mRNA_M-pyrifera_M_contig78090.18359.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig78090.18359.1
Unique NamemRNA_M-pyrifera_M_contig78090.18359.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig78090.18359.1 vs. uniprot
Match: A0A0H5C1R2_CYBJN (ATP-dependent DNA helicase n=1 Tax=Cyberlindnera jadinii (strain ATCC 18201 / CBS 1600 / BCRC 20928 / JCM 3617 / NBRC 0987 / NRRL Y-1542) TaxID=983966 RepID=A0A0H5C1R2_CYBJN)

HSP 1 Score: 105 bits (262), Expect = 1.570e-24
Identity = 56/108 (51.85%), Postives = 70/108 (64.81%), Query Frame = 1 
Query:    1 PLVSFAFPDGSRRVERVTPFVFRAEDPREPRWYAERKQIPLALGWAVSVHKSQGMTLPNLCAHLDGTFEFGQAYVALSRAVSRQGLRVTGFHKSHVKAKPSVVQFYRK 324
            PLV F  PDG  RV  V P  +  ED ++      R Q+PL L WA+S+HKSQG TLP +  +L   FE GQAYVA+SRAVSR+GL++  F +S V A P VVQFY +
Sbjct:  227 PLVRFLTPDGQSRVVLVNPETWTVEDEKQTP-LVSRTQLPLILAWALSIHKSQGQTLPKVRVNLKRIFEKGQAYVAISRAVSREGLQILNFDQSKVFAHPRVVQFYEE 333
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BLAST of mRNA_M-pyrifera_M_contig78090.18359.1 vs. uniprot
Match: A0A1G4KDP5_9SACH (ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea mirantina TaxID=1230905 RepID=A0A1G4KDP5_9SACH)

HSP 1 Score: 102 bits (255), Expect = 3.280e-23
Identity = 57/107 (53.27%), Postives = 70/107 (65.42%), Query Frame = 1 
Query:    1 PLVSFAFPDGSRRVERVTPFVFRAEDPREPRWYAERKQIPLALGWAVSVHKSQGMTLPNLCAHLDGTFEFGQAYVALSRAVSRQGLRVTGFHKSHVKAKPSVVQFYR 321
            PLV F  PDGS R   V P  +  ED  E +    R Q+PL L WA+S+HKSQG TLP +   L   FE GQAYVALSRAVSR GL+V  F++S V+A  SV++FY+
Sbjct:  642 PLVRFLTPDGSSRCVLVRPEDWAIEDENE-KPLVSRVQLPLMLAWALSIHKSQGQTLPKVKVDLRRIFEKGQAYVALSRAVSRSGLQVLNFNRSKVQAHGSVIEFYK 747
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BLAST of mRNA_M-pyrifera_M_contig78090.18359.1 vs. uniprot
Match: A0A1G4JJ13_9SACH (ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea meyersii CBS 8951 TaxID=1266667 RepID=A0A1G4JJ13_9SACH)

HSP 1 Score: 102 bits (255), Expect = 3.300e-23
Identity = 56/107 (52.34%), Postives = 70/107 (65.42%), Query Frame = 1 
Query:    1 PLVSFAFPDGSRRVERVTPFVFRAEDPREPRWYAERKQIPLALGWAVSVHKSQGMTLPNLCAHLDGTFEFGQAYVALSRAVSRQGLRVTGFHKSHVKAKPSVVQFYR 321
            P V F  PDGS R+  V P  +  ED  + +    R Q+PL L WA+S+HKSQG TLP +   L   FE GQAYVALSRA SR+GL+V  F+KS V+A  SV++FYR
Sbjct:  700 PFVRFLTPDGSSRLVLVQPEDWAVEDEHQ-KPLVSRIQLPLMLAWALSIHKSQGQTLPKVKVDLRRIFEKGQAYVALSRATSREGLQVLNFNKSKVQAHESVIEFYR 805
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BLAST of mRNA_M-pyrifera_M_contig78090.18359.1 vs. uniprot
Match: A0A642UC18_DIURU (ATP-dependent DNA helicase PIF1 n=1 Tax=Diutina rugosa TaxID=5481 RepID=A0A642UC18_DIURU)

