mRNA_M-pyrifera_M_contig77202.18197.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig77202.18197.1
Unique NamemRNA_M-pyrifera_M_contig77202.18197.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig77202.18197.1 vs. uniprot
Match: A0A6H5KR44_9PHAE (Lipoyl-binding domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KR44_9PHAE)

HSP 1 Score: 86.3 bits (212), Expect = 4.390e-18
Identity = 47/64 (73.44%), Postives = 50/64 (78.12%), Query Frame = 1 
Query:    1 TVDFDAQDDSFMAKVLVDAGTQDIPVGTPIFVTVDDTEFVAAFKDFVV-AAESEPETVEPVAAA 189
            TVDFDAQDDSFMAK LV+AGTQDI VGTPIFVTVDD + VAAFKDF   A + EPE   P A A
Sbjct:   89 TVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDFEAEAVQGEPEAAAPAAEA 152
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BLAST of mRNA_M-pyrifera_M_contig77202.18197.1 vs. uniprot
Match: D8LTQ3_ECTSI (Lipoyl-binding domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LTQ3_ECTSI)

HSP 1 Score: 82.0 bits (201), Expect = 1.140e-16
Identity = 47/63 (74.60%), Postives = 50/63 (79.37%), Query Frame = 1 
Query:    1 TVDFDAQDDSFMAKVLVDAGTQDIPVGTPIFVTVDDTEFVAAFKDFVVAAESEPETVEPVAAA 189
            TVDFDAQDDSFMAK LV+AGTQDI VGTPIFVTVDD + VAAFKDF    E+E    EP AAA
Sbjct:   61 TVDFDAQDDSFMAKHLVEAGTQDIAVGTPIFVTVDDADSVAAFKDF----EAEAVQGEPEAAA 119
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BLAST of mRNA_M-pyrifera_M_contig77202.18197.1 vs. uniprot
Match: A0A7R9WUM2_9STRA (Hypothetical protein n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A7R9WUM2_9STRA)

HSP 1 Score: 72.0 bits (175), Expect = 1.700e-12
Identity = 37/63 (58.73%), Postives = 47/63 (74.60%), Query Frame = 1 
Query:    1 TVDFDAQDDSFMAKVLVDAGTQDIPVGTPIFVTVDDTEFVAAFKDFVVAAESEPETVEPVAAA 189
            ++DF+AQDD ++AK+L DAG +D+PVGTPI VTV++ E VAAFKDFV  A       EP AAA
Sbjct:  143 SIDFEAQDDGYIAKILKDAGAEDVPVGTPILVTVEEEEDVAAFKDFVAPA------AEPEAAA 199
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BLAST of mRNA_M-pyrifera_M_contig77202.18197.1 vs. uniprot
Match: D8LL61_ECTSI (Dihydrolipoamide acetyltransferase (Partial) (Fragment) n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LL61_ECTSI)

HSP 1 Score: 67.0 bits (162), Expect = 5.550e-11
Identity = 36/62 (58.06%), Postives = 45/62 (72.58%), Query Frame = 1 
Query:    1 TVDFDAQDDSFMAKVLVDAGTQDIPVGTPIFVTVDDTEFVAAFKDFVVAAESEPETVEPVAA 186
            TVDF+AQD++ +AK+LV AGT D+ VGTP+ V  + T+ VAAFKDF   A  E ET  PVAA
Sbjct:  106 TVDFEAQDEAVLAKILVPAGTPDVAVGTPMMVLTESTDDVAAFKDFSAGA-PETETAAPVAA 166
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BLAST of mRNA_M-pyrifera_M_contig77202.18197.1 vs. uniprot
Match: A0A7S2DCZ1_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Haptolina brevifila TaxID=156173 RepID=A0A7S2DCZ1_9EUKA)

