mRNA_M-pyrifera_M_contig81080.18966.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig81080.18966.1
Unique NamemRNA_M-pyrifera_M_contig81080.18966.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig81080.18966.1 vs. uniprot
Match: A0A5A8DE27_CAFRO (Uncharacterized protein n=2 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DE27_CAFRO)

HSP 1 Score: 188 bits (478), Expect = 6.990e-54
Identity = 91/158 (57.59%), Postives = 106/158 (67.09%), Query Frame = 1
Query:    7 DADSNCNGIFGVDGD-GKSYEETFCSGENAPMGIAILGDSATAHFSLPPQYLNARRFPALDNLTHALELLSNEADWPQCSWSTGFRSTEGCPLSTVPMASLYQRLFEQNHCIHRDFQNLGVNGARADSLANPIEQSLARSQTKDVPLLVFVAVIGNDV 477
            D D NCNGI G D   G SYEE FCSG NAPMG+  +GDSA AHF +PPQYLNA  F    +L H +   SNE DWPQCSWSTG+R T+ CP S V M S+YQR+  +N C+HRDF N GVNGAR  S A  +  +L R    D P+L F A+IGNDV
Sbjct:  252 DVDHNCNGISGTDPQTGASYEELFCSGPNAPMGVIGIGDSALAHFHIPPQYLNAMNF----SLDHVINAASNELDWPQCSWSTGYRKTDECPASPVEMGSIYQRMRARNLCMHRDFTNAGVNGARVSSAAEVLIPNLKRDPVNDNPVLAFYALIGNDV 405          
BLAST of mRNA_M-pyrifera_M_contig81080.18966.1 vs. uniprot
Match: A0A812WFF9_9DINO (AOAH protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A812WFF9_9DINO)

HSP 1 Score: 179 bits (455), Expect = 3.870e-50
Identity = 92/162 (56.79%), Postives = 108/162 (66.67%), Query Frame = 1
Query:   13 DSNCNGIFGVDGDGKSYEETFCSGENAPMGIAILGDSATAHFSLPPQYLNARRFPALDNLTHALELLSNEADWPQCSWSTGFRSTEGCP-----LSTVPMASLYQRLFEQNHCIHRDFQNLGVNGARADSLANP--IEQSLARSQTKDVPLLVFVAVIGNDV 477
            D NCNGI GV+  G  YEE +CSG NAPMG+ ILGDSATAHF +PPQY+NA+ F     L   +   +NEADWPQCSWSTGFR +  CP     L+     S+Y  +   N C HRD QN GVNGAR  S+A P  I  SLAR+QT D P+LVF A+IGNDV
Sbjct:  320 DHNCNGISGVNASGIPYEEAWCSGANAPMGVGILGDSATAHFHIPPQYINAQNFSL--PLDRIIAFAANEADWPQCSWSTGFRDSVDCPATVDNLTMANYPSIYGYMNRLNKCNHRDLQNTGVNGARVGSVAAPDGIMYSLARNQTMDHPMLVFWALIGNDV 479          
BLAST of mRNA_M-pyrifera_M_contig81080.18966.1 vs. uniprot
Match: A0A0S4JM56_BODSA (GPI inositol deacylase, putative n=1 Tax=Bodo saltans TaxID=75058 RepID=A0A0S4JM56_BODSA)

