mRNA_M-pyrifera_M_contig784.18427.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig784.18427.1
Unique NamemRNA_M-pyrifera_M_contig784.18427.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig784.18427.1 vs. uniprot
Match: D8LBE9_ECTSI (Ferredoxin n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LBE9_ECTSI)

HSP 1 Score: 211 bits (537), Expect = 1.200e-61
Identity = 119/161 (73.91%), Postives = 138/161 (85.71%), Query Frame = 3 
Query:    6 KSFSFLAVVLLSLLFDVCSGFVVPSTSALNVRQSPQSGGMQMFXXXXXXXXXXXXSKGIVVKVQQKTGFKETELVLQGKTNLRKALLNNKIDVYPLQGKIYNCGGGGSCGTCAVNVVAGASNCSPKGPGEKKLLDK--KPATYRLSCCTMVSGPVTIKTKP 482
            KSF F+A+++L+ +  + +GFVV  +S+LNVR+   SG   +F XXXXXXXXXXX   IVVKVQQKTGFKETEL L GKTNLRKAL++NKID+YPLQGKIYNCGGGGSCGTCAVNV AGA NCSPKGPGEKKLLDK  +PA+YRLSCCTMVSGPVT++TKP
Sbjct:    2 KSFLFVALIVLAAVLQISNGFVVAPSSSLNVRRQSHSGMQMLFGXXXXXXXXXXX---IVVKVQQKTGFKETELTLAGKTNLRKALMDNKIDIYPLQGKIYNCGGGGSCGTCAVNVKAGAQNCSPKGPGEKKLLDKNKRPASYRLSCCTMVSGPVTVQTKP 159
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BLAST of mRNA_M-pyrifera_M_contig784.18427.1 vs. uniprot
Match: A0A836CNT2_9STRA (Ferredoxin n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CNT2_9STRA)

HSP 1 Score: 149 bits (376), Expect = 1.480e-38
Identity = 72/104 (69.23%), Postives = 83/104 (79.81%), Query Frame = 3 
Query:  171 SKGIVVKVQQKTGFKETELVLQGKTNLRKALLNNKIDVYPLQGKIYNCGGGGSCGTCAVNVVAGASNCSPKGPGEKKLLDKKPATYRLSCCTMVSGPVTIKTKP 482
            SKGI V VQQK  FK+T + ++   NLRKAL+   I++YPLQGKIYNCGGGGSCGTC VNVVAG +NCSPKGP EKKLL  KP TYRLSCCT V+G +T+ TKP
Sbjct:   53 SKGIEVTVQQKY-FKDTVVAMEQPGNLRKALMGAGIEIYPLQGKIYNCGGGGSCGTCTVNVVAGGNNCSPKGPAEKKLLGNKPETYRLSCCTKVNGNITVATKP 155
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BLAST of mRNA_M-pyrifera_M_contig784.18427.1 vs. uniprot
Match: A0A7S2UXM9_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UXM9_9STRA)

HSP 1 Score: 133 bits (335), Expect = 7.360e-33
Identity = 75/155 (48.39%), Postives = 91/155 (58.71%), Query Frame = 3 
Query:   18 FLAVVLLSLLFDVCSGFVVPSTSALNVRQSPQSGGMQMFXXXXXXXXXXXXSKGIVVKVQQKTGFKETELVLQGKTNLRKALLNNKIDVYPLQGKIYNCGGGGSCGTCAVNVVAGASNCSPKGPGEKKLLDKKPATYRLSCCTMVSGPVTIKTKP 482
            F  V+ L       S F+  S  ALN  Q+  S  +QM                I VK +   GF + +L  +G  NLRK L+  KID+YPLQGK+ NCGGGG CGTCAV V+ GA+N SPK P EKK+L KKP  YRLSCCT VSG  T++TKP
Sbjct:    3 FTVVIFLVCAISGASAFLGQSL-ALNRVQASSSTSLQMLFGSKKKAAPVKGKYSITVKQR---GFTDFDLETEGTANLRKTLMAQKIDIYPLQGKLTNCGGGGVCGTCAVEVMDGANNLSPKAPNEKKVLSKKPENYRLSCCTRVSGACTVRTKP 153
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BLAST of mRNA_M-pyrifera_M_contig784.18427.1 vs. uniprot
Match: W7TM71_9STRA (Ferredoxin n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7TM71_9STRA)

