mRNA_M-pyrifera_M_contig82646.19320.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig82646.19320.1
Unique NamemRNA_M-pyrifera_M_contig82646.19320.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig82646.19320.1 vs. uniprot
Match: UPI000D73EC24 (LOW QUALITY PROTEIN: iduronate 2-sulfatase-like n=1 Tax=Pomacea canaliculata TaxID=400727 RepID=UPI000D73EC24)

HSP 1 Score: 168 bits (425), Expect = 2.610e-46
Identity = 89/175 (50.86%), Postives = 113/175 (64.57%), Query Frame = 1
Query:    1 LFLVVDDMRNEIGCYGVEAMHTPNMDAFAADPDTVTFERAYVQQAICNPSRTSFLTSRRPDTTRVYDLVHYWRNVGGNFTTIPEHFKLSGYTTAGAGKVFHPLGQSGHLDDEPYSWSPQYPYFHASAESNQ-----------KYANFSHCFYNITDVSDDQLPDGEITQHTLSTL 492
            LFLVVDD+R ++GCYG   M TPN+D  A+   +V F RAY+QQA+C+PSRTSFLT RRPDTTR++DL  Y+RN+ GNFTT+P+HFK SGY T   GK+FHP   SG  DD PYSWS   P  HA +ES +           KY + + C  N+ +  D  LPD + T   +  L
Sbjct:   30 LFLVVDDLRPQLGCYGQTFMSTPNVDQLASR--SVRFNRAYIQQAVCSPSRTSFLTGRRPDTTRIFDLSTYFRNISGNFTTLPQHFKSSGYVTQSVGKIFHPGVASGGTDDYPYSWSN--PAHHAPSESQRTAKVCPGPGGNKYFS-ARCPVNVQEQPDKTLPDIQNTDFAIQFL 199          
BLAST of mRNA_M-pyrifera_M_contig82646.19320.1 vs. uniprot
Match: A0A8B6G6P3_MYTGA (Iduronate 2-sulfatase n=2 Tax=Mytilus galloprovincialis TaxID=29158 RepID=A0A8B6G6P3_MYTGA)

HSP 1 Score: 157 bits (398), Expect = 2.990e-45
Identity = 83/175 (47.43%), Postives = 104/175 (59.43%), Query Frame = 1
Query:    1 LFLVVDDMRNEIGCYGVEAMHTPNMDAFAADPDTVTFERAYVQQAICNPSRTSFLTSRRPDTTRVYDLVHYWRNVGGNFTTIPEHFKLSGYTTAGAGKVFHPLGQSGHLDDEPYSWSPQYPYFHASAES-----------NQKYANFSHCFYNITDVSDDQLPDGEITQHTLSTL 492
            L +V DD+R  +GCYG E + TPN+D  A+   +V F R Y QQAIC PSR SFLTSRRP+TT VYD   YWR  GGNFTT+P+HFK +GY T   GK+FHP   SG  DD PYSWS   P +H S +             + Y N   C  ++  + +  LPD + TQH L  +
Sbjct:   25 LLIVADDLRPSLGCYGYEPLITPNIDLLASQ--SVVFNRTYAQQAICGPSRASFLTSRRPNTTHVYDQHTYWRKRGGNFTTLPQHFKNNGYVTFSVGKIFHPGAASGKTDDYPYSWSV--PPYHPSTQQYKMAKVCPGPDGKLYMNIV-CPVDVIAMPEKTLPDLQSTQHALQIM 194          
BLAST of mRNA_M-pyrifera_M_contig82646.19320.1 vs. uniprot
Match: UPI001B88C141 (iduronate 2-sulfatase-like n=1 Tax=Gigantopelta aegis TaxID=1735272 RepID=UPI001B88C141)

