mRNA_M-pyrifera_M_contig80788.18901.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig80788.18901.1
Unique NamemRNA_M-pyrifera_M_contig80788.18901.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig80788.18901.1 vs. uniprot
Match: A0A6A5BPP9_NAEFO (Uncharacterized protein n=1 Tax=Naegleria fowleri TaxID=5763 RepID=A0A6A5BPP9_NAEFO)

HSP 1 Score: 120 bits (300), Expect = 2.540e-28
Identity = 72/200 (36.00%), Postives = 107/200 (53.50%), Query Frame = 1
Query:    1 MPIRRGRTVRLTPGQAAANEACSRFLLKFIVGLFLIAVPLGLCWAEYEQREMHEAFEEAKQQAISVTPEAPVSQM----NVGSLV--HVSTRDVRTEETLVDEDFAVQGPPNALKLQRKSQYCQWIE--HVSEKKNRDGD--VVRSYWYTKSWVPVPVPSLFFDQPFRYNNPQRAPYANGALAVSEAQLGSYTIGPRLID 570
            +  R GR+  ++  Q      C+ + LK I  +FL+A PL L +AE E   M  AF E  +   ++    P++      N   L+  HV + ++RTE  + D DF +     A ++ RK++YCQWIE  H  EK   DG    VR+Y+Y + WV   +PSLFFDQP  ++NPQR P+ +    V    LG + +GP LI+
Sbjct:   22 LTTRVGRSRIISDTQLKIENTCALWFLKIIFAIFLVAAPLTLFYAENEAMLMQHAFLEVVRSTRTIVD--PINSKPRPENNNQLIFFHVPSHNIRTEHPIQDRDFGLTFN-GAFEVNRKTEYCQWIESHHDEEKTRSDGSKYTVRTYYYNRGWVDHLIPSLFFDQPVAHHNPQRDPFPSVTQKVEGVTLGKFHLGPNLIE 218          
BLAST of mRNA_M-pyrifera_M_contig80788.18901.1 vs. uniprot
Match: A0A1Y2CNH3_9FUNG (Uncharacterized protein n=1 Tax=Rhizoclosmatium globosum TaxID=329046 RepID=A0A1Y2CNH3_9FUNG)

HSP 1 Score: 99.0 bits (245), Expect = 1.110e-20
Identity = 58/176 (32.95%), Postives = 95/176 (53.98%), Query Frame = 1
Query:   82 KFIVGLF----LIAVPLGLCWAEYEQREMHEAFEEAKQQAISVTPEAPVSQMNVGSLVHVSTRDVRTEETLVDEDFAVQGPPNALKLQRKSQYCQWIEHVSE----KKNRDGD----VVRSYWYTKSWVPVPVPSLFFDQPFRYNNPQRAPYANGALAVSEAQLGSYTIGPRLIDS 573
            +F+  +F    +++VPL L + E  Q E   A  EA    + V P  P ++  VGSLV  S+  V+ +  + D+ F +  P NA++++R ++YCQW E   +    +++ DG+       +Y+Y K W   P+ S FFDQP  + NPQR PY +     S+  LG YT+ P ++ +
Sbjct:   28 RFVTTVFKLAAIVSVPLVLAYLEQSQAETQAAVAEA---LVKVVPATPTTK--VGSLVFASSHSVQPD-LISDQSFGLDFP-NAVRVKRSTEYCQWQEFTQQDSRTERDEDGNEHTVTTTTYYYNKGWYSYPINSFFFDQPAAHYNPQRDPYPSKTQTTSKLTLGEYTVRPSVLQN 196          
BLAST of mRNA_M-pyrifera_M_contig80788.18901.1 vs. uniprot
Match: A0A7S4N4X2_9EUKA (Hypothetical protein n=1 Tax=Paramoeba aestuarina TaxID=180227 RepID=A0A7S4N4X2_9EUKA)