HSP 1 Score: 117 bits (292), Expect = 6.870e-27
Identity = 61/106 (57.55%), Postives = 72/106 (67.92%), Query Frame = 3 
Query:  174 KGIVVKVQQKTGFKETELVLQG--KTNLRKALLNNKIDVYPLQGKIYNCGGGGSCGTCAVNVVAGASNCSPKGPGEKKLLD-KKPATYRLSCCTMVSGPVTIKTKP 482
            K   V VQQK  +K  EL ++     NLRK L+ NKID+YPLQGKIYNCGGGG CGTCA+ ++ GA N +PK P E K L  KK    RL+CCT  SG VT+KTKP
Sbjct:   54 KTFTVTVQQKF-YKNAELEVEAGAPVNLRKELMKNKIDIYPLQGKIYNCGGGGQCGTCAIQIIKGAKNLNPKSPVEVKALGGKKGDDIRLACCTRASGDVTVKTKP 158
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BLAST of mRNA_M-pyrifera_M_contig784.18427.1 vs. uniprot
Match: A0A2T1LXP7_9CHRO (Iron ABC transporter substrate-binding protein n=2 Tax=Chroococcales TaxID=1118 RepID=A0A2T1LXP7_9CHRO)

HSP 1 Score: 108 bits (270), Expect = 1.180e-24
Identity = 52/94 (55.32%), Postives = 66/94 (70.21%), Query Frame = 3 
Query:  204 TGFKETELVLQGK-TNLRKALLNNKIDVYPLQGKIYNCGGGGSCGTCAVNVVAGASNCSPKGPGEKKLLDKKPATYRLSCCTMVSGPVTIKTKP 482
            T  KE +LV+     NLR+  L N ID+Y L+GK+ NCGG G CGTC V +V G  N SPK P EK++L KKP TYRL+C  +V+GP+T+KTKP
Sbjct:    5 TFIKENKLVVAADGANLREKALQNGIDIYTLKGKLMNCGGYGQCGTCVVEIVEGLENLSPKTPFEKRVLRKKPQTYRLACQALVNGPITVKTKP 98
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BLAST of mRNA_M-pyrifera_M_contig784.18427.1 vs. uniprot
Match: UPI00055BF685 ((2Fe-2S)-binding protein n=1 Tax=Myxosarcina sp. GI1 TaxID=1541065 RepID=UPI00055BF685)

HSP 1 Score: 102 bits (253), Expect = 2.850e-22
Identity = 46/88 (52.27%), Postives = 62/88 (70.45%), Query Frame = 3 
Query:  219 TELVLQGKTNLRKALLNNKIDVYPLQGKIYNCGGGGSCGTCAVNVVAGASNCSPKGPGEKKLLDKKPATYRLSCCTMVSGPVTIKTKP 482
            TE+V    +NLR+  L N +D+Y L+GK+ NCGG G C TC V++V G  N SP+   E+K L KKP +YRLSC T+V GP+T++TKP
Sbjct:   17 TEVVAANGSNLREKALQNNVDIYKLKGKLTNCGGVGQCSTCIVDIVEGMENLSPRTDFEQKRLGKKPDSYRLSCQTVVKGPITVRTKP 104
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BLAST of mRNA_M-pyrifera_M_contig784.18427.1 vs. uniprot
Match: A0YK96_LYNSP (Probable ferredoxin n=3 Tax=Oscillatoriales TaxID=1150 RepID=A0YK96_LYNSP)

