mRNA_M-pyrifera_M_contig80752.18897.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig80752.18897.1
Unique NamemRNA_M-pyrifera_M_contig80752.18897.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig80752.18897.1 vs. uniprot
Match: A0A058ZYG8_EUCGR (N6-L-threonylcarbamoyladenine synthase n=1 Tax=Eucalyptus grandis TaxID=71139 RepID=A0A058ZYG8_EUCGR)

HSP 1 Score: 159 bits (402), Expect = 9.590e-48
Identity = 81/126 (64.29%), Postives = 95/126 (75.40%), Query Frame = 1 
Query:    1 GEAFVADMCFSLQETVFAMLVEVTERAMAHSGAKDVLIVGGVGCNKRLQEMAGVMARERGGAVAGMDDRYCIDNGAMIAHTGLLMFQHNLSTPIHASKCSQRFRTDQVEVVWREKYREEETRRGGG 378
             E   AD+C+SLQET+FAMLVE+TERAMAH   KDVLIVGGVGCN+RLQEM  VM  ERGG +   DDRYCIDNGAMIA+TGLL + H  STP+  S  +QRFRTD+V  +WREK   EE ++  G
Sbjct:   23 NECTPADLCYSLQETLFAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRVMCSERGGKLYATDDRYCIDNGAMIAYTGLLAYAHGTSTPLEESTFTQRFRTDEVHAIWREK---EELKKANG 145
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BLAST of mRNA_M-pyrifera_M_contig80752.18897.1 vs. uniprot
Match: A0A1Y5IG66_OSTTA (Probable tRNA N6-adenosine threonylcarbamoyltransferase n=2 Tax=Ostreococcus tauri TaxID=70448 RepID=A0A1Y5IG66_OSTTA)

HSP 1 Score: 164 bits (416), Expect = 3.010e-47
Identity = 83/130 (63.85%), Postives = 101/130 (77.69%), Query Frame = 1 
Query:    1 GEAFVADMCFSLQETVFAMLVEVTERAMAHSGAKDVLIVGGVGCNKRLQEMAGVMARERGGAVAG------MDDRYCIDNGAMIAHTGLLMFQHNLSTPIHASKCSQRFRTDQVEVVWREKYREEETRRG 372
            GE  VAD+CFS+QETVF+MLVE+TERAMAH  AKDV+IVGGVGCN RLQEM  +MARERGG + G      MDDR+CIDNGAMIAHTGL+ + +  +TPI  ++C+QRFRTD+V V WR+K   + T  G
Sbjct:  231 GEVTVADLCFSMQETVFSMLVEITERAMAHVNAKDVMIVGGVGCNLRLQEMMAIMARERGGKLYGLDENGRMDDRFCIDNGAMIAHTGLIQYLNGETTPIEETECTQRFRTDEVLVTWRDKAGTKRTAEG 360
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BLAST of mRNA_M-pyrifera_M_contig80752.18897.1 vs. uniprot
Match: A0A804LFF7_MAIZE (N6-L-threonylcarbamoyladenine synthase n=6 Tax=Zea mays TaxID=4577 RepID=A0A804LFF7_MAIZE)

HSP 1 Score: 159 bits (402), Expect = 5.000e-47
Identity = 76/115 (66.09%), Postives = 91/115 (79.13%), Query Frame = 1 
Query:    1 GEAFVADMCFSLQETVFAMLVEVTERAMAHSGAKDVLIVGGVGCNKRLQEMAGVMARERGGAVAGMDDRYCIDNGAMIAHTGLLMFQHNLSTPIHASKCSQRFRTDQVEVVWREK 345
             E   AD+C+SLQET+FAMLVE+TE AMAH  +KDVLIVGGVGCNKRLQEM  +M  ERGG +   DDRYCIDNGAMIA+TGLL + H ++TP+  S  +QRFRTD+V  +WREK
Sbjct:   57 NECTPADLCYSLQETIFAMLVEITESAMAHCDSKDVLIVGGVGCNKRLQEMMKIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAYAHGMTTPLEESTFTQRFRTDEVHAIWREK 171
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BLAST of mRNA_M-pyrifera_M_contig80752.18897.1 vs. uniprot
Match: A0A8K1CIJ7_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CIJ7_PYTOL)