HSP 1 Score: 101 bits (251), Expect = 1.140e-22
Identity = 54/110 (49.09%), Postives = 68/110 (61.82%), Query Frame = 1 
Query:    1 PLVSFAFPDGSRRVERVTPFVFRAEDPREPRWYAERKQIPLALGWAVSVHKSQGMTLPNLCAHLDGTFEFGQAYVALSRAVSRQGLRVTGFHKSHVKAKPSVVQFYRKFD 330
            PLV F   DG+ R   + P  +  ED       A R QIPL L W++S+HKSQG TLP +  +L   FE GQAYVALSRAVSR+GL+V GF +  V+  P V++FY   D
Sbjct:  724 PLVQFRTNDGNFRYHLMEPEKWSVED-ETGHVLASRSQIPLILAWSLSIHKSQGQTLPLVKVNLASCFEMGQAYVALSRAVSREGLQVIGFSRDRVRCHPKVLEFYSNLD 832
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BLAST of mRNA_M-pyrifera_M_contig78090.18359.1 vs. uniprot
Match: A0A0P1KKU7_9SACH (ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea quebecensis TaxID=1654605 RepID=A0A0P1KKU7_9SACH)

HSP 1 Score: 101 bits (251), Expect = 1.140e-22
Identity = 56/107 (52.34%), Postives = 70/107 (65.42%), Query Frame = 1 
Query:    1 PLVSFAFPDGSRRVERVTPFVFRAEDPREPRWYAERKQIPLALGWAVSVHKSQGMTLPNLCAHLDGTFEFGQAYVALSRAVSRQGLRVTGFHKSHVKAKPSVVQFYR 321
            PLV F  PDG+ R   V P  +  ED ++ +    R Q+PL L WA+S+HKSQG TLP +   L   FE GQAYVALSRAVSR GL+V  F+K+ V+A  SV+ FYR
Sbjct:  707 PLVRFLTPDGTSRCVLVQPEDWAVEDEQQ-KPIVSRVQLPLMLAWALSIHKSQGQTLPKVKVDLRRIFEKGQAYVALSRAVSRDGLQVLNFNKAKVQAHSSVMDFYR 812
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BLAST of mRNA_M-pyrifera_M_contig78090.18359.1 vs. uniprot
Match: K0KM86_WICCF (ATP-dependent DNA helicase PIF1 n=1 Tax=Wickerhamomyces ciferrii (strain ATCC 14091 / BCRC 22168 / CBS 111 / JCM 3599 / NBRC 0793 / NRRL Y-1031 F-60-10) TaxID=1206466 RepID=K0KM86_WICCF)

HSP 1 Score: 101 bits (251), Expect = 1.150e-22
Identity = 54/108 (50.00%), Postives = 68/108 (62.96%), Query Frame = 1 
Query:    1 PLVSFAFPDGSRRVERVTPFVFRAEDPREPRWYAERKQIPLALGWAVSVHKSQGMTLPNLCAHLDGTFEFGQAYVALSRAVSRQGLRVTGFHKSHVKAKPSVVQFYRK 324
            PLV F  PDG  R   V P ++   D  E R    R Q+PL L WA+S+HKSQG TLP +  +L   FE GQAYVALSRAVSR+GL+V  F +  + A P V++FY +
Sbjct:  817 PLVKFLTPDGQSRTILVQPEIWEVSDEFE-RPLVTRMQVPLILAWALSIHKSQGQTLPRVRVNLKKIFEKGQAYVALSRAVSREGLQVLFFDEKKIWAHPKVIEFYNR 923
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BLAST of mRNA_M-pyrifera_M_contig78090.18359.1 vs. uniprot
Match: A0A0C7N4Y2_9SACH (ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea lanzarotensis TaxID=1245769 RepID=A0A0C7N4Y2_9SACH)