HSP 1 Score: 164 bits (416), Expect = 8.600e-45
Identity = 88/178 (49.44%), Postives = 111/178 (62.36%), Query Frame = 1
Query:    1 LFLVVDDMRNEIGCYGVEAMHTPNMDAFAADPDTVTFERAYVQQAICNPSRTSFLTSRRPDTTRVYDLVHYWRNVGGNFTTIPEHFKLSGYTTAGAGKVFHPLGQSGHLDDEPYSWSPQYPYFHASAES-----------NQKYANFSHCFYNITDVSDDQLPDGEITQ---HTLSTL 492
            L +VVDD+R  +GCYG E + TPN+D  A+   +V F +AYVQQA+C PSRTSFLTSRRPDTTR+YD   YWRN  GNFTT+P+HFK +GY     GKVFHP   S + DD PYSWS  +P +H   E             +KY N   C  ++++     LPD +ITQ   H L T+
Sbjct:   27 LIIVVDDLRPTLGCYGSEHLITPNIDNLASR--SVVFNKAYVQQALCGPSRTSFLTSRRPDTTRLYDFYSYWRNAAGNFTTLPQHFKENGYVAQSVGKVFHPGIASNYTDDYPYSWS--FPAYHPPTEQYKMAKVCPDPDGKKYMNLV-CPVSVSEQPGGSLPDIQITQFATHFLKTM 199          
BLAST of mRNA_M-pyrifera_M_contig82646.19320.1 vs. uniprot
Match: A0A8B6DN57_MYTGA (Iduronate 2-sulfatase n=2 Tax=Mytilus galloprovincialis TaxID=29158 RepID=A0A8B6DN57_MYTGA)

HSP 1 Score: 157 bits (398), Expect = 2.080e-44
Identity = 83/175 (47.43%), Postives = 104/175 (59.43%), Query Frame = 1
Query:    1 LFLVVDDMRNEIGCYGVEAMHTPNMDAFAADPDTVTFERAYVQQAICNPSRTSFLTSRRPDTTRVYDLVHYWRNVGGNFTTIPEHFKLSGYTTAGAGKVFHPLGQSGHLDDEPYSWSPQYPYFHASAES-----------NQKYANFSHCFYNITDVSDDQLPDGEITQHTLSTL 492
            L +V DD+R  +GCYG E + TPN+D  A+   +V F R Y QQAIC PSR SFLTSRRP+TT VYD   YWR  GGNFTT+P+HFK +GY T   GK+FHP   SG  DD PYSWS   P +H S +             + Y N   C  ++  + +  LPD + TQH L  +
Sbjct:   25 LLIVADDLRPSLGCYGYEPLITPNIDLLASQ--SVVFNRTYAQQAICGPSRASFLTSRRPNTTHVYDQHTYWRKRGGNFTTLPQHFKNNGYVTFSVGKIFHPGAASGKTDDYPYSWSV--PPYHPSTQQYKMAKVCPGPDGKLYMNIV-CPVDVIAMPEKTLPDLQSTQHALQIM 194          
BLAST of mRNA_M-pyrifera_M_contig82646.19320.1 vs. uniprot
Match: UPI000719A88A (iduronate 2-sulfatase-like isoform X3 n=1 Tax=Priapulus caudatus TaxID=37621 RepID=UPI000719A88A)

HSP 1 Score: 156 bits (395), Expect = 5.440e-44
Identity = 84/173 (48.55%), Postives = 105/173 (60.69%), Query Frame = 1
Query:    1 LFLVVDDMRNEIGCYGVEAMHTPNMDAFAADPDTVTFERAYVQQAICNPSRTSFLTSRRPDTTRVYDLVHYWRNVGGNFTTIPEHFKLSGYTTAGAGKVFHPLGQSGHLDDEPYSWS--PQYPYFHASAESN-------QKYANFSHCFYNITDVSDDQLPDGEITQHTLSTL 492
            LF+VVDD+R  +GCY V    TPN+D   +    + F  AY QQA+C PSR SFLTSRRPDTTR+YD+  YWR+  GNFTT+P+HFK  GY TA  GKVFHP   S   DD+PYSWS  P +P   A   S        +KY+N   C  N+T   +  LPD + T+  +  L
Sbjct:   41 LFIVVDDLRPALGCYEVSKYITPNIDQLCSMG--IKFSNAYAQQALCGPSRVSFLTSRRPDTTRLYDVHSYWRHAAGNFTTLPQHFKEHGYHTASVGKVFHPGKSSNFNDDQPYSWSEKPYHPSTQAYKNSKVCAGPGGRKYSNIV-CPVNVTQQPEATLPDMQSTRQAMQFL 210          
BLAST of mRNA_M-pyrifera_M_contig82646.19320.1 vs. uniprot
Match: A0A8J5CGT6_CHIOP (Iduronate 2-sulfatase n=1 Tax=Chionoecetes opilio TaxID=41210 RepID=A0A8J5CGT6_CHIOP)