HSP 1 Score: 157 bits (397), Expect = 1.830e-42
Identity = 84/158 (53.16%), Postives = 105/158 (66.46%), Query Frame = 1
Query:   13 DSNCNGIFGVDG-DGKSYEETFCSGENAPMGIAILGDSATAHFSLPPQYLNARRFPALDNLTHALELLSNEADWPQCSWSTG-FRSTEGCPLSTVPMASLYQRLFEQNHCIHRDFQNLGVNGARADSLANPIEQSLARSQTKDV-PLLVFVAVIGNDV 477
            D NCNGI+GVD   G+ YE+ +C+G NA  G+A LGDSATAHF LPP YL A    +  N  H L LL NEADWP  SWSTG   STE  P    P++SLY R+ E N C HRD+QNLGVNGAR   L N  +  L+R++  +V PL++  +++GNDV
Sbjct:  201 DENCNGIYGVDALTGQPYEDKWCAGTNA-FGVASLGDSATAHFRLPPNYLVAANLSSA-NFAHILRLLENEADWPMLSWSTGHLNSTEFNPDIYGPVSSLYSRMLEHNRCNHRDYQNLGVNGARVSDLIN-FDTELSRNRKDNVKPLVMIYSMVGNDV 355          
BLAST of mRNA_M-pyrifera_M_contig81080.18966.1 vs. uniprot
Match: F6T5V5_CIOIN (Saposin B-type domain-containing protein n=2 Tax=Ciona intestinalis TaxID=7719 RepID=F6T5V5_CIOIN)

HSP 1 Score: 154 bits (390), Expect = 2.190e-42
Identity = 79/160 (49.38%), Postives = 106/160 (66.25%), Query Frame = 1
Query:    1 DVDADSNCNGIFGVDGD-GKSYEETFCSGENAPMGIAILGDSATAHFSLPPQYLNARRFPALDNLTHALELLSNEADWPQCSWSTGFRSTEGCPLSTVPMASLYQRLFEQNHCIHRDFQNLGVNGARADSLANPIEQSLARSQTKDVPLLVFVAVIGNDV 477
            D+  DSNCNGI+G D   G+ YEE +C+  N P G+ ILGDSA AHF LPP+Y  A      D     L LL++E DWP+ S  T F S E       P+ S+Y +L+++N CIHRDFQN+GVNGAR+ S+ + I +SLAR+ T+D PL + +A++GNDV
Sbjct:  211 DITYDSNCNGIYGADPQTGRPYEELYCANTN-PKGVVILGDSAAAHFHLPPEYFMAEVLNK-DTFKDVLMLLTDEFDWPELSAITAFTSNEWPESIDGPVDSIYMKLYDRNRCIHRDFQNIGVNGARSGSMTD-IVKSLARNATQDYPLFIVLALVGNDV 367          
BLAST of mRNA_M-pyrifera_M_contig81080.18966.1 vs. uniprot
Match: A0A7S1CFD0_9STRA (Hypothetical protein n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CFD0_9STRA)

HSP 1 Score: 152 bits (384), Expect = 1.110e-41
Identity = 80/165 (48.48%), Postives = 103/165 (62.42%), Query Frame = 1
Query:    1 DVDADSNCNGIFGVDGD-GKSYEETFCSGENAPMGIAILGDSATAHFSLPPQYLNARRFPALDNLTHALELLSNEADWPQCSWSTGFRSTEGCPLSTVPMASLYQRLFEQNHCIHRDFQNLGVNGARA-----DSLANPIEQSLARSQTKDVPLLVFVAVIGNDV 477
            D + D NCNGI GVD   GKSYEE +C G++   GI ILGDSA AHF++PP YL  +++    +L   +    +EADWPQCSW+TG+ ++  C  S +PM S+YQRL   N C HRDF N+GVNGAR      D     I +S  R Q  D P++   A+IGNDV
Sbjct:   37 DPNIDHNCNGISGVDPTTGKSYEEMWCKGQDT-RGITILGDSAAAHFNIPPSYLRHQKW----SLKGLVPFAEDEADWPQCSWATGYVNSSDCLPSALPMKSIYQRLRNHNLCSHRDFANIGVNGARTGAMMPDGHGGGIIESFQRDQQHDNPVVTVFALIGNDV 196          
BLAST of mRNA_M-pyrifera_M_contig81080.18966.1 vs. uniprot
Match: UPI001425A3C9 (acyloxyacyl hydrolase-like isoform X2 n=1 Tax=Anneissia japonica TaxID=1529436 RepID=UPI001425A3C9)

