASSNRSKSDRDPSS-----WQPP----DKAYWCTYVTDWMAVKLKWGLAVDPIEFTALQSIVEAC 385          
BLAST of mRNA_M-pyrifera_M_contig84173.19638.1 vs. uniprot
Match: A0A6J6XVQ6_9ZZZZ (Unannotated protein n=1 Tax=freshwater metagenome TaxID=449393 RepID=A0A6J6XVQ6_9ZZZZ)

HSP 1 Score: 101 bits (252), Expect = 2.400e-23
Identity = 58/148 (39.19%), Postives = 85/148 (57.43%), Query Frame = 1
Query:   85 DNCDTRCELLAETQRV-------DGTWFSSYDGFVSSDPSDFQVDHLVSVNEAHRSGGAAWSLERKAAFYNDRSTGE-LRVVSGTSNQTKSNKGPSTDSVRYWLPSRGATDQTVTCDFVADWIHVKAVWGLSVDVDEHEALLNLSRSC 504
            + CDTRCE+L + +R+        G W S YDG V+ +PS+F +DHLV++ EA RSG + W   R+ AF ND      L  V+ +SN++KS++ PS+     W P     D+   C +V +W+ VK  WGL+VD  E  AL ++   C
Sbjct:   24 NGCDTRCEVL-KIERLPSLPGLPSGGWLSLYDGKVTENPSEFDIDHLVALGEAWRSGASEWDGARRQAFANDLDEPRALIAVTASSNRSKSDRDPSS-----WQPP----DKAYWCTYVTEWMAVKLKWGLAVDPIEFTALQSIVEGC 161          
BLAST of mRNA_M-pyrifera_M_contig84173.19638.1 vs. uniprot
Match: UPI0004756B35 (HNH endonuclease family protein n=2 Tax=Nocardiopsis TaxID=2013 RepID=UPI0004756B35)

HSP 1 Score: 103 bits (256), Expect = 4.170e-23
Identity = 58/158 (36.71%), Postives = 80/158 (50.63%), Query Frame = 1
Query:   61 WGVGLIASDNCDTRCELLAET----------QRVDGTWFSSYDGFVSSDPSDFQVDHLVSVNEAHRSGGAAWSLERKAAFYNDRSTGELRVVSGTSNQTKSNKGPSTDSVRYWLPSRGATDQTVTCDFVADWIHVKAVWGLSVDVDEHEALLNLSRSC 504
            W  G+   DNC TR  +L             Q V G+W S YD  V +D  D  +DH+V++ EA RSG  AW+ E + +F ND  T +L  VS +SN+ K +  P+      W+P      + V CD+ A WI VK  W L+VD DE + L  +   C
Sbjct:   94 WDSGV--EDNCTTRQVVLERDGEDVESDQNCQPVSGSWSSPYDDEVLTDVQDVDIDHMVALKEAWRSGAHAWTTEERQSFANDLDTTQLWAVSASSNRAKGDADPAD-----WMPP----SEAVHCDYAASWIDVKHTWDLTVDPDEEQTLREILEGC 240          
BLAST of mRNA_M-pyrifera_M_contig84173.19638.1 vs. uniprot
Match: A0A4S2REQ3_9ACTN (HNH endonuclease n=1 Tax=Streptomyces sp. A1547 TaxID=2563105 RepID=A0A4S2REQ3_9ACTN)

HSP 1 Score: 102 bits (255), Expect = 4.490e-23
Identity = 64/180 (35.56%), Postives = 93/180 (51.67%), Query Frame = 1
Query:    1 LDVQSERKANYERGDWEQGGWGVGLIASDNCDTRCE-LLAET----------QRVDGTWFSSYDGFVSSDPSDFQVDHLVSVNEAHRSGGAAWSLERKAAFYNDRSTGE-LRVVSGTSNQTKSNKGPSTDSVRYWLPSRGATDQTVTCDFVADWIHVKAVWGLSVDVDEHEALLNLSRSC 504
            L V  E +  Y+R  ++   W  GLIASD CDTR E +L+E           +   G W S+YD  V +D +   VDH V + E + S    W   R+ A+ ND+++ + L  VS  SN++K++K P+      WLP    TD +  C + A W+  K  W L+VD  E +ALL L+  C
Sbjct:   49 LSVAEEHREGYKRDLYKH--WNKGLIASDGCDTRKEVILSEALEAPTVASGCKLTGGLWRSAYDDVVVTDAARLDVDHFVPLAEVYDSEQTPWDAARREAYANDQNSPDTLIAVSAASNRSKADKDPA-----EWLP----TDGSYHCTYAATWVGTKLRWDLAVDEAERQALLGLAEDC 217          
BLAST of mRNA_M-pyrifera_M_contig84173.19638.1 vs. uniprot
Match: A0A6I3GEZ3_9ACTN (DUF1524 domain-containing protein n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A6I3GEZ3_9ACTN)