HSP 1 Score: 156 bits (395), Expect = 1.220e-43
Identity = 83/172 (48.26%), Postives = 106/172 (61.63%), Query Frame = 1
Query:    1 LFLVVDDMRNEIGCYGVEAMHTPNMDAFAADPDTVTFERAYVQQAICNPSRTSFLTSRRPDTTRVYDLVHYWRNVGGNFTTIPEHFKLSGYTTAGAGKVFHPLGQSGHLDDEPYSWS--PQYPYFHASAE------SNQKYANFSHCFYNITDVSDDQLPDGEITQHTLSTL 492
            L LVVDD+R  +GCYG     TPN+DA A D     F RAY QQA+C PSRTSFLTSRRPDTTR+YD+  YWR   GNFTT+P++F+  GY T  AGK+FHP   S H DD+PYSWS  P +P   A  +      S+       +C   + +     LPD + T+H+++ L
Sbjct:   25 LLLVVDDLRPVLGCYGDPVAVTPNIDALARD--ATVFTRAYAQQALCGPSRTSFLTSRRPDTTRLYDVHSYWRRHAGNFTTLPQYFRDHGYHTVSAGKIFHPGIVSNHSDDQPYSWSEAPYHPPTQAHKQDPVCLGSDGSLHTNIYCPVQVEEQPGGSLPDIQTTRHSIAWL 194          
BLAST of mRNA_M-pyrifera_M_contig82646.19320.1 vs. uniprot
Match: A0A6J8ERT3_MYTCO (IDS n=1 Tax=Mytilus coruscus TaxID=42192 RepID=A0A6J8ERT3_MYTCO)

HSP 1 Score: 154 bits (389), Expect = 2.420e-43
Identity = 73/128 (57.03%), Postives = 88/128 (68.75%), Query Frame = 1
Query:    1 LFLVVDDMRNEIGCYGVEAMHTPNMDAFAADPDTVTFERAYVQQAICNPSRTSFLTSRRPDTTRVYDLVHYWRNVGGNFTTIPEHFKLSGYTTAGAGKVFHPLGQSGHLDDEPYSWSPQYPYFHASAE 384
            L +V DD+R  +GCYG E + TPN+D  A+   +V F R Y QQAIC PSR SFLTSRRP+TT VYD   YWR  GGNFTT+P+HFK +GY T   GK+FHP   SG  DD PYSWS  +P +H S +
Sbjct:   24 LLIVADDLRPSLGCYGYEPLITPNIDLLASQ--SVVFNRTYAQQAICGPSRASFLTSRRPNTTHVYDQHTYWRKRGGNFTTLPQHFKNNGYVTFSVGKIFHPGAASGKTDDYPYSWS--FPPYHPSTQ 147          
BLAST of mRNA_M-pyrifera_M_contig82646.19320.1 vs. uniprot
Match: UPI001E1D7C3A (iduronate 2-sulfatase-like isoform X1 n=3 Tax=Mercenaria mercenaria TaxID=6596 RepID=UPI001E1D7C3A)