HSP 1 Score: 100 bits (250), Expect = 6.090e-22
Identity = 48/87 (55.17%), Postives = 59/87 (67.82%), Query Frame = 3 
Query:  222 ELVLQGKTNLRKALLNNKIDVYPLQGKIYNCGGGGSCGTCAVNVVAGASNCSPKGPGEKKLLDKKPATYRLSCCTMVSGPVTIKTKP 482
            E++     NLR   L  +ID+Y   GK+ NCGG G CGTC V VV G  N SP+   EK++L KKP +YRL+C TMV+GPVTIKTKP
Sbjct:   12 EIIAADGANLRIKALEKQIDLYTFMGKMMNCGGYGQCGTCIVEVVEGMENLSPRTDTEKRILKKKPDSYRLACQTMVNGPVTIKTKP 98
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BLAST of mRNA_M-pyrifera_M_contig784.18427.1 vs. uniprot
Match: M1VFK1_CYAM1 (2Fe-2S ferredoxin-type domain-containing protein n=1 Tax=Cyanidioschyzon merolae (strain NIES-3377 / 10D) TaxID=280699 RepID=M1VFK1_CYAM1)

HSP 1 Score: 103 bits (256), Expect = 1.250e-21
Identity = 45/82 (54.88%), Postives = 61/82 (74.39%), Query Frame = 3 
Query:  243 TNLRKALLNNKIDVYPLQGKIYNCGGGGSCGTCAVNVVAGASNCSPKGPGEKKLLDKK--PATYRLSCCTMVSGPVTIKTKP 482
            +NLRK LL NKIDVY L+GK+ NCGGGG CGTC V++  G  N +P+G  E ++ + K  P ++RLSCCT + GP+T++TKP
Sbjct:  109 SNLRKVLLENKIDVYTLRGKLTNCGGGGQCGTCIVDITEGQYNTNPRGWREARIFENKQAPDSWRLSCCTKIEGPITVRTKP 190
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BLAST of mRNA_M-pyrifera_M_contig784.18427.1 vs. uniprot
Match: A0A0V7ZXI1_9CYAN (Iron ABC transporter substrate-binding protein n=1 Tax=Mastigocoleus testarum BC008 TaxID=371196 RepID=A0A0V7ZXI1_9CYAN)

HSP 1 Score: 98.6 bits (244), Expect = 3.960e-21
Identity = 45/89 (50.56%), Postives = 59/89 (66.29%), Query Frame = 3 
Query:  216 ETELVLQGKTNLRKALLNNKIDVYPLQGKIYNCGGGGSCGTCAVNVVAGASNCSPKGPGEKKLLDKKPATYRLSCCTMVSGPVTIKTKP 482
            + E+V     NLR   + N +D+Y + GK+ NCGG G CGTCAV +V+G  N SP+   E KLL KKP  YRL+C T+V+GPV + TKP
Sbjct:   10 DKEVVAATGANLRLKAMENSVDIYKMWGKMMNCGGYGQCGTCAVEIVSGMENLSPRTDAENKLLKKKPDNYRLACQTLVNGPVEVITKP 98
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BLAST of mRNA_M-pyrifera_M_contig784.18427.1 vs. uniprot
Match: A0A835YVE2_9STRA (2Fe-2S ferredoxin-type domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YVE2_9STRA)

