mRNA_M-pyrifera_M_contig7789.18317.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig7789.18317.1
Unique NamemRNA_M-pyrifera_M_contig7789.18317.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig7789.18317.1 vs. uniprot
Match: D8LH70_ECTSI (tRNA (guanine(26)-N(2))-dimethyltransferase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LH70_ECTSI)

HSP 1 Score: 812 bits (2098), Expect = 1.710e-293
Identity = 393/469 (83.80%), Postives = 438/469 (93.39%), Query Frame = 1
Query:    1 RSATQREGAEFTPEDLQQLESTDWGERAVESASTDGIQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAVEAAKRNIEFNQVDSSKMRVQQGDAIAAMHSSIAAKQRFDVIDLDPYGSAAPFLDAAVQAVDDGGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKGRYLHEMALRIVLNALEAQANRYRRHIVPVLSLSIDFYVRLFVRVYTSPAEVKKACTKAAYVHQSLGCGSFYLQPVGKMSKNNYNPSLSTGDGCCPQTGKRFKIGGPIWAPPMHDVEWVELLLNRVQENPGPFPPTTKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHCDSPRMIDVQAALVNAGYRVSQQHKEPQAVKTDAPDDVVWDIMRCWVKKHPVSDKRKTSSPGQAILEREPSLEADFTVPSGLWQKKAATRYQQNPEPYWGPKARATGNKRGVADGGGPADGTPAGKKKKPAES 1407
            R   +REGAEFT +DL+ L++TDW +RA +SASTDGIQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAV+AA+RNI+FNQVD SK+RVQQGDAI+AMH+S AAKQRFDVIDLDPYGSAAPFLDAAVQ V++GGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKG+YLHEMALRIVL+ALEAQANRYRRH+VPVLSLSIDFYVRLFVRV+TS AEVKKACTKAAYVHQSLGCGSF+L PVGKMS NNYNPSL+TGDG CPQTGKRFK+GGPIW+P +H++EWV LLL+RVQ+N GP+ P+TKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHC+SPRMID+QAALVNAG+RVSQQHKEPQAVKTDAPDDVVWD+MRCWVKKHPVSDKRKTSSPGQAIL ++P+ +ADFTVPSGLWQ+K ATRYQQNPEPYWGPK+RATGNKRG  +  GP++G P  K+ +  ES
Sbjct:   92 RKTRKREGAEFTAQDLEGLDATDWSQRAADSASTDGIQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAVQAAQRNIDFNQVDVSKIRVQQGDAISAMHASSAAKQRFDVIDLDPYGSAAPFLDAAVQCVEEGGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKGKYLHEMALRIVLHALEAQANRYRRHVVPVLSLSIDFYVRLFVRVFTSAAEVKKACTKAAYVHQSLGCGSFFLHPVGKMSNNNYNPSLTTGDGLCPQTGKRFKVGGPIWSPAIHNMEWVRLLLDRVQKNRGPYQPSTKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHCNSPRMIDMQAALVNAGFRVSQQHKEPQAVKTDAPDDVVWDVMRCWVKKHPVSDKRKTSSPGQAILSKDPNHQADFTVPSGLWQRKTATRYQQNPEPYWGPKSRATGNKRGAEESCGPSEGAPEDKRNRKEES 560          
BLAST of mRNA_M-pyrifera_M_contig7789.18317.1 vs. uniprot
Match: W7T9F0_9STRA (tRNA (guanine(26)-N(2))-dimethyltransferase n=2 Tax=Monodopsidaceae TaxID=425072 RepID=W7T9F0_9STRA)

HSP 1 Score: 533 bits (1373), Expect = 4.900e-183
Identity = 271/458 (59.17%), Postives = 340/458 (74.24%), Query Frame = 1
Query:   31 FTPEDLQ-QLESTDWGERAVESASTDGIQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAVEAAKRNIEFNQVDSSKMRVQQGDAIAAMHSSIAAKQRFDVIDLDPYGSAAPFLDAAVQAVDDGGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKGRYLHEMALRIVLNALEAQANRYRRHIVPVLSLSIDFYVRLFVRVYTSPAEVKKACTKAAYVHQSLGCGSFYLQPVGKMSKNNYNPSL--STGDGC---CPQTGKRFKIGGPIWAPPMHDVEWVELLLNRVQENP--------GPFPPTTKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHCDSPRMIDVQAALVNAGYRVSQQHKEPQAVKTDAPDDVVWDIMRCWVKKHPVSDK-RKTSSPGQAILEREPSLEADFTVPSGLWQ-KKAATRYQQNPEPYWGPKARATGNKRGVADGG 1356
             TPE +  QL   DW  R  E+A+ +G+ +LDALAA+ LRSIRY KE+PG+K++VVNDL+E AVE A RN+ +N+VD +++  QQG+A   M+    ++++FDVID+DPYGSAAPF+DAAVQAV +GGLLCVTCTD+ VL+G HPE C+ KY +MPTK RY+HEMA+RIVL ALE QANRYRR+IVP+LS+ IDFY+R+FVRV+TSPAEVKKA  K AYVHQSLGCGSFYLQ  GK S +  N S   STG  C   CP+TG  F++GGPIW+ PMHD+EWV   L R +E          G  P  T +R+ G+LT+VSEEL DVPL Y+L DM  TLHC  PR+ DV+AAL+NAGYRVS QHKEP+A KTDAP  V+WDIMRCWV+KHPV  + +K SSP   IL + P L+A+F+ P  L   KK  +RY  NPE +WGPK++A G KR + +GG