HSP 1 Score: 159 bits (403), Expect = 6.040e-43
Identity = 78/128 (60.94%), Postives = 90/128 (70.31%), Query Frame = 1
Query:    1 LFLVVDDMRNEIGCYGVEAMHTPNMDAFAADPDTVTFERAYVQQAICNPSRTSFLTSRRPDTTRVYDLVHYWRNVGGNFTTIPEHFKLSGYTTAGAGKVFHPLGQSGHLDDEPYSWSPQYPYFHASAE 384
            LF+VVDD+R  +GCYG + M TPN+D  A    +V FE AYVQQA+C PSR SFLTSRRPDTTR+YD   YWR   GNFTT+P+HFK +GY T   GKVFHP   S H DD PYSWS   P +H S E
Sbjct:   48 LFIVVDDLRPSLGCYGDDYMVTPNIDNLAKK--SVLFENAYVQQALCGPSRVSFLTSRRPDTTRLYDFYSYWRVAAGNFTTLPQHFKENGYHTMSIGKVFHPGKSSNHSDDYPYSWSV--PAYHPSTE 171          
BLAST of mRNA_M-pyrifera_M_contig82646.19320.1 vs. uniprot
Match: A0A834BU08_ORYME (Iduronate 2-sulfatase n=1 Tax=Oryzias melastigma TaxID=30732 RepID=A0A834BU08_ORYME)

HSP 1 Score: 150 bits (379), Expect = 8.630e-43
Identity = 81/175 (46.29%), Postives = 105/175 (60.00%), Query Frame = 1
Query:    1 LFLVVDDMRNEIGCYGVEAMHTPNMDAFAADPDTVTFERAYVQQAICNPSRTSFLTSRRPDTTRVYDLVHYWRNVGGNFTTIPEHFKLSGYTTAGAGKVFHPLGQSGHLDDEPYSWSPQYPYFHASA-----------ESNQKYANFSHCFYNITDVSDDQLPDGEITQHTLSTL 492
            LF+V DD+R  +GCYG   + +PN+D  A+   ++ F  A+ QQA+C PSRTS LTSRRPDTTR+YD   YWR   GNF+T+P++FK +GY TA  GKVFHP   S H DD PYSWS   P +H ++           E  + +AN   C  N+T+     LPD E T   L  L
Sbjct:   29 LFIVADDLRTSLGCYGDPLIKSPNIDQLASK--SLVFLNAFSQQAVCAPSRTSMLTSRRPDTTRLYDFNSYWRTHSGNFSTLPQYFKSNGYFTASVGKVFHPGIASNHSDDYPYSWS--IPAYHPASFRFEKAKMCKGEDGKLHANLL-CAVNVTEQPGGTLPDLESTDEALRLL 198          
BLAST of mRNA_M-pyrifera_M_contig82646.19320.1 vs. uniprot
Match: A0A8C9KC90_PANTA (Iduronate 2-sulfatase n=1 Tax=Panthera tigris altaica TaxID=74533 RepID=A0A8C9KC90_PANTA)