HSP 1 Score: 81.6 bits (200), Expect = 1.690e-14
Identity = 62/202 (30.69%), Postives = 99/202 (49.01%), Query Frame = 1
Query:   10 RRGRTVRLTPGQAAANEACSRFLLKFIVGLFLIAVPLGLCWAEYEQREMHEAFEEAKQQAISVTPEAPVSQMNVGSLVHVSTRDVRTEETLVDEDFAVQGPPNALKLQRKSQYCQWIEHVSEK------KNRDG-------DVVRSYWYTKSWVPVPVPSLFFDQPFRYNNPQRAPYANGALAVSEAQLGSYTIGPRLIDSL 576
            R+GRT  + PG+   + +     L  +  +FLIA PL L +AE+    +  +  E     I V     V   +    +H +T  +  +  +VDE F V+  P AL L RK++YCQW+EH  E+      +  DG       + +  Y YTK W    + SL FDQP  + NPQR P+ +       A++  +++ P L+++L
Sbjct:   18 RQGRTYYV-PGKK--DTSAQTICLSILFVIFLIACPLILFYAEFNFALLVASITEVAPDVIDVDNPHQVP-WHGKKYIH-TTGMINYDAEVVDEAFHVKVNP-ALILNRKTEYCQWMEHRHEECQTCSEEGEDGKTHTYQCNCIIRYEYTKQWSSFRINSLLFDQPGAHYNPQRDPFPSQKFVSQNAEVNGFSLKPALVNNL 213          
BLAST of mRNA_M-pyrifera_M_contig80788.18901.1 vs. uniprot
Match: A0A0G4HQE8_9ALVE (Uncharacterized protein (Fragment) n=1 Tax=Chromera velia CCMP2878 TaxID=1169474 RepID=A0A0G4HQE8_9ALVE)

HSP 1 Score: 79.3 bits (194), Expect = 5.300e-14
Identity = 67/203 (33.00%), Postives = 94/203 (46.31%), Query Frame = 1
Query:   40 GQAAANEACSRFLLKFIVGLFLIAVPLGLCWAEYEQREMHEAFEEAKQQAISVT-PEAPVSQM------NVGSLVHVSTRDVRTEETLVDEDFAVQGPPNALKLQRKSQYCQWIEHVSEK--KNRDGDV---VRSYWYTKSWVPVPVPSLFFDQPFRYNNPQRAPYANGALAVSEAQLGSYTIGPRLIDSLP-VAEPVRLETF 609
            G+A +N  CS F     + + L A+ L   W E     ++ A  E    A+  + PE     +      + GSL  V       E +  D DF V+ P  ALK+ RK++YCQW E  SE   KN D D    V SY Y K+W    + SL FDQP  ++NPQR P+ +       A++    I P ++  L  V  PV  +T 
Sbjct:   31 GRAGSNPCCSYFCF---IAVTLAALGLS-AWREITFFHINSALNEVGDAAVDCSGPETGFESLVECGKRSAGSLNAVFFSGP-VEASASDPDFGVEVP-GALKVHRKTEYCQWEEIRSESCTKNDDEDTETCVTSYSYVKAWRSYRINSLLFDQPGAHHNPQRDPFPSTYFPSQNAKIRDVPIDPEVLTRLQGVWHPVEWDTM 227          
BLAST of mRNA_M-pyrifera_M_contig80788.18901.1 vs. uniprot
Match: A0A2R4KHG2_9BACT (Uncharacterized protein n=5 Tax=Tannerella serpentiformis TaxID=712710 RepID=A0A2R4KHG2_9BACT)

HSP 1 Score: 67.4 bits (163), Expect = 1.200e-9
Identity = 64/208 (30.77%), Postives = 93/208 (44.71%), Query Frame = 1
Query:   22 TVRLTPGQAAANEACSRF--LLKFIVGLFLIAVPLGLCWAEYEQREMHEAFEEAKQQAISVTPEAPVSQMNVGSLVHVSTRDVRTEETLVDEDFAVQGPPNALKLQRKSQYCQWIEHVSEKKNRDG-----DVVRSYWYTKSWVPVPVPSLFFDQPFRYNNPQRAPYANGALAVSEAQ--------LGSYTIGPRLIDSLPVAEPVRL 600
            T   + GQ  A  A      L+ FI+G  L      L W E       +A  +A     SV   + V     G L+H S     T++TL D+ F V+    A+KL RK +Y QW+EH S++K RD      + + +Y Y + WV  PV S  F +  RY N      AN  L+  E +         G+YT+    + S+  +EPV +
Sbjct:    6 TTSTSYGQRLAGSAKGMIGGLMMFIIGTCL------LWWNEGRTVRTAKAIGDAASHVESVADVSRVDASLNGKLIHASAF-ADTKDTLTDDLFGVRE--QAIKLDRKVEYYQWVEH-SQRKKRDKVGGGEETITTYTYEQEWVKKPVNSSNFKES-RYRN------ANRVLSEVEERNEMAQHVTFGAYTLPESFVASISGSEPVEV 196          
BLAST of mRNA_M-pyrifera_M_contig80788.18901.1 vs. uniprot
Match: UPI000C04EDC7 (transmembrane protein 43-like n=1 Tax=Stylophora pistillata TaxID=50429 RepID=UPI000C04EDC7)

