mRNA_M-pyrifera_M_contig80566.18868.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig80566.18868.1
Unique NamemRNA_M-pyrifera_M_contig80566.18868.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig80566.18868.1 vs. uniprot
Match: A0A5A8DMM8_CAFRO (J domain-containing protein n=3 Tax=Sar TaxID=2698737 RepID=A0A5A8DMM8_CAFRO)

HSP 1 Score: 199 bits (507), Expect = 1.790e-60
Identity = 101/168 (60.12%), Postives = 131/168 (77.98%), Query Frame = 1
Query:    1 SLEELCLGCTKRIRVTRKRASESGD-LRDDSKILEIRVKPGWKAGTKITFEGEGDEARGVLPGDIVFVVAEKAHDKFKRDGDDLILNQRVSLLQALTECTVEVPTLDGRTIPVACNEVISPNYTKRISGEGMVNSKS-GRKGDIVIKFQIDFPAHLSEERRALVRRAL 498
            SLEEL  G TKR++VTR+R    G  LR + K+LEI VKPGWKAGT+ITFE EGD+A GV+P DIVFV+AEK H +F R+G+DL++  RV+LLQALT  T  V TLD RT+ VACNEV+SP Y KR+ GEGM  SK+ G +GD+V++F++ FP+H+ E++R L+RRAL
Sbjct:  173 SLEELFNGATKRMKVTRQRLQADGSTLRPEEKLLEIHVKPGWKAGTRITFENEGDQAPGVVPADIVFVIAEKHHPRFTREGNDLVVKARVTLLQALTTHTQSVRTLDDRTLSVACNEVLSPGYEKRVRGEGMPISKAPGTRGDLVLRFEVVFPSHIPEDKRDLLRRAL 340          
BLAST of mRNA_M-pyrifera_M_contig80566.18868.1 vs. uniprot
Match: A0A7S1CED9_9STRA (Hypothetical protein n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CED9_9STRA)

HSP 1 Score: 193 bits (490), Expect = 3.340e-58
Identity = 99/170 (58.24%), Postives = 125/170 (73.53%), Query Frame = 1
Query:    1 SLEELCLGCTKRIRVTRKRASESG-DLRDDSKILEIRVKPGWKAGTKITFEGEGDEARGVLPGDIVFVVAEKAHDKFKRDGDDLILNQRVSLLQALTECTVEVPTLDGRTIPVACNEVISPNYTKRISGEGMVNSKS---GRKGDIVIKFQIDFPAHLSEERRALVRRAL 498
            SLEEL  GCTKRIR+TRK+ +  G   R + KILEIRVK GWKAGT ITF  EGDE  G++P DI FVVAEK H +F RDG++L+ N RV+L  ALT+C VEV TLDGRT+ + CNEVISP  TK + GEGM  SK+   G +GD+++KF I FP+H+SE+++  +R  L
Sbjct:  149 SLEELFNGCTKRIRITRKKLNPDGRTTRMEEKILEIRVKAGWKAGTTITFPNEGDEGPGIVPADIAFVVAEKPHPRFSRDGNNLVHNARVTLRDALTDCVVEVATLDGRTLSIPCNEVISPGATKVVPGEGMPISKAAAAGTRGDLILKFTIVFPSHISEDKKRQLRTLL 318          
BLAST of mRNA_M-pyrifera_M_contig80566.18868.1 vs. uniprot
Match: A0A7S3UUU7_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3UUU7_HETAK)

HSP 1 Score: 187 bits (476), Expect = 1.420e-55
Identity = 88/168 (52.38%), Postives = 123/168 (73.21%), Query Frame = 1
Query:    1 SLEELCLGCTKRIRVTRKRASESGDLRDDSKILEIRVKPGWKAGTKITFEGEGDEARGVLPGDIVFVVAEKAHDKFKRDGDDLILNQRVSLLQALTECTVEVPTLDGRTIPVACNEVISPNYTKRISGEGMVNSKS-GRKGDIVIKFQIDFPAHLSEERRALVRRALG 501
            SLEEL  GCTKR+++TRKR +  GDL D+ KI+ I VKPGWK GTKITF  EGDE   V+P D+VFV+ E+ HD F R+G++L+ N +++L  ALT+C +EVPTLDGR + + C EV++P Y KR+ GEGM  SK  GR+G ++++F++ FP +L E ++  +RR L 
Sbjct:  163 SLEELFNGCTKRLKITRKRFTPEGDLVDEDKIVTINVKPGWKKGTKITFPCEGDEKPNVIPADVVFVITEQEHDYFGREGNNLVYNSKITLADALTDCCIEVPTLDGRVLSLPCPEVVAPGYEKRLEGEGMPLSKQPGRRGALLVRFRLLFPKYLPEHKKVELRRLLA 330          
BLAST of mRNA_M-pyrifera_M_contig80566.18868.1 vs. uniprot
Match: A0A7S2V156_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2V156_9STRA)