HSP 1 Score: 101 bits (252), Expect = 7.440e-23
Identity = 58/148 (39.19%), Postives = 85/148 (57.43%), Query Frame = 1
Query:   85 DNCDTRCELLAETQRV-------DGTWFSSYDGFVSSDPSDFQVDHLVSVNEAHRSGGAAWSLERKAAFYNDRSTGE-LRVVSGTSNQTKSNKGPSTDSVRYWLPSRGATDQTVTCDFVADWIHVKAVWGLSVDVDEHEALLNLSRSC 504
            + CDTRCE+L + +R+        G W S YDG V+ +PS+F +DHLV++ EA RSG + W   R+ AF ND      L  V+ +SN++KS++ PS+     W P     D+   C +V +W+ VK  WGL+VD  E  AL ++   C
Sbjct:   69 NGCDTRCEVL-KIERLPSLPGLPSGGWLSLYDGKVTENPSEFDIDHLVALGEAWRSGASEWDGARRQAFANDLDEPRALIAVTASSNRSKSDRDPSS-----WQPP----DKAYWCTYVTEWMAVKLKWGLAVDPIEFTALQSIVEGC 206          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig84173.19638.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7K2AKH5_9ACTN1.370e-2439.33DUF1524 domain-containing protein n=1 Tax=Acidimic... [more]
A0A2T0QAG3_9ACTN2.700e-2438.41Uncharacterized protein DUF1524 n=1 Tax=Allonocard... [more]
A0A1Q9TXJ8_9ACTN3.010e-2437.34DUF1524 domain-containing protein n=1 Tax=Nocardio... [more]
A0A017HH59_9RHOB3.250e-2436.22Putative secreted protein n=2 Tax=Rubellimicrobium... [more]
A0A368T0F9_9ACTN8.410e-2439.33HNH endonuclease n=1 Tax=Marinitenerispora sedimin... [more]
A0A6J6UUI1_9ZZZZ1.270e-2341.22Unannotated protein n=4 Tax=root TaxID=1 RepID=A0A... [more]
A0A6J6XVQ6_9ZZZZ2.400e-2339.19Unannotated protein n=1 Tax=freshwater metagenome ... [more]
UPI0004756B354.170e-2336.71HNH endonuclease family protein n=2 Tax=Nocardiops... [more]
A0A4S2REQ3_9ACTN4.490e-2335.56HNH endonuclease n=1 Tax=Streptomyces sp. A1547 Ta... [more]
A0A6I3GEZ3_9ACTN7.440e-2339.19DUF1524 domain-containing protein n=1 Tax=Actinomy... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig84173contigM-pyrifera_M_contig84173:94..816 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score109.0
Seed ortholog evalue3.1e-21
Seed eggNOG ortholog1304865.JAGF01000001_gene278
KEGG koko:K01406,ko:K07004
KEGG Pathwayko01503,map01503
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Protein of unknown function (DUF1524)
EggNOG OGs2GJ38@201174,COG2340@1,COG2340@2,COG2356@1,COG2356@2
EC3.4.24.40
COG Functional cat.L
Best tax levelActinobacteria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01002
Exons1
Model size723
Cds size723
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816749.221864-CDS-M-pyrifera_M_contig84173:93..8161622816749.221864-CDS-M-pyrifera_M_contig84173:93..816Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84173 94..816 -
1692278155.4196231-CDS-M-pyrifera_M_contig84173:93..8161692278155.4196231-CDS-M-pyrifera_M_contig84173:93..816Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84173 94..816 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig84173.19638.1prot_M-pyrifera_M_contig84173.19638.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig84173 94..816 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig84173.19638.1

>prot_M-pyrifera_M_contig84173.19638.1 ID=prot_M-pyrifera_M_contig84173.19638.1|Name=mRNA_M-pyrifera_M_contig84173.19638.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=241bp
LDVQSERKANYERGDWEQGGWGVGLIASDNCDTRCELLAETQRVDGTWFS
SYDGFVSSDPSDFQVDHLVSVNEAHRSGGAAWSLERKAAFYNDRSTGELR
VVSGTSNQTKSNKGPSTDSVRYWLPSRGATDQTVTCDFVADWIHVKAVWG
LSVDVDEHEALLNLSRSCGWERGAASCRISPPSDAYRPKLGEWSSASVWR
LYQAFFLRQPDAAGFNYWLDQNRQGLSLEAMASEFAVSTEF
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mRNA from alignment at M-pyrifera_M_contig84173:94..816-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig84173.19638.1 ID=mRNA_M-pyrifera_M_contig84173.19638.1|Name=mRNA_M-pyrifera_M_contig84173.19638.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=723bp|location=Sequence derived from alignment at M-pyrifera_M_contig84173:94..816- (Macrocystis pyrifera P11B4 male)
TTGGACGTGCAAAGCGAACGCAAAGCGAACTACGAACGCGGCGACTGGGA GCAGGGCGGCTGGGGTGTGGGTCTGATCGCCAGCGACAACTGCGACACAC GCTGCGAACTCCTCGCCGAAACCCAGCGAGTCGATGGGACCTGGTTCTCG AGCTATGACGGGTTCGTGTCGTCGGACCCGTCCGATTTCCAGGTCGATCA TCTGGTGTCGGTGAATGAGGCGCATCGTTCCGGCGGCGCTGCCTGGTCTC TGGAGCGCAAGGCGGCGTTCTACAACGACCGCAGCACCGGTGAGCTTCGA GTCGTCTCGGGGACGAGTAATCAGACCAAGTCGAACAAGGGCCCGTCGAC TGATTCGGTCCGCTATTGGTTGCCGAGCCGCGGCGCCACCGACCAAACCG TGACATGTGACTTCGTCGCCGACTGGATTCATGTGAAGGCGGTGTGGGGG CTGTCGGTCGATGTCGACGAACATGAGGCCCTCCTCAATCTGTCGAGGAG TTGCGGTTGGGAACGCGGTGCCGCGTCCTGCCGGATCTCTCCGCCGTCCG ACGCGTATCGACCGAAGTTGGGGGAGTGGTCCTCGGCGTCGGTATGGCGG CTGTATCAAGCGTTCTTCCTCCGCCAACCCGATGCGGCCGGCTTCAACTA CTGGCTCGACCAGAACCGGCAAGGCCTGAGTCTGGAGGCGATGGCCAGCG AGTTCGCTGTATCCACAGAGTTC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig84173:94..816-