HSP 1 Score: 63.5 bits (153), Expect = 2.220e-8
Identity = 45/184 (24.46%), Postives = 86/184 (46.74%), Query Frame = 1
Query:   85 FIVGLFLIAVPLGLCWAEYEQREMHEAFEEAKQQAISVTPEAPVSQMNVGSLVHVSTRDVRTEETLVDEDFAVQGPPNALKLQRKSQYCQWIEHVSEKKNRDGD---VVRSYWYTKSWVPVPVPSLFFDQPFRYNNPQRAPYANGALAVSEAQLGSYTIGPRLIDSLPVAEPVRLETFPSDALV 627
            F + L +++ P+ L W E    +   + +E  +Q + +     +S      LVH+S   ++T+  L D D+ V     A+KL+RK +  QW+EH   ++  +G+   V  ++ YT  W    + S  FD P  + NP   P  +        ++G++++   LI  + V +P+     P   ++
Sbjct:   47 FGILLVIVSFPV-LYWNEGRAVQTALSLDEGLRQVVHIDYVDQISPEYQDKLVHLSG-SLQTDRALSDSDYGV--AVKAVKLRRKVEMYQWVEHKHTREYDEGERKRVETTFSYTTEWRDTIIRSDGFDNPAGHQNPNSMPVQSYIKEADSVRVGAFSLSRGLIGKIDVFQPLSPREVPHGKMI 226          
BLAST of mRNA_M-pyrifera_M_contig80788.18901.1 vs. uniprot
Match: UPI001B88930B (transmembrane protein 43-like n=1 Tax=Gigantopelta aegis TaxID=1735272 RepID=UPI001B88930B)

HSP 1 Score: 62.8 bits (151), Expect = 4.760e-8
Identity = 44/185 (23.78%), Postives = 88/185 (47.57%), Query Frame = 1
Query:   58 EACSRFLLKFIVGLFLIAVPLGLC-WAEYEQREMHEAFEEAKQQAISVTPEAPVSQMNVGSLVHVSTRDVRTEETLVDEDFAVQGPPNALKLQRKSQYCQWIEHVSEKKNRDGDVVR---SYWYTKSWVPVPVPSLFFDQPFRYNNPQRAPYANGALAVSEAQLGSYTIGPRLIDSLPVAEPVRL 600
            E     L+  +VGL ++     L  W E    +  ++ +E  +  + +         N G LVH+S   +RT++ L D  + +  P  ++KL+RK +  QW+EH ++++  +G   R   +Y Y++ W    + S  FD    + NP+     +  L   E ++G++ +   L++ +   +P+ L
Sbjct:   33 ERIGNSLVGILVGLVILTGASCLVFWNEGRAVQTAKSLDEGLRVVLPLGANEVAFDENNGKLVHISGL-LRTDKMLEDSLYKISTP--SVKLKRKVEMYQWVEHETKREYNEGGQTRVETTYSYSQEWSSDLIRSSSFDNSVSHVNPESMSVQSLTLVAHEVRVGNFFLSNSLVEKITDYKPLNL 214          
BLAST of mRNA_M-pyrifera_M_contig80788.18901.1 vs. uniprot
Match: A0A1Y4CZZ7_9DELT (Uncharacterized protein n=3 Tax=Desulfovibrio TaxID=872 RepID=A0A1Y4CZZ7_9DELT)