HSP 1 Score: 163 bits (413), Expect = 6.680e-47
Identity = 77/114 (67.54%), Postives = 93/114 (81.58%), Query Frame = 1 
Query:    1 GEAFVADMCFSLQETVFAMLVEVTERAMAHSGAKDVLIVGGVGCNKRLQEMAGVMARERGGAVAGMDDRYCIDNGAMIAHTGLLMFQHNLSTPIHASKCSQRFRTDQVEVVWRE 342
            GE    D+C+SLQET+FAMLVE+TERAMAH G  +VLIVGGVGCNKRLQEM  +MA+ERGG V  MD RYCIDNGAMIA  G+L +Q++  TP+H + C+QRFRTD+V V+WRE
Sbjct:  241 GEYTKEDLCYSLQETIFAMLVEITERAMAHCGQSEVLIVGGVGCNKRLQEMMEIMAKERGGRVCAMDHRYCIDNGAMIAQAGVLQYQYSDITPLHEATCTQRFRTDEVHVIWRE 354
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BLAST of mRNA_M-pyrifera_M_contig80752.18897.1 vs. uniprot
Match: A0A3L6D7P0_MAIZE (N6-L-threonylcarbamoyladenine synthase n=1 Tax=Zea mays TaxID=4577 RepID=A0A3L6D7P0_MAIZE)

HSP 1 Score: 160 bits (404), Expect = 1.180e-46
Identity = 76/115 (66.09%), Postives = 92/115 (80.00%), Query Frame = 1 
Query:    1 GEAFVADMCFSLQETVFAMLVEVTERAMAHSGAKDVLIVGGVGCNKRLQEMAGVMARERGGAVAGMDDRYCIDNGAMIAHTGLLMFQHNLSTPIHASKCSQRFRTDQVEVVWREK 345
             E   AD+C+SLQET+FAMLVE+TERAMAH  +KDVLIVGGVGCN+RLQEM  +M  ERGG +   DDRYCIDNGAMIA+TGLL + H ++TP+  S  +QRFRTD+V  +WREK
Sbjct:  138 NECTPADLCYSLQETIFAMLVEITERAMAHCDSKDVLIVGGVGCNERLQEMMKIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAYAHGMTTPLEESTFTQRFRTDEVHAIWREK 252
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BLAST of mRNA_M-pyrifera_M_contig80752.18897.1 vs. uniprot
Match: D7TZ32_VITVI (N6-L-threonylcarbamoyladenine synthase n=4 Tax=Vitis TaxID=3603 RepID=D7TZ32_VITVI)

HSP 1 Score: 156 bits (395), Expect = 1.220e-46
Identity = 77/115 (66.96%), Postives = 89/115 (77.39%), Query Frame = 1 
Query:    1 GEAFVADMCFSLQETVFAMLVEVTERAMAHSGAKDVLIVGGVGCNKRLQEMAGVMARERGGAVAGMDDRYCIDNGAMIAHTGLLMFQHNLSTPIHASKCSQRFRTDQVEVVWREK 345
             E   AD+C+SLQETVFAMLVE+TERAMAH   KDVLIVGGVGCN+RLQEM  VM  ER G +   DDRYCIDNGAMIA+TGLL + H  +TP+  S  +QRFRTD+V  +WREK
Sbjct:   23 NECTPADLCYSLQETVFAMLVEITERAMAHCDKKDVLIVGGVGCNERLQEMMRVMCSERSGRLFATDDRYCIDNGAMIAYTGLLAYAHGATTPLEESTFTQRFRTDEVHAIWREK 137
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BLAST of mRNA_M-pyrifera_M_contig80752.18897.1 vs. uniprot
Match: I3SQ27_LOTJA (N6-L-threonylcarbamoyladenine synthase n=1 Tax=Lotus japonicus TaxID=34305 RepID=I3SQ27_LOTJA)