HSP 1 Score: 100 bits (250), Expect = 1.560e-22
Identity = 55/107 (51.40%), Postives = 69/107 (64.49%), Query Frame = 1 
Query:    1 PLVSFAFPDGSRRVERVTPFVFRAEDPREPRWYAERKQIPLALGWAVSVHKSQGMTLPNLCAHLDGTFEFGQAYVALSRAVSRQGLRVTGFHKSHVKAKPSVVQFYR 321
            P V F  PDGS R+  V P  +  ED  + +    R Q+PL L WA+S+HKSQG TLP +   L   FE GQAYVALSRA  R+GL+V  F+KS V+A  SV++FYR
Sbjct:  681 PFVRFLTPDGSSRLVLVQPEDWAVEDEHQ-KPLVSRIQLPLMLAWALSIHKSQGQTLPKVKVDLRRIFEKGQAYVALSRATCREGLQVLNFNKSKVQAHESVIEFYR 786
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BLAST of mRNA_M-pyrifera_M_contig78090.18359.1 vs. uniprot
Match: A0A1G4M6E1_LACFM (ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea fermentati TaxID=4955 RepID=A0A1G4M6E1_LACFM)

HSP 1 Score: 100 bits (249), Expect = 2.140e-22
Identity = 56/107 (52.34%), Postives = 69/107 (64.49%), Query Frame = 1 
Query:    1 PLVSFAFPDGSRRVERVTPFVFRAEDPREPRWYAERKQIPLALGWAVSVHKSQGMTLPNLCAHLDGTFEFGQAYVALSRAVSRQGLRVTGFHKSHVKAKPSVVQFYR 321
            PLV F  PDGS R   V P  +  ED  E +    R Q+PL L WA+S+HKSQG TLP +   L   FE GQAYVALSRAVSR+GL+V  F +  V+A  +VV+FY+
Sbjct:  762 PLVRFLTPDGSSRCVLVQPEDWAVEDENE-KPLVSRVQLPLMLAWALSIHKSQGQTLPKVKVDLRRIFEKGQAYVALSRAVSREGLQVLNFSRHKVQAHDAVVKFYK 867
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BLAST of mRNA_M-pyrifera_M_contig78090.18359.1 vs. uniprot
Match: A0A1G4K2B9_9SACH (ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea sp. CBS 6924 TaxID=433476 RepID=A0A1G4K2B9_9SACH)

HSP 1 Score: 100 bits (248), Expect = 2.900e-22
Identity = 55/107 (51.40%), Postives = 69/107 (64.49%), Query Frame = 1 
Query:    1 PLVSFAFPDGSRRVERVTPFVFRAEDPREPRWYAERKQIPLALGWAVSVHKSQGMTLPNLCAHLDGTFEFGQAYVALSRAVSRQGLRVTGFHKSHVKAKPSVVQFYR 321
            P V F  PDGS R+  V P  +  ED  + +    R Q+PL L WA+S+HKSQG TLP +   L   FE GQAYVALSRA  R+GL+V  F+K+ V+A  SVV+FYR
Sbjct:  679 PFVRFLTPDGSSRLVLVQPEDWAVEDEHQ-KPLVSRIQLPLMLAWALSIHKSQGQTLPKVKVDLRRIFEKGQAYVALSRATCREGLQVLNFNKTKVQAHESVVEFYR 784
[Close]
BLAST of mRNA_M-pyrifera_M_contig78090.18359.1 vs. uniprot
Match: A0A1G4KK60_9SACH (ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea nothofagi CBS 11611 TaxID=1266666 RepID=A0A1G4KK60_9SACH)