Sbjct:  120 LTPEQVTAQLADVDWTARVAETAAEEGLVVLDALAASGLRSIRYFKEVPGIKKIVVNDLEEPAVEQAHRNVAYNRVDPTRVAPQQGNATLVMYQHAESREQFDVIDIDPYGSAAPFMDAAVQAVANGGLLCVTCTDLAVLNGNHPEVCWGKYRAMPTKARYMHEMAVRIVLQALETQANRYRRYIVPLLSVGIDFYLRVFVRVFTSPAEVKKASLKMAYVHQSLGCGSFYLQTAGKYSGDAGNGSYQASTGPTCPPVCPETGAAFRLGGPIWSAPMHDMEWVLEALGRAEEQADADAEAKTGLAPLPTTERMVGLLTAVSEELVDVPLHYTLQDMAGTLHCTCPRVDDVKAALINAGYRVSSQHKEPRAFKTDAPPAVMWDIMRCWVQKHPVGPRGQKESSPAAKILAKAPKLQANFSWPPELLAPKKKVSRYPPNPEAFWGPKSKAKGRKRKMEEGG 577          
BLAST of mRNA_M-pyrifera_M_contig7789.18317.1 vs. uniprot
Match: A0A836C8Z8_9STRA (tRNA (guanine(26)-N(2))-dimethyltransferase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836C8Z8_9STRA)

HSP 1 Score: 516 bits (1330), Expect = 1.510e-177
Identity = 254/427 (59.48%), Postives = 322/427 (75.41%), Query Frame = 1
Query:   64 TDWGERAVESASTDGIQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAVEAAKRNIEFNQVDSSKMRVQQGDAIAAMHSSIAAKQRFDVIDLDPYGSAAPFLDAAVQAVDDGGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKGRYLHEMALRIVLNALEAQANRYRRHIVPVLSLSIDFYVRLFVRVYTSPAEVKKACTKAAYVHQSLGCGSFYLQPVGKMSKNNYNPSLSTGDGC-CPQTGKRFKIGGPIWAPPMHDVEWVELLLNRVQENPGPFPPTTKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHCDSPRMIDVQAALVNAGYRVSQQHKEPQAVKTDAPDDVVWDIMRCWVKKHPVSDKR-KTSSPGQAILEREPSLEADFTVPSGLWQKKAATRYQQNPEPYWGPKARATGNKR 1338
            T+W  RA ESA ++GI ILDALAATALRSIRY KEIPGV++VV NDLD AAVEAA++N EFN V++ K+ V QGDA   M++  + +Q+FDVIDLDPYGSAAPF+D AVQAV  GGLLCVT TDM VL G + E C+AKYGSMPTKG+YLHEMALRI+L+ L++ A +Y+RH+VPVLSLSIDFYVR+FVRV+ SP++ K A  +  YV QS  C SF++QP+ + + NN  P++   +   CPQTG   K+GGP+W  P+HD +WV  LL R++   G  PP T  RI  MLT+VSEEL DVPL Y+L D+CS LHC +P +   +AA++NAGYR+S QHK+P A+KTDAPDDV+WDIMRCWVK HPVS KR K  +    IL +EP  EADF+   G   ++ + R+ QNPEP+WGPK+RATG ++
Sbjct:   32 TNWSARAAESAKSNGIVILDALAATALRSIRYTKEIPGVRKVVANDLDAAAVEAARKNAEFNNVEAGKLDVLQGDATLLMYTRRSHEQQFDVIDLDPYGSAAPFVDGAVQAVKSGGLLCVTSTDMAVLGGANTEACFAKYGSMPTKGKYLHEMALRILLHLLDSSATKYKRHVVPVLSLSIDFYVRIFVRVFDSPSQAKAATARRGYVMQSTTCSSFFVQPMCRTAGNNLKPAVMIPEAAVCPQTGGHMKLGGPLWTAPIHDEQWVLALLARIERYKGGTPPATAPRIKSMLTTVSEELFDVPLHYNLADICSHLHCTAPPLPTFRAAVINAGYRISAQHKDPGAIKTDAPDDVIWDIMRCWVKTHPVSAKRLKPDTAAFKILSKEPKHEADFSPVQGSMLRRVS-RFHQNPEPFWGPKSRATGKRK 457          
BLAST of mRNA_M-pyrifera_M_contig7789.18317.1 vs. uniprot
Match: K3WLD8_GLOUD (tRNA (guanine(26)-N(2))-dimethyltransferase n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WLD8_GLOUD)

HSP 1 Score: 470 bits (1209), Expect = 4.840e-159
Identity = 240/452 (53.10%), Postives = 314/452 (69.47%), Query Frame = 1
Query:   22 GAEFTPEDLQQLESTDWGERAVESASTDGIQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAVEAAKRNIEFNQVDSSKMRVQQGDAIAAMHSSIAAKQRFDVIDLDPYGSAAPFLDAAVQAVDDGGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKGRYLHEMALRIVLNALEAQANRYRRHIVPVLSLSIDFYVRLFVRVYTSPAEVKKACTKAAYVHQSLGCGSFYLQPVGKMSKNNYNPSLSTGDGC-CPQTGKRFKIGGPIWAPPMHDVEWVELLLNRVQENPGPFPPTTKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHCDSPRMIDVQAALVNAGYRVSQQHKEPQAVKTDAPDDVVWDIMRCWVKKHPVSDKRK-TSSPGQAILEREPSLEADFTVPSGLWQKKAATRYQQNPEPYWGPKARATGNKRGVADGGGPADG 1371
            G EFTP++  + E         E+A + GI+I +AL+A+ LRSIRY ++IPGVK ++VND+D AAVE+ KRNIEFN++   ++   + DA   M+     K  FDVIDLDPYGSA+ FLD AVQ+V +GGLLCVTCTDM VLSG +PE C+++Y S+P K  YLHEMALRIVL ++EA AN+Y RHIVP+ + SIDFYVR+FVRVY SP  VK A TK +Y  Q +GC SF+LQP+G  S N ++ S     G  C Q   +FK+ GPIW+ P+HD + V  +  RV +    FP  TK+R+HG+LT++SEEL D PL Y+LP +  TLHC +PRM  +Q A+  AGY+ SQ HK P+A+KT+AP+DV+WDIMRCWVKKHPV+ KR+  ++PG  IL +EP  EA+F+      +K+ A R+  NPEP WGPKARA GNK       G A G