HSP 1 Score: 153 bits (386), Expect = 1.890e-42
Identity = 79/175 (45.14%), Postives = 109/175 (62.29%), Query Frame = 1
Query:    1 LFLVVDDMRNEIGCYGVEAMHTPNMDAFAADPDTVTFERAYVQQAICNPSRTSFLTSRRPDTTRVYDLVHYWRNVGGNFTTIPEHFKLSGYTTAGAGKVFHPLGQSGHLDDEPYSWSPQYPYFHASAES-----------NQKYANFSHCFYNITDVSDDQLPDGEITQHTLSTL 492
            L ++VDD+R  +GCYG +A+ +PN+D  A+   ++ F+ A+ QQA+C PSR SFLT RRPDTTR+YD   YWR   GNF+TIP++FK +GY T   GKVFHP   S + DD PYSWS  +P +H S+E             Q +AN   C  ++ DV +  LPD + T+  +  L
Sbjct:   35 LLIIVDDLRPSLGCYGDKAIRSPNIDQLASR--SLLFQNAFAQQAVCAPSRVSFLTGRRPDTTRLYDFNSYWRVHAGNFSTIPQYFKENGYVTMSVGKVFHPGISSNYSDDSPYSWS--FPPYHPSSEKYENTKTCRGPDGQLHANLL-CPVDVADVPEGTLPDKQSTEQAIRLL 204          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig82646.19320.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI000D73EC242.610e-4650.86LOW QUALITY PROTEIN: iduronate 2-sulfatase-like n=... [more]
A0A8B6G6P3_MYTGA2.990e-4547.43Iduronate 2-sulfatase n=2 Tax=Mytilus galloprovinc... [more]
UPI001B88C1418.600e-4549.44iduronate 2-sulfatase-like n=1 Tax=Gigantopelta ae... [more]
A0A8B6DN57_MYTGA2.080e-4447.43Iduronate 2-sulfatase n=2 Tax=Mytilus galloprovinc... [more]
UPI000719A88A5.440e-4448.55iduronate 2-sulfatase-like isoform X3 n=1 Tax=Pria... [more]
A0A8J5CGT6_CHIOP1.220e-4348.26Iduronate 2-sulfatase n=1 Tax=Chionoecetes opilio ... [more]
A0A6J8ERT3_MYTCO2.420e-4357.03IDS n=1 Tax=Mytilus coruscus TaxID=42192 RepID=A0A... [more]
UPI001E1D7C3A6.040e-4360.94iduronate 2-sulfatase-like isoform X1 n=3 Tax=Merc... [more]
A0A834BU08_ORYME8.630e-4346.29Iduronate 2-sulfatase n=1 Tax=Oryzias melastigma T... [more]
A0A8C9KC90_PANTA1.890e-4245.14Iduronate 2-sulfatase n=1 Tax=Panthera tigris alta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig82646contigM-pyrifera_M_contig82646:407..898 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMammalia
Seed ortholog score146.0
Seed ortholog evalue1.5e-32
Seed eggNOG ortholog10036.XP_005085947.1
Preferred nameIDS
KEGG koko:K01136
KEGG ReactionR07812,R07821
KEGG Pathwayko00531,ko01100,ko04142,map00531,map01100,map04142
KEGG ModuleM00076,M00078
Hectar predicted targeting categoryother localisation
GOsGO:0000323,GO:0003674,GO:0003824,GO:0004423,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005773,GO:0005775,GO:0006022,GO:0006026,GO:0006027,GO:0006029,GO:0006082,GO:0006790,GO:0006807,GO:0008150,GO:0008152,GO:0008484,GO:0009056,GO:0009057,GO:0009100,GO:0009987,GO:0016787,GO:0016788,GO:0019538,GO:0030203,GO:0030204,GO:0030207,GO:0031974,GO:0043170,GO:0043202,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043436,GO:0044237,GO:0044238,GO:0044248,GO:0044260,GO:0044273,GO:0044281,GO:0044422,GO:0044424,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0050654,GO:0070013,GO:0071704,GO:1901135,GO:1901136,GO:1901564,GO:1901565,GO:1901575,GO:1903510
EggNOG free text desc.Sulfatase
EggNOG OGs35JBY@314146,38CRA@33154,3BGPB@33208,3CTVU@33213,3JEM7@40674,48564@7711,48XC5@7742,4PYB4@9989,COG3119@1,KOG3867@2759
EC3.1.6.13
COG Functional cat.P
Best tax levelRodentia
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Exons1
Model size492
Cds size471
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816733.380133-UTR-M-pyrifera_M_contig82646:406..4271622816733.380133-UTR-M-pyrifera_M_contig82646:406..427Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig82646 407..427 +
1692278146.4537206-UTR-M-pyrifera_M_contig82646:406..4271692278146.4537206-UTR-M-pyrifera_M_contig82646:406..427Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig82646 407..427 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816733.3921878-CDS-M-pyrifera_M_contig82646:427..8981622816733.3921878-CDS-M-pyrifera_M_contig82646:427..898Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82646 428..898 +
1692278146.4677942-CDS-M-pyrifera_M_contig82646:427..8981692278146.4677942-CDS-M-pyrifera_M_contig82646:427..898Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82646 428..898 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig82646.19320.1prot_M-pyrifera_M_contig82646.19320.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig82646 428..898 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig82646.19320.1