HSP 1 Score: 186 bits (472), Expect = 2.020e-55
Identity = 89/168 (52.98%), Postives = 125/168 (74.40%), Query Frame = 1
Query:    1 SLEELCLGCTKRIRVTRKRASESGDLRDDSKILEIRVKPGWKAGTKITFEGEGDEARGVLPGDIVFVVAEKAHDKFKRDGDDLILNQRVSLLQALTECTVEVPTLDGRTIPVACNEVISPNYTKRISGEGM-VNSKSGRKGDIVIKFQIDFPAHLSEERRALVRRALG 501
            +LEEL  GCTKR+++TR R   SG+L + +K+L I VKPGWK GTKITF  EGDE   ++P D+VFVV EK H+ F R+G +LI N +++L  ALT+C +EVPTLDGRT+ + C EV+SP Y K I GEGM ++ K G++G+++I+F+I FP +L EE++A +R+ L 
Sbjct:  144 TLEELFNGCTKRLKITRTRFDTSGELVESTKMLTINVKPGWKKGTKITFPCEGDERPNIIPADVVFVVTEKEHEFFGREGSNLIYNAQITLADALTDCCIEVPTLDGRTLSLPCPEVVSPGYEKTIVGEGMPLSKKVGQRGELIIRFKILFPRYLPEEKKAKLRQLLA 311          
BLAST of mRNA_M-pyrifera_M_contig80566.18868.1 vs. uniprot
Match: D8LSP2_ECTSI (Flagellar radial spoke protein 16 n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LSP2_ECTSI)

HSP 1 Score: 187 bits (475), Expect = 2.990e-55
Identity = 92/168 (54.76%), Postives = 121/168 (72.02%), Query Frame = 1
Query:    1 SLEELCLGCTKRIRVTRKRASESGDLRDDSKILEIRVKPGWKAGTKITFEGEGDEARGVLPGDIVFVVAEKAHDKFKRDGDDLILNQRVSLLQALTECTVEVPTLDGRTIPVACNEVISPNYTKRISGEGMVNSKS-GRKGDIVIKFQIDFPAHLSEERRALVRRALG 501
            SLEEL  GCTK  +VTRKR +E+G+L + S  L + VKPGWK GTKITF GEGDE  GVLP D+V VVAE+ H+ F R+G+DLI    +SL  ALT+C +EVPTLDGR + + C EV+SP Y +R+ GEGM  SK+ G +GD++I+F++ FPA L    + ++RR L 
Sbjct:  147 SLEELYNGCTKAFKVTRKRLNEAGELAEASTQLTVAVKPGWKKGTKITFPGEGDEGAGVLPADVVLVVAERPHEYFSREGNDLIYTSMLSLADALTDCIIEVPTLDGRVLRLPCPEVVSPGYERRLEGEGMPISKNPGSRGDLLIRFKLVFPAFLPHASKVVLRRLLS 314          
BLAST of mRNA_M-pyrifera_M_contig80566.18868.1 vs. uniprot
Match: S8E0N3_9LAMI (J domain-containing protein n=1 Tax=Genlisea aurea TaxID=192259 RepID=S8E0N3_9LAMI)

HSP 1 Score: 178 bits (452), Expect = 3.180e-53
Identity = 91/166 (54.82%), Postives = 113/166 (68.07%), Query Frame = 1
Query:    1 SLEELCLGCTKRIRVTRKRASESGDLRDDSKILEIRVKPGWKAGTKITFEGEGDEARGVLPGDIVFVVAEKAHDKFKRDGDDLILNQRVSLLQALTECTVEVPTLDGRTIPVACNEVISPNYTKRISGEGMVNSKSGRKGDIVIKFQIDFPAHLSEERRALVRRAL 498
            SLEELC GC K+I++TR   S SG L  + +IL I+VKPGWK GTKITFEG+GDE  G LP DI+F + EK H  FKR+GDDL L   V L+QALT CTV VP L G  I ++ +++I P Y K I GEGM   K GR+GD+ +KF +DF   L +E+R  V R L
Sbjct:   88 SLEELCNGCIKKIKITRDSISSSGLLVQEDEILMIKVKPGWKRGTKITFEGKGDERPGALPADIIFSIEEKQHPLFKREGDDLELGVEVPLVQALTGCTVPVPLLGGGEITLSIDDIIYPGYEKTIPGEGMPKPKEGRRGDLRLKFLVDFQTELRDEQRQEVVRIL 253          
BLAST of mRNA_M-pyrifera_M_contig80566.18868.1 vs. uniprot
Match: A0A836C9Z0_9STRA (DnaJ domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C9Z0_9STRA)