Sbjct:   81 GTEFTPKEYTEEEID---AHLAENAESSGIKIFEALSASGLRSIRYFQQIPGVKSILVNDMDPAAVESIKRNIEFNELPLDRVIPNEDDATDVMYRHRKDKDHFDVIDLDPYGSASIFLDGAVQSVANGGLLCVTCTDMAVLSGKNPEVCFSRYNSIPNKSNYLHEMALRIVLQSIEATANKYARHIVPIAACSIDFYVRVFVRVYKSPVNVKAAMTKLSYTFQCVGCDSFHLQPLGVASGNTFHASRGPVVGQECDQCSGKFKMAGPIWSAPIHDRDVVLRIRERVAKTTTEFP--TKERLHGLLTTISEELVDAPLHYTLPGLSKTLHCSNPRMEQIQGAIRAAGYQASQFHKVPEAIKTNAPNDVIWDIMRCWVKKHPVTKKREGENTPGMRILAKEPKFEANFSSRRPAHEKEKALRFPHNPEPNWGPKARAVGNKAAAPAQNGSAQG 527          
BLAST of mRNA_M-pyrifera_M_contig7789.18317.1 vs. uniprot
Match: A0A5D6XXW9_9STRA (tRNA (guanine(26)-N(2))-dimethyltransferase n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6XXW9_9STRA)

HSP 1 Score: 468 bits (1204), Expect = 3.250e-158
Identity = 239/451 (52.99%), Postives = 322/451 (71.40%), Query Frame = 1
Query:    4 SATQREGAEFTPEDLQQLESTDWGERAVESASTDGIQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAVEAAKRNIEFNQVDSSKMRVQQGDAIAAMHSSIAAKQRFDVIDLDPYGSAAPFLDAAVQAVDDGGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKGRYLHEMALRIVLNALEAQANRYRRHIVPVLSLSIDFYVRLFVRVYTSPAEVKKACTKAAYVHQSLGCGSFYLQPVGKMSKNNYNPSLSTGDGC-CPQTGKRFKIGGPIWAPPMHDVEWVELLLNRVQENPGPFPPTTKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHCDSPRMIDVQAALVNAGYRVSQQHKEPQAVKTDAPDDVVWDIMRCWVKKHPVSDKRK-TSSPGQAILEREPSLEADFTVP-SGLWQKKAATRYQQNPEPYWGPKARATGNKRGVA 1347
            +A  +EGA+FTP++  + +   + E+   +A+T G++I +AL+A+ LRSIRY ++IPGVK ++VND+D AAVE+ KRN+EFN++   ++   + DA   M+       +FDV+DLDPYGSA+ FLD AVQ+V +GGLLCVTCTDM VLSG +PE C+++YG++P K  YLHEMALR VL ++EA AN+Y RHIVP+ S SIDFYVR+FVRV+ SP  VK A TK +YV Q + C SF+LQP+G  + N ++ S     G  C Q G + K+ GPIW+ P+HD + V  +  RV  +   FP  TK+R+HG+LT++SEEL D PL Y+LP +  TLHC +PRM  +Q AL +AGY  SQ HK P+AVKT+AP+DVVWDIMRCWVKKHPV+ KR+  +S GQ IL +EP  EA+F+   +   +K+ A R+ QNPEP WGPKARA GNK   A
Sbjct:   77 AAASQEGADFTPKEYSEDDIDAYLEQ---NAATSGVKIFEALSASGLRSIRYFQQIPGVKSILVNDMDPAAVESIKRNVEFNELPLDRVIPNEDDATNVMYQHRKDADQFDVVDLDPYGSASIFLDGAVQSVSNGGLLCVTCTDMAVLSGKNPEVCFSRYGAIPNKSHYLHEMALRTVLQSIEAAANKYARHIVPIASCSIDFYVRVFVRVFKSPVNVKLAMTKLSYVFQCVSCESFHLQPLGVATGNTFHASRGPVVGPECDQCGGKHKMAGPIWSAPIHDRDVVLRIRERVSASESEFP--TKERLHGLLTTLSEELVDTPLHYTLPGLSKTLHCTNPRMEQMQGALRHAGYEASQFHKVPEAVKTNAPNDVVWDIMRCWVKKHPVTKKREGENSAGQRILAKEPKFEANFSFRRAAAHEKERALRFPQNPEPNWGPKARAVGNKAASA 522          
BLAST of mRNA_M-pyrifera_M_contig7789.18317.1 vs. uniprot
Match: A0A8K1FDP7_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FDP7_PYTOL)

HSP 1 Score: 467 bits (1202), Expect = 6.110e-158
Identity = 240/440 (54.55%), Postives = 316/440 (71.82%), Query Frame = 1
Query:   28 EFTPEDLQQLESTDWGERAVESASTDGIQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAVEAAKRNIEFNQVDSSKMRVQQGDAIAAMHSSIAAKQRFDVIDLDPYGSAAPFLDAAVQAVDDGGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKGRYLHEMALRIVLNALEAQANRYRRHIVPVLSLSIDFYVRLFVRVYTSPAEVKKACTKAAYVHQSLGCGSFYLQPVGKMSKNNYNPSLSTGDGC-CPQTGKRFKIGGPIWAPPMHDVEWVELLLNRVQENPGPFPPTTKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHCDSPRMIDVQAALVNAGYRVSQQHKEPQAVKTDAPDDVVWDIMRCWVKKHPVSDKRK-TSSPGQAILEREPSLEADFTV--PSGLWQKKAATRYQQNPEPYWGPKARATGNK 1335