>prot_M-pyrifera_M_contig82646.19320.1 ID=prot_M-pyrifera_M_contig82646.19320.1|Name=mRNA_M-pyrifera_M_contig82646.19320.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=157bp
MRNEIGCYGVEAMHTPNMDAFAADPDTVTFERAYVQQAICNPSRTSFLTS
RRPDTTRVYDLVHYWRNVGGNFTTIPEHFKLSGYTTAGAGKVFHPLGQSG
HLDDEPYSWSPQYPYFHASAESNQKYANFSHCFYNITDVSDDQLPDGEIT
QHTLSTL
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mRNA from alignment at M-pyrifera_M_contig82646:407..898+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig82646.19320.1 ID=mRNA_M-pyrifera_M_contig82646.19320.1|Name=mRNA_M-pyrifera_M_contig82646.19320.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=492bp|location=Sequence derived from alignment at M-pyrifera_M_contig82646:407..898+ (Macrocystis pyrifera P11B4 male)
TTGTTTCTGGTTGTGGATGATATGAGGAACGAAATAGGGTGCTATGGGGT AGAAGCGATGCACACCCCGAATATGGATGCATTTGCTGCAGATCCAGACA CTGTGACGTTCGAACGAGCCTATGTACAACAAGCCATTTGTAATCCAAGT CGTACAAGCTTCCTGACTTCACGAAGACCTGACACCACCCGCGTCTACGA CCTCGTGCACTATTGGAGGAATGTTGGAGGCAATTTTACCACCATTCCAG AGCACTTCAAACTCAGTGGTTACACAACAGCTGGAGCAGGAAAGGTGTTC CATCCACTAGGACAGTCTGGTCACTTGGATGATGAGCCATACTCGTGGTC GCCTCAATATCCCTACTTCCACGCCTCTGCTGAAAGCAACCAAAAGTACG CAAACTTTTCCCACTGTTTCTACAACATCACAGATGTATCTGATGATCAA CTCCCTGATGGAGAAATCACGCAACACACTCTGAGTACACTT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig82646:407..898+

>mRNA_M-pyrifera_M_contig82646.19320.1 ID=mRNA_M-pyrifera_M_contig82646.19320.1|Name=mRNA_M-pyrifera_M_contig82646.19320.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=942bp|location=Sequence derived from alignment at M-pyrifera_M_contig82646:407..898+ (Macrocystis pyrifera P11B4 male)
ATGAGGAACGAAATAGGGTGCTATGGGGTAGAAGCGATGCACACCCCGAA
TATGGATGCATTTGCTGCAGATCCAGACACTGTGACGTTCGAACGAGCCT
ATGTACAACAAGCCATTTGTAATCCAAGTCGTACAAGCTTCCTGACTTCA
CGAAGACCTGACACCACCCGCGTCTACGACCTCGTGCACTATTGGAGGAA
TGTTGGAGGCAATTTTACCACCATTCCAGAGCACTTCAAACTCAGTGGTT
ACACAACAGCTGGAGCAGGAAAGGTGTTCCATCCACTAGGACAGTCTGGT
CACTTGGATGATGAGCCATACTCGTGGTCGCCTCAATATCCCTACTTCCA
CGCCTCTGCTGAAAGCAACCAAAAGTACGCAAACTTTTCCCACTGTTTCT
ACAACATCACAGATGTATCTGATGATCAACTCCCTGATGGAGAAATCACG
CAACACACTCTGAGTACACTTATGAGGAACGAAATAGGGTGCTATGGGGT
AGAAGCGATGCACACCCCGAATATGGATGCATTTGCTGCAGATCCAGACA
CTGTGACGTTCGAACGAGCCTATGTACAACAAGCCATTTGTAATCCAAGT
CGTACAAGCTTCCTGACTTCACGAAGACCTGACACCACCCGCGTCTACGA
CCTCGTGCACTATTGGAGGAATGTTGGAGGCAATTTTACCACCATTCCAG
AGCACTTCAAACTCAGTGGTTACACAACAGCTGGAGCAGGAAAGGTGTTC
CATCCACTAGGACAGTCTGGTCACTTGGATGATGAGCCATACTCGTGGTC
GCCTCAATATCCCTACTTCCACGCCTCTGCTGAAAGCAACCAAAAGTACG
CAAACTTTTCCCACTGTTTCTACAACATCACAGATGTATCTGATGATCAA
CTCCCTGATGGAGAAATCACGCAACACACTCTGAGTACACTT
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