HSP 1 Score: 156 bits (394), Expect = 1.270e-46
Identity = 76/114 (66.67%), Postives = 90/114 (78.95%), Query Frame = 1 
Query:    1 GEAFVADMCFSLQETVFAMLVEVTERAMAHSGAKDVLIVGGVGCNKRLQEMAGVMARERGGAVAGMDDRYCIDNGAMIAHTGLLMFQHNLSTPIHASKCSQRFRTDQVEVVWRE 342
             E   AD+C+SLQET+FAMLVE+TERAMAH  +KDVLIVGGVGCN+RLQEM   M  ERGG +   DDRYCIDNGAMIA+TGLL + H  STP+  S  +QRFRTD+V+ +WRE
Sbjct:   23 NECTPADLCYSLQETLFAMLVEITERAMAHCDSKDVLIVGGVGCNERLQEMMRTMCSERGGRLFATDDRYCIDNGAMIAYTGLLEYAHGASTPLEDSTFTQRFRTDEVKAIWRE 136
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BLAST of mRNA_M-pyrifera_M_contig80752.18897.1 vs. uniprot
Match: A0A1U8MPA9_GOSHI (Probable tRNA N6-adenosine threonylcarbamoyltransferase n=9 Tax=Pentapetalae TaxID=1437201 RepID=A0A1U8MPA9_GOSHI)

HSP 1 Score: 162 bits (411), Expect = 1.300e-46
Identity = 79/115 (68.70%), Postives = 91/115 (79.13%), Query Frame = 1 
Query:    1 GEAFVADMCFSLQETVFAMLVEVTERAMAHSGAKDVLIVGGVGCNKRLQEMAGVMARERGGAVAGMDDRYCIDNGAMIAHTGLLMFQHNLSTPIHASKCSQRFRTDQVEVVWREK 345
             E   AD+C+SLQET+FAMLVE+TERAMAH  +KDVLIVGGVGCN+RLQEM  +M  ERGG +   DDRYCIDNGAMIAHTGLL F H  STP+  S  +QRFRTD+V  +WREK
Sbjct:  226 NECTPADLCYSLQETLFAMLVEITERAMAHCDSKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAHTGLLAFAHGYSTPLEESTFTQRFRTDEVHAIWREK 340
[Close]
BLAST of mRNA_M-pyrifera_M_contig80752.18897.1 vs. uniprot
Match: A0A452Y5X4_AEGTS (N6-L-threonylcarbamoyladenine synthase n=2 Tax=Triticinae TaxID=1648030 RepID=A0A452Y5X4_AEGTS)

HSP 1 Score: 158 bits (400), Expect = 1.310e-46
Identity = 76/115 (66.09%), Postives = 92/115 (80.00%), Query Frame = 1 
Query:    1 GEAFVADMCFSLQETVFAMLVEVTERAMAHSGAKDVLIVGGVGCNKRLQEMAGVMARERGGAVAGMDDRYCIDNGAMIAHTGLLMFQHNLSTPIHASKCSQRFRTDQVEVVWREK 345
             E   AD+C+SLQET+FAMLVE+TERAMAH  +KDVLIVGGVGCN+RLQEM  +M  ERGG +   DDRYCIDNGAMIA+TGLL + H ++TP+  S  +QRFRTD+V  +WREK
Sbjct:   66 NECTPADLCYSLQETLFAMLVEITERAMAHCDSKDVLIVGGVGCNERLQEMMRIMCSERGGRLFATDDRYCIDNGAMIAYTGLLAYAHGVTTPLEDSTFTQRFRTDEVHAIWREK 180
[Close]
BLAST of mRNA_M-pyrifera_M_contig80752.18897.1 vs. uniprot
Match: A0A0C2Z4I1_HEBCY (N(6)-L-threonylcarbamoyladenine synthase n=1 Tax=Hebeloma cylindrosporum h7 TaxID=686832 RepID=A0A0C2Z4I1_HEBCY)