            EFTP++  + E   +     E+A T GI+I +AL+A+ LRSIRY ++IPGVK ++VND+D AAVE+ KRNIEFN++   ++   + DA   M+S    K +FDVIDLDPYGSA+ FLD AVQ+V +GGLLCVTCTDM VLSG +PE C+++YGS+P K  YLHE ALR+VL+++E+ ANRY+RHIVPV S SIDFYVR+FVRVY SP  VK+A TK +YV Q + C SF+LQ +G  + N ++ S     G  C Q G +FK+ GP W+ P+HD + V  + +RV +    FP  TK R+HG+LT++SEEL D PL Y+LP +C TLHC +PRM  +Q A+ +AGY  SQ HK P A+KT AP+ V+WDIMRCWVKKHP++ KR+   +PG  IL +EP LEA+F+   PS + +K  A R+ QNPEP WGPKARA GNK
Sbjct:   79 EFTPKEYTEEEIDAY---LAETAETSGIRIFEALSASGLRSIRYFQQIPGVKSILVNDMDAAAVESIKRNIEFNELPLDRVIPNEADATDVMYSHRKEKDQFDVIDLDPYGSASIFLDGAVQSVTNGGLLCVTCTDMAVLSGKNPEVCFSRYGSVPNKANYLHENALRMVLHSIESAANRYQRHIVPVASCSIDFYVRVFVRVYKSPVNVKQAMTKLSYVFQCVNCDSFHLQKLGVANGNTFHASRGPVVGQECDQCGGKFKMAGPFWSNPIHDRDVVMRVRDRVAKTTTEFP--TKDRLHGLLTTLSEELVDAPLHYTLPGLCKTLHCSNPRMDQLQGAIRHAGYEASQTHKVPDAIKTTAPNHVIWDIMRCWVKKHPLNKKREGQETPGSRILAKEPQLEANFSSKRPS-VHEKPKALRFPQNPEPNWGPKARAVGNK 512          
BLAST of mRNA_M-pyrifera_M_contig7789.18317.1 vs. uniprot
Match: A0A2D4BF23_PYTIN (tRNA (guanine(26)-N(2))-dimethyltransferase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BF23_PYTIN)

HSP 1 Score: 459 bits (1182), Expect = 3.810e-155
Identity = 238/448 (53.12%), Postives = 314/448 (70.09%), Query Frame = 1
Query:    1 RSATQREGAEFTPEDLQQLESTDWGERAVESASTDGIQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAVEAAKRNIEFNQVDSSKMRVQQGDAIAAMHSSIAAKQRFDVIDLDPYGSAAPFLDAAVQAVDDGGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKGRYLHEMALRIVLNALEAQANRYRRHIVPVLSLSIDFYVRLFVRVYTSPAEVKKACTKAAYVHQSLGCGSFYLQPVGKMSKNNYNPSLSTGDG-CCPQTGKRFKIGGPIWAPPMHDVEWVELLLNRVQENPGPFPPTTKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHCDSPRMIDVQAALVNAGYRVSQQHKEPQAVKTDAPDDVVWDIMRCWVKKHPVSDKRK-TSSPGQAILEREPSLEADFTVPS-GLWQKKAATRYQQNPEPYWGPKARATGNK 1335
            R+A      EFTP +    E     +  +E+A + GI+I  AL+A+ LRSIRY ++IPGVK ++VND+D AAVE+ KRN+EFN++   ++   Q DA   M++     ++FDVIDLDPYGSA+ FLD AVQ+V +GGLLCVTCTDM VLSG +PE C+++YGS+P+K  YLHEMALR+VL ++E+ ANRY+RHIVP+ S SIDFYVR+FVRVY SP  VK+A TK +YV Q + C SF+LQ +G  S N ++ S     G  C Q G +FK+ GP W+ P+HD + V  + +RV      FP  TK+R+HGMLT++SEEL D PL Y+LP +C TLHC +PRM  +Q AL +AGY  SQ HK P A+KT+AP+ V+WDIMRCWVKKHP++ KR+   +PG  IL +EP LEA+F+       +K  A R+  NPEP WGPK RA GNK
Sbjct:   60 RTAAAAAQQEFTPREYSDEEVE---KHLLETAESSGIRI--ALSASGLRSIRYFQQIPGVKSILVNDMDAAAVESIKRNVEFNELPLDRVIPNQADATDVMYNHRKESEQFDVIDLDPYGSASIFLDGAVQSVTNGGLLCVTCTDMAVLSGKNPEVCFSRYGSVPSKANYLHEMALRMVLQSIESAANRYQRHIVPIASCSIDFYVRVFVRVYKSPVNVKQAMTKLSYVFQCVNCDSFHLQKLGVASGNAFHASRGPVVGQSCDQCGGKFKMAGPFWSAPIHDRDVVLRVRDRVASTTTEFP--TKERLHGMLTTISEELVDAPLHYTLPGLCKTLHCSNPRMDQLQGALRHAGYEASQFHKVPDAIKTNAPNHVMWDIMRCWVKKHPLNKKREGQETPGSRILAKEPELEANFSSKRPASHEKPKALRFPMNPEPNWGPKPRAVGNK 500          
BLAST of mRNA_M-pyrifera_M_contig7789.18317.1 vs. uniprot
Match: A0A3M6VG24_9STRA (tRNA (guanine(26)-N(2))-dimethyltransferase n=2 Tax=Peronospora effusa TaxID=542832 RepID=A0A3M6VG24_9STRA)

HSP 1 Score: 458 bits (1179), Expect = 2.300e-154
Identity = 230/423 (54.37%), Postives = 306/423 (72.34%), Query Frame = 1
Query:   85 VESASTDGIQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAVEAAKRNIEFNQVDSSKMRVQQGDAIAAMHSSIAAKQRFDVIDLDPYGSAAPFLDAAVQAVDDGGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKGRYLHEMALRIVLNALEAQANRYRRHIVPVLSLSIDFYVRLFVRVYTSPAEVKKACTKAAYVHQSLGCGSFYLQPVGKMSKNNYNPSLSTGDGC-CPQTGKRFKIGGPIWAPPMHDVEWVELLLNRVQENPGPFPPTTKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHCDSPRMIDVQAALVNAGYRVSQQHKEPQAVKTDAPDDVVWDIMRCWVKKHPVSDKRKTS-SPGQAILEREPSLEADFTVPS-GLWQKKAATRYQQNPEPYWGPKARATGNKRGV 1344