HSP 1 Score: 60.5 bits (145), Expect = 2.960e-7
Identity = 51/172 (29.65%), Postives = 77/172 (44.77%), Query Frame = 1
Query:   97 LFLIAVPLGLCWAEYEQREMHEAFEEAKQQAISVTPEAPVSQMNVGSLVHVSTRDVRTEETLVDEDFAVQGPPNALKLQRKSQYCQWIEHV-SEKKNRDG---DVVRSYWYTKSWVPVPVPSLFFDQPFRYNNPQRAPYANGALAVSEAQLGSYTIGPRLIDSLPVAEPVRL 600
            L +IA    L W E       +A  EA+     +     V     G +V+ + R V T++ L D  F V+   NA+KL+RK +Y QW+EH  SEK+ + G   + V +Y Y++ WV  PV S  F Q   + N  R               G+Y     L  S+   +P+ +
Sbjct:   32 LLIIAATALLWWNEGRTVRTGDAIVEAQLATEPMPAITKVDSAFEGKMVYATGRAV-TKDELTDPVFGVRV--NAIKLRRKVEYYQWVEHRRSEKRQKLGGGEETVTTYTYSREWVNHPVDSQSFKQMVGHENKTRIQTEAADWLAPNVTFGAYRFPAFLARSIGGEKPLDI 200          
BLAST of mRNA_M-pyrifera_M_contig80788.18901.1 vs. uniprot
Match: UPI00077B1F9B (LOW QUALITY PROTEIN: transmembrane protein 43-like n=2 Tax=Acropora TaxID=6127 RepID=UPI00077B1F9B)

HSP 1 Score: 60.5 bits (145), Expect = 2.970e-7
Identity = 44/164 (26.83%), Postives = 77/164 (46.95%), Query Frame = 1
Query:   88 IVGLFLIAVPLGLC-WAEYEQREMHEAFEEAKQQAISVTPEAPVSQMNVGSLVHVSTRDVRTEETLVDEDFAVQGPPNALKLQRKSQYCQWIEHVSEKKNRDGDVVR---SYWYTKSWVPVPVPSLFFDQPFRYNNPQRAPYANGALAVSEAQLGSYTIGPRLI 567
            ++G+ L+     +  W E    +   + +E  +Q  S+     ++  N   LVH+ T  ++T+  L D ++ V     A+KL+RK +  QW+EH S  +  +G   R   ++ YTK W    V S  FD+P  + NP   P  +        Q+G++ +   LI
Sbjct:   59 LMGILLVITSFPVIYWNEGRAVQTSLSLDEGLKQVFSLQVIDQINPENQNRLVHL-TGPLQTQRALSDPEYGV--AVRAVKLRRKVEMYQWVEHTSTSEFDEGPQKRIETTFSYTKEWKDHVVRSNSFDKPSGHMNPSSFPVTSYTNEADFVQVGAFVLSKGLI 219          
BLAST of mRNA_M-pyrifera_M_contig80788.18901.1 vs. uniprot
Match: UPI000F556C86 (transmembrane protein 43-like n=1 Tax=Pocillopora damicornis TaxID=46731 RepID=UPI000F556C86)