HSP 1 Score: 99.0 bits (245), Expect = 5.160e-21
Identity = 52/105 (49.52%), Postives = 66/105 (62.86%), Query Frame = 3 
Query:  171 SKGIVVKVQQKTGFKETELV-LQGKTNLRKALLNNKIDVYPLQGKIYNCGGGGSCGTCAVNVVAGASNCSPKGPGEKKLLDKKPATYRLSCCTMVSGPVTIKTKP 482
            SKG+ V V Q  G K  + +      NLRK LL+NK+D+Y    K+  CGGGG+CGTC V V  G  NCS KG GE+K+L    A  RL+CCT V+GPV++  KP
Sbjct:   16 SKGVSVTVSQ--GSKVLKQIDSDAPVNLRKLLLDNKVDLYKPLDKLKQCGGGGTCGTCRVRVTEGMENCSKKGLGEEKMLKAVGADIRLACCTRVTGPVSVTVKP 118
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig784.18427.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LBE9_ECTSI1.200e-6173.91Ferredoxin n=1 Tax=Ectocarpus siliculosus TaxID=28... [more]
A0A836CNT2_9STRA1.480e-3869.23Ferredoxin n=1 Tax=Tribonema minus TaxID=303371 Re... [more]
A0A7S2UXM9_9STRA7.360e-3348.39Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
W7TM71_9STRA6.870e-2757.55Ferredoxin n=2 Tax=Monodopsidaceae TaxID=425072 Re... [more]
A0A2T1LXP7_9CHRO1.180e-2455.32Iron ABC transporter substrate-binding protein n=2... [more]
UPI00055BF6852.850e-2252.27(2Fe-2S)-binding protein n=1 Tax=Myxosarcina sp. G... [more]
A0YK96_LYNSP6.090e-2255.17Probable ferredoxin n=3 Tax=Oscillatoriales TaxID=... [more]
M1VFK1_CYAM11.250e-2154.882Fe-2S ferredoxin-type domain-containing protein n... [more]
A0A0V7ZXI1_9CYAN3.960e-2150.56Iron ABC transporter substrate-binding protein n=1... [more]
A0A835YVE2_9STRA5.160e-2149.522Fe-2S ferredoxin-type domain-containing protein n... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig784contigM-pyrifera_M_contig784:1002..3136 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score102.4
Seed ortholog evalue1.5e-19
Seed eggNOG ortholog45157.CME070CT
Hectar predicted targeting categoryother localisation
EggNOG free text desc.2Fe-2S iron-sulfur cluster binding domain
EggNOG OGs2RXME@2759,COG0633@1
Ec32 ortholog description2Fe-2S ferredoxin-type domain
Ec32 orthologEc-27_004210.1
COG Functional cat.C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons2
Model size1911
Cds size363
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig784.18427.1prot_M-pyrifera_M_contig784.18427.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig784 1124..1710 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816685.9845486-UTR-M-pyrifera_M_contig784:1001..11231622816685.9845486-UTR-M-pyrifera_M_contig784:1001..1123Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig784 1002..1123 +
1692278119.089809-UTR-M-pyrifera_M_contig784:1001..11231692278119.089809-UTR-M-pyrifera_M_contig784:1001..1123Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig784 1002..1123 +
1622816686.0183659-UTR-M-pyrifera_M_contig784:1710..31361622816686.0183659-UTR-M-pyrifera_M_contig784:1710..3136Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig784 1711..3136 +
1692278119.123025-UTR-M-pyrifera_M_contig784:1710..31361692278119.123025-UTR-M-pyrifera_M_contig784:1710..3136Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig784 1711..3136 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816685.9958966-CDS-M-pyrifera_M_contig784:1123..12971622816685.9958966-CDS-M-pyrifera_M_contig784:1123..1297Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig784 1124..1297 +
1692278119.1014335-CDS-M-pyrifera_M_contig784:1123..12971692278119.1014335-CDS-M-pyrifera_M_contig784:1123..1297Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig784 1124..1297 +
1622816686.006778-CDS-M-pyrifera_M_contig784:1521..17101622816686.006778-CDS-M-pyrifera_M_contig784:1521..1710Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig784 1522..1710 +
1692278119.1118617-CDS-M-pyrifera_M_contig784:1521..17101692278119.1118617-CDS-M-pyrifera_M_contig784:1521..1710Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig784 1522..1710 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig784.18427.1