HSP 1 Score: 149 bits (377), Expect = 3.010e-40
Identity = 79/160 (49.38%), Postives = 99/160 (61.88%), Query Frame = 1
Query:    1 DVDADSNCNGIFGVDG-DGKSYEETFCSGENAPMGIAILGDSATAHFSLPPQYLNARRFPALDNLTHALELLSNEADWPQCSWSTGFRSTEGCPLSTVPMASLYQRLFEQNHCIHRDFQNLGVNGARADSLANPIEQSLARSQTKDVPLLVFVAVIGNDV 477
            D+  DSNCNGI GVD   GKSYEE FC G   PMG+AILGDSATAHF +P  +         D       ++ NE DWPQ S+STGF +T        P  SLY R+ ++N C HRD+QN+GVNGA   S+   ++ SLAR Q  D PL+V  +++GNDV
Sbjct:   88 DIVEDSNCNGIMGVDPKSGKSYEELFCKGSR-PMGLAILGDSATAHFHIPVNWFTPAEINK-DIFKEMPMVIENEFDWPQVSYSTGFMNTSTWKTIEGPTDSLYLRMRQRNLCNHRDYQNIGVNGATTVSMNRTVQYSLARDQKADKPLVVIYSLLGNDV 245          
BLAST of mRNA_M-pyrifera_M_contig81080.18966.1 vs. uniprot
Match: UPI001B889F95 (acyloxyacyl hydrolase-like n=1 Tax=Gigantopelta aegis TaxID=1735272 RepID=UPI001B889F95)

HSP 1 Score: 150 bits (380), Expect = 5.140e-40
Identity = 74/160 (46.25%), Postives = 104/160 (65.00%), Query Frame = 1
Query:    1 DVDADSNCNGIFGVD-GDGKSYEETFCSGENAPMGIAILGDSATAHFSLPPQYLNARRFPALDNLTHALELLSNEADWPQCSWSTGFRSTEGCPLSTVPMASLYQRLFEQNHCIHRDFQNLGVNGARADSLANPIEQSLARSQTKDVPLLVFVAVIGNDV 477
            D+  DSNCNGI+G D    + YEE FC G   P G+ +LGDS +AHF LP ++L++R+  +     H   ++ NE DWP+ S  TGF +     +   PM S+Y+R+FE+N C HRD+QN+ VNGAR+ S+A+ I  SL R+  KD P+ VF A++GNDV
Sbjct:  190 DITDDSNCNGIYGSDPASTRPYEEVFCEGTE-PRGVVVLGDSISAHFHLPEEWLDSRKL-SKKVFQHLPFIIENEIDWPELSSITGFANNSWPEVIQGPMDSIYKRMFERNRCNHRDYQNIAVNGARSSSMADTIMYSLKRNGEKDYPVFVFYALVGNDV 347          
BLAST of mRNA_M-pyrifera_M_contig81080.18966.1 vs. uniprot
Match: F2TZY4_SALR5 (Acyloxyacyl hydrolase n=1 Tax=Salpingoeca rosetta (strain ATCC 50818 / BSB-021) TaxID=946362 RepID=F2TZY4_SALR5)