            V++A T+GI+I +ALAA+ LRSIRY ++IPGVK ++VND+D AAVE  KRN+ FN++   ++   + DA   M++      +FDVIDLDPYGSA+ FLD AVQ+V +GGLLCVTCTDM VLSG  PE C+++YG++P K  YLHEMALR+VL+ +E+ ANRY RHIVP+ S SIDFYVR+FVRVY SPA VKKA TK ++V Q + C SF+LQP+G  S N+Y+ S     G  C Q   ++K+ GPIW+ P+H  + V  +L+RV+++   FP  TK R+HG+LT++SEEL D PL+Y+LP +   LHC +PRM  +Q A+ +AGY VSQ HK P A+KT AP+DV+WDIMRCWVKK+P++ KRK   +PG  IL +EP  +A+F+    G  +K  A RY  NPEP WGPKARA G K  +
Sbjct:  101 VDTAETNGIRIFEALAASGLRSIRYFQQIPGVKSILVNDMDPAAVETIKRNVAFNELPLDRVIPNEADATNVMYNHRQHTDQFDVIDLDPYGSASIFLDGAVQSVTNGGLLCVTCTDMPVLSGKQPEVCFSRYGALPNKSHYLHEMALRMVLHTIESSANRYARHIVPIASCSIDFYVRVFVRVYKSPANVKKAMTKLSHVFQCVQCESFHLQPLGVSSGNSYHASRGPVVGQQCDQCQGKYKMAGPIWSAPLHSRDIVLKILDRVEKSTSEFP--TKPRLHGLLTTLSEELIDAPLYYTLPGLSKVLHCTNPRMSQIQGAIRHAGYEVSQFHKVPDAIKTTAPNDVLWDIMRCWVKKYPLNKKRKDQETPGSRILAKEPKFDANFSSKRPGPNEKPKALRYPLNPEPNWGPKARAVGKKADI 521          
BLAST of mRNA_M-pyrifera_M_contig7789.18317.1 vs. uniprot
Match: F0Y1C9_AURAN (Uncharacterized protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y1C9_AURAN)

HSP 1 Score: 452 bits (1164), Expect = 5.100e-154
Identity = 240/416 (57.69%), Postives = 292/416 (70.19%), Query Frame = 1
Query:  109 IQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAVEAAKRNIEFNQVDSSKMRVQQGDAIAAMHSSIA-AKQRFDVIDLDPYGSAAPFLDAAVQAVDDGGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKGRYLHEMALRIVLNALEAQANRYRRHIVPVLSLSIDFYVRLFVRVYTSPAEVKKACTKAAYVHQSLGCGSFYLQPVGKMSKNN-------YNPSLSTGDGCCPQTGKRFKIGGPIWAPPMHDVEWVELLLNRVQENPGPFPPTTKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHCDSPRMIDVQAALVNAGYRVSQQHKEPQAVKTDAPDDVVWDIMRCWVKKHPVSDKR--KTSSPGQAILEREPSLEADFTVPSGLWQKKA-ATRYQQNPEPYWGPKARA 1323
            I++LDALAAT LRSIRY KE+PGV++VV ND+D  A  A  RN   N   +       GDA+  M +S     Q FDVIDLDPYGS APFLDAAVQAV+DGGLLCVT TDM VLSG +PE C+AKYGSMPTK ++LHE ALRI+++A+EA A +Y+RH+VPVLSLSIDFYVR+FVRVY  PAEVKK+  K A+V QS GC SFYLQP+ +             +   +     C +TG  FKIGGPIW+ P+HD EWV   L+RV         TT+ RIHGMLT+VSEEL DVPL+Y+LPD+CS LHC SP+M +V+AAL +AGYRVSQ H+EP A+KTDAP  V+WD+ RCWVKKHPVS+KR  +  S    IL   P LEA+F   + +  K   A R+  NPE +WGPKA A
Sbjct:    1 IRVLDALAATGLRSIRYAKEVPGVRKVVANDVDADAAAACGRNAAENGA-ADICEAHCGDAVDFMTASRGKGSQGFDVIDLDPYGSCAPFLDAAVQAVNDGGLLCVTSTDMTVLSGNYPEVCFAKYGSMPTKAKHLHEFALRILVHAIEAAAAKYQRHVVPVLSLSIDFYVRVFVRVYKRPAEVKKSAAKRAFVLQSAGCPSFYLQPLARSLDREGGHAAALLHRKATVAPAFCGETGAPFKIGGPIWSAPLHDPEWVLAGLDRVAAGVDAHLSTTE-RIHGMLTAVSEELLDVPLYYTLPDLCSALHCASPKMAEVRAALTHAGYRVSQHHREPDAIKTDAPPRVLWDVFRCWVKKHPVSEKRLAEPRSAAAKILATAPVLEANFATTAAMRAKATKARRWAPNPEEHWGPKAAA 414          
BLAST of mRNA_M-pyrifera_M_contig7789.18317.1 vs. uniprot
Match: A0A067CL21_SAPPC (tRNA (guanine(26)-N(2))-dimethyltransferase n=2 Tax=Saprolegnia TaxID=4769 RepID=A0A067CL21_SAPPC)

HSP 1 Score: 454 bits (1169), Expect = 3.630e-153
Identity = 227/415 (54.70%), Postives = 300/415 (72.29%), Query Frame = 1
Query:   94 ASTDGIQILDALAATALRSIRYVKEIPGVKEVVVNDLDEAAVEAAKRNIEFNQVDSSKMRVQQGDAIAAMHSSIAAKQRFDVIDLDPYGSAAPFLDAAVQAVDDGGLLCVTCTDMGVLSGGHPETCYAKYGSMPTKGRYLHEMALRIVLNALEAQANRYRRHIVPVLSLSIDFYVRLFVRVYTSPAEVKKACTKAAYVHQSLGCGSFYLQPVGKMSKNNYNPSLSTGD-GCCPQTGKRFKIGGPIWAPPMHDVEWV-ELLLNRVQENPGPFPPTTKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHCDSPRMIDVQAALVNAGYRVSQQHKEPQAVKTDAPDDVVWDIMRCWVKKHPVSDKRKTSSP-GQAILEREPSLEADFTVPSGLWQKKAATRYQQNPEPYWGPKARATG 1329