>prot_M-pyrifera_M_contig784.18427.1 ID=prot_M-pyrifera_M_contig784.18427.1|Name=mRNA_M-pyrifera_M_contig784.18427.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=121bp
MQMFFGGGGAKKGAAASKGIVVKVQQKTGFKETELVLQGKTNLRKALLNN
KIDVYPLQGKIYNCGGGGSCGTCAVNVVAGASNCSPKGPGEKKLLDKKPA
TYRLSCCTMVSGPVTIKTKP*
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mRNA from alignment at M-pyrifera_M_contig784:1002..3136+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig784.18427.1 ID=mRNA_M-pyrifera_M_contig784.18427.1|Name=mRNA_M-pyrifera_M_contig784.18427.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=2135bp|location=Sequence derived from alignment at M-pyrifera_M_contig784:1002..3136+ (Macrocystis pyrifera P11B4 male)
TGAAGAAGAGCTTCTCCTTTCTCGCGGTGGTTCTCCTCTCGTTGTTGTTC
GACGTTTGCAGTGGGTTTGTGGTTCCCTCAACGTCGGCACTCAATGTGCG
GCAGTCACCCCAAAGCGGGGGTATGCAAATGTTTTTCGGAGGTGGAGGTG
CCAAGAAGGGAGCGGCTGCCAGCAAGGGTATCGTGGTGAAGGTGCAGCAA
AAGACCGGCTTTAAGGAGACGGAGCTTGTGCTTCAGGGCAAGACGAACCT
GAGGAAGGCACTGCTCAATAACAAGATCGACGTGTACCCGCTGCAGGCGA
GTTTCATGTCTTGATGTCTCCATTGGAATTTACTTCGAACCGCGTACGTG
TGCGAACACGTCGATCATGTTTTTTTTCAACCTGTCAGGAATGATATCAT
AGGAGTGTACACTGCTGTGGCCGGTCCTTTTTCTTTCATGCTTCCGCAAA
ACTTCACAAATCGTCCTCGCTCGCCACAGCCAGACCGCTGACCAATTTAT
TCCATGATACTCTGTTGCAGGGTAAAATTTATAACTGCGGGGGTGGTGGC
TCTTGTGGCACGTGCGCCGTTAACGTGGTAGCCGGAGCGTCAAACTGCAG
CCCTAAAGGGCCGGGGGAGAAAAAGCTGCTGGACAAGAAACCGGCGACAT
ACAGGCTTTCGTGTTGCACGATGGTGTCCGGACCCGTTACGATCAAGACC
AAGCCATAGGTCATGGCTGGCGAATGTGCTGCCTCCCGAAAGCGGGGACT
CATTTTTGTTTTCAGGGTCGGTCGCAATGTTCTCTTTGGCTTGAGCTTGT
GCTTTGACTTCGCCAGTATCACATAACACAGGTGTTTTTTCGAATGTCGG
GTCGCGAACCGTGGGGATGTCTATTCGTAGACCTGGCTGAATCGAACCGA
CGAAGGCACCAGTTTCCTATGTTTTCCATATAGGCATATAGGCGTTCGAG
TGCGCGATTAGGTGTCCATAATGTGAGCAGGGACGTGGCTGGGTTATCCT
TTGCTGTAGTACTCTTTGTCGCCGTCTACGGATTACAGGTAGAGAGCTGT
GGATTTTGCTCTTTATGTAGACGCGCTTGACAACGCTCAGTTTTCGAGCC
TTCAGCTCTTGAACTCTCAACACAGCAGCATTAGAGACGAATTTAATGCT
ACGAACACAAACACAGTAGAGGCAATTATACAGCCAGGAGCGCTGGAGTT
GTGGGTGGGTGCCAACGCAAATCGTTATCTGAACACGTTTTTTTGCATCA
AGTGCTTAATATTTTATTATATCATACGTCGTCACACCGTGTCTTGCGTC
AAGCGAATGGACAACAGCAGTGCGTTTCTCTCTTCATATTCCATCAATCC
CATCATCATTACTGAGGGGCAAACTCGTGAAATGAACTTTCTAAGACCCC
CCGCGCACCAAACCCACGCTATCATATCATACGCCGGATGAACCACAATG
CGTACAAGCGTCACATCTTACAAAACGACCGTCACATGACAATACTTGAA
ACGCCAATCGCGCGAATAGGCTCTGTCGCAGCGCTGTGGAAGATGAGCTA
GAAGAAAAGTGGACGCATTTCCCTTTGCGCCACCTGCCCTTCCTATTACG
CGCGTTTCCCCCCGTTACAACGATTGCTATCTCGTGCCCACGTTCGCAGT
TCTCAGAACAAAGGCCTACTCAATACAGCCATCGGCTTCATCCCGAATGG
AAAAATTACAAAGACTGAAGCTTTTGGTGCTTTGGACCGAAACACCTCGC
ATAGGCCACGCCGACAGCTCTTACAATCTTTTTTTACACAGGCCTTAGAC
TGGCCGCCACACGGGTCCGGGTATTTGATCCGCTGACGAGGGGCAATGAA
CGACCATTCCTCTACACACCAGTTTCATCCCAAATACGCTCTGGACTTCT
TCGTTTGGTCTGTATACCTGGGCGTCTCGAACACCACGTTCAAGATGCTG
CCTCAAGACCAAGGACGACCGACATCGCCTCGGCGGGGGATACCCTGCAC
CCCACTGCCCGCTTCCATGCGCATGTCGTCGTCAGCGTCTACCGCAGTCT
CCATTTCACGCACGTTTTCGTTCGCGTAAGCTGAAACGCGGGGGTCCTCC
TGACTAGCGCGGCCCGCCTTCCTCGACACGGGGAC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig784:1002..3136+