HSP 1 Score: 151 bits (381), Expect = 5.280e-40
Identity = 82/163 (50.31%), Postives = 107/163 (65.64%), Query Frame = 1
Query:    1 DVDADSNCNGIFGVDG-DGKSYEETFCSGENAPMGIAILGDSATAHFSLPPQYLNARRFPALDNLTHA--LELLSNEADWPQCSWSTGFRST-EGCPLSTVPMASLYQRLFEQNHCIHRDFQNLGVNGARADSLANPIEQSLARSQTKDVPLLVFVAVIGNDV 477
            D + DSNCNGI+G++   GK+YEE +C+  N PMG  +LGDSA+AHF +P QYL+   +   DN T A  L  L NE DWP  S +TGF +T +  P  T    S YQ ++ +N C+HRDFQN+ VNGARA S+ + I QS++R Q  D P LV  A+IGNDV
Sbjct:  225 DREVDSNCNGIYGIEPKSGKTYEELYCADTN-PMGTIVLGDSASAHFHIPYQYLSVVDW---DNTTFANILPTLENEFDWPMLSATTGFENTTQWAPDVTGKQNSTYQVMWRRNRCVHRDFQNIAVNGARAGSMNSSIVQSMSRLQKSDYPALVMYALIGNDV 383          
BLAST of mRNA_M-pyrifera_M_contig81080.18966.1 vs. uniprot
Match: UPI0014259467 (acyloxyacyl hydrolase-like n=1 Tax=Anneissia japonica TaxID=1529436 RepID=UPI0014259467)

HSP 1 Score: 149 bits (376), Expect = 2.380e-39
Identity = 79/160 (49.38%), Postives = 99/160 (61.88%), Query Frame = 1
Query:    1 DVDADSNCNGIFGVDG-DGKSYEETFCSGENAPMGIAILGDSATAHFSLPPQYLNARRFPALDNLTHALELLSNEADWPQCSWSTGFRSTEGCPLSTVPMASLYQRLFEQNHCIHRDFQNLGVNGARADSLANPIEQSLARSQTKDVPLLVFVAVIGNDV 477
            D+  DSNCNGI GVD   GKSYEE FC G   PMG+AILGDSATAHF +P  +         D       ++ NE DWPQ S+STGF +T        P  SLY R+ ++N C HRD+QN+GVNGA   S+   ++ SLAR Q  D PL+V  +++GNDV
Sbjct:  213 DIVEDSNCNGIMGVDPKSGKSYEELFCKGSR-PMGLAILGDSATAHFHIPVNWFTPAEINK-DIFKEMPMVIDNEFDWPQVSYSTGFMNTSTWKTIEGPTDSLYLRMRQRNLCNHRDYQNIGVNGATTVSMNRTVQYSLARDQKADNPLVVIYSLLGNDV 370          
BLAST of mRNA_M-pyrifera_M_contig81080.18966.1 vs. uniprot
Match: A0A820H1N5_9BILA (Hypothetical protein (Fragment) n=1 Tax=Adineta steineri TaxID=433720 RepID=A0A820H1N5_9BILA)