            A T G++I +AL+A+ LRSIRY +EI GVK ++VNDLD AAV + KRNIEFN + + K+   + DA + M+        FDVIDLDPYGSA+ FLD AVQA+ +GGLLCVTCTDM VLSG  P+ C+ +YGS+P K  YLHE ALR+VL+++E+ A RY++HIVP++S SIDFYVR+FVRVY SP  VK + TK +YV+Q  GC SF++QP+GK +   Y+ S    +   C Q GK+FK+ GPIW+ P+HD + V ++  N ++E    +P  TK R+HG+LTSVSEE+ D PLFY+L  +C TLHC  PRM  +Q+A++NAGY+VSQ HK P+A+KTDAP+DV+WDIMRCWVKKHP+S K ++    G  IL +EP  EA FT       K+ A R+ +NPE YWGPK+RA G
Sbjct:   88 AETSGLKIFEALSASGLRSIRYWQEIAGVKSILVNDLDPAAVISIKRNIEFNGLTTDKLIPNEDDATSVMYRHRKEADNFDVIDLDPYGSASIFLDGAVQAIANGGLLCVTCTDMPVLSGKDPDVCFHRYGSVPYKSNYLHENALRMVLHSIESAAIRYQKHIVPIVSCSIDFYVRVFVRVYKSPVNVKASMTKLSYVYQCTGCDSFHMQPLGKFNGKTYHASFVEPERALCDQCGKKFKMSGPIWSNPLHDRDLVLKIRENVIKEGVAKYP--TKDRLHGLLTSVSEEVVDAPLFYTLHGICKTLHCGQPRMDQMQSAILNAGYKVSQSHKVPEAIKTDAPNDVIWDIMRCWVKKHPISKKHESEKDAGTQILAKEPKHEAVFTGKRPSAGKEKALRFPKNPEAYWGPKSRARG 500          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig7789.18317.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LH70_ECTSI1.710e-29383.80tRNA (guanine(26)-N(2))-dimethyltransferase n=2 Ta... [more]
W7T9F0_9STRA4.900e-18359.17tRNA (guanine(26)-N(2))-dimethyltransferase n=2 Ta... [more]
A0A836C8Z8_9STRA1.510e-17759.48tRNA (guanine(26)-N(2))-dimethyltransferase n=1 Ta... [more]
K3WLD8_GLOUD4.840e-15953.10tRNA (guanine(26)-N(2))-dimethyltransferase n=1 Ta... [more]
A0A5D6XXW9_9STRA3.250e-15852.99tRNA (guanine(26)-N(2))-dimethyltransferase n=1 Ta... [more]
A0A8K1FDP7_PYTOL6.110e-15854.55Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A2D4BF23_PYTIN3.810e-15553.13tRNA (guanine(26)-N(2))-dimethyltransferase n=1 Ta... [more]
A0A3M6VG24_9STRA2.300e-15454.37tRNA (guanine(26)-N(2))-dimethyltransferase n=2 Ta... [more]
F0Y1C9_AURAN5.100e-15457.69Uncharacterized protein (Fragment) n=1 Tax=Aureoco... [more]
A0A067CL21_SAPPC3.630e-15354.70tRNA (guanine(26)-N(2))-dimethyltransferase n=2 Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig7789contigM-pyrifera_M_contig7789:9456..10862 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score820.1
Seed ortholog evalue5.4e-235
Seed eggNOG ortholog2880.D8LH70
Preferred nameTRMT1
KEGG rclassRC00034,RC00099,RC00108,RC00133,RC00205,RC00670,RC00767,RC02141,RC02755
KEGG koko:K00011,ko:K00555,ko:K03781
KEGG ReactionR00009,R00602,R01036,R01041,R01093,R01095,R01431,R01758,R01759,R01787,R02531,R02577,R02670,R04285,R11764
KEGG Pathwayko00040,ko00051,ko00052,ko00380,ko00561,ko00630,ko00790,ko01100,ko01110,ko01130,ko01200,ko04011,ko04016,ko04068,ko04146,ko04211,ko04212,ko04213,ko05014,map00040,map00051,map00052,map00380,map00561,map00630,map00790,map01100,map01110,map01130,map01200,map04011,map04016,map04068,map04146,map04211,map04212,map04213,map05014
KEGG ModuleM00532
Hectar predicted targeting categoryother localisation
GOsGO:0001510,GO:0002940,GO:0003674,GO:0003824,GO:0004809,GO:0005575,GO:0005622,GO:0005623,GO:0005634,GO:0005635,GO:0005637,GO:0005654,GO:0005737,GO:0005739,GO:0006139,GO:0006396,GO:0006399,GO:0006400,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0008168,GO:0008170,GO:0008173,GO:0008175,GO:0008757,GO:0009451,GO:0009987,GO:0010467,GO:0012505,GO:0016020,GO:0016070,GO:0016423,GO:0016740,GO:0016741,GO:0019866,GO:0030488,GO:0031090,GO:0031965,GO:0031967,GO:0031974,GO:0031975,GO:0031981,GO:0032259,GO:0034470,GO:0034641,GO:0034660,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0043414,GO:0044237,GO:0044238,GO:0044260,GO:0044422,GO:0044424,GO:0044428,GO:0044444,GO:0044446,GO:0044464,GO:0046483,GO:0070013,GO:0071704,GO:0090304,GO:0140098,GO:0140101,GO:1901360