>mRNA_M-pyrifera_M_contig84173.19638.1 ID=mRNA_M-pyrifera_M_contig84173.19638.1|Name=mRNA_M-pyrifera_M_contig84173.19638.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1446bp|location=Sequence derived from alignment at M-pyrifera_M_contig84173:94..816- (Macrocystis pyrifera P11B4 male)
TTGGACGTGCAAAGCGAACGCAAAGCGAACTACGAACGCGGCGACTGGGA
GCAGGGCGGCTGGGGTGTGGGTCTGATCGCCAGCGACAACTGCGACACAC
GCTGCGAACTCCTCGCCGAAACCCAGCGAGTCGATGGGACCTGGTTCTCG
AGCTATGACGGGTTCGTGTCGTCGGACCCGTCCGATTTCCAGGTCGATCA
TCTGGTGTCGGTGAATGAGGCGCATCGTTCCGGCGGCGCTGCCTGGTCTC
TGGAGCGCAAGGCGGCGTTCTACAACGACCGCAGCACCGGTGAGCTTCGA
GTCGTCTCGGGGACGAGTAATCAGACCAAGTCGAACAAGGGCCCGTCGAC
TGATTCGGTCCGCTATTGGTTGCCGAGCCGCGGCGCCACCGACCAAACCG
TGACATGTGACTTCGTCGCCGACTGGATTCATGTGAAGGCGGTGTGGGGG
CTGTCGGTCGATGTCGACGAACATGAGGCCCTCCTCAATCTGTCGAGGAG
TTGCGGTTGGGAACGCGGTGCCGCGTCCTGCCGGATCTCTCCGCCGTCCG
ACGCGTATCGACCGAAGTTGGGGGAGTGGTCCTCGGCGTCGGTATGGCGG
CTGTATCAAGCGTTCTTCCTCCGCCAACCCGATGCGGCCGGCTTCAACTA
CTGGCTCGACCAGAACCGGCAAGGCCTGAGTCTGGAGGCGATGGCCAGCG
AGTTCGCTGTATCCACAGAGTTCTTGGACGTGCAAAGCGAACGCAAAGCG
AACTACGAACGCGGCGACTGGGAGCAGGGCGGCTGGGGTGTGGGTCTGAT
CGCCAGCGACAACTGCGACACACGCTGCGAACTCCTCGCCGAAACCCAGC
GAGTCGATGGGACCTGGTTCTCGAGCTATGACGGGTTCGTGTCGTCGGAC
CCGTCCGATTTCCAGGTCGATCATCTGGTGTCGGTGAATGAGGCGCATCG
TTCCGGCGGCGCTGCCTGGTCTCTGGAGCGCAAGGCGGCGTTCTACAACG
ACCGCAGCACCGGTGAGCTTCGAGTCGTCTCGGGGACGAGTAATCAGACC
AAGTCGAACAAGGGCCCGTCGACTGATTCGGTCCGCTATTGGTTGCCGAG
CCGCGGCGCCACCGACCAAACCGTGACATGTGACTTCGTCGCCGACTGGA
TTCATGTGAAGGCGGTGTGGGGGCTGTCGGTCGATGTCGACGAACATGAG
GCCCTCCTCAATCTGTCGAGGAGTTGCGGTTGGGAACGCGGTGCCGCGTC
CTGCCGGATCTCTCCGCCGTCCGACGCGTATCGACCGAAGTTGGGGGAGT
GGTCCTCGGCGTCGGTATGGCGGCTGTATCAAGCGTTCTTCCTCCGCCAA
CCCGATGCGGCCGGCTTCAACTACTGGCTCGACCAGAACCGGCAAGGCCT
GAGTCTGGAGGCGATGGCCAGCGAGTTCGCTGTATCCACAGAGTTC
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