HSP 1 Score: 160 bits (405), Expect = 1.560e-46
Identity = 79/108 (73.15%), Postives = 91/108 (84.26%), Query Frame = 1 
Query:   16 ADMCFSLQETVFAMLVEVTERAMAHSGAKDVLIVGGVGCNKRLQEMAGVMARERGGAVAGMDDRYCIDNGAMIAHTGLLMFQHNLSTPIHASKCSQRFRTDQVEVVWR 339
            AD+CFSLQETVFAMLVE+TERAMAH G+K+VLIVGGVGCN+RLQEM GVMARERGG +   D+R+CIDNG MIA  GLL F+  L+TP+  S C+QRFRTDQV V WR
Sbjct:  171 ADLCFSLQETVFAMLVEITERAMAHIGSKEVLIVGGVGCNERLQEMMGVMARERGGQLFATDERFCIDNGIMIAQAGLLSFRMGLTTPLGKSTCTQRFRTDQVFVSWR 278
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig80752.18897.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A058ZYG8_EUCGR9.590e-4864.29N6-L-threonylcarbamoyladenine synthase n=1 Tax=Euc... [more]
A0A1Y5IG66_OSTTA3.010e-4763.85Probable tRNA N6-adenosine threonylcarbamoyltransf... [more]
A0A804LFF7_MAIZE5.000e-4766.09N6-L-threonylcarbamoyladenine synthase n=6 Tax=Zea... [more]
A0A8K1CIJ7_PYTOL6.680e-4767.54Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A3L6D7P0_MAIZE1.180e-4666.09N6-L-threonylcarbamoyladenine synthase n=1 Tax=Zea... [more]
D7TZ32_VITVI1.220e-4666.96N6-L-threonylcarbamoyladenine synthase n=4 Tax=Vit... [more]
I3SQ27_LOTJA1.270e-4666.67N6-L-threonylcarbamoyladenine synthase n=1 Tax=Lot... [more]
A0A1U8MPA9_GOSHI1.300e-4668.70Probable tRNA N6-adenosine threonylcarbamoyltransf... [more]
A0A452Y5X4_AEGTS1.310e-4666.09N6-L-threonylcarbamoyladenine synthase n=2 Tax=Tri... [more]
A0A0C2Z4I1_HEBCY1.560e-4673.15N(6)-L-threonylcarbamoyladenine synthase n=1 Tax=H... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig80752contigM-pyrifera_M_contig80752:44..427 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score158.7
Seed ortholog evalue1.7e-36
Seed eggNOG ortholog161934.XP_010672249.1
Preferred nameGCP2
KEGG rclassRC00070,RC00416
KEGG koko:K01409
KEGG ReactionR10648
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Component of the EKC KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. Likely plays a direct catalytic role in this reaction, but requires other protein(s) of the complex to fulfill this activity
EggNOG OGs37P73@33090,3GBBG@35493,COG0533@1,KOG2708@2759
EC2.3.1.234
COG Functional cat.O
Best tax levelStreptophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03016
Exons1
Model size384
Cds size363
Stop1
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816712.4336722-UTR-M-pyrifera_M_contig80752:43..641622816712.4336722-UTR-M-pyrifera_M_contig80752:43..64Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig80752 44..64 +
1692278135.0038548-UTR-M-pyrifera_M_contig80752:43..641692278135.0038548-UTR-M-pyrifera_M_contig80752:43..64Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig80752 44..64 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816712.4452276-CDS-M-pyrifera_M_contig80752:64..4271622816712.4452276-CDS-M-pyrifera_M_contig80752:64..427Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig80752 65..427 +
1692278135.017334-CDS-M-pyrifera_M_contig80752:64..4271692278135.017334-CDS-M-pyrifera_M_contig80752:64..427Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig80752 65..427 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig80752.18897.1prot_M-pyrifera_M_contig80752.18897.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig80752 65..427 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig80752.18897.1