>mRNA_M-pyrifera_M_contig784.18427.1 ID=mRNA_M-pyrifera_M_contig784.18427.1|Name=mRNA_M-pyrifera_M_contig784.18427.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=726bp|location=Sequence derived from alignment at M-pyrifera_M_contig784:1002..3136+ (Macrocystis pyrifera P11B4 male)
ATGCAAATGTTTTTCGGAGGTGGAGGTGCCAAGAAGGGAGCGGCTGCCAG
CAAGGGTATCGTGGTGAAGGTGCAGCAAAAGACCGGCTTTAAGGAGACGG
AGCTTGTGCTTCAGGGCAAGACGAACCTGAGGAAGGCACTGCTCAATAAC
AAGATCGACGTGTACCCGCTGCAGATGCAAATGTTTTTCGGAGGTGGAGG
TGCCAAGAAGGGAGCGGCTGCCAGCAAGGGTATCGTGGTGAAGGTGCAGC
AAAAGACCGGCTTTAAGGAGACGGAGCTTGTGCTTCAGGGCAAGACGAAC
CTGAGGAAGGCACTGCTCAATAACAAGATCGACGTGTACCCGCTGCAGGG
TAAAATTTATAACTGCGGGGGTGGTGGCTCTTGTGGCACGTGCGCCGTTA
ACGTGGTAGCCGGAGCGTCAAACTGCAGCCCTAAAGGGCCGGGGGAGAAA
AAGCTGCTGGACAAGAAACCGGCGACATACAGGCTTTCGTGTTGCACGAT
GGTGTCCGGACCCGTTACGATCAAGACCAAGCCATAGGGTAAAATTTATA
ACTGCGGGGGTGGTGGCTCTTGTGGCACGTGCGCCGTTAACGTGGTAGCC
GGAGCGTCAAACTGCAGCCCTAAAGGGCCGGGGGAGAAAAAGCTGCTGGA
CAAGAAACCGGCGACATACAGGCTTTCGTGTTGCACGATGGTGTCCGGAC
CCGTTACGATCAAGACCAAGCCATAG
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