HSP 1 Score: 65.1 bits (157), Expect = 1.230e-10
Identity = 33/62 (53.23%), Postives = 42/62 (67.74%), Query Frame = 1 
Query:    1 TVDFDAQDDSFMAKVLVDAGTQDIPVGTPIFVTVDDTEFVAAFKDFVVAAESEPETVEPVAA 186
            TVD++AQD+ F+AK+LV  G  D+ VGTP+ V VDD   +AAFKDF   A + P   EP AA
Sbjct:   76 TVDYEAQDEGFIAKILVQEGASDVAVGTPVAVVVDDEADIAAFKDFTATAAASP---EPAAA 134
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BLAST of mRNA_M-pyrifera_M_contig77202.18197.1 vs. uniprot
Match: A0A2R5GK75_9STRA (Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GK75_9STRA)

HSP 1 Score: 64.3 bits (155), Expect = 7.000e-10
Identity = 31/49 (63.27%), Postives = 38/49 (77.55%), Query Frame = 1 
Query:    1 TVDFDAQDDSFMAKVLVDAGTQDIPVGTPIFVTVDDTEFVAAFKDFVVA 147
            TVDF+AQDD F+AK+LV+AGT D+ VGTPI V V+D   + AFKDF  A
Sbjct:   84 TVDFEAQDDGFLAKILVEAGTSDVAVGTPIGVMVEDEGDIEAFKDFTAA 132
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BLAST of mRNA_M-pyrifera_M_contig77202.18197.1 vs. uniprot
Match: A0A7S2SNE1_9STRA (Hypothetical protein n=2 Tax=labyrinthulid quahog parasite QPX TaxID=96639 RepID=A0A7S2SNE1_9STRA)

HSP 1 Score: 62.8 bits (151), Expect = 2.230e-9
Identity = 29/49 (59.18%), Postives = 38/49 (77.55%), Query Frame = 1 
Query:    1 TVDFDAQDDSFMAKVLVDAGTQDIPVGTPIFVTVDDTEFVAAFKDFVVA 147
            TVDF+AQDD F+AK+L+DAG  D+PVGTPI V V++   +AAF +F  A
Sbjct:   73 TVDFEAQDDGFLAKILMDAGASDVPVGTPIGVVVEEEADIAAFANFTAA 121
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BLAST of mRNA_M-pyrifera_M_contig77202.18197.1 vs. uniprot
Match: A0A6H5KLJ3_9PHAE (Acetyltransferase component of pyruvate dehydrogenase complex n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KLJ3_9PHAE)

HSP 1 Score: 63.9 bits (154), Expect = 2.340e-9
Identity = 35/63 (55.56%), Postives = 44/63 (69.84%), Query Frame = 1 
Query:    1 TVDFDAQDDSFMAKVLVDAGTQDIPVGTPIFVTVDDTEFVAAFKDFVVAAESEPETVEPVAAA 189
            TVDF+AQD++ +AK+LV AGT D+ VGTP+ V  + T+ VAAF DF   A  E ET  P AAA
Sbjct:  133 TVDFEAQDEAVLAKILVPAGTPDVAVGTPMMVLTESTDDVAAFADFSAGA-PETETAAPPAAA 194
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BLAST of mRNA_M-pyrifera_M_contig77202.18197.1 vs. uniprot
Match: A0A8J2X464_ZYGB2 (Acetyltransferase component of pyruvate dehydrogenase complex n=2 Tax=Zygosaccharomyces TaxID=4953 RepID=A0A8J2X464_ZYGB2)

HSP 1 Score: 62.8 bits (151), Expect = 5.780e-9
Identity = 30/48 (62.50%), Postives = 39/48 (81.25%), Query Frame = 1 
Query:    1 TVDFDAQDDSFMAKVLVDAGTQDIPVGTPIFVTVDDTEFVAAFKDFVV 144
            T+DF+ QDD+++AK+L+ AGT+DIPVG PI VTV+D   V AFKDF V
Sbjct:   74 TMDFEYQDDAYLAKILIPAGTKDIPVGKPIAVTVEDAADVDAFKDFKV 121
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BLAST of mRNA_M-pyrifera_M_contig77202.18197.1 vs. uniprot
Match: A0A7S1YNN2_9STRA (Hypothetical protein (Fragment) n=1 Tax=Skeletonema marinoi TaxID=267567 RepID=A0A7S1YNN2_9STRA)