EggNOG free text desc.N(2),N(2)-dimethylguanosine tRNA methyltransferase
EggNOG OGsCOG1867@1,KOG1253@2759
Ec32 ortholog descriptiontRNA methyltransferase, Trm1
Ec32 orthologEc-24_000780.1
EC1.1.1.21,1.11.1.6,2.1.1.215,2.1.1.216
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03016
Exons1
Model size1407
Cds size1407
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816679.9787235-CDS-M-pyrifera_M_contig7789:9455..108621622816679.9787235-CDS-M-pyrifera_M_contig7789:9455..10862Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig7789 9456..10862 +
1692278115.5905192-CDS-M-pyrifera_M_contig7789:9455..108621692278115.5905192-CDS-M-pyrifera_M_contig7789:9455..10862Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig7789 9456..10862 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig7789.18317.1prot_M-pyrifera_M_contig7789.18317.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig7789 9456..10862 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig7789.18317.1

>prot_M-pyrifera_M_contig7789.18317.1 ID=prot_M-pyrifera_M_contig7789.18317.1|Name=mRNA_M-pyrifera_M_contig7789.18317.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=469bp
RSATQREGAEFTPEDLQQLESTDWGERAVESASTDGIQILDALAATALRS
IRYVKEIPGVKEVVVNDLDEAAVEAAKRNIEFNQVDSSKMRVQQGDAIAA
MHSSIAAKQRFDVIDLDPYGSAAPFLDAAVQAVDDGGLLCVTCTDMGVLS
GGHPETCYAKYGSMPTKGRYLHEMALRIVLNALEAQANRYRRHIVPVLSL
SIDFYVRLFVRVYTSPAEVKKACTKAAYVHQSLGCGSFYLQPVGKMSKNN
YNPSLSTGDGCCPQTGKRFKIGGPIWAPPMHDVEWVELLLNRVQENPGPF
PPTTKKRIHGMLTSVSEELPDVPLFYSLPDMCSTLHCDSPRMIDVQAALV
NAGYRVSQQHKEPQAVKTDAPDDVVWDIMRCWVKKHPVSDKRKTSSPGQA
ILEREPSLEADFTVPSGLWQKKAATRYQQNPEPYWGPKARATGNKRGVAD
GGGPADGTPAGKKKKPAES
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mRNA from alignment at M-pyrifera_M_contig7789:9456..10862+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig7789.18317.1 ID=mRNA_M-pyrifera_M_contig7789.18317.1|Name=mRNA_M-pyrifera_M_contig7789.18317.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=1407bp|location=Sequence derived from alignment at M-pyrifera_M_contig7789:9456..10862+ (Macrocystis pyrifera P11B4 male)
CGGTCCGCAACGCAGAGAGAAGGGGCGGAGTTCACGCCAGAAGACTTGCA ACAGTTGGAGTCAACGGACTGGGGCGAGCGTGCTGTAGAAAGTGCCTCGA CGGATGGGATACAAATCCTCGATGCGCTAGCCGCGACTGCCTTGCGCTCC ATCAGGTACGTCAAGGAGATCCCCGGGGTTAAGGAGGTGGTTGTCAATGA CCTGGACGAGGCGGCGGTAGAGGCTGCAAAGAGAAACATCGAATTCAACC AGGTGGATTCCTCCAAGATGCGGGTGCAGCAGGGGGATGCCATTGCCGCC ATGCACTCGAGCATTGCGGCCAAACAGCGCTTCGACGTCATCGATCTAGA CCCTTACGGATCTGCTGCTCCATTTTTGGATGCAGCCGTGCAGGCGGTGG ATGACGGAGGGCTTTTATGCGTAACCTGCACCGATATGGGCGTTCTCAGC GGGGGGCACCCGGAAACTTGTTATGCCAAGTATGGCAGCATGCCGACGAA AGGAAGGTATTTGCACGAGATGGCTCTTCGGATAGTCCTCAATGCCCTGG AAGCGCAAGCCAATCGCTACCGTCGCCATATTGTGCCGGTACTCAGTCTT AGCATCGACTTCTACGTGCGACTGTTCGTTCGGGTGTACACGTCTCCGGC CGAGGTGAAGAAGGCCTGTACGAAGGCGGCATACGTACACCAGAGCTTGG GTTGCGGTTCGTTCTACCTTCAACCGGTTGGCAAGATGTCGAAAAACAAC TACAACCCTTCGCTCTCCACAGGAGACGGTTGCTGCCCTCAAACTGGGAA GCGGTTCAAGATCGGCGGGCCTATCTGGGCCCCGCCCATGCACGACGTGG AGTGGGTGGAACTGCTGTTGAACCGGGTCCAGGAGAATCCGGGCCCGTTT CCGCCTACCACGAAGAAGCGAATCCACGGCATGCTTACTTCCGTGTCGGA AGAACTGCCGGATGTCCCTCTCTTCTACAGCTTGCCTGACATGTGCTCGA CCCTTCACTGCGATAGTCCGCGGATGATTGACGTGCAGGCTGCGCTGGTG AACGCGGGATACAGGGTATCTCAACAGCACAAGGAACCGCAGGCCGTGAA GACGGATGCCCCGGACGACGTCGTGTGGGACATCATGCGTTGCTGGGTGA AGAAGCACCCAGTTAGCGACAAGCGCAAGACCAGCTCACCCGGACAGGCA ATTTTGGAGCGTGAACCCTCTCTTGAAGCGGACTTCACAGTCCCTTCTGG TCTGTGGCAGAAAAAAGCAGCCACCAGGTATCAGCAGAACCCGGAACCCT ACTGGGGGCCAAAGGCTCGCGCGACTGGAAACAAACGAGGGGTTGCGGAT GGTGGCGGGCCTGCAGATGGCACACCCGCTGGGAAGAAGAAGAAACCTGC AGAATCA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig7789:9456..10862+