HSP 1 Score: 99.8 bits (247), Expect = 3.960e-22
Identity = 55/107 (51.40%), Postives = 70/107 (65.42%), Query Frame = 1 
Query:    1 PLVSFAFPDGSRRVERVTPFVFRAEDPREPRWYAERKQIPLALGWAVSVHKSQGMTLPNLCAHLDGTFEFGQAYVALSRAVSRQGLRVTGFHKSHVKAKPSVVQFYR 321
            P V F  PDGS R+  V P  +  ED  + +    R Q+PL L WA+S+HKSQG TLP +   L   FE GQAYVALSRAVSR GL+V  F+K+ V+A  SV++FY+
Sbjct:  693 PFVRFLTPDGSSRLVLVQPEDWAIEDEHQ-KPLVSRIQLPLMLAWALSIHKSQGQTLPKVKVDLRRIFEKGQAYVALSRAVSRDGLQVLNFNKTKVQAHESVMEFYQ 798
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig78090.18359.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A0H5C1R2_CYBJN1.570e-2451.85ATP-dependent DNA helicase n=1 Tax=Cyberlindnera j... [more]
A0A1G4KDP5_9SACH3.280e-2353.27ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea ... [more]
A0A1G4JJ13_9SACH3.300e-2352.34ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea ... [more]
A0A642UC18_DIURU1.140e-2249.09ATP-dependent DNA helicase PIF1 n=1 Tax=Diutina ru... [more]
A0A0P1KKU7_9SACH1.140e-2252.34ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea ... [more]
K0KM86_WICCF1.150e-2250.00ATP-dependent DNA helicase PIF1 n=1 Tax=Wickerhamo... [more]
A0A0C7N4Y2_9SACH1.560e-2251.40ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea ... [more]
A0A1G4M6E1_LACFM2.140e-2252.34ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea ... [more]
A0A1G4K2B9_9SACH2.900e-2251.40ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea ... [more]
A0A1G4KK60_9SACH3.960e-2251.40ATP-dependent DNA helicase PIF1 n=1 Tax=Lachancea ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig78090contigM-pyrifera_M_contig78090:115..444 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeFungi
Seed ortholog score103.2
Seed ortholog evalue7.8e-20
Seed eggNOG ortholog1041607.K0KM86
Preferred namePIF1
KEGG koko:K03507,ko:K15255
Hectar predicted targeting categoryother localisation
GOsGO:0000002,GO:0000166,GO:0000228,GO:0000278,GO:0000722,GO:0000723,GO:0000724,GO:0000725,GO:0000727,GO:0000733,GO:0000781,GO:0000784,GO:0000785,GO:0000790,GO:0000792,GO:0003674,GO:0003676,GO:0003677,GO:0003678,GO:0003697,GO:0003824,GO:0004003,GO:0004386,GO:0004857,GO:0005488,GO:0005524,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005657,GO:0005694,GO:0005720,GO:0005724,GO:0005730,GO:0005737,GO:0005739,GO:0005740,GO:0006139,GO:0006259,GO:0006260,GO:0006261,GO:0006271,GO:0006273,GO:0006281,GO:0006302,GO:0006310,GO:0006312,GO:0006401,GO:0006725,GO:0006807,GO:0006950,GO:0006974,GO:0006996,GO:0007005,GO:0007049,GO:0008026,GO:0008094,GO:0008144,GO:0008150,GO:0008152,GO:0009056,GO:0009057,GO:0009058,GO:0009059,GO:0009889,GO:0009890,GO:0009892,GO:0009987,GO:0010521,GO:0010556,GO:0010558,GO:0010605,GO:0010638,GO:0010639,GO:0016020,GO:0016043,GO:0016070,GO:0016462,GO:0016787,GO:0016817,GO:0016818,GO:0016887,GO:0017076,GO:0017111,GO:0017116,GO:0019219,GO:0019222,GO:0019439,GO:0022402,GO:0022616,GO:0030234,GO:0030554,GO:0030894,GO:0031090,GO:0031297,GO:0031323,GO:0031324,GO:0031326,GO:0031327,GO:0031933,GO:0031966,GO:0031967,GO:0031974,GO:0031975,GO:0031981,GO:0032042,GO:0032200,GO:0032204,GO:0032205,GO:0032210,GO:0032211,GO:0032392,GO:0032508,GO:0032553,GO:0032555,GO:0032559,GO:0032991,GO:0032993,GO:0033043,GO:0033044,GO:0033260,GO:0033553,GO:0033554,GO:0033567,GO:0034641,GO:0034645,GO:0034655,GO:0035639,GO:0035861,GO:0036094,GO:0042592,GO:0042623,GO:0043086,GO:0043137,GO:0043138,GO:0043139,GO:0043140,GO:0043141,GO:0043142,GO:0043167,GO:0043168,GO:0043170,GO:0043226,GO:0043227,GO:0043228,GO:0043229,GO:0043231,GO:0043232,GO:0043233,GO:0043504,GO:0043596,GO:0043601,GO:0044092,GO:0044237,GO:0044238,GO:0044248,GO:0044249,GO:0044260,GO:0044265,GO:0044270,GO:0044422,GO:0044424,GO:0044427,GO:0044428,GO:0044429,GO:0044444,GO:0044446,GO:0044454,GO:0044464,GO:0044786,GO:0044806,GO:0045005,GO:0045934,GO:0046483,GO:0046700,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0050789,GO:0050790,GO:0050794,GO:0050896,GO:0051052,GO:0051053,GO:0051128,GO:0051129,GO:0051130,GO:0051171,GO:0051172,GO:0051276,GO:0051338,GO:0051348,GO:0051716,GO:0051880,GO:0051972,GO:0051974,GO:0060249,GO:0060255,GO:0065007,GO:0065008,GO:0065009,GO:0070013,GO:0070035,GO:0071103,GO:0071704,GO:0071840,GO:0071932,GO:0080090,GO:0090304,GO:0090734,GO:0097159,GO:0097367,GO:0098687,GO:0098772,GO:0140097,GO:1901265,GO:1901360,GO:1901361,GO:1901363,GO:1901575,GO:1901576,GO:1902296,GO:1902319,GO:1902377,GO:1902969,GO:1902983,GO:1903047,GO:1903469,GO:1904356,GO:1904357,GO:1905465,GO:1905467,GO:1990518,GO:2000112,GO:2000113,GO:2000278,GO:2000279,GO:2001251,GO:2001252
EggNOG free text desc.DNA-dependent ATPase and 5'-3' DNA helicase required for the maintenance of both mitochondrial and nuclear genome stability. Efficiently unwinds G-quadruplex (G4) DNA structures and forked RNA-DNA hybrids. Resolves G4 structures, preventing replication pausing and double-strand breaks (DSBs) at G4 motifs. Involved in the maintenance of telomeric DNA. Inhibits telomere elongation, de novo telomere formation and telomere addition to DSBs via catalytic inhibition of telomerase. Reduces the processivity of telomerase by displacing active telomerase from DNA ends. Releases telomerase by unwinding the short telomerase RNA telomeric DNA hybrid that is the intermediate in the telomerase reaction. Involved in the maintenance of ribosomal (rDNA). Required for efficient fork arrest at the replicaion fork barrier within rDNA. Involved in the maintenance of mitochondrial (mtDNA). Required to maintain mtDNA under conditions that introduce dsDNA breaks in mtDNA, either preventing or repairing dsDNA breaks. May inhibit replication progression to allow time for repair. May have a general role in chromosomal replication by affecting Okazaki fragment maturation. May have a role in conjunction with DNA2 helicase nuclease in 5'-flap extension during Okazaki fragment processing
EggNOG OGs38G42@33154,3NTY9@4751,3QMUK@4890,3RR9I@4891,COG0507@1,KOG0987@2759
EC3.6.4.12
COG Functional cat.L
Best tax levelSaccharomycetes
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03029,ko03032
Exons1
Model size330
Cds size330
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816682.215389-CDS-M-pyrifera_M_contig78090:114..4441622816682.215389-CDS-M-pyrifera_M_contig78090:114..444Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig78090 115..444 +
1692278116.8823092-CDS-M-pyrifera_M_contig78090:114..4441692278116.8823092-CDS-M-pyrifera_M_contig78090:114..444Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig78090 115..444 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig78090.18359.1prot_M-pyrifera_M_contig78090.18359.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig78090 115..444 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig78090.18359.1