HSP 1 Score: 60.5 bits (145), Expect = 8.440e-9
Identity = 30/50 (60.00%), Postives = 38/50 (76.00%), Query Frame = 1 
Query:    1 TVDFDAQDDSFMAKVLVDAGTQDIPVGTPIFVTVDDTEFVAAFKDFVVAA 150
            TVDF+AQD+  +AK++V AG  +IP GTPI VTV+D   V +FKDFVV A
Sbjct:   93 TVDFEAQDEGVVAKIIVQAGGGEIPCGTPILVTVEDEADVPSFKDFVVEA 142
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig77202.18197.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6H5KR44_9PHAE4.390e-1873.44Lipoyl-binding domain-containing protein n=1 Tax=E... [more]
D8LTQ3_ECTSI1.140e-1674.60Lipoyl-binding domain-containing protein n=1 Tax=E... [more]
A0A7R9WUM2_9STRA1.700e-1258.73Hypothetical protein n=1 Tax=Craspedostauros austr... [more]
D8LL61_ECTSI5.550e-1158.06Dihydrolipoamide acetyltransferase (Partial) (Frag... [more]
A0A7S2DCZ1_9EUKA1.230e-1053.23Hypothetical protein (Fragment) n=1 Tax=Haptolina ... [more]
A0A2R5GK75_9STRA7.000e-1063.27Dihydrolipoyllysine-residue acetyltransferase comp... [more]
A0A7S2SNE1_9STRA2.230e-959.18Hypothetical protein n=2 Tax=labyrinthulid quahog ... [more]
A0A6H5KLJ3_9PHAE2.340e-955.56Acetyltransferase component of pyruvate dehydrogen... [more]
A0A8J2X464_ZYGB25.780e-962.50Acetyltransferase component of pyruvate dehydrogen... [more]
A0A7S1YNN2_9STRA8.440e-960.00Hypothetical protein (Fragment) n=1 Tax=Skeletonem... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig77202contigM-pyrifera_M_contig77202:575..952 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score82.8
Seed ortholog evalue1.3e-13
Seed eggNOG ortholog2880.D8LTQ3
KEGG rclassRC00004,RC02742,RC02857
KEGG koko:K00627,ko:K13997
KEGG ReactionR00209,R02569
KEGG Pathwayko00010,ko00020,ko00620,ko01100,ko01110,ko01120,ko01130,ko01200,map00010,map00020,map00620,map01100,map01110,map01120,map01130,map01200
KEGG ModuleM00307
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0006082,GO:0006084,GO:0006085,GO:0006086,GO:0006090,GO:0006139,GO:0006163,GO:0006164,GO:0006637,GO:0006725,GO:0006732,GO:0006753,GO:0006790,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009108,GO:0009117,GO:0009150,GO:0009152,GO:0009165,GO:0009259,GO:0009260,GO:0009987,GO:0018130,GO:0019438,GO:0019637,GO:0019693,GO:0019752,GO:0032787,GO:0032991,GO:0033865,GO:0033866,GO:0033875,GO:0034030,GO:0034032,GO:0034033,GO:0034641,GO:0034654,GO:0035383,GO:0035384,GO:0043436,GO:0043603,GO:0043604,GO:0044237,GO:0044238,GO:0044249,GO:0044271,GO:0044272,GO:0044281,GO:0044424,GO:0044444,GO:0044464,GO:0045254,GO:0046390,GO:0046483,GO:0051186,GO:0051188,GO:0055086,GO:0071616,GO:0071704,GO:0072521,GO:0072522,GO:0090407,GO:1901135,GO:1901137,GO:1901293,GO:1901360,GO:1901362,GO:1901564,GO:1901566,GO:1901576,GO:1902494,GO:1990204
EggNOG free text desc.dihydrolipoyllysine-residue acetyltransferase activity
EggNOG OGsCOG0508@1,KOG0557@2759
EC2.3.1.12
COG Functional cat.C
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEbr01601,ko00000,ko00001,ko00002,ko01000
Exons1
Model size378
Cds size378
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816673.445809-CDS-M-pyrifera_M_contig77202:574..9521622816673.445809-CDS-M-pyrifera_M_contig77202:574..952Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig77202 575..952 -
1692278111.6369503-CDS-M-pyrifera_M_contig77202:574..9521692278111.6369503-CDS-M-pyrifera_M_contig77202:574..952Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig77202 575..952 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig77202.18197.1prot_M-pyrifera_M_contig77202.18197.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig77202 575..952 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig77202.18197.1