HSP 1 Score: 58.9 bits (141), Expect = 9.770e-7
Identity = 42/184 (22.83%), Postives = 82/184 (44.57%), Query Frame = 1
Query:   88 IVGLFLIAVPLGLC-WAEYEQREMHEAFEEAKQQAISVTPEAPVSQMNVGSLVHVSTRDVRTEETLVDEDFAVQGPPNALKLQRKSQYCQWIEHVSEKKNRDGD---VVRSYWYTKSWVPVPVPSLFFDQPFRYNNPQRAPYANGALAVSEAQLGSYTIGPRLIDSLPVAEPVRLETFPSDALV 627
            + G+ L+ V   +  W E    +   + +E  +Q + +      S      LVH+S   ++T+  L D D+ +     A+KL+RK +  QW+EH   ++  +G+   V  ++ Y   W  + V S  FD P  + NP   P  +        ++G +++   LI  +   +P+  +  P   ++
Sbjct:   46 LFGILLVIVSFPVIYWNEGRAVQTALSLDEGLKQVVHIHYIDQTSPEYQDKLVHLSG-SLQTDRALSDSDYGI--AVKAVKLRRKVEMYQWVEHKHTREYDEGERKRVETTFSYATEWRDIIVRSDSFDNPAGHQNPNTMPVQSYTKEADSVRVGVFSLSKGLIGKIDAFQPLSPQEVPQGKMM 226          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig80788.18901.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 13
Match NameE-valueIdentityDescription
A0A6A5BPP9_NAEFO2.540e-2836.00Uncharacterized protein n=1 Tax=Naegleria fowleri ... [more]
A0A1Y2CNH3_9FUNG1.110e-2032.95Uncharacterized protein n=1 Tax=Rhizoclosmatium gl... [more]
A0A7S4N4X2_9EUKA1.690e-1430.69Hypothetical protein n=1 Tax=Paramoeba aestuarina ... [more]
A0A0G4HQE8_9ALVE5.300e-1433.00Uncharacterized protein (Fragment) n=1 Tax=Chromer... [more]
A0A2R4KHG2_9BACT1.200e-930.77Uncharacterized protein n=5 Tax=Tannerella serpent... [more]
UPI000C04EDC72.220e-824.46transmembrane protein 43-like n=1 Tax=Stylophora p... [more]
UPI001B88930B4.760e-823.78transmembrane protein 43-like n=1 Tax=Gigantopelta... [more]
A0A1Y4CZZ7_9DELT2.960e-729.65Uncharacterized protein n=3 Tax=Desulfovibrio TaxI... [more]
UPI00077B1F9B2.970e-726.83LOW QUALITY PROTEIN: transmembrane protein 43-like... [more]
UPI000F556C869.770e-722.83transmembrane protein 43-like n=1 Tax=Pocillopora ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig80788contigM-pyrifera_M_contig80788:26..652 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score82.4
Seed ortholog evalue2.7e-13
Seed eggNOG ortholog296587.XP_002501214.1
Preferred nameTMEM43
Hectar predicted targeting categorysignal peptide
GOsGO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005635,GO:0005637,GO:0005639,GO:0005737,GO:0005783,GO:0005788,GO:0005794,GO:0006996,GO:0006997,GO:0006998,GO:0008150,GO:0009987,GO:0010256,GO:0012505,GO:0016020,GO:0016021,GO:0016043,GO:0019866,GO:0031090,GO:0031224,GO:0031229,GO:0031300,GO:0031301,GO:0031965,GO:0031967,GO:0031974,GO:0031975,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043621,GO:0044422,GO:0044424,GO:0044425,GO:0044428,GO:0044432,GO:0044444,GO:0044446,GO:0044453,GO:0044464,GO:0061024,GO:0070013,GO:0071763,GO:0071840
EggNOG free text desc.nuclear membrane organization
EggNOG OGs28KAC@1,2QSR2@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons1
Model size627
Cds size627
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816712.676097-CDS-M-pyrifera_M_contig80788:25..6521622816712.676097-CDS-M-pyrifera_M_contig80788:25..652Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig80788 26..652 -
1692278135.158817-CDS-M-pyrifera_M_contig80788:25..6521692278135.158817-CDS-M-pyrifera_M_contig80788:25..652Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig80788 26..652 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig80788.18901.1prot_M-pyrifera_M_contig80788.18901.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig80788 26..652 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig80788.18901.1

>prot_M-pyrifera_M_contig80788.18901.1 ID=prot_M-pyrifera_M_contig80788.18901.1|Name=mRNA_M-pyrifera_M_contig80788.18901.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=209bp
MPIRRGRTVRLTPGQAAANEACSRFLLKFIVGLFLIAVPLGLCWAEYEQR
EMHEAFEEAKQQAISVTPEAPVSQMNVGSLVHVSTRDVRTEETLVDEDFA
VQGPPNALKLQRKSQYCQWIEHVSEKKNRDGDVVRSYWYTKSWVPVPVPS
LFFDQPFRYNNPQRAPYANGALAVSEAQLGSYTIGPRLIDSLPVAEPVRL
ETFPSDALV
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mRNA from alignment at M-pyrifera_M_contig80788:26..652-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig80788.18901.1 ID=mRNA_M-pyrifera_M_contig80788.18901.1|Name=mRNA_M-pyrifera_M_contig80788.18901.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=627bp|location=Sequence derived from alignment at M-pyrifera_M_contig80788:26..652- (Macrocystis pyrifera P11B4 male)
ATGCCGATTAGACGAGGCCGGACTGTCAGGCTGACGCCTGGCCAAGCGGC AGCGAACGAAGCCTGCTCGAGGTTTCTCCTGAAGTTCATCGTGGGCCTCT TCCTCATTGCAGTGCCGCTGGGCCTCTGCTGGGCAGAGTATGAGCAACGG GAGATGCACGAGGCCTTCGAGGAGGCGAAGCAGCAGGCGATTAGCGTGAC ACCAGAGGCGCCAGTGAGCCAAATGAACGTTGGGAGTCTCGTGCACGTGA GCACACGTGACGTGCGGACAGAAGAGACGTTGGTGGACGAGGACTTCGCA GTGCAGGGGCCGCCCAATGCGCTGAAGCTGCAGAGGAAGAGCCAGTACTG CCAGTGGATCGAGCACGTGAGCGAGAAGAAGAACAGGGATGGAGACGTGG TGCGGAGCTACTGGTACACCAAGTCCTGGGTGCCCGTGCCGGTGCCCTCT CTCTTCTTCGACCAGCCCTTCCGCTACAACAATCCCCAGCGTGCGCCTTA TGCCAACGGCGCCCTGGCAGTCTCTGAGGCGCAGTTGGGCTCCTATACCA TTGGCCCCCGCCTCATTGACTCTCTCCCTGTAGCAGAGCCGGTGAGGCTC GAGACATTCCCCTCCGACGCCCTCGTC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig80788:26..652-