HSP 1 Score: 144 bits (364), Expect = 3.270e-39
Identity = 77/156 (49.36%), Postives = 104/156 (66.67%), Query Frame = 1
Query:   13 DSNCNGIFGVDG-DGKSYEETFCSGENAPMGIAILGDSATAHFSLPPQYLNARRFPALDNLTHALELLSNEADWPQCSWSTGFRSTEGCPLSTVPMASLYQRLFEQNHCIHRDFQNLGVNGARADSLANPIEQSLARSQTKDVPLLVFVAVIGNDV 477
            D NCNGIFG++   G+ +E+ FC+ E   MGIA+LGDS +AHF +P Q+L+A +F   +   H   +L NE DWPQ S STG  +    P    P  SLY RLF+ +HC HRD+QN+ VNGAR+ S+ N I ++L+R Q  DVPLLV  +++GNDV
Sbjct:  190 DHNCNGIFGMNSATGRPFEDEFCN-ETQRMGIAVLGDSLSAHFHIPEQWLDASQFSP-EAFEHLGFILENELDWPQLSASTGHVNVSW-PNIIGPTRSLYARLFDLDHCNHRDYQNIAVNGARSSSMTN-IMKTLSRHQKNDVPLLVIYSLVGNDV 341          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig81080.18966.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8DE27_CAFRO6.990e-5457.59Uncharacterized protein n=2 Tax=Cafeteria roenberg... [more]
A0A812WFF9_9DINO3.870e-5056.79AOAH protein n=1 Tax=Symbiodinium sp. KB8 TaxID=23... [more]
A0A0S4JM56_BODSA1.830e-4253.16GPI inositol deacylase, putative n=1 Tax=Bodo salt... [more]
F6T5V5_CIOIN2.190e-4249.38Saposin B-type domain-containing protein n=2 Tax=C... [more]
A0A7S1CFD0_9STRA1.110e-4148.48Hypothetical protein n=1 Tax=Bicosoecida sp. CB-20... [more]
UPI001425A3C93.010e-4049.38acyloxyacyl hydrolase-like isoform X2 n=1 Tax=Anne... [more]
UPI001B889F955.140e-4046.25acyloxyacyl hydrolase-like n=1 Tax=Gigantopelta ae... [more]
F2TZY4_SALR55.280e-4050.31Acyloxyacyl hydrolase n=1 Tax=Salpingoeca rosetta ... [more]
UPI00142594672.380e-3949.38acyloxyacyl hydrolase-like n=1 Tax=Anneissia japon... [more]
A0A820H1N5_9BILA3.270e-3949.36Hypothetical protein (Fragment) n=1 Tax=Adineta st... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig81080contigM-pyrifera_M_contig81080:1..480 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeOpisthokonta
Seed ortholog score150.2
Seed ortholog evalue8.1e-34
Seed eggNOG ortholog946362.XP_004997273.1
Preferred nameAOAH
KEGG koko:K01065
Hectar predicted targeting categoryother localisation
GOsGO:0000272,GO:0003674,GO:0003824,GO:0005488,GO:0005509,GO:0005975,GO:0005976,GO:0006082,GO:0006629,GO:0006631,GO:0008150,GO:0008152,GO:0008653,GO:0009056,GO:0009057,GO:0009104,GO:0009987,GO:0016042,GO:0016052,GO:0016787,GO:0016788,GO:0019752,GO:0031347,GO:0031348,GO:0032101,GO:0032102,GO:0032787,GO:0043167,GO:0043169,GO:0043170,GO:0043436,GO:0044237,GO:0044238,GO:0044242,GO:0044247,GO:0044248,GO:0044255,GO:0044260,GO:0044262,GO:0044264,GO:0044275,GO:0044281,GO:0046872,GO:0048519,GO:0048583,GO:0048585,GO:0050528,GO:0050727,GO:0050728,GO:0050789,GO:0052689,GO:0065007,GO:0071704,GO:0080134,GO:1901135,GO:1901136,GO:1901575,GO:1903509
EggNOG free text desc.Acyloxyacyl hydrolase
EggNOG OGs28P48@1,2QVQW@2759,39S84@33154
EC3.1.1.77
COG Functional cat.S
Best tax levelOpisthokonta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000
Exons1
Model size480
Cds size480
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816716.5093362-CDS-M-pyrifera_M_contig81080:0..4801622816716.5093362-CDS-M-pyrifera_M_contig81080:0..480Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81080 1..480 +
1692278137.1034994-CDS-M-pyrifera_M_contig81080:0..4801692278137.1034994-CDS-M-pyrifera_M_contig81080:0..480Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81080 1..480 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig81080.18966.1prot_M-pyrifera_M_contig81080.18966.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig81080 1..480 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig81080.18966.1