>prot_M-pyrifera_M_contig78090.18359.1 ID=prot_M-pyrifera_M_contig78090.18359.1|Name=mRNA_M-pyrifera_M_contig78090.18359.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=110bp
PLVSFAFPDGSRRVERVTPFVFRAEDPREPRWYAERKQIPLALGWAVSVH
KSQGMTLPNLCAHLDGTFEFGQAYVALSRAVSRQGLRVTGFHKSHVKAKP
SVVQFYRKFD
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mRNA from alignment at M-pyrifera_M_contig78090:115..444+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig78090.18359.1 ID=mRNA_M-pyrifera_M_contig78090.18359.1|Name=mRNA_M-pyrifera_M_contig78090.18359.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=330bp|location=Sequence derived from alignment at M-pyrifera_M_contig78090:115..444+ (Macrocystis pyrifera P11B4 male)
CCACTTGTTTCATTTGCCTTTCCTGATGGATCAAGAAGGGTTGAACGAGT
GACACCATTTGTATTTCGTGCAGAAGATCCGAGGGAACCTCGTTGGTATG
CAGAGCGAAAGCAAATTCCTCTGGCACTTGGTTGGGCCGTGTCAGTTCAT
AAGAGTCAAGGAATGACACTACCCAACCTTTGTGCACATCTTGATGGGAC
GTTTGAGTTTGGTCAAGCATACGTTGCTCTCAGTCGCGCAGTATCAAGGC
AAGGCCTGCGCGTCACTGGCTTTCACAAGAGCCATGTAAAGGCTAAACCG
AGTGTTGTACAATTCTACCGCAAATTTGAT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig78090:115..444+