HSP 1 Score: 177 bits (449), Expect = 9.460e-52
Identity = 83/169 (49.11%), Postives = 122/169 (72.19%), Query Frame = 1
Query:    1 SLEELCLGCTKRIRVTRKRASESGDLRDDSKILEIRVKPGWKAGTKITFEGEGDEARGVLPGDIVFVVAEKAHDKFKR-DGDDLILNQRVSLLQALTECTVEVPTLDGRTIPVACNEVISPNYTKRISGEGM-VNSKSGRKGDIVIKFQIDFPAHLSEERRALVRRALG 501
            +LEEL  GCTKR+++TRKR +  G+LRD+SK + + V+PGWK GT++TF  EGDEA G++P D+VFVV E  H +F R DG  L+    +SL  ALT+C +EVPTLDGR + + C EV+SP Y KR+  EGM ++ K G++GD++++F+I FP +L ++++  +R  L 
Sbjct:  143 TLEELFNGCTKRLKITRKRFAPDGELRDESKEVTVDVRPGWKRGTRVTFPSEGDEAPGIVPADVVFVVQEAPHARFSRGDGAQLVYCAPLSLADALTDCVLEVPTLDGRVLSLPCPEVVSPGYEKRVPKEGMPISKKPGQRGDLILRFKIVFPKYLPDDKKLKIRHILA 311          
BLAST of mRNA_M-pyrifera_M_contig80566.18868.1 vs. uniprot
Match: A0A2G9HLL7_9LAMI (Molecular chaperone (DnaJ superfamily) n=1 Tax=Handroanthus impetiginosus TaxID=429701 RepID=A0A2G9HLL7_9LAMI)

HSP 1 Score: 177 bits (450), Expect = 1.450e-51
Identity = 85/162 (52.47%), Postives = 115/162 (70.99%), Query Frame = 1
Query:    1 SLEELCLGCTKRIRVTRKRASESGDLRDDSKILEIRVKPGWKAGTKITFEGEGDEARGVLPGDIVFVVAEKAHDKFKRDGDDLILNQRVSLLQALTECTVEVPTLDGRTIPVACNEVISPNYTKRISGEGMVNSKSGRKGDIVIKFQIDFPAHLSEERRALV 486
            +LEELC GC K+I++TR   S SG +  + +IL I+VKPGWK GTKITFEG+GDE  G LP DI+F + EK H  FKR+GDDL L   V L+QALT CT+ VP L G  I ++ +++I P Y K I G+GM  SK G++GD+ +KF ++FP  LS+++R+ V
Sbjct:  205 TLEELCHGCVKKIKITRDAISSSGLIVQEEEILMIKVKPGWKKGTKITFEGKGDERPGTLPADIIFSIDEKRHPMFKREGDDLALGVEVPLVQALTGCTIPVPLLGGGQITLSIDDIIYPGYEKIIPGQGMPTSKDGKRGDLKLKFLVEFPTELSDDQRSEV 366          
BLAST of mRNA_M-pyrifera_M_contig80566.18868.1 vs. uniprot
Match: A0A813A4M3_9DINO (DNAJB4 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813A4M3_9DINO)

HSP 1 Score: 176 bits (446), Expect = 1.860e-51
Identity = 91/161 (56.52%), Postives = 114/161 (70.81%), Query Frame = 1
Query:    1 SLEELCLGCTKRIRVTRKRASESGDLRDDSKILEIRVKPGWKAGTKITFEGEGDEARGVLPGDIVFVVAEKAHDKFKRDGDDLILNQRVSLLQALTECTVEVPTLDGRTIPVACNEVISPNYTKRISGEGMVNSKS-GRKGDIVIKFQIDFPAHLSEERRA 480
            SLE+L  G TK ++VTR+ A  S       K+L++ +K GWK GTKITFE EGDEA G +P DIVFV+ EK HD+F+RDG+DL+   R++L QAL + TV V TLDGRT+ V CNEVISP YTKR+ GEGM  SK+ G KGD+ +KF I FP H+    RA
Sbjct:  167 SLEDLYTGTTKHLKVTRRLADGS----TAEKVLDVPIKAGWKRGTKITFEKEGDEAPGTIPADIVFVIGEKPHDRFERDGNDLVHTARITLKQALCDHTVHVRTLDGRTLSVPCNEVISPGYTKRVPGEGMPVSKAPGSKGDLRLKFHITFPTHIDTATRA 323          
BLAST of mRNA_M-pyrifera_M_contig80566.18868.1 vs. uniprot
Match: A0A830CP17_9LAMI (Dnaj protein homolog 1 n=1 Tax=Phtheirospermum japonicum TaxID=374723 RepID=A0A830CP17_9LAMI)