>prot_M-pyrifera_M_contig77202.18197.1 ID=prot_M-pyrifera_M_contig77202.18197.1|Name=mRNA_M-pyrifera_M_contig77202.18197.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=126bp
TVDFDAQDDSFMAKVLVDAGTQDIPVGTPIFVTVDDTEFVAAFKDFVVAA
ESEPETVEPVAAAEAAPAPIVVAEKEVVVAAAAAAAAAPAPVPAAAAVAA
AAEPVPVAVEAPGEFPECCFFGGRG*
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mRNA from alignment at M-pyrifera_M_contig77202:575..952-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig77202.18197.1 ID=mRNA_M-pyrifera_M_contig77202.18197.1|Name=mRNA_M-pyrifera_M_contig77202.18197.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=378bp|location=Sequence derived from alignment at M-pyrifera_M_contig77202:575..952- (Macrocystis pyrifera P11B4 male)
ACCGTAGATTTCGACGCGCAGGACGACTCCTTCATGGCGAAGGTCCTGGT
GGATGCGGGCACCCAGGACATCCCGGTGGGCACTCCAATCTTCGTCACCG
TAGATGACACCGAATTTGTCGCGGCCTTCAAGGACTTCGTGGTTGCGGCG
GAATCTGAACCTGAAACCGTCGAACCCGTCGCTGCTGCCGAAGCGGCACC
GGCTCCAATTGTCGTGGCGGAGAAGGAGGTAGTAGTGGCGGCAGCGGCGG
CGGCAGCGGCGGCTCCTGCGCCGGTACCGGCGGCGGCGGCGGTGGCGGCG
GCGGCGGAACCGGTGCCGGTTGCGGTGGAGGCGCCTGGTGAGTTCCCGGA
GTGTTGTTTTTTTGGGGGGAGGGGGTAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig77202:575..952-

>mRNA_M-pyrifera_M_contig77202.18197.1 ID=mRNA_M-pyrifera_M_contig77202.18197.1|Name=mRNA_M-pyrifera_M_contig77202.18197.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=756bp|location=Sequence derived from alignment at M-pyrifera_M_contig77202:575..952- (Macrocystis pyrifera P11B4 male)
ACCGTAGATTTCGACGCGCAGGACGACTCCTTCATGGCGAAGGTCCTGGT
GGATGCGGGCACCCAGGACATCCCGGTGGGCACTCCAATCTTCGTCACCG
TAGATGACACCGAATTTGTCGCGGCCTTCAAGGACTTCGTGGTTGCGGCG
GAATCTGAACCTGAAACCGTCGAACCCGTCGCTGCTGCCGAAGCGGCACC
GGCTCCAATTGTCGTGGCGGAGAAGGAGGTAGTAGTGGCGGCAGCGGCGG
CGGCAGCGGCGGCTCCTGCGCCGGTACCGGCGGCGGCGGCGGTGGCGGCG
GCGGCGGAACCGGTGCCGGTTGCGGTGGAGGCGCCTGGTGAGTTCCCGGA
GTGTTGTTTTTTTGGGGGGAGGGGGTAGACCGTAGATTTCGACGCGCAGG
ACGACTCCTTCATGGCGAAGGTCCTGGTGGATGCGGGCACCCAGGACATC
CCGGTGGGCACTCCAATCTTCGTCACCGTAGATGACACCGAATTTGTCGC
GGCCTTCAAGGACTTCGTGGTTGCGGCGGAATCTGAACCTGAAACCGTCG
AACCCGTCGCTGCTGCCGAAGCGGCACCGGCTCCAATTGTCGTGGCGGAG
AAGGAGGTAGTAGTGGCGGCAGCGGCGGCGGCAGCGGCGGCTCCTGCGCC
GGTACCGGCGGCGGCGGCGGTGGCGGCGGCGGCGGAACCGGTGCCGGTTG
CGGTGGAGGCGCCTGGTGAGTTCCCGGAGTGTTGTTTTTTTGGGGGGAGG
GGGTAG
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