>mRNA_M-pyrifera_M_contig78090.18359.1 ID=mRNA_M-pyrifera_M_contig78090.18359.1|Name=mRNA_M-pyrifera_M_contig78090.18359.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=660bp|location=Sequence derived from alignment at M-pyrifera_M_contig78090:115..444+ (Macrocystis pyrifera P11B4 male)
CCACTTGTTTCATTTGCCTTTCCTGATGGATCAAGAAGGGTTGAACGAGT
GACACCATTTGTATTTCGTGCAGAAGATCCGAGGGAACCTCGTTGGTATG
CAGAGCGAAAGCAAATTCCTCTGGCACTTGGTTGGGCCGTGTCAGTTCAT
AAGAGTCAAGGAATGACACTACCCAACCTTTGTGCACATCTTGATGGGAC
GTTTGAGTTTGGTCAAGCATACGTTGCTCTCAGTCGCGCAGTATCAAGGC
AAGGCCTGCGCGTCACTGGCTTTCACAAGAGCCATGTAAAGGCTAAACCG
AGTGTTGTACAATTCTACCGCAAATTTGATCCACTTGTTTCATTTGCCTT
TCCTGATGGATCAAGAAGGGTTGAACGAGTGACACCATTTGTATTTCGTG
CAGAAGATCCGAGGGAACCTCGTTGGTATGCAGAGCGAAAGCAAATTCCT
CTGGCACTTGGTTGGGCCGTGTCAGTTCATAAGAGTCAAGGAATGACACT
ACCCAACCTTTGTGCACATCTTGATGGGACGTTTGAGTTTGGTCAAGCAT
ACGTTGCTCTCAGTCGCGCAGTATCAAGGCAAGGCCTGCGCGTCACTGGC
TTTCACAAGAGCCATGTAAAGGCTAAACCGAGTGTTGTACAATTCTACCG
CAAATTTGAT
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