>mRNA_M-pyrifera_M_contig80788.18901.1 ID=mRNA_M-pyrifera_M_contig80788.18901.1|Name=mRNA_M-pyrifera_M_contig80788.18901.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1254bp|location=Sequence derived from alignment at M-pyrifera_M_contig80788:26..652- (Macrocystis pyrifera P11B4 male)
ATGCCGATTAGACGAGGCCGGACTGTCAGGCTGACGCCTGGCCAAGCGGC
AGCGAACGAAGCCTGCTCGAGGTTTCTCCTGAAGTTCATCGTGGGCCTCT
TCCTCATTGCAGTGCCGCTGGGCCTCTGCTGGGCAGAGTATGAGCAACGG
GAGATGCACGAGGCCTTCGAGGAGGCGAAGCAGCAGGCGATTAGCGTGAC
ACCAGAGGCGCCAGTGAGCCAAATGAACGTTGGGAGTCTCGTGCACGTGA
GCACACGTGACGTGCGGACAGAAGAGACGTTGGTGGACGAGGACTTCGCA
GTGCAGGGGCCGCCCAATGCGCTGAAGCTGCAGAGGAAGAGCCAGTACTG
CCAGTGGATCGAGCACGTGAGCGAGAAGAAGAACAGGGATGGAGACGTGG
TGCGGAGCTACTGGTACACCAAGTCCTGGGTGCCCGTGCCGGTGCCCTCT
CTCTTCTTCGACCAGCCCTTCCGCTACAACAATCCCCAGCGTGCGCCTTA
TGCCAACGGCGCCCTGGCAGTCTCTGAGGCGCAGTTGGGCTCCTATACCA
TTGGCCCCCGCCTCATTGACTCTCTCCCTGTAGCAGAGCCGGTGAGGCTC
GAGACATTCCCCTCCGACGCCCTCGTCATGCCGATTAGACGAGGCCGGAC
TGTCAGGCTGACGCCTGGCCAAGCGGCAGCGAACGAAGCCTGCTCGAGGT
TTCTCCTGAAGTTCATCGTGGGCCTCTTCCTCATTGCAGTGCCGCTGGGC
CTCTGCTGGGCAGAGTATGAGCAACGGGAGATGCACGAGGCCTTCGAGGA
GGCGAAGCAGCAGGCGATTAGCGTGACACCAGAGGCGCCAGTGAGCCAAA
TGAACGTTGGGAGTCTCGTGCACGTGAGCACACGTGACGTGCGGACAGAA
GAGACGTTGGTGGACGAGGACTTCGCAGTGCAGGGGCCGCCCAATGCGCT
GAAGCTGCAGAGGAAGAGCCAGTACTGCCAGTGGATCGAGCACGTGAGCG
AGAAGAAGAACAGGGATGGAGACGTGGTGCGGAGCTACTGGTACACCAAG
TCCTGGGTGCCCGTGCCGGTGCCCTCTCTCTTCTTCGACCAGCCCTTCCG
CTACAACAATCCCCAGCGTGCGCCTTATGCCAACGGCGCCCTGGCAGTCT
CTGAGGCGCAGTTGGGCTCCTATACCATTGGCCCCCGCCTCATTGACTCT
CTCCCTGTAGCAGAGCCGGTGAGGCTCGAGACATTCCCCTCCGACGCCCT
CGTC
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