HSP 1 Score: 175 bits (443), Expect = 1.200e-50
Identity = 87/166 (52.41%), Postives = 117/166 (70.48%), Query Frame = 1
Query:    1 SLEELCLGCTKRIRVTRKRASESGDLRDDSKILEIRVKPGWKAGTKITFEGEGDEARGVLPGDIVFVVAEKAHDKFKRDGDDLILNQRVSLLQALTECTVEVPTLDGRTIPVACNEVISPNYTKRISGEGMVNSKSGRKGDIVIKFQIDFPAHLSEERRALVRRAL 498
            +LEELC G  K+I++TR   S SG + ++ +IL I+VKPGWK GTKITFEG+GDE  G LP DI+F + EK H  FKR+GDDL L   V L+QALT CTV VP L G  I ++ +++I P Y K I G+GM  SK G++GD+ +KF ++FPA LS ++R+ + R L
Sbjct:  193 TLEELCHGGVKKIKITRDAISSSGLIVEEEEILMIKVKPGWKKGTKITFEGKGDERPGTLPADIIFCIDEKRHSLFKREGDDLELGVEVPLVQALTGCTVPVPLLGGGQINLSIDDIIYPGYEKIIPGQGMTISKDGKRGDLRLKFLVEFPAELSHDQRSEIVRIL 358          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig80566.18868.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8DMM8_CAFRO1.790e-6060.12J domain-containing protein n=3 Tax=Sar TaxID=2698... [more]
A0A7S1CED9_9STRA3.340e-5858.24Hypothetical protein n=1 Tax=Bicosoecida sp. CB-20... [more]
A0A7S3UUU7_HETAK1.420e-5552.38Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A7S2V156_9STRA2.020e-5552.98Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
D8LSP2_ECTSI2.990e-5554.76Flagellar radial spoke protein 16 n=1 Tax=Ectocarp... [more]
S8E0N3_9LAMI3.180e-5354.82J domain-containing protein n=1 Tax=Genlisea aurea... [more]
A0A836C9Z0_9STRA9.460e-5249.11DnaJ domain-containing protein n=1 Tax=Tribonema m... [more]
A0A2G9HLL7_9LAMI1.450e-5152.47Molecular chaperone (DnaJ superfamily) n=1 Tax=Han... [more]
A0A813A4M3_9DINO1.860e-5156.52DNAJB4 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=... [more]
A0A830CP17_9LAMI1.200e-5052.41Dnaj protein homolog 1 n=1 Tax=Phtheirospermum jap... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig80566contigM-pyrifera_M_contig80566:3..506 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score190.3
Seed ortholog evalue7.4e-46
Seed eggNOG ortholog2880.D8LSP2
KEGG koko:K09510,ko:K09519
Hectar predicted targeting categoryother localisation
EggNOG free text desc.protein folding
EggNOG OGsCOG0484@1,KOG0714@2759
COG Functional cat.O
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03110
Exons1
Model size504
Cds size504
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816711.1821332-CDS-M-pyrifera_M_contig80566:2..5061622816711.1821332-CDS-M-pyrifera_M_contig80566:2..506Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig80566 3..506 +
1692278134.4032927-CDS-M-pyrifera_M_contig80566:2..5061692278134.4032927-CDS-M-pyrifera_M_contig80566:2..506Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig80566 3..506 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig80566.18868.1prot_M-pyrifera_M_contig80566.18868.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig80566 3..506 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig80566.18868.1