>mRNA_M-pyrifera_M_contig7789.18317.1 ID=mRNA_M-pyrifera_M_contig7789.18317.1|Name=mRNA_M-pyrifera_M_contig7789.18317.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=2814bp|location=Sequence derived from alignment at M-pyrifera_M_contig7789:9456..10862+ (Macrocystis pyrifera P11B4 male)
CGGTCCGCAACGCAGAGAGAAGGGGCGGAGTTCACGCCAGAAGACTTGCA
ACAGTTGGAGTCAACGGACTGGGGCGAGCGTGCTGTAGAAAGTGCCTCGA
CGGATGGGATACAAATCCTCGATGCGCTAGCCGCGACTGCCTTGCGCTCC
ATCAGGTACGTCAAGGAGATCCCCGGGGTTAAGGAGGTGGTTGTCAATGA
CCTGGACGAGGCGGCGGTAGAGGCTGCAAAGAGAAACATCGAATTCAACC
AGGTGGATTCCTCCAAGATGCGGGTGCAGCAGGGGGATGCCATTGCCGCC
ATGCACTCGAGCATTGCGGCCAAACAGCGCTTCGACGTCATCGATCTAGA
CCCTTACGGATCTGCTGCTCCATTTTTGGATGCAGCCGTGCAGGCGGTGG
ATGACGGAGGGCTTTTATGCGTAACCTGCACCGATATGGGCGTTCTCAGC
GGGGGGCACCCGGAAACTTGTTATGCCAAGTATGGCAGCATGCCGACGAA
AGGAAGGTATTTGCACGAGATGGCTCTTCGGATAGTCCTCAATGCCCTGG
AAGCGCAAGCCAATCGCTACCGTCGCCATATTGTGCCGGTACTCAGTCTT
AGCATCGACTTCTACGTGCGACTGTTCGTTCGGGTGTACACGTCTCCGGC
CGAGGTGAAGAAGGCCTGTACGAAGGCGGCATACGTACACCAGAGCTTGG
GTTGCGGTTCGTTCTACCTTCAACCGGTTGGCAAGATGTCGAAAAACAAC
TACAACCCTTCGCTCTCCACAGGAGACGGTTGCTGCCCTCAAACTGGGAA
GCGGTTCAAGATCGGCGGGCCTATCTGGGCCCCGCCCATGCACGACGTGG
AGTGGGTGGAACTGCTGTTGAACCGGGTCCAGGAGAATCCGGGCCCGTTT
CCGCCTACCACGAAGAAGCGAATCCACGGCATGCTTACTTCCGTGTCGGA
AGAACTGCCGGATGTCCCTCTCTTCTACAGCTTGCCTGACATGTGCTCGA
CCCTTCACTGCGATAGTCCGCGGATGATTGACGTGCAGGCTGCGCTGGTG
AACGCGGGATACAGGGTATCTCAACAGCACAAGGAACCGCAGGCCGTGAA
GACGGATGCCCCGGACGACGTCGTGTGGGACATCATGCGTTGCTGGGTGA
AGAAGCACCCAGTTAGCGACAAGCGCAAGACCAGCTCACCCGGACAGGCA
ATTTTGGAGCGTGAACCCTCTCTTGAAGCGGACTTCACAGTCCCTTCTGG
TCTGTGGCAGAAAAAAGCAGCCACCAGGTATCAGCAGAACCCGGAACCCT
ACTGGGGGCCAAAGGCTCGCGCGACTGGAAACAAACGAGGGGTTGCGGAT
GGTGGCGGGCCTGCAGATGGCACACCCGCTGGGAAGAAGAAGAAACCTGC
AGAATCACGGTCCGCAACGCAGAGAGAAGGGGCGGAGTTCACGCCAGAAG
ACTTGCAACAGTTGGAGTCAACGGACTGGGGCGAGCGTGCTGTAGAAAGT
GCCTCGACGGATGGGATACAAATCCTCGATGCGCTAGCCGCGACTGCCTT
GCGCTCCATCAGGTACGTCAAGGAGATCCCCGGGGTTAAGGAGGTGGTTG
TCAATGACCTGGACGAGGCGGCGGTAGAGGCTGCAAAGAGAAACATCGAA
TTCAACCAGGTGGATTCCTCCAAGATGCGGGTGCAGCAGGGGGATGCCAT
TGCCGCCATGCACTCGAGCATTGCGGCCAAACAGCGCTTCGACGTCATCG
ATCTAGACCCTTACGGATCTGCTGCTCCATTTTTGGATGCAGCCGTGCAG
GCGGTGGATGACGGAGGGCTTTTATGCGTAACCTGCACCGATATGGGCGT
TCTCAGCGGGGGGCACCCGGAAACTTGTTATGCCAAGTATGGCAGCATGC
CGACGAAAGGAAGGTATTTGCACGAGATGGCTCTTCGGATAGTCCTCAAT
GCCCTGGAAGCGCAAGCCAATCGCTACCGTCGCCATATTGTGCCGGTACT
CAGTCTTAGCATCGACTTCTACGTGCGACTGTTCGTTCGGGTGTACACGT
CTCCGGCCGAGGTGAAGAAGGCCTGTACGAAGGCGGCATACGTACACCAG
AGCTTGGGTTGCGGTTCGTTCTACCTTCAACCGGTTGGCAAGATGTCGAA
AAACAACTACAACCCTTCGCTCTCCACAGGAGACGGTTGCTGCCCTCAAA
CTGGGAAGCGGTTCAAGATCGGCGGGCCTATCTGGGCCCCGCCCATGCAC
GACGTGGAGTGGGTGGAACTGCTGTTGAACCGGGTCCAGGAGAATCCGGG
CCCGTTTCCGCCTACCACGAAGAAGCGAATCCACGGCATGCTTACTTCCG
TGTCGGAAGAACTGCCGGATGTCCCTCTCTTCTACAGCTTGCCTGACATG
TGCTCGACCCTTCACTGCGATAGTCCGCGGATGATTGACGTGCAGGCTGC
GCTGGTGAACGCGGGATACAGGGTATCTCAACAGCACAAGGAACCGCAGG
CCGTGAAGACGGATGCCCCGGACGACGTCGTGTGGGACATCATGCGTTGC
TGGGTGAAGAAGCACCCAGTTAGCGACAAGCGCAAGACCAGCTCACCCGG
ACAGGCAATTTTGGAGCGTGAACCCTCTCTTGAAGCGGACTTCACAGTCC
CTTCTGGTCTGTGGCAGAAAAAAGCAGCCACCAGGTATCAGCAGAACCCG
GAACCCTACTGGGGGCCAAAGGCTCGCGCGACTGGAAACAAACGAGGGGT
TGCGGATGGTGGCGGGCCTGCAGATGGCACACCCGCTGGGAAGAAGAAGA
AACCTGCAGAATCA
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