>prot_M-pyrifera_M_contig81080.18966.1 ID=prot_M-pyrifera_M_contig81080.18966.1|Name=mRNA_M-pyrifera_M_contig81080.18966.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=160bp
DVDADSNCNGIFGVDGDGKSYEETFCSGENAPMGIAILGDSATAHFSLPP
QYLNARRFPALDNLTHALELLSNEADWPQCSWSTGFRSTEGCPLSTVPMA
SLYQRLFEQNHCIHRDFQNLGVNGARADSLANPIEQSLARSQTKDVPLLV
FVAVIGNDV*
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mRNA from alignment at M-pyrifera_M_contig81080:1..480+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig81080.18966.1 ID=mRNA_M-pyrifera_M_contig81080.18966.1|Name=mRNA_M-pyrifera_M_contig81080.18966.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=480bp|location=Sequence derived from alignment at M-pyrifera_M_contig81080:1..480+ (Macrocystis pyrifera P11B4 male)
GACGTGGATGCGGACTCGAACTGCAACGGCATTTTCGGTGTGGACGGCGA CGGCAAGTCGTACGAAGAGACGTTTTGCAGCGGCGAGAACGCTCCCATGG GCATCGCCATCTTGGGAGACTCTGCCACGGCGCATTTCTCGCTGCCGCCA CAGTACTTGAACGCGCGCCGCTTTCCTGCGCTGGACAACCTCACCCACGC CCTTGAGCTCCTCTCCAACGAGGCCGATTGGCCACAGTGCTCATGGTCCA CGGGCTTCCGATCCACCGAGGGCTGCCCGCTCTCCACCGTCCCCATGGCA TCGCTCTACCAACGGCTCTTTGAACAAAACCACTGCATCCACCGAGACTT CCAAAACCTCGGAGTCAACGGAGCAAGGGCAGACTCGCTCGCCAACCCCA TCGAACAGTCCCTCGCCAGGTCGCAAACGAAAGACGTCCCGCTCCTCGTC TTCGTCGCTGTCATCGGAAACGACGTGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig81080:1..480+

>mRNA_M-pyrifera_M_contig81080.18966.1 ID=mRNA_M-pyrifera_M_contig81080.18966.1|Name=mRNA_M-pyrifera_M_contig81080.18966.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=960bp|location=Sequence derived from alignment at M-pyrifera_M_contig81080:1..480+ (Macrocystis pyrifera P11B4 male)
GACGTGGATGCGGACTCGAACTGCAACGGCATTTTCGGTGTGGACGGCGA
CGGCAAGTCGTACGAAGAGACGTTTTGCAGCGGCGAGAACGCTCCCATGG
GCATCGCCATCTTGGGAGACTCTGCCACGGCGCATTTCTCGCTGCCGCCA
CAGTACTTGAACGCGCGCCGCTTTCCTGCGCTGGACAACCTCACCCACGC
CCTTGAGCTCCTCTCCAACGAGGCCGATTGGCCACAGTGCTCATGGTCCA
CGGGCTTCCGATCCACCGAGGGCTGCCCGCTCTCCACCGTCCCCATGGCA
TCGCTCTACCAACGGCTCTTTGAACAAAACCACTGCATCCACCGAGACTT
CCAAAACCTCGGAGTCAACGGAGCAAGGGCAGACTCGCTCGCCAACCCCA
TCGAACAGTCCCTCGCCAGGTCGCAAACGAAAGACGTCCCGCTCCTCGTC
TTCGTCGCTGTCATCGGAAACGACGTGTAAGACGTGGATGCGGACTCGAA
CTGCAACGGCATTTTCGGTGTGGACGGCGACGGCAAGTCGTACGAAGAGA
CGTTTTGCAGCGGCGAGAACGCTCCCATGGGCATCGCCATCTTGGGAGAC
TCTGCCACGGCGCATTTCTCGCTGCCGCCACAGTACTTGAACGCGCGCCG
CTTTCCTGCGCTGGACAACCTCACCCACGCCCTTGAGCTCCTCTCCAACG
AGGCCGATTGGCCACAGTGCTCATGGTCCACGGGCTTCCGATCCACCGAG
GGCTGCCCGCTCTCCACCGTCCCCATGGCATCGCTCTACCAACGGCTCTT
TGAACAAAACCACTGCATCCACCGAGACTTCCAAAACCTCGGAGTCAACG
GAGCAAGGGCAGACTCGCTCGCCAACCCCATCGAACAGTCCCTCGCCAGG
TCGCAAACGAAAGACGTCCCGCTCCTCGTCTTCGTCGCTGTCATCGGAAA
CGACGTGTAA
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