>prot_M-pyrifera_M_contig80566.18868.1 ID=prot_M-pyrifera_M_contig80566.18868.1|Name=mRNA_M-pyrifera_M_contig80566.18868.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=168bp
SLEELCLGCTKRIRVTRKRASESGDLRDDSKILEIRVKPGWKAGTKITFE
GEGDEARGVLPGDIVFVVAEKAHDKFKRDGDDLILNQRVSLLQALTECTV
EVPTLDGRTIPVACNEVISPNYTKRISGEGMVNSKSGRKGDIVIKFQIDF
PAHLSEERRALVRRALG*
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mRNA from alignment at M-pyrifera_M_contig80566:3..506+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig80566.18868.1 ID=mRNA_M-pyrifera_M_contig80566.18868.1|Name=mRNA_M-pyrifera_M_contig80566.18868.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=504bp|location=Sequence derived from alignment at M-pyrifera_M_contig80566:3..506+ (Macrocystis pyrifera P11B4 male)
TCGCTGGAGGAGTTGTGTTTGGGGTGCACCAAGAGGATAAGGGTGACGCG CAAGAGAGCCAGCGAGAGTGGCGATTTGCGGGATGATTCCAAGATTCTGG AGATTCGAGTGAAACCAGGGTGGAAGGCAGGCACGAAAATCACCTTTGAG GGCGAAGGAGATGAGGCGAGAGGCGTCTTGCCAGGTGACATTGTCTTTGT CGTGGCTGAAAAGGCACATGACAAGTTCAAACGAGACGGAGACGACCTCA TCCTCAACCAACGCGTCTCACTCCTCCAAGCCCTCACCGAATGCACCGTA GAAGTCCCCACCCTCGACGGAAGAACCATCCCCGTCGCTTGCAACGAGGT CATCTCCCCAAACTACACCAAACGCATCTCCGGGGAGGGAATGGTCAACT CAAAGTCTGGCAGGAAAGGCGACATCGTCATCAAGTTCCAGATCGACTTC CCAGCACACCTCTCCGAAGAGCGGAGGGCACTCGTCAGAAGGGCGTTGGG GTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig80566:3..506+

>mRNA_M-pyrifera_M_contig80566.18868.1 ID=mRNA_M-pyrifera_M_contig80566.18868.1|Name=mRNA_M-pyrifera_M_contig80566.18868.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1008bp|location=Sequence derived from alignment at M-pyrifera_M_contig80566:3..506+ (Macrocystis pyrifera P11B4 male)
TCGCTGGAGGAGTTGTGTTTGGGGTGCACCAAGAGGATAAGGGTGACGCG
CAAGAGAGCCAGCGAGAGTGGCGATTTGCGGGATGATTCCAAGATTCTGG
AGATTCGAGTGAAACCAGGGTGGAAGGCAGGCACGAAAATCACCTTTGAG
GGCGAAGGAGATGAGGCGAGAGGCGTCTTGCCAGGTGACATTGTCTTTGT
CGTGGCTGAAAAGGCACATGACAAGTTCAAACGAGACGGAGACGACCTCA
TCCTCAACCAACGCGTCTCACTCCTCCAAGCCCTCACCGAATGCACCGTA
GAAGTCCCCACCCTCGACGGAAGAACCATCCCCGTCGCTTGCAACGAGGT
CATCTCCCCAAACTACACCAAACGCATCTCCGGGGAGGGAATGGTCAACT
CAAAGTCTGGCAGGAAAGGCGACATCGTCATCAAGTTCCAGATCGACTTC
CCAGCACACCTCTCCGAAGAGCGGAGGGCACTCGTCAGAAGGGCGTTGGG
GTGATCGCTGGAGGAGTTGTGTTTGGGGTGCACCAAGAGGATAAGGGTGA
CGCGCAAGAGAGCCAGCGAGAGTGGCGATTTGCGGGATGATTCCAAGATT
CTGGAGATTCGAGTGAAACCAGGGTGGAAGGCAGGCACGAAAATCACCTT
TGAGGGCGAAGGAGATGAGGCGAGAGGCGTCTTGCCAGGTGACATTGTCT
TTGTCGTGGCTGAAAAGGCACATGACAAGTTCAAACGAGACGGAGACGAC
CTCATCCTCAACCAACGCGTCTCACTCCTCCAAGCCCTCACCGAATGCAC
CGTAGAAGTCCCCACCCTCGACGGAAGAACCATCCCCGTCGCTTGCAACG
AGGTCATCTCCCCAAACTACACCAAACGCATCTCCGGGGAGGGAATGGTC
AACTCAAAGTCTGGCAGGAAAGGCGACATCGTCATCAAGTTCCAGATCGA
CTTCCCAGCACACCTCTCCGAAGAGCGGAGGGCACTCGTCAGAAGGGCGT
TGGGGTGA
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