>prot_M-pyrifera_M_contig80752.18897.1 ID=prot_M-pyrifera_M_contig80752.18897.1|Name=mRNA_M-pyrifera_M_contig80752.18897.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=121bp
MCFSLQETVFAMLVEVTERAMAHSGAKDVLIVGGVGCNKRLQEMAGVMAR
ERGGAVAGMDDRYCIDNGAMIAHTGLLMFQHNLSTPIHASKCSQRFRTDQ
VEVVWREKYREEETRRGGGE*
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mRNA from alignment at M-pyrifera_M_contig80752:44..427+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig80752.18897.1 ID=mRNA_M-pyrifera_M_contig80752.18897.1|Name=mRNA_M-pyrifera_M_contig80752.18897.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=384bp|location=Sequence derived from alignment at M-pyrifera_M_contig80752:44..427+ (Macrocystis pyrifera P11B4 male)
GGGGAGGCGTTTGTGGCAGATATGTGCTTTTCTCTGCAAGAAACGGTGTT
TGCCATGTTGGTGGAGGTCACAGAGCGTGCCATGGCACACAGTGGTGCCA
AAGACGTGTTGATCGTGGGTGGAGTGGGATGCAACAAGAGATTGCAAGAA
ATGGCCGGGGTCATGGCTCGAGAACGTGGTGGAGCTGTGGCAGGCATGGA
TGACAGGTATTGCATCGACAATGGCGCCATGATTGCACACACCGGACTTC
TCATGTTCCAGCACAACCTTTCCACCCCAATCCACGCCTCAAAGTGCTCC
CAAAGGTTCAGAACTGACCAAGTTGAGGTGGTGTGGAGGGAAAAGTACAG
GGAAGAAGAGACCAGAAGGGGTGGAGGGGAGTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig80752:44..427+

>mRNA_M-pyrifera_M_contig80752.18897.1 ID=mRNA_M-pyrifera_M_contig80752.18897.1|Name=mRNA_M-pyrifera_M_contig80752.18897.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=726bp|location=Sequence derived from alignment at M-pyrifera_M_contig80752:44..427+ (Macrocystis pyrifera P11B4 male)
ATGTGCTTTTCTCTGCAAGAAACGGTGTTTGCCATGTTGGTGGAGGTCAC
AGAGCGTGCCATGGCACACAGTGGTGCCAAAGACGTGTTGATCGTGGGTG
GAGTGGGATGCAACAAGAGATTGCAAGAAATGGCCGGGGTCATGGCTCGA
GAACGTGGTGGAGCTGTGGCAGGCATGGATGACAGGTATTGCATCGACAA
TGGCGCCATGATTGCACACACCGGACTTCTCATGTTCCAGCACAACCTTT
CCACCCCAATCCACGCCTCAAAGTGCTCCCAAAGGTTCAGAACTGACCAA
GTTGAGGTGGTGTGGAGGGAAAAGTACAGGGAAGAAGAGACCAGAAGGGG
TGGAGGGGAGTGAATGTGCTTTTCTCTGCAAGAAACGGTGTTTGCCATGT
TGGTGGAGGTCACAGAGCGTGCCATGGCACACAGTGGTGCCAAAGACGTG
TTGATCGTGGGTGGAGTGGGATGCAACAAGAGATTGCAAGAAATGGCCGG
GGTCATGGCTCGAGAACGTGGTGGAGCTGTGGCAGGCATGGATGACAGGT
ATTGCATCGACAATGGCGCCATGATTGCACACACCGGACTTCTCATGTTC
CAGCACAACCTTTCCACCCCAATCCACGCCTCAAAGTGCTCCCAAAGGTT
CAGAACTGACCAAGTTGAGGTGGTGTGGAGGGAAAAGTACAGGGAAGAAG
AGACCAGAAGGGGTGGAGGGGAGTGA
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